Topographic Images Research Articles

Effects of curcumin in the interaction with cardiolipin-containg lipid monolayers and bilayers

Curcumin, a plant polyphenol extracted from the Chinese herb turmeric, has gained widespread attention in recent years because of its multifunctional properties as antioxidant, antinflammatory, antimicrobial, and anticancer agent. Effects of the molecule on mitochondrial membranes properties have also been evidenced. In this work, the interaction of curcumin with models of mitochondrial membranes composed of dimyristoylphosphatidylcholine (DMPC) or mixtures of DMPC and 4 mol% tetramyristoylcardiolipin (TMCL) has been investigated by using biophysical techniques. Spectrophotometry and fluorescence allowed to determine the association constant and the binding energy of curcumin with pure DMPC and mixed DMPC/TMCL aqueous bilayers. The molecular organization of pure DMPC and cardiolipin-containing Langmuir monolayers at the air-water interface were investigated and the morphology of the monolayers transferred into mica substrates were characterized through atomic force microscopy (AFM). It is found that curcumin associates at the polar/apolar interface of the lipid bilayers and the binding is favored in the presence of cardiolipin. At 2 mol%, curcumin is well miscible with lipid monolayers, particularly with mixed DMPC/TMCL ones, where compact terraces formation characterized by a reduction of the surface roughness is observed in the AFM topographic images. At 10 mol%, curcumin perturbs the stability of DMPC monolayers and morphologically are evident terraces surrounded by cur aggregates. In the presence of TMCL, very few curcumin aggregates and larger compact terraces are observed. The overall results indicate that cardiolipin augments the incorporation of curcumin in model membranes highlighting the mutual interplay cardiolipin-curcumin in mitochondrial membranes.

Probe conditioning via convolution neural network for scanning probe microscopy automation

We present an automation system for conditioning a scanning probe microscopy (SPM) probe into different states on a Si(111)–(7 × 7) surface at room temperature. Topography images representing multiple surface states and probe condition states divided into 11 categories and trained by a convolution neural network with an accuracy of 87% were used to estimate the effectiveness of the probe with an accuracy of 98%. We demonstrate the responsiveness of the method by experimentally reforming a probe into different conditions defined by preset categories. This system will promote advancements in autonomous SPM experiments at atomic scale and room temperature.

Portable confocal-controlled LIBS microscopy for topographic and LIBS mapping

The on-site detection of the geometrical topography and composition distribution of unknown minerals is a key bottleneck in the field of deep space exploration and geological material analysis. A novel portable confocal-controlled laser-induced breakdown spectroscopy (PCCLIBS) scanning three-dimensional (3D) imaging method with high spatial resolution and highly integrated structure was proposed to solve this problem. The method utilizes a confocal-controlled LIBS technique to achieve precise focusing of the sample, significantly improving the spatial resolution of the LIBS mapping system. In addition, we employ a dual two-dimensional (2D) MEMS mirror scanning technique to achieve a miniaturized design of LIBS mapping system. These two technologies are combined to achieve miniaturization, high spatial resolution, and 3D LIBS microelement imaging. The method had an axial focusing ability of ∼ 60 nm and a lateral resolution of geometrical and elemental maps of around 700 nm and 10 μm, respectively, using an objective lens with a numerical aperture (NA) of 0.4. Finally, we performed the LIBS and topographic imaging of carbonaceous chondrite (Northwest Africa 13323) using the PCCLIBS system to realize multi-information of the micro-regional chemical, element, and topographic information imaging in the sample. These results demonstrate that this method is an effective approach for the elemental characterization of LIBS in samples with complex morphologies, such as in planet landing exploration and geological exploration.

