Total Magnesium Research Articles

Effect of plastic mulch residue on plant growth performance and soil properties

Plastic mulch is widely utilized for weed control, temperature regulation, soil erosion prevention, disease management, and soil structure improvement, ultimately enhancing crop quality and yield. However, a significant issue with conventional plastic mulches is their low recycling rates, which can cause plastic residue to build up, thereby damaging soil quality and reducing crop yield. The emergence of biodegradable films offers a promising solution to mitigate this issue and reduce soil pollution. However, its potential effects on soil properties and plant performance remain unclear. In this study, low-density polyethylene (LDPE) and poly (butylene succinate-co-butylene adipate) (PBSA) were used to observe the effect of plastic mulch residues on soil properties and plant growth performance via potting experiment. Additionally, the interaction effects of compost and biochar as soil amendments with plastic mulch residues were also evaluated. The result of this study revealed that the type of plastic significantly affected the total nitrogen and magnesium uptake; however, the morphological traits of the tested plant (Japanese mustard spinach) were not significantly affected. The addition of compost and biochar led to a significant increase in both shoot and total dry weight of the plant, indicating a positive effect on its growth. The results of the two-way ANOVA indicated a significant influence of plastic type on dissolved phosphate (PO43−) levels and soil dehydrogenase activity (DHA). The interaction effect (plastic type with soil amendment) was statistically significant only for soil DHA. Neither plastic mulch residues nor soil amendments significantly affected other soil chemical properties. However, long-term experiments to systematically investigate the long-term effects of plastic residues are necessary.

The association between dietary approaches to stop hypertension diet and bone mineral density in US adults: evidence from the National Health and Nutrition Examination Survey (2011–2018)

This study aimed to investigate the relationship between the dietary approaches to stop hypertension (DASH) dietary patterns and bone mineral density (BMD) in adults residing in the United States. To achieve this, data from the National Health and Nutrition Examination Survey (NHANES) database for 2011–2018 were utilized. This study utilized the NHANES database from 2011 to 2018, with a sample size of 8,486 US adults, to investigate the relationship between the DASH diet and BMD. The DASH diet was assessed based on nine target nutrients: total fat, saturated fat, protein, fiber, cholesterol, calcium, magnesium, sodium and potassium. The primary outcome measures were BMD values at the total BMD, thoracic spine, lumbar spine, and pelvis. Multivariable linear models were employed to analyze the association between the DASH diet and BMD. Interaction tests, subgroup, and sensitivity analysis were also followed. A negative correlation was observed between the DASH diet and total BMD (OR: − 0.003 [95%CI: − 0.005, − 0.001), pelvic (OR: − 0.005 [95%CI: − 0.007, − 0.002]), and thoracic BMD (OR: − 0.003 [95%CI: − 0.005, − 0.001]). However, the DASH diet does not appear to have a particular effect on lumbar spine BMD (OR: − 0.002 [95%CI: − 0.004, 0.001]). Similarly, when the DASH diet was categorized into tertiles groups, the relationship with total BMD, pelvic BMD, thoracic BMD, and lumbar spine BMD remained consistent. Furthermore, we performed a sensitivity analysis by converting BMD to Z-scores, and the results remained unchanged. Subgroup analyses and interaction tests indicated no significant dependence of BMI, gender, smoking, hypertension, and diabetes on the observed association (all p for interactions > 0.05). The DASH diet has been identified as potentially reducing total BMD, while specifically impacting thoracic and pelvic BMD. However, it appears to have no significant effect on lumbar spine BMD.

How much magnesium sulfate is needed to "keep total serum magnesium above 2.0 mg/dL"?

For patients at increased risk of life-threating ventricular arrythmias, hospitalists often administer intravenous magnesium sulfate to maintain total serum magnesium concentration (TsMg) above 2 mg/dL. How long each dose keeps TsMg above this threshold is not well known, however. We collected TsMg values from 12,618 veterans who were given 24,363 doses of intravenous magnesium sulfate during 14,901 hospitalizations for acute heart failure. Across dose amounts, the average TsMg dropped below 2.0 mg/dL within 24 h of administration. When we limited our analysis to 2 g doses (the most common dose) and adjusted for baseline TsMg, estimated glomerular filtration rate, oral magnesium supplementation, and loop diuretic dosing, we found that less than half of the adjusted TsMg values remained above 2.0 mg/dL just 12 h after dose administration. Hospitalists should expect, on average, to administer 2 g intravenous magnesium sulfate at least twice daily to maintain total serum magnesium above 2 mg/dL.

