Total Mn Research Articles

Molecular simulations of hydrated phyllomanganates

Hydrated phyllomanganates are layered Mn-oxide minerals with interlayers that can possess variable water contents and react strongly with trace metals due to octahedral vacancies in the layer. The unique properties of phyllomanganates afford them a significant role in many environmental phenomena that affect soil and water composition mainly via cation exchange and adsorption of trace metals. Slight variations in the structural and chemical composition often result in a dramatic difference in the chemical reactivity of the minerals. Molecular simulations at the classical mechanical level of theory, which uses a simplified description of the interatomic potential energies, can provide an atomistic perspective of the relationship between the chemical composition and the bulk and interlayer structures. We introduce a set of interatomic potentials for hydrated phyllomanganates with variable vacancy and Mn3+ content and report the classical mechanical simulation results performed at standard temperature and pressure. The potentials we introduce provide not only a reasonable reproduction of the experimentally determined atomic structures of the chalcophanite group, but also new insights on similar phyllomanganate minerals with hexagonal symmetry and a range of vacancy contents. When a vacancy is protonated, Mn3+ is unstable in the hexagonal birnessite layer and occupies the interlayer as a cap on the associated vacancy. When a vacancy was charge-balanced by Mn3+ and Na+, considerable amounts of Mn3+ were incorporated into the hexagonal birnessite layer, but only at total Mn(III) contents greater than ∼10% and with disordered layer stacking. The potentials also predicted a vacancy-free, triclinic Na-birnessite structure with Mn(III)-rich rows in the layer which were arranged parallel to the b-axis and separated by two rows of Mn(IV) octahedra. The dominant interlayer Na complex at a water content ≥0.7 H2O/MnO2 was an octahedrally-coordinated, split interlayer site with two birnessite O atoms in the axial positions and four interlayer H2O in the equatorial positions. Other interlayer Na complexes including some edge- and face-sharing complexes existed in trace amounts (<10%). These classical mechanical simulations represent a successful first test of the introduced interatomic potentials, which can be used to further explore the interlayer and surface complexes of phyllomanganate and birnessite-group minerals.

Zn, Ni, Mn, Cr, Pb and Cu in soil-tea ecosystem: The concentrations, spatial relationship and potential control

Excessive accumulation of heavy metals in tea has certain health risk to the consumers. To discuss the spatial relationship between heavy metals in tea leaves and soil in a county scale, 74 samples of tea leaves and corresponding subsoil (15–30 cm) were collected in the tea plantations of Lishui, Nanjing, China. The total Zn, Ni, Mn, Cr, Pb and Cu in both tea leaves and soil samples, as well as pH value in soil were analyzed. The results of regression showed that the Mn and Cr in tea leaves were increased when the Mn and Cr in soil were at high levels, and higher pH decreased Zn, Ni, Mn and Cu contents in tea leaves. Moreover, distance to village and metal industry had negative impacts on the Mn level in soil. It is necessary to control the pH of soil in tea plantations which are close to villages or metal industries, as well as, to search more appropriate areas, where are far away from villages or metal industries, to plant tea should be taken into consideration. In addition, 17.57% of the samples have higher concentration of Pb than the maximum limits was found in tea plantation of Lishui. Heavy metals (Cr) standards regarding leafy vegetables is used for reference in this study, as there are no maximum levels of contaminants (MLC) in tea. It is significant to construct a targeted standard of heavy metals contents that could applicable throughout the world for reduce the health risks to the consumers.

