Terms Of Potential Research Articles

The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis.

Periodontitis (PD) is a degenerative, bacteria-induced chronic disease of periodontium causing bone resorption and teeth loss. It includes a strong reaction of immune cells through the secretion of proinflammatory factors such as Interleukin-17 (IL-17). PD treatment may consider systemic oral antibiotics application, including doxycycline (Dox), exhibiting antibacterial and anti-inflammatory properties along with supportive activity in wound healing, thus affecting alveolar bone metabolism. In the present study, we aimed to determine whether Dox can affect the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) modulated by IL-17 in terms of cell migration, osteogenic potential, bioenergetics and expression of extracellular matrix metalloproteinase 2 (MMP-2). Our findings indicate that Dox reduces the stimulatory effect of IL-17 on migration and MMP-2 expression in PDLSCs. Furthermore, Dox stimulates osteogenic differentiation of PDLSCs, annulling the inhibitory effect of IL-17 on PDLSCs osteogenesis. In addition, analyses of mitochondrial respiration reveal that Dox decreases oxygen consumption rate in PDLSCs exposed to IL-17, suggesting that changes in metabolic performance can be involved in Dox-mediated effects on PDLSCs. The pro-regenerative properties of Dox in inflammatory microenvironment candidates Dox in terms of regenerative therapy of PD-affected periodontium are observed.

The impact of lithiated active binder on Li-ion battery charge–discharge and rate capability, Part II: Mesoscale modeling of LCO based Half-cell

Even though thick electrodes are essential to increasing the energy density of LIBs, high current rates cause them to lose power density due to high polarization and ion depletion in the pore spaces. Mixed active binders with lithiated bound charges can improve the battery capacity and voltage at high C-rates, even compared to similar high concentrated electrolyte. The lithiated binders also provide a more uniform spatial distribution of intercalation reaction current, electric potential, and open circuit potential. The LIB half cell is modeled at mesoscale by taking into account the diffusion–reaction physics of the bound charge mixed electrolyte in active carbon-binder-domain (CBD), that accounts the dissociation of anionic ligands. Electrolyte in separator and active CBD are coupled by Donnan theory at the interface, and the reaction interfaces are modeled by the standard Butler–Volmer equation. By means of a thermodynamically consistent model and its implementation in a FEM code, we investigate the impact of particle distribution, electrode thickness, and separator thickness for both standard binder and active binder mixed cathodes in terms of ion depletion, current density, and electric potential. This mathematical model can also be extended to the modeling of ion conducting polymer electrolytes, gel polymers, and other conductive materials for battery applications.

Strategi Pengembangan Program BIPA di Politeknik Negeri Bali

The existence of the BIPA program at the Bali State Polytechnic is currently not developing optimally. In fact, in terms of feasibility, potential, position, and human resources, PNB has the opportunity to become a BIPA study centre in South Bali. This existing potential has not been managed and developed to support the internationalization of the Bali State Polytechnic as a vocational education that has a mission to be leading, professional, and able to compete internationally. This study was conducted to try to investigate and explore the problems that exist in the development of BIPA and try to find solutions, strategies, and actions that should be taken to raise the spirit of learning BIPA at the Bali State Polytechnic. The methods used in data collection are observation methods, interviews, and questionnaires with qualitative descriptive, SWOT, SOAR, and QSPM analysis as the analysis methods. This research resulted in (1) PNB needs to develop BIPA into a professional business unit with a culture-based; (2) Marketing mix strategies that need to be carried out to increase the diversification of BIPA learners today are integrated marketing with the use of social media, such as influencers and copywriting; (3) The strategy model and types of publications that can be done to attract BIPA learners to the Bali State Polytechnic are by collaborating with institutions between foreign universities both through research and teaching by utilizing digital publications such as facebook, instagram, tiktok, and others.

