Peninsular Malaysia Research Articles

Unveiling prey preferences of endangered wild Malayan tiger, Panthera tigris jacksoni, in Peninsular Malaysia through scat analysis via COI DNA metabarcoding

Understanding the prey preference of Malayan tiger (Panthera tigris jacksoni) in Malaysia is important to guide conservation planning initiatives. The utilisation of DNA metabarcoding provides valuable insights, particularly in the field of carnivora diet research. This technique has been proven to be effective for identifying various species within complex mixtures such as scat materials, where visual identification is challenging. The Cytochrome c oxidase subunit I (COI) locus has been selected as it is a widely used as an effective non-invasive approach for diet studies. Hence, given this advance approach, Malayan tiger scats were collected on the basis of existing records of their presence in two types of habitats, namely, protected areas (PA) and human–tiger conflict (HTC) areas. This study aimed to identify prey species in Peninsular Malaysia, based on Malayan tiger scat samples using DNA metabarcoding. Based on the partial mitochondrial COI region, DNA metabarcoding led to the taxonomic resolution of prey DNA remnants in scats and the identification of prey species consumed by Malayan tiger, which were predominately small-to-medium-sized prey, including livestock. The dominant DNA prey detected belongs to the family Canidae, followed by Bovidae, Vespertilionidae, Homonidae, Felidae, Phasianidae and Muridae. A significant difference (p < 0.05) was observed in alpha and beta diversity using the Shannon index and PERMANOVA with regard to prey richness and evenness in two different habitat groups, namely, PA and HTC. Our finding provides insights into Malayan tiger dietary requirements, which can be used to develop conservation plans and strategies for Malayan tiger, particularly for habitat priorities.

Companion to R. H. Beddom's Handbook to the Ferns of British India, Ceylon and the Malay Peninsula

No Abstract.

Third List of Species and Genera of Indian Phanerogams not Included in J. D. Hooker's Flora of British India (Excluding Bangladesh, Burma, Ceylon, Malayan Peninsula and Pakistan)

Calder et al. (1926) presented the first list of Phancrogams not included in J.D. Hooker's Flora of British India and it covered a span of twenty six years between 1898-1924. Razi (1959) enumerated the second list of Indian Phanerogams supplementing Calder's list for the period from 1924 to 1950 and this again covered a period of twenty six years. The third list now presented by the authors spanned a period of twenty five years (1950-1975) and the plants enumerated is geographically delimited to India, Bhutan and Nepal. In the present list the authors have listed about 1000 taxa of Phanerogams added to the Indian flora supplementing the lists of Calder (1926) and Razi (1959). The countries Bhutan and Nepal are part of Himalayan region and for any study of Himalayan flora of India, it is necessary to understand the floristic elements of Nepal and Bhutan since many Himalayan elements occur in the contiguous territories of India, Bhutan and Nepal.

Genetic erosion in a tropical tree species demonstrates the need to conserve wide-ranging germplasm amid extreme habitat fragmentation

Comparing the genetic diversity across different generations within tropical tree populations is an understudied topic. To assess the potential genetic consequences and conservation implications of contemporary disturbances, a population genomic study of Palaquium obovatum across age classes was undertaken. Trees and juveniles were sampled from ten different localities (eight in Singapore, two in Peninsular Malaysia) and subjected to double digest restriction-site associated DNA-sequencing (ddRAD-seq) to assess intergenerational genetic differences and investigate population structure in a hexaploid lineage. Genetic erosion, characterised by reduced heterozygosity, was found to have occurred in almost all wild populations over time, the exceptions being in one isolated coastal population and some areas with cultivated occurences. Population structure was highly localised with the number of genetically distinct populations usually following geographically separated districts, which indicates limitations in pollen and seed dispersal between fragments, possibly due to declines in the associated assemblage of dispersers. For this reason, the germplasm for conserving species diversity in degraded habitats and forest fragments should be selected from a wide range of wild populations across the landscape.

