Tobacco Research Articles

A polar viscosity-sensitive fluorescent probe with large Stokes shifts for simultaneous imaging of lipid droplets and lysosomes in tobacco leaf vein cells and biological systems.

Lipid droplets (LDs) and lysosomes were dynamic organelles present in most eukaryotic cells that were interconnected and worked closely together to ensure the smooth physiological activities of organisms. The interaction between lipid droplets and lysosomes was thought to play a role in the development of certain diseases. In this paper we designed and synthesised a lipid droplet lysosomal probe. The Nap-Lyso-Ph-OH probe was constructed according to the ICT mechanism and exhibited sensitivity to both polarity and viscosity. The probe exhibited low cytotoxicity, a large Stokes shift, excellent selectivity and photostability. The probe was successfully used for labelling and imaging of lipid droplets and lysosomes in cells and zebrafish. Interestingly, we used tobacco seedling cells to explore the ability of Nap-Lyso-Ph-OH for imaging lipid droplet labelling in plant cells.

Naked-eye detection of plant viral disease using polymerase chain reaction amplification and DNAzyme.

Plant viral diseases can seriously affect the yield and quality of crops. In this work, a convenient and highly sensitive biosensor for the visual detection of plant viral disease is proposed by the PCR-induced generation of DNAzyme. In the absence of nucleic acid for a target plant virus, the primers prohibited the production of G-quadruplex by forming a hairpin structure. However, PCR amplification occurred and generated a number of specific PCR products with free G-quadruplex sequences at both ends in the presence of the target cDNA. A catalytically active G-quadruplex DNAzyme was formed with the help of K+ and hemin, resulting in the formation of colored products visible to the naked eye and a strong absorbance by the addition of ABTS2- and H2O2. The absorbance and the logarithm of target cDNA concentrations showed a good linear relationship in the range of 10 fM-1.0 nM, with a linear regression equation of A = 0.1402 lg c + 0.3761 (c: fM) and a detection limit of 0.19 fM. This method was successfully applied to the analysis of emerging tobacco mosaic virus (TMV) infections in tobacco leaf samples collected in the field due to its flexibility and convenience, indicating a potential application for the early detection of plant viral disease.

Effect of Steam Explosion on the Structure, Chemical Composition and Pyrolytic Volatile Composition of Tobacco Waste

Tobacco stems are an important part of the tobacco leaf, containing a lot of cellulose, result in the low utilization of tobacco leaves in cigarettes. The pre-treatment technology of tobacco stems is a key to increase the utilization of tobacco stems. In this work, steam explosion treatment was carried out on tobacco stems with different moisture contents containing 2%, 5% and 10%, respectively. The steam explosion is carried out under different pressure and pressure holding time conditions, the changes in physical structure, chemical composition and pyrolysis volatiles of tobacco stem was investigated. The results indicate that steam explosion has a negligible impact on the crystalline structure of cellulose and low-order polysaccharide crystals, while it has an obvious effect on KCl. Steam explosion increases the specific surface area by destroying the amorphous structure on tobacco stems. The contents of chemical constituents and pyrolytic volatiles indicate that there is a significant leaching effect of lignin, but no change in cellulose content. Soluble polysaccharides, the main component of hemicellulose, were converted into pyrolytic volatiles e.g., furfural and furfuryl alcohol. In conclusion, the technology of steam explosion promotes the leaching, decomposition and chemical transformation of amorphous structures on tobacco stems, thus destroying the structure of the cell walls.

Flue-cured tobacco leaf grade detection through multi-receptive field features fusing adaptively and dynamic loss adjustment

Flue-cured tobacco leaf grade detection through multi-receptive field features fusing adaptively and dynamic loss adjustment

Deep learning-based intelligent control of moisture at the exit of blade charging process in cigarette production

Abstract Currently, in the production of cigarettes in the blade, charging export moisture control means is relatively single and can not effectively guarantee the excellent quality of cigarette filament. In this paper, first of all, the working principle of the tobacco blade charging machine is introduced, and the moisture of the tobacco leaf for the charging machine is dynamically analyzed, and the influence of the return air temperature control of the charging machine on the export moisture of the blade charging process is explored. Secondly, based on the traditional PID controller, an adaptive fuzzy PID controller is established by combining adaptive fuzzy rules, and then the stacked noise-reducing self-encoder in deep learning is combined with the adaptive fuzzy PID control to design the intelligent control structure of export moisture of leaf charging process. Finally, the effectiveness of export moisture intelligence control, process capability index, and the effect before and after application were analyzed in controlled experiments, respectively. The results show that the difference between the predicted value and the real value of blade export moisture in this paper’s method is only 0.5%, and the process capability index of this paper’s method is improved by 1.48 compared with the PID controller, and it can control the temperature of the return air of the charging machine in the range of 56.86℃~57.21℃. The intelligent control method of export moisture introduced by deep learning can accurately control the export moisture of the leaf dosing process, which effectively ensures the quality of tobacco filament making.

