Total Monounsaturated Fatty Acids Research Articles

Long-term effects of temperature during early life on growth and fatty acid metabolism in age-0 Lake Sturgeon (Acipenser fulvescens)

Environmental temperature during early life may have prolonged effects on growth and fatty acid metabolism, which could strongly influence overwintering survival in the first year of life for temperate-zone fish. In the present study, we examined how temperature during early life history might influence growth performance and fatty acid metabolism in age-0 Lake Sturgeon (Acipenser fulvescens) when exposed to cold temperatures at later stages. Fish were initially at 16 °C and subsequently held at 16 °C or 20 °C for 60 days beginning at 34 days post fertilization (dpf). Then, all fish were subsequently raised at the same temperature of 16 °C until the onset of cold conditioning at 158 dpf where temperature was gradually decreased to 3.5 °C and remained there for two weeks. Samples were collected before (151 dpf) and after cold conditioning (199 dpf) to measure total length, body mass, whole body metabolic rate, fatty acid profile in phospholipids and triglycerides and mRNA expression of genes associated with fatty acid desaturation, elongation and β-oxidation. Results revealed that before cold conditioning, total length and body mass did not differ between temperature groups, but fish raised at 20 °C showed a lower condition factor. During the cold conditioning, only fish raised at 16 °C grew significantly longer and heavier. There was no difference in metabolic rates between treatments. Significant increases in total monounsaturated fatty acids with decreases in total saturated fatty acids were identified in phospholipids and triglycerides in both temperature groups after the cold conditioning; however, the 20 °C group did not significantly increase levels of gene expression associated with fatty acid desaturation (SCD and FADS1) whereas the 16 °C group did. Our results suggest that thermal experience during early life may influence overwintering survival of age-0 Lake Sturgeon.

Serum Levels of Free Fatty Acids in Obese Mice and Their Associations with Routine Lipid Profiles.

PurposeTo investigate serum levels of free fatty acids (FFAs) and their associations with routine serum lipids in diet-induced obese mice, which have been scantily reported before.MethodsMale C57BL/6 J mice were fed high-fat diets for 12 weeks to induce obesity. Levels of serum FFAs were measured by ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry.ResultsObese mice had higher serum levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C), but lower triglycerides (TG) than control mice. A total of 30 FFAs were found, and 3 saturated fatty acids (SFAs), all 8 monounsaturated fatty acids (MUFAs) and 7 polyunsaturated fatty acids (PUFAs) decreased in obese mice, but one SFA (C4:0) increased. Differences in the relative levels of individual FFAs to total FFAs, SFAs, MUFAs or PUFAs between obese and control mice were different from each other and from those evaluated by concrete levels except C4:0, C16:1, C19:1 and C18:4. Only the concrete levels of C4:0, C22:3 and C18:4 were associated with routine serum lipids, including C22:3 negatively with TG in control mice, and C4:0 and C18:4 positively with LDL-C in obese mice, although the relative levels of C4:0 to total MUFAs negatively with TC, and C23:3 to total SFAs or MUFAs negatively with TG in control mice. Different relative levels of the remaining FFAs were differently associated with different routine serum lipids in obese and/or control mice.ConclusionObesity may influence serum FFAs profiles. The relationship of individual FFAs and their relative levels to other FFAs with routine serum lipids in obese and control mice suggests that individual FFAs may interact with others and obesity on levels of routine serum lipids. Once confirmed, the interactions may be novel perspectives when fatty acids are used to improve hyperlipidemia in the subjects with obesity.

Effect of hempseed meal on performance, egg quality and egg yolk fatty acids content in laying quails

In this study, the laying performance, external egg quality, egg yolk colour and fatty acids profile of quails (Coturnix coturnix japonica) fed on diets containing hemp seed meal (HSM) were determined. During 8 weeks trial, a total of 150, 10-weeks-old laying quails were used. Five diets were formulated to contain HSM at the level of 0 (control),5, 10, 15, and 20% that represented as 0 HSM, 5 HSM, 10 HSM, 15 HSM and 20 HSM, respectively.The performance parameters were not significantly (P>0.05)influenced by the dietary HSM contents. Eggshell ratio, eggshell breaking strength, egg shape index, egg yolk index and egg yolk colour values were not significantly (P>0.05) influenced by the dietary HSM, whereas eggshell thickness was significantly (P<0.05) affected. The albumen index has been significantly (P<0.05) increased by increasing in the HSM level in the diets. The HSM supplementation to the diets was effective on fatty acid composition and total saturated fatty acids, total mono unsaturated fatty acids and total polyunsaturated fatty acids content of the egg yolk depending on the addition level. In conclusion, HSM can be used to increase egg total monounsaturated fatty acids and especially omega-3 fatty acids without unfavourable effects on the performance and egg quality parameters.

