Structure Of Terrestrial Ecosystems Research Articles

Proximal remote sensing: an essential tool for bridging the gap between high-resolution ecosystem monitoring and global ecology.

A new proliferation of optical instruments that can be attached to towers over or within ecosystems, or 'proximal' remote sensing, enables a comprehensive characterization of terrestrial ecosystem structure, function, and fluxes of energy, water, and carbon. Proximal remote sensing can bridge the gap between individual plants, site-level eddy-covariance fluxes, and airborne and spaceborne remote sensing by providing continuous data at a high-spatiotemporal resolution. Here, we review recent advances in proximal remote sensing for improving our mechanistic understanding of plant and ecosystem processes, model development, and validation of current and upcoming satellite missions. We provide current best practices for data availability and metadata for proximal remote sensing: spectral reflectance, solar-induced fluorescence, thermal infrared radiation, microwave backscatter, and LiDAR. Our paper outlines the steps necessary for making these data streams more widespread, accessible, interoperable, and information-rich, enablingus to address key ecological questions unanswerable from space-based observations alone and, ultimately, to demonstrate the feasibility of these technologies to address critical questions in local and global ecology.

Scaling of shoot and root respiration of woody and herbaceous plants.

Woody and herbaceous plants are the main components of global terrestrial ecosystems, and their growth, adaptation and survival depend largely on the metabolism of shoots and roots. Therefore, understanding size-scaling of metabolic rates in woody and herbaceous plants, and in shoots and roots, is a fundamental issue in ecology. However, few empirical studies have examined metabolic scaling exponents across a wide range of plant sizes. Using whole-plant chamber systems, we measured respiration rates of entire root systems and shoots of 96 woody species (n = 1243) and 33 herbaceous species (n = 463) from various terrestrial biomes, with plant masses spanning nine orders of magnitude. Scaling exponents for relationships between respiration rates and fresh mass were greater in shoots than in roots, and both were greater in herbaceous plants than in woody plants. Furthermore, scaling of whole-plant respiration, including various species, converged separately for woody and herbaceous plants. These findings suggest some general physico-chemical constraints on energy use by shoots and roots of individual plants in various terrestrial biomes, including forests and grasslands. These data will advance our understanding of terrestrial ecosystem structure and function.

Effects of Extreme Climatic Events on the Autumn Phenology in Northern China Are Related to Vegetation Types and Background Climates

The increased intensity and frequency of extreme climate events (ECEs) have significantly impacted vegetation phenology, further profoundly affecting the structure and functioning of terrestrial ecosystems. However, the mechanisms by which ECEs affect the end of the growing season (EOS), a crucial phenological phase, remain unclear. In this study, we first evaluated the temporal variations in the EOS anomalies in Northern China (NC) based on the Normalized Difference Vegetation Index (NDVI) and Enhanced Vegetation Index (EVI) from 2001 to 2018. We then used event coincidence analysis (ECA) to assess the susceptibility of EOS to four ECEs (i.e., extreme heat, extreme cold, extreme wet and extreme dry events). Finally, we examined the dependence of the response of EOS to ECEs on background climate conditions. Our results indicated a slight decrease in the proportion of areas experiencing extreme heat and dry events (1.10% and 0.66% per year, respectively) and a slight increase in the proportion of areas experiencing extreme wet events (0.77% per year) during the preseason period. Additionally, EOS exhibited a delaying trend at a rate of 0.25 days/a during the study period. The susceptibility of EOS to ECEs was closely related to local hydrothermal conditions, with higher susceptibility to extreme dry and extreme hot events in drier and warmer areas and higher susceptibility to extreme cold and extreme wet events in wetter regions. Grasslands, in contrast to forests, were more sensitive to extreme dry, hot and cold events due to their weaker resistance to water deficits and cold stress. This study sheds light on how phenology responds to ECEs across various ecosystems and hydrothermal conditions. Our results could also provide a valuable guide for ecosystem management in arid regions.