Multi-objective Optimization Algorithm Research on Tobacco Leaf Blend Formulation Design

Multi-objective Optimization Algorithm Research on Tobacco Leaf Blend Formulation Design

IS THE TOBACCO INDUSTRY IN MACEDONIA A SOCIALLY RESPONSIBLE SEGMENT?

Corporate social responsibility refers to the focus, to the voluntary corporate action of business entities, not only to profit, but also to respect for social goals and protection of the ecological environment, and thus above all the health of people, and the healthy maintenance of living and plant life in nature. In that regard, the framework convention on tobacco control also refers, where specifically in Article 5.3 many specifics are highlighted, among which, that the tobacco industry carries out activities to distance its image from the deadly nature of the tobacco products it produces and sells, and at the same time, to influence the raising of awareness for the preservation of public health. British American Tobacco (BAT) claims that “tobacco cultivation plays an important and positive role in livelihoods, helping to improve well-being and increase resilience ie. the tenacity of tobacco farmers and workers." Also, the larger companies of the tobacco industry emphasize the application of the Strategy of Corporate Social Responsibility, in order to improve their reputation, image and credibility, and to present themselves as good corporate entities, which are also aimed at supporting projects in the field of the arts, higher education, reducing hunger and improving the nutrition of the poor population, certainly, by separating financial resources from their realized profits. In the paper, in addition to a brief elaboration of the significance of the application of Corporate Social Responsibility in the tobacco industry, quantitative data on tobacco production in the world, individual countries and Macedonia are presented.

Determinants of Tobacco Cultivation and the Associated Impacts of Adoption in Hurungwe District, Zimbabwe

Determinants of Tobacco Cultivation and the Associated Impacts of Adoption in Hurungwe District, Zimbabwe

Electronic systems for monitoring tobacco cultivation

Electronic systems for monitoring tobacco cultivation

Climatic Analysis and Zoning for Quality Flue-Cured Tobacco Cultivation in Qixingguan District

Climatic Analysis and Zoning for Quality Flue-Cured Tobacco Cultivation in Qixingguan District

Bacterial and fungal communities regulated directly and indirectly by tobacco-rape rotation promote tobacco production.

Tobacco continuous cropping is prevalent in intensive tobacco agriculture but often leads to microbial community imbalance, soil nutrient deficiency, and decreased crop productivity. While the tobacco-rape rotation has demonstrated significant benefits in increasing tobacco yield. Microorganisms play a crucial role in soil nutrient cycling and crop productivity. However, the internal mechanism of tobacco-rape rotation affecting tobacco yield through microbe-soil interaction is still unclear. In this study, two treatments, tobacco continuous cropping (TC) and tobacco-rape rotation (TR) were used to investigate how planting systems affect soil microbial diversity and community structure, and whether these changes subsequently affect crop yields. The results showed that compared with TC, TR significantly increased the Shannon index, Chao1 index, ACE index of bacteria and fungi, indicating increased microbial α-diversity. On the one hand, TR may directly affect the bacterial and fungal community structure due to the specificity of root morphology and root exudates in rape. Compared with TC, TR significantly increased the proportion of beneficial bacterial and fungal taxa while significantly reduced soil-borne pathogens. Additionally, TR enhanced the scale and complexity of microbial co-occurrence networks, promoting potential synergies between bacterial OTUs. On the other hand, TR indirectly changed microbial community composition by improving soil chemical properties and changing microbial life history strategies. Compared with TC, TR significantly increased the relative abundance of copiotrophs while reduced oligotrophs. Notably, TR significantly increased tobacco yield by 39.6% compared with TC. The relationships among yield, microbial community and soil chemical properties indicated that planting systems had the greatest total effect on tobacco yield, and the microbial community, particularly bacteria, had the greatest direct effect on tobacco yield. Our findings highlighted the potential of tobacco-rape rotation to increase yield by both directly and indirectly optimizing microbial community structure.

