Thrombogenic Indices Research Articles

Invasive Crayfish Faxonius limosus: Meat Safety, Nutritional Quality and Sensory Profile.

The aim of the present study was to evaluate the safety parameters, nutritional value and sensory profile of the meat from spiny-cheek crayfish (Faxonius limosus), captured from the Danube River in Serbia. To achieve this, we determined their microbiological safety, chemical composition, minerals and heavy metals, fatty acid and amino acid profile, as well as a sensory profile of the meat. The obtained results showed that the meat from crayfish was microbiologically safe. Crayfish meat has a high nutritional quality, high protein content (18.12%) and a total of 17 detected amino acids, of which essential amino acids constituted 6.96 g/100 g sample. Additionally, the crayfish meat was characterized by high levels of essential polyunsaturated fatty acids (PUFA), particularly n-3 PUFA, at an optimal ratio of n-3/n-6 and with low values of atherogenic and thrombogenic indices. Predominant macrominerals in the meat are K, followed by Na, Ca, P and Mg, whereas the content of microminerals was in the following order: Zn > Cu > Fe > Mn. The concentrations of accumulated toxic metals (Cd, Pb, As and Hg) did not exceed the maximum allowed levels. Sensory analysis confirmed that the meat from spiny-cheek crayfish has the potential to become a new food source of essential nutrients.

Lesser mealworm (Alphitobius diaperinus L.) larvae oils extracted by pure and binary mixed organic solvents: Physicochemical and antioxidant properties, fatty acid composition, and lipid quality indices

Insect oil is one of the most sustainable lipid sources with remarkable health effects. Herein, the type of organic solvents (i.e., n-hexane, ethanol, and isopropanol) and their binary mixtures was evaluated based on the quantity (e.g., yield extraction) and quality (e.g., bioactive compounds, thermal stability, DPPH scavenging rate, fatty acid profile, and nutritional indices) of lesser mealworm oils. The oils extracted by ethanol/isopropanol and ethanol/n-hexane significantly showed the highest extraction yield and efficiency, lightness, accelerated thermal stability, phenolics, tocopherols, vitamin D, campesterol, β-sitosterol, phosphatidylinositol and phosphatic acid, linoleic acid, and hypocholesterolemic/hypercholesterolemic ratio, while these organic mixtures meaningfully extracted lipids with the lowest peroxide value, free fatty acid, and atherogenicity and thrombogenicity indices. These solvents compared to pure ones could dissolve membrane and internal lipids with the complete disintegration of external structures. The ethanol/isopropanol mixture would be a promising solvent for n-hexane substitution to extract this oil on an industrial scale.

Quantitative and Qualitative Assessment of European Catfish (Silurus glanis) Flesh

Quantitative and qualitative flesh production in the Silurus glanis species was comparatively studied between two fish groups: one from aquaculture (AG) and the other from a natural environment, the Prut River (RG). Morphometry was carried out on the fish, and then biometric and conformational indices were calculated. Better values were found in the aquaculture catfish. The Fulton coefficient was 0.82 in the Prut River fish and 0.91% in the farmed ones. The fleshy index reached 19.58% in the AG fish and 20.79% in the RG fish, suggesting better productive capabilities in the AG fish. Postslaughter, the flesh yield and its quality were assessed at different moments throughout the refrigeration period (0–15 days), and chemical compound loss occurred. In the AG samples, the water content decreased by 8.87%, proteins by 27.66%, and lipids by 29.58%. For the RG samples, the loss reached 8.59% in water, 25.16% in proteins, and 29%in lipids. By studying the fatty acids profile and sanogenic indices, good levels of PUFA (31–35%) were found, and the atherogenic index reached 0.35–0.41 while the thrombogenic index ranged between 0.22 and 0.27. Consequently, it can be stated that fish origin and especially the refrigeration period influence the flesh proximate composition and nutritional value of European catfish.