The effect of nutritional biochemical indexes on the hospitalization outcome of COVID-19.

Aims to investigate the relationship between nutritional biochemical indexes and hospitalization outcomes of COVID-19 patients, 132 continuous patients with COVID-19 from December 2022 to January 2023 in Lishui hospital were retrospectively analyzed, and the nutritional biochemical indexes in peripheral blood, such as total protein, albumin, calcium, phosphorus, and magnesium, were detected. Meanwhile, the levels of several cytokines and PBMC subtypes (CD4, CD3, CD8, NK and B cells) were detected too. The Spearman correlation analysis, one-way ANOVA and multivariate logit regression were conducted. Results suggested that the levels of total protein and albumin were significantly decreased in patients with poor outcomes, and the levels of calcium, phosphorus, and magnesium were significantly correlated with hospitalization outcomes. COVID-19 patients with diabetes had higher levels of IL-6 and IFN-γ than those patients without diabetes. The levels of IL-2, IFN-γ, IL-6 and Il-10 in the dead patients were significantly higher than those in the recovery and worse patients. Total protein and albumin were significantly positively correlated with levels of NK and B, CD4, CD8, CD3 lymphocytes. The levels of CD4, CD8 and CD3 lymphocytes were significantly decreased in dead patients than other patients. Multivariate logit regression analysis suggests that lymphocyte number, albumin and IL-6 are independent risk factors to evaluate the hospitalization outcome. In summary, nutritional biochemical indexes were significantly corelated with cytokines and PBMC subsets, and had an impact on the severity of COVID-19 patients. Improvement of low protein malnutrition is broad-spectrum and basic strategy to improve the hospitalization outcome of COVID-19.

Response of leaf functional traits to soil nutrients in the wet and dry seasons in a subtropical forest on an island.

Island ecosystems often have a disproportionate number of endemic species and unique and fragile functional characteristics. However, few examples of this type of ecosystem have been reported. We conducted a comprehensive field study on Neilingding Island, southern China. The leaf samples of 79 subtropical forest tree species were obtained and their functional traits were studied in the dry and wet seasons to explain the relationships between plant functional traits and soil nutrients. We found a greater availability of soil moisture content (SMC) and nutrients in the wet season than in the dry season. The values of wet season soil available phosphorus (5.97 mg·kg-1), SMC (17.67%), and soil available potassium (SAK, 266.96 mg·kg-1) were significantly higher than those of the dry season. The leaf dry matter content, specific leaf weight, leaf density, leaf total carbon, leaf total nitrogen, leaf total calcium, and the N/P and C/P ratios of leaves were all significantly higher in the dry season than in the wet season, being 18.06%, 12.90%, 12.00%, 0.17%, 3.41%, 9.02%, 26.80%, and 24.14% higher, respectively. In contrast, the leaf area (51.01 cm2), specific leaf area (152.76 cm2·g-1), leaf water content (0.59%), leaf total nitrogen (1.31%), leaf total phosphorus (0.14%), and leaf total magnesium (0.33%) were much lower in the dry season than in the wet one. There were significant pairwise correlations between leaf functional traits, but the number and strength of correlations were significantly different in the dry and wet seasons. The SAK, soil total phosphorus (STP), and pH impacted plant leaf functional traits in the dry season, whereas in the wet season, they were affected by SAK, STP, pH, and NO3- (nitrate). Both soil nutrients and water availability varied seasonally and could cause variation in a number of leaf traits.