In situ plasmonic tip preparation and validation techniques for scanning tunneling microscopy

Among the many parts constituting a scanning tunneling microscope, the metallic tip is the component that directly interacts with the specimen and plays a critical role in visualizing the physical quantity of interest. While tip materials such as W and Pt–Ir are commonly used for topographic imaging and their preparation is well-documented, the preparation of plasmonic materials such as Ag for tip-enhanced Raman spectroscopy is relatively less standardized. Here, we present several in situ Ag tip preparation and validation techniques for the microscopist to use depending on their intended application, including atomic resolution imaging, scanning tunneling spectroscopy (STM), and tip-enhanced Raman spectro-microscopy in ultrahigh vacuum. Besides optical applications, these methods are not limited to Ag but also applicable to other STM tip materials.

Airborne UAV Remote Sensor Position Accuracy Algorithm in Catastrophes Zones

Sensor payload calibration and FOV are still the most essential parameters in Airborne Remote Sensors for developing quality terrain images in the Remote Sensing field. UAVs are core devices in Airborne Remote Sensing by carrying camera payload for large terrain objects to achieve desired image quality with geographical position provided for different available flight planning algorithms.
 This paper proposes the algorithm to find the exact Geo-coordinates for UAV navigation algorithm viewpoint identification for implementing Airborne Remote Sensing (ARS) to study Landslide Susceptibility Zone as well as application for catastrophe analysis. Terrain Slope and Aspect was considered using Remote Sensing products from ISRO CartoSat and NASA ASTER satellite available in the Digital Elevation Model (DEM) construction to maintain the calibrated angle of vision, field of vision, and land surface image quality.
 This algorithm will enhance the functioning of various available UAV Flight Planning Algorithms to improve the quality of 3D topography images for 3D Modeling as well as Landslide and Flood Prediction and Analysis, as well as provide the position of camera payload and tilting angle for enhanced terrain images.

Three-Dimensional Light Field Fundus Imaging: Automatic Determination of Diagnostically Relevant Optic Nerve Head Parameters.

Morphological changes to the optic nerve head (ONH) can be detected at the early stages of glaucoma. Three-dimensional imaging and analysis may aid in the diagnosis. Light field (LF) fundus cameras can generate three-dimensional (3D) images of optic disc topography from a single shot and are less susceptible to motion artifacts. Here, we introduce a processing method to determine diagnostically relevant ONH parameters automatically and present the results of a subject study performed to validate this method. The ONHs of 17 healthy subjects were examined and images were acquired with both an LF fundus camera and by optical coherence tomography (OCT). The LF data were analyzed with a novel algorithm and compared with the results of the OCT study. Depth information was reconstructed, and a model with radial basis functions was used for processing of the 3D point cloud and to provide a finite surface. The peripapillary rising and falling edges were evaluated to determine optic disc and cup contours and finally calculate the parameters. Nine of the 17 subjects exhibited prominent optic cups. The contours and ONH parameters determined by an analysis of LF 3D imaging largely agreed with the data obtained from OCT. The median disc areas, cup areas, and cup depths differed by 0.17mm², -0.04 mm², and -0.07 mm, respectively. The findings presented here suggest the possibility of using LF data to evaluate the ONH. LF data can be used to determine geometric parameters of the ONH and thus may be suitable for future use in glaucoma diagnostics.

How to Choose the Right AFM Probe for Your Experiment

Abstract Atomic force microscopes (AFMs) have emerged as the principal enabling tool for nanotechnology research. They are used ubiquitously in a wide range of fields: from 2D materials, semiconductors, ferroelectrics, and batteries to biomolecules, polymers, and cell biology. As the name implies, AFMs are microscopes. However, rather than using focused light or electrons to magnify sample features, AFMs scan a mechanical probe with a very sharp tip over the surface to create a high-resolution 3D topographical image. Further, by modifying the probe composition or structure, other material properties (electrical, mechanical, magnetic, etc.) can be simultaneously measured and mapped onto the topographic image for precise structure/property correlation. Clearly, the probe is key to unlocking the power of the AFM, thus, choosing the right probe is critical. In this article, we will provide novice and experienced users with basic information and guidelines to simplify the AFM probe selection process.

