Tomato Tissues Research Articles

Chemical and mechanical coating of sulfur on baby corn biochar and their role in soil Pb availability, uptake, and growth of tomato under Pb contamination

In recent ages, industrial revolution and natural weathering processes have been increasing lead (Pb) contamination in agricultural soils, therefore, green remediation technologies are becoming attractive and cost-effective. In the current pot study, 1% and 2% (w/w) application rates of sulfur (S) alone and novel chemo-mechanically S-modified baby corn biochars (CSB and MSB) were applied in a Pb-contaminated (500 mg/kg) soil to evaluate tomato (Lycopersicon esculentum L.) growth, Pb uptake and its soil availability. The results from SEM-EDS and XRD patterns confirmed the S enrichment on the surface of baby-corn biochar. Further, Pb treatment alone imposed a significant reduction in biomass accumulation, photosynthetic pigments, antioxidative mechanism, root traits, and Pb-tolerance index because of increased soil Pb availability and its uptake, translocation and biological accumulation in various tissues of tomato. However, incorporation of lower rate of elemental S (1%) and higher rates of biochars, especially chemically S-modified biochar, CSB (2%) significantly improved dry biomass production, Pb-tolerance index, physiological attributes and antioxidative defense system of tomato plants. These results might be due to a prominent decrease in soil Pb availability by 37.5%, Pb concentration in shoot by 66.7% and root by 58.3%, soil to root transfer by 33.8%, and root to shoot transfer by 20.2% in tomato plants under 2% application rate of CSB, as compared with the Pb treatment without any amendment. Moreover, sulfur treatment induced a significant impact in reduction of soil pH (from 8.97-7.47) as compared to the biochar treatments under Pb-toxicity. The current findings provided an insight that 2% chemically S-modified biochar (CSB) has significant potential to improve the tomato growth by reducing Pb bioavailability in the Pb-contaminated soil, compared to the S alone and MSB amendments.

A group III WRKY transcription factor, SlWRKY52, positively regulates drought tolerance in tomato

Drought severely limits crop growth and development, reducing productivity. WRKY transcription factors play crucial roles in regulating diverse aspects of plant growth, development, and responses to stress. Despite this, the functions of tomato WRKYs (SlWRKYs) in response to drought stress remain incompletely understood. Thus, we isolated SlWRKY52, a member of the group III SlWRKYs from the tomato variety ‘Ailsa Craig’, and investigated its role in drought stress responses. SlWRKY52 is an unstable hydrophilic protein chiefly located in the nucleus, which promoter region features cis-acting elements engaged in drought stress response. It was expressed in all tomato tissues and its expression was rapidly induced by drought stress. SlWRKY52 overexpression lines conferred enhanced drought stress resistance in tomato: its involved in abscisic acid (ABA) synthesis, resulted in rapid, ABA-dependent stomatal closure, reduced leaf water loss, lessened photosynthetic damage, higher antioxidant enzyme activity, decreased oxidative damage parameters, increased osmoregulatory substance content, accelerated reactive oxygen species scavenging, and increased expression of regulatory interaction, ABA biosynthesis, and abiotic stress response-related genes. Conversely, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 nuclease mutant lines of SlWRKY52 reduced drought stress tolerance. In conclusion, our findings suggest that SlWRKY52 acts as a positive regulator of drought tolerance in tomato and has the potential to be used as a target gene for further molecular breeding.

Influence of Thermal Treatments, Extraction Methods, and Storage Conditions on Lycopene Content of Foods

Lycopene is one of the main carotenoids in our daily diet. Fruit variety, environmental conditions, and maturity stage are the factors affecting the content of lycopene. Various processing techniques and extraction methods may also affect the level of lycopene in different food products, consequently changing the biological role of lycopene. The biological role of lycopene is to defend the tissues of tomatoes (conjugate bonds) and attract predators (red colour). Moreover, storage conditions also impact the lycopene content of fruits, vegetables, and their products. Efficient and novel technological interventions are required for stabilizing lycopene content during postharvest procedures, such as refrigeration, heating, extraction, and transportation. Therefore, the study of different crucial factors concerning the change in lycopene content is required. The present review explores the lycopene content of different food commodities and the effect of postharvest operations and processing techniques on lycopene content. It also highlights the storage impact on the concentration of lycopene which may be useful for future studies.

