Thirteenth Day Research Articles

Photobiomodulation Modulates Proliferation and Gene Expression Related to Calcium Signaling in Human Osteoblast Cells.

Introduction: Photobiomodulation with low-level laser treatment can enhance bone formation by stimulating the cell division of osteoblasts and increasing the amount of protein deposition, thus encouraging the formation of new bone. The aim of this study was to evaluate the effects of photobiomodulation with a low-level laser on proliferation and gene expression related to calcium signaling in human osteoblasts. Methods: Osteoblastic cell lines of the hFOB1.19 lineage, human osteoblasts, were grown and assigned into two groups, control (C; n=78 cultured wells) and photobiomodulation (L; n=78 cultured wells) with n=6 per day of the experimental period. Cells were cultured (immature at 34 ºC), and after maturation at 37 ºC, group L cells were exposed to laser irradiation with a low-level laser device (gallium and aluminum arsenide), at a wavelength of 808 nm, a power output of 200 mW, and a power density of 200 mW/cm2. The energy delivered to the cells was 37 J/cm2, with a beam area of 0.02 mm2 and an exposure time of 5 seconds. This treatment was applied daily for a period of 13 days. Following this, the number of cells was counted, and RNA was isolated, measured, and then converted into cDNA for further quantification using a comparative Ct method with real-time polymerase chain reaction. The results were then subjected to statistical analysis through a Mann-Whitney test, with a significance level of P<0.05. Results: The cell count in the L group (37.25x10±4±22.02) was statistically higher compared to the control group (22.75x10±4±7.660) with a P value of 0.0259. The values of 2-ΔΔCt for S100A6, plasma membrane calcium ATPase (PMCA), and calmodulin genes indicated hyper-expression on the thirteenth day, while the osteocalcin gene showed hypo-expression. Conclusion: The study suggests that the photobiomodulation mechanism with a low-level laser may regulate gene expression in human osteoblasts in a dose-dependent and cumulative manner.

The effect of noise reduction on work-related stress: a quasi-experimental study on weaving workers exposed to high levels of noise

Introduction: The textile industry is one industry in the world with a high risk in its production process. The operation of weaving machines generates high levels of noise, which can cause various adverse effects on workers' health, especially work-related stress. IT Co. Ltd., one of the largest textile companies in Solo, Central Java, since 1975, has not been able to overcome the problem of the high noise intensity, especially in the weaving production room. Methods: This study aims to determine the effect of reducing noise exposure on work-related stress by installing sound-absorbing materials from coconut fiber waste. The study was carried out in 2 phases. The first phase measured noise intensity and work stress before the intervention. The second phase was carried out for 12 measurements daily after installing sound-absorbing material from coconut fiber waste. Results: Installing sound-absorbing material from the coconut fiber waste on the second to the thirteenth day positively reduced the average noise intensity and work-related stress score compared to the first day before the intervention. Conclusion: The study showed a significant difference in the average noise intensity and work-related stress before and after installing the sound-absorbing materials on 13 days of measurement.

Comparisons of Rice Seed Growths Due to Alternating and Direct Current Electric and Magnetic Field Influences

It is commonly known that electric and magnetic fields of power transmission negatively and positively influence plants. Unfortunately, studies on these influences are minimal, particularly considering the comparison between magnetic field and electric field, on both DC (direct current) and AC (alternating current). This research aims to compare the influences of magnetic field and electric field, both on AC and DC, on rice plant growth. Firstly, prototypes, including the equipment, were constructed to generate AC and DC electric fields using parallel plates of a medium voltage transformer and Cockroft-Walton circuits. Meanwhile, the AC and DC magnetic fields were prepared using three different diameter current-injected coils. The rice seeds were exposed to electric and magnetic fields for one month, with plate distance and coil diameter variations. The results showed that the rice seeds grew differently according to the respective types and magnitudes of the fields. In the first two days, the rice seed growths exposed to electric and magnetic fields were higher than those without field exposures. However, since the thirteenth day, the rice growth rate with field exposure was lower than without. This study also shows that the influences of the DC electric and magnetic fields were more potent than the AC fields. The averages of rice seed growth decreasing rate for the AC and DC electric fields and AC and DC magnetic fields were 0.00827 cm/(kV/m), 0.01167 cm/(kV/m), -0.13267 cm/mT and 1.99005 cm/mT, respectively. As a general suggestion in sites, rice plants should be avoided from a transmission line due to high voltage direct current (DC) rather than that alternating current (AC).