Dietary Malic Acid Supplementation Induces Skeletal Muscle Fiber-Type Transition of Weaned Piglets and Further Improves Meat Quality of Finishing Pigs.

The aim of this study was to investigate effects of dietary malic acid supplementation on skeletal muscle fiber-type transition during nursery period and the subsequent meat quality of finishing pigs. Results showed that malic acid supplementation for 28 days increased oxidative fiber percentage of weaned piglets, accompanied by the increased aerobic oxidation in serum and longissimus thoracis (LT) muscle. Additionally, activities of total antioxidant capacity and glutathione peroxidase in serum were increased. Moreover, dietary malic acid supplementation during nursery period tended to increase pH24h and significantly decreased drip loss in LT muscle of finishing pigs. The content of total saturated fatty acid (SFA) and total monounsaturated fatty acid in LT muscle was significantly decreased, whereas the ratio of polyunsaturated fatty acid to SFA tended to increase. Together, dietary malic acid supplementation during nursery period can effectively increase antioxidant capacity and oxidative fibers percentage of weaned piglets, and further improve water holding capacity and nutritional values of pork in finishing pigs.

Dietary Fat and Polyunsaturated Fatty Acid Intakes during Childhood Are Prospectively Associated with Puberty Timing Independent of Dietary Protein.

Dietary fat and fat quality have been inconsistently associated with puberty timing. The aim of this study was to investigate the prospective associations of dietary fat, saturated fatty acid (SFA), polyunsaturated fatty acid (PUFA), and monounsaturated fatty acid (MUFA) with puberty timing. Using longitudinal data from China Health and Nutrition Survey (CHNS) and Southwest China Childhood Nutrition and Growth (SCCNG) Study, we analyzed dietary data, anthropometric measurements, and potential confounders. Dietary intakes were assessed by 3-day 24-h recalls. Age at Tanner stage 2 for breast/genital development (B2/G2) and age at menarche/voice break (M/VB) were used as puberty development markers. Cox proportional hazard regression models were used to estimate the relevance of dietary intake of total fat, SFA, PUFA, and MUFA on puberty timing. Among 3425 girls and 2495 boys, children with higher intakes of total fat and PUFA were more likely to reach their B2/G2 or M/VB at an earlier age. Associations were not attenuated on additional adjustment for childhood dietary protein intake. However, higher intakes of SFA or MUFA were not independently associated with puberty development. A higher intake of dietary fat and PUFA in prepuberty was associated with earlier puberty timing, which was independent of dietary protein intake.

Effects of dietary insect (Ephestia kuehniella) egg meal on growth performance and fattyacid profile of common carp (Cyprinus carpio)

The effects of mill moth (Ephestia kuehniella) egg meal (IEM) upon growth performance and fatty acid profile of common carp (Cyprinus carpio) (1.12 ± 0.01 g) were evaluated in triplicate groups for 12 weeks. Three isonitrogenous (35% CP) and isocaloric (17 MJ kg?1) diets were formulated replacing fishmeal with 0% (G-0), 50% (G-50), and 100% (G-100) IEM. The results of the trial indicated that G-50 had the highest growth performance values and a more efficient FCR (1.79). Replacement levels of IEM did not have any significant effect on survival. Total monounsaturated fatty acids (MUFA) were found to be statistically different for all diets, and Σ SFA in fish was decreased with the increasing IEM levels. The ratio of n3/n6 was higher in G-0 reflecting a better balance. The results suggested that 50% replacement of IEM with fishmeal enhanced growth performance in common carp without any adverse effect on fatty acid profile, but investigation of the long-term effects has still been needed.