Distribution trends and ecological risks of arsenic and trace metals in wetland sediments around gold mining activities in central-southern and southeastern Côte d'Ivoire

The distribution patterns and sources of arsenic and trace metals (Cd, Pb, Cu, Zn, Cr, Co, Fe, Al, Mn, and Ni) were investigated in wetland sediments that may be impacted by industrial and artisanal gold mining, along with those from non-mining areas in Divo (Bonikro and Agbaou sites) and Aboisso (Afema site) Departments, central-southern and south-eastern Côte d'Ivoire, respectively. Ecological risks of sediments were examined using the potential ecological risk index (PERI) and sediment quality guidelines (SQGs). Sediments from mining activities showed significantly higher total As, Pb, Cr, Ni, Mn, Cu, and Al concentrations than the ones from non-mining areas at Afema and Agbaou. On the contrary, a reverse spatial trend was observed for Al, Cr, and As at Bonikro. Geoaccumulation index showed moderate to strong contamination in Cd whatever the sites and activity types, and a relatively low contamination in Pb, Cu, Zn, Cr, Co, Fe, Al, Ni, and Mn, confirmed by the enrichment factor. Extreme sediment contamination in As was observed in industrial mining areas at Afema. Artisanal mining was the main source of Mn and Ni, especially at Agbaou. On the contrary, Cd, Al, Pb, Co, Cu and Zn and Cr were mainly from lithogenic sources. Principal component and hierarchical cluster analyses showed that total organic carbon controls distributions of elements Ni and Cu at Afema, Cd and Cr at Agbaou, and Mn and Co at Bonikro. Based on PERI and SQGs, As could pose high risks to biota in industrial mining areas.

Boron-zinc interaction in the absorption of micronutrients by cotton

B-Zn interaction modifies the nutritional dynamics of copper (Cu), iron (Fe) and manganese (Mn) in cotton. The main objective of this research was to evaluate the effect of B and Zn concentrations on the absorption of Cu, Fe and Mn in cotton plants grown in a nutrient solution. A completely randomized experimental design with three replicates was performed, in a 4×5 factorial scheme, corresponding to four concentrations of B (0, 20, 40 and 80 μM L-1) and five concentrations of Zn (0, 1, 2, 4 and 8 μM L-1). At 115 days after emergence, the plants were collected, divided into roots, shoots and fruits, and chemically analyzed. The results allowed to conclude that the Cu content and total Cu in the fruit, total Cu in the roots, Cu efficiency, Fe content in the roots, Fe absorption efficiency, Mn content in the fruit, and Mn absorption efficiency of cotton are influenced by the concentrations of B in the solution. The interaction between B and Zn affected the total Fe in the roots, Fe content and total Fe content in the fruit, Fe transport efficiency, total Mn in the shoots and Mn transport efficiency. In addition, Zn acts differently according to the supply of B and vice versa.

Arsenic and Antimony in Hydrothermal Plumes from the Eastern Manus Basin, Papua New Guinea

Studies on the concentrations of arsenic (As) and antimony (Sb) in seawater columns are very important for tracing hydrothermal plumes and understanding fluid characteristics of seafloor hydrothermal systems. The total As, Sb, Mn, and Cl− concentrations of three hydrothermal plume seawater column samples have been studied at Stations 18G, 18K, and 18B in the eastern Manus back-arc basin, Bismarck Sea, Papua New Guinea. At Stations 18G and 18K, the plumes above North Su and near the Suzette site in the SuSu Knolls hydrothermal field are both enriched in As, Sb, and Mn and depleted in Cl, as a result of contribution of As-Sb-Mn-enriched and Cl-depleted vent fluid outputs to the hydrothermal plume, which is most likely generated in the subseafloor by fluid-rock interaction, magma degassing, or phase separation (boiling of hydrothermal fluid). The plume at Station 18B is enriched in As, Sb, Mn, and Cl, suggesting that As-Sb-Mn-Cl-enriched fluid discharges from vents, which have been generated by fluid-rock interaction. The concentrations of As and Sb anomalous layers, like manganese (Mn), are higher than those of the other layers in the three hydrothermal plume seawater columns. As and Sb with Mn showed a positive correlation (R2&gt;0.8, p&lt;0.05), and the distributions of As and Sb within the hydrothermal plume are not controlled by particle adsorption or biogeochemical cycles, suggesting that As and Sb, like Mn, can be used to detect and describe the characteristics of hydrothermal plumes in seawater environment. In addition, anomalous layer with As/Sb ratio lower than those of ambient seawater at the same temperature is found in the eastern Manus basin, suggesting that the As/Sb ratio may also act as an effective tracer reflecting the effect of hydrothermal activity on As and Sb in the seawater column.