Stronger Impact of Extreme Heat Event on Vegetation Temperature Sensitivity under Future Scenarios with High-Emission Intensity

Vegetation temperature sensitivity is a key indicator to understand the response of vegetation to temperature changes and predict potential shifts in ecosystem functions. However, under the context of global warming, the impact of future extreme heat events on vegetation temperature sensitivity remains poorly understood. Such research is crucial for predicting the dynamic changes in terrestrial ecosystem structure and function. To address this issue, we utilized historical (1850–2014) and future (2015–2100) simulation data derived from CMIP6 models to explore the spatiotemporal dynamics of vegetation temperature sensitivity under different carbon emission scenarios. Moreover, we employed correlation analysis to assess the impact of extreme heat events on vegetation temperature sensitivity. The results indicate that vegetation temperature sensitivity exhibited a declining trend in the historical period but yielded an increasing trend under the SSP245 and SSP585 scenarios. The increasing trend under the SSP245 scenario was less pronounced than that under the SSP585 scenario. By contrast, vegetation temperature sensitivity exhibited an upward trend until 2080 and it began to decline after 2080 under the SSP126 scenario. For all the three future scenarios, the regions with high vegetation temperature sensitivity were predominantly located in high latitudes of the Northern Hemisphere, the Tibetan Plateau, and tropical forests. In addition, the impact of extreme heat events on vegetation temperature sensitivity was intensified with increasing carbon emission intensity, particularly in the boreal forests and Siberian permafrost. These findings provide important insights and offer a theoretical basis and guidance to identify climatically sensitive areas under global climate change.

Urbanization alters sandy beach scavenging assemblages and dogs suppress ecosystem function

AbstractUrbanization is rapidly transforming coastal landscapes around the world, altering the structure and function of marine, intertidal, and terrestrial ecosystems. In tandem, coastal areas are hotspots for human recreation, leading to shifts in wildlife behavior and activity patterns. Together, urban development and recreational use of wildlife habitats can shape wildlife behavior, abundance, and ecosystem dynamics at different spatial and temporal scales. In this study, we explore the impact of urbanization and human and domestic dog activity on the structure of vertebrate scavenging assemblages and the ecosystem functions they provide in sandy beach ecosystems across 40 km of the central California coast, USA. We surveyed vertebrate scavenging assemblages using baited camera traps on 17 beaches spanning a gradient of coastal urbanization. We found that urbanization extent within small spatial scales (i.e., 1 or 3 km radii of each site) and the rate of beach visitation by domestic dogs or humans were the best additive predictors of assemblage structure. We identified pronounced urbanization‐associated shifts in the composition of vertebrate scavenger guilds but found that the rate of carrion processing was more strongly influenced by domestic dog habitat use and diel period. Scavenging activity was substantially lower on beaches with more domestic dogs, suggesting that dogs interfere with critical scavenging ecosystem functions on sandy beaches. Our results underscore the pervasive and nuanced effects of urbanization and recreation on the dynamics of land–sea connectivity and suggest a need for comprehensive consideration of cross‐ecosystem linkages in ongoing shoreline conservation and development efforts.

Biodiversity conservation and ecological restoration dominated vegetation dynamics during the 1980s-2010s in Yunnan, China

Changes in both vegetation type and productivity can affect the structure, functioning, and services of terrestrial ecosystems. Understanding vegetation dynamics and their drivers is critical for biodiversity conservation and ecosystem management. Although land cover datasets, climate-vegetation models, and remote sensing vegetation indices have been frequently used to reflect vegetation dynamics, they generally lack biological information about vegetation, such as species compositions, community structures, and succession status. Yunnan, recognized as the most biodiverse province in China, has undergone considerable vegetation changes over recent decades. However, the roles of climate change and human activities remain unclear. This study integrated detailed vegetation maps, climate factors, and vegetation indices obtained in the 1980s (1986–1995) and 2010s (2006–2015) to comprehensively evaluate the coverage transformations and productivity changes of vegetation types in Yunnan and unravel the drivers of decadal vegetation changes. The results indicated that: 1) A greening trend was observed across all vegetation types, particularly in coniferous and temperate forests. 2) The decadal vegetation changes were dominated by: the restoration of savanna and shrubland, cropland expansion, and artificial afforestation, accounting for 23.7 %, 22.9 %, and 19.1 % of all observed changes, respectively. 3) Conservation and restoration efforts dominated vegetation greening in Yunnan (55 %), followed by artificial afforestation (23 %), and agricultural expansion (16 %). By comparing vegetation maps of separated stages, the inclusion of vegetation class information provided critical knowledge for a better understanding of the intrinsic mechanisms behind vegetation greening and range shifting. Our study highlighted the significant role of ecological conservation and restoration policies and practices in influencing the spatiotemporal dynamics of biodiversity and ecosystem functioning at a regional scale, within merely a few decades.