Influence of structural defects on charge density waves in 1T-TaS2

The influence of intrinsic defects of 1T-TaS2 on charge density waves (CDWs) is studied using scanning tunneling microscopy and spectroscopy (STM, STS), angle-resolved photoelectron spectroscopy (ARPES), and density functional theory (DFT). We identify several types of structural defects and find that most have a local character limited to a single CDW site, with a single exception which effectively behaves as a dopant, leading to band-bending and affecting multiple neighboring sites. While only one type of defect can be observed by STM topographic imaging, all defects are easily resolved in STS mapping. Our results indicate modulation of the Mott band gap commensurate with the CDW and breaking of the three-fold symmetry of electronic states. DFT calculations (with included Coulomb interactions) are used to investigate the electronic structure, focusing on both sulfur vacancy and oxygen-sulfur substitution. The sulfur vacancy system, characterized with a metallic behavior, is identified as the origin of one of the experimentally observed defects. Additionally, the effect of oxidation of 1T-TaS2 depends on the substitution site, leading to the heterogeneity of electronic properties.

Geophysically guided well siting at the Aluto-Langano geothermal reservoir

Volcano-hosted high-temperature geothermal reservoirs are powerful resources for green electricity generation. In regions where such resources are available, geothermal energy often provides a large share of a country’s total power generation capacity. Sustainable geothermal energy utilization depends on the successful siting of geothermal wells, which in turn depends on prior geophysical subsurface imaging and reservoir characterization. Electromagnetic resistivity imaging methods have proven to be a key tool for characterizing magma-driven geothermal systems because resistivity is sensitive to the presence of melt and clays that form through hydrothermal alteration. Special emphasis is often given to the “clay cap,” which forms on top of hydrothermal reservoirs along the flow paths of convecting geothermal fluids. As an example, the Aluto-Langano volcanic geothermal field in Ethiopia is covered with 178 densely spaced magnetotelluric (MT) stations. The 3D electrical conductivity model derived from the MT data images the magma body that acts as a heat source of the geothermal system, controlling geothermal convection and formation of alteration zones (commonly referred to as clay cap) atop the geothermal reservoir. Detailed 3D imaging of the clay cap topography can provide direct insight into hydrothermal flow patterns and help identify potential “upflow” zones. At Aluto all productive geothermal wells are drilled into zones of clay cap thinning and updoming, which is indicative of underlying hydrothermal upflow zones. In contrast, nonproductive wells are drilled into zones of clay cap thickening and lowering, which is an indicator for underlying “outflow” zones and cooling. This observation is linked to fundamental characteristics of volcano-hosted systems and can likely be adapted to other geothermal fields where sufficiently detailed MT surveys are available. Therefore, high-resolution 3D electromagnetic imaging of hydrothermal alteration products (clay caps) can be used to infer the hydrothermal flow patterns in geothermal reservoirs and contribute to derisking geothermal drilling projects.

Nanostructures and Thin Films of Poly(Ethylene Glycol)-Based Surfactants and Polystyrene Nanocolloid Particles on Mica: An Atomic Force Microscopy Study

We studied the nanostructures and ultrathin films resulting from the deposition and adsorption of polystyrene nanocolloidal particles and methoxy poly(ethylene glycol) methacrylate surfactants on mica surfaces from mixed suspensions in water. The samples were prepared by droplet evaporation and dip coating and imaged with atomic force microscopy. Topography and phase imaging revealed a significant richness in morphological features of the deposited/adsorbed films. We observed uniform ultrathin films and extended islands of the surfactant oligomers indicating their self-assembly in monolayers and multilayers, while the polystyrene nanocolloids were embedded within the surfactant structures. Droplet evaporation resulted in the migration of particles towards the edges of the droplet leaving an intricate network of imprints within the surfactant film. Dip coating induced the formation of extended nanocolloid clusters with colloidal crystalline structuring.

Scanning ion conductance microscope with a capacitance-compensated current source amplifier.

