Thiabendazole Treatment Research Articles

Strongyloidiasis in North Queensland: re‐emergence of a forgotten risk group?

To describe the clinical presentation, diagnosis and management of strongyloidiasis in northern Queensland, and to identify predisposing factors for this infection. A five-year retrospective study of medical records of all cases diagnosed in a regional hospital microbiology laboratory, with follow-up by interview where possible. The Townsville General Hospital. A total of 14 cases of strongyloidiasis were identified, with patients ranging in age from 7 months to 59 years. Infected patients included nine Aborigines and two Vietnamese refugees, both high-risk groups. Of three Caucasians diagnosed, one was a war veteran, but the other two did not have significant travel histories; all three had lived or worked under insanitary conditions. In only one case was strongyloidiasis suspected; in most, it was diagnosed because of diarrhoea or blood eosinophilia which could not be related to the presenting illness. Thiabendazole treatment was effective in most cases. In northern Australia, strongyloidiasis can be acquired locally by Caucasians who live in unhygienic circumstances. It should be suspected in any person with unexplained abdominal pain, diarrhoea, cutaneous symptoms or blood eosinophilia, and the laboratory must be informed of the provisional diagnosis.

Reduced effect of chemotherapy of strongyloidiasis in patients with concurrent HTLV-I infection in Okinawa, Japan.

The effect of concurrent HTLV-I infection on the efficacy of anti-Strongyloides chemotherapy has been tested in a prognostic study on 96 patients with uncomplicated strongyloidiasis. The efficacy of treatment with pyrvinium pamoate and thiabendazole was found to be significantly low in the HTLV-I seropositive patients, as compared to that in the seronegative group. The cure rates in the HTLV-I seropositive patients, when assessed by both faecal examination and serological testing, were only 8.6% for pyrvinium pamoate treatment and 35.0% for thiabendazole treatment. On the other hand, the cure rates were 31.3% for pyrvinium pamoate treatment and 57.1% for thiabendazole treatment in the seronegative group.Although antibody response against Strongyloides did not decrease among the HTLVI seropositive patients, the total serum IgE levels, as well as eosinophil counts in peripheral blood, were relatively low in the seropositive group. Abnormal lymphocytes were demonstrated in 38.5% of the patients with concurrent HTLV-I infection. The presence of abnormal lymphocytes, however, appeared not to correlate with the therapeutic efficacy. A possible connection between the poor efficacy and depressed immune responses provided by the concurrent HTLV-I infection is discussed. The low efficacy in the HTLV-I seropositive patients also provides a causal explanation for the significant accumulation of patients with concurrent HTLV-I infection in Okinawa.

Effect of Haemonchus contortus infection on the clearance of antipyrine, sulfobromophthalein, chloramphenicol, and sulfathiazole in lambs

SUMMARY A study was made to determine the effect of Haemonchus contortus parasitic infection in lambs on the clearance of several IV administered drugs. Clearance of sulfobromophthalein or sulfathiazole from the plasma of lambs was unaffected by infection with H contortus. Clearance of antipyrine was enhanced by the infection, and thiabendazole treatment did not alter this effect. Clearance of chloramphenicol (cap), administered as the succinate ester (caps), was not changed by the infection, but it was increased after treatment with thiabendazole. Changes in the mean body residence time and initial plasma concentration of caps and cap after treatment with thiabendazole indicate that hydrolysis of caps to cap was reduced. High concentrations of caps apparently enhanced its own elimination directly rather than via the expected sequence involving hydrolysis, glucuronidation, and excretion of cap-glucuronide. Enhanced clearance of antipyrine following infection of lambs with H contortus can be explained in at least 2 ways. First, it is possible that the lambs did not have mature amounts of hepatic drug metabolizing enzyme activity as reported by other investigators, which may be explained by breed differences or animal husbandry practices. Second, infection of lambs by H contortus may have triggered an inductive response in hepatic cytochrome P- 450-mediated activities, which might result via a generalized enhancement in hepatic protein synthesis associated with the physiologic response to replace plasma proteins and other blood components lost through gastrointestinal hemorrhage caused by the active feeding of adult worms. Other phase-II reactions such as acetylation, glucuronidation, and glutathione-S-transferase apparently were not affected.

