By constructing a rural digital design ecosystem, this paper develops ecological villages through design empowerment, enhances the carbon sequestration benefits of plants in rural areas, and strengthens rural vitality. Combined with the carbon sequestration benefits of street trees in Bulao Village in Nanjing, the feasibility of the digital design ecosystem in rural planning was verified, and the ways and methods of rural environmental renewal were explored. Through the existing literature, the possibility of constructing a digital design ecosystem was deduced, the theoretical framework was derived, field research was carried out in the village of Bulao, the carbon sequestration benefit of street trees was quantified by the i-Tree model, and a structure chart of street trees, including breast diameter, tree height, type, etc., was formed. There were 35 species of street trees in Bulao Village, belonging to 33 genera in 22 families, including 19 species of trees, a total of 312 trees, and 16 species of shrubs. The street trees’ total carbon sink benefit was equivalent to RMB 30,327.47, a single street tree’s average carbon sequestration benefit was RMB 96.86, and the average CO2 absorption was 164.64 kg. The average CO2 absorption and single benefit of elm trees were the highest, reaching 465.48 kg·plant−1 and 186.81 RMB·plant−1, respectively. The CO2 absorption (185.13 kg) and the average benefit per plant (RMB 109.48) of the camphor tree were lower than those of the elm. However, because their number far exceeded that of elms, their total carbon sequestration benefit contribution was the highest, reaching RMB 25,837.28, accounting for 85.19% of the total benefit. In addition, the contribution rates of elm and willow’s total annual carbon sequestration benefits were also relatively high, reaching RMB 747.24 and RMB 710.04, respectively, accounting for 2.46% and 2.34% of the total benefits. This paper uses the digital design ecosystem’s theoretical framework to quantify street trees’ carbon sequestration benefits through field research. It optimizes and improves the plant allocation of parking lots in Bulao Village from the ecology and carbon sink perspectives. Practice shows that inheriting the connotation values of rural culture, improving the quality of the rural environment, and increasing residents’ and tourists’ sense of belonging and identity to the countryside are conducive to jointly promoting sustainable rural development against the background of “dual carbon”. Combining art design with quantitative scientific methods of ecological environment indicators provides a reference for future rural development.