Transient Interference Research Articles

Mutual interferences in frequency-modulated continuous-wave (FMCW) LiDARs

This paper investigates mutual interferences between FMCW LiDARs both experimentally and theoretically. We show that the FMCW LiDARs have mutual interference problems as those of the FMCW radars. The transient interference degrades the signal-to-noise ratio and the in-band interference generates ghost images. Finally, we discuss their impacts to the autonomous vehicles, and mitigation methods.

P1134Cardiac pacemakers and transient electromagnetic interferences during radiotherapy courses: a multicentre, real-time, in-vitro evaluation

Abstract Funding Acknowledgements None Background The effects of high dose oncologic radiotherapy (RT) on cardiac pacemakers (PMs), at even less than 6 MV power, are unpredictable, depending on multiple factors. Normal PM function may be impaired during direct exposure, due to electromagnetic interferences (EMIs). Potentially life-threatening malfunctions may occur, expecially in PM-dependent patients, and both manufacturers and guidelines discourage direct exposure. Purpose To evaluate transient EMI-related PM malfunctions during direct exposure to doses up to 10 Gy during radiotherapy course, 17 wireless-telemetry-enabled PMs with sufficient residual battery charge for the purpose of the study (at least 4 months to elective replacement indicator, E.R.I.) were evaluated in three different centres. Methods All PMs underwent baseline interrogation. Single chamber devices were programmed in the VVI/40 mode while dual or triple chamber devices were programmed in the DDD/40 mode. To avoid the "run-away" phenomenon during exposure rate-adaptive function was disabled. A centering computed tomography was performed to build the corresponding treatment plan and the PMs were blinded randomized to receive either 2, 5 or 10 Gy exposure by a 6 MV linear accelerator in a homemade water phantom (600 Um/min). The effective dose received by the PMs was randomly assessed by an in-vivo dosimetry. During RT course, the devices were observed in a real-time session using manufacturer specific equipment, and PM function (pacing and sensing, programmed parameters) was recorder by a video camera in the bunker throughout the entire radiation exposure. Results During RT course, 13 PMs (76.5%) recorded not clinically relevant minor transient EMIs, and no atrial and/or ventricular oversensing nor base-rate-pacing inhibition were observed. Only 4 PMs (23.5%) reported neither transient malfunction nor minor EMIs, withstanding direct radiation exposure. Transient EMI-related malfunctions were observed regardless of either 2, 5 or 10 Gy exposure. Conclusions Minor, not clinically relevant EMI-related interferences were observed in most of the PMs during direct exposure. Nevertheless, to avoid potentially life-threatening PM malfunctions, magnet application on the PM pocket site or reprogramming are still suggested in PM-dependent (high risk) patients ongoing even low energy RT exposure.

Lexical Access in Naming and Reading: Spatiotemporal Localization of Semantic Facilitation and Interference Using MEG

Naming an object involves quick retrieval of a target word from long-term memory. Research using the semantic interference paradigm has shown that objects take longer to name when they are preceded by primes in the same semantic category. This has been interpreted as reflecting either competition during lexical selection or as an interference effect at a later, postlexical level. Since the behavioral finding has been a core argument for the existence of competition during lexical selection in naming, understanding its processing level is important for models of language production. We used MEG to determine the spatiotemporal localization of the interference effect. We also compared its neural signature to the effect of semantic relatedness in reading, in which relatedness is expected to speed up behavioral responses and reduce activity in the left superior temporal cortex at around 200–300 ms. This is exactly what we found. However, in naming, we observed a more complex pattern for our semantically related targets. First, the angular gyrus showed a facilitory pattern at 300–400 ms, likely reflecting aspects of lexical access. This was followed by a broadly distributed and sustained interference pattern that lasted until articulatory stages. More transient interference effects were also observed at 395–485 ms in the left STG and at ∼100–200 ms before articulation in the parietal cortex. Thus, our findings suggest that the semantic interference effect originates from both early and late sources, which may explain its varying localizations in previous literature.