Artificial trans-kingdom RNAi of FolRDR1 is a potential strategy to control tomato wilt disease.

Tomato is cultivated worldwide as a nutrient-rich vegetable crop. Tomato wilt disease caused by Fusarium oxysporum f.sp. Lycopersici (Fol) is one of the most serious fungal diseases posing threats to tomato production. Recently, the development of Spray-Induced Gene Silencing (SIGS) directs a novel plant disease management by generating an efficient and environmental friendly biocontrol agent. Here, we characterized that FolRDR1 (RNA-dependent RNA polymerase 1) mediated the pathogen invasion to the host plant tomato, and played as an essential regulator in pathogen development and pathogenicity. Our fluorescence tracing data further presented that effective uptakes of FolRDR1-dsRNAs were observed in both Fol and tomato tissues. Subsequently, exogenous application of FolRDR1-dsRNAs on pre-Fol-infected tomato leaves resulted in significant alleviation of tomato wilt disease symptoms. Particularly, FolRDR1-RNAi was highly specific without sequence off-target in related plants. Our results of pathogen gene-targeting RNAi have provided a new strategy for tomato wilt disease management by developing an environmentally-friendly biocontrol agent.

Comparative Analysis of the Expression Profiles of Pathogenesis-Related Genes in Tomato Systemically Infected with Tobacco Mosaic and Cucumber Mosaic Viruses

In this study, we used RT-qPCR to examine how PR genes were expressed in model tomato (Solanum lycopersicum L.) plants that had been infected with TMV or CMV. Under greenhouse conditions, the indirect ELISA data showed that both viruses were detected for the first time at 6 dpi. Then, the levels of accumulation increased very quickly, reaching a peak of 15 dpi. During the course of the study (1–15 dpi), the Delta CT, NormFinder, BestKeeper, and GeNorm software tools revealed that the β-actin gene was the most informative reference gene in the virally infected tomato tissues. For both the TMV- and CMV-infected tomato plants, the transcriptional expression levels of most tested genes changed between activation and repression, especially in the first 12 dpi. Compared to mock-inoculated plants, the expression levels of PR-1 were induced at all time intervals except at 8 dpi for CMV and at 6, 7, and 8 dpi for TMV infection. Conversely, the greater activation and accumulation of both viruses were associated with the greater up-regulation of PR-2 at 8 dpi, with relative expression levels of 7.28- and 5.84-fold for TMV and CMV, respectively. The up-regulated expression of PR-3, PR-4, and PR-7 was shown at 4 dpi. In contrast, the PR-5 gene was inhibited in TMV at 1 dpi until 9 dpi, and the induction of this gene at 10 dpi increased by 1.72-fold, but PR-5 was observed to up-regulate the expression of CMV at 1 dpi. This study provides the first valuable information on the comparative transcriptional levels of these tomato genes between TMV and CMV infections.

Localization of southern tomato virus (STV) in tomato tissues.

Southern tomato virus (STV) is a dsRNA virus, which belongs to the newly formed Amalgavirus genus of the Amalgaviridae family. Currently there is no report regarding the presence of STV in tomato tissues. In this study, we performed in situ hybridization to examine the distribution of STV in host tissues. STV was found in the leaves, stems, seeds, shoot apexes and root tips of tomato and localized in the cortex tissue, vascular tissues, pith, seed coat, endosperm, cotyledon (including inner cotyledons and outer cotyledons), hypocotyls and radicles of infected tomato tissues. In addition, STV was detected in the apical part of the stems and roots for the first time. This indicates that STV is a systemic infectious virus.