The inclusion of detoxified castor bean meal in sugarcane silage increases the carcass weight and the content of unsaturated fatty acids in lamb meat.

The aim of this study was to evaluate the performance and meat quality of lambs fed sugarcane silage containing increasing levels of detoxified castor bean meal (DCBM). Twenty-four Santa Inês male sheep were used, with initial body weight of 25.34 ± 2.57 kg, distributed in a completely randomized design with four treatments (0, 70, 140, and 210 g/kg of DCBM in the sugarcane silage). Intake and digestibility of dry matter (DM) and crude protein (CP) were positively influenced (P < 0.05) by the levels of DCBM. There was a linear increase (P < 0.05) on the intake and digestibility of neutral detergent fiber with the inclusion of DCBM. The daily and total gains showed a quadratic effect (P < 0.05) with the inclusion of DCBM, with a maximum point of 241.64 g/d and 18.12 kg at the level of 161.9g/kg of DCBM. There was a linear increase (P < 0.01) on the hot carcass with the inclusion of DCBM. The inclusion of DCBM did not influence (P > 0.05) the levels of total lipids (2.1%) in lamb meat, but the levels of oleic acid (C18:1 n-6c) and total monounsaturated fatty acids presented a quadratic effect (P < 0.05). It is recommended to include 160 g/kg of detoxified castor bean meal in sugarcane silage.

Effects of low salinity on fatty acid and free amino acid composition of muscle tissues in Portunus trituberculatus

The effects of low salinity on fatty acid and free amino acid (FAA) composition in muscles of swimming crab, Portunus trituberculatus, were investigated in this study. The swimming crabs were reared for 5 weeks under two different experimental treatments (the 12 ppt group and the 24 ppt group). Then, composition of fatty acids and free amino acids in total muscles was measured using gas chromatography–mass spectrometry (GC-MS) with external standard method and liquid chromatography–mass spectrometer (LC-MS) with internal standard method respectively. Results of fatty acid analysis showed that C18:1n9c, C20:5n3 (EPA) and C22:6n3 (DHA) were the dominating fatty acids in muscle tissues of P. trituberculatus. There was an extremely significant increase in contents of total fatty acids (TFA), total saturated fatty acids (SFA), total monounsaturated fatty acid (MUFA) and total polyunsaturated fatty acid (PUFA) in muscle tissues of P. trituberculatus with low salinity treatment (12 ppt) compared with those cultured in seawater (24 ppt) (p < 0.01). Results of free amino acid analysis showed that the content of total free amino acids (TFAA) significantly decreased in muscle tissues of P. trituberculatus with low salinity treatment compared with those in seawater (p < 0.05). Among all flavour-tasting FAAs, the umami FAA content was extremely significantly higher in low salinity group compared with control group (p < 0.01); the sweetish FAA content was significantly lower in low salinity group while the bitter taste of FAA content had no significant differences between two groups.

Low Dietary n6/n3 Ratio Attenuates Changes in the NRF 2 Gene Expression, Lipid Peroxidation, and Inflammatory Markers Induced by Fructose Overconsumption in the Rat Abdominal Adipose Tissue.

The objective of this study was to examine the benefits of different n6/n3 polyunsaturated fatty acid ratios on the lipid metabolism, insulin resistance, and oxidative stress in the adipose tissue of rats fed a high-fructose diet. Male and female rats were divided into four groups: a control group (CON) (n6/n3 ratio ~7), a high-fructose group (HF) (n6/n3 ratio ~7), an N6-HF group (n6/n3 ratio ~50), and the DHA-HF group (n6/n3 ratio ~1, with the addition of docosahexaenoic (DHA) and eicosapentaenoic (EPA) acid). The CON group received plain water and the HF group received 15% fructose in their drinking water. Fructose induced an increase in the content of serum triglycerides, serum cholesterol, and HOMA-IR index. Among the fatty acids, elevated proportions of C18:1n9 and C16:1n7, as well as an increase in total monounsaturated fatty acid (MUFA), were found in the adipose tissue of the HF group. Fructose treatment also changed oxidative parameters, including a marked increase in the serum malondialdehyde (MDA) content. Meanwhile, DHA supplementation caused a significant decrease in the serum MDA concentration in comparison with the HF group. In addition, DHA/EPA supplementation attenuated oxidative stress by increasing NRF 2 gene expression. Fructose treatment also significantly decreased the adiponectin level, while DHA supplementation ameliorated it. The changes observed in this trial, including the decrease in the content of DHA and EPA, the decreased EPA/ARA ratio, and the increase in the expression of inflammatory genes, are characteristics of the low-grade inflammation caused by fructose treatment. These changes in the rat adipose tissue could be prevented by dietary intervention consisting of DHA supplementation and a low n6/n3 ratio.