Effect of Alcoholic Extract of the Plant Conyza bonariensis on the Parasite Hymenolepis nana in Albino Mice Infected with it Experimentally

This study was conducted to investigate the effect of an alcoholic extract of the Mountain grass plant Conyza bonariensis on adults of the dwarf tapeworm Hymenolepis nana in albino mice infected with it experimentally, where Albino mice of the strain Balb/C were infected with these worms experimentally, it was divided into 5 groups, each group consisting of 6 rats, where the first group (control) was given distilled water only. As for the other groups, they were dosed with different concentrations of the alcoholic extract of the plant 50, 100, and 200 mg/kg, The last group was given 15 mg/kg of Niclosamide in one dose per day for 13 days. The results showed the effective effect of the concentrations of the alcoholic extract and the drug Niclosamide in the treatment of albino mice, and it produced a high therapeutic efficiency of 100%, with a variation in the period required for complete treatment and complete elimination of helminths, where the highest concentration of 200 mg/kg and the use of the drug led to a complete recovery of the infected Albino mice during the ninth day of treatment, and it was noted that the worm eggs disappeared from the swabs of the treated mice, while the infection continued in the control group until the thirteenth day, then died after that.

Evaluation of the Efficiency of some Integrated Control Agents in Controlling the Different Stage of the Bemisia tabaci (Homoptera: Aleurodidae) in the Field

The cotton whitefly is a complex biological species. Bemisia tabaci (Genn) is an important pest that affects various crops in Iraq and the world, which causes economic damage to the vegetable production of crops. For this reason, the study aimed to use some elements of integrated control to whitefly in all its harmful stages on crops and to evaluate the efficiency of biopesticides (Bacillus bassiana, Beauveria bassiana, Paecilomyces lilacinus fungus, Entomopathogenic nematodes for both sexes, Heterohabditis sp. - Steinernema sp.)and chemical pesticides (Alafsket, Oxymatrin, Baron) used in the field against the different stages of the insect, as these bio and chemical treatments recorded a high mortality rate for eggs and primary stages that were more sensitive than pupae and adults who had a lower death rate for those advanced stages of the insect. Where the pathogenic nematodes gave the highest mortality rate compared to the used biopesticides, the results of the nematode suspension for the egg death experiment on the third day of treatment were 84.0%. In comparison, the treatment results for the experiment with the destruction of primary nymphs for the seventh day were 80.3%. The results of the treatment for the adult mortality experiment on the thirteenth day were 87.50% (note that nematodes were used in the field under suitable conditions for the growth of effective larvae to control against the stages of the insect, and it was 25-30 °C and humidity 60, and the diluted nematode suspension was kept under 10 °C to maintain the vitality of the effective larvae. As for the chemical treatments, the chemical pesticide Offset was superior to a high mortality rate for different insect stages compared to other chemical treatments. As for the experiment results, the morality of the primary nymphs on the seventh day was 87.7%, and the pesticide treatment for the experiment with the death of adults on the thirteenth day was recorded at 94.17%.

