Event Abstract Back to Event Tissue spheroids spreading on synthetic electrospun matrices Leandra Baptista1, Elisaveta Koudan2, Elena Bulanova2, Frederico D. Pereira2, Vladislav Parfenov2, Leonardo Boldrini1, Marcos A. Sabino3, Jaime Salazar3, Yubexi Correa3, Neudo Urdaneta3, Janaina A. Dernowsek4, Vladimir Kasyanov5, Rodrigo Rezende4, Usef Hesuani2, Jorge V. Da Silva4, José M. Granjeiro1 and Vladimir Mironov1, 2, 4 1 InMetro and UFRJ, Dimav, Brazil 2 3D Bioprinting Solutions, Russian Federation 3 Simon Bolivar University, Chemistry, Venezuela 4 Center for Information Technology Renato Archer, 3D Technologies, Brazil 5 Riga Stradins University and Riga Technical University, Latvia Aim: Tissue spheroids (chondrospheres) are already used in clinical practice for treatment of cartilage defects. However, potential washing out of some non-attached tissue spheroids strongly suggests that preliminary attachment and spreading of tissue spheroids on electrospun matrices could potentially eliminate this undesarable effect. In order to estimate potential of using electrospun matrices as a carrier for tissue spheroids we study kinetics of tissue spheroids spreading on electrospun matrices. Methods: The battery of electrospun matrices from different biomaterials including fluorescent electrospun matrices with the luminiscen Tris (8-Aluminium hydroxiquinolate III) has been fabricated using home-made and commercial electrospinning device (Yflow, Spain). The tissue spheroids (chondrospheres and desmospheres) have been biofabricated using micromolded non-adhesive hydrogel (Microtissue Inc, USA). The material properties of tissue spheroids have been estimated by tensiometry (Microsquisher, CellScale, Canada). The kinetics of tissue spheroids spreading has been evaluated using light and scanning electron microscopy. Results: Tissue spheroids attached and spread on tested elctrospun matrices. The kinetics of tissue spheroids spreading is determined by physico-chemical properties of tested electrospun matrices as well as by the material properties of tissue spheroids estimated by tensiometry. Initial pattern and density of tissue spheroids robotic placing determines the resulted thickness of tissue enginered constructs. Conclusions: The tissue spheroids attached and spreaded on different electrospun matrices. Electrospun matrices could be used as carriers for implantation of spreaded tissue spheroids. Moreover, GFP labelled tissue spheroids or fluorescent electrospun matrices could enable the real time quantitative recording of tissue spheroids spreading kinetics and be employed for high throughput screening tissue biocompatibility of synthetic electrospun biomaterials. Our special thanks to the brazilians FAPESP (2008/57860-3), CNPq and INCT-BIOFABRIS.