Traditional Feed Research Articles

To what extent can the drilling process affect the drilling performance in Al‐mesh/GFRE hybrid composites?

AbstractA critical step in the bolted connection assembly of hybrid composites is drilling. Drilling effectiveness is influenced by the interaction between the structural constitution of the composite and the type of the drilling process. The performance of traditional (TD) and abrasive water jet (AWJ) drilling of glass fiber (GF) and hybrid glass fiber/aluminum‐mesh composites with two distinct Al patterns (AGF: with Al at the surface layer and GFA: with Al in the center) has been examined, with a focus on drilling performance. Delamination (), out of roundness (), Bearing strength (), and taper angle () are the quality criteria. The experimental studies were conducted under controlled parameters for TD (feed rate, speed and point angle) and for AWJ (water pressure, stand‐off distance and traverse speed) utilizing Taguchi L9 OA. TD method produced superior performance, with decrease of 10.75%, improved by 84.26%, and increased by 20.14%. While AWJD provides minimal thermal impacts, it produces increased and more variations in hole geometry owing to its sensitivity to specimen structures and processing parameters. The outcomes provided guidance for applications in aerospace and manufacturing, indicating that optimized TD is better suited for applications requiring high hole quality, and cost‐effectiveness.Highlights A comparative study of traditional and non‐traditional drilling processes. AWJD produces larger , less precise, and is affected by material properties. The TD showed compatibility for uses needing superior performance and quality.

Bridging the protein gap with single-cell protein use in aquafeeds

Blue foods from aquaculture are essential in bridging the protein gap to feed the human population in the future. However, for aquaculture production to be sustainable, production must be within planetary boundaries, and sourcing of sustainable raw materials is a key driver in sustainable production. This article explores the role of single-cell proteins (SCPs) derived from microorganisms in aquafeeds. Three main aspects are discussed: sustainability, scalability of fermentation technology, and fish performance. In addition, and through a comprehensive proof-of-concept trial with rainbow trout (Oncorhynchus mykiss), this article demonstrates SCP’s efficacy in replacing traditional feed ingredients without compromising fish growth and health. The trial’s findings demonstrate a high protein digestibility and a balanced amino acid profile, as well as health benefits measured through oxidative burst response. To date, commercial adoption of SCP has been hindered by high production costs and the need for substantial investments to scale fermentation technologies. However, the sustainability landscape is changing as large industry players openly commit to sustainability targets and realize that longer-term and investment thinking into the future is needed. In conclusion, SCP emerges as a promising avenue for sustainable aquafeeds, offering a solution to the protein supply challenge within planetary boundaries. In addition, in terms of environmental benefit, SCP shows clear advantages regarding land use, carbon emissions, biodiversity impact, and water consumption. Ultimately, the successful integration of SCP into aquafeeds could significantly contribute to the industry’s sustainability goals and play an essential role in securing the future supply of raw material proteins.

INVESTIGATION OF THE EFFECTS OF DIFFERENT FEED LINE STRUCTURES ON UWB ANTENNA PERFORMANCE BY CHARACTERISTIC MODE ANALYSIS

This study investigates the effect of various feed topologies on the parameters of a novel ultra-wideband (UWB) antenna using characteristic mode analysis. The proposed design is guided by characteristic mode analysis, commencing with a basic square structure. Initially, a closed-form estimate of the resonance frequencies of the primary modes is provided for the square-shaped structure. Subsequently, the characteristic modes that resonate in the frequency spectrum are modified by introducing symmetrical square slots. To excite these modes and achieve a wideband antenna that encompasses the relevant spectrum, the geometry of the feeding line is altered and its effects are examined. The study incorporates two fundamental feed line geometries: tapered and traditional feed impedance lines. The proposed UWB antennas are fabricated and measured to validate their performance. According to the measurement results a wide impedance bandwidth of 147.82% at -10 dB reference ((0.9–6 GHz)) and stationary radiation patterns across the operating frequency band are obtained for the tapered feed line. The results demonstrate a significant advantage of the tapered feed line over the traditional impedance line. The results for the fabricated prototypes exhibit high similarity to the simulated results. The findings confirm the applicability of the mode analysis method.