The Effect of Combined Administration of Sea Urchin Gonad and Aloe Vera Suspension on the Enhancement of Macrophage Phagocytosis Activity in Male White Rats Induced by Salmonella Typhimurium

This research determined the influence of the administration of combined gonadal sea urchin suspension and aloe vera to improve the macrophage phagocyte activities of male mice (Rattus norvergicus). This experimental laboratory research used a posttest-only control group design with white mice (Rattus norvegicus). The researcher grouped the mice into three groups: the negative control group with CMC-Na (0,5% /kg Weight); the positive group with Levamisole 0,9 mg/ 200 weight; and the intervention group with combined gonadal sea urchin suspension, Tripeneustes gratila, with a dose of 2,2700g/kg Weight, and the extracted aloe vera for 15ml. The researcher provided the treatment for 12 days, orally, twice a day with the induction of Salmonella typhimurium of 104 CFU 0,1 ml on the fifth day. The outcome of the macrophage phagocyte capability on the thirteenth day was calculated with the phagocytes index. The One-Way Anova test of gonadal sea urchin suspension, Tripneustes gratila with a dose of 2.2700 g/kg Weight with the extracted aloe vera indicated the highest phagocytes index, 2.28 (p&lt;0.05) than the control group. The combined gonadal sea urchin suspension, Tripneustes gratilla, with the extracted aloe vera could improve the macrophage phagocyte activities.

A 42-Year-Old Man With Postoperative Fever After Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy

&lt;p&gt;肝癌為國人不可忽視的重要健康議題，其治療方式包括手術治療及非手術治療。接受肝臟切除手術常見的併發症有肝衰竭、出血、急性腎損傷、膽汁滲漏及肋膜積液等，若介入治療時機適當，能減少嚴重併發症而降低生命危險，故術後的照護為重要的目標。本個案是一位42歲男性，為B型肝炎帶原，因診斷為巨大肝臟腫瘤接受兩階段肝切除手術，於第二階段術後第13天出現高燒、引流液呈黃褐色液，相關臆測診斷有腹內感染及肋膜積水等，一一排除後，經由腹部超音波及胸部X光檢查，確立有膽汁滲漏及肋膜積液等複雜性併發症，透過給予胸腔穿刺、腹部引流管置放及抗生素治療一週後病情穩定且併發症獲得控制，照護期間為2020年7月11日至2020年8月10日，期望藉此經驗能做為日後照護此類個案之參考。&lt;/p&gt; &lt;p&gt;&amp;nbsp;&lt;/p&gt;&lt;p&gt;Hepatocellular carcinoma is undoubtedly a significant health issue. The medical treatments of the dis&amp;shy;ease include operative and non-operative management. The most common complications after hepatic resection are liver failure, hemorrhage, acute kidney injuries, bile leakage, and pleural effusion. How&amp;shy;ever, the possibility of severe complications could be decreased if proper postoperative care is provid&amp;shy;ed. This can, meanwhile, reduce the threats to lives. Thus, careful postoperative care plays a vital role. The case in this study is a forty-two-year-old male hepatitis B carrier. He was diagnosed with giant hepatocellular carcinoma after clinical physical examinations and imaging tests and then received a two-stage hepatectomy. He had a postoperative fever on the thirteenth day after liver resection with brownish-yellow fluid from drainage. Therefore, postoperative intra-abdominal infection with pleural effusion were suspected. After receiving an abdominal ultrasound and chest X-ray, it was confirmed that he had complications of bile leakage and pleural effusion. His condition was well-controlled after a week under the treatment of thoracentesis, abdominal drainage as well as antibiotics. The period of postoperative care was provided from July 11 to August 10, 2020. This case could be a reference for taking care of patients with similar issues.&lt;/p&gt; &lt;p&gt;&amp;nbsp;&lt;/p&gt;

P2X7 receptor contributes to long-term neuroinflammation and cognitive impairment in sepsis-surviving mice.

