Test Material Research Articles

Effects of Anisotropic Mechanical Behavior on Nominal Moment Capability of 3D Printed Concrete Beam with Reinforcement

In this study, 3D-printed reinforced concrete beams were tested for flexural performance and compared with the analytical model based on the material test results. Two cementitious mixes (PSU and GCT) were designed for concrete printing and were mechanically tested and compared. Anisotropies in the compressive strength and modulus of elasticity of printed concrete were observed, applied to the analytical prediction of flexural bending behavior, and validated by actual test results. Significant differences between analytical predictions and experimental tests of the bending behaviors of the printed concrete beams were observed. Furthermore, higher compressive strengths and moduli of elasticity were observed when the loading direction was perpendicular to the printed layers or with the PSU mix. The effect of anisotropic mechanical properties on a reinforced beam was compared to the flexural bending tests for both mixes. The analytical model based on the material test results was compared to the flexural bending test results. The significant errors in the prediction of printed concrete’s structural performance, from 10% to 50%, suggest that factors other than reduced compressive strengths may influence the structural behaviors of printed concrete beams.

RC dapped-end beams with various reinforcement layouts: An experimental investigation

Reinforced Concrete (RC) discontinuity regions (D-regions), such as dapped ends, can represent vulnerable zones in existing RC structures since they can exhibit a brittle failure mechanism. In common design practice, the ultimate strength of D-regions is assessed through Strut and Tie (S&T) models. Current design codes recommend two resistant mechanisms for dapped ends but do not provide information about the identification of steel bars involved in the resistant mechanism when the reinforcement is formed by distributed bars. An experimental campaign on dapped-end beams with various reinforcement layouts, including configurations without diagonal bars or with one or multiple layers of diagonal bars, has been carried out at the Structures and Material Testing Laboratory of the Department of Civil and Environmental Engineering in Florence. Results allow for identifying the influence of different reinforcement layouts on the crack pattern at the Service Limit State (SLS) and on the post-peak response. Both the strain on bars and the crack opening displacements are investigated until failure. The work highlights that layouts with distributed diagonal bars reduce the crack widths at the SLS compared to other configurations, increase the ductility, and allow for the timely detection of damaged dapped ends during in situ inspections.

A-085 Comparing CO2 stability in patient samples and proficiency testing material

Abstract Background Total carbon dioxide (tCO2) measurements are important in the assessment of acid-base disorders. The collection and storage conditions of specimens are important preanalytical factors that can influence the accuracy of tCO2 measurements. Proficiency testing (PT) materials are used to assess the accuracy and reliability of a laboratory’s test performance. Unsuccessful performance on PT challenges can be an indicator of procedural issues. However, the stability of tCO2 in PT material compared to physiologic specimens is unknown and no guidance is provided to laboratories on how to handle PT samples for tCO2 measurement. This study aims to compare tCO2 stability in PT material with that of plasma samples when exposed to ambient air at room temperature. Methods Using enzymatic endpoint measurement on Vitros 4600 Chemistry System, tCO2 was measured in PT material (n = 5) and patient samples (n = 6) at baseline and after exposure to air for 15, 30, 60, 90, and 120 minutes. 500μL of each sample was aliquoted into EZ-nest tube adapters and baseline measurements were immediately analyzed. Average percent difference from baseline was calculated and >-10% bias was considered acceptable. Results The average percent difference from baseline in PT samples exceeded -10% as early as 30 minutes; whereas -3% bias was observed in patient samples at 60 minutes. Results are summarized in the table below. Conclusions The assessment of tCO2 stability ensures the reliability of laboratory measurements. Our results indicate tCO2 in PT material is not stable when exposed to air beyond 15 minutes, however tCO2 in most patient samples remained stable beyond 120 minutes. Understanding the stability limits of tCO2 in PT material allows us to devise workflows that prevent sample degradation during PT events. By establishing best practices for maintaining tCO2 integrity in PT material, laboratories can enhance the accuracy of proficiency testing results.

