Tobacco Research Articles

SISTEM BAGI HASIL DALAM AKAD MUZARA’AH PADA PETANI TEMBAKAU DI SUMBER MALANG SITUBONDO

Plalangan Village is one of the villages where the majority of the livelihood is farming and in which there are many methods used to manage the land owned, ranging from managing it yourself to managing it by other people, one of the contracts used is the muzara'ah contract. The muzara'ah contract is a cooperative agreement between the land owner and the cultivator where the seeds to be planted come from the land owner. The agreement in this contract is usually done verbally without any written agreement with the distribution of the results agreed at the beginning of the agreement, however, in this distribution of results the cultivator is still waiting because the land owner is still looking for buyers who will buy tobacco, so this is the background for this research. . The type of research used is field research, that is, researchers go directly into the field to conduct research so they can see and understand the objects to be discussed. The results of this research show that the profit sharing system in the muzara'ah contract for tobacco farmers in Plalangan Village is that the distribution of the results is agreed upon by both parties at the beginning of the contract. What usually happens is that half is divided for the land owner and the other half for the cultivator, after deducting costs. -cost incurred. Meanwhile, the muzara'ah contract carried out by the people of Plalangan Village, Sumber Malang District, Situbondo Regency is valid because it has fulfilled the pillars and requirements of the muzara'ah contract and is in accordance with the principles of Islamic economics, namely the principle of monotheism, the principle of representation, the principle of justice, the principle of tazkiyah, and the principle of al-falah.

Effect of Soil Tillage Systems and Crop Seasonality on the Sediment Geochemical Properties Transferred into a Headwater: A Case of Study in Tobacco Plantation

ABSTRACT The global impacts of agricultural land conversion on soil erosion and pollution, particularly in tobacco cultivation areas, are well-recognized as significant contributors to soil degradation. These areas are identified as hotspots for environmental concerns due to practices that lead to increased erosion and pollution. From this perspective, this case of study explores fine sediment samples from two areas with tobacco cultivation under different tillage systems and seasonal variations, transport into a headwater, and evaluates, on a local scale: (1) the impact of tillage systems on the geochemical signature of sediments; (2) if whether crop seasonality affects these sediment geochemical signatures. The Conventional Ridge Tillage (CRT) system involves extensive soil exposure and machinery for soil management, while the Mulch Ridge Tillage (MRT) system prioritizes soil conservation and relies on herbicides for weed control. The analytical methodology used to assess the sample element characteristics was Energy Dispersive X-ray Fluorescence (EDXRF). It was applied on the twenty fine sediments (ten of harvest and ten of inter-harvest season of tobacco) to quantitatively assess their inorganic composition. Additionally, Pearson correlation analysis, Hierarchical Cluster Analysis (HCA), and Principal Component Analysis (PCA) were applied on the EDXRF data to highlight the similarities and, thus, providing information to assess the complex data clustering patterns. As a result, the sediment compositions from the two studied soil systems are not similar. The PCA showed that the CRT sediments are characterized by the P, S, K, Ca, and Mn content, presenting a geochemical signature related to manure and fertilizer compared to the MRT, which is correlated with Al, Ti, Fe, Cu, and Zn contents, exhibiting a geochemical signature characterized by the natural soil composition. Therefore, the sediment geochemical signatures might be affected by two phases in the study area: a) tillage system characteristics and b) seasonal soil erosion. These findings underscore the importance of managing soil nutrients to mitigate soil pollution and nutrient exportation to aquatic systems. Moreover, the results emphasize the recommendations for sustainable agricultural practices in tobacco-growing areas to protect environmental quality.

Factors Affecting Access to Agricultural Inputs among Smallholder Tobacco Farmers in Kahama District, Tanzania

