Term Preservation Research Articles

Effect of 7-Dehydrocholesterol on Ram Semen Spermatological Parameters on Short Term Preservation

The aim of this study was investigated to effect of 7-dehydrocholesterol loaded cyclodextrin (7-DCLC) on ram semen spermatological parameters on short term preservation at +4 ⁰ C. Collected semen with electroejaculator at non-breeding season. Each animal native ejaculates were mixed and divided into 4 equal groups, diluated with tris/egg yolk extender with included different rates of 7-DCLC (1.5 mg, 3.0 mg and 5 mg/120x10 6 ) and control (C). After extended semen samples were preservation at +4 ⁰ C and evaluated motility, membrane integrity and abnormal spermatozoa rate until 96. hour. There were no significant differences between the groups on abnormal spermatozoa rate (P>0.05); there were a significant differences between the groups on motility at all storage period (P<0.05).The highest motility were observed in 7-DCLC 1.5 mg at 72. and 96. hour (P<0.05). The highest dose (5 mg/120x10 6 ) 7-DCLC were decreased the motility and membrane integrity compared to other groups. Besides, 7-DCLC 3.0 mg group membrane integrity was lower than control at 72. hour (P<0.05). In conclusion, tris/egg yolk extender which is contain 7-DCLC 1.5 mg were protected to ram semen motility and membrane integrity up to 96. hour at +4 ° C preservation.

Gökkuşağı Alabalığı Oncorhynchus mykiss (Walbaum, 1792) Sperminin Kısa Süreli Muhafaza Koşullarına Adaptasyon Sürecinde Kalitelerinin Belirlenmesi

Bu çalışmada, gökkuşağı alabalığı Oncorhynchus mykiss (Walbaum, 1792) spermalarının kısa süreli muhafaza koşullarına adaptasyonu sürecinde sperm kalitesinin incelenmesi ve elde edilen analiz sonuçlarının değerlendirilmesi amaçlanmıştır. Araştırma kapsamında, ortalama 921,50 ± 244,86 g ağırlık ve 41,79 ± 2,98 cm boya sahip erkek damızlıkların ortalama süt verimi 9,56 ± 5,30 ml, sperm konsantrasyonu değişim aralığı 0,27-4,57 × 109 spz.ml-1 olarak ölçülmüştür. Hiçbir koruyucu ya da katkı maddesi kullanılmadan 0-4°C‘de kısa süreli muhafaza edilen spermatozoaların minimum motilite (hareketlilik) kategorileri sınıfına girdikleri belirlenmiştir. Elde edilen veriler ve sonuçlar doğrultusunda Gökkuşağı alabalıkları ile gerçekleştirilecek sperm muhafaza çalışmalarında sperm kalitesi oranları açısından yüksek düzeylere ulaşılabilmesi için üreme dönemine, genetik faktörlere dikkat edilmesi ve üreticilerin kaliteli erkek ve dişilerden oluşan anaç stoğu ve sperm muhafaza birimlerini kendilerinin oluşturmaları, bu tip çalışmaların sürdürülebilirliği ve verimliliği açısından yararlı olacaktır.

Alternative methods for Salt free / Less salt short term preservation of hides and skins in leather making for sustainable development – A review

During the leather processing, large quantities of the salt as sodium chloride, about 30-50 % (% w/w on raw weight) is applied for short term preservation of hides and skins, which subsequently leaches out from the skins/hides and end up in waste streams. This raises a serious environmental concern as well as total dissolved solids (TDS) problem in the wastewater, for which there is no viable treatment method available. Remediation measures such as Reverse Osmosis (RO) or Ultra Filtration (UF) could only separate salt from these waste streams and end up as salt sludge, which necessitates Secured Land Fill (SLF) for disposal option. There are some concerns for SLF as it requires Land area as well as possible leaching due to highly soluble nature of Sodium chloride. Therefore, there is a pressing need for developing an alternative methods for Salt free / Less salt short term preservation of hides and skins. In this regard, Research and Development work is being carried out worldwide and several reports are available. Therefore, it would be beneficial to review and analyze the salt free alternative preservation methods. Even though, some reviews on this topic has been reported earlier, they have not taken into account the patent literature available on this subject. The present paper reviews various alternative methods for Salt free / Less salt short term preservation of hides and skins, taking into account both patent and other publications on this subject.