RNA Interference-Based Screen Reveals Concerted Functions of MEKK2 and CRCK3 in Plant Cell Death Regulation.

A wide variety of intrinsic and extrinsic cues lead to cell death with unclear mechanisms. The infertility of some death mutants often hurdles the classical suppressor screens for death regulators. We have developed a transient RNA interference (RNAi)-based screen using a virus-induced gene silencing approach to understand diverse cell death pathways in Arabidopsis (Arabidopsis thaliana). One death pathway is due to the depletion of a MAP kinase (MAPK) cascade, consisting of MAPK kinase kinase 1 (MEKK1), MKK1/2, and MPK4, which depends on a nucleotide-binding site Leu-rich repeat (NLR) protein SUMM2. Silencing of MEKK1 by virus-induced gene silencing resembles the mekk1 mutant with autoimmunity and defense activation. The RNAi-based screen toward Arabidopsis T-DNA insertion lines identified SUMM2, MEKK2, and Calmodulin-binding receptor-like cytoplasmic kinase 3 (CRCK3) to be vital regulators of RNAi MEKK1-induced cell death, consistent with the reports of their requirement in the mekk1-mkk1/2-mpk4 death pathway. Similar with MEKK2, overexpression of CRCK3 caused dosage- and SUMM2-dependent cell death, and the transcripts of CRCK3 were up-regulated in mekk1, mkk1/2, and mpk4 MEKK2-induced cell death depends on CRCK3. Interestingly, CRCK3-induced cell death also depends on MEKK2, consistent with the biochemical data that MEKK2 complexes with CRCK3. Furthermore, the kinase activity of CRCK3 is essential, whereas the kinase activity of MEKK2 is dispensable, for triggering cell death. Our studies suggest that MEKK2 and CRCK3 exert concerted functions in the control of NLR SUMM2 activation and MEKK2 may play a structural role, rather than function as a kinase, in regulating CRCK3 protein stability.

Discovery and characterization of tannase genes in plants: roles in hydrolysis of tannins.

Plant tannins, including condensed tannins (CTs) and hydrolyzable tannins (HTs), are widely distributed in the plant kingdom. To date, tannase (TA) - is a type of tannin acyl-hydrolase hydrolyzing HTs, CT monomer gallates and depsides - has been reported in microbes only. Whether plants express TA remains unknown. Herein, we report plant TA genes. A native Camellia sinensis TA (CsTA) is identified from leaves. Six TAs are cloned from tea, strawberry (Fragaria×ananassa, Fa) and four other crops. Biochemical analysis shows that the native CsTA and six recombinant TAs hydrolyze tannin compounds, depsides and phenolic glycosides. Transcriptional and metabolic analyses reveal that the expression of CsTA is oppositely associated with the accumulation of galloylated catechins. Moreover, the transient overexpression and RNA interference of FaTA are positively associated with the accumulation of ellagitannins in strawberry fruit. Phylogenetic analysis across different kingdoms shows that 29 plant TA homologs are clustered as a plant-specific TA clade in class I carboxylesterases. Further analysis across the angiosperms reveals that these TA genes are dispersed in tannin-rich plants, which share a single phylogenetic origin c. 120 million yr ago. Plant TA is discovered for the first time in the plant kingdom and is shown to be valuable to improve tannin compositions in plants.

FvbHLH9 Functions as a Positive Regulator of Anthocyanin Biosynthesis by Forming a HY5-bHLH9 Transcription Complex in Strawberry Fruits.

