The effect of vitamin C toward the improvement of spermatogenesis and spermatozoa quality on mice (Mus musculus L.) after the addition of tobacco extract (Nicotiana tabacum L.)

Abstract

The aim of this research was to describe the ability of vitamin C in improving the spermatogenesis and spermatozoa quality of mice after the treatment of tobacco (Nicotiana tabacum L.) extract. The framework of the research was that it was already known that vitamin C was one of the anti-oxidant, which was able to counter free radical of the nicotine obtained in tobacco extract in improving the spermatogenesis. The research would be conducted by grouping the mice into 6 groups, and each group consists of 4 repetitions in 35 days. The first group was as control, the second group was treated with C vitamin in the dosage of 0.024 mg/g of body weight, the third group was treated with tobacco extract in the dosage of 0,121 mg/g of body weight, the fourth group was treated with tobacco extract in the dosage of 0,121 mg/g of body weight and C vitamin of 0,012 mg/g of body weight, the fifth group was treated with tobacco extract in the dosage of 0,121 mg/g of body weight and C vitamin of 0,024 mg/g of body weight, and the sixth group was treated with tobacco extract in the dosage of 0,121 mg/g of body weight and C vitamin of 0,036 mg/g of body weight. Based on the data analysis, it can be concluded that tobacco extract treatment could decrease spermatogenesis quality and spermatozoa quality of mice (Mus musculus L.). C Vitamin could improve spermatogenesis and spermatozoa quality of mice after tobacco extract treatment. The optimal dosage of C vitamin treatment to improve the spermatogenesis and the quality of spermatozoa after the addition of tobacco extract treatment was 0,024 mg/g of weight.