Selection for thiabendazole resistance in Ostertagia spp. By low efficiency anthelmintic treatment

Thiabendazole (TBZ) treatment of grazing weaners infected with a predominantly susceptible strain of Ostertagia reduced worm burdens by 90%. The surviving sub population had a level of resistance four times greater than the original population when compared to similarly infected, but untreated, weaners. TBZ treated and control weaners were relocated to worm-free pasture so that the contamination on the plots grazed by TBZ treated weaners was from the survivors of the drench. The anthelmintic treated groups were subsequently managed under a two summer drench programme while control groups remained so throughout the experiment. Monitoring both parasite population size and the degree of resistance over the following 2 years indicated that after three anthelmintic treatments, resistance developed to a level where TBZ was ineffective. When compared to control groups, a mean reduction in population size of around 80% was maintained in the TBZ treated groups for 1 year. This difference, however, disappeared by the second year. A similar study over 1 year with a moderately TBZ resistant strain of Ostertagia indicated that TBZ treatment produced no significant reduction in population size but more than doubled the level of resistance. The study indicated the critical need for highly efficient anthelmintic treatment, particularly in situations where, through ecology or management, post treatment contamination is likely to contribute substantially to future generations of worms.

Strongyloidiasis in an Institution for Mentally Retarded Adults

A serologic and epidemiologic survey was undertaken to explain the high incidence of eosinophilia (14.1%) in nine of 64 residents of a facility for mentally retarded adults in Pennsylvania. Seven residents had antibodies to Strongyloides stercoralis as determined by an IgG enzyme-linked immunosorbent assay system (10.9%) and five (71.4%) of this subset had persistent eosinophilia, suggesting a strong association. Sex, race, age, ward, degree of retardation, and medication were not associated with eosinophilia. Stool examination revealed S stercoralis larvae in two residents with S stercoralis antibodies and eosinophilia. Follow-up studies after thiabendazole treatment of the residents with S stercoralis antibodies showed resolution of eosinophilia and disappearance of S stercoralis antibodies in most. Eosinophilia in an adult institutional setting may be associated with strongyloidiasis, even in a nonendemic area. The enzyme-linked immunosorbent assay for antibody to S stercoralis may be useful in diagnosing or monitoring therapy of strongyloidiasis.

Responses to Controlling Gastrointestinal Parasitism in Dairy Cattle

Sixty-eight Holstein heifers, paired on milk production potential and season of birth, were randomly assigned within pairs to a control or treatment group to evaluate the effect of raising replacement heifers on a prophylactic anthelmintic regimen. The anthelmintic, thiabendazole (6.6g/100kg body weight), was administered orally as a paste at 2.5, 3.5, 6, 9, and 14 mo of age and at parturition. The control group received no thiabendazole. Fecal samples were collected from both groups at the time of thiabendazole treatment. Days to first service were 457.1 and 457.8 for the control and treatment groups. There were no differences in services per conception or days to first calving. Nematode eggs per 5g of feces were similar at 2.5 mo (.6 and .8), 3.5 mo (38.6 and 22.4), 6 mo (40.2 and 43.1), 9 mo (39.0 and 22.4), 14 mo (55.2 and 22.4), or parturition (16.5 and .4). There were no differences in body weight changes over the first 18 mo of age. Subsequent first lactations were similar (9422 and 8710kg 305-d mature equivalent, for twice daily milking) as was actual milk production for the first 120 d of lactation. Under these environmental conditions and apparently low parasitism in this herd, thiabendazole did not substantially influence nematode egg count and had no effects on body weight gains or milk production.