Analysis of Groundwater Quality Surrounding Municipal Solid Waste Landfill: Banyuurip Landfill, Magelang, Indonesia

Banyuurip landfill generates leachate containing suspended and dissolved matter, including some heavy metals, a result of waste degradation and decomposition of organic materials. Leachate can infiltrate the soil then contaminate groundwater. Once the leachate infiltration reaches the soil and groundwater, it will contaminate the surrounding environment. In this study, the contamination of leachate into ground water was investigated by measuring the concentration of Manganese (Mn), Iron (Fe) and Free Ammonia (NH3-N) in leachate and dug wells surrounding the landfill. The concentration of Mn, Fe and NH3-N in leachate was found to be 5.42 mg/L, 6.16 mg/L, and 1652 mg/L, respectively. The concentration in the surrounding dug wells located within 500 m from the landfill varied from 0.00 – 0.86 mg/L for free ammonia, 0.00 – 0.31 mg/L for total Fe, and 0.00 – 0.26 mg/L for total Mn. The concentration of Mn, Fe and NH3-N decrease with increasing distance between the landfill and wells.

Quantifying Changes to the Electrolyte and Negative Electrode in Aged NMC532/Graphite Lithium-Ion Cells

A set of LiNi0.5Mn0.3Co0.2O2/graphite lithium-ion cells underwent 750 charge-discharge cycles during about 8 months at 55°C to upper cutoff potentials of 4.0, 4.1, 4.2, 4.3, and 4.4 V. The electrolyte in these cells was extracted using a centrifuge method and studied using gas chromatography/mass spectrometry to determine the changes to the solvents and by inductively coupled plasma-mass spectrometry to determine the changes to the salt content in the electrolyte. The negative electrodes from the cells were harvested and studied by micro-X-ray fluorescence to quantify the amount of transition metals which migrated from the positive electrode to the negative electrode during the testing. Emphasis is given to a detailed description of the quantitative methods used in the hope that others will adopt them in similar studies of different types of aged lithium-ion cells. The cells studied here initially had 1.1 molal LiPF6 ethylene carbonate (EC): ethyl methyl carbonate (EMC) (3:7 by weight) electrolyte. The aged cells showed increasing amounts of dimethyl carbonate and diethyl carbonate created by transesterification as the upper cutoff potential increased. Only extremely small amounts of Mn, less than 0.1% of the total Mn in the positive electrode, were found on the negative electrode after this aggressive testing.

A procedure for the measurement of Oxygen Consumption Rates (OCRs) in red wines and some observations about the influence of wine initial chemical composition

The rates at which wine consumes oxygen are important technological parameters for whose measurement there are not accepted procedures. In this work, volumes of 8 wines are contacted with controlled volumes of air in air-tight tubes containing oxygen-sensors and are further agitated at 25 °C until O2 consumption is complete. Three exposure levels of O2 were used: low (10 mg/L) and medium or high (18 or 32 mg/L plus the required amount to oxidize all wine SO2). In each oxygen level, 2–4 independent segments following pseudo-first order kinetics were identified, plus an initial segment at which wine consumed O2 very fast. Overall, multivariate data techniques identify six different Oxygen-Consumption-Rates (OCRs) as required to completely define wine O2 consumption. Except the last one, all could be modeled from the wine initial chemical composition. Total acetaldehyde, Mn, Cu/Fe, blue and red pigments and gallic acid seem to be essential to determine these OCRs.