A high-speed imaging method for a scanning ion conductance microscope (SICM) based on a current source amplifier that compensates for unavoidable capacitance is proposed. The capacitance is generated on a side wall of a nanopipette in the principle of the SICM. The electrical response time is deteriorated due to the capacitance, and the probe overshoots the setpoint of the detection of the sample surface. A capacitance compensation circuit was installed in a feedback circuit of the current source amplifier. The proposed capacitance compensation method is useful because it can shorten the imaging time by only installing the compensation circuit in the ion current detection circuit of an existing SICM. The maximum approaching speeds with and without capacitance compensation were found to be 1050 and 450 µm/s, respectively. The approaching speed with capacitance compensation was 2.3 times faster than that without capacitance compensation. A topographic image of the test sample was successfully obtained at an approaching speed of 1050 µm/s. The images of microvillus dynamics of COS-7 cells were obtained at ∼23.4 s/frame as an application of the developed technology.

A one-dimensional temperature and age modeling study for selecting the drill site of the oldest ice core near Dome Fuji, Antarctica

Abstract. The recovery of a new Antarctic ice core spanning the past ∼ 1.5 million years will advance our understanding of climate system dynamics during the Quaternary. Recently, glaciological field surveys have been conducted to select the most suitable core location near Dome Fuji (DF), Antarctica. Specifically, ground-based radar-echo soundings have been used to acquire highly detailed images of bedrock topography and internal ice layers. In this study, we use a one-dimensional (1-D) ice-flow model to compute the temporal evolutions of age and temperature, in which the ice flow is linked with not only transient climate forcing associated with past glacial–interglacial cycles but also transient basal melting diagnosed along the evolving temperature profile. We investigated the influence of ice thickness, accumulation rate, and geothermal heat flux on the age and temperature profiles. The model was constrained by the observed temperature and age profiles reconstructed from the DF ice-core analysis. The results of sensitivity experiments indicate that ice thickness is the most crucial parameter influencing the computed age of the ice because it is critical to the history of basal temperature and basal melting, which can eliminate old ice. The 1-D model was applied to a 54 km long transect in the vicinity of DF and compared with radargram data. We found that the basal age of the ice is mostly controlled by the local ice thickness, demonstrating the importance of high-spatial-resolution surveys of bedrock topography for selecting ice-core drilling sites.

Investigation on Metal Hybrid Fibres Laminate (MHFL) in hybrid machining

Metal hybrid fibre laminates (MHFLs) are gaining extensive attention due to their low weight-high strength and superior durability, which are used for primary constructions in a comprehensive application of aerospace and automobile industries. However, machining intricate shapes in MHFL is a challenge ahead of conventional machining processes. Hence, the present work aims at the machinability study of Titanium metal cored basalt/flax fibre laminate by using ultrasonic frequency vibration coupled wire electrical discharge machining (UFV-WEDM). The process- variables such as pulse on (Ton), pulse off (Toff), current (I), cutting angle (CA), and servo voltage (SV) coupled with ultrasonic frequency vibration (UFV) are varied at experimentation designed by using the Central Composite Design (CCD). The machined surface is characterized by the material removal rate (MRR) and surface roughness (SR). The empirical findings show that the servo voltage significantly influences MRR (73.93 %) and SR (70.02 %). The performance sequence order of significant influencing parameters is SV > Toff>CA > Ton>I. The Desirability analysis generated the optimum setting for minimizing the SR & maximizing MRR is Ton = 16 μs, Toff =14 μs, SV = 70 V, I = 3 A and CA = 30°. Scanning Electron Microscopic, 3D topography and atomic force microscopic (AFM) images were used to perform the micro-analysis on the machined surface of MHFL.

An Approach for 3D Modeling of the Regular Relief Surface Topography Formed by a Ball Burnishing Process Using 2D Images and Measured Profilograms.

