PURPOSES : In this study, the effects of when median exclusive bus lanes were applied to Daejeon trunk road (Wolpyeng crossway~Seodaejeon crossway, 6.3km) and (Daeduck Bridge 4~Kyeryong 4, 2.6km) by Microscopic Simulation VISSIM (5.0) was studied. The median exclusive bus lanes are one of the measures of transportation system manage techniques that can especially improve the efficiency of public transportation facilities. METHODS : According to the analysis of VISSIM on the Gyerong mainroad and Daedeok mainroad, when the median exclusive bus lanes were applied unlike when the roadside bus-only lanes were applied, the average travel speed of vehicles decreased but the average delay time and travel time increased. This arised from the changes in the geometric structure of the road which occurred the reduction of vehicle lane in the center of the road. RESULTS : In the case of bus, on the other hand, the average travel speed increased but the average delay time and travel time decreased. This is because the problems such as illegal parking and stopping, secondary road in out vehicle, and conflict of intersection right turn that roadside bus-only lanes occurred was solved. CONCLUSIONS : Although the introduction of median exclusive bus lanes will have a negative effect on general traffic flow due to the aggravation of travel, decrease of passenger car usage will lead to decrease of traffic volume. Therefore, smooth vehicle travel is expected.