Multi-Scenario Simulation of Land-Use/Land-Cover Changes and Carbon Storage Prediction Coupled with the SD-PLUS-InVEST Model: A Case Study of the Tuojiang River Basin, China

Land-use and land-cover changes (LUCCs) significantly impact carbon sequestration by modifying the structure and function of terrestrial ecosystems. This study utilized GIS and remote sensing techniques to forecast future LUCC patterns and their influence on regional carbon budgets, which is essential for sustainable development. We devised a coupled system dynamics (SD) model integrated with a patch-generating land-use simulation (PLUS) model to simulate LUCCs under diverse future scenarios using multisource environmental data. Additionally, the InVEST model was employed to quantify carbon storage in terrestrial ecosystems. By establishing three scenarios—ecological priority (EP), highly urbanized (HU), and coordinated development (CD)—this study’s aim was to predict the LUCC patterns and carbon storage distribution of the Tuojiang River Basin (TRB), China, up to 2035. The results showed that (1) from 2000 to 2020, significant LUCCs occurred in the TRB, primarily involving the conversion of cultivated land into construction areas and forestland; (2) LUCCs had a substantial impact on carbon storage in the TRB, with the EP scenario demonstrating the highest carbon storage by 2035 due to extensive forest expansion, while the HU scenario indicated a decline in carbon storage associated with rapid urbanization; and (3) the mountainous regions of the TRB, dominated by forestland, consistently exhibited higher carbon storage, whereas the Chengdu Plain region in the upper basin displayed the lowest. In conclusion, we recommend prioritizing the CD scenario in future development strategies to balance economic growth with ecological protection while simultaneously enhancing carbon storage. Our findings offer valuable insights to shape future LUCC policies in the Tuojiang River Basin, underscoring the adaptability of the coupled model approach to a wide range of geographic scales and contexts.

Plant-insect interactions in the mid-Cretaceous paleotropical El Chango Lagerstätte (Cintalapa Fm., Mexico)—patterns of herbivory during the Angiosperm Terrestrial Revolution

Plants and insects are two of the more diverse and abundant organisms in terrestrial ecosystems. The fossil record of plant-insect interactions offers crucial insights into the coevolutionary dynamics between these groups, shedding light on the intricate relationships that have shaped terrestrial ecosystems. The study of fossil interactions is especially relevant in mid-Cretaceous ecosystems, a time of dramatic changes in the composition of floras and, consequently, in plant-insect relationships. Here, we describe the first suite of plant-insect interactions from the mid-Cretaceous of Mexico. We studied 554 plant fossils from the El Chango Lagerstätte (Cintalapa Formation, Chiapas, Mexico), including vegetative (leaves) and reproductive structures (fruits and seeds). The flora was dominated by gymnosperms (89.3%) followed by angiosperms (10.7%); other groups, such as pteridophytes and bryophytes, were absent. In total, 5.4% of the plant specimens hosted some damage. Angiosperms (all broad-leafed forms), despite being much less common than gymnosperms, expressed more evidence of damage by herbivores (35.6% of specimens damaged). In contrast, the narrow-leafed gymnosperms, the dominant group in the flora, hosted a much lower proportion of herbivory damage (1.8% of specimens damaged). The diversity of damage types (DTs) was relatively low: 14 DTs were identified, corresponding to seven FFGs, including margin feeding, hole feeding, surface feeding, piercing and sucking, oviposition, galling, and mining. Comparison with the other mid-Cretaceous plant-insect assemblages reveals a similar richness of DTs for angiosperms but a lower richness and diversity of DTs on gymnosperms from El Chango. These results indicate preferential herbivory on angiosperms (rather than on the available gymnosperms in the assemblage) by terrestrial arthropods during a period of major changes in the structure of terrestrial ecosystems. However, it is challenging to resolve whether this apparent preference is because insects particularly targeted angiosperms or if the herbivores simply targeted broad leaves in general, since most of the available gymnosperms from El Chango are scale-leafed forms.

