Abstract
ABSTRACT: Tobacco is a perennial herb and its belong to family Solanaceae. Tobacco leaves have some antimicrobial substance such as alkaloids, phenol and some essential oils. The leaves extract of this plant show some microbial Quality and influence on both gram positive and gram negative bacteria. The aim of this work to determine the effectiveness of tobacco (Nicotiana tabacum) leaves extract in inhibiting on Aeromonas hydrophila and Escherichia coli. The antibacterial test performed on Mueller Hinton Agar (MHA) media. Test result indicate the leaves extract of tobacco showed positive result in Aeromonas hyrdophilla. Tobacco extract can inhibit Aeromonas hyrdophilla growth and a clear zone are found, in case of E. coli it showed negative result in both the cases. Tobacco extract cannot inhibit E. coli growth and the diameter of the inhibitory zone is not clear. The result shown that tobacco leaf extract can be source of an active antimicrobial substance.
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