Exploring the Potential of Co-Application of Sewage Sludge, Chinese Medicinal Herbal Residues and Biochar in Minimizing Human Exposure to Antibiotics Contamination in Edible Crops

Agricultural practices such as wastewater irrigation and manure application may contaminate soils with antibiotics and, consequently, lead to human health risk. The co-application of three waste-derived materials, sewage sludge (SL), Chinese medicinal herbal residues (CMHR) and biochar (BC), as a soil amendment was proposed recently for minimizing the antibiotic amount in crop tissues. The fate of six antibiotics—amoxicillin, tetracycline, sulfamethazine, norfloxacin, erythromycin and chloramphenicol—were investigated in a greenhouse soil-plant system with a fruit crop species: tomato. The pots were mixed with 5%, 10% or 20% SL-BC and SL-CMHR-BC and irrigated with wastewater with 3 μg/L or 30 μg/L antibiotics. The pot containing 20% SL-CMHR-BC captured the lowest antibiotic concentration in soils and tomato tissues. Norfloxacin was the most abundant antibiotic in the fruits, followed by tetracycline. The pot containing 20% SL-CMHR-BC significantly lowered the bioconcentration factor of the fruit, while its effects on the translocation factor were more varied. Current and some previous data were used to assess the human health risk of consuming carrot, lettuce and tomato. The estimated daily intake suggested a negligible risk to human health in general compared with the acceptable daily intake, except for CAP. A concentration of 20% SL-CMHR-BC helps minimize the human exposure risk to antibiotics contamination in edible crops.

Complete Genome Sequence of Ralstonia solanacearum Strain Bs715, a Member of Biovar 4 and a Strong Pathogen of Bacterial Wilt on Solanum lycopersicum.

Ralstonia solanacearum is a notorious pathogen of bacterial wilt on Solanum lycopersicum. Most isolates from diseased tomato tissues are biovar 3, and their genomes are publicly available; however, information on biovar 4 strains is limited. Here, the complete genome sequence of R. solanacearum Bs715, a biovar 4 strain, is presented.

Screening and Characterization of Plant Growth Promoting Endophytic Microbes of Tomato (Solanum lycopersicum L.) combats Pythium aphanidermatum

About 45 bacteria, 9 fungi, 3 yeast, and 1 actinobacterium were isolated from plant tissues of tomato and screened for their plant growthpromoting traits and antagonistic activity against damping-off fungus Pythium aphanidermatum. The bacterial isolate, Bacillus pumilus TEB10 exhibited pathogen inhibition of about 55 and 79%, while yeast isolate Candida tropicalis TEY1 showed 30 and 68% in the dual plate and inverted plate assay, respectively. In addition, the Bacillus pumilus TB10 showed siderophore, HCN, cellulase, and phosphate solubilization activities. Similarly, yeast isolate Candida tropicalis TEY1 exhibited catalase, phosphate, and zinc solubilization activities. The sterol biosynthesis pathway end product ergosterol was profiled in secondary metabolites of P. aphanidermatum, suggesting the potentiality of this pathogenesis.

The DR5 and E8 reporters are suitable systems for studying the application of the <i>Ruby</i> reporter gene in tomato