Chemical and nutritional characterization of Ambelania duckei (Apocynaceae) an unexplored fruit from the Amazon region

Ambelania duckei Markgr is a species of the Apocynaceae family, native to the Amazon region that is unexplored from a nutritional point of view and studied in relation to its chemical constituents. This work presents an unprecedented study of the proximate composition, lipid profile, a chromatographic analysis, and the antioxidant activity of extracts obtained from the pulp, peel and seeds of the fruit. The results showed that potassium, calcium, and magnesium stood out as the most abundant key minerals in the fruit peel and pulp, with an emphasis on the potassium present in the fruit pulp at 1750.0 mg/100 g. The peel had the highest content of total phenolics (374.86 mg/g), flavonoids (15.54 mg/g), tannins (27.45 mg/g) and O-diphenols (379.36 mg/g; 645.71 mg/g). The antioxidant activity (AA) was highest in the peel compared to the pulp in the DPPH, ABTS, and ORAC tests showing: IC50 of 29.82; 43.67; and 407.13 µg/mL, respectively but a lower activity for the Fe2+ chelator. The analysis of the lipid fractions from the peel, pulp, and seeds of the A. duckei fruit resulted in 14 types of fatty acids. The major fatty acids found in the three parts of the fruit were oleic acid (peel, 22.52 %), palmitic acid (pulp, 17.34 %), and linoleic acid (seeds, 47.99 %). The lipid profile and nutritional aspects had a PUFA/SFA ratio (0.4–1.8) in the different parts of the A. duckei fruit; the atherogenic and thrombogenic indexes were higher in the peel (1.23) and pulp (0.62), respectively. The ratio between the hypocholesterolemic and hypercholesterolemic fatty acids (0.5 – 3.8) calculated for the fruit are within the desirable range for a nutritious food. The chromatographic analysis of the volatile organic compounds (VOCs) from the peel and pulp of the fruit, identified 74 VOCs, of which 60.9 % are related to terpenes, and emit notes such as cucumber, green, fatty, floral, and mint, due to the presence of substances with OAVs > 10, especially α-ionone, 1,8-cineole, 2,4-decadienal, and dodecanal. The analysis of the MS and MS/MS spectra of the chromatograms obtained by LC- QTOF-HRMS led to the identification of 26 compounds in the peel, seeds and pulp of A. duckei, such as fatty acids, phenolic acid, flavonoids, proanthocyanidins, alkaloids, and terpenoids. The results show that the pulp of A. duckei has potential as nourishing food and the nutritional and chemical aspects of the peel can be applied to commercial applications.

Cheese and Butter as a Source of Health-Promoting Fatty Acids in the Human Diet.

The assessment of fatty acid composition, including the content of conjugated linoleic acid cis9trans11 C18:2 (CLA) and trans C18:1 and C18:2 isomers in fat extracted from selected high-fat dairy products commonly available to consumers in retail sale on the Polish market, and a comparison of their indicators as to the quality of lipids was the aim of the study. The experimental materials were hard cheeses, white-mold cheeses, blue-veined cheeses, and butters. The conducted study demonstrated that various contents of groups of fatty acids and the values of lipid quality indices were found in the tested products. Butters turned out to be richer sources of short-chain, branched-chain, and odd-chain fatty acids. The fat extracted from butters and white-mold cheeses had a significantly higher (p < 0.05) content of n-3 fatty acids. Lower values of the n-6/n-3 ratio were determined in the fat extracted from butters and white-mold cheeses. The highest values of the thrombogenicity index (TI) were found in fat extracted from hard cheeses. Significantly lower values (p < 0.05) of the atherogenicity index (AI) and values of the H/H ratio were found in fat from mold cheeses. Fat from butters and white-mold cheeses had a significantly higher (p < 0.05) content of CLA and total content of trans C18:1.

The effect of Spirulina supplementation in ewes’ oxidative status and milk quality

