Traditional Plant Research Articles

Exploring the Landscape of Media Campaigns That Encourage or Discourage Sustainable Diet Transitions for Americans, 1917–2023: A Systematic Scoping Review

United States (U.S.) and global experts recommend that populations reduce red and processed meat (RPM) intake and transition to plant-rich, sustainable diets to support human and planetary health. A systematic scoping review was conducted to identify the landscape of media campaigns that promote plant-rich dietary patterns, traditional plant proteins, and novel plant-based meat alternatives (PBMA) and that encourage or discourage RPM products to Americans. Of 8321 records screened from four electronic databases, 103 records were included, along with 62 records from gray literature sources. Across 84 media campaigns (1917–2023) identified, corporate marketing campaigns (58.6%) were most prevalent compared to public information (13.8%), corporate sustainability (12.6%), countermarketing (5.7%), social marketing (4.6%), and public policy (4.6%) campaigns. Findings indicate that long-running corporate RPM campaigns, many with U.S. government oversight, dominated the landscape for decades, running alongside traditional plant protein campaigns. Novel PBMA campaigns emerged in the past decade. Many civil society campaigns promoted plant-rich dietary patterns, but few utilized social norm or behavior change theory, and only the Meatless Monday campaign was evaluated. The U.S. government, academia, businesses, and civil society should commit more resources to and evaluate the impact of media campaigns that support a sustainable diet transition for Americans, restrict and regulate the use of misinformation in media campaigns, and prioritize support for plant-based proteins and plant-rich dietary patterns.

Exploration of antidiabetic potential of traditional ethno-medicinal plant Viscum cruciatum Sieber ex Boiss. (Loranthaceae) from Rawalakot district, Poonch, Azad Jammu and Kashmir, Pakistan

Exploration of antidiabetic potential of traditional ethno-medicinal plant Viscum cruciatum Sieber ex Boiss. (Loranthaceae) from Rawalakot district, Poonch, Azad Jammu and Kashmir, Pakistan

Ethnobotanical insights into the traditional food plants of the Baiku Yao community: a study of cultural significance, utilization, and conservation

BackgroundThe Baiku Yao, primarily residing in Guangxi and Guizhou provinces of China, is a distinctive branch of the Yao ethnic group, known for their profound cultural preservation and unique ethnobotanical knowledge. This study investigates the Baiku Yao community’s utilization of traditional food plants, focusing on the relationship between their dietary practices and the local biodiversity within their mountainous living environment. It aims to illuminate the cultural significance and survival strategies embedded in their ethnobotanical knowledge, highlighting the potential for sustainable living and biodiversity conservation.MethodsThrough ethnobotanical surveys, key informant interviews, and quantitative analysis techniques such as the cultural food significance index (CFSI) and relative frequency of citations (RFC), this research systematically documents the diversity and cultural importance of edible plants in the Baiku Yao community. The study assesses how these plants contribute to the community’s diet, traditional medicine, and overall cultural practices.ResultsA total of 195 traditional edible plants were documented, belonging to 142 genera and 68 families, with a significant concentration in certain families such as Asteraceae, Rosaceae, and Fabaceae. The Baiku Yao diet prominently features herbaceous plants, with wild (103 species) and cultivated (89 species) varieties as diverse food sources. They utilize various plant parts, particularly fruits and leaves, for multiple purposes, including nutrition, medicine, and fodder. Their processing techniques, from raw to fermented, showcase a rich culinary tradition and emphasize a holistic use of plants for enhancing diet and health in a concise overview. The RFC and CFSI analyses reveal a deep cultural reliance on a variety of plant species, with a notable emphasis on vegetables, fruits, spices, and medicinal herbs. Specific plants like Zingiber officinale, Zea mays, and Oryza sativa were highlighted for their high cultural significance. The study also uncovers the multifunctional use of these plants, not only as food but also for medicinal purposes, fodder, and other cultural applications, reflecting the Baiku Yao’s profound ecological wisdom and their harmonious coexistence with nature.ConclusionThe findings emphasize the rich ethnobotanical knowledge possessed by the Baiku Yao, underscoring the importance of documenting, safeguarding, and transmitting this invaluable traditional knowledge. This study contributes to a deeper understanding of cultural heritage and biodiversity conservation, advocating for concerted efforts to protect such traditional practices against the threats of modernization and cultural erosion.