Relationship of gastrointestinal histology and mucus antiparasite activity with the development of resistance to trichostrongyle infections in sheep

Changes in the numbers of globule leucocytes, mast cells, eosinophils and goblet cells in the gastrointestinal mucosa were examined in relation to the development of resistance and elimination of nematodes in grazing sheep in their firsty year of life. Sheep immunised against Trichostrongylus colubriformis, and sheep treated with dexamethansone were also examined. A strong association between resistance to infection and the presence of globule leucocytes was found. In contrast, the numbers of mast cells or goblet cells were not correlated with resistance. Globule leucocyte and eosinophil numbers were also correlated with antiparasite activity in mucus. Immunising infections of T. colubriformis given to 10-month-old sheep, their duration limited by thiabendazole treatment, gave rise to considerable immunity to homologous challenge infections. Larvae that developed to the 4th stage were as effective at stimulating immunity as those that developed to the 5th stage. Dexamethasone treatment abrogated resistance to trickle challenge infection with T. colubriformis and reduced mucosal globule leucocyte and mast cell numbers. After cessation of drug treatment, the re-establishment of resistance and adult worm elimination were associated with repopulation of the mucosa with large numbers of globule leucocytes and high antiparasite activities in mucus.

The effect of thiabendazole seedpiece treatment on Monona potatoes in Wisconsin

Seedpiece protectants containing thiabendazole (TBZ) were deleterious to stand establishment and yield and were associated with the formation of secondary tubers from seedpieces on Monona potatoes in four of five years (1980–1984) in Wisconsin. Seed tubers treated with a pre- and/or poststorage mist application of TBZ prior to a post-cutting treatment with a dust formulation containing 0.5% TBZ were most severely affected. The negative effects of pre- and post-cutting TBZ treatment on yield were additive. Observations in grower fields during 1981 indicated that cultivars differed in their sensitivity to this fungicide. Ontario, LaChipper, and Monona were most severely affected while problems were not observed on the Superior and Russet Burbank cultivars.

Metabolite concentrations in plasma following treatment of cattle with five anthelmintics

Plasma concentrations of anthelmintics and their metabolites were determined after cattle were treated at recommended dose rates and routes of administration. Fenbendazole, oxfendazole, febantel, albendazole and thiabendazole were given orally and oxfendazole was also administered with an intraruminal injector. After fenbendazole, oxfendazole and febantel were administered, fenbendazole, oxfendazole and fenbendazole sulphone were all detected in plasma in each case. However, there were marked differences between the three anthelmintics in the peak concentrations and areas under the plasma concentration/time curve (AUC) of these three metabolites. Intraruminal administration of oxfendazole produced higher AUC for fenbendazole and fenbendazole sulphone than did oral administration. Albendazole sulphoxide and sulphone were detected in cattle plasma after albendazole administration but no parent drug was present. These metabolites disappeared more rapidly in cattle than has been reported for sheep. Only 5(6)hydroxythiabendazole was detected in cattle plasma after thiabendazole treatment.

Wound Response of Ulmus americana I: Results of Chemical Injection in Attempts to Control Dutch Elm Disease

AbstractConcern for injury to elms from chemical injection led to an evaluation of the impact of wounding and exposure to liquid fungicide on the wound response ofUlmus americana. Small elms were injected with thiabendazole (TBZ) plus solvent, its solvent alone, or water. The external symptoms of injury following injection were observed over a 23 month period and the internal response to injury was recorded over a 33 month period from dissection of stems during the growing and dormant seasons. Wounds with water injection had small columns of discolored wood while wounds with chemical injections had large columns of discolored wood. TBZ treatment was toxic to woody tissues and caused the largest amount of discolored tissue in trees injected in May and June. The extensive amount of discolored wood associated with injected fungicide indicated that precautions must be taken such that injections will not limit future distribution of fungicide, decrease storage and transport capacities and predispose trees to other infectious agents.