Macro, trace and toxic elements of 4 different edible wild plants from Karadeniz Region

The study was conducted to assess the content (mg kg -1 fresh wt.) of macro, trace and toxic elements in the 4 different edible wild plants. The percentage of dry matter and ash ranged from 6.77 to 20.56 and 0.79 to 2.26, respectively. The contents of Ca (1074), Fe (160.5), Mn (18.21), Ni (6.11), B (2.61), Cr (0.93), Co (0.50), Mo (1.80), Be (0.009) and Se (0.076) of U. diocia were richer than in other plants. Other hand, K (7742), Mg (954), Al (10.79), V (9.77) and Ag (0.109) in T. orientalis , Zn (12.47), Cu (9.98) and Ba (130.3) in O. umbellatum were taken the highest value. Also As, Hg, Tl, Cd and Pb were determined mg kg -1 level in fresh plants. Conversely, antimony (Sb) in wild plants could not be detected by ICP-MS. The results of statistical analysis of forty plants showed that moisture, total dry matter, ash, K, Ca, Mg, Fe, Zn, Cu, Mn, Ba, Ni, Al, V, B, Cr, Co, Mo, Be, Se, Ag, As, Tl, Cd and Pb contents were varied significantly compared to plant species except for Na and Hg (p<0.05). Consequently, wild plants may be used as popular vegetables in many people diet as a source of minerals (Fe, Cu, Mn, Cr, Mo, K, Zn and Mg). Excessive plant consumption may be adversely affected human health with Cd, As, Hg, Tl and Pb.

Intracellular Distribution of Manganese by the Trans-Golgi Network Transporter NRAMP2 Is Critical for Photosynthesis and Cellular Redox Homeostasis.

Plants require trace levels of manganese (Mn) for survival, as it is an essential cofactor in oxygen metabolism, especially O2 production via photosynthesis and the disposal of superoxide radicals. These processes occur in specialized organelles, requiring membrane-bound intracellular transporters to partition Mn between cell compartments. We identified an Arabidopsis thaliana member of the NRAMP family of divalent metal transporters, NRAMP2, which functions in the intracellular distribution of Mn. Two knockdown alleles of NRAMP2 showed decreased activity of photosystem II and increased oxidative stress under Mn-deficient conditions, yet total Mn content remained unchanged. At the subcellular level, these phenotypes were associated with a loss of Mn content in vacuoles and chloroplasts. NRAMP2 was able to rescue the mitochondrial yeast mutant mtm1∆ In plants, NRAMP2 is a resident protein of the trans-Golgi network. NRAMP2 may act indirectly on downstream organelles by building up a cytosolic pool that is used to feed target compartments. Moreover, not only does the nramp2 mutant accumulate superoxide ions, but NRAMP2 can functionally replace cytosolic superoxide dismutase in yeast, indicating that the pool of Mn displaced by NRAMP2 is required for the detoxification of reactive oxygen species.

Liver mitochondrial oxygen consumption and efficiency of milk production in lactating Holstein cows supplemented with copper, manganese and zinc.

The objective of this study was to examine the relationship between mitochondrial proton leak and feed efficiency with supplementation of different levels of Cu, Mn and Zn (Bioplex, Alltech) at levels above Nutrient Requirements of Dairy Cattle (NRC, 2001). Milk yield and composition, mineral excretion in milk and faeces, feed efficiency and liver mitochondrial proton leak were measured in 60 Holstein dairy cows at approximately 70days in milk on a commercial dairy. Treatments reflect total Cu, Mn and Zn intake/day and are as follows: (i) High: 444mg/day Cu, 3492mg/day Mn, 2234mg/day Zn; (ii) Med: 436mg/day Cu, 3002mg/day Mn, 2047mg/day Zn; (iii) Low: 420mg/day Cu, 2764mg/day Mn, 2186mg/day Zn; (iv) LowMn: 391mg/day Cu, 2617mg/day Mn, 1849mg/day Zn; and (v) Control: 264mg/day Cu, 2850mg/day Mn, 1593mg/day Zn. Proton leak-dependent respiration was lowest in Control (p<.10). However, measures of efficiency were greatest in Med and least in High (p<.10). Therefore, measures of efficiency did not reflect efficiency due to low proton leak and there appears to be an upper limit to beneficial supplementation of Cu, Mn and Zn.