Effects of dietary betaine supplementation on growth performance, meat quality, muscle fatty acid composition and antioxidant ability in slow-growing broiler chickens

ABSTRACT 1. This study investigated the effects of dietary betaine supplementation on growth performance, meat quality, muscle fatty acid composition and antioxidant ability in slow-growing broiler chickens. 2. In total, 400, one-day-old female Xueshan broiler chicks were randomly divided into five groups with eight replicates of ten chickens each for 102 d. Broilers were fed a basal diet supplemented with 0, 125, 250, 500 or 1,000 mg/kg betaine. 3. Broilers fed betaine had better feed conversion efficiency and weight gain (P < 0.05) and increased meat redness and yellowness 24 h after slaughter. Supplementation linearly decreased cooking loss and drip loss from breast muscle (P < 0.05). Muscular resilience was improved and tenderness increased (P < 0.05). Intra-muscular saturated fatty acids decreased, while total monounsaturated fatty acids and polyunsaturated fatty acids increased (P < 0.05). Betaine increased activities of glutathione peroxidase (GPx) and total superoxide dismutase (SOD), glutathione (GSH) level, ratio of reduced glutathione/oxidised glutathione, and activity of scavenging hydroxyl radicals. It increased the activity of total antioxidant capacity (T-AOC) in the breast muscle (P < 0.05). Moreover, supplementation up-regulated (P < 0.05) mRNA expression levels of blood and antioxidant markers. 4. In conclusion, 1000 mg/kg betaine can be recommended as a supplement for slow-growing, Xueshan chicken.

Sex-Specific Differences in the Associations Between Omega-6 Polyunsaturated Fatty Acids and Type 2 Diabetes in Chinese People.

Background: Some evidence indicates a potential beneficial effect of omega-6 polyunsaturated fatty acids (n-6 PUFAs) on type 2 diabetes mellitus (T2DM); however, the findings to date remains inconclusive and little is known about whether sex modifies these associations. Therefore, this study aimed to investigate potential sex-specific differences in this associations among Chinese adults.Methods: We conducted a cross-sectional study in an area of Dalian city, China; Chinese men and women who attended the Department of Clinical Nutrition and Metabolism between January and December 2020 were invited to participate in this study. All participants were assessed for basic demographic characteristics, fasting blood glucose, HbA1c, and other serum biomarkers and serum phospholipid FAs.Results: In total, 575 Chinese adult participants (270 men and 305 women) were included in the analysis. Hypertension and dyslipidaemia were more common among men than women, but there were no significant differences between the sexes in fatty acid composition, except for eicosadienoic acid (EA; 20:2n-6) and total monounsaturated fatty acids (MUFA). The age-adjusted OR for having T2DM in the highest quartile of arachidonic acid (20:4n-6) level was 0.47 (95% CI, 0.22, 0.98) in men, and this association remained consistently significant in the fully adjusted multivariate models. In contrast, no significant associations between n-6 PUFAs and T2DM risk were observed in women, regardless of model adjustment.Conclusions: In conclusion, these results demonstrate a notable sex-specific differences in the associations between n-6 PUFAs and T2DM. Higher n-6 PUFA status may be protective against the risk of T2DM in men.

