Tocopherol Supplementation Research Articles

Effects of Maternal and Progeny Dietary Vitamin E on Growth Performance and Antioxidant Status of Progeny Chicks before and after Egg Storage.

Simple SummaryProlonged egg storage duration has been indicated to decrease the quality of hatchlings and the growth performance of offspring. Maternal nutrition plays a vital role in growth of chicks post-hatch. However, no work has been performed to evaluate whether or not maternal nutrition could improve the growth performance of offspring hatched from stored eggs. Here, we aimed to investigate the effects of maternal and progeny dietary vitamin E supplementation on the growth performance and antioxidant status of offspring before and after egg storage. Our results showed that maternal dietary vitamin E (VE) supplementation of 200 or 400 mg/kg could improve the growth performance and antioxidant status of offspring hatched from stored eggs, but not for that of offspring hatched from unstored eggs. These findings suggested that maternal dietary vitamin E was beneficial to improve the quality of long-term storage eggs.Two trials were conducted to investigate the effects of maternal and progeny dietary vitamin E (VE) supplementation on the growth performance and antioxidant status of offspring before and after egg storage. A total of 576 75-week-old Ross 308 breeder hens were assigned to three dietary VE treatments (100, 200, and 400 mg/kg) with 6 replicates of 32 hens for 12 weeks. Two trials were conducted with offspring hatched from eggs laid at weeks 9 and 12 of breeder feeding trial, respectively. Trial 1 was conducted by a 3 × 2 factorial arrangement of treatments with three levels of maternal dietary VE (100, 200, and 400 mg/kg) and two levels of progeny dietary VE (0 and 35 mg/kg). Trial 2 was conducted with three maternal dietary VE treatment (100, 200, and 400 mg/kg), and chicks were hatched from eggs stored for 14 d and received the same progeny diet with no addition of VE. Results showed that in trial 1, maternal (100, 200, and 400 mg/kg) and progeny (0 and 35 mg/kg) dietary VE supplementation did not affect the growth performance of offspring hatched from unstored eggs (p > 0.05). In trial 2, in the case of long-term egg storage, maternal dietary VE supplementation of 200 and 400 mg/kg increased the body weight (BW) of 21- and 42-d-old offspring and the body weight gain (BWG) of offspring from 1 to 21 d (p < 0.05), and decreased the feed conversion ratio (FCR) of offspring from 1 to 21 d (p < 0.05) compared to 100 mg/kg VE. As the maternal dietary VE levels increased, the liver and serum antioxidant status of offspring enhanced (p < 0.05). In conclusion, maternal dietary VE supplementation of 200 or 400 mg/kg could improve the growth performance and anti-oxidant status of offspring hatched from stored eggs, but not for that of offspring hatched from unstored eggs. The suitable VE level for the broiler breeder diet was 400 mg/kg in the case of long-term egg storage.

The impacts of egg storage time and maternal dietary vitamin E on the growth performance and antioxidant capacity of progeny chicks

Two trials were designed to investigate the impacts of egg storage time and maternal dietary vitamin E (VE) supplementation on the growth performance and antioxidant capacity of progeny chicks. In total 512 Ross 308 broiler breeder hens (71-wk-old) were assigned to 2 dietary VE treatments (6 and 100 mg/kg) for 14 wk. Progeny chicks used in trials 1 and 2 were originated from eggs laid at week 10 (stored 0 d) and week 8 (stored 14 d), and week 14 (stored 0 d) and week 12 (stored 14 d), respectively. The 4 groups in trial 1 consisted of 2 levels of maternal VE (6 and 100 mg/kg) and 2 egg storage time (0 and 14 d). The 8 groups in trial 2 consisted of 2 levels of maternal VE (6 and 100 mg/kg), 2 egg storage time (0 and 14 d) and progeny sex (male and female). In trial 1, egg storage decreased the body weight, the liver total superoxide dismutase and total antioxidant capacity of 21-day-old offspring (P < 0.05), and the body weight gain and feed intake from 8 to 21 d and 1 to 21 d (P < 0.05); and increased the serum and liver malonaldehyde (MDA) of 7-day-old offspring and the ratio of feed: gain (F/G) from 1 to 7 d (P < 0.05). Maternal VE (100 vs. 6 mg/kg) decreased the F/G from 1 to 7 d and increased the serum total superoxide dismutase of 21-day-old offspring (P < 0.05). In trial 2, egg storage decreased the body weight of 42-day-old offspring, and the body weight gain and feed intake from 22 to 42 d and 1 to 42 d (P < 0.05); and increased the serum and liver MDA of 21- and 42-day-old offspring (P < 0.05). Maternal VE (100 vs. 6 mg/kg) reduced the serum MDA of 7-day-old offspring (P < 0.05). Interactively, maternal VE (100 vs. 6 mg/kg) reduced the serum MDA of offspring originated from stored eggs (P < 0.05), but not for that of offspring originated from unstored eggs in the two trials. It can be concluded that egg storage (14 vs. 0 d) decreased the growth performance and antioxidant capacity of offspring, while maternal dietary VE (100 vs. 6 mg/kg) supplementation could partly alleviate the reduction of antioxidant capacity (except for growth performance) of offspring induced by egg storage for the early phase post-hatch.

