Time Cues Research Articles

Influence of Actor's Congruent and Incongruent Gaze on Language Processing.

In interpreting spoken sentences in event contexts, comprehenders both integrate their current interpretation of language with the recent past (e.g., events they have witnessed) and develop expectations about future event possibilities. Tense cues can disambiguate this linking but temporary ambiguity in their interpretation may lead comprehenders to also rely on further, situation-specific cues (e.g., an actor's gaze as a cue to his future actions). How comprehenders reconcile these different cues in real time is an open issue that we must address to accommodate comprehension. It has been suggested that relating a referential expression (e.g., a verb) to a referent (e.g., a recent event) is preferred over relying on other cues that refer to the future and are not yet referentially grounded (“recent-event preference”). Two visual-world eye-tracking experiments compared this recent-event preference with effects of an actor's gaze and of tense/temporal adverbs as cues to a future action event. The results revealed that people overall preferred to focus on the recent (vs. future) event target in their interpretation, suggesting that while a congruent and incongruent actor gaze can jointly with futuric linguistic cues neutralize the recent-event preference late in the sentence, the latter still plays a key role in shaping participants' initial verb-based event interpretation. Additional post-experimental memory tests provided insight into the longevity of the gaze effects.

P049 Sleep Regularity is Associated with Stability of Daily Light Exposure in Adolescents During School and Vacation

Abstract Study Objectives Light is the main time cue for the human circadian system. Irregular sleep/wake patterns are associated with poor health outcomes, which could be mediated by irregular patterns of light exposure. The relationship between sleep and light regularity has not been directly explored. We investigated the relationship between sleep and light regularity in adolescents, across school-term and vacation, using novel metrics for measuring light regularity. Methods Daily sleep and light patterns were measured via wrist actigraphy in 104 adolescents (54% male, age M±SD = 17.17±0.80 years) over two weeks of school-term and a subsequent two-week vacation. The Sleep Regularity Index (SRI) was computed for each two-week block. Stability of daily light exposure was assessed using variation of mean daily light timing (MLiT), variation in daily photoperiod, and the Light Regularity Index. Associations between SRI and each light regularity metric were examined, and within-individual changes in metrics were examined between school and vacation. Results More regular sleep was significantly associated with more regular scores for each light variability metric, during school and vacation. Between school and vacation sleep regularity decreased and nuanced changes in light patterns were observed. Variability measured by the MLiT variable increased, whereas variability measured by the LRI and photoperiod variable decreased. Conclusions Adolescents with irregular sleep also have irregular patterns of light exposure. These findings suggest sleep regularity may be a useful proxy for variability in the main circadian time cue, meaning that irregular light exposure may carry implications for the developing adolescent circadian system.

The role of marbling as an intrinsic characteristic at the point of meat purchase–the Taguchi approach

Meat quality is considered a complex concept depending on many characteristics that could be intrinsic or extrinsic. At the same time, intrinsic and extrinsic quality cues affect consumers’ purchasing decisions. The importance of each quality cue was analysed and discussed in previous literature. Thus, colour and level of marbling of fresh meat were defined as key quality cues at the point of meat purchase. These characteristics are mostly related to pork and beef. The aim of this study was to identify quality characteristics that most closely match the consumer’s preferences and at the same time could be related to quality losses. For that purpose, this paper gives a novel approach of the potential application of Taguchi loss function associated with quality characteristics and related losses for colour and level of marbling. This application can be implemented by providing a quality characteristic’s proper target values and limits, which would make the meat production process more consistent.

Web-based remote testing as a viable option for measuring binaural hearing abilities

Web-based remote testing can increase access to clinically diverse populations in hearing research. However, whether remote testing affects data quality compared to in-lab testing is unknown. Data for binaural hearing tasks may be particularly vulnerable to commercial-grade hardware limitations and background noise from the testing environment. We replicated two published studies on binaural hearing abilities using a web-based behavioral experimental platform (Gorilla.sc). In Experiment 1, we replicated an experiment by Goupell et al. [JASA 133(4) (2013)] that tested intra-cranial lateralization to interaural time and level difference cues. In Experiment 2, we replicated an experiment by Goupell et al. [JASA 140(3) (2016)] that investigated binaural and contralateral unmasking of speech. Two groups of college-aged young adults were tested: one with verified normal hearing (NH) and one with self-reported NH. Testing took place in participants’ homes using their own computer and audio hardware. Performance was compared between the two groups of participants as well as with published data collected in-lab. For both experiments, performance was similar for in-lab and web-based remote testing. We found no effect of verified versus self-reported NH. These results suggest that remote testing may be a feasible option for binaural hearing experiments. [Work funded by NIH-NIDCD.]

