Thiobacillus Denitrificans Research Articles

Effect of Signal Molecule Combined with Thiobacillus denitrificans on Simultaneous Removal of Nitrogen and Sulfur

The effects of Thiobacillus denitrificans combined with signal molecules on the removal of sulfide and nitrate was investigated. By adding signal molecules and T. denitrificans at the same, the total number of microorganisms increased, the removal of sulfide and nitrate was accelerated, and an increase in nitrogen gas and more stable accumulation of elemental sulfur was observed. The total number of microorganisms after the reaction was detected using fluorescence in situ hybridization (FISH) technique. In this experiment, the optimal concentration for the stable accumulation of elemental sulfur from six concentrations of signal molecules was revealed. Further, the effects of adding signal molecules, T. denitrificans, and their combination were analyzed at this concentration. The results showed that it was easier to accumulate elemental sulfur after the addition of 1.0 μmol·L-1 signal molecule. After adding both T. denitrificans and 1.0 μmol·L-1 signal molecules at a sulfide concentration of 200 mg·L-1, the removal of sulfide and nitrate increased to 99.8% and 96.9% at 72 h, respectively, and increases in nitrogen gas and sulfur were observed. The amounts of elemental sulfur and nitrogen gas reached to 59.0 mg and 80.0 mL, respectively, after adding 2.5 μmol·L-1 signal molecules at 72 h when the sulfide concentration was 300 mg·L-1. Under those conditions, the removal efficiency of sulfide and nitrate reached 99.0% and 93.9%, and the production of elemental sulfur and nitrogen reached 63.1 mg and 79.5 mL, respectively.

Simultaneous removal of sulfur and nitrogen compounds with methane production from concentrated latex wastewater in two bioreactor zones of micro‐oxygen hybrid reactor

AbstractBACKGROUNDA micro‐oxygen hybrid reactor (MOHR), a two‐zone reactor with suspended sludge zone (SZ) and attached biofilm zone (AZ) at its lower and upper parts respectively, was applied to simultaneously remove sulfur and nitrogen compounds with methane production from concentrated latex wastewater with high concentrations of sulfate (1200 mg L−1) and ammonium (235 mg L−1). Microbial community structures coupled with microbial activities were analyzed and discussed for clarifying relationships of microorganisms in both zones of the MOHR.RESULTSThe operating dissolved oxygen in the MOHR was controlled at 0.10–0.15 and 0.05 mg L−1 in the SZ and AZ respectively at various loading rates (LRs) and hydraulic retention times (HRTs). The average optimal overall performances of the MOHR for sulfate (SO42−), total Kjeldahl nitrogen (TKN) and chemical oxygen demand (COD) removal efficiencies were 90.3, 67.8 and 93.0% respectively at HRT 10 days. Elemental sulfur and nitrogen gas were evaluated as major end‐products at 90.2 and 67.9% respectively, with 0.27 L CH4 g−1 CODremoved of methane selectivity. The genera Desulfobulbus and Dongia as well as Thiobacillus denitrificans served as dominant microorganisms in biological sulfate removal. The largest abundance belonged to denitrifying microorganisms with nitrous oxide reductase (nosZ) as target gene.CONCLUSIONThe MOHR achieved high performance in simultaneous removal of sulfur and nitrogen compounds along with methane production at HRT 10 days. Microorganisms involved in sulfur and nitrogen compound removal processes were more prevalent in the SZ than in the AZ, whereas methanogenic activity was higher in the AZ than in the SZ. © 2019 Society of Chemical Industry

Characterisation of thiocyanate degradation in a mixed culture activated sludge process treating coke wastewater

Microbial degradation of thiocyanate (SCN−) has been reported to suffer from instability highlighting the need for improved understanding of underlying mechanisms and boundaries. Respirometry, batch tests and DNA sequencing analysis were used to improve understanding of a mixed culture treating coke wastewater rich in SCN−. An uncultured species of Thiobacillus was the most abundant species (26%) and displayed similar metabolic capabilities to Thiobacillus denitrificans and Thiobacillus thioparus. Thiocyanate was hydrolysed/oxidised to NH4+-N, HCO3− and SO42−. Nevertheless, at 360–2100 mg SCN−/L a breakdown in the degradation pathway was observed. Respirometry tests demonstrated that NH4+-N was inhibitory to SCN− degradation (IC50: 316 mg/L). Likewise, phenol (180 mg/L) and hydroxylamine (0.25–16 mg/L) reduced SCN− degradation by 41% and ca. 7%, respectively. The understanding of the SCN− degradation pathways can enable stable treatment efficiencies and compliance with effluent of <4 mg SCN/L, required by the Industrial Emissions Directive.

