In the asphalt pavement with semi-rigid base, the shrinkage cracks initiating from base course often propagate upward to surface course to develops into reflective cracks. The fiberglass grid, as a woven geotextile, is widely used to retard the occurrence of reflective cracks in China, but the impacts of material composition and knitting methods of it on reflective cracks are still not clear. In this paper, two kinds of models, including a two-scale model of fiber bundle-fiberglass grids layer and a coupled thermo-mechanical model of pavement structure, are established. In the two-scale model, the elastic constants (ECs) of fiber bundle and fiberglass grids layer are calculated under different compositions and knitting methods and then assigned to the coupled thermo-mechanical model to estimate the fatigue lives at initiation phase (FL-IP) and propagation phase (FL-PP) of reflective cracks based on an estimation theory. Moreover, this study designs the laboratory tests to validate this estimation method of fatigue life. Then, based on the predicted fatigue life at different phases of crack progression, the sensitivity analysis is implemented to analyze the impacts of composition and knitting methods on the fatigue life. As a result, the recommendations on design and preparation of fiberglass grid are proposed. The results show that, in material composition, the glass fibers with high elastic modulus and volume fraction and the coating materials with high elastic modulus are beneficial to retarding the initiation and propagation of reflective cracks and in knitting methods, increasing density of fiber bundles parallel to the traffic direction and reducing braided angle also has positive effect. These results can be used to optimizing design and preparation of fiberglass grid to reduce the occurrence of reflective cracks in asphalt pavement.