A global dataset of the fraction of absorbed photosynthetically active radiation for 1982–2022

The fraction of absorbed photosynthetically active radiation (FPAR) is an essential biophysical parameter that characterizes the structure and function of terrestrial ecosystems. Despite the extensive utilization of several satellite-derived FPAR products, notable temporal inconsistencies within each product have been underscored. Here, the new generation of the GIMMS FPAR product, GIMMS FPAR4g, was developed using a combination of a machine learning algorithm and a pixel-wise multi-sensor records integration approach. PKU GIMMS NDVI, which eliminates the orbital drift and sensor degradation issues, was used as the data source. Comparisons with ground-based measurements indicate root mean square errors ranging from 0.10 to 0.14 with R-squared ranging from 0.73 to 0.87. More importantly, our product demonstrates remarkable spatiotemporal coherence and continuity, revealing a persistent terrestrial darkening over the past four decades (0.0004 yr−1, p < 0.001). The GIMMS FPAR4g, available for half-month intervals at a spatial resolution of 1/12° from 1982 to 2022, promises to be a valuable asset for in-depth analyses of vegetation structures and functions spanning the last 40 years.

Nitrogen addition has divergent effects on phosphorus fractions in four types of soils

BackgroundGlobally increasing atmospheric nitrogen (N) deposition has altered soil phosphorus (P) transformations and availability, and thereby influenced structure and function of terrestrial ecosystems. Edaphic characteristics and chemical form of deposited N could be important factors determining impacts of N deposition on soil P transformations, yet the underlying mechanisms remain largely unknown. Objectives of this study were to examine how mineral-N and amino N differently affect P fractions, and identify key soil properties determining N addition impacts on soil P transformations. Considering that amino N is an important component of deposited N and forest soils vary greatly in different regions, the results of present study can guide the management of forests across different soils under ongoing N deposition scenarios.MethodsWe conducted a 60-day laboratory experiment to investigate the effects of N addition (NH4NO3 and glycine) on soil P fractions and related biochemical properties in four representative forest soils (brown, yellow brown, aeolian sandy, and red soils) in China. Glycine and NH4NO3 were separately added at three rates (5, 10 and 20 g N m–2 yr–1).ResultsFirstly, the percent changes in organic P fractions with N addition were significantly greater than changes in inorganic P fractions across all soils. Secondly, the percent changes in P fractions with glycine and NH4NO3 additions were significantly correlated across all soils and treatments. However, glycine addition had significantly greater impacts on organic P fractions than NH4NO3 addition in the aeolian sandy and red soils with low organic carbon content. Thirdly, P fractions responded differently to N addition among the four soils. N-induced changes in microbial biomass and phosphatase activities, pH, exchangeable Ca2+ and Mg2+ contributed differently to the changes in P fractions with N addition in the four soils.ConclusionsThe different responses of P fractions to N addition in the four soils were mainly generated by the differences in extent of microbial N limitation, acid buffering capacity, and cation exchange capacity among the soils. The different impacts of mineral and amino N on soil P fractions can be ascribed to their divergent effects on soil pH, microbial biomass and activities.

Elevation-dependent pattern of net CO2 uptake across China

The elevation gradient has long been known to be vital in shaping the structure and function of terrestrial ecosystems, but little is known about the elevation-dependent pattern of net CO2 uptake, denoted by net ecosystem productivity (NEP). Here, by analyzing data from 203 eddy covariance sites across China, we report a negative linear elevation-dependent pattern of NEP, collectively shaped by varying hydrothermal factors, nutrient supply, and ecosystem types. Furthermore, the NEP shows a higher temperature sensitivity in high-elevation environments (3000–5000 m) compared with the lower-elevation environments (<3000 m). Model ensemble and satellite-based observations consistently reveal more rapid relative changes in NEP in high-elevation environments during the last four decades. Machine learning also predicts a stronger relative increase in high-elevation environments, whereas less change is expected at lower elevations. We therefore conclude a varying elevation-dependent pattern of the NEP of terrestrial ecosystems in China, although there is significant uncertainty involved.

Global change progressively increases foliar nitrogen-phosphorus ratios in China's subtropical forests.