Surgical Experience and Early Outcomes With a Slim Perimodiolar Electrode.

In 2016 the Cochlear CI532 received FDA approval and has since been the primary full-length electrode from this manufacturer implanted at our center. Our experience to date including surgical technique and early patient outcomes are reviewed here. Since 2016, this array was used as our standard full length Cochlear array, including children with normal anatomy, with 237 total implantations. Surgical experience and clinical outcomes including low frequency hearing preservation and speech perception on CNC words were analyzed in those with at least 6 months follow-up implanted through June 2017 (n = 94). Speech perception scores are improving over clinical follow-up in accord with other electrode arrays. Hearing preservation is possible with a number of patients utilizing acoustic low frequency hearing in conjunction with electric stimulation from their CI. Tip fold overs were infrequent (4.6%) and always identified on intraoperative x-ray. No patients left the operating room with a tip fold over. Speech perception outcomes with the CI532 slim periomodiolar array are similar to other full length arrays with the added potential for at least short term preservation of residual acoustic hearing. There is a learning curve to its use and intraoperative x-rays are valuable to ensure optimal placement.

Diplomática Forense: revisão histórica para a abordagem do documento nato-digital de arquivo

Born digital records massively produced at the end of the 20th century and the beginning of the 21st overpassed the limits of documents produced in the history of humanity, risking both their authenticity and their long term preservation. The aim of the present work intends a historical review of Diplomatic and Digital Forensic Sciences, which allows analyzing how the two types of records in question can contribute to verify the authenticity of the born-digital records. The methodology chosen is of a theoretical nature, since we analyze the main exponents of the mentioned areas and their principal methods of study. It is descriptive, because we mention each one of the most relevant contributions in the historical construction of both sciences. The results of this work allow us to argue that both Diplomatic and Digital Forensic Sciences are complementary and analyze and verify the legal, historical and administrative authenticity of born-digital records. In addition they offer methods to preserve these records in the long run. We conclude that it is relevant to study in detail and depth other areas of knowledge with the interest of providing subsidies to strengthening both Diplomatic and Forensic Science for obtaining a better application in born-digital records.

Associations Between the Principle of Provenance and Metadata: Practical Implementation and Cases

The principle of provenance is the fundamental theory of archives science, which also possesses important reference value in various areas of research and practice. This paper commences with investigating the core value of the principle of provenance, considering the principle to be the unity of theory and method and its core value lies in following historicalism and respecting essential attributes of the archives. Then the authors focus on the realm of government information management and explore the metadata implementation of the core value of the principle of provenance in the era of electronic records. In its essence, the principle of provenance requires full understanding and preservation of the source information of the record. In the age of electronic records this has ascended to the significance of obtaining information, which is often stabilized as metadata in electronic records, reflecting the process of the record’s creation, maintenance and long term preservation. Source information as the contextual information reflecting the record creation process, its typical form of expression in metadata is provenance metadata. Therefore the authors explore the implications of provenance metadata and its essential forms. The authors hold that source information is the indispensable characteristic information in records and archives, and also the essential form for the principle of provenance to exhibit the connotations of provenance. This paper also explains that provenance metadata has further realized the core value of the principle of provenance, playing important roles in ensuring the authenticity, integrity, reliability and usability of electronic records as well as being the basis of their long term preservation and usage. In addition, the authors make concrete exposition on the composition and function of provenance metadata, considering it to be composed of entities and elements, specifically including such metadata as institution, business, environment, relationship and time. Different provenance metadata entities and elements record the source information of the electronic record from its creation until transfer to archives, performing various important functions in government electronic record management. The authors further their research through analyzing and comparing the characteristics of provenance metadata in government electronic record metadata standards at international, national and local jurisdictions levels. Some feasible suggestions for future research are made in the conclusion.