Comparison between snowmelt-runoff and rainfall-runoff nonpoint source pollution in a typical urban catchment in Beijing, China.

As a climate-driven event, nonpoint source (NPS) pollution is caused by rainfall- or snowmelt-runoff processes; however, few studies have compared the characteristics and mechanisms of these two kinds of NPS processes. In this study, three factors relating to urban NPS, including surface dust, snowmelt, and rainfall-runoff processes, were analyzed comprehensively by both field sampling and laboratory experiments. The seasonal variation and leaching characteristics of pollutants in surface dust were explored, and the runoff quality of snowmelt NPS and rainfall NPS were compared. The results indicated that dusts are the main sources of urban NPS and more pollutants are deposited in dust samples during winter and spring. However, pollutants in surface dust showed a low leaching ratio, which indicated most NPS pollutants would be carried as particulate forms. Compared to surface layer, underlying snow contained higher chemical oxygen demand, total suspended solids (TSS), Cu, Fe, Mn, and Pb concentrations, while the event mean concentration of most pollutants in snowmelt tended to be higher in roads. Moreover, the TSS and heavy metal content of snowmelt NPS was always higher than those of rainfall NPS, which indicated the importance of controlling snowmelt pollution for effective water quality management.

Vertical Distribution Characteristics of Fe and Mn in Subtropical Reservoirs During Summer

Iron (Fe) and manganese (Mn) are redox-sensitive elements that are both important chemical indicators of water quality. To study their vertical distribution characteristics in south-subtropical reservoirs during summer, Fe and Mn concentrations in the water column, as well as the physical and chemical conditions of the water, were investigated in the lacustrine zones of nine reservoirs in the eastern of Guangdong Province, in July 2016. These results showed that Fe and Mn exhibited a strong concentration gradient in deep reservoirs due to thermal stratification. The total Fe (TFe), total Mn (TMn), dissolved Fe (DFe), and dissolved Mn (DMn) concentrations in the surface water are significantly lower than in these in bottom zones (TFe, F=6.708, P=0.032; TMn, F=9.720, P=0.014; DFe, F=8.129, P=0.029; DMn, F=11.125, P=0.016). The average concentrations of TFe and TMn are 0.399 mg·L-1 and 0.422 mg·L-1, respectively at the bottom five of the deep reservoirs, and the dissolved ions accounted for more than 70% of this concentration. In shallow reservoirs without thermal stratification, TFe, TMn, DFe and DMn concentrations in surface water are lower than those measured at bottom of the reservoir, no significant difference was detected (TFe, F=0.135, P=0.726; TMn, F=0, P=1; DFe, F=0.006, P=0.943; DMn, F=0.007, P=0.936). The average concentrations of TFe and TMn are 0.110 mg·L-1 and 0.089 mg·L-1, respectively at the bottom of the four shallow reservoirs, and they were present predominantly in particulate form. The concentrations of TFe and DFe correlated significantly (P<0.05) with the dissolved oxygen (DO) concentration, pH and depth, but did not significantly correlate with TN or TP concentration in the deep reservoirs. The gradient distributions of DO and pH levels is critical to the gradient distribution of Fe and Mn in deep reservoirs because of water stratification. Low DO concentrations and acidic conditions in these areas contributed to the release of Fe and Mn from sediment and result in high dissociation of these elements in the bottom of deep reservoirs. Simultaneously, high concentrations of Fe and Mn at the bottom of reservoirs may also attribute to the characteristic red soil of this region. The results from this study imply that the high concentrations of Fe and Mn common in the bottom of deep reservoirs is associated with the stable stratification of these reservoirs in the summer. The concentrations of Fe and Mn may be exceed water standards when deep water is taken for public water provision. The problem may be avoided with surface water supplying.

Altered Expression of a Malate-Permeable Anion Channel, OsALMT4, Disrupts Mineral Nutrition.