SUITABILITY ANALYSIS OF TOBACCO CULTIVATION TECHNIQUE IN NAGARI BARUAH GUNUNG

The problem with tobacco farmers in Nagari Baruah Gununang is a productivity. Tobacco productivity is below national productivity due to cultivation techniques that do not comply with standards. The research method uses suitability analysis to assess the level of suitability of cultivation techniques used by farmers in Nagari Baruah Gunuang. The level of suitability is analyzed using check sheet based on a likert scale by comparing farmers actual and standards cultivation. The research results show that suitability level of tobacco cultivation technique is 70%. This means that the cultivation techniques used by farmers are still classified as almost in accordance with standards. The stages of cultivation that do not meet standards are planting; fertilization; and weeding, watering and loosening. The problems that cause non-compliance with this standard are close spacing of plants, lack of use of manure and dolomite, inappropriate dosage and type of chemical fertilizer, and insufficient watering and irrigation.

Study of Antimicrobial Quality of Tobacco Leaves Extract (Nicotiana tabacum) on Aeromonas hydrophila and Escherichia coli.

ABSTRACT: Tobacco is a perennial herb and its belong to family Solanaceae. Tobacco leaves have some antimicrobial substance such as alkaloids, phenol and some essential oils. The leaves extract of this plant show some microbial Quality and influence on both gram positive and gram negative bacteria. The aim of this work to determine the effectiveness of tobacco (Nicotiana tabacum) leaves extract in inhibiting on Aeromonas hydrophila and Escherichia coli. The antibacterial test performed on Mueller Hinton Agar (MHA) media. Test result indicate the leaves extract of tobacco showed positive result in Aeromonas hyrdophilla. Tobacco extract can inhibit Aeromonas hyrdophilla growth and a clear zone are found, in case of E. coli it showed negative result in both the cases. Tobacco extract cannot inhibit E. coli growth and the diameter of the inhibitory zone is not clear. The result shown that tobacco leaf extract can be source of an active antimicrobial substance.

استخدام الاعشاب في العلاجات البيطرية لمواشي المربين المحليين

The herbs have a huge role in development of pharmacology besides the role of veterinary medicine in development of human medicine The study aimed to evaluate the therapeutic efficacy of pomegranate peel and leaves of both thuja and tobacco in 5 cows, 15 sheep and 15 goats were brought to veterinary clinics on the outskirts of Baghdad suffering from health problems in the skin, upper respiratory tract and digestive system. Cases were treated topically or orally as a powder, aqueous solution, or ointment. The results showed that 66% of cases were cured. The cure rate was related to the severity of the infection or lesion, course of treatment, routes of administration and herbal formula. Also, the results did not show any side effects of the herbal during the treatment period. Pour- on using of tobacco leaf infusion for lice-infected goats gave a better result than in sheep. The thuja and peels of pomegranate have proven to healing as an ointment for wounds, sores and blisters. Also, the aqueous solution or powder of thuja and peel of pomegranate have proven effective in reducing symptoms in the digestive system and upper respiratory disorders. Both ointments of thuja and pomegranate gave acceptable results for the treatment of warts (papilloma). Therefore, the farmer can use these herbs in emergencies. Recommendation is conducting more clinical veterinary studies on pomegranate, thuja, and tobacco leaves and their medicinal propriety. Thus, the field of veterinary medicine is the best field for reviving biopharmaceutical research and comparative medicine.

Preliminary Evaluation of Onion Bulb and Tobacco Leaf Extracts for Insect Control and Yield of Cowpea in the Southern Guinea Savanna of Nigeria

The study evaluated the effects of onion (Allium cepa) bulb and tobacco (Nicotiana tabacum) leaf extract on the insect pests and yield components of cowpea. The extracts were tested on number of the flower thrips, Megalurothrips sjostedti Trybom, the pod-sucking bug, Clavigralla tomentosicollis Stal, the pod borer, Maruca vitrata (F.) and cowpea aphid, Aphis craccivora Koch. Also evaluated were the dry seed weight, 100-seed weight, pod length, number of pods per plant, number of seeds per pod, pod load, pod damage and pod evaluation index. Field experiment was conducted in the Faculty of Agriculture, University of Ilorin, Ilorin, Nigeria. Plots were laid out in a randomized complete block design with three replications. Treatments included 10% (w/v) of each of onion bulb and tobacco leaf extracts, synthetic insecticide (Cypermethrin 1 L/ha) and untreated control. Results revealed that cowpea treated with the plant extracts had significantly (p<0.05) lower insect pest population than plants in untreated plots which suffered greater insect pest attack. However, plots treated with Cypermethrin produced significantly higher number of seeds per pod than other treatments. Nevertheless, tobacco leaf extract produced significantly (p<0.05) heavier 100-seed weight than other treatments including the control. It could be concluded that the plant extracts had potential to replace the synthetic insecticide for control of insect pests of cowpea, thus ensuring environmental safety.