B-335 Analytical Performance of the Novel EQA Material for Fecal Immunochemical Test for Hemoglobin

Abstract Background EQA (external quality assurance) program for FIT (fecal immunochemical test for hemoglobin) is performed in many countries, and its demand increases as FIT is widely used in organized colorectal cancer screening programs. The materials used in FIT EQA vary from buffer-based liquid control, and lyophilized stool, to material that mimics stool, artificial stool. We could not find ideal EQA material with hemoglobin uniformly contained, good stability of hemoglobin, and ease in use such as ready-to-use form. The merit to use material that simulates actual sample, stool, is not only to be compliant with ISO15189 but also to suit various methods and sampling devices in FIT. The development of artificial stool material has been awaited by laboratories using FIT to evaluate errors and precisions in specimen collection.We assessed the analytical performance of the new artificial stool developed in HECTEF (Health Care Technology Foundation) to evaluate this artificial stool material was suitable as an EQA material. We examined the following three points: 1) Sample collection, 2) Uniformity, and 3) Stability of hemoglobin. Methods 1) Sample collection: The material was collected with the sampling probes of OC-Auto Sampling Bottle 3 (Eiken Chemical) and the collection amount was calculated by comparing the weight before and after sampling the artificial stool. 2) Uniformity: The uniformity of hemoglobin was evaluated during production before and after bottling based on “Uniformity of dosage units” section of The Japanese Pharmacopoeia 18th edition, 2021. The material was sampled from 10 different points and hemoglobin concentrations were measured using OC-Sensor DIANA. The uniformity of hemoglobin between the vials was evaluated by calculating the CV from the average of hemoglobin concentrations from samples of three points from three vials. 3) Stability of hemoglobin: The materials stored under different temperature conditions were compared with the material stored frozen at -20 °C. Storage conditions were at refrigeration for 20 months, at 30 °C for 1 month, and freeze-thawing for five times. Evaporation from the material was evaluated by weighing the samples before and after storage at 30 °C for 1 month. Results 1) Sample collection: The material was collected with sampling probes and the collection amounts were 106% (compared to theoretical amount).2) Uniformity: Uniformity of hemoglobin during production was within the specification of “Uniformity of dosage units” section of the pharmacopoeias, acceptance value<15.0. Uniformity of hemoglobin among three vials was within ±10% CV.3) Stability of hemoglobin: Stabilities of the material were all within ±10% from the control material (frozen) after storage at all temperature conditions. Evaporation from the material in a vial was below 1% at 30 °C storage for 1 month. Conclusions This artificial stool is suitable for EQA material because it is confirmed that it shows favorable analytical results in sample collection, uniformity, and stability of hemoglobin. The evaluation of this material in the EQA program to show its suitability was performed by Labquality. The result is reported in a poster at ADLM 2024.

A-059 Validation of self-collected capillary dried blood spot collection for clinical HbA1c testing

Abstract Background The increased prevalence of diabetes in socially disadvantaged populations requires solutions that are amenable to these communities. Unmanaged diabetes can cause cardiovascular disease, kidney damage, blindness, and limb amputation. Diabetes is traditionally diagnosed using fasting/oral glucose tolerance or hemoglobin A1c (HbA1c) tests; both of which require access to medical and laboratory facilities. Self-collected capillary dried blood spot (DBS) testing for HbA1c could alleviate health disparities by offering access to low cost and minimally invasive remote collection. The objective of this study was to validate self-collected capillary DBS specimens for clinical HbA1c testing. Methods DBS HbA1c was measured using the hemolysate application of the Roche cobas c513 Tina-quant Hemoglobin A1cDx Gen.3 assay. Calibration was performed according to the manufacturer’s package insert. Patient samples were prepared by collecting two 3mm DBS punches. Non-biological standards were prepared by placing two blank 3mm DBS punches into an empty tube and adding 10uL of standard. After 24 hours, samples were resuspended in 750uL of hemolyzing reagent and incubated for 75 minutes at room temperature (RT) while shaking at 800 RPM. Preliminary data showed that non-biological standards spotted onto DBS cards did not behave like real patient samples. A correction factor (CF) was derived (n=134) and challenged (n=20) to account for the systematic bias observed between matched patient DBS and venous specimens. Imprecision was established by running two patient DBS specimens as controls. Accuracy was evaluated using residual College of American Pathologists (CAP) proficiency testing (PT) material (n=32). Linearity was confirmed by running 5 levels of Maine Standards Linearity HbA1c Kit in triplicate. Stability of the DBS specimen and the hemolysate supernatant extracted from the DBS specimen were observed at RT. Results Specimens tested between venous and DBS HbA1c to derive the CF (n=134) had a bias of 0.26% (4.32%) and a linear equation of (y=0.938x +0.631; R=0.996). The resulting CF is: reported HbA1c% = (DBS HbA1c% - 0.631)/0.938. Matched venous and DBS specimens that challenged the CF (n=20) had a bias of -0.10% (-1.83%) and a linear equation of (y=1.06x -0.41; R=0.988). Imprecision was 2.3% and 1.3% for patient DBS control specimens with mean concentrations of 5.3% and 5.9%, respectively. Residual CAP PT samples (n=32) had an average bias of -0.05% (-0.40%). DBS HbA1c concentrations from 4.2-15.5% were linear (y=0.903x +1.15; R=0.999). The DBS specimen is stable for 60 days at RT and the hemolysate supernatant is stable for at least 10 hours at RT. Conclusions Self-collected capillary DBS specimens are acceptable for the clinical measurement of HbA1c, and effectively increase accessibility to diabetes screening and monitoring. Applying a CF to DBS patient samples removes the systematic bias observed between matched venous and DBS samples. Non-biological standards and capillary whole blood do not behave the same on DBS cards. Alternative PT for DBS specimens should be investigated as at-home collection advances.

