Transient Method Research Articles

Study on transient prediction method of dynamic underbalanced pressure after perforation detonation in ultra-deep wells

Dynamic underbalanced pressure creates a significant transient pressure difference between the upper and lower ends of the packer and the inner and outer walls of the string below the packer, which can lead to the failure of the packer, the tubing, and the casing and is one of the main reasons for the low success rate of the perforation-acidizing-testing combined technology in ultra-deep wells. A danger caused by the dynamic underbalanced pressure is increased rapidly with an increase in the well depth. Despite the fact that researchers have a well enough understanding of the applications and benefits of the dynamic underbalanced pressure, the mechanism of the wellbore failure caused by the dynamic underbalanced pressure is inadequate. Therefore, a pressure drop model of tubing-casing annulus fluid is established, and a seepage model of reservoir rock is given. A transient prediction method of the dynamic underbalanced pressure is developed by coupling the pressure drop model and the seepage model. Then, the determining factors, key nodes of the maximum value, and derivation and propagation laws of the dynamic underbalanced pressure are revealed. An influence study focusing on an ultra-deep well is conducted. The obtained results indicated that the dynamic underbalanced pressure describes a fast drop in the annulus fluid pressure caused by the fluid flow among the annulus, the perforating guns, and the perforation tunnels since the pressures in the perforating guns and the perforation tunnels are lower than the annulus pressure after the perforation detonation. The annulus fluid pressure can slowly recover and increase due to the fluid flow between the screens and the annulus and the fluid flow between the reservoir rock and the wellbore. The main form of the wellbore failure caused by the dynamic underbalanced pressure is the casing collapse deformation. Therefore, changing perforating conditions, wellbore conditions, and reservoir rock conditions can increase the maximum for the dynamic underbalanced pressure to improve the success rate of the combined technology. The research presented in this paper can provide a new understanding and prediction method of the dynamic underbalanced pressure.

Transient kinetic analysis of the standard SCR reduction half cycle on Cu-SSZ-13 catalysts: Roles of temperature, hydrothermal aging and H2O feed content

Transient Response Methods (TRM) are applied to investigate the reduction half-cycle (RHC) of the Standard SCR reaction over a state-of-the-art Cu-SSZ-13 catalyst at temperatures ranging from 135 to 350°C. We assess the effects of hydrothermal aging (HTA), water feed content and temperature on the reaction redox chemistry. By testing three samples with different aging levels, we reveal the CuII reduction rate to be unaffected by HTA in the whole T-range under investigation. Kinetic analysis assesses the inhibiting role of water on the RHC, consistent with literature. As long as enough NH3 is stored on the catalyst surface, reduction transients are accurately predicted assuming a second-order dependence of the reduction rate on the copper(II) fraction, also at temperatures exceeding 200°C. Remarkably, under wet conditions the low-T CuII reduction mechanism remains the same at least up to 300°C.

3D modelling of ground magnetic-source airborne TEM data for anisotropic media based on the rational Krylov method

With the rapid development of the transient electromagnetic method (TEM), the numerical simulations of three-dimensional anisotropic media have garnered attention. This study used the rational Krylov method to realise the three-dimensional modelling of the ground magnetic-source airborne transient electromagnetic data for arbitrary anisotropic media. First, based on Maxwell's equations, a time-domain electromagnetic equation was derived for anisotropic media. The finite difference method was employed to discretise the equation, and the electromagnetic field was then expressed as the product of an exponential matrix and a vector related to the source. Subsequently, the rational Arnoldi algorithm was adopted to construct the rational Krylov subspace orthogonal basis vectors, and the electromagnetic fields at different times were obtained based on a model reduction strategy. The accuracy of the proposed algorithm was verified through the comparisons of its results with those of other software packages. We calculated the response for different anisotropic parameters of the typical three-dimensional anisotropic models, and analysed the influence of the anisotropic media on the ground magnetic-source airborne transient electromagnetic data. The proposed three-dimensional forward TEM algorithm provides important technical support for identifying the anisotropic geological bodies and lays the foundation for further inversion research.