Efficacy of Bio-Rational Insecticides in Controlling Thrips in Summer Country Bean

Aims: To study the efficacy of biorational insecticides in controlling thrips in summer country bean.
 Study design: The study used a single component Randomized Complete Block Design (RCBD) with three replications.
 Place and Duration of Study: The experiment was conducted in the central farm of Sher-e-Bangla Agricultural University, Dhaka, Bangladesh from March to May 2021.
 Methodology: The experiment consisted of seven treatments and was laid in a single factor Randomized Complete Block Design (RCBD) with three replications. The experiment consists of the following management practices: T1=Spraying Neem oil @ 6 ml/L of water at 7 days interval, T2=Spraying Neem leaf extract @ 200 mg/L of water at 7 days interval, T3= Mehgoni leaf extract @ 200 mg/L of water at 7 days interval, T4=Tobacco leaf powder @ 30 mg/L of water at 7 days interval, T5= Spraying Spinosad @ 0.4 ml/L of water at 7 days interval, T6=Spraying Biotrin @1.4 ml/L of water at 7 days interval and T7= Untreated control.
 Results: Among the seven (7) treatments the lowest number of thrips (2.50 plant-1) was observed in T5 (Spraying Spinosad @ 0.4 ml/L of water at 7 days interval), while highest number of thrips (16.67 plant-1) was observed in T7 (Untreated control). The highest percentage of thrips reduction over control (85.50%) was observed in T5 (Spraying Spinosad @ 0.4 ml/L of water at 7 days interval) treatment. The present study revealed that the lowest number of thrips as insect pest and increased plant, leaf, inflorescence and pod infestation, different yield contributing characters and yield of country bean might be obtained by using the treatment T5 (Spraying Spinosad @ 0.4 ml/L of water at 7 days interval) besides T6 (Spraying Biotrin @ 1.4 ml/L of water at 7 days interval) performed as second best treatment whereas, T7 (Control) and T3 (Mehgoni leaf extract @ 200 mg/L of water at 7 days interval) may protect the country bean at lowest rate in summer.
 Conclusion: It can be concluded that, spraying of Spinosad @ 0.4ml/L of water at 7 days interval as a bio-rational management practice for thrips population control for country bean variety BARI Sheem-4 in summer reduced the infestation of thrips at the most satisfactory level.

Effects of Soil Properties on Pb, Cd, and Cu Contents in Tobacco Leaves of Longyan, China, and Their Prediction Models.

Longyan City in Fujian Province is one of China's top-quality tobacco-producing areas and plays an essential role in local economic development. To determine the correlation between heavy metal content in tobacco leaves and soil factors, soil physical and chemical properties and heavy metal contents of lead, cadmium, and copper in tobacco leaves were measured and analyzed by the correlation regression method. The content of lead, cadmium, and copper in soil was determined using hydrochloric acid extraction-AAS and graphite furnace atomic absorption spectrometry. Inductively coupled plasma-mass spectrometry was used to determine heavy metal in tobacco leaf. The findings revealed that the average concentrations of lead, cadmium, and copper in the soil were 12.1, 0.092, and 3.88 mg/kg, respectively. In contrast, the average levels of lead, cadmium, and copper in tobacco leaves were 2.33, 4.89, and 4.35 mg/kg, respectively. The cadmium enrichment coefficient of 54.3 was higher than lead and copper, indicating a greater health risk. Soil pH value was negatively correlated with lead content in tobacco leaf, while potassium and phosphorus nutrient levels were negatively correlated with copper content. In contrast, a positive correlation was established between the presence of organic matter with cadmium content in tobacco leaves. The prediction models of lead, cadmium, and copper in tobacco leaves can be expressed by the regression equation corresponding to each heavy metal as follows: YPb=2.33 - 0.005∗ XK+0.007∗XN - 0.271∗XpH+0.065∗XPb (R2 = 0.787), YCd=1.55+0.012∗XOM - 0.014∗XCu+34.6∗XCd (R2 = 0.891), and YCu=4.64 - 0.029∗XP - 0.007∗XK+0.245∗XCu (R2 = 0.724), respectively. The prediction models above provide an effective predictive tool for assessing heavy metal risk in tobacco leaves using soil properties in the study area.

