Tissue Material Research Articles

Validitas Modul Pembelajaran Biologi Materi Jaringan Pada Tumbuhan Kelas XI. IPA MAN 1 Solok Selatan

A module is a type of teaching material that is packaged completely and systematically. MAN 1 Solok Selatan already has teaching materials, namely textbooks. In terms of the availability of textbooks at the school, it is not sufficient for the number of students, so not all students can borrow textbooks. There are shortcomings in the package book, namely that the material in the book is still minimal, as well as a lack of pictures of plant tissue. This research aims to produce a module on tissue material in plants for class XI. IPA MAN 1 Solok Selatan which is very valid and very practical. This research was conducted in the odd semester of the 2023/2024 academic year in class XI. IPA at MAN 1 Solok Selatan. This research is development research using a 4-D model which includes 4 stages, namely the define, design, develop and disseminate stages. This research was only carried out at the develop stage, namely validity testing. In the define stage, front end analysis is carried out (using questionnaires and interviews), student analysis, and task analysis starting from content structure analysis, concept analysis and learning objective analysis. The design stage is carried out by designing modules. In the develop stage, a validity test was carried out by filling out a questionnaire by 2 lecturers and 1 Biology teacher. Data were analyzed using percentage techniques and processed using descriptive analysis. The validity results by lecturers and teachers obtained an average of 91.81% with very valid criteria. It can be concluded that the module on plant tissue material for class XI IPA MAN 1 South Solok that was developed is very valid.

Endo-Surgical Force Feedback System Design for Virtual Reality Applications in Medical Planning

Virtual reality (VR) applications for surgical planning and mentoring are currently becoming more widespread. Within these fields of applications, haptic systems are crucial to help the understanding of the feedback provided by the tissue during the interaction with the surgical tool. The accuracy of the VR scenario is related to many aspects, among them the ability of the haptic device to provide the force feedback and thus the adoption of proper characterization and modeling of the soft tissue behavior during deformation. This paper presents the current development concerning a VR environment to be equipped with a force feedback haptic device to simulate and experience clamping in colorectal surgery. The reaction at the clamp handle is provided starting from a FEA of the contact between the surgical tool and the tissue, coupled with the kinematic analysis of the mechanism. Connecting the virtual scenario to a microcontroller board, the reaction force is applied to the the haptic device, shaped as a clamping tool handle, so that the user may experience the tissue impedence during clamping. By simulations made through Simscape, a concept is evaluated according to some simulation paths that involve common scenarios of the virtual reality experience (such as collision, partial or total clamping operation). The novelty of the research regards the system design oriented to increase the accuracy of the soft tissue material behaviour through a preliminary off-line dedicated FEA investigation. Through Simulink simulations, the system design is verified so that the VR system set-up may be carried out. The results highlighted: (a) a good correspondence among the theoretical study of the reference kinematism that the haptic device must reproduce; (b) the implementation of a bilinear material model, derived off-line from FEA, is suitable to capture hyperelastic and geometric non-linearities of the soft tissue; (c) the feasibility of the open loop haptic control in accordance to the explored kinematism. The final goal of the research is to propose a system design approach able to tune the VR experince for teaching and mentoring through a in-house set-up easy to be tailored.

Diagnostic informativeness of biopsy for chronic kidney disease in cats

The biopsy is a diagnostic method that allows in vivo sampling of kidney tissue material for histopathological examination to identify structural changes in the organ. The study aims to study the informativeness of intravital diagnosis of stage II chronic kidney disease (according to IRIS) in cats based on the results of histological and histochemical studies of biopsies. A kidney biopsy was performed on two cats aged 6 and 8 years. Research methods: histological (hematoxylin and eosin, Heidenhain test) and histochemical (PAS reaction, Gomori (PAMS)). The diagnosis of chronic kidney disease was made based on history, clinical symptoms, and blood and urine test results. Based on the biopsy, acute extracapillary serous-desquamative glomerulonephritis was established in cats with II-stage chronic kidney disease, which was characterized by vacuolar dystrophy of renal tubule epitheliocytes, desquamation of glomerular capsule epithelium. In addition, lymphoplasmacytic infiltration of the renal stroma, thickening of the Shumlyansky-Bowman capsule with significant deposition of PAS-positive substances, the collapse of the glomerular loops, and focal atrophy of the proximal tubules of the kidney pronounced extra capillary desquamation of the glomerular epithelium, segmentation of the vascular glomerulus, and dystrophy of single tubules were noted. The detected changes in kidney biopsies of cats in the II stage of chronic kidney disease are characteristic of this pathology, which makes it possible to develop and carry out effective treatment of animals promptly and improve their quality of life.