Immunomodulatory effect of shrimp by-products in diets for Nile tilapia (Oreochromis niloticus)

Aquaculture plays a critical role in food security, with Nile tilapia (Oreochromis niloticus) being one of the most widely farmed species due to its resilience and adaptability. However, the reliance on traditional feed ingredients poses sustainability challenges. This study aimed to investigate the immunomodulatory effects of incorporating shrimp cephalothorax lipid extract (SCLE) and shrimp cephalothorax meal (SCM) in Nile tilapia diets as sustainable feed alternatives. Two experiments were conducted using diets with varying levels of SCLE (0.4%, 0.8%, 1.2%, and 1.6%) and SCM (5%, 10%, 15%, and 20%). A total of 200 healthy, uniformly sized Nile tilapia fingerlings were selected for two trials with average weights of 4.38±0.13 g and 3.24±0.07 g, respectively. Fish were distributed across 20 tanks and fed ad libitum for 45 days. Hematological parameters were analyzed, revealing that diets with SCLE significantly increased leukocyte, thrombocyte, and hematocrit levels, particularly at 1.6% inclusion. SCM inclusion also led to higher leukocyte counts, especially at 20%. These findings suggest that SCLE and SCM positively impact the innate immune system of Nile tilapia, enhancing their ability to resist pathogens. Additionally, the use of shrimp by-products in aquafeeds presents a viable strategy for reducing environmental waste and promoting more sustainable practices in aquaculture.

Economic Sustainability Foraging Scenarios for Ruminant Meat Production—A Climate Change Adapting Alternative

Climate changes affect all agricultural production systems, directly or indirectly, including that of ruminant meat, through the limitation of forage resources sensitive to reduced water regimes and drought. The present paper assessed the economic sustainability of ruminant meat production in the context of climate change, with a particular focus on integrating bioeconomy principles through the use of drought-resistant crops such as sorghum and millet in livestock feed. This study included scenarios for two farm-level models, a sheep fattening farm and a cattle fattening farm, to determine the economic benefit and impact of integrating resilient crops in the total feed ration. The findings showed that the dry scenario system could offer economic and environmental advantages over traditional water-intensive crops like maize. The results demonstrated that replacing maize with sorghum or millet could result in a reduction in feed costs and enhanced economic benefit over the traditional feed system.

Advancements in synergistic fermentation of probiotics and enzymes for non‐grain feed raw materials

AbstractTo address the escalating challenge of food scarcity and the associated conflicts between human and animal consumption, it is imperative to seek alternative resources that can substitute for traditional feed. Non‐grain feed (NGF) raw materials represent a category of biomass resources that are distinct from grains in their composition. These materials are characterized by their high nutritional content, cost‐effectiveness, ample availability, and consistent supply, which contribute to their significant economic potential. Nonetheless, the extensive application of NGF is currently hindered by several limitations, including a high concentration of antinutritional factors, suboptimal palatability, and an offensive odor, among other shortcomings. The synergistic fermentation of probiotics and enzymes (SFPE) is an innovative approach that integrates the use of a diverse array of enzymes during the feed fermentation process, as well as various strains of probiotics throughout the feed digestion process. This method aims to enhance the nutritional value of the feed, diminish the presence of antinutritional factors, and improve the overall palatability, thereby facilitating the optimal utilization of NGF. This strategy holds the promise of not only replacing conventional feed options but also mitigating the pressing issue of grain scarcity. This paper delves into the practical applications of NGF and presents an overview of the latest research advancements in SFPE fermentation techniques, which can provide cutting‐edge and valuable reference for researchers who devote themselves to research in this field in the future.

