Tip Region Research Articles

Isothermal and thermomechanical fatigue crack growth behavior of 316LN stainless steel under load‐ and strain‐controlled modes

AbstractCrack growth tests of 316LN stainless steel under load‐controlled and strain‐controlled thermo‐mechanical fatigue (TMF) loadings at the temperature range of 350°C ~ 550°C were conducted. Moreover, the isothermal fatigue (IF) crack growth tests at the maximum temperature of 550°C were performed for comparison. The differences in IF and TMF crack propagation behavior under different loading conditions were thoroughly discussed, and the underlying mechanisms were revealed by a comprehensive characterization of the deformation state of the crack tip region using digital image correlation (DIC) and electron backscatter diffraction (EBSD) methods. Furthermore, it was found that the EBSD characterization was simply based on a single plane made it difficult to evaluate the complex crack growth behavior. In addition, the limitations of the classical parameter of stress intensity factor under TMF loading were presented.

Tip gap effects on film cooling performance of a transonic turbine blade with a distinctive coolant supply arrangement

Considered are the influences of tip gap on film cooling performance variations near to and along the tip or end of a turbine blade with a squealer rim contained within a linear cascade with five airfoils. The film coolant is supplied by two plenums which are positioned at forward and aft locations within the blade. The aft blowing ratio varies between 0.88 and 1.48, the forward blowing ratio is set to be approximately constant in the vicinity of 2.75 to 3.0, and a value of about 1.3 is utilized for the ratio of coolant density relative to the main flow density. Measured results include linearly-averaged and spatially-resolved variations of adiabatic effectiveness, which are presented both for the top portion of the pressure surface and end surface of the blade with the squealer rim. Local effectiveness variations along the top pressure side of the blade show significant effectiveness values downstream of aft pressure side hole outlet locations mostly because film lift-off downstream of these holes is less substantial than observed downstream of the forward holes. Spatially-resolved effectiveness data for the end of the blade with a squealer rim show that the largest values of effectiveness are evident near to and immediately downstream of dusting hole outlet locations. Associated linearly-averaged effectiveness data for the tip of the blade recess region with the 1.2 mm tip gap are as much as about two times higher than values for the 2.0 mm tip gap when compared at each surface location. Local and linearly-averaged surface effectiveness variations for the downstream edge region of the blade squealer tip are most strongly influenced by the film cooling holes which are aft and by the dusting hole which is positioned along the tip of the blade rim near the downstream edge. Here, effectiveness magnitudes are also often higher along the downstream edge rim region with the 1.2 mm tip gap, compared to data associated with the 2.0 mm tip gap.

Implementation of delayed intervention in open approach rhinoplasty for patients with supratip scarring

The columellar incision and dissection which were integral steps in open rhinoplasty play a crucial role in achieving optimal results. However, scarring in the supratip region may pose challenges to skin circulation, potentially leading to complications such as flap loss. This study aims to establish the safety and efficacy of the open rhinoplasty technique as a viable alternative for patients with substantial scars in the supratip region typically addressed using the closed rhinoplasty technique. Patients with scars on the tip or supratip region were operated clinic between February 2010 and March 2023. To mitigate the risk of tip necrosis, a meticulous two-step delay approach was employed. In the initial outpatient procedure, performed under local anesthesia, we opted for a columellar incision. Subsequently, the columellar arteries were identified and cauterized to facilitate the delay procedure. Following a ten-day interval, all patients underwent standard open rhinoplasty. The columellar artery delay procedure proved instrumental in executing successful open rhinoplasty without compromising skin circulation between the scarred supratip and columellar incision line. Our findings suggest that the columellar delay procedure effectively prevents flap loss in open rhinoplasty patients with scars in the supratip and columellar regions. This brief outpatient intervention, lasting approximately ten minutes, provides a reliable method for adopting the open approach in primary rhinoplasty cases, irrespective of prior scarring. Level IV, therapeutic study.