Appropriate application of agricultural inputs plays a significant role in offsetting the adverse effects of climate change on agriculture including in tobacco production. Use of agricultural inputs has been reported to improve quantity, quality and market value of tobacco worldwide. In view of that, this study was undertaken to assess the factors affecting access to agricultural inputs among tobacco smallholder farmers in Kahama District. Additionally, the study determined the effects of inputs applications on tobacco production. The interview method using structured questionnaires was employed to collect primary data from a sample of 180 tobacco smallholder farmers obtained through random sampling. The data were analysed through descriptive and inferential statistics. Findings revealed that 77.2% of smallholder farmers had an access to agricultural inputs while 22.8% did not. Access to these agricultural inputs was through Agricultural Marketing Cooperative Societies (AMCOS) (100.0 %), fellow farmers (68.3%), and agro-input dealers (48.2%). Furthermore, findings showed that price (p=0.029), AMCOS membership (p=0.000), awareness (p=0.045), frequency of usage (p=0.000), credit accessibility (p=0.012) and farm size (p=0.048) were the significant factors that influenced smallholder farmers to access agricultural inputs. Moreover, the effects of agricultural inputs applications on tobacco production were; increase of tobacco leaf size (100.0%), resilience to pests and disease (97.3%), quality of produced tobacco (91.3%) and amount of produce (80.6%). The study concludes that most farmers have access to agricultural inputs, and that access is influenced by a number of important factors. There are several ways that inputs impact tobacco production. The study strongly suggests that in order to improve accessibility and input usage, farmers should be encouraged to join AMCOS and get access to credit.

Evaluation of Intercropping and Tobacco Leaf Extract for Management of Fruit Borer (Helicoverpa armigera) and Some Associated Beneficial Insects on Tomato in Eastern Hararghe, Ethiopia

Tomato production is challenged by different insect pests among these, fruit borer is the most destructing pests of tomato. To tackle these problems, the study was initiated with the objective to evaluate intercropping and tobacco extract on fruit borer and some associated beneficial insects on tomato in Eastern Hararghe. Head cabbage, common bean, onion, tobacco leaf extract and karate were used as treatments. The experiment was laid out in RCBD with four replications. From these results, significant differences were observed between treatments on larval population per plant and infestation per plant for all insect pests tested and also in population reduction. Tobacco leaf extract highly reduced the population occurrence and infestation level of fruit borer next to karate and followed by onion intercropping. The number of branches, number of fruits, and number of damaged fruits, marketable yield, non-marketable yield, and total yield also showed a significant difference (P < 0.05) among the treatments. The number of fruits per plant, marketable yield, and Total yield were the highest on onion intercrops followed by karate and tobacco extracts whereas the lowest number was observed from head cabbage intercrops. Maximum yield and yield traits were recorded on tomato onion intercropping compared to the rests of intercrops while the botanical extract comparably maximized the yields with Karate. Therefore, tomato-onion intercropping and tobacco leaf extract were used as the best alternatives to karate, the synthetic insecticides in the management of tomato fruit borer.

Study on the change of global ecological distribution of Nicotiana tabacum L. based on MaxEnt model.

Nicotiana tabacum L. (tobacco) has extremely high economic value, medicinal value, scientific research value and some other uses. Though it has been widely cultivated throughout the world, classification and change of its suitable habitats is not that clear, especially in the context of global warming. In order to achieve rational cultivation and sustainable development of tobacco, current (average from 1970-2000) and future (2070, average from 2061-2080) potential suitable habitats of Nicotiana tabacum L. were forecasted with MaxEnt model and ArcGIS platform based on 854 occurrence data and 22 environmental factors in this study. The results revealed that mean temperature of warmest quarter (bio10), annual precipitation (bio12), solar radiation in September (Srad9), and clay content (CLAY) were the four decisive environment variables for the distribution of Nicotiana tabacum L. Under current climate conditions, suitable habitats of Nicotiana tabacum L. were mainly distributed in south-central Europe, south-central North America, most parts of South America, central Africa, south and southeast Asia, and southeast coast of Australia, and only 13.7% of these areas were highly suitable. By the year 2070, suitable habitats under SSP1-2.6, SSP3-7.0, and SSP5-8.5 climate scenarios would all increase with the largest increase found under SSP3-7.0 scenario, while suitable habitats would reduce under SSP2-4.5 climate scenario. Globally, the center of mass of suitable habitats would migrate to southeast to varying degrees within Libya under four different climate scenarios. The emergence of new habitats and the disappearance of old habitats would all occur simultaneously under each climate scenario, and the specific changes in each area, combined with the prediction results under current climate conditions, will provide an important reference for the adjustment of agronomic practices and rational cultivation of Nicotiana tabacum L. both currently and in the future.