Assessing the economic viability and factors affecting farmer adoption of black soldier fly larvae as broiler feed in Bangladesh: a comparative analysis

Despite its impressive economic growth, Bangladesh faces a persistent malnutrition challenge, costing over US$1 billion annually. The broiler industry plays a vital role in addressing nutritional needs by providing essential protein through meat and eggs. However, small and medium enterprises (SMEs) in this sector struggle with high feed prices, impacting their profitability and growth. Black Soldier Fly Larvae (BSFL) offers a sustainable and cost-effective alternative to conventional poultry feed, addressing both rising costs and environmental impacts. This study examined different aspects of BSFL, such as broiler feed, and farmers' perceptions of them in some selected areas of the Sylhet region of Bangladesh. A total of 60 respondents from three subdistricts in Sylhet district were selected using the purposive sampling technique. Data were analyzed through descriptive statistics, discriminative power value, binary logistic regression model, and profitability equation. Among the respondents, 70% (42) had a favorable attitude toward BSFL, and 30% (18) had a highly favorable attitude towards BSFL. Among eight socioeconomic attributes examined for the likeliness of adopting BSFL as broiler feed, the respondents' average income (p < 0.01), years of schooling (p < 0.05), and years of broiler farming experience (p < 0.01) were found to be significant. Further, farms using 50% BSFL and 50% traditional feed had a net return of BDT (Bangladeshi Taka) 35,045.67 (USD 419.71), higher than those using traditional feed, which had a net return of BDT 29670.67 (USD 355.38). Further, farms using 50% BSFL and 50% traditional feed had a benefit–cost ratio (BCR) of 1.16, compared to other farms with a BCR of 1.11. The study also found some problems causing slow growth in the adoption rate of BSFL in the study region, such as lack of training programs, extension campaigns, the uncertainty of input supply, and financial assistance. The study suggests making adequate measures to raise awareness about the profitability of BSFL as broiler feed, conducting training at the root level, and making inputs easily available to farmers.

Insects as Sustainable Feed: Enhancing Animal Nutrition and Reducing Livestock Environmental Impression.

Insects are emerging as a promising alternative source in animal nutrition, offering high protein content and a low environmental impression compared to traditional feed sources. This abstract explores the potential role of insects in reducing the environmental impact of livestock production. Insects such as black soldier fly larvae, mealworms, and crickets are rich in essential nutrients, including proteins, amino acids, fatty acids and so on. They can be efficiently farmed on organic waste streams, contributing to resource efficiency and waste lessening. Additionally, insect farming needs a small quantity of water, less land, and emits fewer greenhouse gases than conventional animal feed production. Despite these benefits, several challenges must be addressed to realize their potential, including regulatory approval, consumer acceptance, production scalability, and ensuring consistent nutritional quality. By overcoming these challenges through research, innovation, and policy support, insects can be integrated into mainstream animal feed systems, significantly reducing the environmental impact of livestock farming and contributing to a more sustainable agricultural future.

Quality and Processability of Meat in Polish Native Pigs – A Review

Abstract The primary aim of pig breeding efforts is to produce animals characterized by outstanding performance, including also fattening and slaughter performance traits. However, improved carcass leanness and reduced carcass fatness, especially intramuscular fat content, have led to a deterioration of pork quality and processability. Due to the growing frequency of meat defects and the limited potential use of such meat in the production of premium products this constitutes a considerable problem for the meat industry. The breeds superior in terms of meat quality are native pig breeds, such as those kept in Poland, i.e. Pulawska (Pul), Zlotnicka Spotted (ZS) and Zlotnicka White (ZW) pigs. The aim of this paper is to present a review of research results concerning quality and processability of meat produced by the Polish native pig breeds. The presented results indicate that pigs of local breeds exhibit a low level of performance traits (daily gain, carcass leanness, backfat thickness) while maintaining very good parameters of meat quality and processability (muscle composition and physical properties, fatty acids profile, sensory traits). Thus, they are used as a source material for premium products. In view of the small size of local populations and higher production costs in Poland it is a niche production. Therefore, the current aim of breeding indigenous breeds is both to maintain a safe population size and genetic diversity and to increase popularity of their meat and processed meat products among consumers. This objective is attained, among other things, thanks to efforts to reduce cost of their production through optimization of environmental conditions and wider use of less expensive traditional feed formulations, which make it possible to fully utilize the potential of local pork resources.

