Time Of Formation Research Articles

Assessment of the age and conditions of metamorphism of high-pressure blastolites of the Gonzhinsky block of the Argun superterrane of the eastern part of the Central Asian folded belt

As a result of petrographic and thermobaric studies, 4 mineral associations were identified in the blastolite: relict, main, association corresponding to biotitisation and association of retrograde changes. The relict association probably represented eclogites The main mineral association corresponds to apoeclogite zoisite amphibolites. Determination of the P-T parameters of the formation of this association showed an interval of P = 13.0–15.0 kbar and T = 580-670 °C. At the same time, if we take the most ferruginous outer rim of amphibole-1 for calculations, then the P-T parameters shift to a higher temperature region of P = 13.0–17.0 kbar and T = 660-780 °C. The association corresponding to the imposed biotitisation is well structurally expressed. The lines of monomineral equilibria of the biotite association constructed from various combinations of garnet, biotite and plagioclase compositions showed good convergence in the local region corresponding to the interval P = 12.5-13.2 kbar and T = 810-830 °C. The last association of retrograde changes includes low-alumina amphibole-2.The association corresponding to the imposed biotitisation.ше is well structurally expressed. The last association of retrograde changes includes low-alumina amphibole-2. Based on the obtained isotopic data, a mineral Sm-Nd isochron was constructed for the rock, garnet and two amphiboles. The age of the isochron is 171 ± 3 million years, the latter is interpreted as the time of formation of the main mineral association and corresponds to its crystallization at the peak of metamorphism.

Genesis of Devonian volcanic-associated Lahn-Dill-type iron ores – part II: trace element fractionation evidences diffuse fluid venting

The iron (Fe)-oxide deposits of the Lahn-Dill-type are composed of haematite-quartz and rare siderite-haematite ores. These ores formed as marine chemical sediments on top of volcaniclastic rocks near the Middle to Late Devonian boundary (∼ 380 Ma). As such, their trace element fractionation patterns provide key information on venting style, ocean chemistry, particle-solution interaction, and depositional environment at the time of ore formation. This study combines WDXRF and ICP-MS/OES whole-rock geochemistry with complementary in-situ LA-ICP-MS analysis, and TEM element mapping of ore samples from the Fortuna Mine (Rhenish Massif, Germany). In-situ measurments were conducted on quartz-haematite, haematite, and siderite-haematite microdomains. Bulk major element contents of the ores indicate (volcani)clastic contamination and post-depositional hydrothermal alteration. Microdomain trace element distributions reveal four different trace element signatures, which are related to: (1) syngenetic apatite formation due to sorption of P and REY from seawater; (2) Fe-(oxyhydr)oxide-specific trace element scavenging and fractionation within the seawater column; (3) diagenetic Fe(III) reduction and trace element mobilisation in pore water; and (4) simultaneous deposition of (volcani)clastic material and Fe-(oxyhydr)oxides. These results show that Lahn-Dill-type iron ore formation resulted from mixing of a low-temperature vent fluid with ambient seawater at high seawater to vent fluid ratios. This likely was related to an environment in which diffuse venting dominated over focused venting, and in which quick Fe-particle precipitation led to formation of haematite-quartz ores. Local diagenetic Fe(III) reduction resulted in post-depositional siderite-haematite ore formation during which trace elements were partially remobilised in pore water.

Cyclodextrin grafted chitosan thermosensitive hydrogel using dual gelling agents: Drug delivery and antibacterial materials

In this paper, three kinds of cyclodextrin-grafted-chitosan (CD-g-CS) were synthesized from chitosan (CS) and β-cyclodextrin (β-CD) with different ratios. And a series of hydrogel with good temperature sensitivity and injectability were prepared by using sodium glycerophosphate (GP) and ammonium hydrogen phosphate (AHP) as dual gelling agents. The gelation formation time of hydrogel was short, and the shortest time was 0.75 min. The FTIR spectrum and 1H-NMR spectrum showed the structure of the compounds and intermolecular interactions. The molecular docking technique probed the encapsulation mechanism of the drug with β-CD. The SEM results showed that the hydrogel had a highly interconnected three-dimensional spatial structure. The swelling ratio of hydrogel was not less than 150 % within 1 h and the degradation rate of hydrogel was not less than 75 % within 14 days. The in vitro drug release results showed that the hydrogel had optimal sustained release effect, and the cumulative release ratio was 58.3 % in 34 h. The release kinetics analysis exhibited that the hydrogel conformed to the Riter-Peppas model. The hemocompatibility test and cytotoxicity test proved that the hydrogel had good biocompatibility and biosafety. The antibacterial experiments showed that the hydrogel could inhibit the biological activity of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Therefore, the CD-g-CS/GP-AHP hydrogel has a broad application prospect in the biological fields of drug delivery and antibacterial materials.