Digital soil characteristics mapping for aiding site-specific management practices in the West Nile Delta, Egypt

Soil survey system is the key word for sustainable soil use and management. This study aimed to generate a digital soil mapping (DSM) using various spatial resolutions of freely available digital elevation models (DEMs) i.e., (30 m ALOS “Advanced Land Observing Satellite”, 12.5 m ALOS, 30 m SRTM “Shuttle Radar Topography Mission”, and 90 m SRTM) to assess the spatial variability of soil physio-chemical characteristics in El-Beheira Province, Western Nile Delta. A total of 196 geo-referenced soil profiles were collected from agricultural fields, and the soil's contents of total nitrogen (TN), potassium (K), available phosphorous (P), calcium (Ca), sulfur (S), magnesium (Mg), zinc (Zn), copper (Cu), manganese (Mn), and boron (B) were analyzed. The study revealed that the soil's contents of TN, P, S, B, and Cu were low, and Mg was suspected to be higher than K, leading to an Mg-induced K shortage. The study also found wide-ranging spatial variability structures of inverse distance weight (IDW) interpolated maps for several soil physio-chemical characteristics. The DSM further revealed that poor status was present in 85.4% of TN, 91.7% of P, 76.9% of S, and 87% of both B and Cu. The conclusion is that soil variability, as demonstrated in DSM, outweighs uniform treatment, and this discovery will assist extension workers, scientists, and decision-makers in implementing site-specific nutrient management strategies. Validation of the DSM is recommended for crop and variety-specific nutrient treatment rates. Understanding the geographical variance in the soil is critical for long-term nutrient management and higher output.

Effect of Tytanit on Selected Physiological Characteristics, Chemical Composition and Production of Festulolium braunii (K. Richt.) A. Camus

In intensive and sustainable agriculture, it is not enough to use plant protection products and fertilizers, but it is also important to control plant physiological processes. The aim of this study was to determine the effect of Tytanit on Festulolium braunii (K. Richt.) A. Camus dry matter yield, photosynthetic activity and the content of chlorophyll and selected chemical compounds. The pot experiment was conducted in 2019 in a plant breeding room. Four levels of treatment were used: control with no treatment and three stimulant concentrations of 0.02%, 0.04% and 0.06% in the spraying liquid. In particular, the research included the determination of dry weight of plant roots, dry weight of plants, chlorophyll a and b content in leaf blades, maximum and actual efficiency of the leaf photosystem, coefficients of non-photochemical and photochemical fluorescence quenching, and the content of total protein, crude fiber, monosaccharides, crude fat, crude ash, Ca, Mg, P and K in the dry matter of plants. Used in controlled conditions, the stimulant contributed to an increase in most parameter values, increasing photosynthetic activity and the content of chlorophyll a and b, total protein, calcium, magnesium and potassium, but it reduced the amounts of crude fiber.

Abstract 13409: Maintaining a High Total Serum Magnesium Level With Intravenous Magnesium Sulfate

Introduction: Understanding how intravenous magnesium sulfate (MgSO4) administration affects total serum magnesium concentration (TsMg) is important in maintaining a target TsMg level in acute heart failure patients on loop diuretics. Hypothesis: Describe how TsMg changes after single dose of MgSO4 and estimate how often MgSO4 should be given to maintain TsMg above 2 mg/dL. Methods: We studied hospitalizations of veterans from the Veterans Health Administration (VHA) aged ≥ 65 without severe renal disease (EGFR > 15 cc/min per 1.73 m2) from 2013-2017 with a primary diagnosis of acute heart failure. We constructed linear and logistic mixed-effects models with values of TsMg at level 1, nested within dose, hospitalization, and patient. Results: There were 27,474 doses of intravenous MgSO4 administered in 16,139 hospitalizations to 13,439 unique veterans across 145 hospitals in the VHA. Veterans were elderly (mean age 76), 98% male, 72% non-Hispanic white. Mean TsMg was initially high and leveled off 24-48 hours after MgSO4 administration, with an average TsMg increase of 0.2 mg/dL regardless of dose administered (Figure) . The probability of maintaining a TsMg above 2.0 mg/dL just one day after MgSO4 ranged from 49-54% (Table) . Conclusions: TsMg is often targeted above 2.0 gm/dL, especially in the setting of arrhythmias or QT-prolonging medications. As MgSO4 is frequently administered in heart failure patients on diuretics, understanding that TsMg may be misleadingly elevated for the first 48 hours after MgSO4 is important. We recommend administering MgSO4 at least once per day if the goal TsMg level is ≥ 2.0 gm/dL.