Assessment of export potential and state of foreign food and agriculture trade in the world

Purpose. The purpose of the article is to assess the export potential of countries, to determine the directions of food export development and the place of Ukraine in it, taking into account socio-economic factors by applying the authors’ methodical approach.
 Methodology / approach. The article considers world food and agriculture exports, the export potential of countries is assessed taking into account socio-economic factors. The authors select and apply a combination of methods necessary to achieve the goal of the study, which characterize specificities of individual countries of the world: method of systematic generalization, dynamic series analysis, factor analysis, regression analysis, comparisons, rating evaluation, integral approach. 
 Results. With the help of factor and integral analysis of macroeconomic indicators, we established that the structure of world exports and the share of food and agriculture is depended on the level of development and specialization of countries. In particular, the share of food and agriculture exports of developed countries is smaller compared to developing countries. The Netherlands and Germany are the leaders of the ranking in terms of food and agriculture export potential. According to forecasts, based on current trends, the export of bakery products in Ukraine and Poland should increase in 2023 compared to 2020 by 32.7 and 21.6 %, respectively. Effective socio-economic development, balanced export policy, modernization of production and logistics will contribute to the growth of export of food and agriculture with high added value.
 Originality / scientific novelty. Based on the authors’ methodical approach, the work improved the integral indicator of export development and the ranking of countries by export potential, taking into account socio-economic conditions. Factor models were built and the factors of food and agriculture export development were determined.
 Practical value / implications. The improved methodical approach to evaluating the effectiveness of global food and agriculture exports provides an opportunity to establish competitive advantages in the market and plan exports. Developing countries will have the opportunity to make the right choice of export products, which will provide them with added value and an increase in supply volumes.

An Adaptive Shift Schedule Design Method for Multi-Gear AMT Electric Vehicles Based on Dynamic Programming and Fuzzy Logical Control

This paper proposes an adaptive shift schedule design framework based on dynamic programming (DP) algorithm and fuzzy logical control to promote the shift schedule’s adaptability whilst improving the comprehensive performance of the multi-gear automated manual transmission (AMT) electric vehicles in real-time application. First, the DP algorithm is employed to extract an offline optimal gear-shift schedule based on a set of driving conditions, including 11 groups of typical driving cycles. Second, a fuzzy logical controller is formulated considering the variation in the vehicle load and acceleration, where a velocity increment is exported online to adjust the gear-shift velocity of the predesigned DP-based schedule to develop a Fuzzy-DP shift schedule. In addition, multi-objective particle swarm optimization (MOPSO) is utilized to construct a comprehensive shift schedule by simultaneously considering the dynamic and economic performance of the vehicle. Then, the dynamic and economic shift schedules are deployed as the benchmark to examine the performance of the proposed shift schedule. Finally, the effectiveness of the Fuzzy-DP shift schedule is evaluated by comparison with others under various combined driving cycles (including vehicle load and velocity). The comparisons demonstrate the remarkable promotion in the adaptability of the Fuzzy-DP shift schedule in terms of acceleration time, energy-saving potential, and shift frequency. The most significant improvements in the dynamic, economic, and shift frequency can reach 8.86%, 10.12%, and 8.56%, respectively, in contrast to the MOPSO-based shift schedule.

Proof of Concept: A New Solution for Low-Profile Transcatheter Implantation of Optimus-L Stents in Small Children

BackgroundThere is no stent designed or approved for use in infants. We sought to obtain in vitro and in vivo data on a new concept conceived to implant Optimus-L stents at infant vessel diameters and offer a potential long term stent solution. MethodsNineteen Optimus-L stents were mounted on 8 types of angioplasty balloons with diameters 6, 8, and 10 mm with the use of an injection-moulded hand crimper. We evaluated balloon-stent unit (BSU) stability before insertion and advancement through short Terumo introducers with incremental French size and possibility of side-arm contrast injections. Three types of long sheaths were tested. Stents were inflated to balloon nominal diameters and re-expanded to 18 and 23 mm. Stent recoil, foreshortening, and fracture were evaluated. In vivo implantations were performed afterward. ResultsIn vitro: Medtronic Evercross balloons and modified Terumo Destination sheaths were the best combination: BSUs were inserted in 6 F sheaths with possible injections (for 6 and 8 mm balloons), and 7 F sheaths without injections (for the 10 mm balloon). Retrieving BSUs inside the sheath required 1 additional F-size. Boston Scientific Sterling and Balton Lovix balloons, as well as APT Braidin L guiding sheaths showed unsatisfactory performance. Dilation up to 23 mm was possible, and stent shortening was < 24% at 18 mm and < 37% at 23 mm. Recoil was limited, and no stent fractured. In vivo: Optimus-L stents were used to treat 2 infants with aortic coarctation and 2 children with pulmonary artery stenosis with the use of 8 mm balloons and low-profile access. ConclusionsOptimus-L stents can be implanted safely in small patients with a low-profile approach. These stents have the potential to achieve adult size while maintaining structural integrity.