Anthocyanin accumulation is transcriptionally regulated by the MYB-bHLH-WD40 complex. Light is indispensable for anthocyanin accumulation, and light-inducible MYB and HY5 were considered to promote anthocyanin accumulation in many fruits. Whether and how light-inducible bHLH transcription factor and HY5 regulate anthocyanin synthesis in strawberry is unknown. In this study, we identified a bHLH transcription factor, FvbHLH9, which was induced by light as well as FvHY5, and found that, similar to FvHY5, the transient overexpression and interference FvbHLH9 in strawberry fruits can promote and decrease anthocyanin accumulation, respectively, indicating FvbHLH9 functions as a positive regulator of anthocyanin biosynthesis. Furthermore, we confirmed that both FvHY5 and FvbHLH9 specifically bind to the promoter region of some key enzyme genes, including FvDFR, and the expression of FvDFR was activated through the heterodimer formation between FvHY5 and FvbHLH9. Finally, we confirmed that FvbHLH9-promoted anthocyanin accumulation is dependent on HY5-bHLH heterodimerisation in Arabidopsis. Our findings provide insights into a mechanism involving the synergistic regulation of light-dependent coloration and anthocyanin biosynthesis via a HY5-bHLH heterodimer formed by the interaction of FvHY5 and FvbHLH9 in strawberry fruits.

Assessment of Transient EMI Impact on LTE Communications Using EVM & PAPR

The brief contact losses between the pantograph and the catenary in high-speed trains create transient interferences, which extend to the frequency bands used by telecommunication systems. This article presents a study of the impact of these transient wideband interferences on LTE communications between the train and a base station. In this study, we set up an experimental test bench, which establishes LTE communication between a communication tester and a USB/LTE dongle in the presence of transient interferences. We analysed the impact of these interferences on the error vector magnitude (EVM) of the received LTE signals and we observed that the EVM evolution does not explain certain communication interruptions. Indeed, interruptions can occur for EVM values significantly inferior to the standard limits. To understand the relationship between the interference characteristics and the communication interruption, we studied the peak to average power ratio (PAPR). Indeed, the PAPR is well suited to analyse transient interferences because it evolves with the amplitude but also with the repetition of the transient. Then, the PAPR measurement can provide a diagnostic assistance to analyse whether along certain railway lines, pantograph-catenary interferences are the cause of the LTE interruptions.

Closed-Loop Gate Drive for Single-Ended Forward Converter to Reduce Conducted EMI

Forward DC/DC converters are widely used in low and medium power circuits that are widely used in the aerospace and navigation fields. Power converters are usually sources of electromagnetic interference (EMI), which is due to their higher voltage and current transients. The traditional method is to add expensive filters on the primary side or the secondary side, but its disadvantages are high cost and poor adjustability. Based on the principle that the greater the number of derivations of voltage transients, the lower the high frequency electromagnetic interference contained in this voltage, a signal containing small high-frequency noise that is suitable for the hardware circuit as a reference signal is selected, and a closed-loop gate drive method is used in this paper, which attenuate the conducted EMI noise in the input bus of the forward DC/DC converter. The reference signal shapes the output signal to achieve the purpose of suppressing electromagnetic interference through the closed loop circuit. The advantages of this method are making the output highly adjustable, reducing hardware size and weight and lower cost compared with filter method. Compared with the method of controlling current to reduce electromagnetic interference, the proposed method is easier to implement. Firstly, the relationship between the derivative order of voltage transient and electromagnetic interference is introduced. Secondly, the experimental principle of single-ended forward circuit controlled by closed loop is introduced. Simulation and experimental results show that the proposed method can achieve voltage shaping and suppression of voltage overshoot effectively. Compared with the traditional hard-switching control method, the proposed method can make the high-frequency components of the drain-source voltage of the primary-side MOS transistor and the secondary-side output voltage have a greater attenuation.