Effect of Improved Planting Stock on Tree Growth, Wood Properties, and Soil Fertility of Teak Plantations 10 Years After Planting

Teak (Tectona grandis Linn. f.) is one of the most economically valuable types of tropical forest because teak wood has high durability, resilience and good aesthetic qualities. Teak tree improvement in Indonesia was initiated in 1983 producing planting stock for reforestation, i.e. clonal seed orchards (CSO) to produce seedling as sexual reproduction (referred to seedling), and hedge orchards to produce shoot cuttings as asexual (vegetative) reproductive (referred to shoot cuttings). Teak plantations established using seedling and shoot cuttings should improve forest productivity. The objectives of this study were to compare the growth rate, wood properties, and soil fertility of teak plantations propagated using seedling and shoot cuttings. Three square plots of 100 trees (10 × 10 trees; 1,000 m2) were established on both types of plantation and tree growth characteristics, wood properties, and soil fertility were monitored. The tree growth characteristics were diameter at breast height (DBH), tree height (H), clear bole height (CB), and volume of standing stock (Vss), and the wood properties included pilodyn penetration (PP) and stress wave velocity (SWV). Soil fertility was assessed from soil samples collected from three soil depths, with three replicates in each plot: 0–5 cm (top layer), 6–30 cm (middle layer), and 31–60 cm (bottom layer). Soil samples were analysed for total nitrogen, total phosphorus, total potassium, total calcium, and total magnesium. The results showed that the mean DBH, H, CB, and Vss differed significantly between the type of planting stock. The DBH, H, CB, and Vss values were 28.4%, 46.8%, 49.1%, and 130.1% higher, respectively, in teak plantations from shoot cuttings than from seedling. Moreover, the SWV of shoot cuttings planting stock plantation was 3.6% higher than from seedling. The mean PP did not differ significantly between teak plantations from shoot cuttings (22.00 mm) and seedling (21.80 mm). Furthermore, the planting stock did not affect soil fertility, which was highest in the top layer in all treatments. Our results suggest that teak plantations from shoot cuttings would increase growth, forest productivity, maintaining wood quality and soil fertility. Thus, it can maintain sustainable teak forest plantations.

Ionized and Total Magnesium Levels and Cardiovascular Risk in Patients with CKD

Ionized and Total Magnesium Levels and Cardiovascular Risk in Patients with CKD

Magnesium intake and all-cause mortality after stroke: a cohort study

BackgroundPopulation-based studies have shown that adequate magnesium intake is associated with a lower risk of stroke and all-cause mortality. Whether adequate magnesium intake is important for reducing all-cause mortality risk after stroke remains unclear.MethodsWe analyzed data from 917 patients with a self-reported history of stroke from the National Health and Nutrition Examination Survey (NHANES) 2007–2018. The total magnesium intake was calculated by summing the magnesium intake from dietary and dietary supplements, and then adjusting for total energy intake according to the nutrient density method. Mortality status was determined using public-use linked mortality files from 2019. Cox regression model and restricted cubic splines were used to explore the relationship between magnesium intake and all-cause mortality.ResultsThe average total magnesium intake across all patients was 251.0 (184.5–336.5) mg/d, and 321 (70.2%) males and 339 (73.7%) females had insufficient magnesium intake. During a median follow-up period of 5.3 years, 277 deaths occurred. After fully adjusting for confounding factors, total magnesium intake levels were inversely associated with all-cause mortality risk (HR per 1-mg/(100 kcal*d) increase, 0.97; 95% CI, 0.94–1.00; p = 0.017). Participants with the highest quartile of total magnesium intake (≥ 18.5 mg/(100 kcal*d)) had a 40% reduction in all-cause mortality risk compared to those with the lowest quartile (≤ 12.0 mg/(100 kcal*d)) (HR, 0.60; 95% CI, 0.38–0.94; p = 0.024). Stratified analyses showed that this inverse association was statistically significant in those who were older, female, without hypertension, and had smoking, normal renal function, and adequate energy intake. Dietary magnesium intake alone might be not related to all-cause mortality.ConclusionsStroke survivors who consumed adequate amounts of magnesium from diet and supplements had a lower risk of all-cause mortality.