Effect of Butylated Hydroxytoluene and Tocopherol Supplementation on In vitro Sperm Characters during Cryopreservation of Black Bengal Buck Semen

Background: Black Bengal goat is one of the important goat breeds of India. Cryopreservation of semen and artificial insemination are effective techniques for improving goat breeding programs. Various biochemical and functional changes that occur during freezing-thawing process results in poor post thaw sperm recovery. Supplementation of antioxidants to the extender has been reported to have positive effects on semen cryopreservation in various species. The present study was undertaken to assess the effect of antioxidants butylated hydroxyl toluene (BHT), α tocopherol on post thaw in vitro sperm characters, lipid peroxide level and superoxide dismutase (SOD) activity during cryo­preservation of Black Bengal buck semen. Methods: Semen was collected from bucks by artificial vagina, antioxidant BHT was added to the Tris egg yolk extender @ 0, 1 and 2 mM/ml in control group (BHTC), treatment group 1 (BHTT1) and treatment group 2 (BHTT2), respectively. Similarly, tocopherol was added @ 0, 1 and 2 mg/ml in control group (TFC), in treatment group 1 (TFT1) and treatment group 2 (TFT2), respectively. Each antioxidant was tested with 20 semen ejaculates. Semen samples were frozen in liquid nitrogen and post freeze-thaw in vitro sperm characters, malondialdehyde (MDA) concentration and SOD activity were measured. Result: Post thaw sperm motility, functional membrane integrity and viable count were significantly (P less than 0.05) higher in the BHT and α tocopherol supplemented groups than control groups. Significantly (P less than 0.05) higher acrosome intact cells were recovered in BHTT1, BHTT2 and TFT2 groups than the control and TFT1 groups. Further, BHT supplemented groups had significantly lower level of MDA than the control, while the supplementation of α tocopherol could not control the generation of MDA. Post thaw SOD activity was found significantly lower in the antioxidant treated groups than their respective controls. It is concluded that supplementation of BHT and α tocopherol were found to be more promising in conserving in vitro sperm characters from cryo-damages during freezing of Black Bengal bucks semen.

Supplementation of vitamin E and omega-3 fatty acids during the early posthatch period on intestinal morphology and gene expression differentiation in broilers

Early posthatch nutrition is important for gut health. Vitamin E (VE) and omega-3 (n-3) fatty acids can improve gut health through antioxidative and anti-inflammatory effects. The objective of this study was to identify the effects of VE, n-3 fatty acids, and combination of both during the starter phase (0–10 d) or grower phase (11–24 d) on intestinal morphology and expression of genes associated with gut health. A total of 210 Ross 708 broilers were randomly assigned into 7 treatments with 10 replicates of 3 birds each. The control group was fed a corn–soybean meal–basal diet during the entire study (0–58 d). Supplementation of VE (200 IU/kg), n-3 fatty acids (n-6/n-3 ratio of 3.2:1), and combination of both were fed during the starter phase (0–10 d) or grower phase (11–24 d). All of the broilers were harvested at 58 d of age. Villus height, crypt depth, villus width, distance between villi, and number of intraepithelial lymphocytes were obtained. Expression of 21 genes was measured using NanoString analysis. Expression of solute carrier family 15 member 1 (P = 0.01) associated with peptide transport and mucin 2 (P = 0.03) related with intestinal mucus barrier was increased in the broilers supplemented with n-3 fatty acids in the grower diet compared with the control. Expression of solute carrier family 7 member 1 associated with amino acid transport was decreased in the group supplemented with n-3 fatty acids during the starter phase compared with the group supplemented with n-3 fatty acids (P = 0.01) or VE and n-3 fatty acids during the grower phase (P = 0.03). These data suggest that VE and n-3 fatty acids supplemented during the grower phase have a positive effect on improving nutrient transport with n-3 fatty acids supplementation in the grower diet showing the most beneficial effect. These findings can be used in the development of nutritional management strategies to improve broiler growth performance and meat quality.