Biomimetic highly porous nanocellulose–nanohydroxyapatite scaffolds for bone tissue engineering

Wood-derived TEMPO-oxidized cellulose nanofibrils (CNFs) have potential as scaffolding for bone tissue engineering. Although biocompatible, the material lacks osteoconductive and appropriate mechanical properties. Incorporation of nano-hydroxyapatite (nHA) and modification of scaffold preparation methods could improve applicability. In this study, freeze-dried porous scaffolds were prepared using a range of nHA (0, 20, 33, 50%) and CNF compositions. Not only the microarchitecture but also the chemical composition of the scaffolds was studied. Osteoblast-like osteosarcoma derived cells (Saos-2) were cultured on the scaffolds and their responses (viability, attachment, proliferation, and osteogenic phenotype) to the different scaffolds were documented. The results show that incorporation of nHA influenced the microarchitecture, mechanical stiffness and surface properties of the scaffolds. Moreover, biological characterization demonstrated good cell viability in all the groups. However, the increase of nHA concentration beyond 20% does not offer further advantages. It is concluded that the incorporation of 20% nHA resulted in the widest and most biomimetic pore size distribution, increased surface roughness and improved protein adsorption. These changes in material properties enhanced cell spreading and the osteogenic gene expression of osteoblast-like cells seeded on the scaffolds. Moreover, 20% nHA warrants further investigation as a potential scaffolding material for bone tissue engineering.Graphical abstract

Tubular Nanoclay-Enhanced Calcium Phosphate Mineralization and Assembly to Impart High Stiffness and Antimicrobial Properties.

Achieving superior mechanical properties of composite materials in artificially engineered materials is a great challenge due to technical bottlenecks in the size and morphological modulation of inorganic nanominerals. Hence, a "bioprocess-inspired fabrication" is proposed to create multilayered organic-inorganic columnar structures. The sequential assembly of halloysite nanotubes (HNTs), polyelectrolytes (PAAs), and calcium phosphates (CaPs) results in organic-inorganic structures. PAA plays a crucial role in controlling the formation of CaP, guiding it into amorphous particles with smaller nanosizes. The introduction of HNT induces the assembly and maturation of CaP-PAA, leading to the formation of a highly crystalline hydroxyapatite. Poly(vinyl alcohol) was then woven into HNT-encapsulated hydroxyapatite nanorods, resulting in composite materials with basic hierarchical structures across multiple scales. The fabricated composite exhibits exceptional hardness (4.27 ± 0.33 GPa) and flexural strength (101.25 ± 1.72 MPa), surpassing those of most previously developed biological hard tissue materials. Additionally, the composite demonstrates effective antibacterial properties and corrosion resistance, attributed to the dense crystalline phase of CaP. This innovative approach showcases the potential of clay minerals, particularly HNT, in the advancement of biomaterial design. The outstanding mechanical and antimicrobial properties of clay-based composites make them a promising candidate for applications in hard tissue repair, offering versatility in biomedicine and engineering.

Clinical benefits of MRI-guided freehand biopsy of small focal liver lesions in comparison to CT guidance