Modification of activated carbon through chemical-physical activation method with KOH and 60Co gamma irradiation from banana leaf waste

Abstract Until now waste is still a problem in many big cities in Indonesia. In addition, the amount of waste increases as the population increases. Banana leaf waste is no exception, which is included in other types of waste, which accounts for 6.33% of the total waste in Indonesia in 2022. Utilization of banana leaf waste as a raw material for activated carbon production can also be an alternative to reducing the amount of wasted banana leaf waste because they contain various kinds of organic compounds. Activated carbon is widely used as an adsorbent in various sectors, especially in the industrial sector. Production of activated carbon must go through an activation process to enlarge the surface pores either chemically or physically, or even both. This study aims to compare the results of activated carbon surface area and functional groups using the chemical-physical activation method from banana leaf biomass raw materials with KOH and gamma irradiation at various doses. The research method used is an experimental method consisting of 4 stages: dehydration, carbonization, activation, and characterization. The research was started by dehydrating the leaves and continued with the carbonization stage at 350°C for 30 minutes. Carbon was divided into four samples that consisted of 1 blank and 3 test materials that activated with 30% (w/v) KOH solution for 24 hours and gamma-irradiated with a dose of 10, 30, and 50 kGy. Results were characterized using FTIR and BET. The effect of gamma 60Co irradiation on the activated carbon material from banana leaf waste can increase the peak transmittance value of the activated carbon functional group due to the formation and reaction of free radicals on its surface. Increase in the surface area (from 6.504 to 24.04 m2/g) is also associated with an increase in the absorbed dose due to the formation of more free radicals. However, the surface area obtained is not as expected due to the possibility of pore blockage and excessive activation time, which causes the surface area of the material to be small.

Characterization of mechanical and temperature effects on delamination and basic strength behaviors of PVC coated PUR foam substrate used in bus dashboard

This study focuses on the delamination and characterization of different loadings and temperature conditions of polyvinyl chloride (PVC)-coated polyurethane (PUR) foam component layers commonly used in bus dashboards. The method was developed through mechanical and thermal tests.The study involves the examination of primer and no primer PVC-coated PUR components using SEM (Scanning Electron Microscope), tensile-compression, three-point bending, primer characterization, peel, PUR material thermal expansion, and thermal tests. Physical and material tests were conducted to determine the mechanical properties of PVC and PUR foam and the material properties of the primer. Fourier transform infrared spectroscopy (FTIR) analysis was also conducted to identify the functional groups present in the PVC, PUR, and primer materials, helping to understand the material interactions.Some tests produced the expected results, while others did not. The primer characterization and three-point bending tests were unsuccessful due to sample foam breakage. However, tensile-compression tests, peel tests, thermal expansion tests, and thermal tests yielded the desired outcomes. During thermal testing of primed samples, it was observed that the samples shortened and bent as the primer material melted and began to separate from the PVC and PUR layers. This bending and swelling were attributed to the difference in thermal expansion coefficients between the melting primer material and the PVC and PUR layers. The results demonstrate that PVC-coated PUR components can be effectively separated, and they also highlight opportunities for design modifications to improve the durability of bus dashboards.