Archeologia e Calcolatori. Accessibilità e diffusione della cultura scientifica

Based on the case study of the journal ‘Archeologia e Calcolatori’, the authors investigate specific issues related to the promotion of Open Science in archaeology. The first part analyses the initiatives undertaken in order to foster the dissemination of the journal’s digital resources on the web, such as the use of descriptive metadata (Dublin Core), the attribution of unique identifiers (DOI), the uploading of the full texts on institutional repositories for long term preservation (CNR-SOLAR), the collaboration with initiatives aiming at the aggregation of cultural and scientific digital contents (MiBACT-CulturaItalia). The second part illustrates many initiatives and projects promoted by the editorial committee to spread the principles of the ‘open access’ philosophy, nationally and internationally. The journal has thus become a record and memory of the progress in the theoretical, as well as applied, aspects of the Open Access movement. This study shows the relevance of the continuous experimentation of the practices for publishing scientific initiatives, adhering to and promoting the Open Access and facilitating the accessibility to its own resources.

Conservation efforts of Grammatophyllum speciosum Blume at Bogor Botanical Garden

ApriliantiP. 2018.Conservation efforts of Grammatophyllum speciosumBlume at Bogor Botanical Garden. Pros Sem Nas Masy Biodiv Indon 4: 261-265.Center for Plant Conservation Botanic Gardens, Lembaga Ilmu Pengetahuan Indonesia (PKT KR-LIPI), known as Bogor Botanic Gardens, is one of the centers for ex-situ plant conservation that has conserved Indonesian flora. One of the most interesting orchid species is Grammatophyllum speciosum Blume or known as sugar cane orchid. This species listed in Government Regulation Number. 7 of 1999 on Preservation of Plants and Animals. The giant size of the plant and inflorescence and beautiful flower made the species threaten in its natural habitat. Conservation effort has been done though, both ex vitro and in vitro as well as its utilization. The plants collected from Sumatera, Kalimantan, Sulawesi, Maluku, dan Papua. In vitro propagation has been conducted using seed from plant collection and small part of propagation product commercialized at Garden Shop in order to disseminate and educate the society about orchid diversity and the importance of ex situ conservation. Synthetic seed technology also applied using protocorm as propagule for short-mid term preservation of the temperature of 4 dan 25°C.

Protocol identification and preservation of myxozoan parasites for microscopy with silver nitrate (Klein's dry) staining technique.

The present study, discusses a first report of staining techniques with silver nitrate (AgNO3 ) stain for the preservation and identification of myxozoans globally. The silver nitrate stain was used to prepare permanent slide preparation of myxozoans with some adaptations made in our laboratory. Fresh air dried smear were stained with silver nitrate stain ensuing dark brown color polar capsules and light brown color of spore-wall. The stain is everlasting for years differing to other stains like Geimsa, iron-heamotoxylin Zeihl-Neelsen Blue, and trichome stains. RESEARCH HIGHLIGHTS: Stains usually fade with time and fail to disclose the morphological characters of the specimen. Present staining method helps to detect less infection in the tissue locating the myxospores. The Klein dry method (1958) is useful and suitable for long term preservation of the myxozoan slides and morphological description.