An End-to-end Transient Recognition Method for VSC-HVDC Based on Deep Belief Network

Lightning is one of the most common transient interferences on overhead transmission lines of high-voltage direct current (HVDC) systems. Accurate and effective recognition of faults and disturbances caused by lightning strokes is crucial in transient protections such as traveling wave protection. Traditional recognition methods which adopt feature extraction and classification models rely heavily on the performance of signal processing and practical operation experiences. Misjudgments occur due to the poor generalization performance of recognition models. To improve the recognition rates and reliability of transient protection, this paper proposes a transient recognition method based on the deep belief network. The normalized line-mode components of transient currents on HVDC transmission lines are analyzed by a deep belief network which is properly designed. The feature learning process of the deep belief network can discover the inherent characteristics and improve recognition accuracy. Simulations are carried out to verify the effectiveness of the proposed method. Results demonstrate that the proposed method performs well in various scenarios and shows higher potential in practical applications than traditional machine learning based ones.

An Improved Behavior Model for IGBT Modules Driven by Datasheet and Measurement

This article describes an improved behavior model for IGBT modules. The new steady-state characteristic model of the IGBT with a correction function was built to improve the saturation current for different gate-emitter voltages. Two important nonlinear capacitances were described by two continuous functions to solve the convergence problem in the circuit simulation. The parasitic parameters generated by the package were extracted by the impedance measurement. All the parameters of the model are derived from datasheet and measurement. The improved model was implemented by MAST language in SABER circuit simulation. A double-pulse test was applied to verify the improved model. Compared with the conventional model for transient waveforms, switching losses, and electromagnetic interference (EMI), the improved model shows a better agreement with experiments. The satisfactory results indicate that the improved model can offer a simple, fast, and generic modeling approach.

Virus-virus interactions impact the population dynamics of influenza and the common cold.

The human respiratory tract hosts a diverse community of cocirculating viruses that are responsible for acute respiratory infections. This shared niche provides the opportunity for virus-virus interactions which have the potential to affect individual infection risks and in turn influence dynamics of infection at population scales. However, quantitative evidence for interactions has lacked suitable data and appropriate analytical tools. Here, we expose and quantify interactions among respiratory viruses using bespoke analyses of infection time series at the population scale and coinfections at the individual host scale. We analyzed diagnostic data from 44,230 cases of respiratory illness that were tested for 11 taxonomically broad groups of respiratory viruses over 9 y. Key to our analyses was accounting for alternative drivers of correlated infection frequency, such as age and seasonal dependencies in infection risk, allowing us to obtain strong support for the existence of negative interactions between influenza and noninfluenza viruses and positive interactions among noninfluenza viruses. In mathematical simulations that mimic 2-pathogen dynamics, we show that transient immune-mediated interference can cause a relatively ubiquitous common cold-like virus to diminish during peak activity of a seasonal virus, supporting the potential role of innate immunity in driving the asynchronous circulation of influenza A and rhinovirus. These findings have important implications for understanding the linked epidemiological dynamics of viral respiratory infections, an important step towards improved accuracy of disease forecasting models and evaluation of disease control interventions.

Design of Heading Fault-Tolerant System for Underwater Vehicles Based on Double-Criterion Fault Detection Method

This paper proposes a heading fault tolerance scheme for operation-level underwater robots subject to external interference. The scheme is based on a double-criterion fault detection method using a redundant structure of a dual electronic compass. First, two subexpansion Kalman filters are set up to fuse data with an inertial attitude measurement system. Then, fault detection can effectively identify the fault sensor and fault source. Finally, a fault-tolerant algorithm is used to isolate and alarm the faulty sensor. The program can effectively detect the constant magnetic field interference, change the magnetic field interference and small transient magnetic field interference, and conduct fault tolerance control in time to ensure the heading accuracy of the system. Test verification shows that the system is practical and effective.

