The paper analyses the influence and contribution of tone colour (timbre) of various musical instruments on successful notation of one-part and multi-part melodic dictation in solfeggio teaching during musical studies. An experimental pilot research tested the impact of tone colour of the most prevalent teaching tools - the piano, on the one hand, and the timbre of string instruments on the other - on the accuracy of identification of tone pitches, and therefore the accuracy of transcribing musical dictation. The research was based on the experiment with parallel groups on a sample of eighteen students of the second and fourth year of Faculty of Philology and Arts in Kragujevac. The students were divided into two groups - control and experimental group in each generation. The pilot research results prove that the use of timbre of string instruments provides greater accuracy of solving tests - writing multi-part (two-part and three-part) melodic dictation.