Spirulina (SP) is an edible blue-green algae rich in bioactive compounds such as vitamins, minerals, antioxidants, and γ-linolenic acid. However, its impact on the oxidative status of ewes’ organism and milk, as well as on milk’s quality has not been extensively studied. Forty-eight dairy Chios ewes were divided into four homogenous groups (n = 12) and were fed individually. The concentrate of the control group (CON) had no SP, while in the concentrates of the treated groups, SP was added to obtain a daily supply of 5 (SP5), 10 (SP10), and 15 (SP15) g per animal. The milk yield and chemical composition were not affected by the addition of SP. Τhe proportion of short-chain fatty acids was increased in the milk of SP5 and SP10 ewes while those of medium-chain fatty acids were reduced in the milk of SP10 compared with the CON animals. The SP addition enhanced the proportion of polyunsaturated fatty acids and reduced the thrombogenicity index in milk, while its highest level tended to decrease the milk’s atherogenicity index. A rise in the activity of superoxide dismutase (SOD) in the blood plasma of SP-fed ewes was found. The same was observed for the activities of catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione transferase in the blood plasma of SP10, SP15, and SP5 fed ewes respectively. On the contrary, the protein carbonyls content (PC) in ewes’ s blood plasma declined by the dietary supplementation of SP. The oxidative stability of ewes’ milk improved by the dietary addition of SP as indicated by the rise in the activities of SOD, CAT, and GSH-Px and the total antioxidant capacity (measured by FRAP and ABTS assays) as well. Finally, the highest level of SP caused the sharpest drop in PC content of milk. In conclusion, the dietary supplementation with 15 g SP/ewe/day showed the potential to improve the ewes’ oxidative status of their organism and milk.

Fatty acid composition on diet and carcasses, growth, body indices and profile serum of Asian redtail catfish (Hemibagrus nemurus) fed a diet containing different levels of EPA and DHA

Background: The Asian redtail catfish Hemibagrus nemurus is a promising commercial aquaculture freshwater big-sized Bagridae catfish across Asian countries such as the Mekong, Malay Peninsula, and Indonesia. This study analysed the effect of eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3) supplementation in diets on changes in fatty acid compositions in feed and fish meat, lipid quality (atherogenic index and thrombogenic index), growth rate, body indicators, and serum metabolites of Hemibagrus nemurus juveniles. Methods: A total of 180 Asian redtail catfish (initial weight 54.80 ± 2.72 g) were fed four levels (0, 3,150, 6,300, and 9,450 mg of EPA+DHA/kg feed) sourced from fish oil. Diets were fed in triplicate in freshwater tarpaulin ponds, with 15 fish per tarpaulin pond. During the experiment, fish were fed 3% per day of the biomass weight. Results: Categorically, there were significant differences in the composition of fatty acids in the feed and fish meat. The atherogenic index was between 1.76 and 1.84, and the thrombogenic index was between 0.81 and 0.89 in all fish meat. Growth performance was significantly different between diets, while body indices did not make a significant difference between diets. The fish meat EPA and DHA showed positive linear relationships with diet EPA (p <0.001, r2 = 90%) and DHA diet (p<0.001, r2 = 85%). Serum metabolites among treatments D2 and D3 diet-fed feed for 60 days did not significantly differ. Glucose (GLU) levels had moderate relationships with triglycerides (TAG) (r2 = 65%), and GLU levels strongly correlated with low-density lipoprotein cholesterol (LDL-C) (r2 = 81%). Conclusions: Based on diets and whole-body carcass compositions, growth performance, and serum metabolites, Asian redtail catfish fed a diet containing 6,300 mg of EPA+DHA/kg feed are best for food safety.

Qualitative and Nutritional Evaluation of Paddlefish (Polyodon spathula) Meat Production

Polyodon spathula is a valuable species of sturgeon native to North America that has acclimatized very well in Europe. Detailed knowledge of the quantitative and qualitative productive performance of paddlefish meat is of interest. Through this article, we aimed to highlight the chemical composition, cholesterol, and collagen content of fillets issued from paddlefish aged two and three summers and to highlight, as well as the nutritional value, the profile of fatty acids and amino acids, the sanogenic indices and the biological value of proteins for the epaxial and hypaxial muscle groups. The chemical analysis of the fillets by age indicated slightly higher values in summer three, compared to summer two: +5.32% dry matter, +0.89% protein, +41.21% fat, therefore +10.94% gross energy and for collagen by 2.94%; instead, for water, minerals and the W/P ratio the values were lower by 1.52%, 10.08%, and 2.29%. The nutritional assessment revealed that paddlefish has a meat with high PUFA content (approx. 22% of total fatty acids) and good values of sanogenic indices (Polyunsaturation Index = 7.01–8.77; Atherogenic Index = 0.57; Thrombogenic Index = 0.38–0.39; Hypocholesterolemic Fatty Acids = 33.01–41.34; Hypocholesteromic/Hypercolesteromic Fatty Acids ratio = 1.9). Also, the proteins of these fish are of good quality for young and adult consumers (EAA index = 156.11; Biological Value = 158.46; Nutritional Index (%) = 28.30) and good enough for children (Essential Amino Acids Index = 96.41; Biological Value = 93.39; Nutritional Index (%) = 17.45).

