High throughput fabrication of cell spheroids by templating water-in-water Pickering emulsions

Anupam A. K. Das,Benjamin W. Filby,Deborah Legrande,Daniel A. Geddes,Vesselin N. Paunov

doi:10.1039/c7mh00677b

Anupam A. K. Das, Benjamin W. Filby + Show 3 more

Open Access

https://doi.org/10.1039/c7mh00677b

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Abstract

We report a simple technique for the high throughput generation of tissue spheroids based on the encapsulation of dispersed adherent cells in particle-stabilized water-in-water emulsions.

Highlights

We report for the first time a rapid and scalable production technique of tissue spheroids by trapping cells in droplets of water-in water Pickering emulsion stabilised by protein particles
The emulsion is formed of an aqueous solution of dextran (DEX) dispersed in a continuous phase of aqueous solution of polyethylene oxide (PEO)
The schematic of our cell spheroids fabrication method is shown in Figure 1 for adherent cells which are dispersed in the DEX phase

Summary

Introduction

We report for the first time a rapid and scalable production technique of tissue spheroids by trapping cells in droplets of water-in water Pickering emulsion stabilised by protein particles. The emulsion is formed of an aqueous solution of dextran (DEX) dispersed in a continuous phase of aqueous solution of polyethylene oxide (PEO). The schematic of our cell spheroids fabrication method is shown in Figure 1 for adherent cells which are dispersed in the DEX phase.

Results

Conclusion