Globally increased nitrogen (N) to phosphorus (P) ratios (N/P) affect the structure and functioning of terrestrial ecosystems, but few studies have addressed the variation of foliar N/P over time in subtropical forests. Foliar N/P indicates N versus P limitation in terrestrial ecosystems. Quantifying long-term dynamics of foliar N/P and their potential drivers is crucial for predicting nutrient status and functioning in forest ecosystems under global change. We detected temporal trends of foliar N/P, quantitatively estimated their potential drivers and their interaction between plant types (evergreen vs. deciduous and trees vs. shrubs), using 1811 herbarium specimens of 12 widely distributed species collected during 1920-2010 across China's subtropical forests. We found significant decreases in foliar P concentrations (23.1%) and increases in foliar N/P (21.2%). Foliar N/P increased more in evergreen species (22.9%) than in deciduous species (16.9%). Changes in atmospheric CO2 concentrations ( ), atmospheric N deposition and mean annual temperature (MAT) dominantly contributed to the increased foliar N/P of evergreen species, while , MAT, and vapor pressure deficit, to that of deciduous species. Under future Shared Socioeconomic Pathway (SSP) scenarios, increasing MAT and would continuously increase more foliar N/P in deciduous species than in evergreen species, with more 12.9%, 17.7%, and 19.4% versus 6.1%, 7.9%, and 8.9% of magnitudes under the scenarios of SSP1-2.6, SSP3-7.0, and SSP5-8.5, respectively. The results suggest that global change has intensified and will progressively aggravate N-P imbalance, further altering community composition and ecosystem functioning of subtropical forests.

Gene drives, mosquitoes, and ecosystems: an interdisciplinary approach to emerging ethical concerns

Gene drives are genetic elements that in sexually reproducing organisms spread faster than those transmitted through a Mendelian fashion. Since gene drives can be engineered to modify different aspects of physiology and reproduction, they have been proposed as a new and revolutionary tool to control vector-borne diseases, particularly those transmitted by the genera Anopheles and Aedes (Culicidae), such as malaria, Dengue and Zika virus. This approach may impact on human health by lowering the transmission of such devastating diseases. However, the release of genetically modified mosquitos (or other species) into the environment raises a series of questions related to the still incipient technology and our present understanding of the complex structure and dynamics of terrestrial and aquatic ecosystems. Moreover, there are ethical concerns about human interventions in natural ecosystems that may eventually impact our way of living or the ecosystems themselves. This work is an interdisciplinary approach that analyzes from a biological, philosophical, and theological perspective the potential ecological impacts on natural environments of the release of genetically modified species, focusing on gene drive-modified mosquitos. It includes theological approach from a Catholic point of view (although it could be easily shared by other Christians) because we hold that world religions give valuable insights even though not everyone may share their groundings. We conclude that the focal problem is the relationship between humans and nature, and the release of genetically modified species may change this relationship unpredictably. However, given the complex interactions in ecosystems, new approaches such as Earth Stewardship principles could provide new and more widely accepted answers involving biological, philosophical, and theological concepts that will help engaging all relevant actors to make a better world.

Validating a multi-locus metabarcoding approach for characterizing mixed-pollen samples

BackgroundThe mutualistic interaction between entomophilous plants and pollinators is fundamental to the structure of most terrestrial ecosystems. The sensitive nature of this relationship has been disrupted by anthropogenic modifications to natural landscapes, warranting development of new methods for exploring this trophic interaction. Characterizing the composition of pollen collected by pollinators, e.g. Apis mellifera, is a common means of exploring this relationship, but traditional methods of microscopic pollen assessment are laborious and limited in their scope. The development of pollen metabarcoding as a method of rapidly characterizing the abundance and diversity of pollen within mixed samples presents a new frontier for this type of work, but metabarcoding may have limitations, and validation is warranted before any suite of primers can be confidently used in a research program. We set out to evaluate the utility of an integrative approach, using a set of established primers (ITS2 and rbcL) versus melissopalynological analysis for characterizing 27 mixed-pollen samples from agricultural sites across Canada.ResultsBoth individual markers performed well relative to melissopalynology at the family level with decreases in the strength of correlation and linear model fits at the genus level. Integrating data from both markers together via a multi-locus approach provided the best rank-based correlation between metagenetic and melissopalynological data at both the genus (ρ = 0.659; p < 0.001) and family level (ρ = 0.830; p < 0.001). Species accumulation curves indicated that, after controlling for sampling effort, melissopalynological characterization provides similar or higher species richness estimates than either marker. The higher number of plant species discovered via the metabarcoding approach simply reflects the vastly greater sampling effort in comparison to melissopalynology.ConclusionsPollen metabarcoding performed well at characterizing the composition of mixed pollen samples relative to a traditional melissopalynological approach. Limitations to the quantitative application of this method can be addressed by adopting a multi-locus approach that integrates information from multiple markers.