Benefits of Canavalia ensiformis, arbuscular mycorrhizal fungi, and mineral fertilizer management in tobacco production

Tobacco (Nicotiana tabacum L.) has long been vital to Cuban agriculture, with its products renowned for their quality. Cuban tobacco is grown in soils with a long history of continuous farming using traditional fertilization methods characterized by recommended doses of mineral fertilizers. This study aims to improve the nutrition resource strategy in tobacco cultivation to ensure high yields of superior-grade tobacco leaves with adequate quality and increase fertilization efficiency. With this goal, a field experiment evaluated the traditional method of fallow with alternatives of nutrient supply systems for the production of black tobacco in Ultic Paleustalf soils. The experiment utilized Canavalia ensiformis (Can) treated with a mycorrhizal inoculum (AMF) based on the Glomus cubense strain (INCAM-4) as a preceding green manure, combined with successive mineral fertilizations for tobacco during four growing seasons in a randomized block design with factorial arrangement. Canavalia presented a positive response to mycorrhizal inoculation, significantly increasing dry biomass production (87.34%, 129.96%), mycorrhizal colonization (26.90%, 103.66%), and spore production (26.79%, 52.52%) for Can and Can+AMF treatments respectively. A biplot analysis established a strong relationship between the biomass and mycorrhizal performance of Canavalia and the growth, yield, and mycorrhizal colonization of tobacco. The results indicate that inoculated Canavalia enhances mycorrhizal performance in successional tobacco, with Can+AMF significantly increasing mycorrhization of tobacco roots by (110.06%). Moreover, the combination of Can inoculate with AMF and 75% of the recommended mineral fertilization dose consistently produced the highest tobacco yields (42.06%), growth, and mycorrhizal activity across the four years while maintaining satisfactory combustibility. In this nutrition supply system, variations of the recommended fertilizer dose significantly decreased the percentage of mycorrhizal colonization. After four growing seasons using Can + AMF and Canavalia without inoculations, soil organic matter, and availability of exchangeable calcium, magnesium, and pH increased slightly without decreasing available phosphorus and potassium contents. Consequently, we conclude that Canavalia ensiformis, with an inoculum based on the Glomus cubense strain and 75% of the recommended dose of mineral fertilizers, provides an enhanced nutrition alternative system for black tobacco production.

MiRNA genetic variations associated with the predisposition of oral squamous cell carcinoma in central Indian population

The disease burden of Oral Squamous Cell Carcinoma (OSCC) is rising day-by-day and is expected to rise 62 % through 2035. The chewing of tobacco, areca nut, and betel leaf, poor oral hygiene, and chronic infection are common risk factors of OSCC, but genetic and epigenetic factors also contribute equally. MicroRNAs (miRNAs) are comprised of small, non-coding endogenous RNA that regulate a plethora of biological activities by targeting messenger RNA through degradation or inhibition. Single Nucleotide Polymorphisms (SNPs) in miRNA genes can regulate the development and progression of OSCC. The present study aimed to determine the association between SNPs in miRNA genes (miRSNPs) with the risk of OSCC. A case-control study involving 225 histo-pathologically confirmed OSCC cases and 225 healthy controls was conducted, where 25 miRSNPs were analyzed by iPLEX MassArray analysis. A SNP rs12220909 in MIR4293 showed a highly protective effect (CC vs GG, OR = 0.0431, 95%CI = 0.005–0.323, p = 3e-6). Whereas three SNPs, namely, rs4705342 in MIR143 (CC vs TT, OR = 2.25, 95%CI = 2.00–2.53, p = 0.0008), rs531564 in MIR124 (CC vs GG, OR = 24.18, 95%CI = 3.22–181.37, p = 3e-6), and rs3746444 in MIR499 (AA vs GG, OR = 2.01, 95%CI = 1.32–3.05, p = 0.001) were significantly associated with a higher risk of OSCC. Additionally, NanoString-based nCounter miRNA expression profiling revealed that miR-499a (Log2FC = −1.07), and miR-143 (Log2FC = −1.56) were aberrantly expressed in OSCC tissue. Taken together, the above miSNPs may contribute to the high incidence of OSCC in central India. However, further studies with large cohorts and ethnic stratification are required to validate our findings.