ObjectivesTo compare clinical success, procedure time, and complication rates between MRI-guided and CT-guided real-time biopsies of small focal liver lesions (FLL) < 20 mm.MethodsA comparison of a prospectively collected MRI-guided cohort (n = 30) to a retrospectively collected CT-guided cohort (n = 147) was performed, in which patients underwent real-time biopsies of small FLL < 20 mm in a freehand technique. In both groups, clinical and periprocedural data, including clinical success, procedure time, and complication rates (classified according to CIRSE guidelines), were analyzed. Wilcoxon rank sum test, Pearson’s chi-squared test, and Fisher’s exact test were used for statistical analysis. Additionally, propensity score matching (PSM) was performed using the following criteria for direct matching: age, gender, presence of liver cirrhosis, liver lobe, lesion diameter, and skin-to-target distance.ResultsThe median FLL diameter in the MRI-guided cohort was significantly smaller compared to CT guidance (p < 0.001; 11.0 mm vs. 16.3 mm), while the skin-to-target distance was significantly longer (p < 0.001; 90.0 mm vs. 74.0 mm). MRI-guided procedures revealed significantly higher clinical success compared to CT guidance (p = 0.021; 97% vs. 79%) as well as lower complication rates (p = 0.047; 0% vs. 13%). Total procedure time was significantly longer in the MRI-guided cohort (p < 0.001; 38 min vs. 28 min). After PSM (n = 24/n = 38), MRI-guided procedures still revealed significantly higher clinical success compared to CT guidance (p = 0.039; 96% vs. 74%).ConclusionDespite the longer procedure time, freehand biopsy of small FLL < 20 mm under MR guidance can be considered superior to CT guidance because of its high clinical success and low complication rates.Clinical relevance statementBiopsy of small liver lesions is challenging due to the size and conspicuity of the lesions on native images. MRI offers higher soft tissue contrast, which translates into a higher success of obtaining enough tissue material with MRI compared to CT-guided biopsies.Key Points• Image-guided biopsy of small focal liver lesions (FLL) is challenging due to inadequate visualization, leading to sampling errors and false-negative biopsies.• MRI-guided real-time biopsy of FLL < 20 mm revealed significantly higher clinical success (p = 0.021; 97% vs. 79%) and lower complication rates (p = 0.047; 0% vs. 13%) compared to CT guidance.• Although the procedure time is longer, MRI-guided biopsy can be considered superior for small FLL < 20 mm.Graphical

Traveling wave solution and the stability of critical points of an enzyme-inhibitor system under diffusion effects: with special reference to dimer molecule

Enzymes are absolutely essential biological catalysts in human body that catalyze all cellular processes in physiological network. However, there are certain low molecular weight chemical compounds known as inhibitors, that reduce or completely inhibit the enzyme catalytic activity. Mathematical modeling plays a key role in the control and stability of metabolic enzyme inhibition. Enzyme stability is an important issue for protein engineers, because of its great importance and impact on optimal utility of material in biological tissues. In this outlook, we have first determined the existence of traveling wave solution for the enzyme-inhibitor system and then emphasized the stability of critical points that arise in the reactions. The study of traveling wave solution of an enzyme-inhibitor system with reaction diffusion equations involve quite complex mathematical analysis. The results obtained in this model indicate that the traveling wave solution may give a well explained method for improving enzyme kinetic stability. The present study will be helpful in understanding the stability of critical points of an enzyme-inhibitor system to give an idea about the inhibition of less stable enzymes. Moreover, the role of diffusion on the enzyme activity has been exhaustively discussed using mathematical tools related to eigen values and eigen function analysis.

Head-to-head comparison of [18F]-Flortaucipir, [18F]-MK-6240 and [18F]-PI-2620 postmortem binding across the spectrum of neurodegenerative diseases

We and others have shown that [18F]-Flortaucipir, the most validated tau PET tracer thus far, binds with strong affinity to tau aggregates in Alzheimer's (AD) but has relatively low affinity for tau aggregates in non-AD tauopathies and exhibits off-target binding to neuromelanin- and melanin-containing cells, and to hemorrhages. Several second-generation tau tracers have been subsequently developed. [18F]-MK-6240 and [18F]-PI-2620 are the two that have garnered most attention. Our recent data indicated that the binding pattern of [18F]-MK-6240 closely parallels that of [18F]-Flortaucipir. The present study aimed at the direct comparison of the autoradiographic binding properties and off-target profile of [18F]-Flortaucipir, [18F]-MK-6240 and [18F]-PI-2620 in human tissue specimens, and their potential binding to monoamine oxidases (MAO). Phosphor-screen and high resolution autoradiographic patterns of the three tracers were studied in the same postmortem tissue material from AD and non-AD tauopathies, cerebral amyloid angiopathy, synucleopathies, transactive response DNA-binding protein 43 (TDP-43)-frontotemporal lobe degeneration and controls. Our results show that the three tracers show nearly identical autoradiographic binding profiles. They all strongly bind to neurofibrillary tangles in AD but do not seem to bind to a significant extent to tau aggregates in non-AD tauopathies pointing to their limited utility for the in vivo detection of non-AD tau lesions. None of them binds to lesions containing β-amyloid, α-synuclein or TDP-43 but they all show strong off-target binding to neuromelanin and melanin-containing cells, as well as weaker binding to areas of hemorrhage. The autoradiographic binding signals of the three tracers are only weakly displaced by competing concentrations of selective MAO-B inhibitor deprenyl but not by MAO-A inhibitor clorgyline suggesting that MAO enzymes do not appear to be a significant binding target of any of them. These findings provide relevant insights for the correct interpretation of the in vivo behavior of these three tau PET tracers.

