This paper proposes a three-dimensional failure criterion model for unidirectional fiber-reinforced composites. In contrast to previous models that only accounted for the effect of fiber misalignment in the presence of longitudinal compressive stress, the proposed failure criterion model comprehensively considers the effect of localized misaligned regions on the failure under any stress state. Another key contribution of this study is the introduction of the effective misalignment angle. Considering effective misalignment angles, the proposed failure criterion model can reasonably reveal the effect of localized misaligned regions on the failure behavior under different stress states. The agreement between the predicted results and the experimental data proves that the proposed model has good applicability. Furthermore, the influence of initial misalignment angles on failure is analyzed under varying stress conditions. The results indicate that even under longitudinal tensile stress, the initial misalignment angle still plays an important role in the failure behavior of materials.