Determining nitrogen status using a universal critical nitrogen dilution curve for predicting flue-cured tobacco yield and quality

The critical nitrogen (Nc) dilution curve is considered an effective method for crop nitrogen (N) diagnosis, fertilization decision-making, and crop modeling. The Nc dilution curve is typically described using a power function curve (Nc = A1W−A2). This study established a universal Nc dilution curve and evaluated its potential application in flue-cured tobacco (Nicotiana tabacum L.) N diagnosis, yield, and quality regulation. Field experiments were conducted involving three tobacco varieties (Honghuadajinyuan, Yunyan87, and K326) and four N application rates (0 to > 90 kg N ha−1). The study was conducted in Southwest China from 2021 to 2022. The universal Nc curve obtained across all experimental data was 3.45 W−0.39. The parameter A2 was more susceptible to genotype and environment interactions than parameter A1. The uncertainty first rapidly decreased and then remained at a lower value as biomass increased. The relationship between leaf biomass and days after transplantation and accumulated growth degree was robust. The N nutrition index (NNI) and accumulated N deficiency effectively determined leaf N status and N demand, respectively. There was an inverse correlation between the NNI, leaf yield, and between the superior and medium leaf ratios. The quantitative relationship can be used to adjust the synergistic production of tobacco yield and leaf quality.

Arabidopsis and maize terminator strength is determined by GC content, polyadenylation motifs and cleavage probability

The 3’ end of a gene, often called a terminator, modulates mRNA stability, localization, translation, and polyadenylation. Here, we adapted Plant STARR-seq, a massively parallel reporter assay, to measure the activity of over 50,000 terminators from the plants Arabidopsis thaliana and Zea mays. We characterize thousands of plant terminators, including many that outperform bacterial terminators commonly used in plants. Terminator activity is species-specific, differing in tobacco leaf and maize protoplast assays. While recapitulating known biology, our results reveal the relative contributions of polyadenylation motifs to terminator strength. We built a computational model to predict terminator strength and used it to conduct in silico evolution that generated optimized synthetic terminators. Additionally, we discover alternative polyadenylation sites across tens of thousands of terminators; however, the strongest terminators tend to have a dominant cleavage site. Our results establish features of plant terminator function and identify strong naturally occurring and synthetic terminators.

Effects of Bacillus altitudinis inoculants on cigar tobacco leaf fermentation.

Microbial succession and metabolic adjustment during cigar tobacco leaf (CTL) fermentation are key factors to improve the quality and flavor of CTLs. However, the interactions in the above processes remain to be further elucidated. Bacillus altitudinis inoculants were added to the CTLs, and metagenomics and metabolomics were used to analyze the effects of the inoculants on regulating microbial succession, metabolic shift, and aroma production during fermentation. The addition of the inoculants reinforced the CTL macromolecule transformation and facilitated the aroma production efficiently, and the total aroma production was increased by 43% compared with natural fermentation. The omics analysis showed that Staphylococcus was a main contributor to fatty acid degradation, inositol phosphate metabolism, energy supply (oxidative phosphorylation), nutrient transport (ABC transporter and phosphotransferase system [PTS]), and aroma production (terpenoid backbone biosynthesis, phenylalanine metabolism, and degradation of aromatic compounds). Furthermore, Staphylococcus was positively correlated with TCA cycle intermediates (citric acid, fumaric acid, and aconitic acid), cell wall components, peptidoglycan intermediates (GlcNAc-1-P and UDP-GlcNAc), and phytic acid degradation products (inositol). The characteristics collectively showed Staphylococcus to be the most dominant in the microbial community at the genus level during microflora succession. The addition of the inoculants supplemented the nutritional components of the CTLs, enhanced the metabolic activity and diversity of bacteria such as Corynebacterium, improved their competitive advantages in the microflora succession, and facilitated the richness of microbial communities. Additionally, a metabolic shift in nicotine degradation and NAD + anabolism from Staphylococcus to Corynebacterium in fermentation with inoculants was first observed. Meanwhile, the significantly correlative differential metabolites with Staphylococcus and Corynebacterium were a metabolic complement, thus forming a completely dynamic fermentation ecosystem. The results provided evidence for CTL fermentation optimization.