2410Oncologic radiotherapy-induced implantable cardioverter-defibrillator malfunctions: a real-time, in-vitro evaluation

Abstract Background Direct exposure of implantable cardioverter-defibrillators (ICDs) during radiotherapy is still considered not recommended, or even unsafe, by manufacturers and guidelines. The effects of photon beams on ICDs are unpredictable, depending on multiple factors, and malfunctions may occur during exposure. Purpose To evaluate transient ICD malfunctions by direct exposure to doses up to 10 Gy during low-energy radiotherapy, 33 contemporary wireless-enabled ICDs, with at least 4 months to elective replacement indicator (E.R.I.) were evaluated in a realtime in-vitro session. Methods All ICDs had baseline interrogation. Single chamber ICDs were programmed in the VVI/40 mode and dual or triple chamber ICDs were programmed in the DDD/40 mode. Rate response function and antitachycardia therapies were disabled, with the ventricular tachycardia (VT)/ventricular fibrillation (VF) detection windows still active. A centering computed tomography was performed to build the corresponding treatment plan and the ICDs were blinded randomized to receive either 2, 5 or 10 Gy exposure by a low photon-energy linear accelerator (6MV) in a homemade water phantom (600 MU/min). The effective dose received by the ICDs was assessed by an in-vivo dosimetry. During radiotherapy, the devices were observed in a real-time session using manufacturer specific programmer, and ICD function (pacing, sensing, programmed parameters, detection) was recorder by the video camera in the bunker throughout the entire photon exposure. All ICDs had an interrogation session immediately after exposure. Results During radiotherapy course, almost all ICDs (90.9%) recorded major or minor transient electromagnetic interferences. On detail, 13 ICDs (39.4%) reported atrial and/or ventricular oversensing, with base-rate-pacing inhibition and VT/VF detection. 16 ICDs (48.5%) recorded non clinically relevant noise, and no detections were observed. Only 4 ICDs (12.1%) reported neither transient malfunction nor minor noise, withstanding direct radiation exposure. At immediate post-exposure interrogation, the ICDs that recorded major real-time malfunctions had VT/VF detections stored in the device memory. In none of the ICDs spontaneous changes in parameter settings were reported. Malfunctions occurred regardless of either 2, 5 or 10 Gy photon beam exposure. Conclusions Transient electromagnetic interferences were observed in most of the contemporary ICDs during radiotherapy course, regardeless of photon dose. To avoid potentially life-threatening ICD malfunctions such as pacing inhibition or inappropriate shock delivery, magnet application on the pocket site or reprogramming devices in the asynchronous mode are still suggested in ICD patients ongoing even low energy radiotherapy exposure. Acknowledgement/Funding None

Transient Voltage Analysis for Dynamic Positioning Closed Bus Electrical System on Ship in Laboratory Scale

Ships equipped with a dynamic positioning system has a more number of thuster than ships that are not equipped with a dynamic positioning system. The addition of this thruster resulted in a significant increase in electrical loads so that the power plant will be added to the vessel. Dynamic positioning is a system used to support offshore vessel work consisting of a collection of equipment and control systems that work simultaneously to maintain the position of a vessel in accordance with which has been input into the system. Installation of the dynamic positioning system on the vessel affects the electrical system of the vessel. In the operation of electrical system on the vessel found the disturbances that arise one of them is transient interference that causes the change of voltage and frequency value in a short time. A Transient voltage occurs within a few seconds where the system conditions are unstable. The research was conducted in a laboratory scale by modeled a series of vessels that have the system with existing laboratory equipment where transient interference occurred due to the starting motor so that it is done Analysis of the magnitude of the voltage and frequency in the specified scenario as well as finding a solution to correct emerging interference that exceeds the standard. In the experiments made some configuration changes and obtained the result that the configuration of Split Plant 1 generator thruster has good system stability capable of achieving a stable condition with a loading of 50%. As for the loading of 60% stability of the system is unstable and can be improved again with the addition of capacitor.