Tracing climatic and human disturbance in diverse vegetation zones in China: Over 20 years of NDVI observations

Over the past two decades (2000–2021), China has experienced a significant increase in vegetation cover. However, the intricate spatial and temporal variations in vegetation cover, arising from the interaction of climate change and human activities, remain enigmatic, particularly across diverse vegetation zones. This research endeavors to unravel the dynamics of vegetation cover using the MOD13A1 Normalized Difference Vegetation Index (NDVI) dataset, delving into China's seven distinct vegetation zones (VZ1-VZ7). This research employs residual analysis, relative analysis, and geo-detector techniques to investigate the drivers of NDVI variation and spatial heterogeneity across China's seven distinct vegetation zones. The findings reveal a compelling upward trajectory in NDVI, predicted NDVI, and residual NDVI across China during the 2000–2021 period. Notably, significant and extremely significant increases are discernible in approximately 53.90%, 50.13%, and 23.29% of China's NDVI, respectively. Among the vegetation zones, VZ3 displays the highest change rates, yet the proportion with a significant increase in VZ1 surpasses other zones. The relative analysis underscores climate change as the predominant catalyst for vegetation enhancement, although an exception is noted in VZ3. Furthermore, the spatial heterogeneity of China's NDVI is primarily influenced by the interactions between land use types and relative humidity, sunshine duration, and precipitation, especially the interaction between relative humidity and land use types. Significant changes in climate can lead to marked alterations in the structure and function of terrestrial ecosystems, while changes in land use patterns at the regional level also alter the type of land cover, which together exert a significant impact on the vegetation NDVI. These results accentuate the importance of formulating tailored ecosystem management strategies tailored to the distinct characteristics of each vegetation zone to ensure the sustainable development of terrestrial ecosystems across China.

Water rather than nitrogen availability predominantly modulates soil microbial beta-diversity and co-occurrence networks in a secondary forest

Atmospheric nitrogen (N) deposition and changing precipitation regimes greatly affect the structure and functions of terrestrial ecosystems. However, their impacts on the diversity and assembly of soil microbial communities including bacteria, fungi and protists, remain largely unclear. As part of a six-year field experiment in a secondary forest in a warm temperate and subtropical climate transitional zone in China, we aimed to investigate the responses of soil microbial communities to N addition, increased and decreased precipitation. The results showed that N addition had no effect on soil microbial α- or β-diversity, but reduced the complexity of microbial network. Neither increased nor decreased precipitation influenced soil microbial α-diversity, but decreased precipitation rather than increased precipitation elevated bacterial and protistan community dissimilarities (β-diversity), which could have been largely attributed to species replacement processes through reducing soil water availability. In addition, decreased precipitation weakened microbial complexity and stability, but enhanced the node proportion of protists in the co-occurrence network. Our observations suggest the asymmetric responses of soil microbial β-diversity to increased and decreased precipitation, and underscore that water rather than N availability, especially drought condition, plays a predominant role in modulating soil microbial β-diversity. Moreover, the findings imply that global change can strengthen the importance of soil protists and then reshape microbial assembly in forests.

C:N:P stoichiometry of plants, soils, and microorganisms: Response to altered precipitation.

Precipitation changes modify C, N, and P cycles, which regulate the functions and structure of terrestrial ecosystems. Although altered precipitation affects above- and belowground C:N:P stoichiometry, considerable uncertainties remain regarding plant-microbial nutrient allocation strategies under increased (IPPT) and decreased (DPPT) precipitation. We meta-analyzed 827 observations from 235 field studies to investigate the effects of IPPT and DPPT on the C:N:P stoichiometry of plants, soils, and microorganisms. DPPT reduced leaf C:N ratio, but increased the leaf and root N:P ratios reflecting stronger decrease of P compared with N mobility in soil under drought. IPPT increased microbial biomass C (+13%), N (+15%), P (26%), and the C:N ratio, whereas DPPT decreased microbial biomass N (-12%) and the N:P ratio. The C:N and N:P ratios of plant leaves were more sensitive to medium DPPT than to IPPT because drought increased plant N content, particularly in humid areas. The responses of plant and soil C:N:P stoichiometry to altered precipitation did not fit the double asymmetry model with a positive asymmetry under IPPT and a negative asymmetry under extreme DPPT. Soil microorganisms were more sensitive to IPPT than to DPPT, but they were more sensitive to extreme DPPT than extreme IPPT, consistent with the double asymmetry model. Soil microorganisms maintained stoichiometric homeostasis, whereas N:P ratios of plants follow that of the soils under altered precipitation. In conclusion, specific N allocation strategies of plants and microbial communities as well as N and P availability in soil critically mediate C:N:P stoichiometry by altered precipitation that need to be considered by prediction of ecosystem functions and C cycling under future climate change scenarios.