Review on Mercury Control during Co-Firing Coal and Biomass under O2/CO2 Atmosphere

Combining biomass co-firing with oxy-fuel combustion is a promising Bioenergy with Carbon Capture and Storage (BECCS) technology. It has the potential to achieve a large-scale reduction in carbon emissions from traditional power plants, making it a powerful tool for addressing global climate change. However, mercury in the fuel can be released into the flue gas during combustion, posing a significant threat to the environment and human health. More importantly, mercury can also cause the fracture of metal equipment via amalgamation, which is a major risk for the system. Therefore, compared to conventional coal-fired power plants, the requirements for the mercury concentration in BECCS systems are much stricter. This article reviews the latest progress in mercury control under oxy-fuel biomass co-firing conditions, clarifies the impact of biomass co-firing on mercury species transformation, reveals the influence mechanisms of various flue gas components on elemental mercury oxidation under oxy-fuel combustion conditions, evaluates the advantages and disadvantages of various mercury removal methods, and finally provides an outlook for mercury control in BECCS systems. Research shows that after biomass co-firing, the concentrations of chlorine and alkali metals in the flue gas increase, which is beneficial for homogeneous and heterogeneous mercury oxidation. The changes in the particulate matter content could affect the transformation of gaseous mercury to particulate mercury. The high concentrations of CO2 and H2O in oxy-fuel flue gas inhibit mercury oxidation, while the effects of NOx and SO2 are dual-sided. Higher concentrations of fly ash in oxy-fuel flue gas are conducive to the removal of Hg0. Additionally, under oxy-fuel conditions, CO2 and metal ions such as Fe2+ can inhibit the re-emission of mercury in WFGD systems. The development of efficient adsorbents and catalysts is the key to achieving deep mercury removal. Fully utilizing the advantages of chlorine, alkali metals, and CO2 in oxy-fuel biomass co-firing flue gas will be the future focus of deep mercury removal from BECCS systems.

The Antioxidant Dendrobium officinale Polysaccharide Modulates Host Metabolism and Gut Microbiota to Alleviate High-Fat Diet-Induced Atherosclerosis in ApoE-/- Mice.

The discovery of traditional plants' medicinal and nutritional properties has opened up new avenues for developing pharmaceutical and dietary strategies to prevent atherosclerosis. However, the effect of the antioxidant Dendrobium officinale polysaccharide (DOP) on atherosclerosis is still not elucidated. This study aims to investigate the inhibitory effect and the potential mechanism of DOP on high-fat diet-induced atherosclerosis in Apolipoprotein E knockout (ApoE-/-) mice. The identification of DOP was measured by high-performance gel permeation chromatography (HPLC) and Fourier transform infrared spectroscopy (FTIR). We used high-fat diet (HFD)-induced atherosclerosis in ApoE-/- mice as an animal model. In the DOP intervention stage, the DOP group was treated by gavage with 200 μL of 200 mg/kg DOP at regular times each day and continued for eight weeks. We detected changes in serum lipid profiles, inflammatory factors, anti-inflammatory factors, and antioxidant capacity to investigate the effect of the DOP on host metabolism. We also determined microbial composition using 16S rRNA gene sequencing to investigate whether the DOP could improve the structure of the gut microbiota in atherosclerotic mice. DOP effectively inhibited histopathological deterioration in atherosclerotic mice and significantly reduced serum lipid levels, inflammatory factors, and malondialdehyde (F/B) production. Additionally, the levels of anti-inflammatory factors and the activity of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), were significantly increased after DOP intervention. Furthermore, we found that DOP restructures the gut microbiota composition by decreasing the Firmicutes/Bacteroidota (F/B) ratio. The Spearman's correlation analysis indicated that serum lipid profiles, antioxidant activity, and pro-/anti-inflammatory factors were associated with Firmicutes, Bacteroidota, Allobaculum, and Coriobacteriaceae_UCG-002. This study suggests that DOP has the potential to be developed as a food prebiotic for the treatment of atherosclerosis in the future.

