Test Tank Research Articles

Therapeutic intervention of vitamin B12 in mitigating chronic alcoholism induced alterations in adult zebrafish (Danio rerio): a holistic in vivo approach

Background Chronic alcoholism refers to the unpleasant symptoms directly resulting from excessive drinking. Increased alcohol metabolites and an unbalanced oxidative state are likely to blame for the reported effects under these circumstances. According to preclinical and clinical research, vitamin B12 can act on several organ systems with demonstrated neuroprotective, antioxidant, and glutamate modulating properties. Objective This research sought to examine the ameliorative effects of vitamin B12 (VtB12) in persistent alcohol (AlOH) exposed adult zebrafish with the help of following parameters like the anxiety related behavior test, Oxidative stress, and antioxidant assays, histological and immunofluorescence analysis. Methods Zebrafish pretreated with 0.40% AlOH (v/v) for 120 min (+AlOH) or not (−AlOH), were exposed for 6 h to home tank water (−VtB12) or to 59 µg-VtB12/kg-fish food (+VtB12) to analyze anxiety behavior in the geotaxis (novel tank) test as well as the oxidative brain damage in the adult zebrafish. Results Adult zebrafish exposed to chronic AlOH showed a decrease in the distance travelled, average and mobility speed, and increased the average frozen time, the explored area, and total no. of the site explored in the trapezoid tank. AlOH exposure also resulted in oxidative damage, enhanced lipid peroxidation, advanced oxidative protein products, decreased enzymatic and non-enzymatic antioxidant activities, and enhanced reactive oxygen species generation. Additionally, VtB12 supplementation improved neurogenesis, evident in increased Nissl cell numbers and NeuN expression in the brain. Conclusion Chronic alcoholism may be effect on the brain cells as well as on the neuro-behavior of zebrafish. This research demonstrated that VtB12 shows promise as a neuroprotective agent against chronic alcoholism induced alterations in zebrafish’s brain.

Impacts of Environmental Concentrations of Nanoplastics on Zebrafish Neurobehavior and Reproductive Toxicity.

Nanoplastics, as emerging environmental pollutants, can transport contaminants across marine environments, polluting pristine ecosystems and being ingested by marine organisms. This transfer poses a severe threat to global aquatic ecosystems and potentially impacts human health through the food chain. Neurobehavioral and reproductive toxicity are critical areas of concern because they directly affect the survival, health, and population dynamics of aquatic species, which can have cascading effects on the entire ecosystem. Using zebrafish as a model organism, we investigated the toxic effects of environmental concentrations of polystyrene nanoplastics (PS-NPs). Behavioral assessments, including the novel tank test and open field test, demonstrated significant neurobehavioral changes, indicating increased anxiety and depressive behaviors. A pathological analysis of brain and gonadal tissues, along with evaluations of neurobehavioral and reproductive toxicity biomarkers, revealed that exposure to PS-NPs leads to brain tissue lesions, inflammatory responses, oxidative stress activation, hormone level disruptions, and gonadal damage. Real-time quantitative PCR studies of reproductive gene expression further showed that PS-NPs disrupt the endocrine regulation pathways of the brain-pituitary-gonadal (BPG) axis, causing reproductive toxicity with sex-specific differences. These findings provide crucial insights into the impacts of nanoplastics on aquatic organisms and their ecological risks, offering theoretical support for future environmental protection and pollutant management efforts.

Whole-brain mapping in adult zebrafish and identification of a novel tank test functional connectome.

Identifying general principles of brain function requires the study of structure-function relationships in a variety of species. Zebrafish have recently gained prominence as a model organism in neuroscience, yielding important insights into vertebrate brain function. Although methods have been developed for mapping neural activity in larval animals, we lack similar techniques for adult zebrafish that have the advantage of a fully developed neuroanatomy and larger behavioral repertoire. Here, we describe a pipeline built around open-source tools for whole-brain activity mapping in freely swimming adult zebrafish. Our pipeline combines recent advances in histology, microscopy, and machine learning to capture cfos activity across the entirety of the adult brain. Images captured using light-sheet microscopy are registered to the recently created adult zebrafish brain atlas (AZBA) for automated segmentation using advanced normalization tools (ANTs). We used our pipeline to measure brain activity after zebrafish were subject to the novel tank test. We found that cfos levels peaked 15 minutes following behavior and that several regions containing serotoninergic, dopaminergic, noradrenergic, and cholinergic neurons were active during exploration. Finally, we generated a novel tank test functional connectome. Functional network analysis revealed that several regions of the medial ventral telencephalon form a cohesive sub-network during exploration. We also found that the anterior portion of the parvocellular preoptic nucleus (PPa) serves as a key connection between the ventral telencephalon and many other parts of the brain. Taken together, our work enables whole-brain activity mapping in adult zebrafish for the first time while providing insight into neural basis for the novel tank test.