Transient frequency response test and measurement error prediction of DCTV based on adaptive inertial weight improved ACO

AbstractA temporary frequency response test and measurement error prediction method of direct current voltage transformer (DCTV) based on artificial intelligence (AI) is proposed. Firstly, the frequency characteristic of direct current (DC) side voltage of DCTV is analyzed. On this basis, a DCTV transient Frequency Response testing method based on transient alternating current (AC) & DC superposition was developed. Then, the method of voltage sudden change and phase correction is used to achieve transient process DCTV response time testing. Finally, the ant colony optimization (ACO) algorithm was improved by combining an adaptive inertia weight improvement strategy, achieving accurate prediction of the Measurement Error of DCTV. The proposed AI based DCTV transient Frequency Response testing and Measurement Error prediction method were compared and analyzed with the other three methods through simulation experiments. Compared to the other three comparison methods, the maximum transformation error in the evaluation indicators of mean squared error (MSE), root mean squared error (RMSE), and mean absolute error (MAE) decreased by 0.006, 0.0119, and 0.0085, respectively, while the maximum phase error decreased by 0.2794, 0.3004, and 0.2823, respectively.

Enhanced quasi-three-dimensional transient simulation technique incorporating component volume effects for aero engine

Current transient analysis predominantly relies on zero-dimensional/one-dimensional tools, proficient at capturing aerothermodynamic variations across critical engine stations but insufficient for analyzing the internal flow field evolution during transients. Addressing this gap, the study presents an enhanced quasi-three dimensional (quasi-3D) transient simulation technique that integrates component volume effects, offering a significant leap from the preceding quasi-3D transient simulation method based on quasi-steady assumption. By embedding the component volume effects on density, momentum, and energy within the physical temporal dimension of the Navier-Stokes equations, the refined quasi-3D transient model achieves a closer representation of physical phenomena. Validation against a single-shaft turbofan engine’s experimental data confirms the model’s accuracy. Average errors for key performance indicators, including shaft speed, thrust, mass flow rate, and critical component exit temperature and pressure, remain below 0.41%, 5.69%, 2.55%, 3.18% and 0.67%, respectively. Crucially, the model exposes a discernible temporal lag in the compressor outlet pressure and temperature response due to volume effects—previously unquantified in quasi-3D transient simulations. And further exploration of the meridional flow field emphasizes the consequential role of volumes in transient flow field evolution. Incorporating volume effects within quasi-3D transient simulations enhances engine modeling and is pivotal for precise transient analysis in engine design and optimization.

The frequency analytical method for regional power systems considering the emergency frequency control

With the continuous construction of high-voltage direct-current (HVDC) transmission projects, higher requirements are placed on the frequency security of the regional power systems. In order to ensure the frequency security of regional power systems, this paper proposes a transient frequency analytical method considering the emergency frequency control (EFC). Firstly, the aggregated system frequency response (SFR) model is constructed by reducing the order of the generator governor equation, the EFC equation, and the load model equation. Then, based on the aggregation model, the analytical solution of the transient frequency nadir of the regional power system is derived. The model can quickly and accurately calculate the transient frequency nadir of the regional power system considering EFC after the HVDC block fault. Finally, based on an actual regional power system model, the accuracy and applicability of the proposed method under HVDC block fault are verified.

Research and Simulation of Partial Discharge Phenomenon Sensing Technology for Substation Inspection Robots

Abstract This paper thoroughly investigates the phenomenon of partial discharge in substations and proposes a set of simulation experimental apparatus to replicate various types of partial discharge defects in switchgear. By modifying the switchgear, incorporating models for different types of partial discharge, and equipping various standard partial discharge sensors, accurate simulations of phenomena such as point discharge, suspended discharge, gap discharge, and particle discharge are achieved. Through this system, the effectiveness of different detection methods, such as ultrasonic, high-frequency, and transient voltage methods, is validated for various defect models. Furthermore, utilizing an indoor inspection robot guide rail, the paper simulates the environment of partial discharge in substations, enabling the validation of the robot’s partial discharge detection performance and typical patterns under different conditions. This work is dedicated to providing situational awareness technology for partial discharge phenomenon detection for substation inspection robots, so that the inspection robot can detect various abnormal partial discharge phenomena in transformers or other substation equipment through micro sensors to promote the stable operation of the substation.

