Tilapia Fish Research Articles

Scale Morphology and Population Differentiation in Exotic Fish Tilapia (Oreochromis mossambicus P. 1852) from Some Major Water Bodies of Western India

Scale Morphology and Population Differentiation in Exotic Fish Tilapia (Oreochromis mossambicus P. 1852) from Some Major Water Bodies of Western India

Nanoparticles fabricated from the bioactive tilapia scale collagen for wound healing: Experimental approach.

The creation of innovative wound-healing nanomaterials based on natural compounds emerges as a top research goal. This research aimed to create a gel containing collagen nanoparticles and evaluate its therapeutic potential for skin lesions. Collagen nanoparticles were produced from fish scales using desolvation techniques. Using SDS PAGE electrophoresis, Fourier transform infrared spectroscopy (FTIR) as well as the structure of the isolated collagen and its similarities to collagen type 1 were identified. The surface morphology of the isolated collagen and its reformulation into nanoparticles were examined using transmission and scanning electron microscopy. A Zeta sizer was used to examine the size, zeta potential, and distribution of the synthesized collagen nanoparticles. The cytotoxicity of the nanomaterials was investigated and an experimental model was used to evaluate the wound healing capability. The overall collagen output from Tilapia fish scales was 42%. Electrophoretic patterns revealed that the isolated collagen included a unique protein with chain bands of 126-132 kDa and an elevated beta band of 255 kDa. When compared to the isolated collagen, the collagen nanoparticles' FTIR results revealed a significant drop in the amide II (42% decrease) and amide III (32% decrease) band intensities. According to SEM analysis, the generated collagen nanoparticles ranged in size from 100 to 350 nm, with an average diameter of 182 nm determined by the zeta sizer. The produced collagen nanoparticles were polydispersed in nature and had an equivalent average zeta potential of -17.7 mV. Cytotoxicity study showed that, when treating fibroblast cells with collagen nanoparticle concentrations, very mild morphological alterations were detected after human skin fibroblasts were treated with collagen nanoparticles 32 μg/ml for 24 hours, as higher concentrations of collagen nanoparticles caused cell detachment. Macroscopical and histological investigations proved that the fabricated fish scale collagen nanoparticles promoted the healing process in comparison to the saline group.

Study on the plant and fish production in the aquaponic system as affected by different hydraulic loading rates

Aquaponics is the combined culture of fish and plants in recirculating aquaculture systems, considered to be an innovative, eco-friendly and sustainable technology. The effect of the hydraulic loading rate (HLR) on the performance of fish and plants in the aquapoinc system was the main aim of this study. Four hydraulic loading rates were applied, 1.2, 1.8, 2.4 and 3.0 m day−1 under stocking density tilapia fish of 5 kg m−3 and lettuce population of 25 plant m−2 for a period of January to March, 2023. Water parameters, plant and fish parameters were determined. The most important results revealed that the highest plant nutrients removal was at HLR of 2.4 m day−1. The highest value of water parameters were found at the HLR of 2.4 m day−1. Root length increased with increasing HLR. Fresh and dry shoot and root weight values were higher at 2.4 m day−1 compared to other treatments under study. Meanwhile, fish growth parameter showed higher values at the HLR of 3.0 m day−1 compared to other treatments. The highest values of weight gain, feed growth rate, specific growth rate and feed conversion ratio were 81.72 g, 1.36 g day−1, 1.88% day−1 and 1.20 g feed g−1 fish, respectively, for all treatments under study.

Fabrication Of Butterfly Pea Flower Anthocyanin-Incorporated Colorimetric Indicator Film Based On Gelatin/Pectin For Monitoring Fish Freshness

Novel visual intelligent pH-indicator film was prepared from the eggshell membrane- gelatin, pectin, and anthocyanin pigment from butterfly pea flower (BP) (Clitoria ternatea). It was used as a real-time pH indicator for predicting food freshness. Eggshells are useful biowaste, gelatin has been extracted from the eggshell membrane and was used for fabricating the film. The Anthocyanin content of BP extract was 198.3 mg g−1. The film's surface morphology and chemical nature were estimated using a Scanning electron microscope, Fourier transform infrared spectroscopy, and X-ray diffraction technique. The film was pH responsive and exhibited color variation ranging from shades of red, purple, blue, green, and yellow at different pH (1-13). The applicability of the developed pH- indicator film was studied on fresh Tilapia fish by monitoring its deterioration for a specific time period. The film showed a visible color change after seven days of storage at 4ºC from dark blue, bluish-grey to olive, and deep green. Change in the total volatile basic nitrogen (TVB-N) content and pH change had an effect on the color response of the film. The findings demonstrated that the fabricated pH indicator film proved to be pH sensitive and could be used to monitor fish freshness.