The Quality Characteristic and Fatty Acid Profile of Cold-Pressed Hazelnut Oils during Nine Months of Storage

Poland is one of the largest producers of hazelnuts in Europe; however, information regarding the storage of cold-pressed hazelnut oil is limited. Thus, the aim of this study was to determine the oxidative indices and fatty acid composition of oils from six hazelnut cultivars during 9 months of storage. At the beginning of storage, the hazelnut oils showed zero or very low oxidation values, which indicated the absence of initial triglyceride hydrolysis and fatty acid oxidation. Acid values increased with storage time, which was statistically significant, ranging from 0.17 to 0.34 mg KOH/g oil. The peroxide value in the first 5 months of storage was undetectable, whereas after 9 months the oils showed a slight increase in oils obtained from the ‘Olbrzym z Halle’ cultivar, followed by the ‘Barcelonski’ cultivar, at 3.39 and 2.15 meq O2/kg, respectively. The lipid content of the kernels was very stable under storage conditions. Total monounsaturated fatty acid content exhibited the highest proportion, while saturated fatty acids (SFAs) had the lowest content over the entire storage period. The percentage of polyunsaturated fatty acids showed a small decrease during storage, but was not statistically significant; therefore, polyunsaturated fatty acid remained stable. The percentage of monounsaturated fatty acids decreased by approx. 1.6%, thus the percentage of SFA increased by approx. 13.7% during 9 months of storage. The oil yield ranged from 69% for nuts from the ‘Nottinghsamski’ cultivar to 75% from the ‘Webba Cenny’ and ‘Barcelonski’ cultivars.

Intake, performance, meat quality and fatty acid profile of Nellore bulls finished in feedlot with diets containing dry corn gluten feed

We evaluate the effects of dry corn gluten feed (DCGF) levels in the finishing diet of Nellore cattle on feed intake, performance, carcass traits, meat quality and fatty acid profile. A hundred twenty non-castrated Nellore bulls (Bos indicus), with an average initial body weight of 361.66 ± 14.85 kg were used. Animals received feedlot diets containing different levels of DCGF (0, 18, 36 and 54%, dietary DM basis). This corn by-product, which is rich in fiber and protein, improves intake, performance, and hot carcass weight of animals, without impairing meat color and shear force (SF). Although backfat thickness was similar between the control and the DCGF treatments, there was a tendency towards a reduction in the intramuscular fat (IMF) content of Longissimus thoracis (LT) muscle, and a change of the IMF fatty acid profile of LT muscle at levels above 18% DCGF. Greater amounts of conjugated linoleic acid (CLA), saturated fatty acid (SFA), and monounsaturated fatty acids (MUFA) were found in animals finished with diets containing 18 and 36% DCGF when compared to the control diet, which may reflect the changes in rumen pH and biohydrogenation in response to changes in dietary carbohydrate source. Moreover, these results could be due the greater amounts of total SFA and MUFA found in DCGF compared to ground corn and soybean meal.

In vivo performances, ileal digestibility, and physicochemical characterization of raw and boiled eggs as affected by Tenebrio molitor larvae meal at low inclusion rate in laying quail (Coturnix japonica) diet

A total of 120, twelve wk old female Japanese quails were divided into 4 groups (6 replicates of 5 birds each). The control group (CON) fed a corn-soybean diet; in the other 3 groups, Tenebrio molitor larvae meal (TML) replaced 5, 10, and 20% of the soybean protein (T5, T10, and T20). The laying performance and egg quality were studied for 54 d. The data were processed by a one-way ANOVA; the orthogonal contrast analysis was performed to test the linear, quadratic and cubic effects among the means. The laying rate and egg mass linearly decreased (P < 0.01) as the TML inclusion level in the diet increased. The egg weight and feed conversion ratio linearly increased from the control to T20 diet (P < 0.01) while the digestibility of dry matter, organic matter, and crude protein linearly decreased (P < 0.05). The albumen and yolk weight showed a linear increase (P < 0.01) due to dietary TML inclusion, while the eggshell weight showed the opposite (P < 0.05). The estimated activity of Δ9-desaturase (C16:0), Δ5+Δ6-desaturase on both polyunsaturated fatty acid n-6 and n-3 linearly increased (P < 0.05) as affected by dietary TML. The boiled yolk lightness (L*) showed higher values in T5 and T10 groups (quadratic contrast, P < 0.01). The yolk redness index (a*) showed lower values in T5 and T20 than control and T10 groups (cubic contrast, P < 0.01). The albumen L*, a*, and b* indexes showed a significant effect of the quadratic contrast (P < 0.05). In addition, the albumen b* index showed a significant effect of the cubic contrast (P < 0.01). The total lipids showed the highest values (cubic contrast, P < 0.05) in the T10 and T20 groups. The total monounsaturated fatty acids linearly increased (P < 0.05) according to the increase of dietary TML. The best inclusion level of defatted TML meal for laying quails seems to be 1.4% of diet, corresponding to the T5 diet.