Comparative study of dynamic characteristics between a FHAWT and a helical type FVAWT

Floating offshore wind turbines (FOWTs) are a growing area of interest, with significant ongoing research work and commercial development. There are two types of FOWT, namely floating horizontal axis wind turbine (FHAWT) and floating vertical axis wind turbine (FVAWT). It has been a long-standing debate on which type of FOWT has better technical and economic performance. However, the research work on comparative study of these two types of FOWT is very rare. In this study, we aim to systematically evaluate the performance of FHAWT and FVAWT, with particular focus on comparing their dynamic characteristics by using coupled numerical models. Tank testing for these two types of FOWT was conducted at the wave basin, and a series of code-to-experiment comparisons were carried out to validate the numerical models. Afterwards, dynamic characteristics, including the control strategy, aerodynamic power, floater motions, tower base bending moments, and blade deformations, are compared between the FHAWT and helical type FVAWT. Results show that the FHAWT presents better performance on the aerodynamic performance and tower base bending moments, while the helical type FVAWT has the advantage of minimal blade deformations.

Application of Casein Micelles for Targeting Huntington's Disease in Experimental Zebrafish Model.

Huntington's disease (HD) is an incorrigible neuropsychiatric disorder with reduced cognition and motor abnormalities. Piperine (PIP) is an alkaloid with antioxidant, anti-inflammatory, and neuroprotective activities; however, poor therapeutic efficacy limits its further use. The current study focuses on the enhanced therapeutic potential of PIP@CM against an experimental zebrafish model of HD. PIP@CM was fabricated using spray drying technology, followed by solid-state investigations. We performed in vitro release and in vitro antioxidant activity (DPPH assay) of PIP and PIP@CMs. In addition, in vivo studies were conducted on zebrafish using 3-nitropropionic acid (3-NPA) (60mg/kg) as a neurotoxin and treated with PIP (5mg/kg) and PIP@CM (25mg/kg equivalent to 5mg/kg PIP). After dosing, various in vivo studies (behavioral, biochemical, and histological) were conducted. The solid-state characterization techniques revealed the loss of crystallinity after micelles formation. In vitro release and antioxidant assays showed higher release and enhanced activity of PIP@CM. In vivo studies revealed that 3-NPA administration causes HD, as evidenced by the results of open field test (OFT) and novel tank diving test (NTD) tests. Moreover, 3-NPA causes an increase in oxidative stress, as confirmed by biochemical and histopathological studies. PIP@CM treatment significantly improved behavioral performance in OFT and NTD tests and reduced oxidative stress markers as compared to pure PIP and untreated HD model.

Spatial Localization of Transformer Inspection Robot Based on Adaptive Denoising and SCOT-β Generalized Cross-Correlation.

In the detection process of the internal defects of large oil-immersed transformers, due to the huge size of large transformers and metal-enclosed structures, the positional localization of miniature inspection robots inside the transformer faces great difficulties. To address this problem, this paper proposes a three-dimensional positional localization method based on adaptive denoising and the SCOT weighting function with the addition of the exponent β (SCOT-β) generalized cross-correlation for L-type ultrasonic arrays of transformer internal inspection robots. Aiming at the strong noise interference in the field, the original signal is decomposed by an improved Empirical Mode Decomposition (EMD) method, and the optimal center frequency and bandwidth of each mode are adaptively searched. By extracting the modes in the frequency band of the positional localization signal, suppressing the modes in the noise frequency band, and reconstructing the Intrinsic Mode Function (IMF) of the independently selected superior modal components, a signal with a high signal-to-noise ratio is obtained. In addition, for the traditional mutual correlation algorithm with a large delay estimation error at a low signal-to-noise ratio, this paper adopts an improved generalized joint weighting function, SCOT-β, which improves the anti-jamming ability of the generalized mutual correlation method at a low signal-to-noise ratio by adding an exponential function to the denominator term of the SCOT weighting function's generalized cross-correlation. Finally, the accurate positional localization of the transformer internal inspection robot is realized based on the quadratic L-array and search-based maximum likelihood estimation method. Simulation and experimental results show the following: the improved EMD denoising method better improves the signal-to-noise ratio of the positional localization signal with a lower distortion rate; in the transformer test tank, which is 120 cm in length, 100 cm in width, and 100 cm in height, based on the positional localization method in this paper, the average relative positional localization error of the transformer internal inspection robot in three-dimensional space is 2.27%, and the maximum positional localization error is less than 2 cm, which meets the requirements of engineering positional localization.