Effect of Tip Gap Size on the Tip Flow Structure and Turbulence Generation in a Low Reynolds Number Compressor Cascade

Abstract Efficient and compact axial compressors are currently undergoing rapid development for use in microcooling systems and small-scale vehicles. Limited experimental work concentrates on the inner flow field of the compressors working at such low Reynolds numbers (Re∼104). This study examines the vortical structures and the resulting turbulence production in the transitional flow over a C4 compressor blade at a Reynolds number Re of 24,000, with a specific focus on the impact of tip clearance. The particle image velocimetry measurements reveal the tip flow structures in detail, including the tip leakage vortex (TLV) and its induced complex vortical structures. The tip secondary flow at the low Reynolds number can be divided as the tip leakage flow (TLF)/vortex and transitional boundary layer both at the end walls and the blade surfaces. The TLV propagates at the highest spanwise positions and farthest pitchwise positions at the middle tip gap size (τ/C = 3%) for the three tip gap sizes investigated. The tip flow fluctuations decrease from τ/C = 5% to τ/C = 3% and then increase from τ/C = 3% to τ/C = 1%. The spatial distribution, streamwise evolution, and individual Reynolds normal stress components contributing to the turbulent kinetic energy (TKE) are discussed. The primary contributors to the turbulence generation are examined to elucidate the flow mechanism leading to the distinct anisotropic turbulence structure in the tip region with various tip gap sizes.

Bleed air CFD modelling in aerodynamic simulation of A heavy duty gas turbine compressor

This study investigates the effects of different bleed air CFD modelling methods on the aerodynamic simulation of a heavy-duty gas turbine multistage compressor with bleed airflow. Two methods, the local source term method and the simplified bleed off-take geometry method, are compared. The results reveal that while overall compressor performance shows minimal variation between the methods, significant differences are observed near the blade tip region. The bleed source term method predicts a more uniform flow field in the bleed cavity, leading to a larger surge margin for the compressor. Therefore, in the analysis of multistage compressors, it is advisable to adopt simplified geometric modeling method for simulating bleed air extraction. Moreover, when utilizing this approach, careful consideration should be given to the choice of the slot inclination angle. This design parameter presents a crucial trade-off between losses within the exhaust structure and those arising from the entry of mainstream flow into the exhaust slot cavity.

Tip-leakage-flow excited unsteadiness and associated control

Tip leakage flow in turbomachinery inherently generates intense unsteady features, named self-excited unsteadiness, which significantly affects the operating stability, aerodynamic efficiency, and noise but has not been well understood. A zonalized large eddy simulation is employed for a linear cascade, with wall-modeled large eddy simulation active only in the tip region. The simulation is well validated with advantages demonstrated for effectively reducing the computational cost while maintaining an equivalent prediction accuracy in the region of interest. The time-averaged and spatial-spectral characteristics of tip leakage vortex (TLV) structures are systematically discussed. The self-excited unsteady processes of TLV include the tip gap separation, the tip leakage and jet-mainstream interaction, the primary tip leakage vortex (PTLV) wandering motion, and the induced separation near end wall. The Spectral Proper Orthogonal Decomposition (SPOD) is used to examine the dominant frequencies and their coherent structures. It is found that these unsteady features change from a single high frequency to multiple lower frequencies due to the PTLV breakdown. The SPOD and correlation analyses reveal that the self-excited unsteadiness originates initially from unsteady vortex separation in the tip gap and is then fed by the interactions between the tip leakage jet and mainstream. The associated unsteady fluctuations are convected along the tip leakage jet trajectory, causing the wandering motion of PTLV core. Based on the revealed unsteadiness sources, a micro-offset tip design is proposed and shown to be an effective solution to reducing the tip flow unsteadiness. This work improves the understanding of tip-leakage-flow dynamics and informs the control of the associated unsteady fluid oscillation and noise.

The puncture mechanics: an example from the bed bug Cimex lectularius showing traumatic insemination using the paramere.