Light-driven nitrous oxide production via autotrophic denitrification by self-photosensitized Thiobacillus denitrificans

N2O (Nitrous oxide, a booster oxidant in rockets) has attracted increasing interest as a means of enhancing energy production, and it can be produced by nitrate (NO3−) reduction in NO3−-loading wastewater. However, conventional denitrification processes are often limited by the lack of bioavailable electron donors. In this study, we innovatively propose a self-photosensitized nonphototrophic Thiobacillus denitrificans (T. denitrificans-CdS) that is capable of NO3− reduction and N2O production driven by light. The system converted >72.1 ± 1.1% of the NO3−-N input to N2ON, and the ratio of N2O-N in gaseous products was >96.4 ± 0.4%. The relative transcript abundance of the genes encoding the denitrifying proteins in T. denitrificans-CdS after irradiation was significantly upregulated. The photoexcited electrons acted as the dominant electron sources for NO3− reduction by T. denitrificans-CdS. This study provides the first proof of concept for sustainable and low-cost autotrophic denitrification to generate N2O driven by light. The findings also have strong implications for sustainable environmental management because the sunlight-triggered denitrification reaction driven by nonphototrophic microorganisms may widely occur in nature, particularly in a semiconductive mineral-enriched aqueous environment.

Whole-cell cascade for the preparation of enantiopure β-O-4 aryl ether compounds with glutathione recycling

Bacterial β-etherases and glutathione lyases are glutathione-dependent enzymes that catalyze the selective cleavage of β-O-4 aryl ether bonds found in lignin. Their glutathione (GSH) dependence is regarded as major limitation for their application in the production of aromatics from lignin polymer and oligomers, as stoichiometric glutathione amounts are required. Thus, recycling of the GSH cofactor by a NAD(P)H-dependent glutathione reductase was proposed previously. Herein, the use of a whole-cell catalyst was studied for efficient β-O-4 aryl ether bond cleavage with intracellular GSH supply and recycling. After optimization of the whole-cell catalyst as well as reaction conditions, up to 5 mM lignin model substrate 2,6-methoxyphenoxy-α-veratrylglycerone (2,6-MP-VG) were efficiently converted into 2,6-methoxyphenol (2,6-MP) and veratryl glycerone (VG) without addition of external GSH. Unexpectedly, no glucose supply was required for glutathione recycling within the cells up to this substrate concentration. To demonstrate the applicability of this whole-cell approach, a whole-cell cascade combining a stereoselective β-etherase (either LigE from Sphingobium sp. SYK-6 or LigF-NA from Novosphingobium aromaticivorans) and a glutathione lyase (LigG-TD from Thiobacillus denitrificans) was employed in the kinetic resolution of racemic 2,6-MP-VG. This way, enantiopure (S)- and (R)-2,6-MP-VG were obtained on semi-preparative scale without the need for external GSH supply.

Biological oxidation of iron sulfides.

The biological oxidation of minerals and ores, called bioleaching, has been studied for the last decades to solubilize metals and recover them. In particular, iron sulfides are the most studied ores for an optimum extraction of different metals, such as copper or zinc. The use of chemolithotrophic bacteria, as Acidothiobacillus ferrooxidans, to oxidize both iron and sulfur species in aerobic conditions and at acidic pH shows promising results. In the field of heritage preservation, the development of "green" treatments is more and more studied. Waterlogged archeological wood presents an accumulation of iron sulfides within its structure, which, after exposition to oxygen, lead to salt precipitation and acidification and so to the degradation of the wooden artifact. A new extraction method, based on the dissolution of iron sulfides by the use of bacteria could be an alternative to the current chemical extraction methods, as being more respectful and ecological. While A. ferrooxidans is very effective in mines and groundwater, in the field of conservation-restoration of wood, Thiobacillus denitrificans is a better candidate as it grows at neutral pH, which is less aggressive for organic substrates (wood here). Preliminary studies show the efficiency of T. denitrificans for the dissolution of iron sulfides, as the concentration of nitrates used as electron donors decreases while the concentration of sulfates produced increases without degrading the wooden matrix. Long-term behavior should be studied to assess the stability of the artifacts after treatment.