Towards a reliable method for extrapolation of propulsion performance for vessels with twin-crp-pod system

The study presents power performance prediction of an Ultra Large Container Ship (ULCS) with hybrid twin-crp-pod propulsion system. Twin crp-pod propulsion system is a combination of three concepts: twin screw, contra-rotating propellers (crp) and conventional shaft propellers with pod propulsors behind. The presented study shows the current extrapolation method for crp propulsion systems and tries to point out its weaknesses. As a case study, a 400 m ULCS has been investigated in full-scale and in model scales of 24 and 37.416. The analyses were carried out for all scales with use of CFD numerical methods and for the scale of 37.416 based on towing tank tests. All the results have been extrapolated with the same method and results have been compared. The investigations clearly show differences in delivered power prediction extrapolated from towing tank results giving the maximum value and from CFD made to scale of 24 the minimum value. Finally, conclusions on possible sources of differences, including the numerical and analytical methods are presented.

Evaluation of behavioral and neurochemical changes induced by carbofuran in zebrafish (Danio rerio)

Carbofuran (CF) is a carbamate class pesticide, widely used in agriculture for pest control in crops. This pesticide has high toxicity in non-target organisms, and its presence in the environment poses a threat to the ecosystem. Research has revealed that this pesticide acts as an inhibitor of acetylcholinesterase (AChE), inducing an accumulation of acetylcholine in the brain. Nonetheless, our understanding of CF impact on the central nervous system remains elusive. Therefore, this study explored how CF influences behavioral and neurochemical outcomes in adult zebrafish. The animals underwent a 96-hour exposure protocol to different concentrations of CF (5, 50, and 500 μg/L) and were subjected to the novel tank (NTT) and social preference tests (SPT). Subsequently, they were euthanized, and their brains were extracted to evaluate neurochemical markers associated with oxidative stress and AChE levels. In the NTT and SPT, CF did not alter the evaluated behavioral parameters. Furthermore, CF did not affect the levels of AChE, non-protein sulfhydryl groups, and thiobarbituric acid reactive species in the zebrafish brain. Nevertheless, further investigation is required to explore the effects of environmental exposure to this compound on non-target organisms.

MMG 3DOF model identification with uncertainty of observation and hydrodynamic maneuvering coefficients using MCMC method

The trajectory prediction using ship maneuverability mathematical models is one of the essential technologies implemented in autonomous surface ship. Several ship maneuverability mathematical models and each one with a particular hydrodynamic coefficient approximation using towing tank tests are existed. However, it is presented difficult to directly inverse estimate the hydrodynamic maneuvering coefficients of a ship maneuverability mathematical model from operational data consisting of ship trajectory and maneuvering operation records. This paper proposed a method for estimating the hydrodynamic maneuvering coefficients of the MMG 3DOF model using three types of time-series ship motions (surge, sway, and yaw velocity) as observed data. In the assumption of this paper, there is uncertainty in observations and the hydrodynamic maneuvering coefficients of the MMG 3DOF model. The proposed method outputs samples of the simultaneous posterior probability distribution of the hydrodynamic maneuvering coefficients by the MCMC method using the observed data and stochastic model. A robust trajectory with a wide range can be presented by conducting ship maneuvering simulations using these samples. To verify the feasibility of the proposed method, this paper conducted observation system simulation experiments (OSSE) using the KVLCC2 L7 model and applied the proposed method to several free-running model ship tests. Results showed that on the assumption that MMG 3DOF model can explain the ship's state and trajectory in real world, the proposed method can estimate the ship hydrodynamic maneuvering coefficients of the MMG 3DOF model corresponding to the observed ship trajectory and control data including the error of observed data.