Optimized ridge-furrow technology with biochar amendment for alfalfa yield enhancement and soil erosion reduction based on a structural equation model on sloping land

Water scarcity and water-related soil erosion are severely exacerbated by inappropriate human activities and global climate change. Hence, to find a suitable technology to mitigate drought and soil erosion, three consecutive field experiments were conducted to explore the impact of ridge-furrow cropping with biochar amendment on soil water storage, runoff, sediment yield, soil nutrient losses, alfalfa (Medicago sativa L) fodder yield, crop water productivity (WPc), and economic benefit from 2019 to 2021. This experiment was conducted in a split-plot design, taking biochar amendment patterns (no biochar amendment and biochar amendment at a rate of 3× 104 kg ha−1) as a main plot and ridge-furrow technologies (traditional planting, open-ridging, and tied-ridging) as a split-plot. The combination of biochar amendment with ridge-furrow technology, especially tied-ridging technology, increased soil water storage, and captured runoff, sediment, and related soil nutrient losses, consequently increasing alfalfa fodder yield, WPc, and income. During this study, compared to traditional planting, open-ridging depicted an increase in soil water storage by a range of 9.8–39.6 mm, an alfalfa fodder yield boost ranging from 9.8% to 38.6%, and a WPc increase ranging from 0.1 to 16.5 kg ha−1 mm−1. On the other hand, tied-ridging showed greater improvements with soil water storage increasing by 29.1–65.1 mm, alfalfa fodder yield growing by 11.6–44.4%, and WPc advancing by 0.9–17.5 kg ha−1 mm−1. The mean decrease in runoff, sediment, and nutrients (total nitrogen, total phosphorus, and organic matter) loss for open-ridging was 17.9%-37.7%, 46.4%-75.5%, and 40.4%-75.3%, respectively, while for tied-ridging, it was 22.3%-55.5%, 62.1%-87.6%, and 49.0%-87.3%, respectively. Compared to no biochar amendment, soil water storage, alfalfa fodder yield, and WPc for biochar amendment increased by 9.1%-20.4%, 5.8%-52.7%, and 4.6–7.8 kg ha−1 mm−1, respectively, while runoff, sediment, and nutrients loss for biochar amendment decreased by 32.2%-40.9%, 25.5%-55.5%, and 35.9%-53.3%, respectively. Structural equation modeling analysis indicated that the significant direct effect of biochar amendment and ridge-furrow technology on WPc was 0.20 and 0.62, respectively, whereas the significant direct effect of runoff and actual crop evapotranspiration on alfalfa fodder yield was −0.40 and 0.94, respectively. Tied-ridge cropping combined with biochar addition was a highly suggested approach for addressing soil erosion and enhancing alfalfa fodder yields in the Loess Plateau in China. This technology could mitigate soil water scarcity and soil erosion, and give farmers the confidence to invest in this technology in dryland regions.

Speed Breeding in Cereal Crops: Accelerating Genetic Improvement for Rapid Agricultural Advancement

The global demand for food is rising due to population growth, climate change, and evolving consumer preferences. Traditional plant breeding programs typically require a decade or more to develop and release new crop varieties. Speed breeding is a cutting-edge technique designed to expedite genetic improvement by significantly reducing the seed-to-seed cycle. This review explores the principles, applications, and potential of this method in accelerating agricultural advancement. Originating from NASA's experiments for food production in space, the approach has evolved into a highly efficient strategy for speeding up breeding cycles in cereal crops. By manipulating light, photoperiodic regimes, temperature, and humidity within controlled environments, it is possible to achieve up to six generations of photo-insensitive crops and two to three generations of other crops per year. This rapid generation turnover provides a unique opportunity for accelerated genetic mapping, trait stacking, and enhanced genomic selection. Speed breeding complements modern breeding technologies such as genome editing, high-throughput genotyping, and CRISPR, facilitating quicker development of crop varieties with improved traits. Its applications extend to boosting transgenic pipelines and understanding critical physiological traits in crops. Furthermore, it aligns with breeding methods like single plant selection and single seed descent, offering a more efficient path to desirable outcomes. However, challenges remain, including the need for specialized infrastructure, the impact of genotypic variations, and potential stress responses due to accelerated conditions. Despite these hurdles, speed breeding represents a promising tool in the quest for food security and resilient agricultural systems. This review examines its potential to transform plant breeding, reduce cultivar development times, and contribute to rapid agricultural advancement in a changing world.

Variations in Cold Resistance and Contents of Bioactive Compounds among Dendrobium officinale Kimura et Migo Strains.