Platelet Proteome Reveals Novel Targets for Hypercoagulation in Pseudoexfoliation Syndrome.

Pseudoexfoliation syndrome (PEX) is characterized by the accumulation of abnormal extracellular matrix material in ocular and non-ocular tissues, including blood vessel walls. Clot-forming dysfunction might be responsible for venous thrombosis in PEX. We investigated global coagulation, the proteome, and functions of platelets in PEX patients and aimed to determine prognostic biomarkers for thrombosis risk in PEX. Peripheral blood was collected from PEX and retinal vein occlusion (RVO) patients, and age-sex matched controls. Viscoelastic hemostasis was evaluated by rotational thromboelastometry (ROTEM). Platelet markers (CD41, CD42, CD61, and CD62p) and endothelial markers (P-selectin, E-selectin, and von Willebrand factor) were investigated by flow cytometry and ELISA, respectively. The platelet proteome was analyzed by 2D fluorescence difference gel electrophoresis followed by mass spectrometry. Clot formation time (CFT) is significantly reduced in PEX patients compared to the controls (p < 0.05). P-selectin levels were higher in PEX patients than in controls (p < 0.05); E-selectin and von Willebrand factor remained unchanged. The monitorization of CFT by ROTEM, and soluble P-selectin, may help assess thrombotic risk in PEX patients. Proteomic analysis revealed differential expression of Profilin-1 in platelets. Profilin-1 regulates the stability of actin-cytoskeleton and may contribute to impaired platelet hemostatic functions. Increased P-selectin levels together with impaired coagulation dynamics might be responsible for the thrombotic events in PEX disease.

Recognition of pseudoinvasion in colorectal adenoma using spatial glycomics.

Pseudoinvasion (PI) is a benign lesion in which cancer is mimicked in the colon by misplacement of dysplastic glands in the submucosa. Although there are morphological clues, the discrimination of PI from true invasion can be a challenge during pathological evaluation of colon adenomas. Both overdiagnosis and underdiagnosis can result in inadequate clinical decisions. This calls for novel tools to aid in cases where conventional methods do not suffice. We performed mass spectrometry imaging (MSI)-based spatial glycomics analysis on a cohort of formalin-fixed paraffin-embedded tissue (FFPE) material from 16 patients who underwent polypectomy. We used this spatial glycomic data to reconstruct the molecular histology of the tissue section using spatial segmentation based on uniform manifold approximation and projection for dimension reduction (UMAP). We first showed that the spatial glycomic phenotypes of the different morphological entities separated as distinct clusters in colon tissues, we separated true invasion from the other morphological entities. Then, we found that the glycomic phenotype in areas with suspected PI in the submucosa was strongly correlating with the corresponding glycomic phenotype of the adenomatous colon epithelium from the same tissue section (Pearson correlation distance average = 0.18). These findings suggest that using spatial glycomics, we can distinguish PI as having a molecular phenotype similar to the corresponding surface epithelium and true invasion as having a different phenotype even when compared to high-grade dysplasia. Therefore, when a novel molecular phenotype is found in the deepest submucosal region, this may be used as an argument in favor of true invasion.