Technical note: Interferences of volatile organic compounds (VOCs) on methane concentration measurements

Abstract. Studies that quantify plant methane (CH4) emission rely on the accurate measurement of small changes in the mixing ratio of CH4 that coincide with much larger changes in the mixing ratio of volatile organic compounds (VOCs). Here, we assessed whether 11 commonly occurring VOCs (e.g. methanol, α- and β-pinene, Δ3-carene) interfered with the quantitation of CH4 by five laser-absorption spectroscopy and Fourier-transformed infrared spectroscopy (FTIR) based CH4 analysers, and quantified the interference of seven compounds on three instruments. Our results showed minimal interference with laser-based analysers and underlined the importance of identifying and compensating for interferences with FTIR instruments. When VOCs were not included in the spectral library, they exerted a strong bias on FTIR-based instruments (64–1800 ppbv apparent CH4 ppmv−1 VOC). Minor (0.7–126 ppbv ppmv−1) interference with FTIR-based measurements were also detected when the spectrum of the interfering VOC was included in the library. In contrast, we detected only minor (<20 ppbv ppmv−1) and transient (< 1 min) VOC interferences on laser-absorption spectroscopy-based analysers. Overall, our results demonstrate that VOC interferences have only minor effects on CH4 flux measurements in soil chambers, but may severely impact stem and shoot flux measurements. Laser-absorption-based instruments are better suited for quantifying CH4 fluxes from plant leaves and stems than FTIR-based instruments; however, significant interferences in shoot chamber measurements could not be excluded for any of the tested instruments. Our results furthermore showed that FTIR can precisely quantify VOC mixing ratios and could therefore provide a method complementary to proton-transfer-reaction mass spectrometry (PTR-MS).

Molecular Characterization and Functional Study of Insulin-Like Androgenic Gland Hormone Gene in the Red Swamp Crayfish, Procambarus clarkii.

The androgenic gland (AG) is a male-specific endocrine organ that controls the primary and secondary sexual characteristics in male crustaceans. More evidence indicates that the insulin-like androgenic gland hormone gene (IAG) is the key male sexual differentiation factor, particularly the application of RNA interference (RNAi) technology on IAG. In this study, the full-length cDNA of IAG (termed PcIAG) was isolated from the red swamp crayfish, Procambarus clarkii. Tissue distribution analysis showed that in addition to its expression in the AG of male P. clarkii, PcIAG was widely expressed in female tissues and other male tissues. The PcIAG protein was detected in the reproductive and nervous systems of adult male P. clarkii. Additionally, RNAi results showed that the PcIAG expression could be silenced efficiently, and the male sperm maturation and release possibly present a transient adverse interference at lower doses (0.1 μg/g and 1 μg/g) of PcIAG–dsRNA (PcIAG double-stranded RNA). Dramatically, the expression level of PcIAG increased sharply shortly after the injection of higher doses (5 μg/g and 10 μg/g) of PcIAG–dsRNA, which might accelerate the maturation and release of sperm. Moreover, the expression of PcSxl (P. clarkii Sex-lethal) was detected by Quantitative Real-Time PCR (qPCR) after the injection of PcIAG–dsRNA to explore whether the PcIAG gene regulates the PcSxl gene, and we found that the PcIAG did not directly regulate PcSxl in P. clarkii. The study could help accelerate the progress of PcIAG functional research and provide a useful reference for the single-sex selective breeding of P. clarkii.

High-harmonic spectroscopy of transient two-center interference calculated with time-dependent density-functional theory.

We demonstrate high-harmonic spectroscopy in many-electron molecules using time-dependent density-functional theory. We show that a weak attosecond-pulse-train ionization seed that is properly synchronized with the strong driving mid-infrared laser field can produce experimentally relevant high-harmonic generation (HHG) signals, from which we extract both the spectral amplitude and the target-specific phase (group delay). We also show that further processing of the HHG signal can be used to achieve molecular-frame resolution, i.e., to resolve the contributions from rescattering on different sides of an oriented molecule. In this framework, we investigate transient two-center interference in CO2 and OCS, and how subcycle polarization effects shape the oriented/aligned angle-resolved spectra.