Introduction: Sepsis is defined as a multifactorial debilitating condition with high risks of death. The intense inflammatory response causes deleterious effects on the brain, a condition called sepsis-associated encephalopathy. Neuroinflammation or pathogen recognition are able to stress cells, resulting in ATP (Adenosine Triphosphate) release and P2X7 receptor activation, which is abundantly expressed in the brain. The P2X7 receptor contributes to chronic neurodegenerative and neuroinflammatory diseases; however, its function in long-term neurological impairment caused by sepsis remains unclear. Therefore, we sought to evaluate the effects of P2X7 receptor activation in neuroinflammatory and behavioral changes in sepsis-surviving mice. Methods: Sepsis was induced in wild-type (WT), P2X7-/-, and BBG (Brilliant Blue G)-treated mice by cecal ligation and perforation (CLP). On the thirteenth day after the surgery, the cognitive function of mice was assessed using the novel recognition object and Water T-maze tests. Acetylcholinesterase (AChE) activity, microglial and astrocytic activation markers, and cytokine production were also evaluated. Results: Initially, we observed that both WT and P2X7-/- sepsis-surviving mice showed memory impairment 13 days after surgery, once they did not differentiate between novel and familiar objects. Both groups of animals presented increased AChE activity in the hippocampus and cerebral cortex. However, the absence of P2X7 prevented partly this increase in the cerebral cortex. Likewise, P2X7 absence decreased ionized calcium-binding protein 1 (Iba-1) and glial fibrillary acidic protein (GFAP) upregulation in the cerebral cortex of sepsis-surviving animals. There was an increase in GFAP protein levels in the cerebral cortex but not in the hippocampus of both WT and P2X7-/- sepsis-surviving animals. Pharmacological inhibition or genetic deletion of P2X7 receptor attenuated the production of Interleukin-1β (IL-1β), Tumor necrosis factor-α (TNF-α), and Interleukin-10 (IL-10). Conclusion: The modulation of the P2X7 receptor in sepsis-surviving animals may reduce neuroinflammation and prevent cognitive impairment due to sepsis-associated encephalopathy, being considered an important therapeutic target.

Antitumor effect of selenium-rich Brazil nuts and selenomethionine dietary supplementation on pre-existing 4T1 mammary tumor growth in mice.

Selenium (Se) is an essential micronutrient known to play an important role in the antioxidant system that can potentially influence tumor growth. We aimed to investigate the effects of dietary Se supplementation after detection of 4T1 mammary tumor growth in BALB/c mice. Thirty female mice received subcutaneous inoculation of 4T1 cells. After five days, all animals presenting palpable tumors were randomly assigned to three groups: a control group (Se-control) receiving a diet with adequate Se (0.15 mg/kg) and two other groups that received Se-supplemented diets (1.4 mg/kg of total Se) with either Brazilian nuts (Se-Nuts) or selenomethionine (SeMet). Data were assessed by either One or Two-way ANOVA followed by Tukey's HSD or Bonferroni's post hoc tests, respectively. Both Se-supplemented diets reduced tumor volume from the thirteenth day of feeding compared with the Se-adequate (control) diet (p < 0.05). The SeMet group presented a higher Se blood concentration (p < 0.05) than the Se-control group, with the Se-Nuts group presenting intermediate values. Selenoprotein P gene expression in the liver was higher in the Se-Nuts group than in the Se-control group (p < 0.05), while the SeMet group presented intermediate expression. Dietary Se supplementation, starting after detection of 4T1 palpable lesions, reduced tumor volume in mice.

ОЦЕНКА БЛИЖАЙШИХ И ОТДАЛЕННЫХ РЕЗУЛЬТАТОВ АКУШЕРСКОГО КРОВОТЕЧЕНИЯ С ЭМБОЛИЗАЦИЕЙ МАТОЧНЫХ АРТЕРИЙ И ПЕРЕНЕСЕННОЙ КОРОНАВИРУСНОЙ ИНФЕКЦИЕЙ (КЛИНИЧЕСКИЙ СЛУЧАЙ)

The study aims to present a complicated clinical case of a woman who almost died due to comorbid obstetric pathology associated with a coronavirus infection. The term “near miss” refers to the detailed analysis of each clinical case classified as a critical obstetric condition, as well as the registration of patients with such conditions in the Russian register established in 2021 to prevent maternal mortality and make management decisions. A severe clinical near miss case of a female patient included the following serious obstetric syndromes: postpartum obstetric hemorrhage followed by uterine artery embolization, endomyometritis by the third day, partial thrombosis of the branches of the right lower lobe pulmonary artery by the seventh day, thrombosis of parametrial vessels, partial necrosis of the uterus by the eighth day, and a new coronavirus infection COVID-19 by the thirteenth day after delivery. The complexity of diagnosis and treatment is demonstrated in the results of the study in the acute period and in the outcome of the disease.