A comparison of static and rotordynamic characteristics for two types of liquid annular seals with and without helical grooved stator

Less leakage is a benefit of parallel grooved liquid seals (labyrinth seals). But researches show that the liquid seal with parallel grooves on the rotor harms the rotor stability. The seal with helical grooves on stator performs well in terms of rotordynamics, and its leakage is sensitive to the rotating speed. To make use of the advantages of both seals and improve seal stability, based on the Smooth-stator/Parallel Grooved-rotor (SPG) liquid seal, a Helical Grooved-stator/Parallel Grooved-rotor (HGPG) liquid seal is designed. To evaluate two liquid seals’ leakage, rotordynamic characteristics and drag power loss, a transient computational fluid dynamics-based method is employed. This method is based on the multi-frequency elliptical-orbit rotor whirling model and the mesh deformation technique. The published experimental data of the leakage and rotordynamic force coefficients for an SPG liquid seal are used to validate the accuracy and dependability of the current method. Seal leakage and force coefficients are presented and compared for the SPG liquid seal and the HGPG liquid seal at various pressure drops. The influences of parallel groove depth on the leakage and rotordynamic properties for the HGPG liquid seals at two rotational speeds (2000, 6000 r/min) are analyzed. The numerical findings demonstrate that the novel HGPG liquid seal has a lower leakage flow rate (by ∼22.3%) than the traditional SPG liquid seal. There is an optimal parallel groove depth that minimizes leakage. The presented novel HGPG liquid seal significantly improves rotordynamic stability, due to the similar effective stiffness and the obviously larger positive effective damping. Reducing parallel groove depth can increase the positive effective damping. In terms of leakage and rotordynamic characteristics, the novel HGPG liquid seal is a better seal design for liquid turbomachinery.

ECONOMIC AND MATHEMATICAL MODELING OF THE RENEWABLE ENERGY DEVELOPMENT IN UKRAINE

Objective. The objective of the article is to apply economic and mathematical models for the analysis, modeling and forecasting of the development of renewable energy in Ukraine, assessment of country's renewable energy sources potential. Methods. The following methods are used to model and forecast the development of renewable energy in Ukraine: correlational and actocorrelational analysis (to identify the relationship between macroeconomic indicators of Ukraine and the development of renewable energy in the country); three-factor autoregressive and multiplicative forecasting and five-factor regression forecasting (for forecasting indicators of renewable energy of Ukraine); the basics of the mathematical apparatus for the analysis of transient processes, the methodology of determining the coefficients of impulse-transient and transient functions, methods of building differential models, Laplace transformation and obtaining transient functions, the basics of the mathematics of imaginary quantities, methods of obtaining the amplitude-phase and frequency characteristics of the object under study, methods of analysis stability of controlled dynamic processes (to present the Total renewable energy indicator in the form of a dynamic transient process and model its future states by obtaining a second-order differential equation with a simple input, a complex transfer function and by determining the amplitude-phase frequency characteristic and constructing a Nyquist frequency hodograph, which showed the stability of the studied process to external disturbances). Results. Forecasting the installed capacities and production volumes of renewable energy is an important task for the effective development of this industry. A new approach to forecasting is proposed, combining autoregressive and multivariate regression models and dynamic modeling. The development of the capacity of renewable energy is considered as a dynamic transient process, which is represented by a complex transfer function with its real frequency characteristic and its imaginary frequency characteristic, which are used to construct the Nyquist hodograph, which shows the stability of the researched process of development of the capacity of renewable energy from investment investments. According to the results of modeling based on the transient process method, a model of changes in the volume of installed capacities of renewable energy of Ukraine depending on the volume of investments in renewable energy is obtained in the form of a differential equation, and it is found out that this process is stable, that is, even with temporary restrictions or reductions of such investment over time it will return to a stable growing trend. The proposed method of dynamic modeling makes it possible to obtain more accurate and reliable forecasts of the development of renewable energy capacity, compared to traditional methods. This method can be used to make informed decisions regarding the development of solar energy and investment in this industry in Ukraine.