Tilapia Fish Skin Treatment of Third-Degree Skin Burns in Murine Model.

This study explored the feasibility of using fish skin bandages as a therapeutic option for third-degree skin burns. Following the California wildfires, clinical observations of animals with third-degree skin burns demonstrated increased comfort levels and reduced pain when treated with tilapia fish skin. Despite the promises of this therapy, there are few studies explaining the healing mechanisms behind the application of tilapia fish skin. In this study, mice with third-degree burns were treated with either a hydrocolloid adhesive bandage (control) (n = 16) or fish skin (n = 16) 7 days post-burn. Mice were subjected to histologic, hematologic, molecular, and gross evaluation at days 7, 16, and 28 post-burn. The fish skin offered no benefit to overall wound closure compared to hydrocolloids. Additionally, we detected no difference between fish skin and control treatments in regard to hypermetabolism or hematologic values. However, the fish skin groups exhibited 2 times more vascularization and 2 times higher expression of antimicrobial defensin peptide in comparison to controls. Proteomic analysis of the fish skin revealed the presence of antimicrobial peptides. Collectively, these data suggest that fish skin can serve as an innovative and cost-effective therapeutic alternative for burn victims to facilitate vascularization and reduce bacterial infection.

Relationship Between Length and Weight and Habitat Conditions of Several Fish Species in Tondano Lake, Minahasa, North Sulawesi

The snake-head gudgeon or payangka fish (Ophieleotris aporos), tilapia fish or mujair (Oreochromis mossambicus), and marble goby or betutu fish (Oxyeleotris marmorata) are economically important fish in Lake Tondano. However, numerous community activities can disrupt aquatic biodiversity, declining Lake Tondano environmental conditions, and other factors. This study aimed to determine the relationship between length and weight and the habitat conditions of Ophieleotris aporos, Oreochromis mossambicus, and Oxyeleotris marmorata. The method used in this study was purposive sampling with research stations located at Remboken (station 1), Kakas (station 2), and Tondano Estuary (station 3). Sampling was carried out 5 times in every 10 days. The weight and length of the fishes caught in Lake Tondano were 2-60 g and 1-8 cm (Ophieleotris aporos); 9-180 g and 6-25.5 cm (Oreochromis mossambicus); and 16-30 g and 2.6-26.5 cm (Oxyeletotris marmorata), all of them have an allometric growth pattern. Oreochromis mossambicus is the most commonly caught fish in all research stations. The research station with the best level of water fertility is Remboken Station. The habitat characteristic of Lake Tondano was 25-290C of water temperature; brightness of 1.34-2.66 m; depth of 4.23-11.8 m; pH of 6.7-7.8; and dissolved oxygen of 2.27-6.31 mg/L, which still supports the well-being of the fish.

Levels of heavy metals in water and Nile tilapia fish (<i>Oreochromis niloticus</i>) of Eleyele Lake in Ibadan, Nigeria

Surface waters are susceptible to hazardous pollution which may migrate with devastating health challenges through the food chain to humans. This study evaluates the levels of heavy metals in water and Nile tilapia of Lake Eleyele. The physicochemical properties of water samples were determined using standardized methods, while heavy metals in both water and fish samples were determined using atomic absorption spectrophotometer (AAS). Water pH, electrical conductivity, total dissolved solids, total suspended solids, and dissolved oxygen were within WHO and USEPA limits for potable water. Cu (0.028 – 0.112 mgL-1), Pb (0.002 – 0.009 mgL-1), and Zn (1.46 – 3.79 mgL-1) were below the maximum permissible limits by both WHO and USEPA, while cadmium, chromium, and nickel were not detected in the water samples. Cu (2.56 -3.54 mg/kg), Zn (22.93 – 32.13 mg/kg), and Ni (0.39 – 0.48 mg/kg), are within acceptable levels, while Cd and Cr were also not detected in the fish samples. However, Pb (1.71 – 2.30 mg/kg) was higher in the fish samples than the threshold limit (0.3 mg/kg) by FAO and WHO. In accordance, Pb with bioconcentration factor (BCF: 402) was more highly bio-accumulated in the fishes than Cu (BCF: 54.4) and Ni (BCF: 10.97). The results recommend the river water for utility purposes but raised concerns about the consumption of Nile tilapia fishes in the lake.