Growth Patterns, Status of Well-Being and Trend Analysis of Three Commercially Important Clupeid Species from Selangor Landing Port

Length-weight relationships (LWRs) and condition factors are vital to quickly understanding the fitness and well-being of fish. There is currently no available evidence on LWRs, condition factors and landing trend for these three species and an absence of this information will result in improper management and monitoring of fish species. In this study, the growth patterns, status of well-being and trend analysis of commercially important clupeid species from the west coast of Peninsular Malaysia were studied. Sardinella lemuru, Amblygaster sirm and Nematalosa japonica were selected due to high commercial value and conservation status. S. lemuru was specifically categorized as near threatened by the IUCN with decreasing global populations. Total length (cm) and body weight (g) of S. lemuru, A. sirm and N. japonica were accounted for in this study, which was obtained from the wet market in the west coast of Peninsular Malaysia. The relationships between length and, weight and, condition factors (Fulton’s, KF; relative condition factor, Kn; Allometric) were assessed. The allometric equation; W = aLb was used to evaluate the length-weight relationships while KF = 100W/Lb and Kn = W/aLb were used to estimate fish condition factor. ARIMA (1,1,1) and ARIMA (0,1,0) models were utilised to forecast the fish landing pattern until 2030. The results showed that the coefficient of determination R2 values varied between 0.263 (Sardinella lemuru), 0.5187 (Amblygaster sirm) and 0.8443 (Nematalosa japonica) and b values ranged from 0.0959 to 2.1005. From this, all three fish species have shown a negative allometric growth pattern b<3, reflecting a relatively slow growth rate and size of fish. No positive allometry was detected for any of the sampled fish species. A low value of relative condition factor (Kn < 1) suggests that all the fish species in this study have poor physical characteristics due to inadequate food resources, and unsuitable habitats for expansion of the fish population. Prediction based on Autoregressive Integrated Moving Average (ARIMA) model showed that N. japonica would increase gradually in the coming years, while S. lemuru would remain constant. This study contributes to the knowledge base for S. lemuru, A. sirm and N. japonica in the west coast of Peninsular Malaysia, and is crucial in terms of understanding the ecology of the habitat for supporting conservation strategies, and the restoration and management of these species in future.

Green hydrogen prospects in Peninsular Malaysia: a techno-economic analysis via Monte Carlo simulations

According to Malaysia's National Energy Transition Roadmap, hydrogen is a critical component of the country's energy transition. However, there is a scarcity of hydrogen studies for Peninsular Malaysian states, which limits discussions on green hydrogen production. This study employs a Monte Carlo model to assess the economic and technical factors influencing the success of green hydrogen in Peninsular Malaysia. The study focuses on three target years: 2023, 2030, and 2050, representing various stages of technological development and market adoption. The levelized cost of hydrogen (LCOH) of a 1-MW Proton Exchange Membrane (PEM) electrolyzer system ranges from $5.39 to $10.97 per kg in 2023, highlighting early-stage challenges and uncertainties. A 6-MW PEM electrolyzer system could achieve an LCOH of $3.50 to $4.72 per kg by 2030, indicating better prospects. Because of technological advancements and cost reductions, a 20-MW PEM electrolyzer system could achieve an LCOH of $3.12 to $3.64 per kg in 2050. The findings indicate that the northern regions of Peninsular Malaysia have consistently low LCOH values due to favorable geographical conditions. Due to minor variations in solar capacity factors, uncertainty distributions in LCOH remain stable across different regions. Some states may face increased uncertainty, emphasizing the need for additional policy support mechanisms to mitigate risks associated with green hydrogen investments. The sensitivity analysis shows that key cost drivers are shifting, with early-stage electrolyzer investments dominating in 2023 and electricity prices becoming more important in 2030 and 2050. Future research could focus on optimizing green hydrogen systems for areas with underdeveloped green hydrogen industries. This study contributes to informed discussions about green hydrogen production by emphasizing the importance of tailored strategies that consider local conditions and highlighting the role of Peninsular Malaysia in the energy transition.