PagMYB128 regulates anthocyanin biosynthesis by directly activating PagMYB116 in Populus alba × Populus glandulosa

MYB transcription factors regulate the biosynthesis of anthocyanin, a crucial flavonoid in plants. In this research, we identified PagMYB128 as a new activator of anthocyanin production in the hybrid poplar (Populus alba × Populus glandulosa). The PagMYB128 protein was located in the nucleus and has higher expression level in stems. Dominant repression of PagMYB128 led to a reduction of anthocyanin content in the petiole and stems. Overexpression of PagMYB128 resulted in the opposite phenotype. We found that PagMYB128 promoted anthocyanin biosynthesis by activating PagCHS1, PagF3’5’H1, PagDFR2, and PagANR1, which encoded the essential enzymes of anthocyanin biosynthesis. Further, PagMYB116 was identified as a potential direct target of PagMYB128 by ChIP-seq. Through ChIP-qPCR, Y1H, and Split-LUC assays, it was demonstrated that PagMYB128 could directly interact with the MYB-site located in the promoter region of PagMYB116, thereby exerting control over the transcription of PagMYB116. Moreover, through the poplar transient transformation method, we found that PagMYB116 could activate anthocyanin biosynthetic enzyme genes PagF3’5’H1 and PagANR1, through direct binding to their promoters. These findings indicate that the PagMYB128-MYB116 module for transcriptional regulation plays a crucial part in the synthesis of anthocyanin, enriching our comprehension of the networks responsible for regulating anthocyanin transcription.

Effect of phase change materials on thermophysical properties of asphalt mixtures and snow melting performance

The increased Thermal Conductivity and Thermal Diffusion Coefficient of asphalt mixtures means that the material is able to conduct and disperse heat more efficiently. In winter, this property can be used to accelerate the melting process of snow and ice, reducing the risk of road sliding due to snow and ice build-up. In this study, the thermal conductivity, thermal diffusion coefficient, and specific heat capacity of phase change asphalt (PCA) mixtures were investigated using a transient flat-plate heat source method. Analysis was performed with a thermal constant analyzer, a custom-designed thermal conductivity experiment, and a differential scanning calorimeter. Additionally, a phase change asphalt mixture thermoregulation rutting plate was prepared to evaluate its thermoregulation and snow and ice resistance under actual winter and indoor conditions. Results indicated that the thermal conductivity and thermal diffusion coefficient of PCA mixtures were superior to those of conventional asphalt mixtures. Notably, the specific heat capacity of these mixtures increased significantly within the phase change temperature range. The integration of phase change materials enhanced heat transfer within the asphalt mixtures, allowing for a delayed cooling process when temperatures decreased, with the maximum cooling range observed to be 2.8°C. Snow melting tests confirmed the early snowfall efficacy of the phase change asphalt mixture rutting plate, effectively achieving minimal snow accumulation and demonstrating the capability of 'melting light snow.'

The Effect of Transverse Sinus Stenosis Caused by Arachnoid Granulation on Patients with Venous Pulsatile Tinnitus: A Multiphysics Interaction Simulation Investigation.

This study aimed to investigate the effect of the transverse sinus (TS) stenosis (TSS) position caused by arachnoid granulation on patients with venous pulsatile tinnitus (VPT) and to further identify the types of TSS that are of therapeutic significance for patients. Multiphysics interaction models of six patients with moderate TSS caused by arachnoid granulation and virtual stent placement in TSS were reconstructed, including three patients with TSS located in the middle segment of the TS (group 1) and three patients with TTS in the middle and proximal involvement segment of the TS (group 2). The transient multiphysics interaction simulation method was applied to elucidate the differences in biomechanical and acoustic parameters between the two groups. The results revealed that the blood flow pattern at the TS and sigmoid sinus junction was significantly changed depending on the stenosis position. Preoperative patients had increased blood flow in the TSS region and TSS downstream where the blood flow impacted the vessel wall. In group 1, the postoperative blood flow pattern, average wall pressure, vessel wall vibration, and sound pressure level of the three patients were comparable to the preoperative state. However, the postoperative blood flow velocity decreased in group 2. The postoperative average wall pressure, vessel wall vibration, and sound pressure level of the three patients were significantly improved compared with the preoperative state. Intravascular intervention therapy should be considered for patients with moderate TSS caused by arachnoid granulations in the middle and proximal involvement segment of the TS. TSS might not be considered the cause of VPT symptoms in patients with moderate TSS caused by arachnoid granulation in the middle segment of the TS.