The Effect of Adding Gotu Kola Leaves (Centella asiatica L.) on the Nutrient Content of Tilapia Fish Meatballs (Oreochromis niloticus) as a Healthy Snack for Teenagers

One of the eating behaviors in teenagers is the habit of snacking in the form of snacks. Snacks have a negative impact if consumed excessively, namely they can cause excess energy intake. The aim of this research was to determine the sensory evaluation and nutritional content of tilapia fish meatballs with the addition of gotu kola leaf flour. This research used a Completely Randomized Design (CRD) with four treatments and three repetitions. Four treatments were used in making fish meatballs with the addition of gotu kola leaf flour, namely 10 gr; 15 gr; 20 gr; 25 gr. The results of the research showed that the best fish meatballs were the treatment concentration of 5% gotu kola leaf flour which had an energy content of 223.37 kcal, protein 45.2 grams, fat 0.29 grams, carbohydrates 10.08 grams, water content 62.8%, ash content 1.33% and fiber 2.36%.

Molecular Characterization Of Antimicrobial Resistance In Edwardsiella Tarda Isolated From Diseased Tilapia Fish In Egypt

Molecular Characterization Of Antimicrobial Resistance In Edwardsiella Tarda Isolated From Diseased Tilapia Fish In Egypt

The toxicity effects of the individual and combined exposure of methyl tert-butyl ether (MTBE) and tire rubber powder (RP) on Nile tilapia fish (Oreochromis niloticus)

Methyl tert-butyl ether (MTBE) is soluble in water and can contaminate water sources when it spills during transportation or leaks from underground storage tanks. Incomplete combustion releases MTBE as exhaust fumes that can be deposited on urban surfaces. Meanwhile, car tires erosion emits of large amounts of rubber dust (RP), easily transported to water bodies. Therefore, this study has the objective of assessing the toxicity of varying concentrations of MTBE (0, 2.5, 5.0 μL L−1) and RP (0, 5.0, 10.0 mg L−1 RP), both individually and in combination, over a period of 28 days on Nile tilapia (Oreochromis niloticus). MTBE and PR decreased fish growth performance. Blood biochemical analytes indicated that MTBE and RP led to increasing Aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), and creatinine phosphokinase (CPK), alkaline phosphatase and gamma-glutamyl transferase (GGT) activities. Alterations related to glucose, triglycerides, cholesterol, and creatinine, plasma contents, were also observed. Increased antioxidant biomarkers, including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), glutathione reductase (GR), and malondialdehyde (MDA), was observed. Exposure fish to MTBE and PR changed metabolic profile of muscle tissue. Moreover, results showed that MTBE, its metabolites, and PR could accumulate in the muscle tissue of fish. Results suggest that MTBE and RP can impact fish health, both individually and when combined. The presence of MTBE enhances the toxicity of RP, indicating a synergistic effect. Nevertheless, further studies are needed to understand the impact of toxic compounds on aquatic environments and organisms' health.

COMMUNITY'S PERCEPTION OF POST-TIMAH MINING LAND MANAGEMENT AS AIK NIBUNG ARD GARDEN AGRO-ECOTOURISM IN BADAU VILLAGE

The development of Aik Nibung agro-ecotourism ARD Garden in Badau Village is a post-tin mining management business. This activity cannot be separated from the mainstay role of farmers in this management. This study aimed to identify various forms of business management of post-tin mining land by leading farmers and to describe community perceptions of post-tin mining land management in Badau Village. This research is a case study with a descriptive research method and is analyzed qualitatively using a Likert scale. Sampling was done purposively (purposive sampling) with a total of 30 respondents. Data was collected through questionnaires, observations, interviews, and documentation. The results showed that: 1) The business managed by farmers is the mainstay in developing the Aik Nibung agro-ecotourism area ARD Garden, namely celocia, sunflower and button flower gardens, horticultural plant cultivation and tilapia fish cultivation; 2) The community's perception of pre-management of post-tin mining land is in agreement with an index coefficient value of 78.2%. Community perceptions of post-mining land management agree with an index coefficient value of 78.7% and community perceptions of successful post-mining land management strongly agree with an index coefficient value of 83.17%. With an average index coefficient value of 80.02% at the level of strongly agree.