Near-infrared photothermal lens as microfluidic analyzer for determination of phosphate in food and biological samples

In this work, photothermal lens spectrophotometer (PTLS) with crossed-beam configuration pumped by a laser diode (850 nm) was used for determination of soluble phosphorus exists mainly in the form of orthophosphate (PO43−) in nonalcoholic beverage and human blood serum samples. The determination was carried out after complexation reaction between blue molybdenum and phosphate in aqueous solution. UV–vis spectrophotometry was used by measuring absorption in the near infrared region (815 nm) for optimizing the complex formation time, concentrations of reducing agent and sulfuric acid. The UV–vis absorption measurements showed that the calibration curve was obtained in the concentration ranges of 0.5–80 mg L−1 with a linear correlation coefficient of 0.98 and a detection limit of 0.2 mg L−1. Then, PTLS at a wavelength of 850 nm after optimizing the device conditions (flow rate, excitation laser power and interrupting frequency) was used for phosphate determination. The calibration curve in the concentration range of 11–1000 µg L−1 has a correlation coefficient of 0.98 and a detection limit of 4 µg L−1. The achieved detection limit was about 50 times better than NIR absorption spectrometry using a conventional 1 cm sample cell. In PTLS with the 3 μL microfluidic cell experimental detectability amount of phosphate is 33 picogram, while in UV–vis-NIR method this amount is 1.5 µg.

Positronium image of the human brain in vivo.

Positronium is abundantly produced within the molecular voids of a patient's body during positron emission tomography (PET). Its properties dynamically respond to the submolecular architecture of the tissue and the partial pressure of oxygen. Current PET systems record only two annihilation photons and cannot provide information about the positronium lifetime. This study presents the in vivo images of positronium lifetime in a human, for a patient with a glioblastoma brain tumor, by using the dedicated Jagiellonian PET system enabling simultaneous detection of annihilation photons and prompt gamma emitted by a radionuclide. The prompt gamma provides information on the time of positronium formation. The photons from positronium annihilation are used to reconstruct the place and time of its decay. In the presented case study, the determined positron and positronium lifetimes in glioblastoma cells are shorter than those in salivary glands and those in healthy brain tissues, indicating that positronium imaging could be used to diagnose disease in vivo.

Investigating the impacts of a recirculation sedimentation application on microalgae biomass cultivation in wastewater treatment

Commercial microalgae production is often interrupted by contamination, leading to short production cycles, reinoculation needs, and culture collapses, significantly increasing costs. This study focuses on investigating Recirculated Sedimentation Application (RSA) to control contamination in microalgae culture systems used for wastewater treatment. Chlorella vulgaris culture was grown in an unsterilized mixture of tertiary treatment effluent and centrate of anaerobic digestion wastewater sludge over a 90-day experimental period. 60 L raceway reactor was operated under a light intensity of 275 μM m−2.s−1 with a 16:8 h light-dark photoperiod. To evaluate the effect of RSA on biological-based problems, the experiment was conducted in three phases. The benefits of utilizing RSA were established through the following observations: effective removal of contaminants at an acceptable level without releasing the culture; extension of the biofilm formation time on the inner walls; inhibition of heterotrophic bacteria and nitrification; enhancement of the suspended solids retention capacity of the raceway tank (up to 770 mg.L−1); and improvement in ammonium removal rate to approximately 30 mg.L−1d−1. The ideal salinity level for both ammonium removal and biomass concentration in RSA should be below 0.02%. These findings demonstrate the potential of phycoremediation for sustainable wastewater treatment and contribute to environmental bioremediation strategies.