Birnessite modified graphite cathode toward efficient autotrophic denitrification of Thiobacillus denitrificans in bioelectrochemical system

Birnessite modified graphite cathode toward efficient autotrophic denitrification of Thiobacillus denitrificans in bioelectrochemical system

Identification of Thiobacillus bacteria in agricultural soil in Iran using the 16S rRNA gene

Thiobacillus, as useful soil bacteria, plays an important role in sulfur cycling. The purpose of this study was to identify the species Thiobacillus thioparus, Thiobacillus novellas and Thiobacillus denitrificans in rainfed and irrigated lands soil in Ajabshir, Ilam, Qorveh, Rojintaak, Sonqor, Kermanshah and Research Farm of Razi University in Iran. Sampling was performed as randomized completely with three replications at depth of 0-30cm. The Thiobacillus species were determined via 16S rRNA characteristics. The results of agarose gel electrophoresis indicated that T. thioparus was the highest amount in the irrigated land in Research Farm and its lowest amount was in the Rojintaak rainfed land. These species not found in four locations and conditions including the Ajabshir irrigated, Qorveh rainfed, Research Farm rainfed and Rojintaak irrigated lands. The results of the T. novellas indicated that this was found in Ilam irrigated, Qorveh rainfed, Research Farm irrigated, Rojintaak irrigated and Rojintaak rainfed lands. The highest and lowest amount of T. novellas was indicated in the Rojintaak and Ilam irrigated lands respectively. The T. denitrificans gene showed that this bacterium was observed only in both samples of Ajabshir. Our study showed that Thiobacillus was not detected in all of the soils. If sulfur fertilizer is given to the soil without this bacterium, it is necessary to use sulfur fertilizer with Thiobacillus bacteria inoculation for better sulfur oxidation.

The Electrochemically Active Arsenic Oxidising Bacterium Ancylobacter sp. TS‐1

AbstractThe study of electrochemically active microorganisms (EAMs) has revealed the metabolic versatility of chemolithoautotrophic microorganisms in bioelectrochemical systems (BESs). However, direct electron uptake from electrodes to support bacterial growth has been suggested only for the iron oxidiser Mariprofundus ferrooxydans, the sulfur oxidiser bacterium Thiobacillus denitrificans and the iron/sulfur oxidiser Acidithiobacillus ferrooxidans. To the best of our knowledge, chemolithoautotrophic arsenite oxidisers (CAOs) have not been reported as biocathodic EAM. This paper studies the electrochemical activity of Ancylobacter sp. TS‐1, a new CAO. The electrochemical capacity of TS‐1 was evidenced by linear sweep voltammetry tests, revealing a peak of cathodic current dependent on biomass concentration at −500 mV (vs. Ag/AgCl, at pH 7.2); and by chronoamperometry, showing an increase in the cathodic current over time. Cathodic currents obtained for TS‐1 (9.5±2.4 μA/cm2) are comparable with values previously reported for A. ferrooxidans (∼27 μA/cm2) and M. ferrooxydans (∼8 μA/cm2). Scanning electron micrographs show TS‐1 cells on electrodes during the chronoamperometry, suggesting biofilm development of strain TS‐1 when using the cathode only as an electron donor. Hence, TS‐1 not only enlarges the list of known biocathodic EAMs, but also paves the way for novel BES applications on arsenic electro‐bioaugmentation and bioremediation.

Biological oxidation of sulfur compounds in artificially degraded wood

Marine and lacustrine archaeological waterlogged wood encounters serious problems after excavation due to the accumulation of sulfur and iron compounds during burial. Exposure of these compounds to oxygen results in precipitation of salts and acidification, which can lead to serious structural damage, and ultimately the loss of important cultural heritage. In this study, we evaluated the capacity of the bacterium Thiobacillus denitrificans to transform sulfur compounds commonly found in waterlogged wooden objects, to more readily extractable compounds thereby eliminating the threat of degradation. Oak samples, impregnated with a solution containing iron(II) and sulfides, were used to assess the efficiency of the bacterial treatment. The model wood samples were characterized before and after treatment using different techniques such as ESEM-EDS, micro-Raman spectroscopy, XRD and Sy-XRF mapping. Before treatment, mackinawite (FeS) and mineral sulfur (α-S8) were detected in the impregnated wood. After treatment with T. denitrificans, even though some mineral sulfur remained in the samples, the predominant phase corresponded to oxidized sulfur. This demonstrates that T. denitrificans was able to use the reduced sulfur compounds present in the wood samples as an energy source, thereby producing more soluble oxidized sulfur compounds. In addition, non-invasive techniques such as Fourier transform infrared (FTIR) spectroscopy, were carried out to assess the consequences of the biological treatment on the wood structure. No negative effect on the wood was detected after the treatment in comparison with the reference-impregnated wood. This study demonstrates the feasibility of a biotechnological procedure for the preventive extraction of sulfur species from archaeological waterlogged wood.