Dendrobium officinale is a valuable traditional Chinese herbal plant that is both medicinal and edible. However, the yield of wild Dendrobium officinale is limited. Adverse stress affects the growth, development, and yield of plants, among which low temperature is the primary limiting factor for introducing Dendrobium officinale to high-latitude areas and expanding the planting area. Therefore, this study aims to explore the variations in growth ability, cold resistance, and contents of bioactive compounds among different Dendrobium officinale strains. Four strains of Dendrobium officinale were selected as experimental materials and were subjected to low-temperature stress (4 °C). The agronomic traits, physiological indices, as well as the expressions of cold resistance-related genes (HSP70, DcPP2C5, DoCDPK1, and DoCDPK6) in the roots and leaves of Dendrobium officinale, were determined. The contents of bioactive compounds, including polysaccharides, flavonoids, and phenols were also measured. Compared with the other strains, Xianju had the highest seed germination and transplantation-related survival rates. Under low-temperature stress, Xianju exhibited the strongest cold resistance ability, as revealed by the changes in water contents, chlorophyll levels, electrical conductivities, enzyme activities, and expressions of the cold resistance-related genes. Additionally, the polysaccharide content of Xianju increased the most, while the stem flavonoid and leaf phenol contents were elevated in all four strains under cold treatment. Therefore, selecting excellent performing strains is expected to expand the planting area, improve the yield, and increase the economic benefits of Dendrobium officinale in high latitude areas with lower temperatures.

A deep learning approach for Maize Lethal Necrosis and Maize Streak Virus disease detection

Maize is an important crop cultivated in Sub-Saharan Africa, essential for food security. However, its cultivation faces significant challenges due to debilitating diseases such as Maize Lethal Necrosis (MLN) and Maize Streak Virus (MSV), which can lead to severe yield losses. Traditional plant disease diagnosis methods are often time-consuming and prone to errors, necessitating more efficient approaches. This study explores the application of deep learning, specifically Convolutional Neural Networks (CNNs), in the automatic detection and classification of maize diseases. We investigate six architectures: Basic CNN, EfficientNet V2 B0 and B1, LeNet-5, VGG-16, and ResNet50, using a dataset of 15344 images comprising MSV, MLN, and healthy maize leaves. Additionally, We performed hyperparameter tuning to improve the performance of the models and Gradient-weighted Class Activation Mapping (Grad-CAM) for model interpretability. Our results show that the EfficientNet V2 B0 model demonstrated an accuracy of 99.99% in distinguishing between healthy and disease-infected plants. The results of this study contribute to the advancement of AI applications in agriculture, particularly in diagnosing maize diseases within Sub-Saharan Africa.

Scales of plant stewardship in the precontact Pacific Northwest, USA

Numerous oral histories and substantial ethnographic evidence illustrate how plant species, communities, and even landscapes were extensively managed and cared for by ancestral communities in the Pacific Northwest. Camas ( Camassia spp.) is one such cultural keystone plant, common from the Pacific Ocean to the Rocky Mountains, with numerous records describing its role as a staple food for many Northwest peoples. Supporting deep time archeological evidence for such management or stewardship practices, however, has remained elusive. In this paper we analyze archived collections of archeological camas bulbs from 11 sites across the Willamette Valley, Oregon to demonstrate people began preparing camas within earth ovens by approximately 8000 calendar years before present and deliberately harvesting sexually mature camas plants circa 3500 calendar years before present. We compare these findings with climatological, palynological, and fire history reconstructions to discuss stewardship strategies for camas and associated plant communities through time at the population, community, and landscape levels. These findings confirm and expand upon Indigenous knowledges as well as offer time-tested methods for cultural keystone conservationists seeking to revitalize traditional plant stewardship practices throughout this region and beyond. This “camas case study” also offers another example of a human-plant symbiotic relationship, expanding our knowledge of plant food pathways, processes, and mutualisms.