Monitoring the Quality of Vannamei Shrimp (Litopenaeus vannamei) in Traditional Ponds in the Regencies of Lamongan and Gresik, as Suppliers for Fisheries Companies in East Java

Vaname shrimp (Litopenaeus vannamei) is one of Indonesia's leading fisheries commodities. Lamongan and Gresik districts are one of the largest vaname shrimp producers in East Java. This research was conducted to determine differences in the growth of vanami shrimp, differences in the quality of vanami shrimp in terms of color, absolute weight, absolute length, and differences in methods of cultivating vanami shrimp in Rejosari Village, Lamongan Regency and Pandanan Village, Gresik Regency. This research was conducted in April-May 2024 using 4 ponds (2 in Lamongan and 2 in Gresik). The methods used are qualitative and quantitative methods, measuring the color of vanami shrimp using a color reader, testing weight and length using the t-test to determine significant values ​​between the 2 locations, differences in methods of cultivating vanami shrimp using interviews with farmers. The results of this study showed that the color quality of vaname shrimp, A (Rejosari Village, Lamongan) in the 1st replication (pond A1) and (pond A2) doc k2 30-doc to 65 showed quite good color values ​​except for pond A in the 1st replication (pond A1). The results of the absolute weight t-test are 0.30 &gt; 0.05, while for the absolute long t-test it is 0.94 &gt; 0.05. The results of interviews with farmers showed differences in the feed used, farmers A1 and A2 used traditional feed in the form of cassava which was grated and then dried, the artificial feed used for ponds A1 and A2 was (multi super feed). Meanwhile, in ponds B1 and B2, traditional feed was used in the form of steamed leftover rice and corn, the artificial feed used for ponds B1 and B2 was (karka).

Length – Weight Relationship of Rohu (Labeo rohita) Advanced Fingerlings under Sodic Soil Condition

The present study was conducted at the Instructional Fish Farm, College of Fisheries, Acharya Narendra Deva University of Agriculture and Technology, Ayodhya, Uttar Pradesh, in earthen ponds (8m×8m×1m). The experiment for 90 days, using Labeo rohita advanced fingerlings (avg. wt. 30.0 ± 0.5g) under sodic soil conditions. Five treatments were taken in triplicate, with 50 fingerlings per pond. T1 served as the control with traditional feed only (MOC+RB in 1:1 ratio, 5% of total fish body weight). T2 and T3 were fertilized with cattle dung (20 and t ha−1 yr−1) respectively. T4 and T5 received poultry droppings (10 and 7.5 t ha−1 yr−1) respectively. Results showed negative allometric growth (b&lt;3), indicating fish becoming slimmer with length increase. Final weight gain was highest in T4 (110.9±0.9 gm) and T2 (108.7±0.88 gm), and lowest in T1 (70.7±0.49 gm).

Biochemical profile of the native seaweed fly Fucellia maritima (Haliday, 1838) (Diptera: Anthomyiidae) reared on five different substrates