Multiple individuals incubating and nest building, and winter breeding, in the Black-chinned Honeyeater Melithreptus gularis

The role of the male and female in the incubation of eggs is unknown in 30 of the 70 species of Australian honeyeaters, but in most of the remainder, only the female is thought to incubate. Nevertheless, incubation by both male and female is known to occur in six species, including the Brown-headed Honeyeater Melithreptus brevirostris. I observed and video-recorded activities at two nests of one group of four Black-chinned Honeyeaters M. gularis gularis in south-eastern Queensland. Nest attentiveness (incubation constancy) averaged 88% over three sample days. At least two birds participated in incubation, as evidenced by numerous change-overs, strongly suggesting that both male and female were involved. Incubation bouts averaged 7.2 minutes, but varied significantly over the 3 days, and off-bouts averaged 1.3 minutes but did not vary significantly. Nestling provisioning rates varied from 7.5 to 14.4 feeds h-1 on the fourth and thirteenth days after the estimated hatching day. Although cooperative breeding could not be confirmed, up to three birds were seen at this nest during the nestling stage, and up to three at the second nest before incubation began. Breeding records indicate that in south-eastern Queensland laying peaks in winter, beginning in late autumn, 2 months earlier than records suggest in New South Wales.

Therapeutic Comparison Between Alcoholic and Aqueous Plant Extract of Tannins with Metronidazole in Experimentally Infected Laboratory Mice Cryptosporidium parvum Oocysts

The current study was conducted during the period between from the beginning of October 2021 until the end of March 2022. The aim of the study was to measure the therapeutic efficacy of alcoholic and aqueous extracts from the local tannins Cupressus sempervirens for the treatment of experimental infected laboratory mice with Cryptosporidiosis.&#x0D; Through a significant decrease in the number of Oocyst of the parasite that causes infection Cryptosporidium parvum after oral administration of the aqueous and alcoholic extracts with three concentrations (2, 1.3, 1) mg/ml compared to MTZ drug, as the alcoholic extract proved its efficacy by stopping the shedding of parasite Oocyst at the ninth day of treatment with its total absence on the eleventh day of treatment, while the shedding of parasite Oocysts in the group treated with aqueous extract stopped on the eleventh day and completely absent on the thirteenth day of infection.

Protective Effect of Silymarin and Boric Acid Against Isoproterenol-Induced Myocardial Infarction in Mice

This study aimed to compare the protective or therapeutic effects of silymarin and/or boric acid against myocardial infarction induced by isoproterenol. Distilled water was given orally by gavage to the GI and GII groups for fourteen days. The GIII group was received silymarin (100mg/kg), while the GIV group was given boric acid (100mg/kg) for 14 days. In the last group, both silymarin and boric acid were given orally (gavage) for fourteen days. On the thirteenth and fourteenth days of the study, while isotonic water was applied to the GI group, isoproterenol (85mg/kg) was applied to the other groups subcutaneously. Plasma ALT, AST, Ca, CK, CHO, TP, Ing P, IL-6, IL-12, PAL-1, and sPLA2 parameters were evaluated. Histopathological examination was performed on the heart, lung, liver, kidney, spleen, cerebrum, and cerebellum tissues taken from mice. The CK level was found to be significantly lower in the GIII and GV groups compared to the GII group (P

Resveratrol modulates the gut microbiota of cholestasis in pregnant rats.