<sec><title/> Tomato (<italic>Solanum lycopersicum</italic>) fruits can be found in a range of colors, including green, yellow, red, pink and purple. The various colors are determined by the accumulation of molecules such as chlorophyll, carotenoids (including lycopene) and flavonoids. Since the DR5 promoter is induced by auxin, we can infer that the auxin responses differ at different developmental stages in tomato. Fruit-specific promoters can effectively drive gene expression in fruits, and the E8 promoter is one of the most widely used fruit-specific promoters. In this study, DR5::<italic>Ruby</italic> transgenic tomato plants were used to further clarify the auxin content at different developmental stages in tomato. The results showed that the nonuniformity of <italic>Ruby</italic> activity was consistent with the natural distribution characteristics of native auxin, and the results were also consistent with the qRT‒PCR results. Furthermore, the accumulation of endogenous auxin at the immature green stage (IMG) was higher than that at the other stages. </sec><sec><title/> In addition, E8::<italic>Ruby</italic> was transformed into different tomato varieties (red, white and yellow) to generate new tomato varieties with fruit of different colors. The results showed that the E8::<italic>Ruby-</italic>transformed plants exhibited asymmetrical pink coloration in different tomato tissues of plants from different tomato cultivars. In conclusion, all the results showed that DR5 and E8 are suitable systems for studying the application of the <italic>Ruby</italic> reporter gene in tomato. Moreover, the study will provide insight for the improvement of tomato fruit color via biotechnology. </sec>

Enhanced ultrasonic spray ionization for direct mass spectrometry analysis of aqueous solution and complex samples using a single-orifice piezoelectric atomizer

An efficient and superior soft ionization approach for direct mass spectrometry analysis of a variety of samples such as aqueous solution, raw biological sample and proteins, was developed based on commercially available piezoelectric atomizers. A single conical orifice (5 μm in diameter) was created on the atomizer, which resulted in generation of uniform fine droplets and long-duration of MS signal. The two electrodes of piezoelectric atomizer were connected to the two sides of ceramic ring which was insulated from the metallic substrate. The unique design allowed an additional high voltage input towards the spray reagents, which facilitated direct analysis of more complex samples without sample pre-treatment, such as biological samples (tomato tissue). The ionization was driven by an extremely low electrical power (3.5 V rechargeable battery) yet providing an efficient and superior soft ionization. The method displayed a better thermal and pH stability than nano electrospray ionization (nanoESI) and electrospray ionization (ESI) on direct analysis of Vitamin B and protein aqueous solutions. Quantitative analysis of Vitamin B and Rhodamine B aqueous solutions was also investigated, showing a good linearity (R2 > 0.99). In addition, our results suggested that compared with ESI and nanoESI, the method not only could be used for direct analysis of intact protein, but also provide more information concerning the association between intact protein and the subunits.

Acetylation of a fungal effector that translocates host PR1 facilitates virulence.

Pathogens utilize a panoply of effectors to manipulate plant defense. However, despite their importance, relatively little is actually known about regulation of these virulence factors. Here, we show that the effector Fol-Secreted Virulence-related Protein1 (FolSvp1), secreted from fungal pathogen Fusarium oxysporum f. sp. lycopersici (Fol), directly binds and translocates the tomato pathogenesis-related protein1, SlPR1, from the apoplast outside the plasma membrane to the host nucleus via its nuclear localization signal. Relocation of SlPR1 abolishes generation of the defense signaling peptide, CAPE1, from its C-terminus, and as a consequence, facilitates pathogen invasion of plants. The action of FolSvp1 requires covalent modification by acetylation for full virulence in host tomato tissues. The modification is catalyzed by the Fol FolArd1 lysine acetyltransferase prior to secretion. Addition of an acetyl group to one residue, K167, prevents ubiquitination-dependent degradation of FolSvp1 in both Fol and plant cells with different mechanisms, allowing it to function normally in fungal invasion. Either inactivation of FolSvp1 or removal of the acetyl group on K167 leads to impaired pathogenicity of Fol. These findings indicate that acetylation can regulate the stability of effectors of fungal plant pathogens with impact on virulence.

Systematic Annotation Reveals CEP Function in Tomato Root Development and Abiotic Stress Response.