Finite element analysis of the cervical spine: dynamic characteristics and material property sensitivity study

The study aimed to investigate the dynamic characteristics of the cervical spine and determine the effect of the material properties of the cervical spinal components on it. A finite element model of the head-cervical spine was developed based on CT scan data, and the first six orders of modes (e.g. flexion-extension, lateral bending, and vertical, etc.) were verified by experimental and simulation studies. The material sensitivity study was conducted by varying elasticity modulus of cervical hard tissues (cortical bone, cancellous bone, endplates, and posterior elements) and soft tissues (intervertebral disc and ligaments). The results showed that increasing the elastic modulus of ligaments by 4 times increased the natural frequency by 77%, while increasing that of cancellous bone by 4 times only increased the natural frequency by 6%. In the axial mode, the cervical spine had not only axial deformation but also anterior-posterior deformation, with the largest deformation located at the intervertebral disc C6-C7. Decreasing the elastic modulus of a component in soft tissues by 80% increased modal displacement by up to 62%. The material properties of the intervertebral discs and ligaments had opposite effects on the modal displacement and deformation of the cervical spine. Low cervical discs were more susceptible to injury in a vertical vibration environment. Cervical spine dynamics were more sensitive to soft tissue material properties than to hard tissue material properties. Disc degeneration could reduce the range of vibratory motion of the cervical spine, thereby reducing the ability of the cervical spine to cushion head impacts.

Native ChIP: Studying the Genome-Wide Distribution of Histone Modifications in Cells and Tissue.

For the genome-wide mapping of histone modifications, chromatin immunoprecipitation (ChIP) followed by high-throughput sequencing remains the benchmark method. While crosslinked ChIP can be used for all kinds of targets, native ChIP is predominantly used for strong and direct DNA interactors like histones and their modifications. Here we describe a native ChIP protocol that can be used for cells and tissue material.

Biochanin A restored the blood-brain barrier in cerebral ischemia-reperfusion in rats.

Blood-brain barrier is a protective layer that regulates the influx and efflux of biological materials for cerebral tissue. The aim of this study was to investigate the effects of Biochanin A on cerebral histopathology and blood-brain barrier immunohistochemically. A total of 24 rats were assigned to three groups: sham, ischemia-reperfusion, and ischemia-reperfusion+Biochanin A. Ischemia-reperfusion was performed by occluding the left carotid artery for 2/24 h. Notably, 20 mg/kg Biochanin A was administered to rats for 7 days after ischemia-reperfusion. Blood was collected for malondialdehyde and total oxidant/antioxidant status analysis. Cerebral tissues were processed for histopathology and further for immunohistochemical analysis. Malondialdehyde content with total oxidant status value was significantly increased and total antioxidant status values were significantly decreased in the ischemia-reperfusion group compared with the sham group. Biochanin A treatment significantly improved scores in the ischemia-reperfusion+Biochanin A group. The normal histological appearance was recorded in the cerebral sections of the sham group. Degenerated neurons and vascular structures with disrupted integrity of the cerebral cortex were observed after ischemia-reperfusion. Biochanin A alleviated the histopathology in the cerebrum in the ischemia-reperfusion+Biochanin A group. Ischemia-reperfusion injury decreased the expression of blood-brain barrier in the ischemia-reperfusion group compared to the sham group. Administration of Biochanin A upregulated the blood-brain barrier immunoreactivity in the cerebrum by restoring blood-brain barrier. Cerebral ischemia-reperfusion caused an increase in oxidative stress and pathological lesions in the cerebrum. Biochanin A treatment restored the adverse effects of ischemia-reperfusion injury by restoring blood-brain barrier.

GENERAL REQUIREMENTS FOR THE ORGANIZATION OF MEALS FOR CHILDREN AND ADOLESCENTS IN RESTAURANTS

The article is devoted to the consideration of the organization of food for children and adolescents in restaurants. General requirements for the organization of nutrition for children and adolescents have been established, that the organization of nutrition for children and adolescents involves rational nutrition, a variety of dishes, taking into account dietary needs and restrictions, as well as creating comfortable conditions for their reception. Maintaining these requirements contributes to the formation of healthy eating habits and ensures the normal growth and development of children and adolescents. The peculiarities of children’s nutrition at different age periods are given, which indicate the need to adapt the diet and food products to the agerelated needs and capabilities of the child’s body, their eating habits, the ability to digest various products, as well as the specifics of growth and development. The relevance of the topic of nutrition among children and adolescents in modern conditions has been studied. The main factors affecting the nutritional disorders of children older than 1-2 years and adolescents of older age groups are considered. Recommendations were provided on the distribution of calories per day for older children and adolescents with 4 meals a day. Features of nutrition of children and adolescents in different age periods and daily physiological norms of nutrition for children of different ages are given. It has been established that in order to ensure healthy nutrition for children and adolescents, special attention should be paid to the composition of food, in particular to the presence of essential amino acids, which are the main building material for new cells and tissues. Recommendations are provided that are important for ensuring proper physical and mental development, health and well-being of this population category. In addition, it is important to teach children and adolescents the basics of healthy eating. Understanding the importance of a balanced diet and a healthy lifestyle can contribute to the formation of good habits that will be beneficial for their future. The practical significance of the obtained results lies in the opportunity to provide and help restaurants serving the younger generation and improve their conditions.