Application of C(t)-integral Solutions in Extending ASME BPVC Section Xi Division 2 Code Case N-934 for Transient Creep Crack Growth

Abstract Creep crack growth is a phenomenon which arises in damaged metallics structures under combined primary and secondary loads in the creep regime. The High Temperature Flaw Evaluation Code Committee of ASME BPVC is evaluating methods in extending Code Case N-934 to capture transient creep crack growth. This paper provides context and basic examples on the selected approach to transient creep crack growth methods based on the C(t)-integral. The basis for selected C(t)-integral solution as analytical method is established, including an overview of its derivation. Practical assessments of a crack growing under creep conditions in a realistic component are conducted to illustrate the analytical approach. Additional considerations in the application of the analytical methods and limitations are discussed.

Axial Coordinated Manganese(III) Porphyrin/Tetraazaporphyrin - 4-(10-phenylanthracen-9-yl)Pyridine Dyads: Self-Assembly, Structure and Spectral Properties in Ground and Excited States.

Self-assembly of new donor-acceptor systems based on (5,10,15,20-tetraphenylporphinato)manganese(III)/(5,10,15,20-tetra-4-tert-butylphenylporphinato)manganese(III)/(octakis(4-tert-butylphenyl)tetraazaporphinato)manganese(III) acetate ((AcO)MnTPP/(AcO)MnTBPP/(AcO)MnTAP) and 4-(10-phenylanthracen-9-yl)pyridine (PyAn) was studied using fluorescence spectroscopy and mass spectrometry. It was found that the coordination complexes of 1 : 1 composition (dyads) are formed in toluene. The spectral properties, the chemical structures and redox behavior of the dyads were described using 1H NMR, IR, ESR spectroscopy and cyclic voltammetry, respectively. The dynamic processes and the characteristics in the excited state of the dyads were obtained using the femtosecond transient absorption spectroscopy method. Density functional theory (DFT), time-dependent DFT methods were used to elucidate the dyad electronic structures and to establish the differences in their frontier molecular orbitals. The analysis of the lambda parameter and the distance of hole-pair interaction was indicated more favorable charge transfer between the macrocycle and the axial PyAn fragment in (AcO)(PyAn)MnTAP. The calculated values of the zero-field splitting parameters D and E/D, together with the g tensors of the lowest spin-orbit state for (AcO)MnTPP and (AcO)(PyAn)MnTPP were obtained using the combination of DFT and Multireference Perturbation Theory (CASSCF/NEVPT2) simulations. The data obtained develop the fundamental basis in the field of photovoltaics and show the prospects for the study of molecular systems of this class.

A simple and efficient gene functional analysis method for studying the growth and development of peach seedlings.

Stable genetic transformation of peach [Prunus persica (L.) Batsch] still faces many technical challenges, and existing transient expression methods are limited by tissue type or developmental stage, making it difficult to conduct functional analysis of genes regulating shoot growth. To overcome this dilemma, we developed a three-step method for efficient analysis of gene functions during peach seedling growth and development. This method resulted in transformation frequencies ranging from 48 to 87%, depending on the gene. From transformation of germinating seeds to phenotyping of young saplings took just 1.5months and can be carried out any time of year. To test the applicability of this method, the function of three tree architecture-related genes, namely PpPDS, PpMAX4, and PpWEEP, and two lateral root-related genes, PpIAA14-1 and -2, were confirmed. Since functional redundancy can challenge gene functional analyses, tests were undertaken with the growth-repressor DELLA, which has three homologous genes, PpDGYLA (DG), PpDELLA1 (D1), and -2 (D2), in peach that are functionally redundant. Silencing using a triple-target vector (TRV2-DG-D1-D2) resulted in transgenic plants taller than those carrying just TRV2-DG or TRV2. Simultaneously silencing the three DELLA genes also attenuated the stature of two dwarf genotypes, 'FHSXT' and 'HSX', which normally accumulate DELLA proteins. Our study provides a method for the functional analysis of genes in peach and can be used for the study of root, stem, and leaf development. We believe this method can be replicated in other woody plants.