Extraction and Analysis of Medicinal Property in Secondary Metabolites Obtained from Selected Plant Species

Medical plants have been used from thousands of years to treat and prevent many diseases. Wockhardt global school Consist various plants variety and for this study three medicinal plants were selected and these plants are Tridax procumbiens (Tantani), Ficus racemosa (Umbar), Nyctanthes abortiristis (Parijat). The primary aim of this study was to extract and identify the presence of phytochemicals and to determine their medicinal uses for human beings. From Vedic era the traditional plant medicines has been considered as a primary healthcare for human beings. In the given study the presence of flavonoids, alkaloids, N-Containing chemicals were determined by using phytochemical extraction method. The chemicals were identified based upon various qualitative tests. The selected plants consist glycosides, alkaloids, saponins, flavonoids and carbohydrates. This phytochemicals are responsible for anti-inflammatory properties hence the selected medicinal plants can be used for inflammation, bone pain, joint pain, swelling, and common cough etc. Keywords: Medicinal plants, secondary metabolites, phytochemicals, flavonoids, alkaloids, leaf extract.

Optimizing removal of antiretroviral drugs from tertiary wastewater using chlorination and AI-based prediction with response surface methodology

Chemical and pharmaceutical chemicals found in water sources create substantial risks to human health and the environment. The presence of pharmaceutical contaminants in water can cause antibiotic resistance development, toxicity to aquatic organisms, and endocrine disruption. Hence, the elimination of chemicals and other contaminants from wastewater prior to its release is a burgeoning concern in the domains of engineering and science. The use of treatment technologies in wastewater treatment plants can remove pharmaceutical contaminants through the oxidation process. However, many traditional wastewater treatment plants lack the advanced monitoring tools required to detect low concentrations of pharmaceuticals. Without the ability to detect these compounds, it's challenging to treat them effectively. The goal of this study was to use Response Surface Methodology (RSM) and Artificial Neural Networks (ANN) algorithms to model and improve how Nevirapine and Efavirenz break down in different chlorination conditions. The RSM analysis revealed statistically significant models (F-values: Nevirapine, pH-t: 108.15, T-t: 76.55, ICC-t: 110.84), indicating a strong correlation between operational parameters (pH, temperature, and initial chlorine concentration) and degradation behavior. The ANN model accurately predicted the degradation of both Nevirapine and Efavirenz under various chlorination conditions, as confirmed by analyzing actual-predicted graphs, residual plots, and Mean Squared Error (MSE) values. The ANN model using ICC-t achieved the highest MOD value of 31.31 % for Nevirapine. The ANN model based on ICC-t yielded a maximum MOD value of 16.06 % for Efavirenz. These findings provide valuable insights into optimizing chlorination processes for better removal of these pharmaceutical contaminants from water.

Effect of Planting Media and Cultivation Method in the Offshoots Growth of Date Palm Phoenix Dactylifera L. Hillawi cv.

Objective: The study aimed to explore the effects of different planting medium on the growth of date palm Hillawi cv. offshoots. Methods: The experiment included two main factors: the first factor was planting media, consisting of three treatments: field soil, zeolite + field soil (in a 2:1 ratio), and zeolite + sand (in a 2:1 ratio). The second factor was cultivation method, comprising three methods: traditional planting, container planting (using pots), and planting in lined holes with a layer of agricultural polyethylene (nylon). Results: Planting medium significantly influenced the growth of the offshoots. Specifically, planting media with zeolite and sand exhibited significant improvements in leaf length, dry matter content, leaf area, root count, root length, and diameter compared to the control group. Conversely, the zeolite-field soil combination exhibited a marked increase in moisture content. Additionally, electrical conductivity (EC) within the planting media was notably higher in the control condition featuring only field soil. The study also found that the chosen cultivation method significantly influenced the growth of the offshoots. The lined holes method displayed significant advantages in leaf length, dry matter content, leaf area, root count, root length, root diameter, and moisture content within the planting media, compared to the traditional approach. Conversely, the traditional method resulted in higher EC within the planting media. Conclusions: The study reveals significant effects of cultivation medium, method, and their interaction on various growth parameters of date palm offshoots. This study recommends the lined-hole planting method and zeolite-infused medium for enhanced offshoot growth.