Effect of dietary supplementation of seaweeds on growth and blood profiles of TANUVAS Aseel chicken

The present study was conducted to evaluate the dietary effects of two seaweeds (Sargassum wightii and Gracilaria corticata) on different physiological parameters of TANUVAS Aseel chicken. A total of 30 TANUVAS Aseel chicks, day old were allocated into three groups (Each with 10 chicks) viz., one control group (C1) and two treatment groups (T1 and T2). The control group (C1) were fed with commercial grower feed alone and the treatment group (T1) had control diet with 5% Sargassum wightii and the treatment group (T2) had control diet mixed with 5% Gracilaria corticata. Parameters were recorded for a continuous period of 16 weeks in 4 weeks interval. The growth performance was found to be non-significant (P ≥ 0.05) during 0 day of feed trials and highly significant (P ≤ 0.01) during 4th, 8th, 12th and 16th week of feed trails. Haematological parameters during 12th and 16th week of feed trials showed non-significant (P ≥ 0.05) difference exist in lymphocyte, monocyte and eosinophil count and a highly significant (P ≤ 0.01) difference exist in platelets count between the control (C1) and treatment groups (T1 and T2). Serum biochemical parameters during 12th and 16th week of feed trials showed non-significant (P ≥ 0.05) difference in Uric acid, creatinine, ALT, calcium and cholesterol level and a highly significant (P ≤ 0.01) difference in Total protein, albumin, AST, glucose, triglycerides, phosphorus, magnesium, potassium and chloride level between the control (C1) and treatment groups (T1 and T2). However non-significant (P ≥ 0.05) difference exists in globulin and sodium content only during 16th week of feed trials. It could be noted that 5% inclusion of red seaweed, Gracilaria corticata in commercial grower feed as a feed supplement to TANUVAS Aseel chicks had a better body weight gain than 5% inclusion level of brown seaweed, Sargassum wightii in commercial grower feed. Based on the haematological and serum biochemical analysis, the supplementation of 5% inclusion level of seaweeds in this trial did not pose any threat to the physiological well-being of TANUVAS Aseel chicken.

Incidence of Dysmagnesemia among Medically Hospitalized Patients and Associated Clinical Characteristics: A Prospective Cohort Study.

Magnesium (Mg) disorders are common among hospitalized patients and are linked to poor health outcomes. We aimed to determine the incidence of dysmagnesemia among medically hospitalized patients and to identify factors that are associated with dysmagnesemia. A prospective cohort study was conducted at Sultan Qaboos University Hospital (SQUH) from April 1st, 2022, to October 31st, 2022, and involved hospitalized adult patients (≥18 years) under the care of the general internal medicine unit. The patients' serum total magnesium (Mg) concentrations were categorized as hypomagnesemia (≤0.69 mmol/L), hypermagnesemia (≥1.01 mmol/L), or dysmagnesemia, which encompassed either hypomagnesemia or hypermagnesemia. Of the 304 patients evaluated, dysmagnesemia was observed in 22.0%, which comprised of 17.4% with hypomagnesemia and 4.6% with hypermagnesemia. Statistically significant associations were identified between hypermagnesemia and chronic kidney disease (CKD) (p = 0.05) and elevated creatinine levels (p < 0.01) and lower estimated glomerular filtration rate (eGFR) (p < 0.01). Hypomagnesemia was linked to lower ionized calcium (p = 0.03) and admission due to infectious diseases (p = 0.02). However, ordered regression analysis did not find any significant associations with the different magnesium groups. Dysmagnesemia was prevalent among hospitalized patients and was associated with different factors; however, ordered regression analysis did not find any association with the different magnesium group, probably due to the limited number of included individuals.