Evaluation of public submissions to the USDA for labeling of cell-cultured meat in the United States.

With the rapid advancement of cell-cultured meat processing technologies and regulations, commercialization of cell-cultured meat to market shelves requires the implementation of labeling that informs and protects consumers while ensuring economic competitiveness. In November 2022, the United States Food and Drug Administration (FDA) completed its first pre-market consultation of cell-cultured meat and did not question the safety of these products for human consumption. As of June 2023, commercialization of cell-cultured meat products has become a reality in the United States. To derive potential label terms and gain insight into how different stakeholders refer to these novel products, we analyzed 1,151 comments submitted to the 2021 U.S. Department of Agriculture's Food Safety and Inspection Services (USDA-FSIS) call on the labeling of cell-cultured meat and poultry. Our first aim was to systematically assess the nature of comments with regards to their length, cited references, and supplemental materials. In addition, we aimed to identify the most used terms to refer to these products through text analysis. We also asked how these analyses would vary by affiliation category and economic interest. Using the listed organizations for each comment, we first determined financial ties: 77 (7%) comments came from those with an economic interest, 12 (1%) of the comments did not have an identifiable economic interest, while for the remaining 1,062 (92%) comments economic interest could not be determined. We then grouped comments into affiliation categories. Cell-cultured meat companies and animal welfare non-profits had the highest median word count, whereas comments from the unknown affiliation category had the lowest. We found across all comments the predominantly mentioned potential label terms, in descending order, to be cultured meat, lab-grown meat, cultivated meat, cell-cultured meat, clean meat, and cell-based meat. While all label terms were discussed throughout overall submissions, percentages of comments mentioning each term differed between affiliation categories. Our findings suggest differences in how affiliation categories are discussing cell-cultured meat products for the US market. As a next step, the perception and acceptance of these terms must be evaluated to identify the optimal label term regarding the information and protection provided to consumers while ensuring economic competitiveness.

A comparison of FEM results from the use of different governing equations in a galvanic cell part II: Impact of low supporting electrolyte concentration

Finite Element Method modeling has been widely used to simulate the distributions of potential, current density, and dissolved species for a wide range of electrochemical systems. The use of the Nernst–Planck–Poisson equations as governing equations is the most rigorous approach, but it brings with it a substantial computational time cost. Reduced-order models offer options in which different assumptions allow more rapid computational solutions. In Part I of this work, it was shown that the loss in accuracy of the reduced-order models is small in high supporting electrolyte concentrations, and there were substantial savings in computational time. The analysis is extended to lower supporting electrolyte concentrations. The most reduced-order modeling approach performs poorly in low supporting electrolyte to electrochemically reactive species (SER) ratios. A modified Laplace approach, in which the conductivity is recalculated at each position at each time step, improves the solution by updating the electrolyte conductivity at each time step, but the errors can still be significant in low SER ratios due to the absence of the diffusion potential term in the calculations.

The Socio-Entrepreneurship Potential of Migrant Workers during the Covid-19 Era: A Mapping Study in Suralaga, East Lombok

The article aims to map the socio-entrepreneurship potential of migrant workers in the Covid-19 era. This mapping is vital to identify the potential that migrant workers have since they seek diversification during the pandemic years. In particular, this paper attempts to investigate the potential of this community as a socio-entrepreneurship resource for migrant workers as it has been seen essential an alternative approach to mitigating the effects of Covid-19. This study focuses on the Suralaga district, an area in East Lombok, which is known for a high number of migrant workers. This study is based on a qualitative approach and phenomenological methodology to gather and analyze data for six months (January–June 2022). There are 25 informants total in this study, 15 of whom are men and 10 of them are women. We used a variety of techniques to gather the data, including participant observation, in-depth interviews, focus groups, and documentation. The results of the study demonstrate that because of their low educational attainment—the majority of migrant employees having only completed elementary or junior high school—migrant workers have limited knowledge and skill, therefore it is difficult to find a job when they finished the contract and back home. In terms of natural potential, the Suralaga region's terrain is part of a farming area, where the majority of people work as farmers and grow tomatoes and chilies. The finding of this research also demonstrates the critical role that micro, small, and medium-sized businesses play in fostering the economic growth of migrant workers and serving as a counterweight to Covid-19's hegemonic middle class.