Stable Isotope Analysis of Antipatharian Diets from the Northwestern Gulf of Mexico

Antipatharians, or black corals, form a key component of the benthic fauna found throughout the Gulf of Mexico (GOM). In the northwestern GOM, they are a common member of the communities found on the natural relic coral—algal banks known as the South Texas Banks as well as on artificial reefs. They are known to provide important habitat for many ecologically and economically important species in the area but are relatively understudied. Here we present a study on their trophic ecology using stable isotope analysis of carbon and nitrogen from 2 species, Antipathes atlantica and Stichopathes luetkeni from a mesophotic artificial reef in close proximity to the South Texas Banks. We observed that a large proportion of the 2 coral’s diets was composed of detrital matter, likely driven by the continuous disturbance of the benthos by high velocity benthic currents common to the region that support a persistent nepheloid layer below 30 m. Both species also show a reliance on different members of the planktonic community, with A. atlantica consuming more micro and mesozooplankton compared to S. luetkeni. Comparative analysis between soft tissue and skeletal material also indicates that A. atlantica may change its diet on a seasonal basis, while S. luetkeni appears to rely on detrital matter consistently. This study represents the first assessment of the diets of these organisms and provides valuable insight on the trophic ecology of 2 common antipatharian corals in the GOM on natural and artificial reef ecosystems.

Tumor mineralization-based cancer diagnosis and therapy

Biomineralization is a phenomenon that involves the deposition of inorganic ions onto organic substrates, resulting in the formation of hard tissue materials. Tumor mineralization, on the other hand, encompasses two key aspects: tumor calcification and tumor iron mineralization. The occurrence of spontaneous tumor calcification and regional lymph node calcification in colorectal cancer, lung cancer, and glioblastoma has been established as a favorable prognostic factor in clinical settings. Building upon this understanding, we propose the concept and advance the development of a compound that artificially induces bionic mineralization around the surface of cancer cells. This process has demonstrated exceptional efficacy in inhibiting the growth and metastasis of cervical, breast, and lung tumors. Moreover, it has exhibited outstanding performance in the early-stage diagnosis of cancer. Consequently, we anticipate that this concept holds significant potential for cancer-targeted mineralization therapy and diagnosis, offering a novel avenue for the development of anticancer drugs.

Three-dimensional dynamic homogenous modeling: The biomechanical influences of leg tissue stiffness on pressure performance of compression biomedical therapeutic textiles.

Patient compliance and therapeutic precision of compression textiles (CTs) are frequently limited by the inaccurate pressure distributions along biological bodies in physical-based compression therapy. Therefore, the biomechanical influences of physiological tissue material characteristics of lower extremities on compression generations of CTs need to be explored systematically to improve pressure management efficacy. In this study, we developed three-dimensional (3D) homogenous finite element (FE) CT-leg systems to qualitatively compare the pressure diversities along lower limbs with different biomaterial tissue properties under each external compression level. Simultaneously, through the obtained leg circumferential displacement, a contact analysis model was applied to quantitatively explore the impact mechanisms of soft leg indentations on the pressure performance of CTs. Based on the experimental validation study, the proposed FE systems could be efficiently utilized for compression performance prediction (error ratio: 7.45%). Through the biomechanical simulation and theoretical calculations, the tissue stiffness characteristics of applied bodies showed significant correlations (p < 0.05) with the body circumferential displacements but no correlations (p > 0.05) with pressure delivery differences of CTs. This study facilitates the pressure fit design principle and leg mannequin material selection guidance for the development and experimental assessment of CTs. It also provides effective simulation methods for pressure prediction and property parametric optimization of compression materials.

Development and validation of the low-cost pregnant female physical phantom for fetal dosimetry in MV photon radiotherapy.