Abstract The sustainability of aquaculture production is crucial for the industry’s long-term viability. Diversification of feed ingredients is a key component in achieving this sustainability. Considered a promising alternative to traditional feed ingredients, terrestrial insect meals often lack sufficient n-3 polyunsaturated fatty acids (PUFA). This study explores the potential of marine insect species, specifically evaluating the influence of five different substrates on the growth and composition of the European native seaweed fly, Fucellia maritima. The substrates tested include a brown seaweed (Fucus sp.), a green seaweed (Ulva sp.), a red seaweed (the invasive Agarophyton vermiculophyllum), the invasive freshwater hyacinth (Eichhornia crassipes), and fish processing waste (codfish Gadus morhua frames). Results show no significant difference in the number of individuals per substrate, although Fucus sp. had a higher total number of individuals completing their life cycle. In contrast, feeding on codfish frames resulted in a lower number of completed life cycles, while E. crassipes led to no survival. Protein content in adult flies ranged from 55.2% to 56.7%, and in pupae, it ranged from 46.1% to 48.7% across different feeding substrates. Notably, pupae fed with Ulva sp., exhibited the highest protein content at 48.7%. Lipid content in adult flies ranged from 10.0% to 13.0%, while in pupae, it ranged from 8.8% to 11.4%. Codfish frames had the highest lipid content in both pupae (11.4%) and adults (13%). The most abundant fatty acid in Fucus sp. – fed pupae and adult flies was oleic acid (18:1 n-9), while palmitoleic acid (16:1 n-7) dominated in other treatments. It is worth highlighting that docosahexaenoic acid (22:6 n-3) was reported for the first time in a seaweed fly, namely when supplied with fish processing waste. These findings suggest that Fucellia maritima may be a promising complementary ingredient for formulating marine aquafeeds.

Transcriptomic profiling reveals potential regulatory genes and molecular mechanisms of residual feed intake in Jian carp (Cyprinus carpio var. Jian)

Feed efficiency is one of the most important economic traits in animal production. Improving feed efficiency through genetic selection or other approaches will reduce production costs and mitigate environmental impacts. Residual feed intake (RFI) has some advantages over traditional feed efficiency metrics as a selection criterion for improving feed efficiency, because it is not a ratio and unrelated to growth. In this study, high RFI (HRFI) and low RFI (LRFI) Jian carp (Cyprinus carpio var. Jian) were tested for feed efficiency traits and serum biochemical indices. No significant differences were observed in the growth rates between HRFI and LRFI group, but daily feed intake of LRFI was significantly lower than that of the HRFI group. The results indicated that significantly higher content of triglyceride (TG) and total cholesterol (T-CHO) in the LRFI group was significantly correlated with RFI. We also selected HRFI and LRFI carp for transcriptomic analysis to determine differentially expressed genes (DEGs) in the liver tissues of 12 individuals. A total of 830 DEGs were identified between HRFI and LRFI group, of which 486 genes were up-regulated and 344 genes were down-regulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed that DEGs were mainly enriched in fatty acid metabolism, ErbB signaling pathway, phosphatidylinositol signaling system, and autophagy. Lipid metabolism and autophagy-related genes, were considered candidate genes that played a crucial role in regulating feed efficiency. Taken together, these results indicated that differences in RFI affect Jian carp metabolism and cell fate, and that alterations in fatty acid metabolism and autophagy are key physiological modes of regulation in low RFI Jian carp. Our data provide valuable candidate genes and candidate pathways, which will help to study the molecular mechanisms of feed efficiency in carp.

The assessment of zeolite addition to diets with or without urea on some productive and physiological indicators in Awassi lambs