Aluminum-activated malate transporters (ALMTs) form a family of anion channels in plants, but little is known about most of its members. This study examined the function of OsALMT4 from rice (Oryza sativa). We show that OsALMT4 is expressed in roots and shoots and that the OsALMT4 protein localizes to the plasma membrane. Transgenic rice lines overexpressing (OX) OsALMT4 released malate from the roots constitutively and had 2-fold higher malate concentrations in the xylem sap than nulls, indicating greater concentrations of malate in the apoplast. OX lines developed brown necrotic spots on the leaves that did not appear on nulls. These symptoms were not associated with altered concentrations of any mineral element in the leaves, although the OX lines had higher concentrations of Mn and B in their grain compared with nulls. While total leaf Mn concentrations were not different between the OX and null lines, Mn concentrations in the apoplast were greater in the OX plants. The OX lines also displayed increased expression of Mn transporters and were more sensitive to Mn toxicity than null plants. We showed that the growth of wild-type rice was unaffected by 100 µm Mn in hydroponics but, when combined with 1 mm malate, this concentration inhibited growth. We conclude that increasing OsALMT4 expression affected malate efflux and compartmentation within the tissues, which increased Mn concentrations in the apoplast of leaves and induced the toxicity symptoms. This study reveals new links between malate transport and mineral nutrition.

Long-term irrigation with zinc smelter effluent affects important soil properties and heavy metal content in food crops and soil in Rajasthan, India

ABSTRACTUse of wastewater for irrigating agricultural crops is on the rise, particularly in the developing countries. The present study was undertaken to assess the long-term effect of irrigation with zinc smelter effluent on important soil properties including heavy metal status. Metal concentration in the edible parts of the crops grown on smelter effluent-irrigated soils was also measured. For this purpose, the agricultural lands which have been receiving the zinc smelter effluent irrigation for about five decades at Debari, Udaipur, India were selected. The adjacent tubewell water-irrigated fields were selected as reference. Long-term irrigation with smelter effluent resulted into significant buildup of ethylenediaminetetraacetic acid extractable Zn (57.7 fold), Cu (4.51 fold), Fe (3.35 fold), Mn (1.77 fold), Ni (1.20 fold), Pb (45.1 fold), and Cd (79.2 fold) in soils over tubewell water-irrigated fields. Total Zn, Cu, Mn, Ni, Pb, and Cd content in effluent-irrigated soils was also increased by 27.0, 1.60, 1.40, 1.30, 26.2, and 167 fold, respectively. Risk assessment indicated a very high to moderate potential ecological risk due to Cd, Pb, and Zn in soils close to the immediate vicinity of the smelter plant. Cadmium and Pb concentrations in edible parts of almost all the crops grown on effluent-irrigated soils were above the safe limit of CODEX commission. On an average, soil pH dropped by 0.31 units due to smelter effluent irrigation. Smelter effluent irrigation resulted into significant increase in soil organic carbon, electrical conductivity, and CaCO3 content. On an average, there was decrease in available N (21.0%) and P (20.8%) content in effluent-irrigated soils over the tubewell water-irrigated ones. An increase in available K (102%) and S (26.0 fold) was recorded in effluent-irrigated soils. Long-term irrigation with zinc smelter effluent resulted into reduced microbial activities in soil as evidenced from the level of microbial biomass carbon and dehydrogenase activity. In view of the buildup of heavy metals and subsequent imbalance in essential plant nutrients in smelter effluent-irrigated soils, appropriate remediation-cum-fertilization strategy needs to be adopted for better soil health and plant nutrition.

Application of Circular Bubble Plume Diffusers to Restore Water Quality in a Sub-Deep Reservoir.