Time‐domain low‐frequency non‐periodic transient EMI measurement system

In this study, a time-domain low-frequency non-periodic transient electromagnetic interference (EMI) measurement system is presented. By the use of an antenna, an anti-aliasing filter, and a digital oscilloscope, the radiated emissions measurement specified by electromagnetic compatibility standard MIL-STD-461 is achieved. In the first stage, the antenna receives the radiated emissions of the device under test and the time domain output voltage is acquired in the oscilloscope. Then, to fulfil the spectrum measurement as accurate as an EMI receiver, a spectrum calculating methodology is carried out. Optimised inverse fast Fourier transform procedure for spectrum estimation in the proposed methodology is implemented to reduce the calculation efforts. The proposed EMI measurement technique required only a single time-domain acquisition and no repeatedly continuous scanning was imposed on the frequency measurement of the EMI test receivers. Due to this benefit, scan times are decreased by several orders of magnitude in comparison with a traditional EMI receiver.

Tandem 13-Lipoxygenase Genes in a Cluster Confers Yellow-Green Leaf in Cucumber.

Some lipoxygenase (LOX) isoenzymes can co-oxidize carotenoids. Carotenoids are collectors of light energy for photosynthesis and can protect plants from reactive oxygen species and coloration. This study isolated the cucumber (Cucumis sativus L.) yellow-green leaf mutant (ygl1), which had yellow-green leaves with decreased chlorophyll synthesis, increased relative carotenoid content, and delayed chloroplast development. Genetic analysis demonstrated that the phenotype of ygl1 was caused by a recessive mutation in a nuclear gene. The bulked segregants were resequenced, and the candidate ygl1 locus identified was mapped to the 9.2 kb region of the chromosome 4. Sequence analysis revealed that ygl1 encodes the tandem 13-LOX genes in a cluster. Four missense mutations were found in four tandem 13-LOX genes (Csa4M286960, Csa4M287550, Csa4M288070, and Csa4M288080) in the ygl1 mutant, and the four 13-LOX genes showed high similarity with one another. The transient RNA interference and virus-induced gene silencing of these genes simultaneously resulted in yellow-green leaves with a reduced amount of chloroplasts and increased relative carotenoid content, which were observed in the ygl1 mutant. This evidence supported the non-synonymous SNPs (Single Nucleotide Polymorphism) in the four tandem 13-LOX genes as being the causative mutation for the yellow-green leaves. Furthermore, this study provides a new allele for breeding cucumbers with yellow-green leaves and serves as an additional resource for studying carotenoid biosynthesis.

Ubiquitin Extension Protein UEP1 Modulates Cell Death and Resistance to Various Pathogens in Tobacco.

Ubiquitin (Ub) extension proteins (UEPs) are fusion proteins of a Ub at the N terminus to a ribosomal protein. They are the main source of Ub and the only source of extension ribosomal protein. Although important roles of the Ub-26S proteasome system in various biological processes have been well established, direct evidence for the role of UEP genes in plant defense is rarely reported. In this study, we cloned a Ub-S27a-type UEP gene (NbUEP1) from Nicotiana benthamiana and demonstrated its function in cell death and disease resistance. Virus-induced gene silencing of NbUEP1 led to intensive cell death, culminating in whole-seedling withering. Transient RNA interference (RNAi) of NbUEP1 caused strong cell death in infiltrated areas, while stable NbUEP1-RNAi tobacco plants constitutively formed necrotic lesions in leaves. NbUEP1-RNAi plants exhibited increased resistance to the oomycete Pythium aphanidermatum and viruses Tobacco mosaic virus and Cucumber mosaic virus while displaying decreased resistance to the nematode Meloidogyne incognita compared with non-RNAi control plants. Transcription profiling analysis indicated that jasmonate and ethylene pathways, lipid metabolism, copper amine oxidase-mediated active species generation, glycine-rich proteins, vacuolar processing enzyme- and RD21-mediated cell death and defense regulation, and autophagy might be associated with NbUEP1-mediated cell death and resistance. Our results provided evidence for the important roles of plant UEPs in modulating plant cell death and disease resistance.