Tomato (Solanum lycopersicum) is one of the most important vegetable crops worldwide; however, environmental stressors severely restrict tomato growth and yield. Therefore, it is of great interest to discover novel regulators to improve tomato growth and environmental stress adaptions. Here, we applied a comprehensive bioinformatics approach to identify putative tomato C-TERMINALLY ENCODED PEPTIDE (CEP) genes and to explore their potential physiological function in tomato root development and abiotic stress responses. A total of 17 tomato CEP genes were identified and grouped into two subgroups based on the similarity of CEP motifs. The public RNA-Seq data revealed that tomato CEP genes displayed a diverse expression pattern in tomato tissues. Additionally, CEP genes expression was differentially regulated by nitrate or ammonium status in roots and shoots, respectively. The differences in expression levels of CEP genes induced by nitrogen indicate a potential involvement of CEPs in tomato nitrogen acquisition. The synthetic CEP peptides promoted tomato primary root growth, which requires nitric oxide (NO) and calcium signaling. Furthermore, we also revealed that CEP peptides improved tomato root resistance to salinity. Overall, our work will contribute to provide novel genetic breeding strategies for tomato cultivation under adverse environments.

ToBRFV Infects the Reproductive Tissues of Tomato Plants but Is Not Transmitted to the Progenies by Pollination.

Tomato brown rugose fruit virus (ToBRFV), a newly identified Tobamovirus, has recently emerged as a significant pathogen of tomato plants (Solanum lycopersicum). The virus can evade or overcome the known tobamovirus resistance in tomatoes, i.e., Tm-1, Tm-2, and its allele Tm-22. ToBRFV was identified for the first time only a few years ago, and its interactions with the tomato host are still not clear. We investigated ToBRFV’s presence in the reproductive tissues of tomato using fluorescent in situ hybridization (FISH) and RT-PCR. In infected plants, the virus was detected in the leaves, petals, ovary, stamen, style, stigma, and pollen grains but not inside the ovules. Fruits and seeds harvested from infected plants were contaminated with the virus. To test whether the virus is pollen transmitted, clean mother plants were hand pollinated with pollen from ToBRFV-infected plants and grown to fruit. None of the fruits and seeds harvested from the pollinated clean mother plants contained ToBRFV. Pollen germination assays revealed the germination arrest of ToBRFV-infected pollen. We concluded that ToBRFV might infect reproductive organs and pollen grains of tomato but that it is not pollen transmitted.

Study of influence of organochlorine pesticides and their destruction products on tomatoes in in vitro

Aim. Given the prospects of the method of detoxification of organochlorine contaminants in environment using microorganisms, the aim of the work was the investigation of the effect of microbial destruction products of pesticide hexachlorocyclohexane (HCCH) on morphogenetic and morphophysiological reactions of tomato in vitro. Methods. To test the effects of HCCH and its microbial degradation products obtained by strains of Bacillus megaterium, Pseudomonas putide 3, Stenotrophomonas maltophilia 6, these compounds were added to nutrient medium for tomato cultivation. As explants the segments of hypocotyls of L. esculentum seedlings were used. For plant regeneration, MS medium supplemented with 1 mg/l of zeatin and 1 mg/l IAA was used. Results. It was found that the products of HCCH destruction stimulate the shoot regeneration, but inhibit their rooting, whereas HCCH inhibits the shoot formation and provokes further morphophysiological changes in plants. Conclusions. A pronounced negative effect of pesticide HCCH on cells and tissues of tomato in vitro was revealed. It was found that the products of HCCH destruction do not affect the morphogenetic processes of isolated tomato cells and tissues and do not cause morphophysiological changes in obtained in vitro tomato plants.