Sensitive Determination of Carbendazim in Tobacco Leaves by Surface Enhanced Raman Spectroscopy (SERS) Using a Silica – Silver Nanoparticle Substrate and QuEChERs Pretreatment

A sensitive surface enhanced Raman spectroscopy (SERS) substrate based on SiO2@Ag core-shell with a QuEChERs pretreatment method were constructed for the determination of carbendazim residue in tobacco leaves. The SERS substrate was formed by self-assembly of monolayer homogeneous SiO2 nanospheres onto silicon wafer following by vacuum thermal evaporation of silver film. The QuEChERs pretreatment method was used to eliminate the interfering effects of pigments and organic acids in tobacco leaves. The results for carbendazim in tobacco leaves show the limit of detection (LOD) of the SERS substrate was 0.1 mg/kg and the recovery rate was from 82.56% to 95.93%. The proposed low-cost sensitive SERS substrates provide potential application for tobacco safety monitoring.

Identification of CNN hyper-parameters for tobacco leaf quality classification on Nvidia Jetson Nano

At the moment, there are some inaccuracies in manual classification for tobacco leaf quality selection, which are influenced by some factors, such as human fatigue or poor lighting. These cases lead to the need for another method that is more consistent, faster and reliable. This research is an implementation of CNN (Convolutional Neural Network) in the classification of fresh tobacco leaves in terms of maturity grades. The primary objective is to develop an efficient CNN model capable to automize the classification of tobacco leaves into three maturity criteria: immature, mature, and old. This methodology consists of some key factors, including color thresholding strategies to purge the noise from the background, Basic Image Manipulation approaches, the systemized screening of different input sizes, and CNN models to enhance the results. The result of this research proves that the developed CNN model has 97.9 % accuracy achieved following 200 training sessions. The model is trained on a dataset comprising 1,249 fresh leaf photos, with a balanced 80:10:10 for train, validation and test ratio. However, the study emphasizes that the CNN model has successfully supported the tobacco leaf discrimination on a Jetson Nano Single-Board Computer with a Graphic Processing Unit (GPU). The study extends beyond the mere theoretical contribution to practical applications in sorting “Gagang Rejeb Sidi” tobacco leaf, the highest quality tobacco variety in South Malang, East Java, Indonesia. Classification using a webcam as an input device shows the fastest processing time of 203.17 ms and the maximum is 1,363 ms. This CNN model algorithm will be applied to a tobacco leaf selector machine, which has a high-speed conveyor and a three-position selector arm. The machine will be operated close to the field in post-harvest time under uniform lighting conditions. Overall, the result of this research is highly relevant in terms of the short duration and accuracy for understanding the commodity classification. It provides a new angle toward speeding up the classification process and improving Indonesian tobacco quality

Tobacco Cultivation Regimentation: An Ethnographic Treatise from Pagergunung Village, Bulu, Temanggung

Indonesia, as the sixth largest tobacco-producing country in the world, has participated in the Framework Convention on Tobacco Control (FCTC) by issuing compromise regulations through Government Regulation (PP) No. 109 of 2012. This regulation regulates restrictions on tobacco production and distribution of products made from tobacco raw materials. Furthermore, PP No. 109 of 2012 determines the increases in tobacco excise rates and the determination of limits on nicotine levels also sparked unrest among tobacco farmers in the Temanggung area. This article attempts to explain the insistence of farmers, especially in the eastern slope of Mount Sumbing, Pagergunung Village, Bulu District, Temanggung, in maintaining tobacco cultivation even though they are faced with an anti-tobacco campaign from the FCTC. The continued cultivation of tobacco can be understood as a form of regimen of cultivated plants. This article is an anthropological study with a regimentation theoretical perspective and ethnographic approach as a research method which presents the research results. From this research, it is known that the cultivation regimen is controlled by mythology, tobacco trade tricks, and the pretext of tradition as a socio-cultural surplus.