Cimicidae are well-known for traumatic insemination, and males pierce females with their parameres and transfer sperm through them. The shape of parameres is relatively stable in the family, but in some genera, the paramere is elongated, appearing less resistant against lateral deflection. To understand the mechanical limitations of the paramere, we studied its penetration mechanics of the common bed bug, Cimex lectularius. We examined the post-abdominal morphology, paramere geometry and material properties and conducted breaking stress experiments on the paramere under wet and dry conditions. Mechanical property gradients are present with the paramere tip as the stiffest region and the base as the most flexible one. These mechanical properties relate to the presence of Ca, Zn and Si. The basal wing-shaped structure is flexible, enabling it to interlock with the anal region during mating. The paramere is slightly twisted; the tip region is circular in cross-section, and the geometry of the rest is rather complex. In the mechanical tests, wet parameres mainly buckled, while dried parameres broke off. The level of structural failures depended on directions from which the compression forces were applied. Structural, material and mechanical strengthening mechanisms preventing the paramere from mechanical failure are discussed.

Molecular dynamics study on the effect of hydrogen on the plastic behavior of α-Fe crack tip

In this paper, the unilateral tension of α-Fe in the local region of the crack tip are simulated by molecular dynamics method, the plastic deformation behaviors and hydrogen embrittlement mechanism of the crack tip under different hydrogen concentration and hydrogen positions are analyzed. The results show that for the hydrogen-containing models, there exists a critical hydrogen concentration c0 that can make the plasticity of the α-Fe material reduce greatly. For the crack-free model, the main hydrogen embrittlement mechanism is adsorption-induced dislocation emission (AIDE), and the critical hydrogen concentration c0 is 0.3%. When the hydrogen concentration cH<c0, the plastic deformation mechanism is mainly dominated by phase transformation; when cH≥c0, the plastic deformation mechanism is phase transformation at low strains and dislocation motion at high strains. For the cracked model, the main mechanism of hydrogen embrittlement is hydrogen-enhanced local plasticity at the crack tip, with a critical c0 of 1.7%. When cH<c0, the plastic deformation mechanism of the model is a combination of phase transformation and dislocation; when cH≥c0, the plastic deformation mechanism is controlled by phase transformation. When the hydrogen is far away from the crack tip, the local mechanical behavior of the crack tip does not change significantly, while when hydrogen gathered at the crack tip, the addition of a small amount of hydrogen atoms will make the fracture strain decrease rapidly, which shows a mechanism of hydrogen-enhanced strain-induced vacancies (HESIV). A saturation value of hydrogen concentration at the crack tip is confirmed in this paper, when the local hydrogen concentration exceeds this saturation value, the plastic behaviour tends to be stable. The influence of hydrogen atoms distribution on the crack initiation behavior was determined by atomic configuration analysis.

Impact of blade shape on the aerodynamic performance and turbulent jet dynamics produced by a ducted rotor

A ducted rotor system was used to produce turbulent jets with a Reynolds number up to 5.97 × 105 and Mach number of 0.222 based on mean streamwise velocity. Three rotors with a diameter of 11.8 cm were manufactured and tested inside a duct with a 1 mm tip clearance at a speed up to 30 000 revolutions per minute (rpm). All rotor blades contain the same aspect ratio of 2.2, a National Advisory Committee for Aeronautics 2410 airfoil, and ideal pitch distribution. However, three different blade planform shapes were used including a rectangular shape with constant chord, trapezoidal shape with a taper ratio of 0.5, and elliptical shape where the trailing edge of the blade is expressed with an elliptical function. The rotor thrust and electric power were measured, and the thrust coefficient and figure of merit was computed. The flow-field produced by the ducted rotors was measured in the near-field using laser Doppler velocimetry techniques. The inflow velocity approximately 3 mm upstream of the rotor blade leading edge was acquired and its significance on blade aerodynamics and performance is analyzed. Time-averaged contours of cross-stream vorticity reveal intense hub and blade tip vortex structures, which are impacted by the shape of the blade, particularly in the blade tip region. Tip vorticity as well as streamwise turbulence intensity and turbulent kinetic energy in this region were mitigated for the rotors with trapezoidal and elliptical blades. However, the turbulent structure of the jet produced by all three rotor blade shapes showed similarity at a mere 2.8 rotor diameters downstream of the rotor. This finding emphasizes the importance of blade design on the near-field dynamics of ducted rotor flows for aircraft propulsion.