Techniques to mitigate membrane displacement for vacuum-membrane solar-dish facets

The prospects of a multifaceted vacuum-membrane solar dish concentrator are considered in this work. The membrane depths of these facets can shift slightly due to varying ambient conditions throughout an operational day, leading to major focal point shifts and a reduced overall efficiency. The purpose of this work was to experimentally investigate different methods of membrane displacement mitigation. A controlled-environment (indoor) enclosure was employed to examine the effects of static ambient conditions, allowing for the independent manipulation of the surrounding pressure and temperature. Various manufacturing techniques were also investigated within the controlled-environment enclosure, which included alterations in pretension, changes in membrane thickness and adjustments to overall facet sizes. Furthermore, outdoor tests were conducted to determine how solar radiation and convection affected membrane displacement as well as to investigate the performance of various membrane depth control strategies using an Arduino Uno microcontroller. The indoor results showed that opting for a small facet would minimize membrane displacement. The results were supported by material tests and a finite element analysis. The outdoor test results indicated that solar radiation and wind affected the internal temperature and consequently also affected the membrane depth. Furthermore, a focus control system maintaining a constant differential pressure across the membrane achieved the required accuracy of ±2 mm membrane displacement limitation. However, another focus control system consisting of a Hall effect module actively monitoring membrane depth emerged as the most effective, with an increase of about 0.09 mm and a decrease of approximately 0.02 mm from an initial depth of 10 mm. This level of stability with a focus control system will ensure that the facet maintains a consistent optical performance, ultimately advancing the reliability and efficiency of low-cost vacuum-membrane technology.

Strain hardening of acid-induced sodium caseinate gels as a function of pH and temperature

Strain hardening is a type of rheological behaviour that can be observed in large deformation tests of various materials. In oscillatory shear rheometry, it is characterised by an increase in storage and loss modulus ( and ) with increasing oscillation amplitude (i.e, shear strain) until the material fractures and the moduli drop again. Acid-induced caseinate gels were previously shown to exhibit such a strain hardening behaviour. However, it has not been studied in much detailed how the extent of strain hardening is affected by pH and acidification temperature. In this work, the strain hardening of acid-induced caseinate gels was characterised as a function of pH by starting strain sweeps at various time points during acidification with glucono-delta-lactone, and the experiments were conducted at either 20, 30 or 40 °C to investigate the impact of temperature. The results demonstrate that the strain at fracture and the extent by which increases with the strain amplitude change throughout acid-induced gelation of sodium caseinate in a complex manner with characteristic minima and maxima being barely related to the parameters observed in small strain experiments. The findings demonstrate the importance of considering the pH and temperature when characterising the strain hardening of caseinate gels as previous work showed that the strain hardening properties are related to water expression in forced syneresis experiments.

The Effect of Quenching Process on The Microstructure and Hardness of AISI 4140 Steel

Abstract Steel is a metal that has good strength widely used in industry, both in the construction and automotive fields as a protective material applied to the body of combat vehicles. Combat vehicle body materials must have high strength and hardness to withstand bullet penetration. Meanwhile, some types of steel do not have such strong point to endure impact and bullet penetration, so their strength and hardness need to be increased. One method that can be used to increase the qualities of steel is heat treatment, such as quenching process. In this research, the quenching process was carried out on commercial AISI 4140 steel to increase its hardness. The heat treatment process for AISI 4140 steel begins with an austenization process at a temperature of 850 °C with varying holding times, followed by a quenching stage using SAE 40 oil media. Afterward, the microstructure observation and hardness testing of the test material were carried out using the Optical microscope and Vickers method. The raw material has a hardness value of 215.58 VHN. After the heat treatment process in 30, 60, and 90 minutes was carried out, the hardness value of AISI 4140 steel reached 371.24, 474.48, and 545.5 VHN, respectively. The research results show that the longer the holding time in the quenching process lasts, the more increases the hardness value of the steel will be, which result in microstructure as a dominant martensite phase.

Experimental study of composite cold-formed steel and timber flooring systems with innovative shear connectors

An experimental investigation into the structural response of cold-formed steel-timber composite flooring systems with innovative and irregularly spaced shear connectors is presented in this paper. Five composite beam tests and a series of supporting material and push-out tests were carried out. The obtained results showed that the innovative shear connectors enabled the generation of considerable composite action, resulting in up to about 45% increases in load-carrying capacity and 15% and 20% increases in the initial and mid-range stiffnesses respectively over the non-composite system. Methods for predicting the effective flexural stiffness and moment capacity of the examined cold-formed steel-timber composite beams are presented and validated against the derived physical test data. It is shown that accurate predictions for both the flexural stiffness and moment capacity can be obtained, with mean prediction-to-test ratios of 0.93 and 0.91 respectively.