Effect of vitamin E and alpha lipoic acid on intestinal development associated with wooden breast myopathy in broilers

Intestinal development is closely associated with inflammatory wooden breast (WB) myopathy. Vitamin E (VE) and alpha lipoic acid (ALA) with antioxidant and anti-inflammatory effects were used independently and in combination to evaluate their effects on intestinal developmental changes in ileal morphology and expression of genes related with gut nutrient transport, structure, and inflammation in broilers during the first 3 wk posthatch. A total of 160 newly hatched Ross 708 broiler chicks were randomly assigned into a control and 3 dietary treatments with 10 replicates of 4 birds each. Supplementation of VE (160 mg/kg) and ALA (500 mg/kg) independently and in combination were fed during the first 3 wk. At 1, 2, and 3 wk of age, one chick from each pen was harvested. Plasma VE concentration and ileal morphology were determined. Gene expression was measured by real-time quantitative PCR. Broilers in VE and combination of ALA and VE group had higher plasma VE concentration than the control and ALA group at 1, 2, and 3 wk of age (P < 0.01). All dietary treatments increased ileal villus height at 1 wk of age (P < 0.01) and decreased intraepithelial lymphocytes at 3 wk of age compared to the control (P ≤ 0.05). Combination of VE and ALA increased collagen type IV alpha 1 chain expression (P ≤ 0.05) and improved basement membrane structure indicating increased gut basement membrane integrity at 2 and 3 wk of age compared to the control. Expression of lipopolysaccharide-induced tumor necrosis factor-alpha factor associated with inflammation was decreased in all dietary treatments at 3 wk of age compared to the control (P < 0.01). Ileal morphology and gene expression were closely correlated with breast muscle morphology and gene expression. These results suggest that VE and ALA especially when they were combined in the diet had positive effects on mitigating intestinal inflammation and improving nutrient transport beginning at 1 wk of age, which is likely critical in reducing the severity of WB.

The effect of vitamin E on sperm motility and viability in asthenoteratozoospermic men: In vitro study.

Induction of oxidative stress during the sperm preparation process for assisted reproductive techniques (ART) in men can weaken sperm parameters. Vitamin E (VE) is considered a factor in boosting male fertility. This experimental study (in vitro) aimed to assess the impact of VE supplementation on sperm quality and lipid peroxidation during sperm sampling at different times. For this mention, semen samples were collected from 50 asthenoteratozoospermic men. Samples were divided into control and test groups for 2, 4 and 6hr that the test group was incubated with VE (2mM). In two groups, total motility, progressive motility and viability based on the WHO 2010 criteria were assessed. Moreover, malondialdehyde (MDA) levels were evaluated in each group. In the control group, total and progressive motility and sperm viability were decreased significantly after 2hr; however, MDA levels were increased significantly after 6hr. Also, in the test group, sperm parameters were increased significantly after 2hr, and MDA levels were decreased significantly after 6hr compared to the control group. In outcome, in vitro VE supplementation may protect spermatozoa from the adverse effect of oxidative stress during sperm preparation via preservation antioxidant processes in normal condition.

Effect of early posthatch supplementation of vitamin E and omega-3 fatty acids on the severity of wooden breast, breast muscle morphological structure, and gene expression in the broiler breast muscle

Wooden breast (WB) has arisen primarily in the breast muscle of commercial broilers. It is characterized by palpation of a rigid pectoralis major (p. major) muscle and is under severe oxidative stress and inflammation. Previous studies have shown that vitamin E (VE) has antioxidant properties and omega-3 (n-3) fatty acids have an anti-inflammatory effect. The objectives of this study were to identify the effects of VE and n-3 fatty acids on the severity of WB, morphological structure of the p. major muscle, expression of genes likely associated with WB and to determine the most beneficial supplementation period. A total of 210 Ross 708 broilers were randomly assigned into 7 treatments with 10 replicates of 3 birds each. The control group received a corn–soybean meal basal diet during the entire study (0–58 d). Supplementation of VE (200 IU/kg), n-3 fatty acids (n-6/n-3 ratio of 3.2:1), or combination of both were fed during the starter phase (0–10 d) or grower phase (11–24 d). All broilers were harvested at 58 d of age. Morphological assessment of the p. major muscle included myofiber width, perimysial and endomysial connective tissue space, overall morphological structure, and scoring of WB microscopically. Gene expression was measured using nanostring analysis. Genes associated with muscle development and growth factors, inflammation, extracellular matrix, and glucose metabolism were differentially expressed in the p. major muscle of the broilers supplemented with VE in the grower diet. Greater than 2 times more giant myofibers (≥70 μm) were found in the group supplemented with VE and n-3 fatty acids in the starter diet compared with the group fed VE in the grower diet (P = 0.02). Microscopic evaluation showed that VE supplementation in the grower diet had a 16.19% increase in muscle with no WB compared with the control group (P = 0.05). These data suggest that supplementation of VE during the grower phase may reduce the severity of WB in broilers.

