This study is to investigate the residue level of TCDDs/TCDFs in beef collected from Animal Slaughter Houses in Giwangan (Yogyakarta), Klaten (Central Java) and Kupang (East Nusatenggara). R esidue of persistent organic pollutants (POPs) was also analysed with GC-ECD, and dioxins with GC-MSMS. Fifty samples were collected form Giwangan (20), Klaten (15) and Kupang (15). R esults shows that POPs were detected in beef samples including aldrin, dieldrin, DDT, endrin and heptachlor. The highest total residues of POPs were detected in beef from Giwangan (Yogyakarta) at 93.11 h g/g, followed by Klaten (17.79 h g/g) and Kupang (12.87 h g/g). R esidues of POPs were below the maximum residue limit stated by the SNI 7313: 2008 . A verage of total TEQ for TCDDs/TCDFs in beef from Giwangan was 13,624.38 r g/g with a range between 4,496.66 to 20,642.40 r g / g was higher than that from Kupang at 1,623.98 r g/g with a range between 0.83 to 6,471.07 r g/g. On the other hand, dioxins were not detected in beef from Klaten. The total TEQ of TCDDs/TCDFs in beef samples from Giwangan was 54,497.52 r g/ g and Kupang was 6,495.9 r g/g. The se results indicate d that there wa s an association between the presence of endrin and heptachlor with the presence of TCDDs/TCDFs residues in beef that this is may be new information for further studied.