Evaluation of Heavy Metals in Catfish and Tilipia Fish from Bayelsa River Nigeria

The volume of heavy metals in two different fish samples were examined in this report, dry ashing was adopted to digest the samples and the determination of the heavy metals were evaluated using atomic absorption spectrophotometer. from the analyzed sample the concentration of cadimium in all the samples analyzed were below WHO permissible limits which is 0.2 mg/kg, From the different analyzed samples the concentration of lead was below the WHO’s allowable limits which is 0.05 mg/kg, however, catfish and tilapia fish samples gotten from Amasomma have the highest concentration of lead 0.0042 ±0.0021 and 0.0030 ±0.001 respectively. The concentration of cadmium, Aluminium and Manganese are below WHO permissible limits for these heavy metals.

Exploring the Impact of Probiotics on the Gut Ecosystem and Morpho-Histology in Fish: Current Knowledge of Tilapia

As global demand for fish as a food source continues to rise, addressing challenges within the aquaculture industry and meeting consumer needs becomes imperative. Enhancing fish growth and safeguarding against microbial pathogens are among these challenges. Understanding the intestinal microbiome holds promise for achieving these goals and benefiting the aquaculture sector with a particular focus on tilapia, which has gained increased attention in recent research. Probiotics, live microorganisms administered at appropriate levels, can foster a balanced gut microbiota in animals. This can yield advantages like pathogen defence, improved digestion, enhanced growth, and increased survival rates in Tilapia. Probiotics are emerging as tools to promote healthy intestinal environments in Tilapia and other fish species, with potential to positively influence gut structure, ecosystem, growth, and disease resistance. The dynamic interplay between tilapia and their intestinal microbiota has garnered significant attention, affecting tilapia's health, performance, and overall well-being. This review compiles research findings on the influence of probiotics on tilapia intestinal ecosystems, morpho-histological structures, and enzymatic activities. It outlines their impact on gut microbiota composition in tilapia, demonstrating their ability to increase beneficial bacteria while reducing pathogenic strains. Probiotics can enhance intestinal morphology in tilapia, promoting villi development, goblet cell density, and mucous layer thickness. Additionally, they influence digestive enzyme activity in tilapia, resulting in improved nutrient absorption and tilapia growth. Although this field is continually evolving, the progress in deciphering probiotics' complex interactions with tilapia guts underscores their potential benefits in advancing tilapia aquaculture practices.

Effects of a free amino acid mixture in replacing dietary fishmeal and reducing Nile tilapia (Oreochromis niloticus) production costs

To encourage aquaculture sustainability and lower fish production costs, fish products need to be substituted with sustainable ingredients in aquafeeds. Toward this goal, we examined whether a natural mix of highly concentrated free amino acids could alleviate the negative effects of low–fish meal (FM) diets on Nile tilapia in terms of growth performance and nutrient utilization. In the present study, a 2 × 3 factorial design with two FM levels (5 % and 10 %) was used to prepare the experimental diets and three levels of Kera-Stim® 50 (KS), a free amino acid mixture, was supplemented (0 %, 0.25 %, and 0.5 %). Nile tilapia fish (average initial body weight of 20 g) were cultured in 18 cages and provided with one of the six diets for eight weeks. We found that dietary KS supplementation significantly increased the growth performance parameters including final body weight, weight gain, and specific growth rate compared with the diets without KS supplementation (P < 0.05). Moreover, KS supplementation had a substantial positive effect on the feed conversion ratio (P < 0.05). Interestingly, not only the low-FM diets but also the diets supplemented with KS had a positive effect on the economic conversion ratio and economic profit index, suggesting increased economic efficiency. Furthermore, lysozyme, catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase activity levels were significantly increased by supplementing low-FM diets with KS (P < 0.05). KS and low-FM diets increased the whole-body levels of ash, fat, and crude protein (P < 0.05). Moreover, KS supplementation increased the levels of polyunsaturated fatty acids, such as arachidonic acid (20:4 n6) and docosahexaenoic acid (C22:6 n3). We concluded that supplementing a natural free amino acid mix to a low-FM diet can mitigate negative nutritional effects and achieve optimum fish performance without compromising growth performance or revenue-to-cost ratios.