Short arm splints for wrist stabilization: A mechanical material test and cadaveric radiography study.

Documentation of the wrist stabilizing effect and mechanical properties of common splinting materials is warranted to support evidence-based condition-specific recommendations for wrist immobilization. The objectives of this study were to assess the wrist stabilizing properties of volar and dorsal short-arm splints made of four different materials and evaluate the mechanical properties of the splinting materials. Dorsal and volar short arm splints made of plaster of Paris (PoP) (eight layers), Woodcast (2 mm, rigid vented), X-lite (classic, two layers) or a 3D-printed material (polypropylene) were sequentially mounted on 10 cadaveric arm specimens and fixed in a radiolucent fixture. This enabled the evaluation of maximum wrist flexion and extension relative under an orthogonal load of 42 N via radiographic images. In addition, a three-point bending test was performed on ten sheet duplicates of each of the four splinting materials. When applied as a volar splint, PoP had the highest capability to resist wrist flexion and extension. However, when applied as a dorsal splint, Woodcast exhibited a lower wrist flexion and a similar wrist extension. The 3D-printed splints-both volar and dorsal-showed the highest mean wrist flexion and extension. The mechanical properties of the Woodcast, X-lite and 3D-printed splinting materials were very similar. PoP exhibited distinct properties with a stiffness of 146 (95% confidence interval [CI]: 120-173) N/mm and a deflection at F max of 0.6 (95% CI: 0.5-0.7) mm compared to ≤7.7 (95% CI: 7.4-7.9) N/mm and ≥20 (95% CI: 18-22) mm for the other materials. PoP displayed better wrist stabilizing properties and material stiffness than Woodcast, X-lite and 3D-printed polypropylene. When considering wrist stabilizing properties, PoP may still prove to be the preferred choice for wrist immobilization. Not applicable.

The impact of adjuvant antibiotic hydrogel application on the primary stability of uncemented hip stems

ObjectivesTo assess the effect of adjuvant antibiotic-loaded hydrogel application on the primary stability of implanted uncemented hip stems.DesignBiomechanical study.SettingAn electro-mechanic material test system (#5866, Instron, Norwood, MA, USA) equipped with...

Finite element modeling for cohesive/adhesive failure of adhesive structures with a thermosetting resin

In this study, we established a novel method based on the finite element method (FEM) for predicting the strength of adhesive structures. Viscoplasticity, void growth, and cohesive zone model were introduced into the FEM to create a nonlinear damage growth (NDG) model. This model was used to comprehensively analyze the process zones within the adhesive layer. Furthermore, the embedded process zone approach was used to develop an interface constitutive law that averages the mechanical response of the adhesive layer. This modified Ma–Kishimoto (MMK) model can accurately represent the adhesive layer as an interface element and is computationally efficient. Furthermore, the study obtained the necessary interface properties for the MMK model from the NDG model, creating a numerical material test that can approximate the effect of the process zone. To validate the proposed method, single-lap shear tests were performed, and the accuracy of the predicted strength and deformation field was evaluated. The damage evolution in the NDG model and the MMK model were compared, and the scope of application of the MMK model was discussed. The results of this study can be used as a reference for the failure mechanism of thermosetting adhesives and establishment of design indices for adhesive structural strength.

A Biomechanical Analysis of Oblique Metacarpal Metadiaphyseal Fracture Fixation in a Cadaver Model