Surface-enhanced optical absorption and induced heating in tapered silicon nanoprobes

Subject of study. This study investigates the correlation between the heating temperature and the mesoscopic shape of the Si probe of an atomic force microscope subjected to medium-intensity laser irradiation (5mW/cm2) in the presence of a rough metal substrate. Aim of study. This study aims to quantitatively assess the dependence of optical-field enhancement and induced heating of the tip of a tapered Si nanoprobe, under laser irradiation, on the radius of curvature and cone angle of the probe tip, the distance between the tip and substrate, and the surface roughness of an Au substrate. Method. The localization of the electromagnetic field in the gap between a Si nanoantenna and a defect on the surface of an Au substrate was simulated using the finite-difference time-domain method. A thin Au coating (thickness up to 50 nm) on a glass substrate was used as a plasmonic surface. Owing to the excitation of surface plasmon resonance, such a coating enhances the absorption of light and increases the heating temperature of the Si optical antenna. Main results. The influence of the polarization angle of the incident laser radiation on the distribution of the electric field near the tip of the probe was examined. Only the component of the incident light field along the direction of the probe axis was enhanced near the tip of the Si cantilever. The influence of various parameters, including the radius of curvature, cone angle of the tip of the Si nanoantenna, distance between the probe and substrate, and surface roughness of the Au substrate, on the maximum temperature in the tip region of the Si probe was investigated. The probe temperature was found to decrease with decreasing cone angle of the probe, whereas the temperature of the cantilever tip decreased as the cone angle of the probe tip increased. The dependence of temperature on the radius of curvature of the Si nanoantenna tip in the presence of an Au substrate was evaluated. With an increase in the surface roughness of the Au film, the temperature of the Si antenna tip increased, gradually approaching a limit value. Practical significance. The results of this study are useful for selecting the optimal parameters of an experiment in which a heated probe is used. Controlled heating of a Si probe can facilitate the study of phase transitions in various nanomaterials and that of local thermochemical nanocatalysis for creating new structural materials with specific properties.

Investigating the influence of various three-point bending specimen configurations on rock fracture parameters testing: an experimental study

The fracture parameters of rocks, including fracture toughness and fracture energy, represent their resistance to crack propagation under external stress. These parameters play a critical role in engineering design, construction safety, geological mechanics research, and rock material assessment. This study determined the fracture parameters of granite under Mode I loading conditions using notched semicircular bend (NSCB) and single-edge notched beam (SENB) specimens. Although there was little difference between the initial and unstable fracture toughness values of the two specimen configurations, significant differences were observed in the fracture process and other fracture parameters. For example, the NSCB specimen exhibits a higher elastic modulus, and it witnessed rapid failure after reaching the peak load, whereas the SENB specimen displayed a more gradual failure process with a distinct post-peak stage. This may be one of the reasons for the higher fracture energy in the NSCB specimen than in the SENB specimen. Based on finite element analysis and the extraction of the tangential stress at the crack tip region, the application of the maximum tangential stress fracture criterion provided a robust explanation for the coherence observed in the unstable fracture toughness test results between the two specimen configurations.