Total and thermal load forecasting in residential communities through probabilistic methods and causal machine learning

Indoor heating and cooling systems largely influence the power demand of residential buildings and can play a significant role in the Demand Side Management for energy communities. We propose a novel method for probabilistic forecasting of the total load of a residential community and its base and thermal components, combining conformalized quantile regression and causal machine learning techniques, using only aggregate consumption and environmental conditions data. We applied the proposed methods to the dataset of a residential community in Germany, which includes separate measurements of the total electricity demand and of domestic heating system consumption. The results show that the proposed method produces probabilistic day-ahead hourly forecasts of total energy demand that outperform benchmarks and forecasts of electricity consumption components that are not only more accurate than benchmarks but also close to the accuracy achievable with models trained directly on individual load component data. The T-learner method resulted the most effective among the causal methods for load disaggregation in terms of accuracy, simplicity, and potential for extension.

Polarizable Embedding Potentials through Molecular Fractionation with Conjugate Caps Including Hydrogen Bonds.

Polarizable embedding (PE) refers to classical embedding approaches, such as those used in quantum mechanics/molecular mechanics (QM/MM), that allow mutual polarization between the quantum and classical regions. The quality of the embedding potential is critical to provide accurate results, e.g., for spectroscopic properties and dynamical processes. High-quality embedding-potential parameters can be obtained by dividing the classical region into smaller fragments and deriving the parameters from ab initio calculations on the fragments. For solvents and other systems composed of small molecules, the fragments can be individual molecules, while a more complicated fragmentation procedure is needed for larger molecules, such as proteins and nucleic acids. One such fragmentation strategy is the molecular fractionation with conjugate caps (MFCC) approach. As is widely known, hydrogen bonds play a key role in many biomolecular systems, e.g., in proteins, where they are responsible for the secondary structure. In this work, we assess the effects of including hydrogen-bond fragmentation in the MFCC procedure [MFCC(HB)] for deriving the embedding-potential parameters. The MFCC(HB) extension is evaluated on several molecular systems, ranging from small model systems to proteins, directly in terms of molecular electrostatic potentials and embedding potentials and indirectly in terms of selected properties of chromophores embedded in water and complex protein environments.

Anticancer Potential of Novel Nanoemulgel Formulations in Melanoma

Melanoma is classified as one of the most common cancers with an increasing incidence rate and the conventional treatment options that come with undesirable effects decrease the life quality of patients. Herein, as an alternative therapy option for systemic administration, Carbopol-based nanoemulgel formulations for local delivery were designed. Topical drug delivery systems containing Oxaliplatin, a cisplatin derivative anticancer drug used in the treatment of colorectal cancers, were evaluated for their potential for melanoma treatment. Nanoemulgel formulations with particle size under 300 nm were prepared and characterized in terms of droplet size, zeta potential and liquid crystal formation. The viscosity, as a critical attribute for topical drug delivery systems, was also evaluated, and pseudoplastic behavior of these novel drug delivery systems were confirmed. The controlled drug release pattern was shown with in vitro drug release studies with a significant difference from oxaliplatin when applied in solution. In vitro cell viability evaluation with L929 mouse fibroblast cell line confirmed the biocompatibility of prepared formulations, and the anticancer effect of novel nanoemulgel formulations were presented in B16-F10 mouse melanoma cell line. In conclusion, the anticancer potential of Oxaliplatin nanoemulgels were shown in vitro as a therapy option for melanoma, and the advantages of emulsion and gel-based drug delivery systems were combined in a nanotechnology platform for effective and patient-friendly application of an anticancer therapy for melanoma.

Nucleon-Nucleon Elastic Scattering for Motion in The Shifted Deng-Fan Potential

The scattering theory's main objective is to comprehend an object by hurling something at it. One can learn details about an object by observing how it bounces off other objects. The potential that exists between the two particles is the thing that one seeks to comprehend. In time-independent approach to scattering, one assumes that the incident beam has been activated for a very long time and that the entire system is in a stationary state. For short-range local potentials, the variable phase methodology is highly useful in solving quantum mechanical scattering problems. Variable phase methodology/phase-function technique has been explicitly utilized for non-relativistic nucleon-nucleon scattering phenomenon with the fundamental central local potential term and without spin-orbit force. Working under this methodology, scattering phase shifts, total scattering cross section and Differential cross section have been investigated for a new nuclear potential model “Shifted Deng-Fan potential”. Real nucleon-nucleon scattering systems (n-p) and (p-p) have been treated for this purpose with partial waves up to l = 2 in the low and moderate energy region. For l &gt; 0 waves, interacting repulsive barrier potential has been incorporated with the existing central part. Our results for the considered potential model show a close contest with that of the experimental data.