This study aims to investigate the effect of resveratrol on intrahepatic cholestasis of pregnancy (ICP) and its effect on the gut microbiome profiles, thus contributing to the potential therapeutic strategies for ICP. ICP rat models were established by injecting 17α-ethinylestradiol (EE) subcutaneously from the thirteenth day of gestation for four days and then treated with EE (D group, n=5), resveratrol (R group, n=5), or ursodeoxycholic acid (UDCA; U group, n=5) from the seventeenth to the twentieth day of gestation. Fecal samples were analyzed with 16S ribosomal RNA (rRNA) sequencing. In results: the gut microbiota of pregnant rats was characterized with reduced alpha diversity (Chao1 index), and significant variation in the microbiota structure (ANOSIM) was also observed after being treated with EE. The richness of four phyla and ten genera was upregulated, and five phyla and ten genera were downregulated by EE treatment. The dysbiosis of Bilophila, Ruminococcus, and Actinobacteria caused by EE treatment was reversed by resveratrol administration. There was a correlation between total bile acid and alanine aminotransferase in ICP rats. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis results suggested that the secondary bile acid biosynthesis was decreased, and the alanine, aspartate, and glutamate metabolism was increased after being treated with EE in pregnant rats. In conclusion, EE treatment could lead to gut microbiome dysbiosis and bile acid metabolism dysregulation in pregnant rats. Resveratrol could partially rescue gut microbiota dysbiosis and improve the biochemical characteristics caused by EE treatment.

In Vivo Vasospasm Induction by Ultrasound Application in the Chicken Chorioallantoic Membrane Model

Cerebral vasospasm is a highly investigated phenomenon in neurovascular research. Experimental vasospasm models are irreplaceable for the evaluation of new antivasospastic drugs. In this study, we assessed the reliability of in vivo vasospasm induction by ultrasound application in the chicken chorioallantoic membrane (CAM) model. After incubation of fertilized chicken eggs for four days, a fenestration was performed to enable examination of the CAM vessels. On the thirteenth day, continuous-wave ultrasound (3 MHz, 1 W/cm2) was applied on the CAM vessels for 60 s. The ultrasound effect on the vessels was recorded by life imaging (5-MP HD-microscope camera, Leica®). The induced vessel diameter changes were evaluated in a defined time interval of 20 min using a Fiji macro. The vessel diameter before and after sonication was measured and the relative diameter reduction was determined. A first reduction of vessel diameter was observed after three minutes with an average vessel-diameter decrease to 77%. The maximum reduction in vessel diameter was reached eight minutes after sonication with an average vessel diameter decrease to 57% (mean relative diameter reduction of 43%, range 44–61%), ANOVA, p = 0.0002. The vasospasm persisted for all 20 recorded minutes post induction. Vasospasm can be reliably induced by short application of 3 MHz-ultrasound to the CAM vessels. This might be a suitable in vivo model for the evaluation of drug effects on vasospasm in an experimental setting as intermediary in the transition process from in vitro to in vivo assessment using animal models.

POLISH LAW OF 12 FEBRUARY 2022 «ON ASSISTANCE TO UKRAINIAN CITIZENS IN CONNECTION WITH THE MILITARY CONFLICT ON THE TERRITORY OF THE STATE». PRELIMINARY ANALYSIS

POLISH LAW OF 12 FEBRUARY 2022 «ON ASSISTANCE TO UKRAINIAN CITIZENS IN CONNECTION WITH THE MILITARY CONFLICT ON THE TERRITORY OF THE STATE». PRELIMINARY ANALYSIS

Establishment of a cytokine release syndrome associated with chimeric antigen receptor T cell treatment in SCID/Beige mice model