Sedative effect of U-49900 (3,4-dichloro-N- (2-(diethylamino)cyclohexyl)-N-methyl-benzamide) in adult Zebrafish

BACKGROUND:U-49900 is a chemical analog of U-47700. Despite the growing abuse of synthetic opioids, including U-49900, its psychopharmacological and toxicological profiles remain poorly understood. The zebrafish (Danio rerio) is a promising model organism in neuroscience often used for the psychopharmacological evaluation of neurotropic drugs. OBJECTIVE:To evaluate the effects of U-49900 on the behavior of adult zebrafish in a new tank. MATERIALS AND METHODS:The effect of the test compound U-49900 at 1, 5, 10, and 25 mg/L concentrations (incubation for 20 minutes) on the behavior of adult zebrafish was assessed using the “new aquarium” test. U-49900 is 3.4-dichloro-N-(2-(diethylamino)cyclohexyl)-N-methylbenzamide and is considered an analog of the synthetic opioid U-47700, causing withdrawal in humans. RESULTS:The psychopharmacological profile of U-49900 at 1, 5, 10, and 25 mg/l concentrations (water immersion) in adult zebrafish was evaluated with the novel tank test. U-49900 at 25 mg/l for 20 min reduced the distance traveled and number of top entries in the novel tank test (p 0.001 vs. control group), but unaltered other parameters.CONCLUSIONS:Overall, the sensitivity of zebrafish to synthetic non-fentanyl opioids such as U-49900 indicates the possibility of developing high-throughput screening platforms for finding effective therapies for pathological conditions caused by synthetic opioids.

Multidimensional hybrid software-in-the-loop modeling approach for experimental analysis of a floating offshore wind turbine in wave tank experiments

A hybrid experimental modeling approach for floating offshore wind turbines (FOWTs) in a wave tank is presented. The method called real-time hybrid method (RTHM) or software-in-the-loop (SIL) includes physically reproduced hydrodynamic loads in the wave tank, with a Froude-scaled model while aerodynamic loads are reproduced on the model by an actuator. The force is applied on the tower top and calculated by a numerical simulation running in real-time as a function of the measured velocity and position of the physical model. Couplings between the platform motion, the aerodynamic loads and the wind turbine controller are hence accounted for. A new SIL actuator enabling the reproduction of a multi-component force at the tower top of an experimental scaled wind turbine model is developed, and the quantification of the accuracy of the replicated forces is studied. The test case includes a floating spar platform carrying a 10 MW horizontal axis wind turbine. The results show that this SIL actuator can accurately replicate the low and wave-frequency aerodynamic loads, which are essential for wave tank testing. Lastly, a case study of the wind turbine model in colinear and misaligned wind and wave conditions is presented, showing 3D forces and induced motions.

Dynamic motion analysis of stepless and stepped planing hulls in random waves: A CFD model perspective

Predicting the dynamic responses of planing hulls in real sea conditions is important for identifying how basic design factors influence their seakeeping performance. Hence, there is a pressing need to provide high-fidelity models for predicting the motions of these hulls in random waves, representing actual seas. In this article, a computational-based model for solving viscous fluid flow around the vessel is built to address this problem. Three different planing hulls, denoted as C, C1, and C2, each distinguished by the number of steps incorporated on their bottom surfaces (1 and 2 indicating the respective step count, with case C being the stepless hull), are modeled in a Computational Fluid Dynamics (CFD) tank, allowing for analysis of the effects of steps on dynamic responses of a planing surface operating in random waves. CFD data is compared against those collected in towing tank tests, revealing a satisfactory level of accuracy. Extreme value and gamma distributions are shown to give probabilities of maxima/minima of displacements and vertical acceleration at the center of gravity (CG) for all three hulls. It is shown that the stepless boat may be exposed to lower vertical acceleration at an early planing speed, but at higher planing speeds, a double-stepped design mitigates the vertical acceleration. Nevertheless, the double-stepped hull would experience more significant extreme heave responses across all speeds and may be exposed to less significant extreme pitch responses during the ride at the highest speed compared to the stepless and one-stepped hulls. The skewness of heave and pitch is evaluated, and it is found that the heave response tends to skew toward positive values (upward). This skewness becomes more noticeable with increasing speed but remains insensitive to wave steepness. Additionally, the pitch response at lower planing speeds shows a partial skew towards negative values (bow-down), but eventually, they may also be partially skewed towards positive values at higher speeds. Moreover, a correlation is observed between the kurtosis of responses of different hulls and the occurrence of the 1/100 highest responses, indicating that a kurtosis greater than 3.0 would result in more extreme responses. Overall, this analysis offers practical insights into planing hull behavior in actual sea conditions from a CFD model perspective, highlighting the potential of CFD in simulating this complex problem.

