Effects Of 16 Weeks Hiit And Vitamin E On The Ros-nfkb-bnip3 Pathway In Aging Rat Muscle

Abstract

The effect of high intensity interval exercise (HIIT) combined with Vitamin E(VE) in the intervention of aging degeneration of skeletal muscle is still unclear. PURPOSE: In this study, the effects of 16-week HIIT intervention and VE supplementation on the mitochondrial autophagy related Ros-NFκB-Bnip3 pathway and the number of mitochondria in aged rat skeletal muscle were observed. To provide theoretical basis for delaying sarcopenia by means of exercise and nutrition. METHODS: 60 male Wistar rats aged 8 months were randomly divided into the control group (C, n=20), the HIIT intervention group (H, n=20) and the HIIT group supplemented with VE (EH, n=20) according to the random number table. H and EH adjusted the exercise intensity with the results of the VO2max test every two weeks. The EH was given VE gavage according to 50mg/kg body weight everyday at 1 hour before each training. Rats were taken from each group randomly at the basic state, week 8 and 16 for sampling. During sampling, the soleus muscle of rats was removed. The mitochondria were photographed using a transmission electron microscope, the fluorescence intensity of ROS was measured by Multifunctional enzyme marker, The NF-κB, beclin-1 and Bnip3 were measured by Western blot. The data were analyzed by multivariate ANOVA. RESULTS: At 8 weeks, the number of mitochondrial in H and EH were increased (P=0.033, P=0.001) and higher than that in C (P=0.046, P=0.003). At 16 weeks, H was higher than C (P=0.004), EH (P=0.01) and baseline (P=0.046). ROS in C and EH increased at 8 weeks (P=0.024, P=0.001). C and EH were higher than H (P=0.018, P=0.002). At 16 weeks, H and EH were higher than baseline values (P=0.027, P=0.010). Although the content of NF-κB changed in each group, no significant difference occurred. At week 16, beclin-1 expression of soleus muscle in H increased (P=0.036).EH was lower than H and C (P=0.004, P=0.002). The expression of Bnip3 in soleus muscle in C increased at 8 weeks and 16 weeks (P=0.030, P=0.001). H increased at 8 and 16 weeks (P=0.001, P=0.001), and was higher in H than in EH (P=0.006, P=0.020). CONCLUSIONS: The 16 week HIIT intervention and the combined effect of HIIT and VE to alleviate the reduction of mitochondrial number caused by aging may be due to the delayed protein expression of the Ros-NFκB-Bnip3 pathway in the soleus muscle of rats.