GC–MS analysis, cytotoxicity, and molecular docking studies of bioactive alkaloids extracted from tomato leaves inoculated with endophytic fungus Beauveria sp. AUMC 15401

The term endophyte refers to a group of microbes that inhabit plant internal tissues such as bacteria and fungi. In this study, we isolated Beauveria sp. AUMC 15401 from the internal tissues of tomato leaves and extracted the alkaloidal contents from the leaves. Also, gas chromatography–mass spectrometry (GC–MS) analysis was used to identify the composition of the alkaloidal extract. The analysis showed that Beauveria sp. AUMC 15401 colonization stimulates plant secondary metabolism to produce novel alkaloids compared with the control tomato extract. Furthermore, the alkaloidal extract from the infected tomato leaves showed a high cytotoxic effect against A549 adenocarcinoma human alveolar basal epithelial cells. In addition, molecular docking studies were performed to investigate the potential binding of each alkaloid in the extract with epidermal growth factor receptor, vascular endothelial growth factor receptor 2, and SRC tyrosine kinases. These proteins are the main key factors in epithelial malignancies and their inhibition could reduce tumor growth. A pharmacokinetic study (absorption, distribution, metabolism, and excretion) was also conducted to determine the potential of the isolated alkaloids to be oral active candidates. Novelty impact statement Beauveria sp. AUMC 15401 colonization in tomatoes stimulates the production of the secondary metabolite of bioactive alkaloids that exhibited promising cytotoxic activity against A549 adenocarcinoma human alveolar basal epithelial cells. Multiple approaches have been used to assess the drug-like properties of the secreted alkaloids and verify their potential inhibitory action on the proteins that regulate cell proliferation and metastasis. This study emphasized the necessity of employing sustainable bioactive compounds from nature as a potential alternative to chemical-based therapies.

Biochar amendment reduces cadmium uptake by stimulating cadmium-resistant PGPR in tomato rhizosphere

Biochar amendment in the soil can exert a positive effect in reducing heavy metal toxicity in plants. However, it remains unclear the extent to which this effect is associated with the modulation of plant growth-promoting rhizobacteria (PGPR). Here, we initially conducted a pot experiment using tomato (Solanum lycopersicum L.) as a model plant grown in soil spiked with cadmium. First, we found biochar amendment to result in reduced cadmium uptake in tomato plants and trackable changes in the tomato rhizosphere microbiome. Then, a rhizosphere transplant experiment validated the importance of this microbiome modulation for cadmium-toxicity amelioration. Sequence-based analyses targeted the isolation of representative isolates of PGPR, including Bacillus and Flavisolibacter spp. that displayed in vitro cadmium tolerance and biosorption capabilities (in addition to abilities to solubilize phosphate and produce indole acetic acid). Last, we performed a soil inoculation experiment and confirmed the effectiveness of these isolates in reducing cadmium toxicity in tomato plants. Besides, we found the inoculation of these taxa as single inoculant and in combination to result in increased activities of specific antioxidant enzymes in tomato tissues. Taken together, this study revealed the ecological and physiological mechanisms by which biochar amendment indirectly alleviate cadmium toxicity in tomato plants, in this case, via the modulation and activity of specific PGPR populations. This study provides new insights into strategies able to promote beneficial PGPR in the rhizosphere with potential application to ameliorate heavy metal toxicity in plants.

Pulsed light of near-infrared and visible light wavelengths induces the accumulation of carotenoids in tomato fruits during post-treatment time.

Pulsed light (PL) is proposed as a novel strategy for the food industry to enhance the antioxidant potential of fruits and vegetables for industrial uses. The main aim of this work is to evaluate the impact of postharvest PL treatments of different spectral ranges on the carotenoid concentration as well as quality attributes of tomatoes during post-treatment time. Doses of wide-spectrum light (180-1100 nm), full-spectrum without ultraviolet (UV)-C wavelengths (305-1100 nm), and visible (VIS) + near-infrared light (NIR) (400-1100 nm) were compared. Total carotenoids, lycopene, and chlorophyll contents were spectrophotometrically assessed just after treatments and 1, 5, and 10 days post-treatment. PL treatments accelerated the accumulation of both total carotenoids and lycopene concentrations in tomato fruits. Nevertheless, the efficacy of PL depended on the applied spectral range. Tomato subjected to VIS + NIR treatment exhibited the greatest enhancement in total carotenoids (31 %) and lycopene (35 %) content at day 5 post-treatment and quality attributes were not affected. Conversely, UV-light exposure did not enhance carotenoid concentrations. These results evidenced that VIS + NIR treatments induced a faster accumulation of carotenoids without negatively affecting tomato quality attributes. PRACTICAL APPLICATION: The integration of visible and near-infrared (VIS + NIR) light filters in pulsed light (PL) processing allows enhancing the accumulation of bioactive compounds in tomato tissues in a sustainable way, which can be processed to obtain derived products (e.g., juices, purees) with health-promoting properties. PL technology is characterized by a lack of residual compounds and the absence of applying chemicals potentially harmful to humans. Industries can attract the attention of consumers through their application, which allows offering this added value.