Experimental and numerical investigation on the hydroelastic response of barge and KVLCC2 ship

In this paper, the hydroelasticity of two segmented ship models (Barge and KVLCC2) is investigated by experimental and numerical methods. A two-way Computational Fluid Dynamics (CFD) and Finite Element Analysis (FEA) coupled method is adopted and further validated its accuracy against experimental results. The current approach emphasizes the overall convergence between two solvers, maintaining a strongly coupled manner to comprehensively address the fluid-structure interaction phenomenon, including the added mass effect. A series of experiments of a segmented Barge and KVLCC2 under various wave conditions were firstly conducted. The motions of the models are measured and the displacement Response Amplitude Operators (RAOs) are calculated. The CFD-FEA coupled method is demonstrated in agreement with experimental results by comparing the numerical prediction with the tank test results in terms of motion, affirming the validity and robustness. Finally, the hydroelastic response is discussed and investigated.

Impact of Salinity Fluctuations on Dunaliella salina Biomass Production

The utilization of microalgae as a green carbon source for chemical production has attracted attention for its potential use in sustainable and climate-friendly solutions. This study investigates the growth of Dunaliella salina, a unicellular green microalga, in response to salinity variations and water and seawater addition to compensate for evaporation in open cultures. The impact of continuous and non-continuous water addition, as well as seawater addition, on the growth of D. salina was analyzed though tank tests. The results showed that different water-addition methods did not significantly influence cell concentrations, indicating the organism’s resilience to salinity changes. Continuous water addition maintained stable salinity levels at 12%, but required continuous monitoring, while non-continuous addition reduced the intervention frequency. The overall results showed that a salinity range between 12 and 15% did not affect microalgae growth, suggesting flexibility in evaporation-loss compensation methods based on cultivation-system specifics and resource availability. Maintaining consistent biomass regardless of the water-addition method used suggests sustainable production within the tested salinity range, with seawater addition making microalgae cultivation more adaptable to regions with varying water availability. Further research, including outdoor pilot tests, is recommended to validate and extend these findings to natural environments.

Application of 2E-2U method for free-field underwater calibrations of hydrophones and projectors in a reverberant laboratory test tank

In this paper, application of a Two-Equations Two-Unknowns (2E-2U) method is described for calibration of hydrophones and projectors below 1 kHz in a laboratory test tank. At low frequencies, amplitude and phase measurements for the calibration of the hydrophones and projectors in the test tank are difficult to perform since echo-free time of the laboratory test tank is not large enough due to transducer initial transients and tank wall boundary reflections. To overcome these difficulties, the 2E-2U method is applied to received (windowed) signals obtained during calibration measurements. Thus, calibration measurements become possible at a frequency down to 250 Hz. These measurements in the test tank are performed for a hydrophone and a developed flextensional projector. First, the received sensitivities for the hydrophone are calculated and validated by comparisons with pressure calibration in a closed chamber. Good agreements are obtained between the two measurement platforms, with a maximum difference of 0.5 dB and uncertainty of 1.3 dB. Then, the transmitting voltage response (TVR) of the flextensional projector is calculated and compared with the calibration data obtained from the method defined in the relevant standards. Good agreements are obtained between the two TVR data with a maximum difference of 1.1 dB and uncertainty of 1.7 dB.

Control-inspired design and power optimization of an active mechanical motion rectifier based power takeoff for wave energy converters

Ocean waves have high energy density and are persistent and predictable. Yet, converting wave energy to a useable form remains challenging. A significant hurdle is the oscillatory nature of waves resulting in the alternating loads, which necessitate the use of rectification at some stage of the energy conversion. This research effort presents a novel design of active mechanical motion rectifier (AMMR) for the power takeoff (PTO), which provides enhanced controllability and better power performance when compared to passive mechanical motion rectifiers (MMR). Inspired by transistors used in synchronous electrical rectifiers, the proposed design uses controllable electromagnetic clutches in the mechanical transmission to allow active engagement-disengagement control; thus, rectifying the oscillatory motion into a unidirectional rotation for high energy conversion efficiency and allowing the generator in unidirectional rotation to control the bidirectional wave capture structure for maximizing the power output. A semi-analytical computational approach is developed to efficiently evaluate the optimal power achieved using the proposed AMMR-based PTO and active control. It is found that the AMMR-based PTO design yields a higher optimal power than the previous passive MMR design across the wave spectrum. The influences of generator inertia and reactive power are discussed. The effects of control parameters on the power output and the optimal trajectories are analyzed. Wave tank tests with the AMMR prototype demonstrated the effectiveness of AMMR based PTO design and validated the numerical analysis.