Microfiber prevalence and removal efficiency of textile effluent treatment plants in Bangladesh

Bangladesh is one of the hubs of the textile industry in the world; consequently, microfibers are an emerging threat to the aquatic ecosystem. Traditional effluent treatment plants (ETPs) might not be capable of removing most emerging pollutants like surfactants, dyes, and additives, including microfibers, and the textile industry may be a major source of microfiber pollution through products and ETPs. This study investigated the eleven woven, knit, and denim industries' ETP microfiber abundance and removal efficiency. The average (range) of microfibers found in influent, effluent, and sludge samples was 615.45 ± 377.52 particles/L (170−1460), 212.72 ± 80.14 particles/L (130−380), and 10545.45 ± 7989.54 particles/kg (4400−31000), respectively. The abundant shape was fiber, and most microfibres had sizes between 0 and 100 μm. Eight distinct colors of microfibers were found in the samples; black was the most abundant color among all the samples, followed by brown and blue. The studied ETPs showed a 23.52 % to 82.19 % microfiber removal rate, which is not satisfactory for minimizing pollution. The Fourier transform infrared (FTIR) analysis revealed that the main polymers in the samples were nylon, ethylene-vinyl acetate (EVA), polyethylene terephthalate (PETE), acrylonitrile butadiene styrene (ABS), cellulose acetate (CA), low-density polyethylene (LDPE or linear LDPE), and high-density polyethylene (HDPE). The contamination factor (CF) and pollution load index (PLI), which assess ecological risks, showed that samples were moderately to very highly contaminated by microfiber and could pose a threat to the aquatic ecosystem. The findings would help identify transformative challenges required for minimizing microfiber pollution from industrial sectors and improving ETP systems.

Development of A Robotic Maize Seed Planter

The automatic maize seed planter design to address the limitations of traditional planting techniques and reduce the lead time in agricultural processes and enhance the output. The research encompasses a comprehensive study of maize sowing techniques, challenges, and advancements in agricultural robotics. The literature review explores the limitations of traditional maize sowing techniques, such as inaccurate seed placement, labor intensiveness, and weather dependence. The construction process involves the integration of a chassis, wheels, sensors, microcontroller, and seed dispensing system. The software component utilizes a custom algorithm to control the movement and seed placement of the robot. Experimental testing and evaluation were conducted to assess the performance of the robot. The accuracy of seed placement was determined by analyzing the deviation from the desired spacing. The efficiency of the robot was measured by comparing the time taken for sowing with traditional methods. The results demonstrated that the automatic maize seed planter achieved high accuracy in seed placement with minimal deviation from the desired spacing. The results validate the effectiveness of the robotic system in addressing the limitations of traditional sowing techniques and demonstrate its potential to revolutionize the agriculture industry. The objectives are to develop a cost-effective, efficient, and accurate maize seed planter and implement the design in the fabrication. Recommendations for further work is real-time mapping and monitoring. Real-time mapping and monitoring of the field can help the robot navigate the field more efficiently and accurately.

赤峰市促进中蒙医药产业高质量发展策略研究

Chifeng is a traditional Chinese and Mongolian medicinal plant planting area in the north of China. It has the advantages of vast territory, rich kinds of medicinal materials and good quality of medicinal materials. It has a unique geographical position, great difference between the north and the south climate, and has the characteristics of a wide variety of wild plants and rich medicinal plant resources. As the dominant pharmaceutical industry in Chifeng, Mongolian medicine industry has the advantages of large policy support and abundant authentic medicinal materials, and has great development potential. In this paper, the author finds out the development dilemma of Mongolian medicine industry in Chifeng city through field investigation and visit of pharmaceutical enterprises, and focuses on solving the practical problems of high-quality development of pharmaceutical enterprises, so as to truly keep traditional medicine honest and innovative, so as to break the existing dilemma of the development of Chinese-Mongolian medicine industry in Chifeng city, play a better role of favorable policies, and promote the vigorous development of Chinese-Mongolian medicine industry in Chifeng region.

Orchid callus extract: a sustainable and natural active to promote hair growth

Background: Tissue culture is an important tool that can help to overcome many of the limitations associated with traditional plant cultivation methods. By adopting tissue culture techniques, it is possible to produce high-quality plant based raw materials in a more sustainable and environment friendly way. By cultivating calluses of Dendrobium spp. under controlled conditions, it is possible to obtain a consistent and high-quality supply of bioactive compounds that can be harvested and purified. This approach may also reduce the need for traditional harvesting methods that can damage natural habitats and ecosystems. Aims: Evaluating the efficacy of orchid callus extract as a sustainable active that can be deployed in the personal care industry as a possible hair growth promoting ingredients Methods: Six different callus extracts were prepared and tested on in vitro hair growth assays using goat and human hair follicles. Selected extracts were further validated on the proliferation profile of human hair follicle stem cells. Results: This study provides evidence that orchid callus extracts have a positive impact on stimulating the growth of hair follicles and improving the proliferation capacity of hair follicle stem cells. Conclusion: The findings of this study suggest that orchid callus extracts may have potential applications in hair care formulations. The use of sustainable and natural sources of bioactive compounds, such as orchid callus extract, may offer a more environment friendly alternative to traditional ingredients and may help to meet the growing demand for natural and sustainable personal care products.