Effects Of 16 Weeks Hiit And Vitamin E On The Ros-nfkb-bnip3 Pathway In Aging Rat Muscle

The effect of high intensity interval exercise (HIIT) combined with Vitamin E(VE) in the intervention of aging degeneration of skeletal muscle is still unclear. PURPOSE: In this study, the effects of 16-week HIIT intervention and VE supplementation on the mitochondrial autophagy related Ros-NFκB-Bnip3 pathway and the number of mitochondria in aged rat skeletal muscle were observed. To provide theoretical basis for delaying sarcopenia by means of exercise and nutrition. METHODS: 60 male Wistar rats aged 8 months were randomly divided into the control group (C, n=20), the HIIT intervention group (H, n=20) and the HIIT group supplemented with VE (EH, n=20) according to the random number table. H and EH adjusted the exercise intensity with the results of the VO2max test every two weeks. The EH was given VE gavage according to 50mg/kg body weight everyday at 1 hour before each training. Rats were taken from each group randomly at the basic state, week 8 and 16 for sampling. During sampling, the soleus muscle of rats was removed. The mitochondria were photographed using a transmission electron microscope, the fluorescence intensity of ROS was measured by Multifunctional enzyme marker, The NF-κB, beclin-1 and Bnip3 were measured by Western blot. The data were analyzed by multivariate ANOVA. RESULTS: At 8 weeks, the number of mitochondrial in H and EH were increased (P=0.033, P=0.001) and higher than that in C (P=0.046, P=0.003). At 16 weeks, H was higher than C (P=0.004), EH (P=0.01) and baseline (P=0.046). ROS in C and EH increased at 8 weeks (P=0.024, P=0.001). C and EH were higher than H (P=0.018, P=0.002). At 16 weeks, H and EH were higher than baseline values (P=0.027, P=0.010). Although the content of NF-κB changed in each group, no significant difference occurred. At week 16, beclin-1 expression of soleus muscle in H increased (P=0.036).EH was lower than H and C (P=0.004, P=0.002). The expression of Bnip3 in soleus muscle in C increased at 8 weeks and 16 weeks (P=0.030, P=0.001). H increased at 8 and 16 weeks (P=0.001, P=0.001), and was higher in H than in EH (P=0.006, P=0.020). CONCLUSIONS: The 16 week HIIT intervention and the combined effect of HIIT and VE to alleviate the reduction of mitochondrial number caused by aging may be due to the delayed protein expression of the Ros-NFκB-Bnip3 pathway in the soleus muscle of rats.

Vitamin E improves antioxidant status but not lipid metabolism in laying hens fed a aged corn-containing diet.

ObjectiveThe objective of this study was to determine whether a dietary vitamin E (VE) supplement could alleviate any detrimental effects of aged corn on lipid metabolism and antioxidant status in laying hens.MethodsThe experiment consisted of a 2×3 factorial design with two corn types (normal corn and aged corn (stored for 4 yr) and three concentrations of VE (0, 20, and 100 IU/kg). A total of 216 Lohmann laying hens (50 wk of age) were randomly allocated into six treatment diets for 12 wk. Each treatment had 6 replicates of 6 hens per replicate.ResultsThe results show that aged corn significantly decreased the content of low-density lipoprotein cholesterol (p<0.05), and reduced chemokine-like receptor 1 (CMKLR1) mRNA expression (p<0.05) in the liver compared to controls. Diet with VE did not alter the content of crude fat and cholesterol (p>0.05), or acetyl-CoA carboxylase, lipoprotein lipase, fatty acid synthase or CMKLR1 mRNA expression (p>0.05) in the liver among treatment groups. Aged corn significantly increased the content of malondialdehyde (MDA) (p<0.05) and decreased superoxide dismutase (SOD) activity (p<0.05) in the liver. The VE increased the content of MDA (p<0.05) but decreased glutathione peroxidase (GSH-Px) activity in serum (p<0.01) and in the ovaries (p<0.05). Adding VE at 20 and 100 IU/kg significantly increased GSH-Px activity (p<0.05) in liver and in serum (p<0.01), 100 IU/kg VE significantly increased SOD activity (p<0.05) in serum. Aged corn had no significant effects on GSH-Px mRNA or SOD mRNA expression (p<0.01) in the liver and ovaries. Addition of 100 IU/kg VE could significantly increase SOD mRNA expression (p<0.01) in the liver and ovary.ConclusionAged corn affected lipid metabolism and decreased the antioxidant function of laying hens. Dietary VE supplementation was unable to counteract the negative effects of aged corn on lipid metabolism. However, addition of 100 IU/kg VE prevented aged corn-induced lipid peroxidation in the organs of laying hens.

Effect of vitamin E and omega-3 fatty acids early posthatch supplementation on reducing the severity of wooden breast myopathy in broilers