Liquid-infused surface on optimized anodic porous aluminum for anti-frost and anti-ice application

Here, we present liquid-infused surfaces (LISs) fabricated on an aluminum substrate using the anodizing method to introduce specific nanofeatures infiltrated by silicone oil to reduce the friction between ice and surface. To achieve various nanostructures on the aluminum substrate, the anodizing conditions are varied. The ice adhesion stress could be significantly plummeted to 21.7 kPa on an LIS with optimized dimensions for nanofeatures. Frost formation tests in environments with a relative humidity of 75 % on the fabricated surfaces with a temperature of −17 ℃ reveal that the fabricated samples can considerably extend the frost formation time. The frosting time for the fabricated sample can reach 47 min, whereas the bare aluminum is completely covered by frost only after 1.5 min

A Deployable Viscoelastic Coagulation Monitor Enables Point-of-Care Assessment of Coagulopathy in Swine With Polytrauma.

Absence of pre-hospital coagulation tests challenges prompt management of hemostasis after trauma. The Viscoelastic Coagulation Monitor (VCM, Entegrion, Durham, NC) is a hand-held coagulation test for point-of-care. We evaluated VCM in a translational swine polytrauma model, hypothesizing that VCM correlates with a laboratory reference method, the TEG 5000 (Haemonetics, Boston, MA), and can identify coagulopathic phenotypes relevant to trauma. Our secondary hypothesis was that pre-warming of VCM disposable test cartridges using a heating plate versus pre-warming of cartridges by carrying the cartridge in the user's pocket does not significantly alter results. This study was conducted in tandem with a parent study involving anesthetized, mechanically ventilated swine (n = 20; 54 ± 5 kg) that encountered traumatic brain injury, pulmonary contusion and hemorrhage, or combination/polytrauma injury. Blood was collected at baseline, post-injury, post-shock, post-transfusion, and 6-, 24-, and 48 h post-injury to perform VCM at point-of-care. Within-group effect of time was assessed. Spearman correlation examined linear relations between VCM and standard laboratory-based coagulation tests; as well as lactate, ionized calcium, and body temperature. Logistic regression examined predictiveness of VCM to identify coagulopathic phenotypes, with receiver operator characteristic curves generated to assess diagnostic capability. At a subset of timepoints, necessity of pre-warming the VCM test cartridge using a heating plate versus pre-warming the cartridge by placement in the user's pocket was assessed by conducting simultaneous tests on two separate instruments, with results analyzed by paired t-test with crossover design. VCM revealed time-dependent changes in clotting time, clot formation time (CFT), alpha, maximum clot firmness (MCF), and lysis index (LI30). All VCM metrics correlated with the respective TEG 5000 metrics, with strongest correlation for VCM MCF with TEG MA (rhos = 0.77, P < .0001) and VCM LI30 with TEG LY30 (rhos = -0.76, P < .0001). VCM demonstrated good (area under the curve >0.70) to excellent (area under the curve >0.90) diagnostic accuracy in detection of low platelet count (MCF), low hematocrit (clotting time, clot formation time, alpha, and MCF), low fibrinogen (MCF), and high fibrinogen (alpha, MCF). There was no statistically or clinically relevant effect of cartridge warming method on results. In a trauma model, VCM detected significant changes in coagulation at point-of-care in a simplified portable form factor. VCM could enable informed hemostasis management in pre-hospital settings where coagulations tests are unavailable, pending further validation in clinical trials.

The HYENAS project: a prediction for the X-ray undetected galaxy groups

Abstract Galaxy groups contain the majority of bound mass with a significant portion of baryons due to the combination of halo mass and abundance (Cui 2024). Hence they serve as a crucial missing piece in the puzzle of galaxy formation and the evolution of large-scale structures in the Universe. In observations, mass-complete group catalogues are normally derived from galaxy redshift surveys detected through various three-dimensional group-finding algorithms. Confirming the reality of such groups, particularly in the X-rays, is critical for ensuring robust studies of galaxy evolution in these environments. Recent works have reported numerous optical groups that are X-ray undetected (see, e.g. Popesso et al. 2024), sparking debates regarding the reasons for the unexpectedly low hot gas fraction in galaxy groups. To address this issue, we utilise zoomed-in simulations of galaxy groups from the novel Hyenas project to explore the range of hot gas fractions within galaxy groups and investigate the intrinsic factors behind the observed variability in X-ray emission. We find that the halo formation time can play a critical role – we see that groups in halos that formed earlier exhibit up to an order of magnitude brighter X-ray luminosities compared to those formed later. This suggests that undetected X-ray groups are preferentially late-formed halos and highlights the connection between gas fraction and halo formation time in galaxy groups. Accounting for these biases in galaxy group identification is essential for advancing our understanding of galaxy formation and achieving precision in cosmological studies.