U-Net with Coordinate Attention and VGGNet: A Grape Image Segmentation Algorithm Based on Fusion Pyramid Pooling and the Dual-Attention Mechanism

Currently, the classification of grapevine black rot disease relies on assessing the percentage of affected spots in the total area, with a primary focus on accurately segmenting these spots in images. Particularly challenging are cases in which lesion areas are small and boundaries are ill-defined, hampering precise segmentation. In our study, we introduce an enhanced U-Net network tailored for segmenting black rot spots on grape leaves. Leveraging VGG as the U-Net’s backbone, we strategically position the atrous spatial pyramid pooling (ASPP) module at the base of the U-Net to serve as a link between the encoder and decoder. Additionally, channel and spatial dual-attention modules are integrated into the decoder, alongside a feature pyramid network aimed at fusing diverse levels of feature maps to enhance the segmentation of diseased regions. Our model outperforms traditional plant disease semantic segmentation approaches like DeeplabV3+, U-Net, and PSPNet, achieving impressive pixel accuracy (PA) and mean intersection over union (MIoU) scores of 94.33% and 91.09%, respectively. Demonstrating strong performance across various levels of spot segmentation, our method showcases its efficacy in enhancing the segmentation accuracy of black rot spots on grapevines.

Metabolomic and transcriptomic reveal flavonoid biosynthesis and regulation mechanism in Phlomoides rotata from different habitats

Phlomoides rotata is a traditional medical plant at 3100–5200 m altitude in the Tibet Plateau. In this study, flavonoid metabolites were investigated in P. rotata from Henan County (HN), Guoluo County (GL), Yushu County (YS), and Chengduo County (CD) habitats in Qinghai. The level of kaempferol 3-neohesperidoside, sakuranetin, and biochanin A was high in HN. The content of limocitrin and isoquercetin was high in YS. The levels of ikarisoside A and chrysosplenol D in GL were high. Schaftoside, miquelianin, malvidin chloride, and glabrene in CD exhibited high levels. The results showed a significant correlation between 59 flavonoids and 29 DEGs. Eleven flavonoids increased with altitude. PAL2, UFGT6, COMT1, HCT2, 4CL4, and HCT3 genes were crucial in regulating flavonoid biosynthesis. Three enzymes CHS, 4CL, and UFGT, were crucial in regulating flavonoid biosynthesis. This study provided biological and chemical evidence for the different uses of various regional plants of P. rotata.

Life cycle assessment of methods for recycling retired ternary lithium batteries

Electric car sales in China are soaring. However, the battery life service is generally short, leaving room for dramatic waste related to different types of ternary lithium batteries. Since batteries contain substantial amounts of toxic substances, wrong recycling methods may significantly rise environmental pollution. Currently, China's Li-ion batteries recycling technologies are poor and research dealing with the environmental impact of recycling waste ternary lithium batteries by traditional hydrometallurgical technologies in China is not sufficient. In this paper, a life cycle assessment framework was carried out to analyze the environmental impacts of recycling LiNixCoyAl1-x-yO2 (NCA) and LiNixMnyCo1-x-yO2 (NMC) batteries using conventional hydrometallurgical techniques implemented in traditional Chinese plants. The freshwater ecotoxicity, human carcinogenicity and marine ecotoxicity of NCA batteries were higher than those of NMC batteries when the batteries were recovered by the traditional hydrometallurgical recovery method. The traditional recycling method would bring more toxicity to the environment and the traditional hydrometallurgical route is not suitable for recycling NCA-type ternary lithium batteries. Moreover, the recycling process of all three types of batteries displayed an impact on the environment with negative growth and the highest impact on the environment during the initial stage of recycling. Overall, new directions and technical updates for China's power battery recycling enterprises were provided, which can be useful for future planning.