The wooden breast (WB) myopathy is identified by the palpation of a rigid pectoralis major (P. major) muscle and is characterized as a fibrotic, necrotic P. major muscle disorder in broilers resulting in reduced breast meat quality. Breast muscle affected with WB is under severe oxidative stress and inflammation. The objectives were to identify the effects of dietary vitamin E (VE) and omega-3 (n-3) fatty acids independently or in combination when fed during the starter phase (0–10 D) or grower phase (11–24 D) on growth performance, meat yield, meat quality, and severity of WB myopathy and to determine the most beneficial dietary supplementation period. A total of 210 Ross 708 broiler chicks were randomly assigned into 7 experimental groups with 10 replicates of 3 birds each. The control group was fed with corn–soybean meal basal diet with VE (10 IU/kg) and n-3 fatty acids (n-6/n-3 ratio of 30:1) at a standard level during the entire study (0–58 D). Supplementation of VE (200 IU/kg), n-3 fatty acids (n-6/n-3 ratio of 3:1), or combination of both was performed during the starter phase or grower phase. Growth performance, meat yield, meat quality, and WB scores were obtained. There was no significant difference in final body weight and meat yield when VE was increased (P > 0.05). In contrast, n-3 fatty acids supplementation in starter diets significantly decreased final body weight, hot carcass weight, and chilled carcass weight of broilers (P ≤ 0.05). The P. major muscle from broilers supplemented with VE in starter diets had lower shear force than in grower diets (P ≤ 0.05). Supplemental VE reduced the severity of WB and in starter diets showed a more beneficial effect than those fed VE in the grower diets. These data are suggestive that additional supplementation of dietary VE may reduce the severity of WB and promote breast meat quality without adversely affecting growth performance and meat yield.

Effects of dietary vitamin E supplementation on the reproductive performance of yearling female mink (Neovison vison) fed wet fish-based feed

This study was conducted to investigate the effects of dietary vitamin E (VE) supplementation on the reproductive performance of female mink, preweaning growth performance of their kits, and antioxidative status and immune functions of dams and kits. Yearling mink dams (n = 180) were randomly assigned to five treatment groups (n = 36). The dietary treatments included a basal diet supplemented with VE at 0 (control), 80, 160, 320, or 640 mg/kg DM. Compared with the control, the addition of 160–320 mg/kg VE decreased (P = 0.0362) the percentage of pre-weaning mortality of mink kits. At weaning, the average body weight was greater (P = 0.0408) in kits fed 320 mg/kg supplemental VE. In mink dams, the addition of 80–320 mg/kg VE increased (P = 0.0125) serum SOD. Supplementation of 320 or 640 mg/kg VE decreased (P = 0.0260) serum reactive oxygen species (ROS). Furthermore, feeding a large dose of VE (640 mg/kg diet) resulted in increased (P = 0.0245) serum α-tocopherol. In mink kits, the addition of 320 or 640 mg/kg VE increased serum α-tocopherol (P = 0.0207) and IgG (P = 0.0464). Supplementation of 640 mg/kg VE decreased (P = 0.0471) serum ROS. The present results indicate that VE supplementation improved the antioxidative status of mink dams and enhanced the immune functions, decreased pre-weaning mortality and enhanced weaning weight of their kits. Taken together, the effective VE supplementation was 320 mg/kg of diet for yearling female mink.

Effects of dietary vitamin E on the growth performance, antioxidative status, and some immunological blood parameters in growing mink (Mustela vison) fed dry feed

Ninety standard dark male minks (8 wk of age) were used to investigate the effects of vitamin E (VE) supplementation on growth performance, antioxidative status, and some immunological blood parameters. The dietary treatments included a basal diet (containing 20.86 mg kg−1VE) supplemented with 0 (control), 50, 100, 200, 400, or 800 mg kg−1VE. The results showed that VE supplementation of 200–400 mg kg−1increased (P &lt; 0.05) the body weight, average daily feed intake, average daily gain, and gain to feed ratio of the mink from days 1 to 30. At days 30 and 60, the minks fed diets supplemented with 400 mg kg−1VE had higher (P &lt; 0.05) concentrations of serum superoxide dismutase, glutathione peroxidase, and catalase than either the control or the VE50 groups but had activity levels similar to those of the VE200 and VE800 groups. Feeding a high dose of VE (400–800 mg kg−1diet) resulted in a significant increase in the concentrations of α-tocopherol and a reduction in the reactive oxygen species content in the serum. Vitamin E supplementation of 200–400 mg kg−1increased (P &lt; 0.05) the concentrations of immunoglobulin G, interleukin-2, and soluble CD4/soluble CD8and decreased (P &lt; 0.05) the content of soluble CD8in the serum. Overall, the suitable level of VE supplementation was found to be 200–400 mg kg−1diet for growing mink.

Dietary supplementation of vitamin E and citric acid could significantly promote the relative expression of PPARα and aconitase genes, concentration of polyunsaturated fatty acids, antioxidant enzyme activities, and growth of juvenile cobia

The experiment was to study the effect of different dietary vitamin E (VE) and/or citric acid (CA) supplementation on the peroxisome proliferator-activated receptor α (PPARα) and aconitase (ACO) gene expression, fatty acids, antioxidant enzyme activities, and growth of juvenile cobia (Rachycentron canadum). Seven groups of cobia juveniles in triplicate were cultured in tanks using filtered and aerated seawater. The juveniles were fed twice a day using 7 specific diets: control diet (D0) without supplementation; Diets 1 to 6 (D1 to D6) were supplemented with CA 0, 12, 12, 6, 3, 1.5 g and VE 100, 0, 100, 75, 50, 25 IU per kg of dried feed, respectively. The juveniles were fed for 12 weeks and their liver, muscle, and serum were sampled randomly for analysis in weeks 0 and 12. The results showed that the supplementation of VE plus CA could significantly promote the relative mRNA expression of ACO and PPARα genes, concentration of polyunsaturated fatty acids (PUFAs), activity of antioxidant enzymes, and growth of juvenile cobia. The effect of both dietary VE plus CA supplementation on the above indexes was significantly greater (P < 0.05) than a single VE (D1) or CA (D2) supplementation. The optimum diet was D5, with a VE of 50 IU and a CA of 3 g supplementation per kg of dried feed in the tested diets.