BackgroundInterest is growing in the search for alternatives to traditional feed additives, so this study aimed to investigate the effect of adding zeolite to the concentrate diets of Awassi lambs with or without urea on growth performance, nutrient digestion, and health status. A total of 45 Awassi lambs similar in weight (24 ± 2 kg) and age (3 months ± 4 days) were divided into three groups: the first group (G1) fed on a concentrate diet supplemented with 1% urea; the second group (G2) fed on the concentrate diet supplemented with 1% urea and 3% zeolite, and the third group (G3) fed on a urea-free diet supplemented with 3% zeolite.ResultsThe results showed a significant increase (P ≤ 0.05) in final live weight, daily and total weight gain for group G2 compared to groups G1and G3, with a significant superiority (P ≤ 0.05) of group G3 over the control group G1. Significant improvement (P < 0.05) was also observed in feed consumption and feed conversion ratio in diets supplemented with zeolite for groups G2 and G3 compared to the control group G1. Additionally, there was a significant increase (P ≤ 0.05) in nutrient digestion and nutritional value in the diet of group G2 containing urea with added zeolite compared to groups G1 and G3. Zeolite did not affect the levels of glucose, triglycerides, cholesterol, and albumin in the blood, but a significant increase (P ≤ 0.05) in total blood protein level and a significant decrease (P ≤ 0.05) in blood urea level were noted for groups G2 and G3 compared to the control group G1.ConclusionsAdding zeolite to urea-containing concentrate diets improved growth rates and nutrient digestion, of Awassi lambs. In diets without urea, zeolite improved growth rates without affecting nutrient digestion coefficients, in both types of diets, zeolite increased total protein levels and decreased blood urea levels, with all physiological indicators in lambs blood remaining within normal limits.

Relation between complete feeding and blood biochemical indicators in housing of service dogs in a kennel

The purpose of the research was to analyze the relation between the complete feeding and biochemical parameters of blood in service dogs when housing in a kennel in the Tyumen region. The objectives of the research included issues on the assessment and adjustment of the existing diet, the study of the physiological state of dogs and the determination of the economic effi ciency of the proposed diet. The article reveals the problems of non-compliance of the existing diet with the feeding standards for service dogs, especially in terms of vitamin and mineral nutrition. In order to increase the missing indicators such as the content of protein, calcium and phosphorus to the recommended norm, we proposed to include meat and bone meal in the diet in the amount of 15 g, based on 1 kg of live weight of the dog. The ratio of trace elements calcium and phosphorus will be 1.1:1, which corresponds to the norm of 0.8–1.5: 1. The study of the biochemical composition of the blood showed a change in the enzymatic indicators of the serum to the downside, which may be a symptom of vitamin defi ciency in the diet, especially B vitamins (B6, B1). It is necessary to take into account that these indicators increase with physical activity and intensive use of animals. It was found on the based analysis that the cost of a diet with added vitamins and meat and bone meal would be increased by 2 rubles compared to the existing one, but the diet would be balanced in nutrients. When using traditional feed, it is necessary to include balancing additives in the diet, which requires additional costs. At the same time, we can talk about the feasibility of transferring animals from a traditional diet to feeding dry complete feed.

Replacing Cereal with Ultraprocessed Foods in Pig Diets Does Not Adverse Gut Microbiota, L-glutamate Uptake, or Serum Insulin

BackgroundUsing ultraprocessed food (UPF) to replace traditional feed ingredients offers a promising strategy for enhancing food production sustainability. ObjectiveTo analyze the impact of salty and sugary UPF on gut microbiota, amino acids uptake, and serum analytes in growing and finishing pig. MethodsThirty-six Swiss Large White male castrated pigs were assigned to 3 experimental diets: 1) standard (ST), 0% UPF; 2) 30% conventional ingredients replaced by sugary (SU) UPF; and 3) 30% conventional ingredients replaced by salty (SA) UPF. The next-generation sequencing was used to characterize the fecal microbiota. Transepithelial electrical resistance and the active uptake of selected amino acids in pig jejuna were also evaluated. Data were enriched with measurements of fecal volatile fatty acids and serum urea, minerals, and insulin. All data analyses were run in R v4.0.3. The packages phyloseq, vegan, microbiome, and microbiomeutilities were used for microbiota data analysis. The remaining data were analyzed by analysis of variance using linear mixed-effects regression models. ResultsThe UPF did not affect fecal microbiota abundance or biodiversity. The Firmicutes to Bacteroidetes ratio remained unaffected. SU-induced increase in the Anaerostipes genus suggested altered glucose metabolism, whereas SA increased the abundance of CAG-352 and p-2534-18B. No effects on fecal volatile fatty acids were observed. Assumptions of UPF negatively affecting small intestinal physiology were not supported by the measurements of transepithelial electrical resistance in pigs. Active amino acids uptake tests showed potential decrease in L-glutamate absorption in the SA compared with the SU diet. Blood serum analysis indicated no adverse effects on urea, calcium, magnesium, or potassium concentration but the SU group resulted in a lower blood serum insulin concentration at the time of blood collection. ConclusionsWhen incorporated at 30% into a standard growing finishing diet for pigs, UPF does not have detrimental effects on gut microbiota, intestinal integrity, and blood mineral homeostasis.