Objective: To establish a cytokine release syndrome (CRS) mouse model related to CAR-T cell therapy and provide a research model for the clinical phenomena. Methods: CAR-T cells targeting human CD19 molecule were constructed by molecular cloning and lentiviral transfection. Flow cytometry (FACS) was used to detect the transfection efficiency of CAR-T cells. The tumor-killing efficiency of CAR-T cells was detected by ELISA and flow cytometry. The CAR-T cells were injected into the tumor-bearing SCID/Beige mice through tail vein, and divided into phosphate buffered solution (PBS) group, low-burden group (1×10(5) Raji-Luc2 cells) and high-burden group (5×10(5) Raji-Luc2 cells). The tumor treatment effect was detected by animal in vivo imaging. Serum levels of cytokines including human IFN-γ, human IL-2, mouse IL-6, and mouse GM-CSF were measured by ELISA. The health status of the mice was evaluated by pathological examination. Results: The health scores of T cell group and T cell+ OKT-3 group were (1.15±0.08) and (2.90±0.15), respectively, after the injection of human T cell and T cell + OKT-3 antibody through tail vein, and the difference was statistically significant (P<0.001). The serum levels of human IL-2, human IFN-γ, human IL-15, mouse IL-6 and mouse GM-CSF in T cell+ OKT-3 group were (1 064.00±50.14), (1 285.00±193.90), (202.4±18.76), (1 478.00±289.20) and (350.70±42.27) pg/ml, respectively, higher than (22.67±6.36), (23.67±3.71), (44.33±14.45), (147.30±36.20), (138.00±22.74) pg/ml in T cell group (P<0.05). OKT-3 combined with human T cells caused a rapid increase in serum levels of human IL-2, human IFN-γ, mouse IL-6 and mouse GM-CSF, accompanied by an increase in body temperature and weight loss. CD19-targeting CAR-T cells were successfully constructed, and the positive rate of CAR-T cells was >30% detected by flow cytometry. ELISA results showed that in the presence of CD19 antigen, IL-2 and IFN-γ secreted by CAR-T19 cells co-incubated with Raji and Nalm were (561.00±37.07), (680.30±71.27), (369±25.71) and (523.00±26.31) pg/ml, respectively, higher than (55.00±20.53) and (64.00±7.55) pg/ml in the co-incubated with K562 group (P<0.001). Activated CAR-T19 cells were reinjected through the tail vein on the seventh day after tumor formation. Imaging experiments in mice showed that on the thirteenth day after tumor formation, the fluorescence intensities of tumors in the low-burden and high-burden groups were lower than on the seventh day of tumor inoculation, and the fluorescence intensity of tumors in the high-burden group decreased from 144.00±24.69 to 5.02±2.35 (P=0.005). The fluorescence intensity of low burden group decreased from 58.47±9.36 to 3.48±1.67 (P=0.004). The serum levels of T cell activation related cytokines IL-2, IL-15 and IFN-γ increased rapidly, and the secretion of monocyte related cytokines IL-16 and GM-CSF increased, accompanied by the typical characteristics of CRS such as increased body temperature and weight loss at 72 hours after injection of CAR-T19 cells. Conclusions: CAR-T cells targeting CD19 molecule are successfully constructed, and CRS phenomenon is verified in tumor-bearing mice by CAR-T cell re-infusion, providing an animal model for the mechanism of CAR-T treatment-related CRS and CRS prevention strategies.

Clinical case of associative course of panleukopenia and intestinal yersiniosis in cats

The study of the course and manifestation of mixed infections is an important modern problem of both humane and veterinary medicine because their diagnosis is quite complex and time-consuming. The clinical manifestation of double and sometimes triple infection has its characteristics, which depend on various biotic and abiotic factors. To date, it has been proven that the associative course of several infectious diseases is registered more and more often, which confirms the case of simultaneous manifestation of panleukopenia and intestinal yersiniosis in domestic outbred cats. Diagnosis of mixed infection was comprehensive through general clinical and laboratory studies, the use of rapid test systems to confirm the diagnosis of panleukopenia and RNGA with specific yersinia antigens to determine the status of intestinal yersiniosis in cats. The latter was caused by serovar Y. enterocolitica O: 9 with a diagnostic titer of 1: 400. Thanks to microbiological studies, the causative agent of intestinal yersiniosis Y. enterocolitica was isolated. Its biological properties were determined, and antimicrobial sensitivity to enrofloxacin and doxycycline was established against the background of polyresistance to ceftriaxone and tetracycline. During treatment, it was found that the clinical case of intestinal yersiniosis was also due to the simultaneous infection of Y. enterocolitica with two types of serotypes O: 3 and O: 9. Which also have their own specific features. But in most cases, we have different sensitivity to antibacterial drugs, in contrast to the case we described. Clinical signs of the associated course of panleukopenia and intestinal yersiniosis in domestic outbred cats were characterized by prolonged diarrhea, streaks of blood and mucus, apathy, refusal to feed, general weakness. Therapeutic measures containing antibacterial drugs, energy sources, crystalloids, buffer infusions which were aimed at reducing dehydration and maintaining water-salt balance in the complex, were effective, clinical signs of the disease disappeared on the eighth day of treatment, and complete isolation of the pathogen in the environment ceased on the thirteenth day, which indicated the complete recovery of the animal. However, to prevent re-infection, it was recommended to treat disinfectants with care items and utensils. And also to avoid direct contact with animals that have pronounced clinical signs of disease. To prevent the occurrence of panleukopenia, scheduled mass vaccinations with mandatory observance of time limits.