Studi Fisika Tanah pada Budi Daya Tembakau (Nicotiana tabacum L) di Berbagai Kemiringan Lahan

The percentage of slope classes on a land has an impact on changes in soil physics. The purpose of this study is to evaluate the physical properties of soil planted with tobacco on various slopes in Kenagarian Situjuah Batua, Situjuah Limo Nagari District, Lima Puluh Kota Regency. The method used in this study was a survey; samples were taken purposively based on slope classes at slopes 8-15%, 15–25%, and 25–45% at depths of 0–20 cm and 20–40 cm. From each slope 3 repetitions were taken. The results showed that there were changes in soil physics properties in each slope class with the dominant texture of sandy clay loam. Organic matter was classified as high to low (18.77–3.63%), low soil buld density (0.66–0.34 g/cm3), and total pore space was classified as high (75.27–86.87%). The permeability of the soil was classified as very fast to somewhat slow (34.88–0.78 cm/h), and the aggregate stability index was somewhat steady to less steady (60.89–45.98%). It is recommended that tobacco farmers to make terrracing and cut the length of the slope so that it does not lose organic matter. Keywords: soil physical properties, slope level, tobacco plantation

Metabolomics Reveal the Chemical Characteristic of Cigar Tobacco Leaves During Air-Curing Process

Air-curing is a critical process in the production of cigar tobacco leaves. This process involves the vital degradation and transformation of macromolecular substances into micromolecular chemical compounds. In this study, three different varieties of tobacco leaves harvested from the Wuzhishan and Danzhou areas of Hainan, China were used as materials. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was employed to investigate the chemical changes before and after air-curing in the metabolites. Metabolic analysis revealed significant stepwise alterations in the tobacco leaf metabolome during the air-curing process. A total of 172 differential metabolites were identified as significant contributors in the biosynthesis pathways of flavonoids, flavone, flavonol, and other secondary metabolites, among the total analyzed 1841 metabolites obtained from the tobacco leaves. Notably, the contents of flavonoid metabolites, including kaempferol, rutin, chlorogenic acid, and quercetin, exhibited a significant decrease throughout the air-curing period. Correlation analysis indicated a strong association between the biosynthesis of flavonoids and the contents of chlorogenic acid and kaempferol, suggesting their role in regulation the formation of yellow and brown colors in tobacco leaves. These findings provide valuable insights for future investigations into the quality, commercial value, and chemical properties of cigar tobacco.

Biocontrol potential of plant growth-promoting rhizobacteria against plant disease and insect pest.

Biological control is a promising approach to enhance pathogen and pest control to ensure high productivity in cash crop production. Therefore, PGPR biofertilizers are very suitable for application in the cultivation of tea plants (Camellia sinensis) and tobacco, but it is rarely reported so far. In this study, production of a consortium of three strains of PGPR were applied to tobacco and tea plants. The results demonstrated that plants treated with PGPR exhibited enhanced resistance against the bacterial pathogen Pseudomonas syringae (PstDC3000). The significant effect in improving the plant's ability to resist pathogen invasion was verified through measurements of oxygen activity, bacterial colony counts, and expression levels of resistance-related genes (NPR1, PR1, JAZ1, POD etc.). Moreover, the application of PGPR in the tea plantation showed significantly reduced population occurrences of tea green leafhoppers (Empoasca onukii Matsuda), tea thrips (Thysanoptera:Thripidae), Aleurocanthus spiniferus (Quaintanca) and alleviated anthracnose disease in tea seedlings. Therefore, PGPR biofertilizers may serve as a viable biological control method to improve tobacco and tea plant yield and quality. Our findings revealed part of the mechanism by which PGPR helped improve plant biostresses resistance, enabling better application in agricultural production.

Analysis of Rewetting Characteristics and Mechanical Properties in Leaf Blades and Main Veins of Cured Tobacco Leaves

The moisture content and mechanical properties are crucial factors influencing the processing quality of cured tobacco leaves. Understanding the rewetting characteristics and mechanical properties of leaf blades and main veins is essential to for optimizing tobacco processing. This study measured the rewetting behavior and mechanical properties of leaf blades and main veins under varying temperature (20–40 °C) and relative humidity (RH, 50%–90%) conditions. Rewetting curves were obtained and dynamic models were constructed. Low-field nuclear magnetic resonance (LF-NMR) analysis was utilized to obtain information of relaxation time (T2), and correlation models between moisture state and mechanical properties were established. The results indicated that the rewetting rate of leaf blades and main veins decreased gradually with increasing moisture content, while higher temperature and RH enhanced the rewetting process. The rewetting time of leaf blades at 30 °C with 90% RH was reduced by 71.43% compared to that at 30 °C with 70% RH. For main veins, the rewetting time at 30 °C with 90% RH was shortened by 50.00% compared to 30 °C with 80% RH. The Peleg model was determined as the optimal dynamic model for describing the rewetting characters for leaf blades and main veins. Two moisture states were observed: strongly bound moisture and weakly bound moisture. The peak area of strongly bound moisture increased gradually, while the peak area of weakly bound moisture initially increased and then decreased. At the moisture content of 0.15 g/g, the strongly bound moisture content of leaf blades decreased by 7.75% compared to main veins, whereas the weakly bound moisture content of leaf blades increased by 3.04 times. However, the proportion of weakly bound moisture decreased despite the increasing proportion of strongly bound moisture. Furthermore, the relaxation time of leaf blades and main veins increased during rewetting. The mechanical properties analysis revealed a correlation between breaking force, strain, and elastic modulus with the content of strongly bound moisture. This study provides a theoretical basis for the development of improved rewetting processes in tobacco production.