Alternative ways of using the mushroom substrate as an additive for feed with the purpose of improving animal productivity in agriculture (review)

In modern agriculture, considerable attention is paid to the search for effective and environmentally safe methods of increasing animal productivity. One of the promising directions is the use of mushroom substrate as feed additives. Residual mushroom substrate is often considered as waste, but its processing and use can help reduce feed costs and increase farm profitability. In addition, the use of mushroom substrate helps to reduce the burden on the environment, promoting a closedproduction cycle. In the article it is considered the alternative ways of using mushroom substrate as a feed additive in order to improve the productivity of animals in agriculture. Mushroom substrate, the residual product after growing mushrooms, contains a rich complex of nutrients, such as proteins, vitamins, minerals and other bioactive components. The use of this substrate as a feed additive can provide economic benefits for farms, reducing the cost of traditional feed and increasing animal productivity. Studies indicate a positive effect of the mushroom substrate on the health of animals, in particular on the improvement of immunity and the general physiological state. In addition to economic benefits, the use of mushroom substrate helps to reduce the amount of waste and improve the environmental sustainability of agriculture, meeting the principles of circular economy and sustainable development. Objective. To analyze alternative ways of using mushroom substrate as animal feed additives. In the article it is reviewed modern research and practical experience of using mushroom substrate, its chemical composition and potential benefits for animal husbandry. Special attention is paid to the effect of mushroom substrate on the health of animals, their productivity and general economic indicators of farms. Revealing the potential of mushroom substrate as a feed additive can be an important step in the development of sustainable agriculture, in particular, in the conditions of growing demand for food and resources for its production. Conclusions. The article emphasizes the needfor further research to optimize the methods of using the mushroom substrate in feed rations of various animal species and to develop new technologies for its processing. The practical implementation of these studies can significantly increase the efficiency and competitiveness of agricultural enterprises. Keywords: mushroom substrate, chemical composition, feed additive, animal productivity, agriculture, bioactive components, feeding.

Meat productivity of quail of the Pharaoh breed after drinking a biotechnologicum supplementum (Suspension of Chlorella sorokiniana)

Currently, the use of microalgae as feed additives is gaining more and more popularity in the poultry industry. Microalgae are rich in bioactive metabolites that have demonstrated powerful antioxidant, antiinflammatory, and antiviral properties. Adding a small amount of such an additive to traditional feed has a positive effect on the health and well-being of birds, as well as the quality and quantity of products. The results of the study of meat productivity of quail of the Pharaoh breed after drinking Chlorella sorokiniana suspension are given. The research was conducted on quails during the period of cultivation from 1 to 60 days. To conduct the experiment, two groups (control and experimental) of 30 heads each were formed from day-old birds. Quails were kept in vivarium conditions, in battery cages in compliance with established microclimate requirements. Poultry of both groups received combined feed, developed taking into account age and physiological characteristics. With drinking water, the birds of the experimental group received a chlorella suspension for 60 days. Counting of the quail population and their weighing was carried out weekly, starting from day-old age. The following indicators were determined: preservation, dynamics of live weight, average daily growth of live weight. Based on the results of the control slaughter, the weight of the carcass and the weight of the edible part were determined. Slaughter qualities and morphological composition of quail carcasses were determined by anatomical dissection with determination of the following parameters: pre-slaughter weight, weight of semi-carcasses, carcases of carcases, slaughter yield, weight of edible parts. Drinking chlorella suspension of young quails increased their preservation by 3,83 %, live weight, and absolute growth by 21,0 and 0,57 g (Р&lt;0,05), respectively. Addition of Chlorella sorokiniana suspension to drinking water of quails contributed to an increase in their pre-slaughter weight by 20,0 g, and their weight by 21,0 g compared to the control group. Quail carcasses of the research group were characterized by a higher yield of edible parts compared to birds that consumed clean water. Key words: biotechnology, Chlorella sorokiniana suspension, biotechnological parameters, cultivation, photobioreactor, quails of the Pharaoh breed, body weight, growth, slaughter parameters, weight of carcass, weight of internal organs, output of edible parts.