Reducing Susceptibility of Grapefruit to Chilling Injury during Cold Treatment

Abstract With the expected ban on ethylene dibromide fumigation, cold exposure remains the only quarantine treatment for citrus fruit against the Mediterranean fruit fly. Following a cold treatment, ‘Marsh’ grapefruit (Citrus paradisi Macf.) developed chilling injury (Cl), mostly in the form of slight peel pitting on 3% to 10% of the fruit depending on the season and on other factors. There was no difference in the incidence of Cl between fruit treated at 0°C for 10 days or at 2.2° for 16 days as regulations require. The cold treatment also enhanced decay development during long-term storage of the fruit at 11°. Mold rots developed on Cl peel pitting, and their incidence increased from 1.7% to 3.5% during a storage period of 12 weeks. The presence of the fungicide Thiabendazole (TBZ) in the wax coating of the fruit reduced the incidence of Cl by more than 50%. Delayed cooling, i.e., keeping the freshly harvested packed fruit for 6 days at 17°C prior to initiation of cold treatment, reduced the incidence of Cl by the same extent. By combining a TBZ treatment with delayed cooling, the susceptibility of grapefruit to Cl can be reduced, and cold treatment can be practiced with a low risk of Cl and subsequent decay development.

Effect of thiabendazole treatment on feed intake, digestibility and selected blood values in lactating dairy cows.

Observations of feed intake, digestibility, production and selected blood values were made during the winter housing period on 10 lactating dairy cows, naturally infected with gastrointestinal roundworms, which were either left untreated or dosed on two occasions with thiabendazole in-feed. While treatment had no significant effect on appetite or production, there was a 9.8 per cent improvement in cellulose digestibility (P less than 0.05). An increase in circulating cholesterol in treated cows was associated with the improved utilisation of cellulose. It is considered unlikely that thiabendazole's primary anthelmintic activity was responsible for this effect. Treated cattle also exhibited a significant improvement in thyroid function during the course of the study compared with control animals. This might account for the increase in milk production reported in several trials following anthelmintic treatment of dairy cows.

Field efficacy of a morantel sustained release bolus for control of gastrointestinal nematodes in yearling steers

The efficacy of a morantel sustained release bolus (MSRB) for control of gastrointestinal nematodes in yearling steers was evaluated over a 6-month grazing period commencing on 26 March 1982. Three groups of 15 steers were allotted to the following treatments: Group 1 — one MSRB at start of trial; Group 2 — one therapeutic dose of thiabendazole at start of trial; Group 3 — untreated control. The treatment groups were grazed separately. Parasite egg counts (EPG), herbage larval counts, pepsinogen levels and weight gains were monitored. Every other month, sets of 2 parasite-free tracer calves were placed in the pasture grazed by each treatment group and allowed to graze for 3 weeks before being subsequently necropsied for worm counts. At the end of the trial, 6 animals from each group were also necropsied for worm counts. The MSRB treatment resulted in significantly lower egg counts, fewer infective larvae on pasture, lower pepsinogen levels and lower worm burdens in tracer calves than was the case for the untreated group, but generally the levels were not significantly different from those associated with the thiabendazole treatment. The mean weight gain for the MSRB treated steers showed a significant advantage (70.9 lb) over the untreated animals, but was not significantly different from those which received thiabendazole. Total worm counts at the end of the trial were not different from any treatment group.

The Effect of the Intestinal Phase of Trichinella spiralis on the Open-Field Behavior of Mice

The study assessed the impact of the adult phase of Trichinella spiralis on the open-field behavior of mice. Thiabendazole treatment was used to chemosterilize adult worms in vivo. Untreated mice exhibited a marked decline in their ambulatory and exploratory activities (from day 14 post-infection until the end of the experiment). In contrast, infected mice treated with thiabendazole did not exhibit any behavioral alterations. This suggested that the adult phase of T. spiralis did not contribute to behavioral pathology in the mouse host.