The Properties of Normal Concrete with Ground Manganese Slag as Binder Replacement

The Properties of Normal Concrete with Ground Manganese Slag as Binder Replacement

Aging in zebrafish is associated with reduced locomotor activity and strain dependent changes in bottom dwelling and thigmotaxis.

Aging is associated with a wide range of physiological and behavioral changes in many species. Zebrafish, like humans, rodents, and birds, exhibits gradual senescence, and thus may be a useful model organism for identifying evolutionarily conserved mechanisms related to aging. Here, we compared behavior in the novel tank test of young (6-month-old) and middle aged (12-month-old) zebrafish from two strains (TL and TU) and both sexes. We find that this modest age difference results in a reduction in locomotor activity in male fish. We also found that background strain modulated the effects of age on predator avoidance behaviors related to anxiety: older female TL fish increased bottom dwelling whereas older male TU fish decreased thigmotaxis. Although there were no consistent effects of age on either short-term (within session) or long-term (next day) habituation to the novel tank, strain affected the habituation response. TL fish tended to increase their distance from the bottom of the tank whereas TU fish had no changes in bottom distance but instead tended to increase thigmotaxis. Our findings support the use of zebrafish for the study of how age affects locomotion and how genetics interacts with age and sex to alter exploratory and emotional behaviors in response to novelty.

Approximate Dynamic Programming for Control of Wave Energy Converters With Implementation and Validation on a Point Absorber Prototype

It is recognized that the application of constrained optimal control for wave energy converters (WECs), represented by model predictive control (MPC), is hindered by its computation burden resulting from online optimization, especially when the model contains nonlinearities. In this article, a novel control solution based on approximate dynamic programming (ADP) is proposed, in which an explicit controller is solved by model sampling and neural network training. In this way, the major computation load is moved offline to enable fast online execution, and the need for wave prediction is eliminated. The effectiveness of ADP is verified by a simulation case study based on a nonlinear WEC system. Using nonlinear MPC as the performance benchmark, over 90% energy efficiency is achieved by ADP under various sea states with constraint satisfaction and dramatically faster computation. The ADP controller is further experimentally validated on a newly constructed WEC prototype through wave tank testing, where successful implementation and satisfactory performance are demonstrated.

Chronic Behavioral and Neurochemical Effects of Four Novel N-Benzyl-2-phenylethylamine Derivatives Recently Identified as "Psychoactive" in Adult Zebrafish Screens.

Potently affecting human and animal brain and behavior, hallucinogenic drugs have recently emerged as potentially promising agents in psychopharmacotherapy. Complementing laboratory rodents, the zebrafish (Danio rerio) is a powerful model organism for screening neuroactive drugs, including hallucinogens. Here, we tested four novel N-benzyl-2-phenylethylamine (NBPEA) derivatives with 2,4- and 3,4-dimethoxy substitutions in the phenethylamine moiety and the -F, -Cl, and -OCF3 substitutions in the ortho position of the phenyl ring of the N-benzyl moiety (34H-NBF, 34H-NBCl, 24H-NBOMe(F), and 34H-NBOMe(F)), assessing their behavioral and neurochemical effects following chronic 14 day treatment in adult zebrafish. While the novel tank test behavioral data indicate anxiolytic-like effects of 24H-NBOMe(F) and 34H-NBOMe(F), neurochemical analyses reveal reduced brain norepinephrine by all four drugs, and (except 34H-NBCl) - reduced dopamine and serotonin levels. We also found reduced turnover rates for all three brain monoamines but unaltered levels of their respective metabolites. Collectively, these findings further our understanding of complex central behavioral and neurochemical effects of chronically administered novel NBPEAs and highlight the potential of zebrafish as a model for preclinical screening of small psychoactive molecules.