Evaluating the Immune Response of a Nanoemulsion Adjuvant Vaccine Against Methicillin-Resistant Staphylococcus aureus (MRSA) Infection.

Nanoemulsion adjuvant vaccines have attracted extensive attention because of their small particle size, high thermal stability, and ability to induce validly immune responses. However, establishing a series of comprehensive protocols to evaluate the immune response of a novel nanoemulsion adjuvant vaccine is vital. Therefore, this article features a rigorous procedure to determine the physicochemical characteristics of a vaccine (by transmission electron microscopy [TEM], atomic force microscopy [AFM], and dynamic light scattering [DLS]), the stability of the vaccine antigen and system (by a high-speed centrifuge test, a thermodynamic stability test, SDS-PAGE, and western blot), and the specific immune response (IgG1, IgG2a, and IgG2b). Using this approach, researchers can evaluate accuratelythe protective effect of a novel nanoemulsion adjuvant vaccine in a lethal MRSA252 mouse model. With these protocols, the most promising nanoemulsion vaccine adjuvant in terms of effective adjuvant potential can be identified. In addition, the methods can help optimize novel vaccines for future development.

Design of the dual-band shared-aperture asynchronous zoom optical system using focus tunable lenses.

To meet the increasing demand of the current market and the diversity of application scenarios, combine the zoom system and the multi-band shared-aperture system, and fully harness their respective advantages, this paper proposes a dual-band shared-aperture asynchronous zoom optical system using focus tunable lenses (FTLs). To address the lack of available patents for such systems, we designed a sub-system simultaneous iterative optimization algorithm to calculate the initial structure parameters. This synchronous iterative optimization approach can strengthen the connection between sub-systems and compensate for the shortcomings of current mainstream design methods. The initial structure constructed in this way has a good performance in terms of structural stability and optimization potential. Based on these methods, we successfully designed an optical system that can work in both VIS and NIR bands, and the two sub-systems can zoom independently. The design results possess good performance in terms of distortion control, aberration correction, and volume control.

Structural, mechanical, electronic, thermal, and optical properties of the inverse-Heusler compounds X2RuPb(X = La, Sc): A first-principles investigation

Topological insulators are novel quantum material states with insulating bulk band gaps and topologically protected metallic surface states that have been extensively studied owing to their intriguing properties for spintronic and quantum-computing applications. The structural, mechanical, electronic, thermal, and optical properties of inverse Heusler compounds La2RuPb and, Sc2 RuPb in two Hg2CuTi, Cu2 MnAltype structures were calculated using the full potential linear muffin-tin orbital simulation methodology as implemented in the computer code,which is based on density functional theory.We employed the local-density approximation for the exchange and correlation potential (XC) terms. Consequently, the optical characteristics of La2 RuPb, Sc2 RuPb and elastic constants Cij and their corresponding elastic moduli were computed for the first time. According to our structural calculations, La2 RuPb is more stable in its Hg2 CuTi-type structure than Sc2 RuPb in its Cu2 MnAl-type structure. However, the mechanical characteristics demonstrate their stability in the final stages of elastic deformation.

Delivery of florfenicol in veterinary medicine through a PLGA-based nanodelivery system: improving its performance and overcoming some of its limitations.

As is the case with other veterinary antibiotics, florfenicol (FFC) faces certain limitations, such as low solubility in water, or the fact that it is reported to interfere with the immune response after some immunoprofilactic actions in livestock. Aiming to improve its efficacy and overall performance, FFC was loaded into a polymeric nanobased delivery system by succesfully using the emulsion-evaporation technique. The poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with FFC were characterized in terms of size (101 ± 0.52nm), zeta potential (26.80 ± 1.30mV) and poly-dispersity index (0.061 ± 0.019). The achieved loading was 2.24μg FFC/mg of NPs, with an entrapment efficiency of 7.9%. The antimicrobial effect, the anti-biofilm formation and the cytotoxicity properties of the NPs were evaluated. The results indicated a MIC decreased by ~97.13% for S. aureus, 99.33% for E.coli and 64.1% for P. aeruginosa when compared to free FFC. The minimum inhibitory concentration (MIC) obtained indicated the potential for using a significantly lower dose of florfenicol. The delivery system produced biofilm inhibition while showing no cytotoxic effects when tested on porcine primary fibroblasts and horse mesenchymal stem cells. These findings suggest that florfenicol can be improved and formulations optimized for use in veterinary medicine through its incorporation into a nanobased delivery system designed to release in a controlled manner over time.