Advanced in the present paper is an innovative approach for three-dimensional modeling of the regular relief topography formed via a ball burnishing process. The proposed methodology involves capturing a greyscale image of and profile measuring the surface topography in two perpendicular directions using a stylus method. A specially developed algorithm further identifies the best match between the measured profile segment and a row or column from the captured topography image by carrying out a signal correlation assessment based on an appropriate similarity metric. To ensure accurate scaling, the image pixel grey levels are scaled with a factor calculated as being the larger ratio between the ultimate heights of the measured profilograms and the more perfectly matched image row/column. Nine different similarity metrics were tested to determine the best performing model. The developed approach was evaluated for eight distinct types of fully and partially regular reliefs, and the results reveal that the best-scaled 3D topography models are produced for the fully regular reliefs with much greater heights. Following a thorough analysis of the results obtained, at the end of the paper, we draw some conclusions and discuss potential future work.

Observation of skyrmion bubbles in multilayer [Pt/Co/Cu]n using spin-polarized STM

Magnetic multilayers are a promising platform for storage and logic devices based on skyrmion spin textures, due to the large materials phase space for tuning properties. Epitaxial superlattice structures of [Pt/Co/Cu]n thin films were grown by molecular beam epitaxy at room temperature. Spin-polarized scanning tunneling microscopy (SP-STM) of these samples was used to probe the connection between surface structure and skyrmion morphology with nanoscale spatial resolution. Irregular-shaped skyrmion bubbles were observed, with effective diameters from 20 to 200 nm that are much larger than the nanoscale grain structure of the surface topography. Nucleation, annihilation, and motion of skyrmion bubbles could be driven using the stray field of the ferromagnetic tip in repeated imaging, and spin-polarized current/voltage pulses. Our detailed comparison of STM topography and differential conductance images shows that there are no surface defects or inhomogeneities at length scales that could account for the range in skyrmion bubble size or shape observed in the measurements.

Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection.

An Atomic Force Microscope(AFM) isa powerful and versatile tool for nanoscale surface studies to capture 3D topography images of samples. However, due to their limited imaging throughput, AFMs have not been widely adopted for large-scale inspection purposes. Researchers have developed high-speed AFM systems to record dynamic process videos in chemical and biological reactions at tens of frames per second, at the cost of a small imaging area of up to several square micrometers. In contrast, inspecting large-scale nanofabricated structures, such as semiconductor wafers, requires nanoscale spatial resolution imaging of a static sample over hundreds of square centimeters with high productivity. Conventional AFMs use a single passive cantilever probe with an optical beam deflection system, which can only collect one pixel at a time during AFM imaging, resulting in low imaging throughput. This work utilizes an array of active cantilevers with embedded piezoresistive sensors and thermomechanical actuators, which allows simultaneous multi-cantilever operation in parallel operation for increased imaging throughput. When combined with large-range nano-positioners and proper control algorithms, each cantilever can be individually controlled to capture multiple AFM images. With data-driven post-processing algorithms, the images can be stitched together, and defect detection can be performed by comparing them to the desired geometry. This paper introduces principles of the custom AFM using the active cantilever arrays, followed by a discussion on practical experiment considerations for inspection applications. Selected example images of silicon calibration grating, highly-oriented pyrolytic graphite, and extreme ultraviolet lithography masks are captured using an array of four active cantilevers ("Quattro") with a 125 µm tip separation distance. With more engineering integration, this high-throughput, large-scale imaging tool can provide 3D metrological data for extreme ultraviolet (EUV) masks, chemical mechanical planarization (CMP) inspection, failure analysis, displays, thin-film step measurements, roughness measurement dies, and laser-engraved dry gas seal grooves.

Inner-paddled atomic force microscopy cantilever for rapid mechanical mapping

Mechanical characterization methods at the nanoscale are of critical importance for many fields including nanomaterials, micro/nano devices and nanomechanics. As a key tool in nanotechnology, atomic force microscopy (AFM) is widely used due to its high-resolution topography imaging capabilities, and is also recognized as a useful platform for nanoscale mechanical characterization. Contact-resonance AFM, which modulates the tip-sample contact with ultrasonic frequencies and then analyzes the cantilever’s resonance responses, is an important AFM method for viscoelastic characterization. However, contact-resonance AFM requires the cantilever’s contact-resonance frequency and quality factor values to quantify the elastic modulus and loss tangent of the sample. This requires time-consuming frequency sweep, which makes a quantitative scanning impractical, therefore only single-point quantitative measurement and qualitative single-frequency scanning are usually applied. To address this issue, here we present an AFM cantilever design with an integrated inner-paddle substructure, which provides an eigenmode whose resonance keeps consistent, but whose resonance amplitude varies with varying contact stiffness. With this probe, it is hoped to quantify the elastic properties of the sample with fast single-frequency amplitude imaging.