Multivariate and Multiscale Approaches for Interpreting the Mechanisms of Nitrous Oxide Emission during Pig Manure-Wheat Straw Aerobic Composting.

Nitrous oxide (N2O) emission during composting causes nitrogen loss and air pollution. The interpretation of N2O emission mechanisms will help to customize composting strategies that mitigate climate change. At pile and particle scales, this study characterized N2O emission-related variables (gases, ions, and microbes) and their correlations during pig manure-wheat straw aerobic composting. Pile-scale results showed that N2O emission mainly occurred in mesophilic, thermophilic, and cooling phases; the nitrification by ammonia-oxidizing bacteria ( AOB) and nitrite-oxidizing bacteria ( NOB) coexisted with the denitrification by denitrificans ( DEN); the major NOB and DEN were Nitrobacter ( NOB_Nba) and Thiobacillus denitrificans ( DEN_Tb), respectively. The mechanisms of nitrification, nitrifier denitrification, and anaerobic denitrification in composting particles were initially visualized by confocal laser scanning microscopy: Betaproteobacteria ( AOB_ Beta) sporadically distributed on the outer area of the particles, NOB_Nba internally attached to AOB_ Beta, and Nitrosomonas europea/ Nitrosomonas eutropha ( AOB_eu) and DEN_Tb concentrated in the interior. Correlation analysis of the variables showed that the distribution area of AOB_eu was proportional to N2O emission ( R2 = 0.84); AOB not only participated in nitrification but also nitrifier denitrification, and N2O formation was mainly from nitrifier denitrification by AOB_eu during the mesophilic-thermophilic phase and from denitrification by AOB_eu and DEN during the cooling phase.

DEVELOPMENT OF AUTOTROPHIC DENITRIFICATION FOR NITRATE-CONTAMINATED INDUSTRIAL WASTEWATER

An autotrophic denitrification system was developed for treatment wastewater from steel industry. The aim was to evaluate the kinetics and capability of the system in reducing nitrate content in such industrial wastewater. The experiments were conducted in batch suspension and continuous runs. The denitrification kinetics in suspension runs obeys first order reaction with the rate constant k1 and k2 were determined to be 0.014 and 0.004 g-N/m3.d, respectively. The continuous runs used a column packed with mixture of granular sulfur and limestone. The microbes of Thiobacillus denitrificans were attached on the surface of granular sulfur in the form of biofilm. The biofilm thickness was investigated to be approximately 40 μm. The denitrification kinetics in the packed column obeys half-order reaction with the rate constant k of 0.172 g-N1/2.m1/2/(kg-S.d). The lower denitrification extent of industrial wastewater compared with the synthetic wastewater might be due to the lack of alkalinity. Keywords: Autotrophic denitrification, sulfur, packed column, thiobacillus denitrificans.

Structural characterization of the bacterial proteasome homolog BPH reveals a tetradecameric double-ring complex with unique inner cavity properties

Eukaryotic and archaeal proteasomes are paradigms for self-compartmentalizing proteases. To a large extent, their function requires interplay with hexameric ATPases associated with diverse cellular activities (AAA+) that act as substrate unfoldases. Bacteria have various types of self-compartmentalizing proteases; in addition to the proteasome itself, these include the proteasome homolog HslV, which functions together with the AAA+ HslU; the ClpP protease with its partner AAA+ ClpX; and Anbu, a recently characterized ancestral proteasome variant. Previous bioinformatic analysis has revealed a novel bacterial member of the proteasome family Betaproteobacteria proteasome homolog (BPH). Using cluster analysis, we here affirmed that BPH evolutionarily descends from HslV. Crystal structures of the Thiobacillus denitrificans and Cupriavidus metallidurans BPHs disclosed a homo-oligomeric double-ring architecture in which the active sites face the interior of the cylinder. Using small-angle X-ray scattering (SAXS) and electron microscopy averaging, we found that BPH forms tetradecamers in solution, unlike the dodecamers seen in HslV. Although the highly acidic inner surface of BPH was in striking contrast to the cavity characteristics of the proteasome and HslV, a classical proteasomal reaction mechanism could be inferred from the covalent binding of the proteasome-specific inhibitor epoxomicin to BPH. A ligand-bound structure implied that the elongated BPH inner pore loop may be involved in substrate recognition. The apparent lack of a partner unfoldase and other unique features, such as Ser replacing Thr as the catalytic residue in certain BPH subfamilies, suggest a proteolytic function for BPH distinct from those of known bacterial self-compartmentalizing proteases.