Three-Dimensional Numerical Simulation of Pumped Storage Unit No-Load operation with PID control before Grid-Connected

Abstract The “S” characteristic curve has been a persistent challenge for low-head grid connection of pump-turbine units. The stability of the no-load operation before grid connection directly determines the success of grid connection. In order to accurately simulate the characteristics of the no-load operation before grid connection, this paper applies a PID speed controller model to the full flow system 3D simulation of the pumped storage unit, using user-defined functions and dynamic grid technology. The calculation results were compared with the conventional transient calculation method and field test data, which verified the reliability of the calculation method and solved the shortcomings of the conventional transient calculation method, especially in terms of pressure pulsation. The application of this speed controller model provides a new method for studying the instability of the “S” characteristic curve in pump-turbine units.

Solid-phase transient soldering method based on Au/Ni–W multilayer thin-film-modified copper-based structures

The copper crystal cone-shaped micro-nanostructure is used as the substrate, and the Ni–W alloy layer and Au nanolayer are plated sequentially. Instantaneous soldering with lead-free solder is realized under ultrasonic assistance at room temperature. This solves the residual stress and thermal damage caused by high melting point lead-free solder on thin chips and thermal components, and ensures the safety and reliability of electronic components. Copper-based microstructures are deposited by electrochemical methods. An amorphous Ni–W alloy layer with a thickness of 180 nm is deposited on the Cu-based microstructures by adjusting the atomic ratio of the plating solution. The Ni–W layer is further coated with a 50 nm Au layer to prevent oxidation. Solid-phase transient soldering is realized by combining the Au/Ni–W multilayer thin-film-modified Cu substructures with commercial solder (SAC305) for a holding time of 3 s at a soldering pressure of 10,000 gf (20 MPa) while ultrasonically assisted. The soldered samples are aged at 180 °C for 10 min, 30 min, and 60 min, respectively. Copper substructures with different surface modifications are subjected to destructive shear experiments with solder balls. Scanning electron microscope and X-ray fluorescence thickness gauge are used to study the microstructure, intermetallic compound (IMC) composition thickness and properties of the soldered interface and section. The cone height of the Cu-based structure is 2–4 μm, and the diameter of the bottom is 800 nm–1200 nm, which has a sharp tip and an excellent L/D ratio. The interface between the Au/Ni–W modified Cu substructure and the solder ball is almost free of holes. The average shear strength at the soldering interface is about 43.06 MPa. The fracture surface after the shear experiment basically occurs inside the solder ball matrix, which belongs to the pure toughness fracture. The interface between the Au/Ni–W-modified Cu-based structure and the solder ball is subjected to long aging treatment at 180 °C. The soldering interface showed a “bright layer”. New phases are generated on the solder side above the “bright layer”, while no new phases appear on the Cu substructure side below the “bright layer”. The copper-based microstructure is inserted into the inside of the solder ball to form an inlay and produce mechanical interlocking. Au/Ni–W alloy modification layer can effectively improve the surface hardness of copper-based structures. This creates a large hardness difference with soft solder and enables the formation of fewer holes in the insertion solder. Amorphous Ni–W alloys are prone to form dense oxide films during ultrasonication. The Au film modification prevents oxide generation and increases the average shear strength of the soldering interface. The Ni–W alloy layer retards the interdiffusion between Cu–Sn, blocks the excessive growth of Cu–Sn IMCs, and reduces the reliability problems caused by interface failure.