VitaminE-blended versus conventional polyethylene liners in prostheses : Prospective, randomized trial with 3-year follow-up.

Despite continuous technical improvements, polyethylene wear debris induced periprosthetic osteolysis remains the main cause for failure of hip arthroplasty. Progressive oxidation of polyethylene was identified as another risk factor for material failure. To overcome this problem, antioxidants such as vitamin E (alpha-tocopherol) were supplemented by diffusion into the latest generation of polyethylene liners. The purpose of the present study was to investigate the clinical outcome of patients treated with vitaminE blended highly cross-linked ultra-high molecular weight polyethylene liners (UHMWPE-XE) in comparison with conventional UHMWPE‑X liners by evaluating patient-reported outcome measures (PROM's) at 3‑year follow-up. Atotal of 143patients were recruited into this prospective, randomized trial in our academic center. Three years after implantation, 101 patients were examined in the outpatient clinic for follow-up. Of these, 51 (50.5%) received UHMWPE-XE and 50 (49.5%) UHMWPE‑X liners. Clinical outcome was evaluated using Harris-Hip-Score (HHS) UCLA-Score and Hip Disability and Osteoarthritis Outcome Score (HOOS). There was asignificant improvement in all PROM's at one- and three-year follow-up compared to the status before implantation. PROM's did not differ significantly between the first and third year follow-up. Both liner groups showed an equal clinical outcome. The present study demonstrates that the supplementation of vitaminE to polyethylene liners is reliable and safe without showing higher complication rates compared with conventional polyethylene liners. The shortterm clinical outcome of vitamin E-blended (UHMWPE‑XE) is equivalent to those of conventional highly cross-linked polyethylene liners.

Protective Effect of Vitamin E on laying performance, antioxidant capacity, and immunity in laying hens challenged with Salmonella Enteritidis

Vitamin E (VE) has proven to function as potent lipid-soluble antioxidant, a signaling molecule, and a regulator of the immune system. The objective of the study was to assess the protective effect of VE on laying performance, antioxidant capacity, and immunity in laying hens exposed to Salmonella Enteritidis (SE). A total of 80 32-week-old salmonella-free double negative Hy-Line brown laying hens were randomly assigned to 4 treatments with 20 replicates each (1 bird per replicate) according to a 2 × 2 factorial design with 2 VE supplementation levels [0IU/kg (VE0) vs. 30IU/kg (VE30)], and 2 challenge treatments [SE vs. physiological saline solution (PS)]. During the last 3 D of week 43 of age, birds were orally challenged with 1.0mL suspension of 109 cfu/mL S. Enteritidis daily, whereas the birds of negative treatments (VE0) received the same volume of PS. The egg mass of VE0 treatment decreased (P < 0.05) in contrast to VE treatment after challenge. The serum concentrations of interleukins (IL-1β and IL-6) and malondialdehyde (MDA) levels of SE treatments increased (P < 0.05) at week 44 and week 46, respectively. In both VE30 treatments, the decrease (P < 0.05) in birds' mortality was associated with higher IgA, IgG, IgM concentrations at week 44, and higher IgA, IgM concentrations at week 46. There is an interaction (P < 0.05) between SE challenge and VE levels with regard to feed conversion, daily egg mass, and serum MDA, IgA, and IgM levels. It can be concluded that supplemental VE (30IU/kg) in diets for laying hens may alleviate oxidative and immune stress due to SE challenge.

Effect of vitamin E sources and inclusion levels in diets on growth performance, meat quality, alpha-tocopherol retention, and intestinal inflammatory cytokine expression in broiler chickens