Impact of Automobile Workshop Activities on Groundwater in Aule, Ondo State, Nigeria

In many parts of Nigeria, groundwater is one of the most essential sources of water. Pollution brought on by petroleum products (a complete mixture of hydrocarbons) has been acknowledged as a severe environmental issue. This research is aimed at examining the impact of automobile repair activities on well water quality in Aule, Ondo state, Nigeria. Physio-chemical parameters such as temperature, turbidity, pH, electrical conductivity, total dissolved solids, alkalinity, acidity, total hardness, chloride, nitrate, sulphate, phosphate, total suspended solids, magnesium, calcium and bacteriological parameters from 5 wells in different automobile workshops and a control well in Aule were analyzed in this research. The results were compared with the WHO standards. The analysis revealed that the mean values of NO3, SO4, Mg, Ca, and pH were within the WHO permissible limit, while the mean value of the other water parameters exceeded the WHO permissible limit. Also, NO3 negatively correlated pH, and positively with Ca (r=0.873, p&lt;0.05). PO4 correlated positively with Ca (r=0.903, p&lt;0.05). TDS correlated negatively with Temp, and pH and positively with TSS (r=0.879, p&lt;0.05). pH correlated negatively with EC, pH, and TVBC. This study also revealed that poor waste disposal practices by automobile repairers, inadequate regulatory frameworks, and a lack of awareness among community members on the dangers of groundwater pollution are contributing factors to the problem.

Floating Constructed Wetlands Efficiency in Removal of Total Hardness, Calcium and Magnesium from Secondary Treated Sewage Water

Floating Constructed wetlands (FCWs) also popular as Floating Treatment Wetlands (FTWs) were used in situ for suspended solids,heavy metals, biological oxygen demand (BOD), chemical oxygen demand (COD), faecal coliform bacteria, nutrients and heavy metalsremediation from various types of wastewater. However, there was limited data available on total hardness (TH), calcium (Ca2+) andmagnesium (Mg2+) ions removal from secondary treated sewage water (STSW). This article focuses on TH, Ca2+ and Mg2+ ions removalefficiency of FCWs from STSW. Mesocosms include a control (without FCWs), FCWs planted with macrophyte plant species Cannaindica var. indica L. (S1) and Typha angustifolia L. (S2) and one mixed culture with both Typha and Canna plants (S3). The changes in TH,Ca2+ and Mg2+ concentration in wastewater were recorded at a 7-day hydraulic retention time (HRT) for 7 batches. Mean percentageremoval efficiency recorded was 9.8, 1.7, 4.9 and 11.2% for TH, -3.4, 7.6, 27.1 and 14.1% for Ca2+and 29.5, 4.2, -0.6, and -0.6% for Mg2+,in Control, S1, S2, S3 FCWs respectively. Results showed that S2 and S3 FCWs are effective in TH and Ca2+ removal but not for Mg2+ ions.Thus, FCWs can reduce TH and Ca2+ ions concentration along with nutrients from STSW.

A-112 A Consensus Reference Range for Ionized Magnesium

Abstract Objective Ionized magnesium (iMg) is the physiologically active form of the analyte. Despite this, clinical adoption of iMg is infrequent, and there is only one commercially available analyzer that measures iMg. There are now numerous studies that have examined iMg levels in healthy individuals, and this report will summarize their findings and report a consensus reference range. Relevance Although reference ranges may vary based on population and location, among other factors, a baseline reference range is an important starting point to introducing a new test to any laboratory. Methods A literature search was performed using the search term “ionized magnesium”. Papers with reference ranges for healthy individuals measured by ion-selective electrodes were selected and an attempt was made to establish a reference range for adults and children. Results A total of 10 studies were identified, with 422 healthy individuals [1–10]. There were 322 adults and 100 children. Studies looked at venous samples and serum samples. Venous samples were anticoagulated with lithium heparin.Reference ranges for adults and children are shown below: To convert from mg/dL to mmol/L multiply by 0.41. To convert from mg/dL to MEq/L multiply by 1.22. Conclusions Although a large-scale study would be helpful in determining a more precise reference range, there is reasonably good agreement among studies evaluating iMg reference ranges in healthy adults and children. Further work to evaluate differences among different age groups, genders, and ethnic groups would be helpful to clinicians and researchers. There has been a call for updating reference ranges for total magnesium, as there is a wide variation in reported ranges [11].