Comment on “COVID-19, the Built Environment, and Health”

Comment on “COVID-19, the Built Environment, and Health”

Behaviorally and environmentally induced non-24-hour sleep-wake rhythm disorder in sighted patients.

To determine whether there was evidence of circadian or sleep-regulatory dysfunction in sighted individuals with non-24-hour sleep-wake rhythm disorder. Three sighted individuals with signs and/or symptoms of non-24-hour sleep-wake rhythm disorder were studied. Thirty-five- to 332-day laboratory and home-based assessments of sleep-wake and circadian timing, endogenous circadian period, photic input to the circadian pacemaker, and/or circadian and sleep-wake-dependent regulation of sleep were conducted. No evidence of circadian dysfunction was found in these individuals. Instead, sleep-wake timing appeared to dissociate from the circadian timing system, and/or self-selected sleep-wake and associated light/dark timing shifted the circadian pacemaker later, rather than the circadian pacemaker determining sleep-wake timing. These findings suggest that the etiology of this disorder may be light- and/or behaviorally induced in some sighted people, which has implications for the successful treatment of this disorder. Emens JS, St Hilaire MA, Klerman EB, etal. Behaviorally and environmentally induced non-24-hour sleep-wake rhythm disorder in sighted patients. J Clin Sleep Med. 2022;18(2):453-459.

Pitch perception is more robust to interference and better resolved when provided by pulse rate than by modulation frequency of cochlear implant stimulation

Cochlear implants are medical devices that have been used to restore hearing to more than half a million people worldwide. Most recipients achieve high levels of speech comprehension through these devices, but speech comprehension in background noise and music appreciation in general are markedly poor compared to normal hearing. A key aspect of hearing that is notably diminished in cochlear implant outcomes is the sense of pitch provided by these devices. Pitch perception is an important factor affecting speech comprehension in background noise and is critical for music perception. The present article summarizes two experiments that examine the robustness and resolution of pitch perception as provided by cochlear implant stimulation timing. The driving hypothesis is that pitch conveyed by stimulation timing cues is more robust and better resolved when provided by variable pulse rates than by modulation frequency of constant-rate stimulation. Experiment 1 examines the robustness for hearing a large, one-octave, pitch difference in the presence of interfering electrical stimulation. With robustness to interference characterized for an otherwise easily discernible pitch difference, Experiment 2 examines the resolution of discrimination thresholds in the presence of interference as conveyed by modulation frequency or by pulse rate. These experiments test for an advantage of stimulation with precise temporal cues. The results indicate that pitch provided by pulse rate is both more robust to interference and is better resolved compared to when provided by modulation frequency. These results should inform the development of new sound processing strategies for cochlear implants designed to encode fundamental frequency of sounds into precise temporal stimulation.

Adult perception of stop consonant voicing in American-English-learning toddlers: Voice onset time and secondary cues.

Most studies of speech perception employ highly controlled stimuli. It is not always clear how such results extend to the processing of natural speech. In a series of experiments, we progressively explored the role of voice onset time (VOT) and potential secondary cues in adult labeling of stressed syllable-initial /b d p t/ produced by typically developing two-year-old learners of American English. Taken together, the results show the following: (a) Adult listeners show phoneme boundaries in labeling functions comparable to what have been established for adult speech. (b) Adult listeners can be sensitive to distributional properties of the stimulus set, even in a study that employs highly varied naturalistic productions from multiple speakers. (c) Secondary cues are available in the speech of two-year-olds, and these may influence listener judgments. Cues may differ across places of articulation and the VOT continuum. These results can lend insight into how clinicians judge child speech during assessment and also have implications for our understanding of the role of primary and secondary acoustic cues in adult perception of child speech.

Abstract #1002771: Effects of Jet Lag on Hormones: Case Report of the Impact of Circadian Change on Thyrotropin (TSH) and Prolactin (PRL) Secretion

Loss of sleep is an endemic problem-with consequences. In jet lag, desynchronization of the circadian clock is exacerbated by the number of time zones crossed, eastward travel, loss of sleep and availability of local time cues. Factors altering TSH and PRL in a jet lag-affected individual are illustrated here.

