The textile and clothing (T&C) sector plays an important role in the growth and industrial development of emerging economies. Work stress is a real concern for organizations with no immunity for the T&C sector. It hampers individual as well as organizational work performance that ultimately places a significant hurdle in achieving competitiveness in the market. This study aims at exploring the antecedents and consequences of work stress and how different causes of stress influence perceived stress among the employees of the textile and clothing organizations in Pakistan. Data were collected from 1470 employees of 24 organizations. Cohen’s Perceived Stress Scale-10 (PSS-10) and two researcher-developed instruments (34 items and 22 items each) were used for the data collection. Information gathered was analyzed by PLS-SEM (partial least squares structural equation modelling) technique. Statistically significant results were found for the antecedents, namely, work-family conflict, role clarity, work characteristics, social working environment, managers’ support at work, and training and career development, with work-family conflict as the most significant, and for the consequences, namely, psychological, emotional, physical, performance, and behavioral with psychological effect as the most significant. The findings of the study could help managers, designers, planners, and ergonomists in making more informed and proactive decisions while attempting to prevent or control the stress phenomenon in the T&C sector. Moreover, the study highlights the need for gaining more in-depth insight into the work-stress phenomenon proactively so that the effectiveness of intervention strategies might be assured.