New probe of non-Gaussianities with primordial black hole induced gravitational waves

We propose a new probe of primordial non-Gaussianities (NGs) through the observation of gravitational waves (GWs) induced by ultra-light (MPBH<109g) primordial black holes (PBHs). Interestingly enough, the existence of primordial NG can leave imprints on the clustering properties of PBHs and the spectral shape of induced GW signals. Focusing on a scale-dependent local-type NG, we identify a distinct double-peaked GW energy spectrum that, contingent upon MPBH and the abundance of PBHs at the time of formation, denoted as ΩPBH,f, may fall into the frequency bands of upcoming GW observatories, including LISA, ET, SKA, and BBO. Thus, such a signal can serve as a novel portal for probing primordial NGs. Intriguingly, combining BBN bounds on the GW amplitude, we find for the first time the joint limit on the product of the effective non-linearity parameter for the primordial tri-spectrum, denoted by τ¯NL, and the primordial curvature perturbation power spectrum PR(k), which reads as τ¯NLPR(k)<4×10−20ΩPBH,f−17/9(MPBH104g)−17/9.

Clot waveform analysis of prolonged activated partial thromboplastin time in various disorders.

The prolongation in APTT reflects various disorders affecting the blood coagulation system. Automated coagulation analyzers such as ACL TOP, help in evaluating the clotting cascade by displaying clot reaction curves or waveforms. The fibrin formation curve forms S-shaped curve showing the changes in the absorbance on X-axis and time in seconds on Y-axis. The clotting data is processed to obtain derivatives. This observational study analyzed 80 samples including 20 samples from normal healthy individuals as control. The parameters studied were time (secs), height (mAbs), and width (secs) of first derivative (FD), fibrin clot time at ½ height, height (mAbs), and width (secs) of both first and second peaks of second derivative (SD). Hemophilia (n = 13) that consisted of factor VIII deficiency (n = 11) and factor IX deficiency (n = 2) showed biphasic patterns in both first DC and second DC. Cases with thrombosis (n = 8) showed marked elevation in first DC. Various other disorders (n = 39) that consisted of mainly hepatic dysfunction (n = 24) showed prolongation in fibrin formation, first DC, and second DC peak time. The atypical derivative curve analysis helps in determining the interferences in clotting cascade. It gives significant information and provides direction for further testing.

DAYA MULTIPLIKASI EKSPLAN KENTANG AR 8 PADA BERBAGAI KONSENTRASI BENZIL AMINO PURIN (BAP) DAN EKSTRAK BAWANG MERAH DALAM MEDIA DASAR MURASHIGE DAN SKOOG (MS) SECARA IN VITRO

The technique of propagating potato seeds through tissue culture has long been used to produce quality and disease-free seeds. This study aims to test the effect of the combination of BAP and Shallot Extract on the growth of potato plant explants on MS media (Murshige &amp; Skooge). Research in experimental form uses a completely randomized design (CRD) in a factorial pattern consisting of two factors. As the first factor, the growth hormone BAP consists of 4 levels, namely: 0 ppm, 2 ppm, 3 ppm and 4 ppm. Meanwhile, the second factor is: shallot extract consists of 4 treatment levels, namely: 0%, 0.2%, 0.4% and 0.6%. Thus, there were 16 treatment combinations which were repeated 6 times so that the total experimental units were 96. The ABA treatment with a concentration of 2 ppm/liter gave the best effect on the shoot formation time and root formation time of AR 8 potato plant explants. However, the treatment without ABA had a good effect on Shoot length and root length of explant potato plant varieties AR 8. Shallot extract treatment with a concentration of 20% had the best effect on shoot formation time, root formation time, shoot length and number of leaves. However, the treatment with a concentration of 40% had a better effect on the parameters of the number of shoots and the number of explant segments of the AR 8 variety of potato plants. The interaction of the ABA treatment with a concentration of 2 ppm and the shallot extract with a concentration of 20% had the best effect on the time of emergence of shoots and roots on the AR 8 Potato Explants. However, the interaction without ABA (0ppm) and shallot extract had a better effect on the parameters of shoot length, number of leaves, and number of explant segments in the AR 8 potato variety.