Título en español

Pedazos de 115 g de ñame Habanero (Dioscorea rotundata Poir) de alta calidad se trataron par una hora con fungicidas, 48 horas antes de sembrarlos para determinar el efecto sobre el brote y el rendimiento. Se escogió un predio experimental que no había estado sembrado de ñames previamente y estaba libre de nematodos parásitos del ñame. Se registró, 91 , 95 y 96% de brote en los pedazos tratados con captan + benomyl, captan, y captan + thiabendazole, respectivamente, comparado con 52% en los que no se trataron con fungicida (testigo). El brote temprano (5 semanas después de la siembra) fue mayor en los pedazos tratados con captan + thiabendazole (24%) que en los tratados con captan + benomyl (1%), con captan (2%) o el testigo (1%). A las 9 semanas, los tratamientos con fungicidas no fueron diferentes significativamente en el porcentaje de pedazos que brotaron (91-96%). Se determinaron correlaciones positivas entre el porcentaje de pedazos que brotaron y el rendimiento (r = 0.77**); el número total de tubérculos cosechados (r = 0.84**) y el peso medio de los tubérculos (r = 0.74**). El testigo produjo más tubérculos por planta (1.64) que los tratados con fungicidas (1.16-1.20). Las pérdidas en el rendimiento del testigo (48%) estuvieron asociadas con las pérdidas en el peso por tubérculo (37%) y con el total producido por área (20%). Se registró un mayor rendimiento tanto por planta como por área y por tubérculo en los que se trataron con captan + thiabendazole que con captan o captan + benomyl. Los factores que determinan la producción total y el tamaño de los tubérculos son el brote más temprano y el brote de un número mayor de pedazos. En este estudio aún cuando se seleccionaron tubérculos de alta calidad y se sembraron en condiciones óptimas, el brote y el rendimiento se redujo casi a la mitad cuando los pedazos no se trataron con un fungicida antes de sembrarlos.

The association between frequency of thiabendazole treatment and the development of resistance in field isolates of Ostertagia spp. of sheep

Martin P. J., Anderson N., Lwin T., Nelson G. and Morgan T. E. 1984. The association between frequency of thiabendazole treatment and the development of resistance in field isolates of Ostertagia spp. of sheep. International Journal for Parasitology 14: 177–181. Isolates of Ostertagia spp. were obtained from grazing sheep 3,4 and 5 years after nil, planned (five per year) and regular (3-weekly) treatments with thiabendazole (TBZ). Levels of resistance to TBZ were measured by an in vitro egg hatch assay and a controlled anthelmintic efficiency assay. Isolates from planned treatment groups showed an increase in the level of resistance; the lethal concentrations of TBZ to 50% of eggs (LC 50s) were 3, 3 and 6 times the LC 50s of isolates from nil treatment groups for years 3, 4 and 5 respectively. The LC 50s of isolates from regular treatment groups were 14 times higher than those from nil treatment groups in each year. To assess the potential for an increase in level of resistance, additional egg assays were done 14 days after treatment with 44 mg kg t̄1 of TBZ on sheep infected with the planned group isolates for each year. This treatment raised the LC 50S for years 3, 4 and 5 respectively by 3, 2 and 1–5 times the LC 50s of the same isolates which had not been exposed to additional TBZ treatment. The controlled anthelmintic efficiency assay using 44 mg kg t̄1 of TBZ produced a significant reduction in the number of adult and immature worms from the nil isolate but failed significantly to reduce the number of worms from the planned and regular isolates. A three component analysis resolved the nonlinear trends of the log dose-probit plots in egg hatch assays for isolates from planned treatment groups into subpopulations of susceptible, hybrid and resistant individuals each with different levels of resistance. The proportions of these subpopulations changed in accordance with the level of resistance observed in each year.

The elimination of equine strongyles and hematological and pathological consequences following larvicidal doses of thiabendazole

Twelve horses wer divided into three groups and given various doses of a mixed species strongyle inoculum, representing light, moderate, and heavy infctions. Three weeks after the larval inoculations, three animals from each group were given larvicidal doses of thiabendazole (TBZ) (440 mg kg −1 on two consecutive days); one animal from each group served as a non-medicated control. Treatment was repeated three weeks later. One treated animal from each group was designated for long-term study; others were necropsied to study adult and larval parasite loads. Six of the twelve animals with strongylosis developed moderate eosinophilia. TBZ given at 440 mg kg − on two consecutive days caused depression, lethargy, and anorexia which lasted for five days. Eosinopenia, lymphopenia, and neutrophilia occured in treated animals, and lasted for three days. During the course of TBZ treatment, one horse died from what appeared to be a mis-dosing or an anaphylactic reaction. At necropsy, active thrombi of the anterior mesenteric artery were seen in parasitized animals, but not in those treated with TBZ. Five out of seven medicated horses were completely free of adult and larval strongyle parasites. One had a few STrongylus edentatus larvae and another had small strongyles. No Strongylus vulgaris larvae or adults were recovered from any horse treated with TBZ.