Phosphorus Coupled with High Nitrogen Addition Exerts a Great Influence on Soil Bacterial Community in a Semiarid Grassland.

Nitrogen (N) and phosphorus (P) addition, either individually or in combination, has been demonstrated to enhance plant productivity in grassland ecosystems. Soil bacterial community, which is the driver of litter decomposition and nutrient cycling, is assumed to control responses of terrestrial ecosystem structure and function to N and P addition. Using a high-throughput Illumina MiSeq sequencing platform, we conducted a 9-year field experiment of N (0, 5, 10, and 20 g N m-2 yr-1) and P (0 and 10 g P m-2 yr-1) additions in the Inner Mongolian steppes to elucidate long-term effects of N and P addition on soil bacterial richness, diversity and composition. We found that N addition reduced the relative abundance of Acidobacteria, Chloroflexi, and Nitrospirae, while increased that of Bacteroides. The results showed that the bacterial biomarker was enriched in P addition treatments, either individually or combined with N addition. Both N and P addition altered the bacterial community structure, while only N addition greatly decreased bacterial richness and diversity. More importantly, we showed that all of these effects were most significant in N3P treatment (20 g N m-2 yr-1 and 10 g P m-2 yr-1), implying that P coupled with a high-level N addition exerted a great influence on soil bacterial community. Structural equation models revealed that N and P addition had a great direct effect on soil bacterial community and an indirect effect on it mainly by changing the litter biomass. Our findings highlighted that severe niche differentiation was induced by P along with a high-level N, further emphasizing the importance of simultaneously evaluating response of soil bacterial community to N and P addition, especially in the context of increasing anthropogenic nutrient additions.

Projected Rainfall‐Driven Expansion of Woody Cover in African Drylands

AbstractProjection of future woody cover is essential to understand potential changes in structure and functioning of terrestrial ecosystems. Previous studies mapped woody cover during historical periods observed by satellites, however, it remains unclear how woody cover is expected to change in response to future climate change. Here, we develop data‐driven models to predict woody cover in Africa using multiple environmental predictors and show that woody cover can be accurately modeled using Random Forest. Empirically‐based simulations forced by precipitation from CMIP6 project an overall increase in woody cover at the continental scale by 2100. However, increases are mainly occurring in regions with annual precipitation less than ∼1,600 mm y−1, whereas woody cover is predicted to decrease in areas of higher rainfall. Our results suggest that climate change may alter the functioning of dryland ecosystems by continued woody encroachment and cause a loss of carbon stocks in humid areas.

Impact of litter burning on alpine &lt;i&gt;Festuca varia&lt;/i&gt; grasslands of the Northwestern Caucasus

Fires play an important role in structure and function of terrestrial ecosystems, but their long-term impact on the composition and structure of plant communities in humid high mountain regions remains almost not studied. At the most dry alpine grasslands, dominated by a dense-tussock grass Festuca varia, with substantial accumulation of non-decomposed litter, the 23-years long experiment with regular (every two years) litter burning was established. The composition of plant community changed significantly. The mortmass (mass of litter), aboveground vascular plant biomass and relative abundance of dominants decreased substantially. In aboveground biomass the proportion of grasses decreased and that of forbs increased. The shoot numbers of Anthemis cretica, Campanula collina, Deschampsia flexuosa, Festuca ovina, Nardus stricta, and Veronica gentianoides increased after burning. Two-fold increase of alpha-diversity of vascular plants was observed on plots with burning treatment, it was twice as high as initial value, and was significantly higher than the values in the control plots. Long-term burning did not substantially change mean P, Ca and Mg content in the biomass of the most of studied species, only K content decreased in some species, while Mg content increased in Festuca varia and Nardus stricta. The increase of P and Mg content in the mortmass was observed. During long-term burning, weak soil acidification and the decrease of Ca content, as well as strong decrease of nitrogen content and the intensity of nitrogen transformation processes were observed. Generally, the observed patterns were similar to those in other studied herb communities, however, the decrease of K content during the regular burning was not reported earlier.