Genus Abies Mill. (Pinaceae) as the source of plant antimicrobials: A Review

Antimicrobial resistance is one of the major global health problems and it’s related to the enormous number of human deaths. The occurrence and severity of infections caused by microbial pathogens illustrate the need for the identification and characterization of novel antimicrobial agents of natural origin. This review discusses a well-known coniferous genus Abies Mill. in terms of antimicrobial potential. Data regarding the antibacterial, antifungal, and antiviral properties of Abies species were collected and summarized in this review. It was found that 13 different Abies species are recognized as potential sources of antimicrobial compounds. The most investigated species was A. spectabilis (syn. A. webbiana), followed by A. alba, A. cilicica, A. sibirica, A. nordmanniana, A. numidica, A. koreana, A. balsamea, A. holophylla, and A. concolor. Individual studies on A. firma, A. beshanzuensis, and A. cephalonica were also taken into account. The largest number of analyzed results were related to the antibacterial activity of Abies-derived products, but studies on antifungal, and particularly antiviral capacity were also noted. The most investigated products were essential oils and extracts. The broadest antimicrobial activity was observed for A. cilicica. This study noted that some endemic and endangered Abies species were being used for antimicrobial purposes. In that term, the rationalization of the sampling practices and the implementation of the conservation activities are of great importance. This review represents a comprehensive overview of the current knowledge on the antimicrobial potential of the genus Abies.

Horizontal kinetic energy analysis of tropical transition simulations with the WRF and HARMONIE‐AROME models

AbstractFour tropical transition (TT) events in the North Atlantic basin are simulated with the Weather Research and Forecasting (WRF) and the HARMONIE‐AROME (HAR) models to study the main features of the horizontal kinetic energy (HKE) spectra of these kinds of high‐energetic atmospheric system. Though most of the times similar results are obtained with both models, HAR shows a more intense filtering and numerical dissipation, whereas WRF tends to represent overenergized spectra in the synoptic scale and especially at smaller wavelengths. Predictability is dissimilar for the four TTs studied due to the different spectral curve slope obtained for each case, ranging from unlimited to very poor predictability at synoptic scale. Additionally, an increased HKE is presented in the middle–upper troposphere spectra. A deep analysis of the different terms involved in the equation of the spectral energy budget is presented through a detailed study of one of these TTs. The role of all of them is studied, connecting the energy spectra and the meteorological processes involved. The energy budget terms related to the nonlinear spectral transfer, the three‐dimensional divergence, and diabatic process tendencies are identified as the key ones, whereas the potential and kinetic conversion terms and the vertical flux HKE and pressure divergence terms play a secondary role on modulating the spectrum behaviour. The major energetic contributions are found at the synoptic scale, but results show that a two‐dimensional energy cascade does not fully capture the whole spectrum of a TT. The role of convection, latent heat release, and moist convection outbursts is sketched and a link within different vertical levels is found. Results show that a high‐energetic system, such as a TT, can effectively alter the atmospheric energy behaviour.

A comprehensive discussion for the identification of cracking points in f(R) theories of gravity

This paper investigates the concept of cracking and overturning to analyze the impact of local density perturbations on the stability of self-gravitating compact objects in the framework of f(R,phi , X) theory of gravity, where R, phi , and X denote the Ricci scalar, scalar potential, and kinetic term, respectively. In this context, we developed the hydrostatic equilibrium equation for spherically symmetric spacetime with anisotropic matter configuration and subsequently employed the Krori Barua technique. We then perturb the hydrostatic equilibrium state of the configuration by employing the local density perturbation technique, while taking into account the barotropic equation of state. To validate this technique, we employed it on different compact stars namely, Her X-1, SAX J1808.4-3658, 4U 1820-30, PSR J1614-2230, Vela X-1, and Cen X-3, and found that all stars exhibit cracking or overturning for a specific range of model parameters. Conclusively, this study emphasizes that the proposed cracking technique provides significant insights into the stability analysis of self-gravitating compact objects.