Tea is a popular beverage worldwide and also has great medical value. A fundamental understanding of tea shoot growth and a precision picking model should be established to realize mechanized picking of tea shoots with a small product loss. Accordingly, the terminal bud length (Lbud), tea stem length (Lstem), terminal bud angle (αbud), tea stem angle (αstem), and growth time (t) were considered as the key growth parameters; the sum of the vertical lengths of the terminal bud and stem (ξ), the picking radius (r), and the vertical length of the stem (Zstem) were considered as the picking indexes of the tea shoots. The variations in growth parameters with time were investigated using a 3-D coordinate instrument, and the relationships between the growth parameters and the picking indexes were established using an artificial neural network (ANN). The results indicated that the tea growth cycles for periods P1, P2, P3, P4, P5, and P6 were 14, 7, 6, 4, 4, and 6 d, respectively. A growth cycle diagram of the tea growth was established. Moreover, a 5-2-12-3 ANN model was developed. The best prediction of ξ, r, and Zstem was found with 16 training epochs. The MSE value was 0.0923 × 10−4, and the R values for the training, test, and validation data were 0.99976, 0.99871, and 0.99857, respectively, indicating that the established ANN model demonstrates excellent performance in predicting the picking indexes of tea shoots.