Influence of pH, EDTA/Fe(II) ratio, and microbial culture on Fe(II)-mediated autotrophic denitrification.

Fe(II)-mediated autotrophic denitrification with four different microbial cultures under different pH and EDTA/Fe(II) conditions was investigated in batch bioassays. Initially, the highest nitrate removal (72%) was achieved with an activated sludge inoculum. The use of pure cultures of Pseudogulbenkiania strain 2002 and Thiobacillus denitrificans resulted in a 55 and 52% nitrate removal, respectively. No denitrification was observed for a mixed culture dominated by Thiobacillus thioparus and T. denitrificans. A longer enrichment on Fe(II) and the supplementation of thiosulfate as additional electron donor were needed to stimulate the denitrifying activity of the Thiobacillus-mixed culture. A second subculture on Fe(II) as sole electron donor resulted in higher denitrification efficiencies for all microbial cultures. In particular, nitrate removal reached up to 84% with a specific nitrate removal rate of 1.160mM·(g VSS·day)-1 in the bioassays seeded with the Thiobacillus-mixed culture. All cultures were favored by decreasing the EDTA/Fe(II) molar ratio from 2.0 to 0.5. The most significant denitrification enhancement was observed for the Pseudogulbenkiania species, indicating a lower tolerance to EDTA. The two pure cultures effectively maintained denitrification at pH7.0 and were more sensitive to a pH decrease. Conversely, the optimal pH was 6.0 for the Thiobacillus-mixed and activated sludge cultures.

Influence of Fe3+ Ions on Nitrate Removal by Autotrophic Denitrification Using Thiobacillus denitrificans

Influence of Fe3+ Ions on Nitrate Removal by Autotrophic Denitrification Using Thiobacillus denitrificans

Coupling autotrophic denitrification with partial nitritation-anammox (PNA) for efficient total inorganic nitrogen removal

The performance of and the microbial ecology in an integrated lab scale set up comprising of a PN/A bioreactor and an elemental sulfur-supported packed bed autotrophic denitrification (ESSAD) are reported. The PN/A reactor exhibited an average removal rate of 0.56±0.103kgNm−3d−1, whereas the ESSAD reactor removed an average of 0.0018kg NO3−-Nm−3d−1. The combined average removal rate was 0.6kgNm−3d−1, yielding an overall total inorganic nitrogen efficiency of 97%. Based on 16S rRNA gene clone libraries from the ESSAD reactor, the extracted Operational Taxonomic Units (OTUs) formed a clade with Thiobacillus denitrificans sp. indicating a common ancestral relationship. High throughput amplicon sequencing targeting V3 region of 16S rRNA gene for the biofilm in the ESSAD also revealed an abundance of the Thiobacillus genus. Additionally, 16s rRNA amplicon sequencing of the genomic DNA from the PN/A reactor reflected a dominance of the Planctomycetes phylum.

Implementation of sulphur denitrification in a large-scale fully recirculated cold-SW aquarium: A sustainability practice

Autotrophic sulphur-based denitrification (ASD) was evaluated to control nitrate accumulation in the marine aquatic exhibit of the Biodome de Montreal (Montreal, QC, Canada). Different substrates (two sulphur and two calcareous sources were tested), flow-rates and configurations (homogenous and low to high stratification levels) were evaluated that successively improved nitrate removal rates (g N-NO3- · day-1) and/or start-up time. Despite pronounced suboptimal conditions for anaerobic denitrifying bacteria, Thiobacillus denitrificans i.e. saline (24 PSU), cold (5-10oC) and aerated waters, our R&D efforts lead to the development of an efficient, simple, custom-made ASD unit adapted to a priori unfavourable conditions. The Rocky Shore exhibit (25 m3) housing the most sensitive marine aquatic species of our live collections (mostly invertebrates) previously operated at ? 50 mg N-NO3- · L-1 is now at ~ 20 mg N-NO3- · L-1. Considering the significant economic and environmental gains achieved (e.g. sustainability) following the implementation of this technology at a small-scale on the Rocky Shore exhibit (100 kg of sulphur), a 10-fold upscaling (1000 kg of sulphur) ASD unit to be connected to the La Baie exhibit (2500 m3) is planned.