Nonlinear relationship between dietary calcium and magnesium intake and peripheral neuropathy in the general population of the United States.

Calcium and magnesium are essential minerals that have significant roles in nerve function and regulation. There may be a correlation between dietary calcium and magnesium intake and peripheral neuropathy. However, this relationship remains unclear and requires further study. Data from 7,726 participants in the National Health and Nutrition Examination Survey (NHANES) from 1999 to 2004 were analyzed in this study. The relationship between total dietary calcium and magnesium intake, as well as each quantile, and peripheral neuropathy was analyzed using a multifactor logistic regression model. To illustrate the dose-response relationship between calcium and magnesium intake and peripheral neuropathy, we utilized a restricted cubic spline (RCS) plot. Our analysis found a positive correlation between dietary intake of calcium and magnesium and peripheral neuropathy (calcium: OR 1.000, 95% CI 1.000-1.000; magnesium: OR 1.001, 95% CI 1.00-1.002). Participants in the first and third quantiles of dietary calcium intake had a significantly higher incidence of peripheral neuropathy than those in the second quantile (OR 1.333, 95% CI 1.034-1.719, OR 1.497, 95% CI 1.155-1.941). Those in the first and third quantiles of dietary magnesium intake also had a significantly higher incidence of peripheral neuropathy than those in the second quantile (OR 1.275, 95% CI 1.064-1.528, OR 1.525, 95% CI 1.231-1.890). The restricted cubic spline analysis revealed a U-shaped nonlinear relationship between dietary intake of calcium and magnesium and peripheral neuropathy. The study found a U-shaped non-linear relationship between dietary calcium and magnesium intake levels and peripheral neuropathy, indicating that both excessive and insufficient intake of calcium and magnesium can increase the incidence of peripheral neuropathy.

Loss of CorA, the primary magnesium transporter of Salmonella, is alleviated by MgtA and PhoP-dependent compensatory mechanisms.

In many Gram-negative bacteria, the stress sigma factor of RNA polymerase, σS/RpoS, remodels global gene expression to reshape the physiology of stationary phase cells and ensure their survival under non-optimal growth conditions. In the foodborne pathogen Salmonella enterica serovar Typhimurium, σS is also required for biofilm formation and virulence. We have recently shown that a ΔrpoS mutation decreases the magnesium content and expression level of the housekeeping Mg2+-transporter CorA in stationary phase Salmonella. The other two Mg2+-transporters of Salmonella are encoded by the PhoP-activated mgtA and mgtB genes and are expressed under magnesium starvation. The σS control of corA prompted us to evaluate the impact of CorA in stationary phase Salmonella cells, by using global and analytical proteomic analyses and physiological assays. The ΔcorA mutation conferred a competitive disadvantage to exit from stationary phase, and slightly impaired motility, but had no effect on total and free cellular magnesium contents. In contrast to the wild-type strain, the ΔcorA mutant produced MgtA, but not MgtB, in the presence of high extracellular magnesium concentration. Under these conditions, MgtA production in the ΔcorA mutant did not require PhoP. Consistently, a ΔmgtA, but not a ΔphoP, mutation slightly reduced the magnesium content of the ΔcorA mutant. Synthetic phenotypes were observed when the ΔphoP and ΔcorA mutations were combined, including a strong reduction in growth and motility, independently of the extracellular magnesium concentration. The abundance of several proteins involved in flagella formation, chemotaxis and secretion was lowered by the ΔcorA and ΔphoP mutations in combination, but not alone. These findings unravel the importance of PhoP-dependent functions in the absence of CorA when magnesium is sufficient. Altogether, our data pinpoint a regulatory network, where the absence of CorA is sensed by the cell and compensated by MgtA and PhoP- dependent mechanisms.