Verification of Tropical Cyclogenesis Forecasts of the Korean Integrated Model for 2020–21

Abstract Accurate tropical cyclogenesis (TCG) prediction is important because it allows national operational forecasting agencies to issue timely warnings and implement effective disaster prevention measures. In 2020, the Korea Meteorological Administration employed a self-developed operational model called the Korean Integrated Model (KIM). In this study, we verified KIM’s TCG forecast skill over the western North Pacific. Based on 9-day forecasts, TCG in the model was objectively detected and classified as well-predicted, early formation, late formation, miss, or false alarm by comparing their formation times and locations with those of 46 tropical cyclones (TCs) from June to November in 2020–21 documented by the Joint Typhoon Warning Center. The prediction of large-scale environmental conditions relevant to TCG was also evaluated. The results showed that the probability of KIM detection was comparable to or better than that of previously reported statistics of other numerical weather prediction models. The intrabasin comparison revealed that the probability of detection in the Philippine Sea was the highest, followed by the South China Sea and central Pacific. The best TCG prediction performance in the Philippine Sea was supported by unbiased forecasts in large-scale environments. The missed and false alarm cases in all three regions had the largest prediction biases in the large-scale lower-tropospheric relative vorticity. Excessive false alarms may be associated with prediction biases in the vertical gradient of equivalent potential temperature within the boundary layer. This study serves as a primary guide for national forecasters and is useful to model developers for further refinement of KIM.

How Does a Low Surface Brightness Galaxy Form Spiral Arms?

The formation and evolution of spiral arms in low surface brightness galaxies (LSBs) are not well understood. We study the dynamics of spiral arms in two prototypical LSBs, FGC 568-VI and FGC 568-01, using both analytical models and N-body + hydrodynamical simulations. We first consider the disk as a two-component system of gravitationally coupled stars and gas in the force field of a spherical dark matter halo, subjected to local, nonaxisymmetric perturbations. However, no local spirals are formed. We next assume the disk to be a one-component system of stars in the net gravitational potential of a galaxy with a spherical dark matter halo perturbed by a global m = 2 instability. In this case, the growth time for spiral formation was low, equal to 0.78 and 0.96 Gyr, respectively, corresponding to a few dynamical times of the galaxies. Finally, we simulate the LSBs using the N-body + hydrodynamical simulation code RAMSES. Our results show that a quadrupolar field associated with an oblate halo with an axial ratio of 0.7 is necessary to drive a long-lived global spiral in the LSB disks. Further, feedback corresponding to a supernova mass fraction of ∼0.05 is essential to comply with the observed stellar surface density. The simulated spirals survive for about 10 dynamical times and the average pattern speed lies between 10 and 15 km s−1 kpc−1. The spiral arms thus formed are therefore a transient global pattern driven by the tidal field of the oblate dark matter halo.

Development of in situ forming autologous fibrin scaffold incorporating synthetic teriparatide peptide for bone tissue engineering.

This study investigates the potential of an in-situ forming scaffold using a fibrin-based scaffold derived from autologous plasma combined with Synthetic Teriparatide (TP) for bone regeneration application. TP is known for its bone formation stimulation but has limited clinical use due to side effects. This autologous delivery system aims to provide precise, controlled, localized, and long-term release of TP for accelerating bone regeneration. Fibrinogen from autologous plasma was extracted using ethanol, and thrombin was precipitated with ammonium sulfate to create the fibrin scaffold. Characterization of fibrinogen was done through FTIR, SDS-Page, porosity, SEM, degradation, and rheology tests. Viability was assessed by MTT in five groups with different concentrations of TP in fibrin scaffold (50, 100, and 150 µl/ml), fibrin alone, and a control group against HEK and Wharton's jelly cells. The release profile of different concentrations of TP in the fibrin scaffold was also examined. The formation time of the fibrin scaffold was 4 ± 0.2 s. The highest Infrared absorption for fibrinogen was confirmed. Rheology assessment revealed a higher elastic modulus than the viscous modulus. The created fibrin scaffold displayed a consistent three-dimensional microstructure with an interconnected porous network. Cytotoxicity assays demonstrated good biocompatibility and enhanced cell growth with different concentrations of TP in the fibrin scaffold. The TP release increased with higher concentrations, peaking at an average of 61% over 54 h. Autologous plasma-derived fibrin scaffolds incorporating TP exhibit satisfactory release within the scaffold and hold promise as a versatile bone filler for clinical use, facilitating osteoregeneration.