Nutritional Enhancement of Health Beneficial Omega-3 Long-Chain Polyunsaturated Fatty Acids in the Muscle, Liver, Kidney, and Heart of Tattykeel Australian White MARGRA Lambs Fed Pellets Fortified with Omega-3 Oil in a Feedlot System.

Simple SummaryThe problem addressed in this research was the possibility of enhancing the nutritional value and health beneficial omega-3 long-chain fatty acid content of lamb and its edible components. The aims and objectives were to evaluate the omega-3 contents of muscle, liver, kidney, and heart of lot-fed Tattykeel Australian White lambs of the MARGRA brand, in response to dietary supplementation with or without omega-3 oil fortified pellets. The findings demonstrate that the inclusion of omega-3 oil in feedlot diets of lambs enhances the human health beneficial omega-3 long-chain polyunsaturated fatty acid profiles of edible muscle tissue and organs without compromising meat quality or shelf life. These results are valuable to society because of increased functionality, health benefits, micro-marbling, tender, mouth-melting taste, and high-end eating quality experience of MARGRA lamb tissues and organs.The aim of this research was to evaluate the nutritional enhancement of omega-3 long-chain polyunsaturated fatty acid (n-3 LC-PUFA) composition of edible lamb Longissimus thoracis et lumborum muscle, heart, kidney, and liver in response to dietary supplementation of lot-fed lambs with or without omega-3 oil fortified pellets. The hypothesis tested was that fortifying feedlot pellets with omega-3 oil will enhance the human health beneficial n-3 LC-PUFA composition of edible lamb muscle tissue and organs. Seventy-five Tattykeel Australian White lambs exclusive to the MARGRA brand, with an average body weight of 30 kg at six months of age, were randomly assigned to the following three dietary treatments of 25 lambs each, and lot-fed as a cohort for 47 days in a completely randomized experimental design: (1) Control grain pellets without oil plus hay; (2) Omega-3 oil fortified grain pellets plus hay; and (3) Commercial whole grain pellets plus hay. All lambs had ad libitum access to the basal hay diet and water. Post-slaughter fatty acid composition of the Longissimus thoracis et lumborum muscle, liver, kidney, and heart were determined using thee gas chromatography–mass spectrophotometry technique. Results indicated significant variations (p < 0.05) in fatty acid profiles between tissues and organs. Omega-3 oil fortified pellets significantly (p < 0.05) increased ≥C20 n-3 LC-PUFA (C20:5n-3 eicosapentaenoate, EPA + C22:5n3 docosapentaenoate, DPA + C22:6n3 docosahexanoate DHA); C18:3n-3 alpha-linolenate, ALA; C18:2 conjugated linoleic acid, CLA; total monounsaturated fatty acids, MUFA; polyunsaturated fatty acids, PUFA contents; and reduced the ratio of omega-6 to omega-3 fatty acids in all lamb organs and tissues without impacting shelf-life. The findings demonstrate that the inclusion of omega-3 oil in feedlot diets of lambs enhances the human health beneficial omega-3 long-chain polyunsaturated fatty acid profiles of edible muscle tissue and organs without compromising meat quality.

Gökkuşağı Alabalık (Oncorhynchus mykiss Walbaum, 1792) Kıyması ile Üretilmiş Geleneksel Türk İçli Köftesinin Besinsel Komposizyonu ve Dondurularak Depolama Boyunca (-18ºC) Renk ve Duyusal Kalitesinin Belirlenmesi