Association of morningness–eveningness preference with physical activity during the COVID-19 pandemic social distancing: a cross-sectional survey in Brazil

ABSTRACT Social distancing (SDIST) to contain COVID-19 pandemic spread implies reduced sunlight exposure and social daily life, which delay the circadian system and increase eveningness preference. The regular practice of physical activity (PA) is a time cue that decreased during SDIST. However, it is unknown if decreased PA may be associated with increase of eveningness preference. This study aimed to investigate if PA changes might be associated with changes in the morningness-eveningness preference of individuals practicing SDIST in Brazil. For this, 322 adults (18–89 years-old) regularly living in Brazil between March and October 2020 answered an online survey including questions considering the before and during SDIST period on PA (min/week) and morningness-eveningness questionnaire score. Sociodemographic, SDIST, anthropometric, and health characteristics were also included in the online survey. Participants self-reported an increase of eveningness preference comparing Before-SDIST with During-SDIST scores (56 ± 12 vs. 52 ± 13, p < .0001). Self-reported PA decreased comparing Before-SDIST with During-SDIST (230 ± 170 vs. 149 ± 155 min/week, p < .0001). Decrease in the total volume of PA and hours spent outside per day, and higher body mass index were associated with the increase in eveningness preference (R2 = .077), although the decrease in the total volume of PA was the strongest association (R2 = .037). In summary, our results show that SDIST may cause a delay in the circadian system, which is associated with the decrease of PA, a reduction in the hours spent outside per day with sunlight exposure, and obesity.

A Mouse Model of Shiftwork Reveals Sex-Specific Impairments in Circadian Behavior and Reproductive Tissue Timekeeping

Approximately 21 million Americans are employed in nighttime or rotating shiftwork, which is linked with numerous health risks, including reduced reproductive success. While there is a strong body of clinical evidence associating shift workers with deficits in fertility, very little is known about the mechanisms by which shiftwork produces reproductive dysfunction. One important feature of shiftwork is extensive exposure to light at night. Light is one of the strongest timing cues for the circadian system, where exposure to mistimed light (light at night) can disrupt timekeeping throughout the body, leading to a mistiming of physiological processes and resulting in disease development. Our goal is to determine if a light-based model of rotating shiftwork (shift-light), composed of an alternating 6 hour phase advance or delay every 4 days for 5-10 weeks, disrupts circadian rhythms in the reproductive hypothalamic-pituitary-gonadal (HPG) axis, leading to mis-timed tissue rhythms and reduced fertility. We hypothesize that shift-light disrupts wheel running behavior and underlying cellular circadian rhythms in the HPG axis, resulting in reduced reproductive function. Using the validated circadian Per2:luciferase reporter mice, we assessed wheel-running behavior and Per2:luciferase rhythms in tissue explants from males and females. Behavioral data revealed that both sexes adapt their wheel running to shift-light paradigm; however, females, but not males, displayed a significant deficit in their ability to entrain to phase advances following 4 shifts. This sex-specific disruption was supported by preliminary tissue explant Per2:luciferase rhythms, which suggest that shift-light alters tissue level circadian phase synchrony in female, but not male, HPG axis tissues. Importantly, females exhibited shortened estrous cycling during shift-light, suggesting the altered HPG axis synchrony could be directly impacting reproductive function. We are currently working to extend this work to determine how this desynchrony impacts hormone release, including luteinizing hormone and follicle stimulating hormone. Together, this work provides insight into how shiftwork may influence circadian rhythms in reproductive tissues and suggests that females may have increased vulnerability to reproductive deficits from shiftwork.

Phonetic feature size in second language acquisition: Examining VOT in voiceless and voiced stops

Featural approaches to second language phonetic acquisition posit that the development of new phonetic norms relies on sub-phonemic features, expressed through a constellation of articulatory gestures and their corresponding acoustic cues, which may be shared across multiple phonemes. Within featural approaches, largely supported by research in speech perception, debate remains as to the fundamental scope or ‘size’ of featural units. The current study examines potential featural relationships between voiceless and voiced stop consonants, as expressed through the voice onset time cue. Native English-speaking learners of Spanish received targeted training on Spanish voiceless stop consonant production through a visual feedback paradigm. Analysis focused on the change in voice onset time, for both voiceless (i.e. trained) and voiced (i.e. non-trained) phonemes, across the pretest, posttest, and delayed posttest. The results demonstrated a significant improvement (i.e. reduction) in voice onset time for voiceless stops, which were subject to the training paradigm. In contrast, there was no significant change in the non-trained voiced stop consonants. These results suggest a limited featural relationship, with independent voice onset time (VOT) cues for voiceless and voices phonemes. Possible underlying mechanisms that limit feature generalization in second language (L2) phonetic production, including gestural considerations and acoustic similarity, are discussed.