Gamma-Decanolactone Alters the Expression of GluN2B, A1 Receptors, and COX-2 and Reduces DNA Damage in the PTZ-Induced Seizure Model After Subchronic Treatment in Mice.

Gamma-decanolactone (GD) has been shown to reduce epileptic behavior in different models, inflammatory decreasing, oxidative stress, and genotoxic parameters. This study assessed the GD effect on the pentylenetetrazole (PTZ) model after acute and subchronic treatment. We evaluated the expression of the inflammatory marker cyclooxygenase-2 (COX-2), GluN2B, a subunit of the NMDA glutamate receptor, adenosine A1 receptor, and GD genotoxicity and mutagenicity. Male and female mice were treated with GD (300mg/kg) for 12days. On the tenth day, they were tested in the Hot Plate test. On the thirteenth day, all animals received PTZ (90mg/kg), and epileptic behavior PTZ-induced was observed for 30min. Pregabalin (PGB) (30mg/kg) was used as a positive control. Samples of the hippocampus and blood were collected for Western Blotting analyses and Comet Assay and bone marrow to the Micronucleus test. Only the acute treatment of GD reduced the seizure occurrence and increased the latency to the first stage 3 seizures. Males treated with GD for 12days demonstrated a significant increase in the expression of the GluN2B receptor and a decrease in the COX-2 expression. Acute and subchronic treatment with GD and PGB reduced the DNA damage produced by PTZ in males and females. There is no increase in the micronucleus frequency in bone marrow after subchronic treatment. This study suggests that GD, after 12days, could not reduce PTZ-induced seizures, but it has been shown to protect against DNA damage, reduce COX-2 and increase GluN2B expression.

Solitary extramedullary plasmacytoma presenting as an adrenal tumor: case report and literature review

Solitary extramedullary plasmacytoma, an extremely rare case which accounts for about 3% of all plasma cell neoplasms, often occurs in the head and neck region such as para nasal sinuses, nasal cavity and oral cavity, it also occurs in the lymph nodes, lungs, thyroid, intestine, liver, pancreas, testis, breast, or skin. Isolated primary plasmacytoma of adrenal is extremely rare and only nine documented cases have been reported in the literature. We are presenting the 10th case which is the youngest patient until now with symptoms of fever and hepatosplenomegaly. A 19-year-old female was admitted with an irregular fever for 20 days. After a series of investigations were carried out there were no CRAB symptoms (hypercalcemia, renal failure, anemia and bone lesions), no free light chain and no more than 10% increase in plasma cell on bone marrow examination. Computed tomography (CT) scan revealed a tumor in the left adrenal region, and it was diagnosed to be a solitary extramedullary plasmacytoma on biopsy. She underwent the tumor resection one month after admission and recovered well after operation without fever and was discharged from hospital on the thirteenth post-operative day. She has been followed up for 5 years without any sign and symptom of tumor recurrence. Extramedullary plasmacytoma of adrenal gland in an extremely rare disease and usually diagnosed late in life but it can present in younger patients with variable symptoms. However, the surgical treatment yields excellent long-term results. So, complete surgical resection of the lesion is not only a good diagnostic measure, but also an intent-for-cure treatment for solitary adrenal extramedullary plasmacytoma.