Effects of Dietary Omega-3 Enrichment on the Chemical Composition and the Pathogenic Microbiota of Ovine Milk.

The demand for ovine milk and ovine dairy products is constantly increasing due to their exceptional sensorial characteristics and their health benefits for consumers. However, dairy fat content and composition are of particular concern for consumers as well as the medical community, as there are risk factors for coronary disease, diabetes mellitus, cancer, and other serious diseases. For this reason, attempts have been made to control/regulate the fat composition of ovine milk by modifying sheep dietary intake of polyunsaturated fatty acids. In this experimental trial, a group of sheep were fed for 30 days a diet enriched in flaxseeds and lupines, feed ingredients rich in omega-3 fatty acids, aiming to investigate the effects on fat composition and the microbiota of ovine milk. Chemical analysis of the collected milk showed that the omega-3 and omega-6 content was increased. On the opposite, the atherogenic and thrombogenic indexes decreased. Of importance was the semi-protective effect on the udder by the increased omega-3 dietary intake, as depicted by its impact on the biodiversity of the pathogenic microbiota. These findings suggest that ovine milk could be modified under specific conditions to be more appropriate for the consumption by people belonging to high-risk groups for various diseases.

Effects of supplementing sweet sorghum with grapeseeds on carcass parameters, and meat quality, amino acid, and fatty acid composition of lambs.

Sweet sorghum is an important forage crop for ruminants, especially in low rainfall areas. Grapeseeds are an abundant by-product of wine-making and contain bioactive substances that can improve the antioxidant capacity of meat. We examined the effect of sweet sorghum forage with supplementary grapeseeds on carcass and meat quality in lambs. Twenty-eight Small-tailed Han lambs (body weight = 19.1±1.20 kg), aged 3 to 4 months, were penned, and fed individually. The lambs were divided into four groups (n = 7 each) and were offered one of four diets: i) sweet sorghum silage; ii) sweet sorghum silage + grapeseeds; iii) sweet sorghum hay; and iv) sweet sorghum hay + grapeseeds. The grapeseeds were added to the concentrate at 6% DM and the diets were fed for 100 d. Sweet sorghum silage tended (p = 0.068) to increase hot carcass weight, while grapeseeds tended (p = 0.081) to decrease dressing percentage without affecting other carcass parameters. Lambs consuming supplementary grapeseeds increased (p<0.05) meat redness and tended to decrease (p = 0.075) concentration of methionine in meat. Lambs consuming sweet sorghum silage increased (p<0.001) water content of the meat and had a lower (p<0.05) concentration of n-6 polyunsaturated fatty acids (PUFA) and n-6:n-3 PUFA ratio than lambs consuming sweet sorghum hay. Saturated fatty acids content in meat was lowest (p<0.05) in lambs consuming sweet sorghum silage with grapeseeds. Lambs with supplementary grapeseeds tended (p<0.10) to increase eicosapentaenoic acid and docosahexaenoic acid and have a lower thrombogenic index than lambs not consuming grapeseeds. It was concluded that sweet sorghum with supplementary grapeseeds fed to lambs; i) improved the color of the meat to be more appetizing to the consumer; ii) tended to improve the fatty acids composition of the meat; and iii) lowered thrombogenic index of the meat.

Fatty acid profile, omegas, and lipid quality in commercial cuts of tambaqui (Colossoma macropomum Cuvier, 1818) cultivated in excavated tanks

This study aimed to determine the fatty acid profile, omegas, and lipid quality indices in commercial cuts of tambaqui (Colossoma macropomum). Samples were collected from two fish processing industries located in Rondônia state, Brazil. The experimental design was completely randomized, with processing performed in triplicate. Data were submitted for analysis of variance (ANOVA) to assess differences between commercial cuts. If ANOVA was statistically significant (α = 0.05), the averages were compared using Tukey's test. In the composition of fatty acids, there was a difference (p &lt; 0.05) between commercial cuts. The commercial cuts with the highest percentages of saturated fatty acids (SFAs) steak at 47.050%, monounsaturated fatty acids (MUFAs) fillet at 45.120%, and polyunsaturated fatty acids (PUFAs) band at 19.050%. In addition, the band expresses the highest values of omegas 3, 6, 7, and n-9. The indices prescribed ∑PUFAs/∑SFAs, ∑PUFAs (n-6/∑n-3), atherogenicity index, thrombogenicity index, and ratio between hypocholesterolemic and hypercholesterolemic fatty acids, indicating that commercial cuts have lipid quality. Nutritional information is important for conservation and processing processes, the development of new products on the market, and guidance on the form of preparation, thus providing commercial security for different market niches.