Sensitivity of isolates of Trichinella to the drug thiabendazole

The sensitivity of three isolates of Trichinella spiralis (from pig, polar bear, and wolverine) and T. spiralis var. pseudospiralis to the anthelmintic thiabendazole (TBZ) was studied in male CD-1 mice. In vitro larval release by female worms isolated on day 7 following drug treatment (2–7 days) with various drug dosages (0.01–0.06%) showed that TBZ treatment resulted in sterility or a decrease in fecundity of the worms. Females of the pig isolate and T. spiralis var. pseudospiralis were chemosterilized with 0.05% TBZ and at 0.03% so were the two Arctic isolates (polar bear and wolverine), but chemosterilization was not permanent. Drug treatment during the disseminating phase (4–20 days) with low dosages (0.03–0.1%) was highly effective in reducing the number of muscle larvae at 40 days postinfection, a reduction that was similar for all Trichinella. Drug treatments (0.03–0.5%) during the early muscle phase (20–40 days) were ineffective with low dosages (<0.15%), but with a higher dosage (0.5%) 99 and 100% efficacy were noted for the two Arctic isolates and T. spiralis var. pseudospiralis, respectively. Larvae recovered at high dosages (>0.15%), were not infective to mice. Treatments with dosages of 0.03–0.1% from 40 to 60 days postinfection were only effective against the Arctic isolates. Of all the Trichinella studied, the pig isolate was the least sensitive to parenteral treatment.

Persistent Strongyloidiasis in an Immunodeficient Patient *

A 56-year-old woman with acquired, common variable immunodeficiency was found to have persistent gastrointestinal as well as pulmonary infection with Strongyloides stercoralis. Repeated courses of treatment with thiabendazole led to marked reduction or loss of Strongyloides stercoralis larvae, but cessation of treatment always led to recurrence of Strongyloides infection. Several small bowel biopsies showed normal villous architecture and little inflammatory response to presence of larvae. Interestingly, no definite symptomatology could be attributed to the Strongyloides infection. It was postulated that the lack of signs and symptoms of strongyloidiasis, as well as poor response to treatment, was related to the immunodeficiency state. With low-dose, long-term interrupted courses of thiabendazole treatment, the Strongyloides infection finally seemed to be cured.

Effects of a preventive and suppressive control scheme on the development of thiabendazole-resistance in Ostertagia spp.

Three years after the start of an experiment to assess the merits of thiabendazole (TBZ) treatment of trichostrongylid parasites in weaner sheep, field isolates of Ostertagia spp and Trichostrongylus spp were made from weaner sheep treated under one of three treatment schemes. Treatment frequencies were "nil", "planned" (5 or 6/year) and "regular" (every 3 weeks). In addition an isolate was taken from a group of "tracer" sheep drenched with TBZ every 10 days. Resistance to TBZ was assessed using an in vitro egg hatch assay, pre- and post-treatment faecal egg counts and a controlled anthelmintic efficiency test. Pre- and post-treatment egg counts revealed the presence of TBZ-resistance in field isolates of mixed species. Egg hatch assays indicated a level of resistance for Ostertagia spp which was proportional to the frequency of TBZ treatment. The "planned", "regular" and "tracer" strains of Ostertagia spp had resistance ratios for eggs of 4, 13 and 15 respectively when compared to the "nil" strain. In the anthelmintic efficiency assay treatment with 44 mg kg-1 and 88 mg kg-1 of TBZ removed 82 and 96% respectively of the total Ostertagia burden (adults and larvae) from the "nil" strain and 30 and 75% respectively from the "planned" strain. The same dose rates against the "regular" and "tracer" strains and additional rates of 132 or 176 mg kg-1 against the "tracer" strain failed to reduce the Ostertagia burden significantly. Intestinal Trichostrongylus spp from all isolates were fully susceptible to TBZ at 44 mg kg-1. Levamisole at 7.0 mg kg-1 was highly effective (99% reduction) against the "tracer" strain of Ostertagia.