Circular bubble plume diffusers have been confirmed as an effective technology for the restoration of the deep water system, but have never been applied in sub-deep water system. In this study, circular bubble plume diffusers were used, for the first time, to restore water quality in the Aha Reservoir, a typical sub-deep reservoir in Southwest China. Axisymmetric intrusive gravity currents were formed with a horizontal radius of 250 m at the equilibrium depth and the intrusion of oxygen-enriched water occurred within the depth of 10–14 m, while thermal stratification remained intact. A total of 95% of the imported oxygen was dissolved, but most was consumed by organic matter and other reduced substances within the hypolimnion. The oxygen consumption of organic matter, NH4+ and remaining reduced materials, accounted for 41.4–52.5%, 25% and 13.3–24.4% of the total imported oxygen, respectively. Compared with the control sites, dissolved oxygen level in the hypolimnion increased 3–4 times, and concentrations of NH4+, total Fe and total Mn were reduced by 15.5%, 45.5% and 48.9%, respectively. A significant decrease in total phosphorus and nitrogen concentrations was observed in the experimental zone (0.04–0.02 mg/L and 1.9–1.7 mg/L, respectively). This indicates that circular bubble plumes have great potential for oxygenation of the hypolimnion and improving water quality in the sub-deep water system. Nevertheless, further efforts are needed to improve the discrete bubble model to elaborate the oxygen transmission dynamics and the plume formation processes in sub-deep water systems, incorporating oxygen consumption processes.

Outdoor grazing of dairy cows on pasture versus indoor feeding on total mixed ration: Effects on gross composition and mineral content of milk during lactation

The influence of feeding system and lactation period on the gross composition, macroelements (Ca, P, Mg, and Na), and trace elements (Zn, Fe, Cu, Mo, Mn, Se, and Co) of bovine milk was investigated. The feeding systems included outdoor grazing on perennial ryegrass pasture (GRO), outdoor grazing on perennial ryegrass and white clover pasture (GRC), and indoors offered total mixed ration (TMR). Sixty spring-calving Holstein Friesian dairy cows were assigned to 3 herds, each consisting of 20 cows, and balanced with respect to parity, calving date, and pre-experimental milk yield and milk solids yield. The herds were allocated to 1 of the 3 feeding systems from February to November. Milk samples were collected on 10 occasions over the period June 17 to November 26, at 2 or 3 weekly intervals, when cows were on average 119 to 281 d in lactation (DIL). The total lactation period was arbitrarily sub-divided into 2 lactation periods based on DIL, namely mid lactation, June 17 to September 9 when cows were 119 to 203 DIL; and late lactation, September 22 to November 26 when cows were 216 to 281 DIL. With the exception of Mg, Na, Fe, Mo, and Co, all other variables were affected by feeding system. The GRO milk had the highest mean concentrations of total solids, total protein, casein, Ca, and P. The TMR milk had the highest concentrations of lactose, Cu, and Se, and lowest level of total protein. The GRC milk had levels of lactose, Zn, and Cu similar to those of GRO milk, and concentrations of TS, Ca, and P similar to those of TMR milk. Lactation period affected all variables, apart from the concentrations of Fe, Cu, Mn, and Se. On average, the proportion (%) of total Ca, P, Zn, Mn, or Se that sedimented with the casein on high-speed ultracentrifugation at 100,000 × g was ≥60%, whereas that of Na, Mg, or Mo was ≤45% total. The results demonstrate how the gross composition and elemental composition of milk can be affected by different feeding systems.

Erratum to: Forms of Cu (II), Zn (II), and Pb (II) compounds in technogenically transformed soils adjacent to the Karabashmed copper smelter