Diagnosis of unsteady disturbance characteristics induced by the tip leakage vortex in a compressor based on data-driven modal decomposition methods

The structural information about the tip leakage vortex at the design point remains largely unknown. Here, the dynamic mode decomposition method is utilized to visualize the main coherent structures corresponding to unsteady disturbance frequencies induced by the tip leakage vortex of an isolated rotor at the design point. The results show that the tip clearance size has a significant impact on unsteady disturbance characteristics at the blade tip region. The flow field within the blade tip region can be categorized into four distinct regions: the formation region of the main tip leakage vortex (MTLV), the formation region of the secondary tip leakage vortex (STLV), the merging zone where the MLTV and the STLV interact, and the vortex shedding zone induced by the leakage vortex breakdown. The disturbance peak in the frequency domain decreases from 121.3 RF to 70.96 RF as the tip clearance size increases from 1.5% blade height to 2%, resulting in a reduction of 41.36%. The increase in the tip clearance size amplifies unsteady disturbances caused by the MTLV and STLV. The STLV exhibits more pronounced oscillatory characteristics than the MTLV. The unsteady disturbance induced by the MLTV mainly occurs at around 0.5 blade passing frequency (BPF). In contrast, high-frequency unsteady disturbances (&amp;gt;1 BPF) in the flow field are caused by vortex shedding resulting from the interaction and collision between the STLV and the MTLV. A better understanding of the unsteady disturbance characteristics induced by leakage vortex benefits the study of stall warning technology.

Automating the determination of low-temperature fracture resistance curves of normal and rubberized asphalt concrete in single-edge notched beam tests using convolutional neural networks

As materials undergo large-scale yielding or exhibit large sizes of fracture process zone in the crack tip region, multi-parameter fracture concepts should be employed to describe the complex crack-tip stress-strain fields. Fracture resistance curves (R-curves) are an established tool in characterizing the entire fracture process of such materials. However, for complex materials such as bituminous mixtures, the development of these curves is subject to experimental and computational intricacies. In this research, a framework is developed to automate the construction of R-curves for normal and rubberized asphalt concrete (AC) mixtures. AC mixtures are produced using PG58–22 and PG58–28 binders. Limestone and siliceous aggregates are used, and three binder contents are considered for the mixtures. Single-edge notched beam (SE(B)) fracture testing is conducted on AC beams with two different notch patterns. A convolutional neural network (CNN) model is developed and trained over 1260 test images with varying temperatures, notch geometries, and setups. The CNN model was used to detect the growing crack on the beam surface and each crack-detected image was sent to an image processing framework to measure the crack length. Crack extension increments were calculated and synchronized with test time and magnitude of load, load-line displacement, and cumulative fracture energy, and the R-curve could be constructed. A training accuracy of 0.91 was obtained for the model and a loss of below 0.10 as a result of a hyperparameter tuning indicating reliable classifications by the CNN architecture. The R-curves showed desirable agreement for control mixtures at temperatures of 0 °C and −15 °C. As the mixtures are rubberized, the R-curves showed favorable agreement in the crack blunting phase, transition zone, as well as the unstable propagation phase at −20 °C. Cohesive energy magnitudes were compared for the two methods with a Pearson coefficient of 0.81 while fracture rate and fracture energy magnitudes showed favorably close magnitudes with coefficients of 0.89 and 0.98 respectively.

A shock loss reduction method for the low-pressure rotor of a vaneless counter-rotating turbine using circulation redistribution

This paper proposes the circulation redistribution for the low-pressure (LP) rotor of a highly-loaded vaneless counter-rotating turbine (VCRT) to reduce the LP shock losses. The method is to decrease the mid-span circulation and increase the tip circulation at the exit of the LP rotor with unchanged high-pressure (HP) and LP power output. The LP Blade-to-Blade flow field can be considered as a Laval nozzle model. And the high static pressure drop from the throat to the exit results in strong trailing edge (TE) shocks near the LP mid-span. Also, the suction side TE shock (ST) and the reflected shock of the pressure side TE shock (RPT) near the LP mid-span merge into a relatively strong shock very close to the TE. These two factors result in high shock losses. To reduce the shock losses under the design condition, decreasing the stagger angle and the blade exit angle near the LP mid-span are adopted to reduce the LP mid-span exit circulation. This changes the throat area to exit area ratio near the LP mid-span, prompting the actual static pressure drop from the throat to the exit to be reduced and closer to the theoretical value, so that the TE shock strength is reduced. Besides, the intersection of the RPT and the ST is delayed owing to the improved flow angle distributions near the TE, further contributing to the LP mid-span shock loss reduction. Since the LP power output is decreased and the LP tip-leakage flow (TLF) losses are far lower than the HP TLF losses, increasing the stagger angle and the blade exit angle in LP tip regions are employed to increase the LP exit circulation and decrease the HP exit circulation in tip regions. Then the HP and LP efficiency are both raised with unchanged HP and LP power output after optimizing the blade profiles near the mid-span and in tip regions of the LP rotor. The remarkable thing is that the TE shock strength in LP tip and root regions are both reduced after improved because of the decreased ratio of the exit area to the throat area. And the intersection of the RPT and the ST in LP tip and root regions are also both delayed, leading to reduced shock losses. The reduced TE shock losses along the whole LP span appreciably raise the LP and VCRT efficiency by 0.96% and 0.32% respectively.