The nutritional value of stuffed meatballs made with minced rainbow trout rather than red meat in the stuffing was investigated, as well as color variations (L *, a *, and b *) and sensory quality, using two different cooking methods (boiling and frying) on samples stored in the freezer (-18 oC). As a result of this research, the moisture, the crude ash, the lipid, and the crude protein contents of stuffed meatballs produced with rainbow trout were found as %58.25 ± 0.93, %2.01 ± 0.05, %5.28 ± 0.29, and %7.69± 0.08, respectively. The content of total saturated fatty acids composition (∑SFA), total monounsaturated fatty acids composition (∑MUFA), and total polyunsaturated fatty acids composition (∑PUFA) was found to be 12.52 ± 0.23%, 39.11 ± 1.27%, and 43.73 ± 1.98%, respectively. The atherogenic (AI) and thrombogenic indexes (TI) for dietary factors associated with cardiovascular disease were found to be 0.10 and 0.25, respectively. In the evaluation of color (L *, a *, and b *) of frozen samples, L * (black-white) and b * (blue-yellow) values in all groups (raw, boiled, and fried) increased significantly at the end of storage compared to the initial value ( p&amp;lt;0.05). Following frying and boiling, the * (green-red) values of the frozen-stored samples decreased significantly (p&amp;lt;0,05). Sensory quality was assessed in this study using two cooking methods: boiling and fried, both of which are common serving methods for stuffed meatballs. Although a significant decrease was detected in all sensory quality parameters of samples during frozen storage, it was determined that it did not reach the unacceptable limit at the end of storage.

Effect of Different Slaughter Weights on Meat Quality, Fatty Acids and Flavor Component of Korean Woori Black Pig Loin and Belly

The present study was undertaken to investigate the quality characteristics of Korean Woori black pig (KWP) bellies and loins by different slaughter weight (SW) groups. The loin and belly samples collected from KWPs with different body weights (50, 75, 90, 105, and 120 kg) at 24 h post-mortem were used in the present investigation. The samples were analyzed for quality traits, fatty acid profiles, and volatile flavor compounds. Results showed that the fat content of the loin (8.64%) and belly samples (46.78%) was significantly higher in the 120 kg SW group compared to those of other SW groups (p<0.05). However, a lower protein content (12.20-12.67%) was found in the belly cuts of the heavier SW groups (105-120 kg) compared to those of the lighter SW groups (p<0.05). The lowest cooking loss (24.34%) was found in the loin cuts of the 120 kg SW group (p<0.05). Both the loin and belly cuts were observed to be redder in color with increasing SW (p<0.05). Higher oleic acid (C18:1, n9) and total monounsaturated fatty acid content and lower linolenic acid(C18:3, n3) and total polyunsaturated fatty acid content were observed in both cuts of the 120 kg SW group (p<0.05). Out of the flavor compounds identified, 11 and 17 compounds in the loin and belly, respectively, were associated with the SW. An increase in the SW resulted in increased concentrations of C18:1n9- and amino acid-derived flavor compounds. Overall, the meat samples of the heavier SW groups (120 kg) exhibited better quality and higher concentrations of volatile compounds associated with pleasant flavors. However, the meat of the 120 kg SW group also contained a much higher fat level (8.64 and 46.78% in the loin and belly, respectively) that may result in high trimming loss and hence a high rejection risk by consumers.

Fatty Acid Composition of Cannabis sativa, Linum usitatissimum and Camelina sativa Seeds Harvested in Lithuania for Food Use.

The objective of this study was to determine species-associated differences in the seed proximate and fatty acid composition of three traditional oil crop species, hemp (Cannabis sativa), flax (Linum usitatissimum), and camelina (Camelina sativa), and the sowing season of camelina harvested under Lithuanian farming conditions for food use. Camelina seeds had the highest (p < 0.001) content of protein, oil, and sugar contents compared to both dehulled hemp and flax seeds. The amounts of protein and oil in camelina seeds were considerably increased by their summer cultivar, which showed higher (p < 0.001) contents of protein and oil than winter cultivars. However, the highest and lowest (p < 0.001) fiber content was found in flax seeds and camelina seeds, respectively. Camelina seeds showed considerably higher and lower (p < 0.001) proportions of total monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids, respectively, compared with hemp and flax. The summer cultivar of camelina had higher (p < 0.001) proportions of saturated (SFA) and MUFA and lower proportions of PUFA compared with winter cultivars. Hemp seeds had the highest and lowest (p < 0.001) proportions of PUFA and MUFA, respectively. The n-6/n-3 PUFA ratio in hemp seeds is optimal (3.79), whereas the use of flax and camelina seeds with their n-6/n-3 ratios of 0.28 and 0.48, respectively, can significantly improve this ratio in the overall diet. The properties of oil crop seeds showed that whole seeds of hemp, flax, and camelina are potentially highly beneficial to human health.