Although metacarpal fractures are typically managed nonoperatively, when surgical management is indicated, metacarpal fractures are commonly treated with crossed Kirschner wires (K-wires), which may limit early range of motion. Intramedullary implants are increasing in use with the potential advantage of early range of motion; however, stability in oblique metacarpal neck fractures remains a theoretical concern. The purpose of this study was to determine the biomechanical stability of noncompressive intramedullary fixation for oblique metacarpal neck fractures compared with crossed K-wire fixation. The index, long, and small metacarpals were harvested from three matched pairs of fresh-frozen cadavers. Oblique fractures at the metadiaphyseal region were created in each metacarpal. Each metacarpal was randomized to noncompressive, threaded intramedullary nail fixation or fixation with two crossed K-wires. Specimens were mounted in a Materials Testing System load frame and axially loaded until failure. Load to failure (LTF), stiffness, and load to 2 mm displacement were calculated from load-displacement curves. Differences in peak LTF, stiffness, and load to 2 mm displacement between noncompressive intramedullary fixation and crossed K-wire fixation were evaluated. The noncompressive intramedullary fixation cohort had a significantly higher LTF (1,190.9 ± 534.7 N vs 297.0 ± 156.0 N) and stiffness (551.3 ± 164.6 N/mm vs 283.0 ± 194.5 N/mm) when compared with the crossed K-wire fixation cohort. Load at 2 mm displacement was greater in the noncompressive intramedullary fixation cohort compared with crossed K-wire fixation (820.5 ± 203.9 vs 514.1 ± 259.6). For oblique metadiaphyseal metacarpal fractures, noncompressive intramedullary fixation provides a biomechanically superior construct under axial loading in terms of LTF, stiffness, and load to 2 mm of displacement compared with crossed K-wire fixation. Noncompressive intramedullary nails may be an alternative to K-wire fixation for the treatment of oblique metadiaphyseal metacarpal fractures.

THE EFFECT OF DIFFERENT STAINING SOLUTIONS ON THE COLOR STABILITY OF PERMANENT INDIRECT COMPOSITE RESINS PRODUCED BY ADDITIVE AND SUBTRACTIVE TECHNIQUES

Objective: To investigate the effects of various beverages on the color stability of permanent composite resins produced by additive (AM) or subtractive manufacturing (SM) techniques comparatively. Materials and Methods: Six composite resin materials produced by SM (Vita Enamic-VE, Cerasmart-CE, Lava Ultimate-LU) and AM (Varseo Smile Crown plus-VSC, Saremco print Crowntech-SPC, Formlabs 3B Permanent Crown-FPC) techniques were selected and soaked in different solutions (artificial saliva, black tea, coffee) for different times (0, 1 and 7 days). L*, a*, b* values of the samples were recorded using a spectrophotometer. The color changes of the samples were determined using the CIELAB formula. In determining the color differences between the test materials, Kruskal-Wallis analysis was used when one-way analysis of variance wasn’t available. Results: Group VE was the least stained group on the 1st and 7th day of artificial saliva solution and the 7th day of coffee solution, while Group CE was the least stained group on the 1st day of coffee solution. In the tea solution, on the 1st and 7th days, there wasn’t difference in the materials' color change (p>0.05). Tea and coffee solutions caused statistically significantly more color change in all test materials than artificial saliva (except Group CE on the 7th day, Group VSC and FPC on the 1st day) (p<0.05). Conclusion: 3D permanent composite resins generally showed more staining than CAD/CAM milled composite resins. Tea and coffee staining solutions changed the color of the materials compared to artificial saliva. As the storage time increased, more color changes were observed.

Multimodal Non-Destructive In Situ Observation of Crystallinity Changes in High-Density Polyethylene Samples with Relation to Optical Parameters during Tensile Deformation

Abstract: Many non-destructive optical testing methods are currently used for material research, providing various information about material parameters. At RECENDT, a multimodal experimental setup has been designed that combines terahertz (THz) spectroscopy, optical coherence tomography (OCT), infrared (IR), and Raman spectroscopy with a tensile test stage. This setup aims to gather material information such as crystallinity and optical parameters of high-density polyethylene (HDPE) during a tensile test. The setup compares common IR and Raman spectroscopy and the less common optical methods THz and OCT. Complementarity is achieved through different frequency ranges and measurement approaches, resulting in different measured optical material parameters and depths. During tensile testing, HDPE samples with varying crystallinity were analysed, and the determined optical parameters such as refractive index, birefringence, scattering coefficient of decay, and penetration depth can be correlated with the change in crystallinity. These findings demonstrate that the optical methods and their outcomes can be interconnected. With further optimization of the experimental setup, it would be possible to observe the alignment of fibres in fibre composite panels and the stress distribution of polymers effectively. This opens interesting possibilities for polymer characterization in the future, including quality control during moulding processes and material testing.