Analysis of the Economic Potential of Tobacco Farmers for Community Welfare in Wungurejo Village, Ringinarum District, Kendal Regency

The potential for community welfare in Wungurejo Village, Ringinarum District, Kendal Regency requires alternative strategies for tobacco farming to reduce the risks posed. The government also needs to be actively involved in supporting holistic programs for tobacco farmers in order to achieve sustainable agricultural goals so that economic potential can determine the welfare of the community. This research is qualitative research using data collection methods in the form of interviews, surveys and observations and aims to analyze the economic potential of tobacco farmers on the welfare of the community in Wungurejo village.

Spectrophotometric and Chromatographic Determination of Alkaloids and Nicotine Contents in Lebanese Tobacco Leaves

Nicotiana Tabacum; the annual herb; known as Tobacco from the Solanaceae family was known for its alkaloids and especially nicotine (NCT) content; smoking products, insecticides, anthelminthic activity and clinically proven therapeutics are examples of its uses. Herein two different methods were applied in an attempt to quantify the total alkaloids and NCT content in Nicotiana tabacum cultivated in Lebanon. Total alkaloids were investigated through the formation of a complex with bromocresol green under a simple spectrophotometric method. Whilst HPLC-DAD was the choice for the quantitation of NCT levels. The column was Lichrospher select B (5 µm, 250x4 mm), the temperature was set at 29 ⁰C and the wavelength at 260 nm. The mobile phase consisted of 2 M O-phosphoric acid and methanol (60:40, v/v) using isocratic elution at 1 mL/min. A linear relationship was proved under both instruments. The extraction yield of alkaloid totum ranges between 2.1 ± 0.25 and 6.8% ± 0.58 and alkaloids contents range from 12.14 ± 2.01 to 53.12 ± 4.54 mg of AE/g of extract for Ghandouriyeh and Danniye samples respectively. On the other side among the different areas which cultivated Tobacco in Lebanon, Danniye was found to have the highest NCT concentration of dry weight (2.64%) while Al-Hissa possesses the lowest content (0.75%). Even if the results were generally similar to other countries, the study showed a difference in values from one region to another.

A Comprehensive Economic Analysis of Tobacco Farming Potential in Grobogan Regency, Central Java

This study aims to investigate the planning for the Tobacco Products Industrial Area (KIHT) development in Grobogan Regency, Central Java, based on economic analysis. KIHT is an area where the activities of the tobacco products industry are concentrated, equipped with infrastructure, facilities, and supporting facilities for the tobacco products industry. The study was designed with a survey approach, in-depth interview, and focus group discussion (FGD) with 100 key informants to obtain facts from the existing phenomena and find information factually. The number of key informants is based on the number all of tobacco numbers in Grobogan regency. The study, therefore, analyzes tobacco competitiveness, labor absorption, product market, and macroeconomic analysis. To sharpen the analysis, the study was supported with a questionnaire instrument to see how far tobacco farmers were prepared in KIHT development. Empirically, the results showed that tobacco farming absorbed 150 HOK/ha per season, greater than corn farming which only absorbed 52 HOK per season per ha and rice farming which absorbed 87 HOK per season per ha. It had high competitiveness with the oligopsony market structure. In addition, the market for tobacco products was experiencing a significant upward trend, and it is in accordance with the Cobweb Theorem law that the behavior of farmers will try to increase the planting area when there is a previous price increase. From the potential of tobacco products, Grobogan Regency received an excise duty of IDR 14.87 billion or 2% of the total excise duty of Central Java Province. This revenue-sharing fund will increase the regional fiscal capacity.