Multi-ahead electrical conductivity forecasting of surface water based on machine learning algorithms

The present research work focused on predicting the electrical conductivity (EC) of surface water in the Upper Ganga basin using four machine learning algorithms: multilayer perceptron (MLP), co-adaptive neuro-fuzzy inference system (CANFIS), random forest (RF), and decision tree (DT). The study also utilized the gamma test for selecting appropriate input and output combinations. The results of the gamma test revealed that total hardness (TH), magnesium (Mg), and chloride (Cl) parameters were suitable input variables for EC prediction. The performance of the models was evaluated using statistical indices such as Percent Bias (PBIAS), correlation coefficient (R), Willmott’s index of agreement (WI), Index of Agreement (PI), root mean square error (RMSE) and Legate-McCabe Index (LMI). Comparing the results of the EC models using these statistical indices, it was observed that the RF model outperformed the other algorithms. During the training period, the RF algorithm has a small positive bias (PBIAS = 0.11) and achieves a high correlation with the observed values (R = 0.956). Additionally, it shows a low RMSE value (360.42), a relatively good coefficient of efficiency (CE = 0.932), PI (0.083), WI (0.908) and LMI (0.083). However, during the testing period, the algorithm’s performance shows a small negative bias (PBIAS = − 0.46) and a good correlation (R = 0.929). The RMSE value decreases significantly (26.57), indicating better accuracy, the coefficient of efficiency remains high (CE = 0.915), PI (0.033), WI (0.965) and LMI (− 0.028). Similarly, the performance of the RF algorithm during the training and testing periods in Prayagraj. During the training period, the RF algorithm shows a PBIAS of 0.50, indicating a small positive bias. It achieves an RMSE of 368.3, R of 0.909, CE of 0.872, PI of 0.015, WI of 0.921, and LMI of 0.083. During the testing period, the RF algorithm demonstrates a slight negative bias with a PBIAS of − 0.06. The RMSE reduces significantly to 24.1, indicating improved accuracy. The algorithm maintains a high correlation (R = 0.903) and a good coefficient of efficiency (CE = 0.878). The index of agreement (PI) increases to 0.035, suggesting a better fit. The WI is 0.960, indicating high accuracy compared to the mean value, while the LMI decreases slightly to − 0.038. Based on the comparative results of the machine learning algorithms, it was concluded that RF performed better than DT, CANFIS, and MLP. The study recommended using the current month’s total hardness (TH), magnesium (Mg), and chloride (Cl) parameters as input variables for multi-ahead forecasting of electrical conductivity (ECt+1, ECt+2, and ECt+3) in future studies in the Upper Ganga basin. The findings also indicated that RF and DT models had superior performance compared to MLP and CANFIS models. These models can be applied for multi-ahead forecasting of monthly electrical conductivity at both Varanasi and Prayagraj stations in the Upper Ganga basin.

Ionized Magnesium Correlates With Total Blood Magnesium in Pediatric Patients Following Kidney Transplant.

Abnormal serum magnesium (Mg) concentrations are common and associated with worse mortality in kidney-transplant recipients. Many kidney and transplant-related factors affect Mg homeostasis. The concentration of the active form, ionized Mg (iMg), is not measured clinically, and total Mg (tMg) and iMg correlations have conflicted. We hypothesized that iMg and tMg concentrations show poor categorical agreement (i.e., low, normal, and high) in kidney-transplant recipients but that ionized calcium (iCa) correlates with iMg. We retrospectively evaluated hypomagnesemia in kidney-transplant recipients over a 2-yr period. We prospectively collected blood at 0-28 days post-transplant to measure correlations between iMg and iCa/tMg. iMg and iCa concentrations in the reference ranges of 0.44-0.65 and 1.0-1.3 mmol/L, respectively, were considered normal. Fisher's exact test and unweighted kappa statistics revealed category agreements. Pearson's correlation coefficients and linear regression measured correlations. Among 58 retrospective kidney-transplant recipients, 54 (93%) had tMg<0.66 mmol/L, 28/58 (48%) received Mg supplementation, and 20/28 (71%) had tacrolimus dose adjustments during supplementation. In 13 prospective transplant recipients (N=43 samples), iMg and tMg showed strong category agreement (P=0.0003) and correlation (r=0.71, P<0.001), whereas iMg and iCa did not (P=0.7; r=-0.25, P=0.103, respectively). tMg and iMg exhibited strong correlation following kidney transplantation. However, iCa may not be an accurate surrogate for iMg. Determining the effect of Mg supplementation and the Mg concentration where supplementation is clinically necessary are important next steps.