Spatiotemporal Characteristics and Rainfall Thresholds of Geological Landslide Disasters in ASEAN Countries

Spatiotemporal Characteristics and Rainfall Thresholds of Geological Landslide Disasters in ASEAN Countries

Diversity and assemblage patterns of ladybirds (Coccinellidae) in different crop management practices in Peninsular Malaysia

Diversity and assemblage patterns of ladybirds (Coccinellidae) in different crop management practices in Peninsular Malaysia

The Issues and Challenges of Diversification Strategy in Multiple Industries: The Case of Kental Bina Sdn. Bhd.

A diversification strategy is one of the means to expand business among corporations, including Small and Medium Enterprises (SMEs). In Malaysia, many successful stories of SMEs’ diversification strategies permit others to follow in their footstep. The success of the strategy allows businesses to develop not only locally but also venture into the international market. However, various issues and challenges could be associated with diversification strategy implementation particularly among SMEs within multiple industries involved. Therefore, this study aims to discover the issues and challenges faced by Kental Bina Sdn. Bhd., one of the steadily growing diversified businesses in Northern Peninsular Malaysia. This study employed a few rounds of one-on-one interviews with the business owner as the key informant to meet the study objective. The recorded interview sessions are then transcribed and analyzed using thematic analysis through the Nvivo12 software. From the policy viewpoint, this study sheds light on the development of entrepreneurial training programs. The training program conducted by the relevant ministries and government agencies, including the Ministry of Entrepreneur and Cooperatives Development (MECD), Majlis Amanah Rakyat (MARA), SME Corporation (SMECorp.), and Tekun Nasional (TEKUN), needs to be specifically designed to address the issues and challenges of business diversification particularly among SMEs. For practical implication, this study helps to offer important information and inputs among other businesses, particularly SMEs, on what to expect when starting their diversification planning and strategy.

Feasibility Study of Solar-Powered Electric Vehicle Charging Infrastructure at Selected Petrol Stations in Malaysia

The transition from the conventional internal combustion engine (ICE) to electric vehicles (EV) are expected to result in higher total electricity demand in the country. With the introduction of more EVs into the market, the need for charging stations will grow, subsequently increasing power consumption. This research study aims to assess the potential of solar energy production at petrol stations in central region of Peninsular Malaysia and assess the feasibility of solar-powered petrol stations in meeting the energy demands of electric vehicles. 10 samples of existing petrol stations are selected to install a solar-powered DC charging station as a case study. To study their energy consumption, 90kW DC charger was evaluated. The design of the PV systems for the petrol stations was done by using the PVsyst simulation software. As a result, it is found that 90% of the designed PV systems are able to generate enough energy to meet the estimated annual energy consumption of the 90kW DC charging system. The results also showed that the cost to install the solar-powered charging system for both power ratings can be recouped in less than 2 years. Overall, this research study offers valuable insights for addressing the rising energy consumption resulting from the increased penetration of electric vehicles in the future. Additionally, the implementation of solar-powered charging infrastructure aligns with the country’s renewable energy objectives and efforts to reduce greenhouse gas emissions. However, the assessment of EV penetration and the adoption of charging infrastructure was conducted based on government targets rather than relying on current data regarding the actual numbers and trends of EVs and chargers in Malaysia. The availability of accurate and up-to-date data on the number of EVs, their battery characteristics, chargers, and user charging behaviors in Malaysia would contribute to further enhancing this research work.