Individual Ants Do Not Show Activity-Rest Rhythms in Nest Conditions.

Circadian rhythms, which respond to the day-night cycle on the earth, arise from the endogenous timekeeping system within organisms, called the "biological clock." For accurate circadian rhythms, daily fluctuations in light and temperature are considered one of the important time cues. In social insects, both abiotic and biotic factors (i.e., social interactions) play a significant role in activity-rest rhythm regulation. However, it is challenging to monitor individual activity-rest rhythms in a colony because of the large group size and small body size. Therefore, it is unclear whether individuals in a colony exhibit activity-rest rhythms and how social interactions regulate their activity-rest rhythms in the colony. This study developed an image-based tracking system using 2D barcodes for Diacamma cf. indicum from Japan (a monomorphic ant) and measured the locomotor activities of all colony members under laboratory colony conditions. We also investigated the effect of broods on activity-rest rhythms by removing all broods under colony conditions. Activity-rest rhythms appeared only in isolated ants, not under colony conditions. In addition, workers showed arrhythmic activities after brood removal. These results suggested that a mixture of social interactions, and not light and temperature, induces the loss of activity-rest rhythms. These results contribute to the knowledge of a diverse pattern of circadian activity rhythms in social insects.

Randomised controlled trial of a just-in-time adaptive intervention (JITAI) smoking cessation smartphone app: the Quit Sense feasibility trial protocol

IntroductionA lapse (any smoking) early in a smoking cessation attempt is strongly associated with reduced success. A substantial proportion of lapses are due to urges to smoke triggered by situational...

Circadian rhythm dysfunction in bipolar affective disorder

IntroductionSleep is paramount in bipolar affective disorder and sleep disturbance can be a trigger or initial manifestation of an episode of illness. Changes in the circadian rhythm in bipolar affective disorder have consistently been recognized and reported, however, this feature can be overlooked in daily clinical practice.ObjectivesWe aim to review and summarize the literature regarding changes in circadian rhythm in patients with bipolar affective disorder.Methods We performed an updated review in the PubMed database using the terms “circadian rhythm” and “bipolar affective disorder”.Results Irregularity of the sleep–wake rhythm, eveningness chronotype, abnormality of melatonin secretion, vulnerability of clock genes, and the irregularity of social time cues are circadian rhythm markers disrupted in bipolar affective disorder. Circadian rhythm dysfunction might be a trait marker of this illness and can act as a predictor for the first onset of bipolar affective disorder and the relapse of mood episodes. Achieving normalization of circadian rhythm in combination with pharmacological, psychosocial and chronobiological treatments can be a tool for managing bipolar affective disorder.Conclusions Recognizing patterns of changes in circadian rhythms is important to detect and diagnose bipolar disorder in clinical practice, also affecting treatment. These alterations are often overlooked and can lead to inadequate treatment and management.

The Roles of Linguistic Experience, Direction of Rhythmic Change, and Timing Cues in Discriminating Different Rhythmic Patterns

This research examined the roles of linguistic experience, direction of rhythmic change and timing cue in the discrimination of different rhythmic patterns by native speakers of English, Chinese advanced EFL learners, Korean advanced EFL learners and Chinese beginning EFL learners in an AAX discrimination test. Results showed that the participants were able to discriminate the rhythmic patterns of different utterances, but the discrimination was not driven by their linguistic experience. Rather, their performances were dependent on the rhythmic patterns of the context stimuli (AA stimuli) in the AAX trial. Participants performed better in the trails where the X stimuli were preceded by syllable- timed AA stimuli. Regarding the role of timing cues in rhythm discrimination, the speech rhythm differences made bigger contributions to rhythm discrimination than speech tempo differences. Moreover, the timing cues of vocalic intervals played more important roles than their consonantal counterparts.

Evidence for Co-Evolution of Masking With Circadian Phase in Drosophila Melanogaster.