Prospective cohort study of rotational thromboelastometry in established biliary obstruction: dispelling the myth of auto-anticoagulation

BackgroundPatients with obstructive jaundice are conventionally described as hypocoagulable due to vitamin K malabsorption. However, associated underlying malignancy and synthetic liver dysfunction are mediators of hypercoagulability. The actual effect of biliary obstruction on the coagulation profile is not well characterised. This study aimed to define the coagulation status of patients with established biliary obstruction using rotational thromboelastometry (ROTEM). MethodsThis prospective cohort study, conducted in an Australian metropolitan hospital, included patients with a total bilirubin level of >150 umol/L and biliary obstruction on imaging. The primary outcome was the coagulation profile assessed using ROTEM. Results20 patients were included (median age 74.5 years), 15 were male and 17 had a malignant cause for biliary obstruction. The median bilirubin level was 209 umol/L (IQR: 175.0 umol/L – 255.8 umol/L). On ROTEM, all patients had normal or reduced clot formation times, and normal or increased maximum clot firmness. This confirmed all patients had a normal or hypercoagulable clotting profile, and none were auto-anticoagulated. Vitamin K administration before ROTEM did not vary the coagulation profile. DiscussionPatients with established biliary obstruction and jaundice, predominantly due to malignancy, were normo or hypercoagulable. The belief that obstructive jaundice is associated with a hypocoagulable state should be questioned.

Improving the gel properties of Ficus pumila Linn. pectin by incorporating deacetylated konjac glucomannan

To improve the gelation behaviour of pectin, the effect of deacetylated konjac glucomannan (DKGM) with various deacetylation degrees (27.44 %, 44.32 %, 60.25 %, and 71.77 %) on the heat-induced gel characteristics of Ficus pumila Linn. pectin was studied. The hardness, chewiness, and adhesiveness of the gel increased as the degree of deacetylation increased from 27.44 % to 60.25 %, but decreased at 71.77 %. Additionally, DKGM addition resulted in higher apparent viscosity and non-Newtonian fluid behaviour in the composite gel. The incorporation of DKGM into the gel matrix strengthened the gel structure by promoting hydrogen bond formation and shortening relaxation time compared to the control. Scanning electron microscopy images revealed that the densification of the pectin gel network increased as the degree of deacetylation of konjac glucomannan rose from 27.44 % to 60.25 %, but then loosened when it exceeded 71.77 %. As the degree of deacetylation increased, the hydrophobic interaction between pectin and DKGM increased. Overall, the addition of DKGM effectively modulated the gel properties of Ficus pumila Linn. pectin, thus broadening its industrial application on different gel products.

Enhanced antifouling behavior and phosphorus removal in a gravity-driven electrochemical dynamic membrane bioreactor (EDMBR) process: Performance and mechanisms