Pathogenic variations in Colletotrichum spp. causing chilli anthracnose in Peninsular Malaysia

AbstractIn Peninsular Malaysia, Colletotrichum fioriniae, Colletotrichum fructicola, Colletotrichum scovillei, Colletotrichum siamense and Colletotrichum truncatum are the causative pathogens of chilli anthracnose. In the present study, the pathogenic variability and virulence levels of these five Colletotrichum spp. were evaluated. Isolates of C. fioriniae, C. fructicola, C. scovillei, C. siamense and C. truncatum were tested for pathogenicity using red and green Capsicum annuum and red and green Capsicum frutescens. Pathogenicity tests were conducted using spore suspensions and mycelial plugs on wounded and nonwounded chilli fruits. All isolates tested on the wounded chilli fruits caused anthracnose symptoms with variable disease severity and virulence. In nonwounded chilli fruits, only isolates of C. scovillei (n = 7) and C. truncatum (n = 5) caused infection. C. fioriniae, C. fructicola, C. scovillei, C. siamense and C. truncatum showed variability in pathogenicity and virulence levels in red and green chilli fruits of Ca. annuum and Ca. frutescens. Pathogenicity tests on wounded and nonwounded chilli fruits can be used to identify pathotypes within anthracnose pathogen populations. This is important for strategizing the effective disease management of chilli anthracnose.

Endogenous Endophthalmitis: A 10-Year Review of Cases on the East Coast of Malaysia.

Purpose Our study aimed to describe the clinical profile of endogenous endophthalmitis, focusing on patient demographics, infection sources, microbial profiles, clinical outcomes, and factors affecting the final visual outcome. Methods A retrospective review was performed on data from 68 eyes of 60 patients diagnosed with endogenous endophthalmitis and hospitalized in two tertiary hospitals on the East Coast of Peninsular Malaysia from January 2011 to December 2020. The analysis encompassed evaluating patient age, gender, laterality, risk factors, infection origins, presenting and final visual acuity, microbial results, treatment responses, and factors affecting final visual outcomes. Results The average age of the patient cohort was 54.9 ± 13.3 years. Females were more likely to have endogenous endophthalmitis than males (33, 55.0% vs. 27, 45.0%). Twenty-seven patients (45.0%) presented with endogenous endophthalmitis in the left eye, while 25 patients (41.7%) had it in the right eye, and eight patients (13.3%) had bilateral involvement. Most patients had underlying predisposing conditions, predominantly diabetes mellitus (53, 88.3%). Infection sources were identified in 42 patients (70.0%), out of which urinary tract infections account for the majority (11, 18.3%). Klebsiella species(14, 22.7%) were the leading pathogens and were significantly associated with liver abscess cases. In this series, the majority of patients had poor presenting and final visual acuity of worse than 3/60 (56, 82.4% and 53, 77.9%, respectively). Thirty-six eyes (52.9%) underwent vitrectomy, resulting in only four eyes (11.11%) achieving final visual acuity better than 6/12. Presenting visual acuity was identified as the factor contributing to the blind final visual outcome (r = 0.707, p < 0.001). Conclusion Females were found to be more commonly affected by endogenous endophthalmitis than males. Klebsiella species were the most commonly isolated microorganisms and were typically associated with liver abscesses. Urinary tract infection was the most common predisposing factor. A majority of the patients had poor presenting and final visual acuity, in which poor visual acuity is a significant indicator of blind visual outcomes.

A Single-Buyer Model of Imbalance Cost Pass-Through Pricing Forecasting in the Malaysian Electricity Supply Industry