Heritable variation in the timing of rhythmic events with respect to daily time cues gives rise to chronotypes. Despite its importance, the mechanisms (clock or non-clock) regulating chronotypes remain elusive. Using artificial laboratory selection for divergent phasing of emergence of adults from pupae, our group has derived populations of Drosophila melanogaster which are early and late chronotypes for eclosion rhythm. Several circadian rhythm characteristics of these populations have since been described. We hypothesized that our selection protocol has inadvertently resulted in selection for masking, a non-clock phenomenon, in the early chronotype due to the placement of our selection window (which includes the lights-ON transition). We designed experiments to discriminate between enhanced masking to light versus circadian clock mediated changes in determining enhanced emergence in the morning window in our early chronotypes. Using a series of phase-shift protocols, LD-DD transition, and T-cycle experiments, we find that our early chronotypes have evolved positive masking, and their apparent entrained phases are largely contributed by masking. Through skeleton T-cycle experiments, we find that in addition to the evolution of greater masking, our early chronotypes have also evolved advanced phase of entrainment. Furthermore, our study systematically outlines experimental approaches to examine relative contributions of clock versus non-clock control of an entrained behavior. Although it has previously been suggested that masking may confer an adaptive advantage to organisms, here we provide experimental evidence for the evolution of masking as a means of phasing that can complement clock control of an entrained behavior.

Food Entrainment, Arousal, and Motivation in the Neonatal Rabbit Pup.

In the newborn rabbit, the light entrainable circadian system is immature and once a day nursing provides the primary timing cue for entrainment. In advance of the mother’s arrival, pups display food anticipatory activity (FAA), and metabolic and physiological parameters are synchronized to this daily event. Central structures in the brain are also entrained as indicated by expression of Fos and Per1 proteins, GFAP, a glial marker, and cytochrome oxidase activity. Under fasting conditions, several of these rhythmic parameters persist in the periphery and brain, including rhythms in the olfactory bulb (OB). Here we provide an overview of these physiological and neurobiological changes and focus on three issues, just beginning to be examined in the rabbit. First, we review evidence supporting roles for the organum vasculosum of lamina terminalis (OVLT) and median preoptic nucleus (MnPO) in homeostasis of fluid ingestion and the neural basis of arousal, the latter which also includes the role of the orexigenic system. Second, since FAA in association with the daily visit of the mother is an example of conditioned learning, we review evidence for changes in the corticolimbic system and identified nuclei in the amygdala and extended amygdala as part of the neural substrate responsible for FAA. Third, we review recent evidence supporting the role of oxytocinergic cells of the paraventricular hypothalamic nucleus (PVN) as a link to the autonomic system that underlies physiological events, which occur in preparation for the upcoming next daily meal. We conclude that the rabbit model has contributed to an overall understanding of food entrainment.

Spatial odor discrimination in the hawkmoth, Manduca sexta (L.)

ABSTRACTFlying insects track turbulent odor plumes to find mates, food and egg-laying sites. To maintain contact with the plume, insects are thought to adapt their flight control according to the distribution of odor in the plume using the timing of odor onsets and intervals between odor encounters. Although timing cues are important, few studies have addressed whether insects are capable of deriving spatial information about odor distribution from bilateral comparisons between their antennae in flight. The proboscis extension reflex (PER) associative learning protocol, originally developed to study odor learning in honeybees, was used as a tool to ask if hawkmoths, Manduca sexta, can discriminate between odor stimuli arriving on either antenna. We show moths discriminated the odor arrival side with an accuracy of >70%. Information about spatial distribution of odor stimuli may be available to moths searching for odor sources, opening the possibility that they use both spatial and temporal odor information.This article has an associated First Person interview with the first author of the paper.

Peripheral Sensory Organs Contribute to Temperature Synchronization of the Circadian Clock in Drosophila melanogaster.

Circadian clocks are cell-autonomous endogenous oscillators, generated and maintained by self-sustained 24-h rhythms of clock gene expression. In the fruit fly Drosophila melanogaster, these daily rhythms of gene expression regulate the activity of approximately 150 clock neurons in the fly brain, which are responsible for driving the daily rest/activity cycles of these insects. Despite their endogenous character, circadian clocks communicate with the environment in order to synchronize their self-sustained molecular oscillations and neuronal activity rhythms (internal time) with the daily changes of light and temperature dictated by the Earth’s rotation around its axis (external time). Light and temperature changes are reliable time cues (Zeitgeber) used by many organisms to synchronize their circadian clock to the external time. In Drosophila, both light and temperature fluctuations robustly synchronize the circadian clock in the absence of the other Zeitgeber. The complex mechanisms for synchronization to the daily light–dark cycles are understood with impressive detail. In contrast, our knowledge about how the daily temperature fluctuations synchronize the fly clock is rather limited. Whereas light synchronization relies on peripheral and clock-cell autonomous photoreceptors, temperature input to the clock appears to rely mainly on sensory cells located in the peripheral nervous system of the fly. Recent studies suggest that sensory structures located in body and head appendages are able to detect temperature fluctuations and to signal this information to the brain clock. This review will summarize these studies and their implications about the mechanisms underlying temperature synchronization.