Comparison of the Torsional Strength of Material Samples Made Using Selected Rapid Prototyping Methods

In recent years, there has been a marked increase in the use of rapid prototyping techniques that support industrial processes. The analysis of studies on strength tests of parts manufactured using additive techniques from model materials and hybrid composites usually includes tests of material and strength parameters based on uniaxial tension, bending, and compression tests. For elements manufactured from polymeric materials using traditional methods (e.g. injection moulding), data obtained from these tests are sufficient to determine the strength of the material and to apply the results in the product design process. In the case of the layered extrusion method, due to the model construction process, strength data based on standard samples do not have a direct transposition on the strength of machine parts operating under various load ranges, especially torsional loads. The publication presents the results of tests on the torsional strength of cylindrical samples with splines produced by layered extrusion, vacuum casting technology, and hybrid technology, which combines both technologies. The test samples were made using a Prusa i3 MK3 3D printer and the plasticised plastic modelling (fused filament fabrication, FFF) method, while PolyJet technology was used to create a reference model. The findings indicate that hybrid technology, in which a thin-walled mould is filled with a chemically hardened polymer, achieves the desired strength for the manufactured parts while maintaining dimensional accuracy and shape. The research results show that producing rotating elements using the hybrid technology reduces the sample core’s ability to undergo plastic deformation due to the adhesion between the materials and the occurrence of notches resulting from the model production process using the layered extrusion method.

Development of Risk-Based Methodologies for Highway Pavement Construction Inspection

Construction inspection plays a critical role in Portland cement concrete pavement (PCCP) and hot mix asphalt (HMA) pavement projects. To ensure the quality of pavement projects, state departments of transportation (DOTs) in the U.S. typically conduct construction material tests and inspect workmanship processes using quality assurance (QA) programs. Given today’s reality of reduced QA inspection resources such as funding, staff ability, and availability, several state DOTs have used a risk-based inspection (RBI) approach to optimize their QA processes. A thorough review of existing literature revealed limited studies examining RBI methodologies in highway pavement construction projects. The aim of this study is to investigate the use of RBI for pavement construction projects. It employed a triangulation research methodology, including an extensive literature review, a survey questionnaire of 50 state DOTs, focus groups, and a vetting workshop. A list of 16 core items in field inspection for both PCCP and HMA pavement projects was developed and verified. A generic risk rating of these critical inspection activities was determined. Additionally, inspection frequency, documentation effort, and inspector experience associated with these critical inspection activities identified through focus groups and the vetting workshop served as a reference point when conducting RBI for PCCP and HMA pavement projects. This study contributes to the body of knowledge and the highway construction industry by identifying and prioritizing 16 core inspection items for PCCP and HMA pavement construction. Construction project managers and inspectors may benefit from this study by analyzing the risks of core inspection items and implementing appropriate risk mitigation plans.

Molecular Biomarkers for In Vitro Thrombogenicity Assessment of Medical Device Materials.

To develop standardized in vitro thrombogenicity test methods for evaluating medical device materials, three platelet activation biomarkers, beta-thromboglobulin (β-TG), platelet factor 4 (PF4), soluble p-selectin (CD62P), and a plasma coagulation marker, thrombin-antithrombin complex (TAT), were investigated. Whole blood, drawn from six healthy human volunteers into Anticoagulant Citrate Dextrose Solution A was recalcified and heparinized over a concentration range of 0.5-1.5 U/mL. The blood was incubated with test materials with different thrombogenic potentials for 60 min at 37°C, using a 6 cm2/mL material surface area to blood volume ratio. After incubation, the blood platelet count was measured before centrifuging the blood to prepare platelet-poor plasma (PPP) and platelet-free plasma (PFP) for enzyme-linked immunosorbent assay analysis of the biomarkers. The results show that all four markers effectively differentiated the materials with different thrombogenic potentials at heparin concentrations from 1.0 to 1.5 U/mL. When a donor-specific heparin concentration (determined by activated clotting time) was used, the markers were able to differentiate materials consistently for blood from all the donors. Additionally, using PFP instead of PPP further improved the test method's ability to differentiate the thrombogenic materials from the negative control for β-TG and TAT. Moreover, the platelet activation markers were able to detect reversible platelet activation induced by adenosine diphosphate (ADP). In summary, all three platelet activation markers (β-TG, PF4, and CD62P) can distinguish thrombogenic potentials of different materials and detect ADP-induced reversible platelet activation. Test consistency and sensitivity can be enhanced by using a donor-specific heparin concentration and PFP. The same test conditions are applicable to the measurement of coagulation marker TAT.