The imbalance cost pass-through (ICPT) is a flexible component of the incentive-based regulation (IBR) that empowers power producers to adjust tariffs in response to variable fuel prices, thereby enhancing the economic resilience of electricity generation. In Malaysia, the Energy Commission has conducted biannual reviews of fuel and other generation costs. Any cost savings or increases identified during these reviews will be passed on to customers in the form of rebates or surcharges. Meanwhile, if an increment in the ICPT price signal can be provided to electricity providers and consumers, early preparation for operation budgeting can be realised, and energy management program development can be properly prepared. Due to this reason, this study proposes ICPT price forecasting for the electricity market in Peninsular Malaysia that will benefit the stakeholders. The study aims to construct an ICPT-related baseline model for the peninsular generation data by employing three forecasting methods. The forecasting performance is analysed using the mean absolute percentage error (MAPE). In light of our findings, the ARIMA method is one of the most accurate forecasting methods for fuel prices compared to the moving average (MA) and LSSVM methods. The observed price differences between the ARIMA and LSSVM models for ICPT are minimal. The ICPT price for July–December 2022 and January–June 2023 is MYR 0.21/kWh for the ARIMA and MYR 0.18/kWh for LSSVM, which are close to the actual TNB’s ICPT tariff. As for forecasting, the ICPT price is expected to drop in the next announcement. The findings of this study may have a positive impact on the sustainability of the energy sector in Malaysia.

Myxobolus acanthogobii Hoshina, 1952 and Myxobolus selari n. sp. (Myxosporea: Myxobolidae) infecting brain of commercial fishes in Terengganu, Malaysia.

Myxosporean infection in marine water fishes has drawn less attention than in freshwater fishes, which resulted in a higher taxonomic variety in freshwater in Malaysia. This study aimed to address the gap by conducting a myxosporean survey on two commercially significant marine fish species, Nemipterus furcosus (Valenciennes) (Eupercaria incertae sedis: Nemipteridae) and Selar crumenophthalmus (Bloch) (Carangiformes: Carangidae), collected from the northeastern part of peninsular Malaysia. During the examination of the organs, two distinct Myxobolus Bütschli, 1882 species were discovered in the brain tissue of these fishes, despite the absence of any observable pathological signs. The two Myxobolus species were characterized through morphometry, morphology, and analysis of partial small subunit ribosomal RNA (18S rDNA) gene. As a result, Myxobolus acanthogobii Hoshina, 1952, which infects 2.3% of N. furcosus, is synonymous with a myxobolid species commonly found in Japanese waters, based on its morphological traits, tissue tropism, and molecular diagnostics. Furthermore, a novel species, Myxobolus selari n. sp., was described, infecting the brain of one (11%) individual S. crumenophthalmus. This unique species displayed distinctive features, placing it within a well-supported subclade primarily comprising brain-infecting myxobolids. Maximum likelihood analysis further revealed the close relationships among these brain-infecting myxobolids, underscoring the significance of tissue tropism and host taxonomy for myxobolids. This study represents the initial documentation of Myxobolus species within the southern South China Sea, shedding light on the potential diversity of marine myxosporean in this region. This article was registered in the Official Register of Zoological Nomenclature (ZooBank) as urn:lsid:zoobank.org:pub:7C400E35-7CB8-4DEE-92B7-F75FF3926441.

Prioritising Important Plant Areas (IPAs) among the limestone karsts of Perak, Malaysia

SummaryThe flora of limestone karsts is exceptionally rich, due in particular to the wide array of microhabitats that limestone outcrops support. However, in Peninsular Malaysia limestone outcrops are heavily exploited for the quarry industry, cave temples, leisure venues and the development of geoparks, hence the limestone flora is amongst the most threatened biodiversity in the Peninsula. The 95 limestone karsts in Perak are currently known to support 538 species, representing c. 44.2% of the total limestone flora of Peninsular Malaysia. Of these, 13.2% (71 species) are endemic to the Peninsula of which 10 species are hyper-endemic (i.e. restricted to a single karst), 108 (20%) are threatened with extinction and 6 are considered likely to be Extinct. Typical of limestone in Peninsular Malaysia, a single karst harbours only a fraction of the total limestone flora such that a network of karsts is required to capture maximum plant diversity. Through evaluation of data from field surveys, herbarium specimens and the literature, 27 of the Perak karsts were found to trigger Important Plant Area (IPA) status, i.e. they harbour important populations of threatened species, outstanding botanical richness and/or are important examples of threatened habitats that in Perak include the foothills and limestone forest. Fifteen karsts were identified for priority conservation measures based on Conservation Priority Scores (CPS), a quantitative methodology based on endemic and threatened status. With the addition of two species-rich karst sites, 17 karsts are recommended as the first iteration of the network of priority IPA sites for Perak that together conserve 90% (483 species) of the Perak limestone plant diversity, 93% (66) of the endemic species and 94.4% (102) of the threatened species. CPS is found to be more reliable than using total species richness for assessing conservation importance and we also demonstrate that karst size and isolation are not primary indicators of range-restricted plant diversity. Significant knowledge gaps still exist, with 59 karsts in Perak without botanical surveys, and with many rare species having not been recollected for over 30 years, emphasising the need for further botanical research in these important and threatened landscapes.