Genome-scale identification, expression and evolution analysis of B-box members in Dendrobium huoshanense

B-box (BBX) proteins have been recognized as vital determinants in plant development, morphogenesis, and adaptive responses to a myriad of environmental stresses. These zinc-finger proteins play a pivotal role in various biological processes. Their influence spans photomorphogenesis, the regulation of flowering, and imparting resilience to a wide array of challenges, encompassing both biotic and abiotic factors. Chromosome localization, gene structure and conserved motifs, phylogenetic analysis, collinearity analysis, expression profiling, fluorescence quantitative analysis, and tobacco transient transformation methods were used for functional localization and expression pattern analysis of the DhBBX gene. A total of 23 DhBBX members were identified from Dendrobium huoshanense. Subsequent phylogenetic evaluations effectively segregated these genes into five discrete evolutionary subsets. The predictions of subcellular localizations revealed that all these proteins were localized in the nucleus. The genetic composition and patterns showed that the majority of these genes consisted of several exons, with a few variations that could be attributed to transposon insertion. A comprehensive analysis using qRT-PCR was conducted to unravel the expression patterns of these genes in D. huoshanense, with a specific concentration on their responses to various hormone treatments and cold stress. Subcellular localization reveals that DhBBX21 and DhBBX9 are located in the nucleus. Our results provide a deep comprehension of the complex regulatory mechanisms of BBXs in response to various environmental and hormonal stimuli. These discoveries encourage further detailed and focused investigations into the operational dynamics of the BBX gene family in a wider range of plant species.

Robust soybean leaf agroinfiltration.

A robust agroinfiltration-mediated transient gene expression method for soybean leaves was developed. Plant genotype, developmental stage and leaf age, surfactant, and Agrobacterium culture conditions are important for successful agroinfiltration. Agroinfiltration of Nicotiana benthamiana has emerged as a workhorse transient assay for plant biotechnology and synthetic biology to test the performance of gene constructs in dicot leaves. While effective, it is nonetheless often desirable to assay transgene constructs directly in crop species. To that end, we innovated a substantially robust agroinfiltration method for Glycine max (soybean), the most widely grown dicot crop plant in the world. Several factors were found to be relevant to successful soybean leaf agroinfiltration, including genotype, surfactant, developmental stage, and Agrobacterium strain and culture medium. Our optimized protocol involved a multi-step Agrobacterium culturing process with appropriate expression vectors, Silwet L-77 as the surfactant, selection of fully expanded leaves in the VC or V1 stage of growth, and 5min of vacuum at -85kPa followed by a dark incubation period before plants were returned to normal growth conditions. Using this method, young soybean leaves of two lines-V17-0799DT, and TN16-5004-were high expressors for GUS, two co-expressed fluorescent protein genes, and the RUBY reporter product, betalain. This work not only represents a new research tool for soybean biotechnology, but also indicates critical parameters for guiding agroinfiltration optimization for other crop species.We speculate that leaf developmental stage might be the most critical factor for successful agroinfiltration.

Modeling transient temperature coupled pressure behaviour for waterflooding well with induced fractures: Semi-analytical model, numerical model, and case studies

Waterflooding is a widely used technique for developing unconventional reservoirs. Long-term waterflooding can lead to the formation of multiple induced fractures. Accurately tracking the location, extent, and dynamic behavior of these induced fractures is crucial. To enhance monitoring capabilities, data obtained from distributed temperature sensors are used to track temperature variations within the wellbore and at the bottom of the well. Combining temperature and pressure inversion techniques helps mitigate the issue of multiple solutions while offering a more comprehensive characterization of multi-induced fractures from various perspectives. In this study, this work presents a model for temperature coupled pressure (TCP) transient analysis. Additionally, this work utilizes COMSOL Multiphysics software to develop and solve a dynamic wellbore temperature (DWT) model. This work proposes a workflow that describes the dynamic behaviour of multi-induced fractures. During model development and solution, this work employs both pressure and temperature transient analysis methods, examining time-dependent non-linear pressure drop and heat transfer variations. Our results reveal that induced fractures exhibit convective heat exchange with the wellbore, resulting in significantly higher temperatures at the induced fracture locations compared to other wellbore locations. By analyzing temperature-wellbore location curves, this work can accurately identify the locations of induced fractures. The TCP model successfully extracts characteristic parameters of induced fractures, while the DWT model allows for monitoring the locations and heights of these fractures. By combining the workflows of both models, this work achieves a comprehensive characterization of the physical properties of induced fractures. In conclusion, this advancement empowers engineers to manage the development of such fractures effectively, thereby averting potential adverse effects on production well performance and preventing the need for well abandonment.