Formation and propagation mechanism of complex stacking fault in 180 μm thick 4H-SiC epitaxial layers

The formation mechanism and propagation behavior of a complex stacking fault (SF) in thick silicon carbide epitaxial layers was investigated using depth-resolved section X-ray topography and ultraviolet photoluminescence (UVPL) imaging. The SF is initiated in the substrate by deflection of a threading screw dislocation onto two basal half planes creating a Frank fault. This fault propagates into the epitaxial layer and interacts with several threading mixed dislocations, which creates both Frank and Shockley SFs on the same basal plane. UVPL images using different spectral filters show various SF type conversions occurring during propagation of the complex SF.

Effective Penetration Depth Investigation for Frank Type Dislocation (Deflected TSDs/TMDs) on Grazing Incidence Synchrotron X-Ray Topographs of 4H-SiC Wafers

In 4H-SiC crystals, Frank type dislocations are created through the deflection of threading screw/mixed dislocations onto the basal plane. Grazing-incidence X-ray topographs are often used to evaluate the density of such dislocations and a knowledge of the effective penetration depth is therefore essential. In this study, a systematic analysis is performed to investigate the effective penetration depth, which is the depth from which contrast from the dislocation is still discernible. This is achieved by comparison between observed topographic images and detailed ray tracing simulations. Simulations shows no significant contrast difference between a deflected TSD and a deflected TMD with the same line direction since the large c component is the dominant contributor to the effective misorientation, whereas the effect of a component is rather negligible. Therefore, this effective penetration depth study uses ray tracing simulation images of deflected TSDs with photoelectric absorption applied to compare with all topographically observed Frank type dislocations. Analysis first reveals that the effective penetration depth varies with the line direction of a Frank type dislocation, and the effective penetration depth is significantly deeper compared to that of a BPD. Further, the effective penetration depth on ray tracing simulations with absorption applied matches well with experimentally measured depth. The study also evaluated the effectiveness of a simplified model based on an approximate expression for the effective misorientation of a dislocation modulated by photoelectric absorption. This was also found to yield satisfactory results and can be used as a universal method to determine the effective penetration depth for Frank type dislocations with c component of Burgers vector.

Alzheimer's disease: A role of biomarkers in early diagnosis and evidences from African ethnomedicinal knowledge.

Alzheimer's disease (AD) is a neurological ailment that primarily affects the elderly and necessitates an efficient treatment regimen backed up by extensive care. Despite advancement in the in vivo imaging techniques focussing on early diagnosis of reliable biomarkers using novel magnetic resonance imaging (MRI) and positron emission topography (PET) scans, AD remains largely unexplained and effective preventative and treatment strategies are still lacking. Consequently, research groups are constantly attempting to improve its early detection, using both invasive and non-invasive techniques with established core markers like Aβ and Tau (t-tau and p-tau) proteins. Unfortunately, African American and other black races are facing an increasing burden of closely associated risk factors, and only a few attempts have been made to find effective complementary and alternative therapies for AD cure and management. A greater epidemiology and natural product research are required to deal with the concurrent rise of dementia among quickly ageing African population, which so far have largely been ignored in addition to a disparity in the AD risk factors. We have tried to bring attention to the issue by reviewing up on this predisposition while generating a perspective on how race may affect AD risk and expression. This article also puts emphasis on finding new research leads from African phytodiversity while presenting several of the important species along with their biological agents found helpful in dementia related symptoms.