Site occupancy of native terrestrial mammals in oil palm landscapes is driven by the synergy of land sparing and sharing strategies

The conversion of tropical rainforests into industrial oil palm monocultures has detrimental effects of local biodiversity. Although some medium-sized mammals have demonstrated a degree of adaptability in oil palm landscapes, the factors that influence their ability to persist in such human-altered environments remain obscure. Here, we investigated the site occupancy and activity pattern of the mammals in oil palm landscapes in Peninsular Malaysia. We deployed 81 camera traps throughout six oil palm cultivation areas with two distinct management styles (three sites each in organized smallholdings and industrial plantations). Smallholdings had higher site occupancy of native animal species than industrial plantations. The site occupancy of the mammals was influenced by the proximity to continuous forest, forest patches and riparian vegetation. Landscape metrics are characteristics that affect land sparing strategy, allowing wildanimals in agricultural landscapes to thrive in their habitat despite human threats. At stand-level, the detection of animals was determined by elevation, undergrowth vegetation cover/height, canopy cover, oil palm height, quantity of oil palm fruits, and prune stack thickness. Mammal occupancy was higher in the smallholdings due to the proximity to continuous forest. We recommend improving landscape heterogeneity and connectivity in oil palm matrix to promote species mobility and diversifying oil palm management away from monoculture farming. Our findings indicate that incorporating land sharing and sparing strategies into the oil palm matrix is necessary to conserve biodiversity and maintain ecological services. This implies that industry stakeholders ought not to disregard either land sparing or land sharing.

Data to the Malaysian Liverwort Flora, IV: a New Species of Mastigopelma Mitt. (Lepidoziaceae) from Cameron Highlands, Peninsular Malaysia

The new species, Mastigopelma latiffiana G. E. Lee, E. Pesiu et X. L. He, discovered in Pen-insular Malaysia, is described and illustrated as a new addition to the genus Mastigopelma Mitt., of the family Lepidoziaceae. The new species is corticolous, growing on a tree branch in a moist montane forest in Cameron Highlands, Pahang, at 1,900 m alt. It is characterised by the leafy shoots arising from stolons, branches, when present, all ventral-intercalary, and terminal branching lacking; asymmetrical leaves with almost straight ventral margins, rounded to truncate leaf apices, entire-margined leaves, thickened cell walls with nodular trigones, and glistening-homogeneous oil bodies (2–4 per cell); and retuse to blunt teethed underleaf apices. This discovery has increased the total number of known species of Mastigopelma Mitt. to five. An identification key to all the species of the genus is presented.

A high-resolution perspective of extreme rainfall and river flow under extreme climate change in Southeast Asia