Chemometric Analysis of Fatty Acid Composition of Raw Chicken, Beef, and Pork Meat with Plant Extract Addition during Refrigerated Storage.

During the shelf-life, meat undergoes a number of processes that negatively affect the quality of the product, including fatty acid composition. The application of various plant extracts in meat could affect the changes of fatty acids during storage. Thus, the aim of this study was to investigate the effect of various spice and herb extracts on fatty acid composition in raw pork, beef, and chicken meat when stored at 4 °C for 13 days. Based on multivariate statistical analysis, two datasets were extracted from each type of meat. One dataset included samples with allspice, bay leaf, black seed, cardamom, caraway, clove, and nutmeg with the high share of total MUFA (monounsaturated fatty acids) in chicken and pork meat and high MUFA and PUFA (polyunsaturated fatty acids) contribution in beef meat after storage. The second dataset included basil, garlic, onion, oregano, rosemary, and thyme with high PUFA share in chicken and pork meat and high SFA (saturated fatty acids) contribution in beef meat. From the regression analysis, a significant effect of time on fatty acid composition in meat was reported. Generally, the rates of fatty acid changes were dependent on the plant extract incorporated into the meat. The most visible effect of plant extracts was obtained in chicken meat. In chicken meat with plant extracts, the rates of SFA and PUFA changes with time were slower compared to the control sample. In summary, the fatty acid composition of intramuscular fat varied during storage, and the addition of plant extracts significantly affected the rate of these changes, which was dependent on the meat matrix.

Effects of Dietary Protein and Lipid Levels on Growth, Body Composition, Blood Biochemistry, Antioxidant Capacity and Ammonia Excretion of European Grayling (Thymallus thymallus)

This study evaluated growth, body composition, antioxidant capacity, innate immunity and ammonia excretion of European grayling (Thymallus thymallus) fed diets containing different protein and lipid contents. Six diets were produced to contain 30, 40, or 50% protein and 10 or 20% lipid. Juvenile fish averaging 25.2 ± 0.28 g were stocked into eighteen 450-L circular tanks in a recirculating aquaculture system (RAS) and fed the test diets to satiation twice daily for 12 weeks. Fish weight gain (WG) was enhanced (P &amp;lt; 0.05) as dietary protein increased from 30% (229% WG) to 40% (262% WG) and plateaued thereafter. Enhancing protein and lipid content of diet led to reduced feed intake. Also, feed efficiency was improved by increasing dietary protein (by 40.8%) and lipid (by 16.5%) levels. An interaction of protein and lipid was found on whole-body lipid, and muscle lipid content increased as dietary lipid level increased. Muscle arachidonic acid (ARA), eicosapentaenoic acid (EPA, 20:5n-3) and total n-6 long-chain polyunsaturated fatty acids (LC-PUFA) contents enhanced by increasing dietary protein level. Moreover, increasing fat content of diet led to enhanced muscle linoleic acid, linolenic acid, total monounsaturated fatty acids (MUFA), total n-6, ratio of docosahexaenoic acid (DHA, 22:6n-3) to EPA and n-6/n-3. However, EPA, DHA, total n-6 LC-PUFA, total n-3, total n-3 LC-PUFA, and EPA/ARA ratio decreased at higher dietary lipid level. Serum triglyceride (TG) level and lactate dehydrogenase (LDH) activity decreased as dietary protein level increased. Increasing fat content of diet led to enhanced serum TG, cholesterol and glucose concentrations and reduced alanine aminotransferase, aspartate amino transferase and LDH activities. Serum malondialdehyde concentration was enhanced by increasing both dietary protein and lipid. Furthermore, serum myeloperoxidase activity was enhanced at higher dietary lipid level. Water ammonium nitrogen (NH4+-N) concentration was measured after 5 and 24 h of last feeding, and the results indicated the reduction of ammonia excretion as dietary lipid content increased. These findings suggest that 40% dietary protein can support optimal growth of juvenile European grayling reared in RAS and increasing lipid content from 10 to 20% can improve feed utilization and reduce ammonia excretion to the rearing water.