Understanding tilapia mortalities and fish health management in Lake Volta: a systematic approach

Unusual fish mortalities in aquaculture threaten global food security and carry significant socio-economic burdens. In 2018, Nile tilapia (Oreochromis niloticus) suffered unusual patterns of mortalities, attributed to disease-causing agents in Lake Volta cage aquaculture. In recent times, disease investigations have shifted from single to consideration of multiple factors to understand the puzzling range of causal risk factors. This study therefore aimed at expanding on tilapia mortality risk factors, while documenting fish health and Lake Volta management practices for sustainable aquaculture. We interviewed relevant aquaculture stakeholders operating on Lake Volta and conducted thematic analysis on their responses to map out mortality risk factors and management practices. The identified risk factors were conceptualized in established models of causation web and Social-Ecological System to explain the practical significance of the findings. The results showed that the risk factors of tilapia mortalities are a combination of pathogens and non-infectious factors mediated by weak law enforcement. The results further suggested mortality reinforcing mechanisms through the horizontal transmission of pathogens, namely, Streptococcus agalactiae and Infectious Spleen and Kidney Necrosis Virus. Moreover, the recognition of weak enforcement as a possible factor reinforcing human activities is a non-infectious route that can be deleterious to fish health. Health management practices comprised phytotherapy, vaccination, heat shock treatment, biological controls, and best husbandry practices. Lake management involves creating a waterfront buffer of 85.34 m, surveillance, and executing the framework guiding aquaculture development on the Lake. The findings are suggestive of complementary quantitative studies that augment the qualitative evidence herein. Such follow up studies can disclose precise mortality risk factors to inform policy directives and effective remedial strategies that can secure fish and lake health.

Physiological and immune profiling of tilapia Oreochromis niloticus gills by high-throughput single-cell transcriptome sequencing

The physiological and immune functions of fish gills are largely recognized, but their following functional heterogeneity at the single cell scale has been rarely reported. Here, we performed single cell RNA sequencing (scRNA-seq) on the gills of tilapia fish Oreochromis niloticus. We identified a total of 12 cell populations and analyzed their functional heterogeneity. To investigate the physiological function of O. niloticus gills, expression patterns of genes encoding ion transporters were selected from the identified H+-ATPase-rich cells (HR cells), Na+/K+-ATPase-rich cells (NaR cells), and pavement cells. Specific enrichment of ca4a, slc9a1a, and LOC100692482 in the HR cells of O. niloticus gills explained their functions in acid-base regulation. Genes encoding Ca2+ transporters, including atp2b1, LOC100696627, and LOC 100706765, were specifically expressed in the NaR cells. Pavement cells were presumably the main sites responsible for ammonia and urea transports in O. niloticus gills with specific enrichment of Rhbg and LOC100693008, respectively. The expression patterns of the four immune cell subtypes varied greatly, with B cells being enriched with the most immune-related GO terms. KEGG enrichment analysis showed that MAPK signaling pathway was the most enriched pathway among the four types of immune cells in O. niloticus gills. Our results are important in understanding the physiological and immune responses of fish gills at the cellular resolution.

Ultrasensitive detection of methicillin-resistant Staphylococcus aureus using a T7 exonuclease-assisted PAM-free dual CRISPR-Cas12a biosensor

Methicillin-resistant Staphylococcus aureus (MRSA) strains are prevalent foodborne superbugs and pose significant pathogens for both humans and animals. The sensitive and specific diagnosis of MRSA holds paramount importance for its prevention and treatment. Herein, we develop a T7 exonuclease-assisted PAM-free Dual CRISPR-Cas12a Biosensor (TDCB) targeting the antibiotic resistance gene MecA for ultrasensitive and highly specific MRSA detection. After generation of single-stranded DNA (ssDNA) obtained with T7 exonuclease digestion of amplified MRSA genome, the trans-cleavage activity of dual-Cas12a-crRNA complexes was initiated without the requirement of protospacer adjacent motifs (PAM). The fluorescent signal obtained with TDCB biosensor was stronger, compared with the single-Cas12a biosensor. TDCB detects the MRSA genome and cells with a high sensitivity of 100 ag/mL and 1 cfu/mL, respectively. Moreover, TDCB can distinguish MRSA from tested 6 non-MRSA bacterial species from each other. We further validated TDCB using MRSA-infected tilapia fishes and obtained results consistent with the qPCR assay. This TDCB technology, a novel Cas12a-based biosensor that detects genomic regions without reliance on PAM motifs, can have very broad utilization in hospitals, food and feed industry, and fish and dairy farming.