Abstract. This article provides high-resolution information on the projected changes in annual extreme rainfall and high- and low-streamflow events over Southeast Asia under extreme climate change. The analysis was performed using the bias-corrected result of the High-Resolution Model Intercomparison Project (HighResMIP) multi-model experiment for the period 1971–2050. Eleven rainfall indices were calculated, along with streamflow simulation using the PCR-GLOBWB hydrological model. The historical period 1981–2010 and the near-future period 2021–2050 were considered for this analysis. Results indicate that, over former mainland Southeast Asia, Myanmar will face more challenges in the near future. The east coast of Myanmar will experience more extreme high-rainfall conditions, while northern Myanmar will have longer dry spells. Over the Indonesian maritime continent, Sumatra and Java will suffer from an increase in dry-spell length of up to 40 %, while the increase in extreme high rainfall will occur over Borneo and mountainous areas in Papua. Based on the streamflow analysis, the impact of climate change is more prominent in a low-flow event than in a high-flow event. The majority of rivers in the central Mekong catchment, Sumatra, Peninsular Malaysia, Borneo, and Java will experience more extreme low-flow events. More extreme dry conditions in the near future are also seen from the increasing probability of future low-flow occurrences, which reaches 101 % and 90 %, on average, over Sumatra and Java, respectively. In addition, based on our results over Java and Sumatra, we found that the changes in extreme high- and low-streamflow events are more pronounced in rivers with steep hydrographs (rivers where flash floods are easily triggered), while rivers with flat hydrographs have a higher risk in terms of the probability of low-flow change.

Understanding The Mimicker: Epidemiological Pattern and Determinant of Melioidosis Mortality in Negeri Sembilan, Malaysia.

Melioidosis, a tropical infectious disease caused by Burkholderia pseudomallei, is epidemic in most region in Southeast Asia with high case fatality. However, there is scanty information regarding the disease's epidemiological pattern, demographics, and underlying risk factors. This 5-year retrospective study of 185 confirmed cases which were taken from the Negeri Sembilan Melioidosis Registry between 2018 and 2022. We aim to describe the incidence, mortality rate, case fatality, relationship with meteorology, and factors that influence mortality in this central region of Peninsular Malaysia. Incidence rate (IR) of melioidosis in Negeri Sembilan is varied at 1.9 to 5.1 with mean of 3.1 in 100,000 population per year. IR varied between districts in the state from zero to 22.01 in 100,000 population per year. Mortality rate were ranged from 0.17 to 0.74 cases with mean of 0.44 cases in 100,000 population per year. The case fatality rate of this state scattered from 8.70% to 16.67%. There were no significant linear associations between cases and deaths with monthly rainfall and humidity. The mean age of patients was 52.8 years, predominated with age around 41-60 years old. Males (77.8%) predominated, and the majority of cases were Malays (88.9%) and had exposed to soil related activities (74.6%). Mortality from melioidosis was more likely in Bumiputera and non-Malaysians (p<0.05). Patients who had at least one comorbidity were at a higher risk of death from melioidosis (p<0.05). Diabetes mellitus was found in 41.1% of all identified cases, making it a major underlying risk factor for both developing and dying from melioidosis (aOR:19.32, 95%CI:1.91-195.59, p<0.05). Hypertension and mortality status in melioidosis are also significantly correlated (aOR: 7.75, 95% CI: 2.26-26.61, p<0.05). The epidemiological patterns of cases reported from Negeri Sembilan are consistent for the most part from previous studies in other states in Malaysia and global with regard to its incidence, case fatality, demographic and predisposing chronic diseases. Diabetes mellitus and hypertension were significantly linked to increased mortality among all determinants.

Spore Germination of Diplazium simplicivenium Holtt. (Athyriaceae) in Peninsular Malaysia

Diplazium simplicivenium Holtt. a species of fern in Peninsular Malaysia. This study reports an efficient method for D. simplicivenium spore sterilisation and the effect of plant growth regulators (PGRs) via green globular bodies (GGB). Sterilisation with 0.1% mercuric chloride effectively allowed spore germination. The result showed that the culture media supplemented with 0.5 mg/L gibberellin positively affects the weight of GGB and the number of shoots with significant differences (ANOVA, p &lt; 0.05). The soaking technique established in this study for spore sterilisation is an efficient approach, and the optimal plant and concentration of plant hormones were identified. This procedure can be applied to other indigenous ferns and closely similar species.