Smart Hydrogel Swelling State Detection Based on a Power-Transfer Transduction Principle.

Stimulus-responsive (smart) hydrogels are a promising sensing material for biomedical contexts due to their reversible swelling change in response to target analytes. The design of application-specific sensors that utilize this behavior requires the development of suitable transduction concepts. The presented study investigates a power-transfer-based readout approach that is sensitive to small volumetric changes of the smart hydrogel. The concept employs two thin film polyimide substrates with embedded conductive strip lines, which are shielded from each other except at the tip region, where the smart hydrogel is sandwiched in between. The hydrogel's volume change in response to a target analyte alters the distance and orientation of the thin films, affecting the amount of transferred power between the two transducer parts and, consequently, the measured sensor output voltage. With proper calibration, the output signal can be used to determine the swelling change of the hydrogel and, consequently, to quantify the stimulus. In proof-of-principle experiments with glucose- and pH-sensitive smart hydrogels, high sensitivity to small analyte concentration changes was found along with very good reproducibility and stability. The concept was tested with two exemplary hydrogels, but the transduction principle in general is independent of the specific hydrogel material, as long as it exhibits a stimulus-dependent volume change. The application vision of the presented research is to integrate in situ blood analyte monitoring capabilities into standard (micro)catheters. The developed sensor is designed to fit into a catheter without obstructing its normal use and, therefore, offers great potential for providing a universally applicable transducer platform for smart catheter-based sensing.

Fracture ellipticity as a measure of chemical reaction-controlled fracture growth

Fracture ellipticity as a measure of chemical reaction-controlled fracture growth

Rotating Flow and Heat Transfer Characteristics of a Novel Cooling Channel for Gas Turbine Blade Trailing Edge With Diamond-Type TPMS Structures

Abstract This work designs a Diamond-type triply periodic minimal surface (TPMS) structure that exhibits excellent thermomechanical properties in a gas turbine blade trailing edge to enhance thermal performance and improve heat transfer uniformity. Since the velocity and temperature distributions are altered in the rotating trailing edge channel, the flow and heat transfer characteristics of the baseline pin fin and Diamond TPMS models are numerically investigated at the Reynolds number of 10,000 and the rotation numbers of 0.0–0.28. Compared to the baseline model, the Diamond TPMS network significantly decreases recirculation flow at the inner wall, improving heat transfer, especially at the tip and outlet regions. Although the Diamond TPMS model incurs substantial pressure losses from 191% to 234%, it yields significantly higher overall heat transfer than the pin fins by 179%. Consequently, the thermal performance increased by 93.4%. The flow fluctuations due to the rotating effects are minor in the Diamond TPMS architecture, considerably reducing the differences in heat transfer between the leading and trailing walls. The differences in the wetted-area averaged Nusselt number of the baseline and Diamond TPMS models within the studied rotation numbers are 8.5–14.4% and about 8.5%, respectively. Moreover, the Diamond TPMS structure reduces the differences in heat transfer between the root and tip regions at the outlet by up to 80% compared to the pin fins. This improvement helps protect the trailing edge from thermal failure, thereby potentially prolonging the gas turbine blade's lifetime.