Water-miscible vitamin E (WVE) has been known to be more bioavailable than conventional lipid-soluble vitamin E (LVE) in human foods because of its greater solubility in the gastrointestinal tract. However, no data regarding the comparison of the efficacy of WVE to LVE were available in poultry diets. The objective of the current experiment was to investigate the effect of vitamin E (VE) sources and inclusion levels in diets on growth performance, meat quality, alpha-tocopherol retention, and intestinal inflammatory cytokine expression in broiler chickens. A total of 420 6-d-old broiler chicks were allotted to 1 of 7 dietary treatments with 6 replicates in a completely randomized design. The corn-soybean meal-based basal diet was formulated without inclusion of supplemental VE. Additional 6 diets were prepared by supplementing the basal diet with either WVE or LVE at the inclusion levels of 33, 65, or 100IU/kg in diets. Diets were fed to birds for 26 d. Results indicated that different VE sources and inclusion levels from 33 to 100IU/kg in diets had no effects on growth performance in broilers. The VE sources did not affect lipid oxidation in both breast and thigh meat. However, increasing inclusion levels of VE decreased (linear and quadratic, P < 0.05) lipid oxidation in breast meat. Regardless of VE sources, increasing inclusion levels of VE increased (linear, P < 0.01) alpha-tocopherol concentrations in breast meat. Dietary WVE showed a greater reduction in the expression of both pro- and anti-inflammatory cytokine genes in the jejunum than dietary LVE. Increasing inclusion levels of both VE sources decreased (linear and quadratic, P < 0.05) the expression of both pro- and anti-inflammatory cytokine genes in the jejunum. The efficacy of dietary WVE for growth performance, meat quality, lipid oxidation, and alpha-tocopherol retention in broilers chickens is similar to that of dietary LVE. However, it is likely that dietary WVE is more effective in decreasing intestinal inflammatory responses than dietary LVE in broilers.

Vitamin E supplementation strategies during feedlot receiving: effects on beef steer performance, antibody response to vaccination, and antioxidant defense1.

This study utilized 204 Angus-based beef steers (249 ± 23 kg SD) from a single ranch with initial serum α-tocopherol concentrations of 3.9 ± 1.0 mg/L to determine the effect of varying doses of vitamin E (VE) on feedlot performance, antibody response to vaccination, and antioxidant defense. Seven days after arrival, steers were blocked by body weight and weaning protocol (preweaned, unweaned heavy, and unweaned light) and randomly assigned to pens within blocks (12 pens per block). Preweaned steers had been weaned for approximately 35 d prior to arrival, and unweaned steers were weaned when leaving the origin ranch. Pens within block were randomly assigned to supplemental VE (ROVIMIX E-50 Adsorbate, DSM Nutritional Products, Heerlen, The Netherlands) treatments (n = 9 pens per treatment): no supplemental VE (CON), 25 IU/kg dry matter (DM; LOW), 500 IU per steer daily (MED), or 1,000 IU per steer daily (HIGH). Back-calculated supplemental VE intake was 0, 151 (24.8 IU/kg DM), 484, and 995 IU/d for CON, LOW, MED, and HIGH, respectively. On day 6, all steers received a booster vaccine against bovine viral diarrhea virus (BVDV; Bovi-Shield Gold, One Shot, Zoetis, Parsippany, NJ). Steers were weighed on day -1, 0, 14, 26, and 27. One steer per pen representative of the average body weight of the pen was chosen as a sampling animal for blood (day -1, 6, 14, 26, and 28) and liver (day -3 and 24). Data were analyzed as a randomized complete block design using Proc Mixed of SAS with pen as the experimental unit and the fixed effects of treatment and block. Linear, quadratic, and cubic contrast statements were constructed using Proc IML; morbidity data were analyzed using Proc Glimmix. Day 24 liver and day 26 serum α-tocopherol concentrations were linearly increased by supplemental VE (P < 0.01). Supplemental VE did not affect DM intake, average daily gain, or gain:feed from day 0 to 27 (P ≥ 0.37), or the percentage of steers treated for respiratory disease (P ≥ 0.44). Day 24 liver glutathione concentrations decreased linearly due to supplemental VE (P ≤ 0.02). Total- and Mn-superoxide dismutase activities were quadratically affected by supplemental VE (P ≤ 0.07), with MED steers exhibiting the greatest activity. Over time, BVDV type 1 and 2 antibody titers numerically decreased, whereas the decrease in BVDV type 1 titers was lesser for HIGH steers (linear P = 0.04). Increasing doses of VE improved VE status but did not affect overall receiving period performance in steers with minimal to adequate VE status upon arrival.

Effect of vitamin E in ovo feeding to broiler embryos on hatchability, chick quality, oxidative state, and performance.

The objective of the present study was to ascertain the effect of in ovo feeding of vitamin E (VE) on the incubation results, quality, and oxidative state of newborn chicks and on the initial performance results. The design consisted of randomized blocks with treatments at different levels of VE (0.0, 27.5, 38.5, 49.5, and 60.4 IU). On 17.5 d of embryonic development, 780 eggs underwent in ovo injection using a manual needle. VE supplementation of 60.4 IU provided the highest hatching rate (P < 0.05) and shortest hatch window (P < 0.05). Better results regarding chick physical quality were observed in groups supplemented with VE (body weight, length, newborn chick quality score) and higher chick weight/egg weight ratios (P < 0.05). VE inoculation did not have any effect on the chicks' immunological system (P > 0.05). Greater development of the small intestine (intestine weight/yolk free chick weight and higher villi in duodenum) and better feed conversion over all periods studied (1 to 7, 1 to 14, and 1 to 21 d) were observed among chicks that received in ovo VE supplementation (P < 0.05). The total protein concentrations in the liver and striated breast skeletal muscle tissue were highest in chicks that received 60.4 IU of VE (P < 0.05). The highest catalase activity was observed in the livers of newborn chicks supplemented with 60.4 IU of VE (P < 0.05). It was concluded that in ovo VE supplementation improved the chicks' oxidative state, which led to improvements in incubation results, chick quality and performance results.