The aim was to study Cu (II), Zn (II), and Pb (II) forms in technogenically transformed soils adjacent to the Karabashmed copper smelter. Studies were performed in the plume zone of the Karabash smelter and in the floodplains of Ryzhii Brook and Sak-Egla River. Geomorphological and geochemical migration processes prevail in technogenic landscapes. The differentiation of landscape-geochemical conditions plays the dominant role, which determines the localization of metals. The total Mn, Cr, Ni, Cu, Zn, Pb, Cd, and As contents and the macroelement compositions of soils were determined by X-ray fluorescence. The composition of Cu, Pb, and Zn compounds in soils was determined by the Tessier sequential fractionation. The determination of the geochemical fractions of heavy metals in soils is a key issue in the study of their mobility. The metals were fractionated into the following five fractions: exchangeable, bound to carbonates, bound to Fe and Mn oxides, bound to organic matter, and residual fractions. It is shown that the total Zn and As contents in the 0- to 5-cm layer of soils on monitoring plots exceed their lithosphere clarks in hundreds of times, and the total Cu, Pb, and Cr contents exceed their lithosphere clarks in tens of times. Factors and processes controlling the distribution and transport of Cu, Pb, and Zn forms in soils were determined. According to landscape-geochemical differentiation, the eluvial (automorphic) catena (plot T4) takes the main technogenic load of dust fallouts from the Karabash copper smelter. The accumulation of material brought from above and the geochemical precipitation of discharges from tailings dumps occur in superaqual catenas (plots T1, T2, and T3). In the technogenically transformed soils, the basic stabilizers of the mobility of Cu is organic matter, for Pb it is Fe-Mn (hydro) oxides, and for Zn - it is clay minerals. The distributions of Cu, Zn, and Pb forms in the studied technogenically transformed soils are due to a number of factors: First, these are the composition of technogenic pollutants contaminating ecosystems and the time during which the contamination occurred, and second, this is the combination of physicochemical properties controlling the buffer properties of the polydisperse system of soils and parent materials.

Effect of Lime, Compost and Microbial Inoculants on Micronutrient Removal by Mustard and Maize in Trace Metal Contaminated Soil of Jharkhand

A field experiment was conducted on trace metal contaminated soil at Patratu (Ramgarh) to study the effect of lime, compost, plant growth promoting rhizobacteria and arbuscular mycorrhizal fungi on micronutrient removal viz. Zn, Cu, Mn and Fe in mustard-maize cropping system. Results reveal that inoculation with Glomus mossae, Pseudomonas striata and Azotobacter chroococcum increased Zn concentration to the extent 13 to 32, 10 to 24 and 9 to 24 (%), respectively over control. Copper, manganese and iron uptake followed almost similar trend as that of Zn. Microbial inoculants with or without vermicompost increased the trace metal removal, however, vermicompost alone decreased the removal. It was observed that microbial inoculations reduced the total Zn, Cu, Mn and Fe content in soil. However, available micronutrients were significantly reduced by microbial inoculation and amendments.

Influence of harvest managements of festulolium and tall fescue on biomass nutrient concentrations and export from a nutrient-rich peatland

This study was designed to show the effects of harvest time and frequency on biomass nutrient concentrations (total ash, N, P, K, Ca, Mg, Fe, Mn, Cu and Zn) as well as total nutrient removal potential by festulolium and tall fescue cultivated on a nutrient-rich fen peatland. The harvest managements included a three-cut (3C) and three two-cut (2C) systems which differed by two-week delays of first cut as: 2C-early, 2C-mid and 2C-late, representing phenological stages of pre-heading, inflorescence emergence, and flowering, respectively. In the study year, the grasses received 80-16–60kg N-P-K ha−1 in spring and 80-0–100kg N-P-K ha−1 after each harvest (except final). Nutrient concentrations and total ash content in the biomass dropped sharply during spring-summer growth. Concentrations of Ca, Mg and Mn were significantly higher in tall fescue biomass during spring-summer growth, but for other nutrients, there were no consistent differences between the two grasses. Total ash in the biomass from 3C managements (1.1Mg ha−1) was ∼28% higher than in the biomass removed by 2C managements (0.8 Mg ha−1). Similarly, mean annual N removal by 3C (315kgNha−1) was 31% higher than by 2C (240kgNha−1) managements, but net removals (removed minus applied N) from both managements were similar (75–80kgha−1). Net P-removal by 3C (37kgP ha−1) was higher than by 2C (26kg P ha−1) managements, whereas total K removals in all managements were close to the applied K amount (i.e., net removals were close to zero). Whereas all crop/management combinations extracted more N and P than applied, the 2C-late approach is recommended since the biomass yield was higher than for the other 2C approaches, and it required less fertilizer and management inputs than the 3C approach.