Monte Carlo (MC) simulations or measurements in anthropomorphic phantoms are recommended for estimating fetal dose in pregnant patients in radiotherapy. Among the many existing phantoms, there is no commercially available physical phantom representing the entire pregnant woman. In this study, the development of a low-cost, physical pregnant female phantom was demonstrated using commercially available materials. This phantom is based on the previously published computational phantom. Three tissue substitution materials (soft tissue, lung and bone tissue substitution) were developed. To verify Tena's substitution tissue materials, their radiation properties were assessed and compared to ICRP and ICRU materials using MC simulations in MV radiotherapy beams. Validation of the physical phantom was performed by comparing fetal doses obtained by measurements in the phantom with fetal doses obtained by MC simulations in computational phantom, during an MV photon breast radiotherapy treatment. Materials used for building Tena phantom are matched to ICRU materials using physical density, radiation absorption properties and effective atomic number. MC simulations showed that percentage depth doses of Tena and ICRU material comply within 5% for soft and lung tissue, up to 25cm depth. In the bone tissue, the discrepancy is higher, but again within 5% up to the depth of 5cm. When the phantom was used for fetal dose measurements in MV photon breast radiotherapy, measured fetal doses complied with fetal doses calculated using MC simulation within 15%. Physical anthropomorphic phantom of pregnant patient can be manufactured using commercial materials and with low expenses. The files needed for 3D printing are now freely available. This enables further studies and comparison of numerical and physical experiments in diagnostic radiology or radiotherapy.

Local Defense Factors in Cleft-Affected Palate in Children before and during Milk Dentition Age: A Pilot Study.

One of the most frequent congenital orofacial defects is the cleft lip and palate. Local tissue defense factors are known to be important in immune response and inflammatory and healing processes in the cleft tissue; however, they have only been researched in older children during mixed dentition. Thus, the aim of this study is to assess the distribution of LL-37, CD-163, IL-10, HBD-2, HBD-3, and HBD-4 in children before and during milk dentition. The unique and rare material of palate tissue was obtained from 13 patients during veloplastic surgeries during the time span of 20 years. Immunohistochemistry, light microscopy, semi-quantitative evaluation, and non-parametric statistical analysis were used. A significant decrease in HBD-3 and HBD-4 in the connective tissue was found, as well as several mutual statistically significant and strong correlations between HBD-2, HBD-3, HBD-4, and LL-37. Deficiency of HBD-3 and HBD-4 suggests promotion of chronic inflammation. The scarcity of HBD-4 could be connected to the different signaling pathways of dental pulp cells. Mutual correlations imply changes in the epithelial barrier, amplified healing efficiency, and increased antibacterial line of defense. Deprivation of changes in IL-10 quantity points to possible suppression of the factor. The presence of similar CD-163 immunoreactive substances produced by M2 macrophages was also observed.

Highly Reinforced Acrylic Resins for Hard Tissue Engineering and Their Suitability to Be Additively Manufactured through Nozzle-Based Photo-Printing.

Mineralized connective tissues represent the hardest materials of human tissues, and polymer based composite materials are widely used to restore damaged tissues. In particular, light activated resins and composites are generally considered as the most popular choice in the restorative dental practice. The first purpose of this study is to investigate novel highly reinforced light activated particulate dental composites. An innovative additive manufacturing technique, based on the extrusion of particle reinforced photo-polymers, has been recently developed for processing composites with a filler fraction (w/w) only up to 10%. The second purpose of this study is to explore the feasibility of 3D printing highly reinforced composites. A variety of composites based on 2,2-bis(acryloyloxymethyl)butyl acrylate and trimethylolpropane triacrylate reinforced with silica, titanium dioxide, and zirconia nanoparticles were designed and investigated through compression tests. The composite showing the highest mechanical properties was processed through the 3D bioplotter AK12 equipped with the Enfis Uno Air LED Engine. The composite showing the highest stiffness and strength was successfully processed through 3D printing, and a four-layer composite scaffold was realized. Mechanical properties of particulate composites can be tailored by modifying the type and amount of the filler fraction. It is possible to process highly reinforced photopolymerizable composite materials using additive manufacturing technologies consisting of 3D fiber deposition through extrusion in conjunction with photo-polymerization.