Effects of different nickel species on autotrophic denitrification driven by thiosulfate in batch tests and a fluidized-bed reactor

Nickel is a common heavy metal and often occurs with nitrate (NO3−) in effluents from mining and metal-finishing industry. The present study investigates the effects of increasing concentrations (5–200mgNi/L) of NiEDTA2− and NiCl2 on autotrophic denitrification with thiosulfate (S2O32−) in batch tests and a fluidized-bed reactor (FBR). In batch bioassays, 50 and 100mgNi/L of NiEDTA2− only increased the transient accumulation of NO2−, whereas 25–100mgNi/L of NiCl2 inhibited denitrification by 9–19%. NO3− and NO2− were completely removed in the FBR at feed NiEDTA2− and NiCl2 concentrations as high as 100 and 200mgNi/L, respectively. PCR-DGGE revealed the dominance of Thiobacillus denitrificans and the presence of the sulfate-reducing bacterium Desulfovibrio putealis in the FBR microbial community at all feed nickel concentrations investigated. Nickel mass balance, thermodynamic modeling and solid phase characterization indicated that nickel sulfide, phosphate and oxide precipitated in the FBR during NiCl2 injection.

Impact of operating condition on the denitrifying bacterial community structure in a 3DBER-SAD reactor.

Two main operating parameters (influent C/N ratio and electric current intensity) were examined for their impacts on the denitrifying bacterial community structure in an integrated system of three-dimensional biofilm-electrode reactor and sulfur autotrophic denitrification (3DBER-SAD). It was found that genus β-proteobacteria played a leading role under different operating conditions. The influent C/N ratio illustrated a great impact on denitrifying bacteria diversity. When the C/N ratio decreased from 1.07 to 0.36, the Shannon-Wiener index and Simpson index increased from 2.44 to 2.71 and from 0.89 to 0.92, respectively, while the proportion of heterotrophic denitrifying bacteria Thauera decreased from 61.4 to 21.1%, and the sulfur autotrophic denitrifying bacteria (e.g., genus Sulfuricella and Thiobacillus denitrificans) increased from 3.5 to 19.3%. In terms of the impact of electric current intensity, the Shannon-Wiener index and Simpson index decreased from 2.71 to 2.63 and from 0.92 to 0.90, respectively, as the current intensity increased from 60 to 400mA.

Identify biosorption effects of Thiobacillus towards perfluorooctanoic acid (PFOA): Pilot study from field to laboratory

The concentration of Perfluoroalkyl acids (PFAAs) and the bacterial community composition along the Xiaoqing River were explored with HPLC-MS/MS and Illumina high-throughput sequencing in present study. The results showed that perfluorooctanoic acid (PFOA) was the predominant PFAAs in all sediment samples, and high level of PFOA could lead to an evident increase in the abundance of Thiobacillus. Thiobacillus was identified with the survival ability in high concentrations of PFOA accordingly. Therefore, Thiobacillus thioparus and Thiobacillus denitrificans were selected as receptors to design indoor biosorption experiment. The growth curves under different PFOA concentrations and residual rates of PFOA in the processes of cultivation were analyzed. The results showed that upwards concentrations of PFOA below 5000 ng/L led to an obvious increase in the growth rate of T. thioparus. Whereas PFOA promoted the growth of T. denitrificans in a relatively limited range of concentration, and the effect was not obvious. The addition of different concentrations of PFOA had no apparent effects on pH values in the media of both T. thioparus and T. denitrificans. The concentrations of PFOA in liquid media reduced after the process of bacteria culturing. The removal rates of T. thioparus and T. denitrificans to PFOA were 21.1–26.8% and 13.5–18.4%, respectively. The current findings indicated that T. thioparus could play a significant role as potential biosorbent with the ability to eliminate PFOA effectively in aquatic environment, which would provide novel information for PFOA ecological decontamination and remediation.