Enhancing the recovery of complex amino acids from excess sludge via low-intensity ultrasound-assisted enzymatic hydrolysis

The recovery of high-value products such as complex amino acids from activated sludge is a crucial alternative for the recycling of excess sludge, although several challenges still need to be addressed, especially in terms of extraction efficiency and product quality. Herein, a promising approach involving low-intensity ultrasound-assisted enzymatic hydrolysis (UEH) was utilized to obtain complex amino acids from sludge protein. Compared with the values obtained for single enzymatic hydrolysis (EH), after a 20-minute pre-treatment at ambient temperature using low-intensity ultrasound (0.15 W/mL), the enzyme activity increased by 28.7 %, and the degree of protein hydrolysis (DH) correspondingly rose by 93.3 %. The application of ultrasound during subsequent EH resulted in a decrease in DH, with the extent of the decrease increasing over time. This suggested that the primary cause was conformational changes of enzymes rather than mass transfer. Moreover, both the Michaelis–Menten model (R2 > 0.99) and protein hydrolysis model (R2 > 0.96) accurately simulated EH and UEH, indicating that UEH exhibits superior enzyme–substrate affinity and a higher rate of protein hydrolysis. Furthermore, after separation and purification, the total content of seven essential amino acids in the dry material exceeded that in traditional feed sources such as soybean meal. Additionally, the safety test and energy consumption analysis demonstrated that UEH has good potential for the extraction of complex amino acids from excess sludge.

Analysis of the antioxidant activity of toons sinensis extract and their biological effects on broilers.

Plant extracts are rich in a variety of nutrients and contain a large number of bioactive compounds, and compared with traditional feed additives, they have advantages such as wide sources, natural safety and rich nutrition. This study employed in vitro antioxidant and animal experiments to comprehensively evaluate the use of Toona sinensis extract (TSE) in broiler production. 508 1-day-old Cobb 500 broilers were randomly assigned to the 7 experimental groups with 6 replications and 12 birds/replicate. Two groups received Vitamin C (VC) 300 g/t and Vitamin E 500 g/t, and five dose groups of TSE received 0, 300, 600, 900, and 1,200 g/t of TSE in their feed. The study spanned 42 days, with a starter phase (1-21 days) and a finisher phase (22-42 days). The results showed that compared to ascorbic acid, TSE had the scavenging ability of 2,2-Diphenyl-1-picrylhydrazyl and hydroxyl radical, with IC50 values of 0.6658 mg/mL and 33.1298 mg/mL, respectively. Compared to TSE 0 group, broilers fed with 1,200 g/t TSE showed significant weight gain during the starter phase and increased the feed-to-weight gain ratio during both the starter and finisher phases. Additionally, broilers receiving 1,200 g/t TSE had enhanced dry matter and organic matter utilization. Concerning meat quality, broilers in the 1,200 g/t TSE group demonstrated increased cooked meat yield, and pH value, as well as higher antioxidant capacity (T-AOC), dismutase (SOD), and glutathione peroxidase (GSH-PX) in serum. In addition, there was no significant difference in ileal microflora due to TSE supplementation. In summary, this study confirms the positive impact of a dietary inclusion of 1,200 g/t TSE on broiler growth, meat quality, and serum antioxidants.