Kajian Bioekologi Ikan Dominan Sebagai Bioindikator Kesehatan Lingkungan Di Muara Sungai Kumbe Kabupaten Merauke

River estuaries are a habitat for various aquatic-associated organisms both in the water, on the soil surface and that immerse themselves in the soil. The purpose of this study was to study the bioecology of fish as a bioindicator of environmental health in the Kumbe River Muara, Merauke Regency. This research took place from September to November 2022, carried out along the Kumbe River estuary, Merauke Regency. The data collection method was carried out by purposive sampling and interviews. The results showed that the most abundant species found was snapper (Lates Calcarifer) while the least found was parang fish (Chirocentrus dorab). The diversity index shows the medium category, the uniformity value shows the community is stable and the dominance index shows the dominance value in the area is high. The environmental parameters measured were pH, temperature and salinity. The results showed that it was still in accordance with the quality standards for aquatic biota. The long-weight relationship based on representative fish was found to be dominant, namely snapper (Lates Calcarifer), tilapia (Oreochromis niloticus) and spiny fish (Arius sp.) showing a negative allometric growth pattern. Allometric growth shows that the length growth is not balanced with the weight growthKeywords : Bioecology, Fish, Kumbe River

Effect of chemical modification of κ-carrageenan on its inhibitory effect against heterocyclic amine (HAs) formation in roasted tilapia fish patties

Sulfated κ-carrageenan (S-KC), carboxymethylated κ-carrageenan (C-KC), acetylated κ-carrageenan (A-KC) and phosphorylated κ-carrageenan (P-KC) were synthesized and tested for their inhibitory effect on heterocyclic amine (HAs) formation in roasted tilapia fish patties. Fish patties with 1 % of each hydrocolloid prepared by 90 % of fish and 10 % of an aqueous hydrocolloid dispersion were determined for HAs-levels after roasting. P-KC showed the strongest inhibitory effect against total HAs formation (20.95 %). Moreover, P-KC increased the content of creatinine and glucose but decreased the content of free amino acids in fish patties, indicating that P-KC may compete with creatinine and glucose to react with amino acids to suppress HAs generation. In addition, P-KC plus naringenin had a stronger inhibitory effect against HAs formation than P-KC or naringenin alone. P-KC at 1 % (w/w) and P-KC (0.5 %, w/w) plus naringenin (0.5 %, w/w) showed no significant effects on the color and textural properties compared to the control group (100 % fish), and had less impact on food quality than 1 % (w/w) KC. Therefore, our results suggest that chemical modification could enhance the inhibitory effect of some hydrocolloids on HAs formation, and an appropriate combination of hydrocolloids and flavonoids contributes to the attenuation of dietary exposure to genotoxic HAs.

Use of Nile tilapia fish skin in treatment for burn victims

Burn injuries in the United States result in 1.1 million hospital admissions, with around 4500 deaths annually, according to the CDC. Although skin grafts have been the standard treatment since 1869, recent research suggests that tilapia skin could offer a safer and more effective alternative. This review aims to assess the strengths and knowledge gaps related to the usage of fish skin in burn patients. A systematic search was conducted on electronic databases, such as PubMed, Scopus, Web of Science, Clinicaltrials.gov, and Google Scholar, using relevant MESH keywords from 2018 to 2022. The search and analysis followed the “Preferred Reporting Items for Systematic Reviews and Meta-analysis” methodology. Data extraction utilized a template based on the Cochrane Consumers and Communication Review group’s extraction guidelines. Out of 166 studies identified, 12 relevant studies were extensively analyzed. The trials consistently demonstrated accelerated reepithelialization, improved patient compliance in managing burn-related pain, no immunologic or allergic reactions, reduced infection rates, shorter hospital stays, and decreased treatment costs when using fish skin. These findings suggest that fish skin is comparable to traditional treatments in terms of effectiveness and safety. Considering the discussed advantages, Nile tilapia fish skin emerges as a promising xenograft option for burn victims. However, further large-scale trials and studies are needed before its widespread and official implementation in burn centers. More research is required to establish the efficacy and safety of fish skin as a viable treatment modality.