PSV-23 Effects of different fat sources and vitamin E levels on tissue tocopherol concentration, antioxidant status, and fatty acid composition in the backfat of pigs grown to 150 kg

Abstract The study objective was to assess the contribution of fat source and vitamin E (VE) supplementation on tissue tocopherol concentration, antioxidant status, and fatty acid composition in the backfat of pigs at heavy slaughter weight (149.38 ±1.56 kg). A total of 64 individually-fed pigs (32 barrows, 32 gilts; 28.41 ±0.83 kg) were randomly assigned to 8 dietary treatments in a 4 × 2 factorial arrangement. Fat treatments included corn-starch (CS), tallow (TW), corn oil (CO) and coconut oil (CN). VE supplementation levels were 11 and 200 IU/kg. Loin muscle, backfat, and liver samples were collected at slaughter. Data analysis were performed by ANOVA using GLM in SAS. No interactions between dietary fat source and VE supplementation were observed. Increasing dietary VE level from 11 to 200 IU/kg increased (P &lt; 0.001) the tocopherol concentration in both liver (4.73 vs. 21.06 ppm wet liver) and loin muscle (1.25 vs. 2.67 ppm wet muscle). Pigs from the CO group had higher liver SOD activity (P &lt; 0.05) than the other treatments (CS 22.51, TW 22.67, CO 24.40, and CN 20.92 U/mg protein). Pigs from the CN group had the highest (P &lt; 0.05) total saturated fatty acid content (CS 44.33, TW 40.33, CO 36.29, and CN 51.70%), but the lowest (P &lt; 0.05) total polyunsaturated fatty acid content (CS 9.26, TW 9.80, CO 25.41, and CN 8.75%) and iodine value (CS 56.61, TW 60.66, CO 77.83, and CN 49.90) in the backfat. Pigs from the TW group had the highest (P &lt; 0.05) total monounsaturated fatty acid content (CS 46.40, TW 49.87, CO 38.30, and CN 39.56%) in the backfat. Under conditions of this study, a higher level of VE supplementation increased liver and muscle tocopherol concentrations whereas dietary fat sources altered liver antioxidant activity and fatty acid composition in the backfat.

Effect of different fat sources and vitamin E status/levels on growth performance, antioxidant status, carcass characteristics, fatty acid profile, and meat quality of pigs grown to heavy slaughter weight at 150 kg

Abstract Two experiments were used to evaluate the potential interaction of fat source and vitamin E (VE) in heavy slaughter weight pigs. In Experiment 1, a total of 64 individually-fed pigs (28.41 ± 0.83 kg) were randomly assigned to 8 dietary treatments in a 4×2 factorial arrangement. Fat treatments included cornstarch (CS), tallow (TW), corn-oil (CO), and coconut-oil (CN). VE treatments were dietary α-tocopheryl-acetate (ATA) at 11 and 200 ppm. In Experiment 2, a total of 72 individually fed pigs (28.55 ± 1.16 kg) were randomly assigned to 12 dietary treatments in a 2×6 factorial arrangement. Fat treatments were TW and CO. VE treatments included four levels of ATA (11, 40, 100, and 200 ppm) and two levels of mixed tocopherols (primarily γ-tocopherol; 40 and 100 ppm). VE deposition, growth performance, and meat quality were measured in both experiments. In both experiments, interaction between fat sources and VE were detected (P &lt; 0.01) on plasma VE concentration, which increased (P &lt; 0.01) with time and with increasing dietary VE, but increased faster (P &lt; 0.05) in pigs fed with CN and TW compared to pigs fed CS and CO. Compared to CO, more saturated dietary fat sources (CN and TW) led to firmer belly (P &lt; 0.01), which had more (P &lt; 0.01) SFA and MUFA while less (P &lt; 0.01) PUFA. In Experiment 1, increasing dietary ATA decreased (P &lt; 0.05) FCR in Phase 4 and Phase 5. In Experiment 2, increasing dietary ATA increased overall ADG (linear, P = 0.02), with an interaction (P &lt; 0.05) with fat sources on cumulative ADG during Phase1-4, wherein pigs fed CO, but not TW, had increased ADG with increasing dietary ATA. Increasing dietary ATA increased (quadratic, P &lt; 0.05) liver SOD activity, and decreased (quadratic, P &lt; 0.05) liver MDA content. The oxidative stability of loin was improved (P &lt; 0.01) when dietary ATA increased over 40 ppm. In summary, both dietary fat source and VE supplementation affected the response measures.