Direct Z-scheme CeO2/TiO2 heterojunction derived from tobacco leaf vein biotemplate for visible-light photocatalytic degradation of tetracycline hydrochloride

The development of visible-light-responsive biotemplated TiO2-based photocatalysts has been receiving much attention due to the pressing issue of antibiotic-residues in wastewater. In this work, tobacco leaf veins were utilized as a biotemplate to fabricate Z-scheme CeO2/TiO2 (GTS-CeO2/TiO2) photocatalysts via a solvothermal process followed by in situ calcination. Moreover, the microstructure units of the biotemplate-shaped photocatalysts can be regulated as required. Characterization and photocatalytic test results indicate that the biotemplated CeO2/TiO2 composites feature tight heterojunctions, which can effectively accelerate the separation and migration of photogenerated carriers for the degradation of tetracycline hydrochloride. The photodegradation efficiency of 20 ppm tetracycline hydrochloride under visible-light irradiation reaches 98.9 % over the GTS-CeO2/TiO2 in 120 min, with an apparent rate constant (Kappt) that is 2.9 times higher than that of CeO2/TiO2 synthesized without biotemplating. The enhanced photocatalytic performance is attributed to the construction of Z-scheme CeO2/TiO2 heterojunction, the presence of Ce4+/Ce3+, the unique morphology derived from the biotemplate, and the designed microstructure unit of the photocatalyst. The GTS-CeO2/TiO2 exhibits good stability and can be reused several times. The degradation pathways of TCH were primarily dominated by h+, ⋅OH, and ⋅O2-, leading to a reduction in the toxicity of intermediates. The degradation mechanism of tetracycline hydrochloride was also analyzed and proposed. This work provides a new inspiration for the preparation of visible-light photocatalysts to purify the environment in a more sustainable and environmentally friendly manner.

Additive patterns in near-infrared diffuse reflectance spectra: Implications for product formulation and analysis

In this study, we investigated the additive patterns observed in near-infrared (NIR) diffuse reflectance spectra within the context of food and pharmaceutical product formulation. Employing the Kubelka–Munk theory, we examined the linear correlation between spectra and concentration in polymer materials and tobacco powder samples. Our findings confirm the principle of spectral additivity in diffuse reflectance spectra, demonstrating a linear relationship when the sample scattering coefficient remains constant. Moreover, our results validate the feasibility of substituting actual mixed spectra with NIR additive spectra in tobacco leaf systems. This approach can potentially enhance formulation design, thereby improving efficiency and accuracy while expanding the scope and combination of formulation materials. Furthermore, this study offers a rapid, information-rich, and environmentally friendly alternative to traditional methods, with significant implications for the future of product formulation.

Genetic Diversity of Ralstonia solanacearum Causing Tobacco Bacterial Wilt in Fujian Province and Identification of Biocontrol Streptomyces sp.

Tobacco bacterial wilt is a highly destructive soilborne disease caused by the Ralstonia solanacearum species complex, exhibiting a significant risk to global flue-cured tobacco cultivation and resulting in substantial economic loss. In this study, 77 isolates were collected from three prominent flue-cured tobacco cultivation areas in Fujian, China (Nanping, Sanming, and Longyan), in 2021 and 2022. The isolated strains were classified through phylotype-specific multiplex polymerase chain reaction (Pmx-PCR) and physiological tests. The analysis showed that all the strains were associated with phylotype I, race 1, and biovar III. Subsequent phylogenetic analysis using partial egl gene sequences classified the 77 isolates into 5 distinct sequevars: 13, 15, 16, 17, and 34. Notably, a remarkable predominance of sequevar 15 was observed in Fujian Province, while sequevar 16 was first reported on tobacco in China, which was identified in other plants, expanding the understanding of its host range and distribution in the country. In addition, a Streptomyces strain extracted from the rhizosphere soil of tobacco was found to inhibit the growth of multiple sequevars of tobacco R. solanacearum, indicating its broad-spectrum antagonistic properties. Furthermore, pot experiments showed that the strain St35 effectively controlled tobacco bacterial wilt. The isolate St35 was conclusively identified as Streptomyces gancidicus according to the morphological and genetic features. In summary, the present study demonstrated the genetic diversity and distribution of tobacco R. solanacearum strains in the Fujian province of China, as well as the identification of a candidate biological control agent for the management of tobacco bacterial wilt.