Janus electronic state of supported iridium nanoclusters for sustainable alkaline water electrolysis

Metal-support electronic interactions play crucial roles in triggering the hydrogen spillover (HSo) to boost hydrogen evolution reaction (HER). It requires the supported metal of electron-rich state to facilitate the proton adsorption/spillover. However, this electron-rich metal state contradicts the traditional metal→support electron transfer protocol and is not compatible with the electron-donating oxygen evolution reaction (OER), especially in proton-poor alkaline conditions. Here we profile an Ir/NiPS3 support structure to study the Ir electronic states and performances in HSo/OER-integrated alkaline water electrolysis. The supported Ir is evidenced with Janus electron-rich and electron-poor states at the tip and interface regions to respectively facilitate the HSo and OER processes. Resultantly, the water electrolysis (WE) is efficiently implemented with 1.51 V at 10 mA cm–2 for 1000 h in 1 M KOH and 1.44 V in urea-KOH electrolyte. This research clarifies the Janus electronic state as fundamental in rationalizing efficient metal-support WE catalysts.

Induction and characterization of induced tetraploid, mixoploid and control diploid teak seedlings (Tectona grandis L.f.) from shoot cultures treated with oryzalin*

Teak, known for its high-quality timber and economic value, holds significant potential as the major component of an agroforestry system to combat climate change and support sustainable development goals. The challenge of slow teak growth can be addressed by introducing fast-growing teak varieties amenable to vegetative propagation. Furthermore, the improvement of teak through somatic cell genetics, such as inducing polyploidy, can result in more vigorous, faster-growing, and drought-tolerant trees. While a report exists on a tetraploid teak accession with enhanced less water tolerance, information on the induction and characterization of tetraploid teak is lacking. In this study, we report the induction of polyploid teak by immersing 1 – 1.5 cm nodal stem segments and shoot tips obtained from teak shoot cultures in a modified MS liquid medium containing 5 − 30 µM oryzalin for one week, followed by subculture for 6 passages. The resulting shoots were acclimatized and grown in the nursery for 10 months. Based on flow cytometry for ploidy analysis of pre-selected plants having morphologies typical of polyploid followed by chromosome counting, we identified two tetraploid plants from one accession and 18 mixoploid teak plants from four accessions. Chromosome counting analysis confirmed that a diploid plant had a chromosome number of 2n = 2x = 36, tetraploid plant had a chromosome number of 2n = 4x = 72, and mixoploid plant exhibited both chromosome number of 2n = 2x = 36 and 2n = 4x = 72 in root tip cells. Morphological characterization revealed that tetraploid and mixoploid teak plants displayed typical polyploid characteristics, such as larger, thicker, and greener leaves, increased root diameter, increased cell size in root tip region, and larger stomata size but with reduced density. The development of tetraploid teak holds the potential to provide faster-growing and more drought-tolerant varieties, facilitating breeding efforts, including the development of triploid varieties.

Complicated flow in tip flow field of a compressor tandem cascade using delayed detached eddy simulation

The complex flow characteristics in the tip region of a tandem cascade with tip clearance have been calculated and analyzed using Delayed Detached Eddy Simulation (DDES). The coherent mechanism of the vortex structures near the blade tip was discussed, and the unsteady behaviors and features in the tip flow field were analyzed. Additionally, the interaction between the tip leakage flow and the gap jet was revealed. The results show that, compared to the datum cascade, the blade tip load of the rear blade increases while that of the front blade decreases. Unsteady fluctuations of the tandem cascade are mainly caused by the interaction between the tip leakage flow and gap jet, and by the mixing of the vortex structures, but there is no essential change in the spectrum feature of the tip leakage flow. Finally, a detailed analysis of the development of vortices in the tip region is conducted by the topological structures of the flow field. Combined with the three-dimensional vortex structures, the schematic diagram of the vortex system of the datum single-row cascade and tandem cascade is summarized.