Evaluation of fatty acid-related nutritional quality indices in processed and raw (Lethrinus lentjan) fish fillets.

The Lethrinus lentjan fish is economically and nutritionally important in Iran. Therefore, knowledge of their chemical composition can help in the development of functional foods. In the present study, proximate and fatty acid compositions were determined in fresh and marinated Lethrinus lentjan fish. Fatty acids were identified by gas chromatography. The 24 pieces of fresh L. lentjan fish with an average weight of 470 ± 125 g were prepared. After removing wastes, the fish fillet was placed in a dish containing acetic acid, salt, garlic, and red pepper. The marinade product contained n-6PUFA (7.94%) and n-3PUFA (3.46%). The results showed that moisture content in the marinade sample decreased, while fat, protein, and ash contents increased and carbohydrate content was also decreased compared to the raw sample. The marinating process increased PUFA percentage (19.32%) and ratio of PUFA and SFA (0.61). The fresh and processed samples showed superior nutritional quality and the lowest h/H ratio, but AI and TI ratios were relatively favorable (p < .05). The thrombogenicity index (TI) (0.46) and atherogenicity index (AI) (3.56) were more than the recommended in terms of risk of coronary heart disease. It can be concluded that fresh and processed fish samples represent an excellent source of high-quality fat, demonstrating that this fish species' freshwater can be considered optimal for human consumption.

Summer Buffalo Milk Produced in China: A Desirable Diet Enriched in Polyunsaturated Fatty Acids and Amino Acids.

The objective of the study was to compare and reveal differences in basic chemical parameters, fatty acids, amino acids, and lipid quality indices of crossbred buffalo (swamp x river type) milk produced in summer and winter. The buffalo milk samples were collected in summer (Jul-Aug) and winter (Dec-Jan) from Hubei province, China. The samples were detected by using CombiFoss apparatus, gas chromatography, and an automated specialized amino acid analyzer. The results showed that the basic chemical parameters, fatty acid profiles, lipid quality indices, and amino acid profiles of crossbred buffalo milk differed between summer and winter. Specifically, summer buffalo milk exhibited a higher content of MUFA (monounsaturated fatty acids) and PUFA (polyunsaturated fatty acids) than winter buffalo milk. Summer buffalo milk had a lower content of major SFA (saturated fatty acids), a higher content of ω-3 and DFA (hypocholesterolemic fatty acids), a lower ω-6/ω-3 ratio, a higher value of 3 unsaturated fatty acid indices (C14, C16, C18), and a lower value of IA (index of atherogenicity) and IT (index of thrombogenicity) than winter buffalo milk. Additionally, 17 amino acids, including 8 EAA (essential amino acids) and 9 NEAA (non-essential amino acids) were higher in summer buffalo milk. These results indicated that summer buffalo milk was more health-beneficial than winter buffalo milk. Therefore, summer buffalo milk might be a desirable diet option for human nutrition and health. Our findings provide valuable information for the research and development of buffalo dairy products in China or other Asian countries.

Assessment of the chemical characteristics and nutritional quality of meat from broiler chicken fed black soldier fly (Hermetia illucens) larvae meal

In this study, we aim to evaluate the chemical characteristics, and nutritional quality of raw meat from broiler chicken fed a black soldier fly larvae meal. Three hundred twenty female broiler chicks were divided into four dietary treatments (8 replicates/treatments). Birds were fed maggot meal at 0%, 4%, 8%, and 12%. At 42 days of age, 16 birds/treatment were randomly selected and slaughtered. The chemical characteristics and nutritional quality of the meat were assessed. Abdominal fat, intramuscular lipid levels, and cholesterol levels increased (p < 0.05) in chicken-fed larvae meal. The latter had a higher level of mono-unsaturated and polyunsaturated fatty acids but a lower level of omega 3 (ω3) compared to the control. The atherogenicity index (AI) and the thrombogenicity index (TI) decreased with maggot meal incorporation. The unsaturation index was better at 4% and 8% maggot meal. Chickens fed with 8% maggot meal would present less risk for cardiovascular health.