New Insights into Bacillus-Primed Plant Responses to a Necrotrophic Pathogen Derived from the Tomato-Botrytis Pathosystem.

Induced systemic resistance (ISR) is one of the most studied mechanisms of plant–microbe interaction and is considered a very promising alternative for integrated pest management programs. In our study, we explored the plant defense response induced by Bacillus velezensis BBC047 in relation to its application before or after Botrytis cinerea infection of tomato plants. The inoculation of BBC047 did not considerably alter the gene expression of the tomato tissues, whereas infection with B. cinerea in BBC047-primed plants induced expression of LRR and NBS-LRR receptors, which are highly related to the ISR response. As expected, B. cinerea infection generated molecular patterns typical of a defense response to pathogen infection as the overexpression of pathogenesis-related proteins (PRs) in leaflets distant to the point of infection. The curative treatment (P + F + B) allowed us to gain insights into plant response to an inverted priming. In this treatment, B. cinerea caused the m tissue damage, extending nearly entirely across the entire infected leaves. Additionally, genes generally associated with early SAR response (<16 h) were overexpressed, and apparently, the beneficial strain was not perceived as such. Therefore, we infer that the plant defense to the curative treatment represents a higher degree of biological stress triggered by the incorporation of strain BBC047 as second arriving microorganism. We highlight the importance the phytosanitary status of plants prior to inoculation of beneficial microorganism for the biocontrol of pathogens.

Leaf Extracts from Resistant Wild Tomato Can Be Used to Control Late Blight (Phytophthora infestans) in the Cultivated Tomato.

Late blight disease, caused by Phytophthora infestans (Mont.) de Bary, is one of the most challenging diseases threatening tomato production and other Solanaceae crops. Resistance to late blight is found in certain wild species, but the mechanism behind the resistance is not fully understood. The aim of this study was to examine the metabolic profiles in the leaf tissue of late blight-resistant wild tomato and to investigate if leaf extracts from such genotypes could be used to control late blight in tomato production. We included three recognized late blight-resistant wild tomato accessions of Solanum habrochaites (LA1777, LA2855, and LA1352) and two recognized highly susceptible genotypes, S. lycopersicum (‘Super Strain B’) and S. pimpinellifolium (LA0375). The metabolic profiles were obtained in both inoculated and non-inoculated plants by analyzing leaf extracts using high-resolution gas chromatography-mass spectrometry (GC-MS) with three replicate analyses of each genotype. We focused on volatile organic compounds (VOCs) and identified 31 such compounds from the five genotypes with a retention time ranging from 6.6 to 22.8 min. The resistant genotype LA 1777 produced the highest number of VOCs (22 and 21 in the inoculated and control plants, respectively), whereas the susceptible genotype ‘Super Strain B’ produced the lowest number of VOCs (11 and 13 in the respective plants). Among the VOCs, 14 were detected only in the resistant genotypes, while two were detected only in the susceptible ones. In vitro trials, with the use of a detached leaflet assay and whole-plant approach, were conducted. We revealed promising insights regarding late blight management and showed that metabolic profiling may contribute to a better understanding of the mechanisms behind P. infestans resistance in tomato and its wild relatives.