Gravity-driven dynamic membrane bioreactor (DMBR) process has emerged as a promising wastewater treatment technology. However, long dynamic membrane (DM) formation time, membrane fouling and low phosphorus removal have limited its widespread application. A novel gravity-driven electro-DMBR (EDMBR) using stainless-steel meshes as the anodic membrane and graphite plates as the cathodes was developed for domestic wastewater treatment, with process performance, DM properties and energy consumption compared with a control DMBR without external voltage applied. Electrostatic adsorption between negatively charged foulants and positively charged anodic membrane shortened DM formation time to less than 4 min. Afterwards, electro-oxidation effect and biodegradation effect resulted from the enrichment of electroactive microorganisms (such as Pseudomonas, Acinetobacter, Exiguobacterium) ensured continuous degradation of attached foulants and maintained a thin and well-structured DM layer, showing higher stable flux and reduced cleaning frequency at the voltage of 1.2 V and 1.6 V compared to the control DMBR. The electro-coagulation (EC) effect originated from iron releasing modified the sludge properties and enhanced phosphorus removal (74 %-87 %). Moreover, the EDMBR with higher permeability and treatment capacity resulted in lower energy consumption (0.41–0.44 kWh/m3) compared to the DMBR (0.50 kWh/m3). The results provide new insights into sustainable operation of the EDMBR process towards lower energy demand and less maintenance requirement.

Low-temperature plasma jet treatment generates reactive oxygen species in solution that leads to peptide oxidation and protein aggregation

Abstract Low-temperature plasma (LTPs) jets are FDA-approved medical devices to remove cancerous tissue and aid in wound healing. However, reports on their reaction with proteins are conflicting, ranging from fragmentation, oxidation, aggregation, or a combination thereof. In this study we bridge the gap between plasma-treatment of short peptides to proteins at physiologically relevant concentrations. The LTP in this study is based on a helium dielectric barrier discharge (DBD) that forms a plasma-jet, which is directed at the solution without direct contact with the plasma, and results in the formation of reactive oxygen species (ROS) OH• and O2•- in solution. The longer the solution is treated, the more solution-phase ROS form. Treating peptide- and protein-containing solutions leads to extensive oxidation. The ROS led to the same oxidative modifications for peptide M with increasing chain length (9, 18, 37, 76 amino acids), which could be identified with high-resolution mass spectrometry. Oxidized species M+xO led to conformational changes such as compaction and elongation, while the unmodified peptide M remained unaltered, as found by ion mobility spectrometry and size exclusion chromatography. For proteins at high concentration, insoluble aggregates formed and could be identified by UV/Vis light scattering and atomic force microscopy. The formation of aggregates is dependent on the amino acid chain length, the peptide concentration, and the time for aggregate formation. These findings highlight the importance of both peptide chain length and concentration in determining the fate of peptides following the exposure to LTP, while also offering valuable insights for the field of plasma medicine. &amp;#xD;

Tocilizumab reduces hypercoagulation in COVID-19 – Perspectives from the coagulation and immunomodulation Covid assessment (Coag-ImmCovA) clinical trial

BackgroundDespite medical interventions, COVID-19 continues to persist at pandemic proportions. A hypercoagulation state was rapidly observed in the severely ill, and the incidence of thromboembolic events remains elevated. Interleukin inhibitors have demonstrated positive effects on the hyperactivation of the immune system in COVID-19, with the interleukin-6 inhibitor tocilizumab showing promising results in reducing mortality. Nevertheless, the impact of interleukin inhibitors on the coagulation system remains incompletely understood. MethodsIn this clinical trial conducted in Stockholm, Sweden, interleukin inhibitors, namely anakinra (ANA) or tocilizumab (TOCI), were randomly administered in addition to standard care (SC) to hospitalized patients with COVID-19. A control group received only SC. The primary outcome sought to measure effects on global hemostasis, as indicated by changes in functional coagulation tests, specifically Rotational Thromboelastometry (ROTEM) or Overall Hemostatic Potential (OHP), visualized through scanning electron microscopy images. Secondary outcomes included effects on conventional coagulation laboratory tests. ResultsThe study enrolled 74 patients who were randomized to receive either ANA or TOCI in addition to SC, or SC alone. In the TOCI group, ROTEM variables exhibited less hypercoagulation after 29 days compared with ANA or SC treatment groups, characterized by prolonged clot formation time and decreased clot firmness. OHP decreased, but there were no significant differences among the three treatment groups. Plasma fibrinogen levels, initially elevated, decreased significantly in TOCI recipients over time. ConclusionTocilizumab treatment demonstrated a significant reduction of hypercoagulation in hospitalized COVID-19 patients, by improvements in both global coagulation tests and conventional laboratory tests, in comparison with anakinra or SC alone. This finding underscores the significance of tocilizumab as a viable treatment option in severe COVID-19 cases, with the potential to decrease thrombosis incidence.