Fatty acid composition in common deep-water shrimps of the Arabian Sea and its importance to human nutrition

AbstractDeep-water shrimps are recognized as non-conventional culinary delicacies owing to the presence of high-quality polyunsaturated fatty acids, even though they remain one of the predominantly unexplored marine fishery resources. In this study, variation of fatty acid profiles of Aristeus alcocki, Solenocera choprai, Heterocarpus chani and Plesionika quasigrandis, caught along the south-west coast of India (Arabian Sea) during the months spanning from September 2018–April 2019, were compared. Among the deep-water shrimps studied, A. alcocki contained a greater amount of n-3 fatty acids, such as docosahexaenoic and eicosapentaenoic acid along with optimal n-3/n-6 polyunsaturated fatty acid ratios beneficial for human nutrition (up to 8 during the winter months) when compared with other shrimps. In general, fatty acid concentrations exhibited variations, particularly with regard to polyunsaturated fatty acids, such as eicosapentaenoic acid and docosahexaenoic acid. These n-3 fatty acids were predominant during December–February of the studied year, but concentrations were noticeably lower during March and April. Females had considerably higher (P &lt; 0.05) C20–22n-3 fatty acid concentrations along with nutritionally balanced polyunsaturated/saturated fatty acid ratio than males. Results of the two-way multivariate analysis of variance (MANOVA) revealed statistically significant differences in the fatty acid profiles between the species and months. The interaction effects of months with species were also highly significant (Wilk's lambda = 0.000001; F = 187.7, P &lt; 0.0001). Lesser atherogenicity (&lt;3) and thrombogenicity (&lt;0.5) indices coupled with considerably greater n-3/n-6 fatty acid ratios recognized these deep-water species as a possible source of highly nutritional human food.

Hydrogelled emulsion from linseed oil and pea protein as a strategy to produce healthier pork burgers with high technological and sensory quality

Hydrogelled emulsions (HEs) produced with linseed oil and different levels of pea protein (PP) (0, 5, 10, 15, and 20%) were used to replace 50% of animal fat in burgers. The effect of this lipid reformulation on the nutritional, technological, oxidative, microbiological, and sensory quality of the burgers was evaluated during their refrigerated storage (4 °C for 12 days). The reformulated burgers displayed a reduction of >40% in fat and an increase of up to 10% in protein content. Lipid reformulation also increased the PUFA/SFA ratio and reduced the n-6/n-3 PUFAs ratio and the atherogenicity and thrombogenicity indices of the lipid fraction of the burgers. Including 5 and 10% of PP in the HEs made it possible to obtain burgers of high technological quality and with a sensory profile similar to full-fat products. PP was also efficient in reducing the increase in the lipid oxidation caused by the enrichment with n-3 PUFAs.

Chemical compositions, fatty acid profiles and selected contaminants in commercial potato and corn chips sold in the Tunisian market

In the last decades, the snack food market is experiencing an important expansion due to the new fast-paced life-style associated with modernization. Crispy snacks, or chips are the most popular snack around the world, nevertheless, their overconsumption is related to the incidence of many diseases. Subsequently, this work aimed to study the chemical composition of 13 brands of potato and corn chips available in the Tunisian market. The investigation was based on: the determination of their chemical, mineral, and fatty acid composition; an evaluation of their lipid quality indices; and a chromatographic analysis of pesticides, aromatic hydrocarbons and acrylamide content. The results showed that the chips samples were of a high carbohydrate and fat content of up to 64.54% and 42.98%, respectively, versus a low protein and mineral composition. The fatty acid profiling showed that saturated fatty acids represent more than 39% for the majority of samples. A poor lipid quality was also observed through their low unsaturated fatty acids /saturated Fatty Acids ratios, with a mean value of 2.24 and their high atherogenic and thrombogenic indices that reached 1.69 and 2.23 respectively. While the analysis of pesticide residues and polycyclic aromatic hydrocarbons showed that all compounds were below the maximum allowed levels, the acrylamide analysis highlighted contamination in all the samples tested, with some values exceeding the allowed threshold. In conclusion, it can be suggested that the crisps sold in the Tunisian market are of a poor nutritional quality and they are potentially unsafe for human consumption.

Oxidative stability and health-related indices of anhydrous milk fat and vegetable oil blends

Anhydrous milk fat (AMF), although relatively resistant to oxidation due to its low content of polyunsaturated fatty acids (FAs), raises certain health concerns due to a high saturated FAs content. Blending AMF with other edible fats is an ideal strategy for modifying its FA profile, health-promoting properties and oxidative stability. Addition of 30% vegetable oils to AMF significantly modified its FA profile. Oils with a high unsaturated FAs content, i.e., walnut, grapeseed and linseed oils, were particularly effective in increasing the content of unsaturated FAs in AMF and reducing its atherogenicity index and thrombogenicity index. AMF and vegetable oil blends were characterised by higher oxidative stability than pure vegetable oils. Blends comprising AMF and oils abundant in monounsaturated FAs, i.e., hazelnut, rapeseed and peanut oils, were most resistant to oxidation. Depending on intended use, the properties of AMF can be easily and cheaply modified through blending with selected vegetable oils.

Age-related alterations in the nutritional quality of Arctic grayling (Thymallus arcticus).

The biochemical composition of the muscle in Arctic graylings at three age stages was evaluated and compared. For conventional biochemical composition, there was no significant difference in the crude protein content among the three age stages, but the crude fat content in the 1 year old was significantly lower than that of the others (P<0.05). For amino acids composition, there was no significant difference in the content of essential amino acids and delicious amino acids among the three age stages, but the essential amino acid index of the 2 year old was the highest (72.205), indicating that its essential amino acid composition was more suitable for human needs. For fatty acids composition, the content of monounsaturated fatty acids (2.759mg/g) and polyunsaturated fatty acids (3.422mg/g) of the 3 year old was significantly higher than that of the others (P<0.05), and it also had relatively lowest atherogenicity index (0.419) and thrombogenicity index (0.179) and the highest hypocholesterolemic/hypercholesterolemic fatty acid ratio (2.404), indicating that its fatty acid composition was healthier and more beneficial to the prevention of human cardiovascular and cerebrovascular diseases. For minerals composition, the contents of iron, zinc, and copper increased with age, while the contents of selenium and manganese decreased with age, with the highest selenium content in the 1 year old (1.522mg/kg). The chromium, arsenic, lead, and cadmium contents were all lower than the Chinese national standard. In conclusion, the nutritional composition of Arctic graylings variated with age and consumers could select suitable Arctic graylings based on their nutritional needs. PRACTICAL APPLICATION: This work analyzed the nutritional components in the muscle of Arctic graylings at different ages, which can provide an effective reference for consumers when purchasing Arctic graylings.

Fatty Acid and Tocopherol Composition of Pomace and Seed Oil from Five Grape Varieties Southern Spain.

Grape pomace and seeds are important winemaking by-products. Their oils are rich in bioactive compounds such as fatty acids and tocopherols. We have characterized oils from both by-products from five Spanish grape varieties (Palomino Fino, Pedro Ximénez, Muscat of Alexandria, Tempranillo and Tintilla de Rota). A high content of UFAs was found in all the analyzed samples. Grape pomace oils generally had the same oleic acid (PUFAω-6) content as seed oils, and lower PUFA contents; they also had a markedly higher linolenic acid (PUFAω-3) content, improving the PUFAω-6/PUFAω-3 ratio. All the oil studied show good indicators of nutritional quality: low values of the atherogenicity (0.112–0.157 for pomace, 0.097–0.112 for seed) and thrombogenicity indices (0.30–0.35 for pomace, 0.28–0.31 for seed) and high values of the relationship between hypo- and hypercholesterolemic fatty acids (6.93–9.45 for pomace, 9.11–10.54 for seed). Three tocopherols were determined: α-, γ- and δ-tocopherol. Pomace oils have higher relative contents of α- and δ-tocopherol, whereas seed oils have higher relative contents of γ-tocopherol. A significantly higher content of total tocopherols has been found in pomace oil; it is higher in the oils from red varieties of pomace (628.2 and 706.6 mg/kg by-product), and in the oils from pomace containing stems (1686.4 mg/kg by-product). All the oils obtained can be considered as a source of vitamin E, and their consumption is beneficial for health.