Long-term preservation of biomedical research data

Genomics and molecular imaging, along with clinical and translational research have transformed biomedical science into a data-intensive scientific endeavor. For researchers to benefit from Big Data sets, developing long-term biomedical digital data preservation strategy is very important. In this opinion article, we discuss specific actions that researchers and institutions can take to make research data a continued resource even after research projects have reached the end of their lifecycle. The actions involve utilizing an Open Archival Information System model comprised of six functional entities: Ingest, Access, Data Management, Archival Storage, Administration and Preservation Planning. We believe that involvement of data stewards early in the digital data life-cycle management process can significantly contribute towards long term preservation of biomedical data. Developing data collection strategies consistent with institutional policies, and encouraging the use of common data elements in clinical research, patient registries and other human subject research can be advantageous for data sharing and integration purposes. Specifically, data stewards at the onset of research program should engage with established repositories and curators to develop data sustainability plans for research data. Placing equal importance on the requirements for initial activities (e.g., collection, processing, storage) with subsequent activities (data analysis, sharing) can improve data quality, provide traceability and support reproducibility. Preparing and tracking data provenance, using common data elements and biomedical ontologies are important for standardizing the data description, making the interpretation and reuse of data easier. The Big Data biomedical community requires scalable platform that can support the diversity and complexity of data ingest modes (e.g. machine, software or human entry modes). Secure virtual workspaces to integrate and manipulate data, with shared software programs (e.g., bioinformatics tools), can facilitate the FAIR (Findable, Accessible, Interoperable and Reusable) use of data for near- and long-term research needs.

Advances in post-harvest technology and value additions of edible mushrooms

Post harvest technology (PHT) is the method applied to the produce after harvest for its protection, processing, distribution, marketing, and utilization to cater the food and nutritional demands of the consumers. Post-harvest losses are very high in most of the food items including mushrooms because of high content of water. Hence, study of the post-harvest physiological processes influencing quality, shelf life and its management to ameliorate the situation is essential. In order to minimize these losses, it is necessary to have adequate and sound post harvest practices for short term/long term storage and processing. Pretreatments, packing, pre cooling are some of the techniques for short term storage of mushrooms while, drying, pickling and steeping preservation are some other techniques commonly used for long term storage of fresh mushrooms. Drying is the most common practice of extending shelf life of mushrooms at room temperatures in rural areas. Sun drying of mushroom is a very old and simple technique to obtain quality products. Sun drying or drying using solar dryers is working very efficiently. Pickling of oyster mushroom is a kind of long term preservation. It is done in a commercial scale by Satpuda Mushroom Sahkari Sangh, an NGO at Hoshangabad district in Madhya Pradesh and Chhattisgarh Mushroom at Raipur (Chhattisgarh). Steeping preservation of mushrooms is a convenient and economical method. In one of the studies clearly indicated that the shelf life of oyster mushroom could be prolonged for a period of 145 days while steeping in 0.1% citric acid, 0.11% acetic acid and 2% salt. In present days, there is a huge demand for the instant or ready made or ready-to-make food products. Now, value addition is becoming an important area and has enormous scope. Value can be added to the mushrooms from grading to the instant made snacks or the main-course item to the varied extent. Several real value-added products like mushroom papad, nuggets, bijoura, chakli, mushroom soup powder, biscuits, mushroom fortified wheat flour (enriched in several nutritional and medicinal attributes), Royal Oyster Capsules and readymade mushroom ketch up have been developed but are yet to be popularized in the Indian market.

Long-term preservation of biomedical research data

Genomics and molecular imaging, along with clinical and translational research have transformed biomedical science into a data-intensive scientific endeavor. For researchers to benefit from Big Data sets, developing long-term biomedical digital data preservation strategy is very important. In this opinion article, we discuss specific actions that researchers and institutions can take to make research data a continued resource even after research projects have reached the end of their lifecycle. The actions involve utilizing an Open Archival Information System model comprised of six functional entities: Ingest, Access, Data Management, Archival Storage, Administration and Preservation Planning. We believe that involvement of data stewards early in the digital data life-cycle management process can significantly contribute towards long term preservation of biomedical data. Developing data collection strategies consistent with institutional policies, and encouraging the use of common data elements in clinical research, patient registries and other human subject research can be advantageous for data sharing and integration purposes. Specifically, data stewards at the onset of research program should engage with established repositories and curators to develop data sustainability plans for research data. Placing equal importance on the requirements for initial activities (e.g., collection, processing, storage) with subsequent activities (data analysis, sharing) can improve data quality, provide traceability and support reproducibility. Preparing and tracking data provenance, using common data elements and biomedical ontologies are important for standardizing the data description, making the interpretation and reuse of data easier. The Big Data biomedical community requires scalable platform that can support the diversity and complexity of data ingest modes (e.g. machine, software or human entry modes). Secure virtual workspaces to integrate and manipulate data, with shared software programs (e.g., bioinformatics tools), can facilitate the FAIR (Findable, Accessible, Interoperable and Reusable) use of data for near- and long-term research needs.

Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells.

A barrier to our understanding of how various cell types and signals contribute to synaptic circuit function is the lack of relevant models for studying the human brain. One emerging technology to address this issue is the use of three dimensional (3D) neural cell cultures, termed 'organoids' or 'spheroids', for long term preservation of intercellular interactions including extracellular adhesion molecules. However, these culture systems are time consuming and not systematically generated. Here, we detail a method to rapidly and consistently produce 3D cocultures of neurons and astrocytes from human pluripotent stem cells. First, pre-differentiated astrocytes and neuronal progenitors are dissociated and counted. Next, cells are combined in sphere-forming dishes with a Rho-Kinase inhibitor and at specific ratios to produce spheres of reproducible size. After several weeks of culture as floating spheres, cocultures ('asteroids') are finally sectioned for immunostaining or plated upon multielectrode arrays to measure synaptic density and strength. In general, it is expected that this protocol will yield 3D neural spheres that display mature cell-type restricted markers, form functional synapses, and exhibit spontaneous synaptic network burst activity. Together, this system permits drug screening and investigations into mechanisms of disease in a more suitable model compared to monolayer cultures.

The Use of Encapsulation-Dehydration Technique for Short-Term Preservation of Endangered Sweet Potato Landraces ( Ipomoea batatas Lam) from Benin

Ex situ conservation of genetic resources remains a challenge for preservation of vegetatively propagated species such as sweet potato. The objective of this study was to establish the protocol based on the encapsulation-dehydration technique for short term preservation of endangered sweet potato landraces produced in Benin. Thus, the evaporative dehydration duration on silicagel was previously determinated on the empty beads which were made using alginate sodium (3%) and calcium chloride (1.32M). Then, the young shoots of two sweet potato landraces (Koidokpon and Dokoui carotte) growing in the screen house were cutted aseptically and desinfected with 10% sodium hypochlorite. The apices were excised on stereoscope and were encapsulated prior their dehydration on silicagel during 5 and 6h. The encapsulated apices were finally conserved in eppendorf tubes at 2°C in batches for 15 days and 90 days. The encapsulated apices were cultured in MS medium supplemented with 0.15 mg/l BAP, 0.2 mg/l NAA, 0.08 mg/l GA3 and 80 mg/l adenine sulfate. The survival and the regeneration rates were then evaluated. At our experimentation condition, the dehydration duration which allowed around 20% water content of the beads was 6h. For the encapsulated apices, the highest survival rates (59.26% and 37.04%) and the highest regeneration rate (37.04% and 11.11%) were recorded respectivelly with the landraces ''Koidokpon'' and ''Dokoui carotte'' when the apices were dehydrated by 6h and stored for 15 days. The regeneration rates decreased according to the stored duration. Significant difference was noted on the regeneration of apices for the landraces tested. This method can be used to preserve the endangered sweet potato landraces and other species during at least three months without subcultures. It also reduce the cost of conservation in terms of consumables and permit better genotype stability during the storage.

Enhancing the Research Data Management of Computer-Based Educational Assessments in Switzerland

Since 2006 the education authorities in Switzerland have been obliged by the Constitution to harmonize important benchmarks in the educational system throughout Switzerland. With the development of national educational objectives in four disciplines an important basis for the implementation of this constitutional mandate was created. In 2013 the Swiss National Core Skills Assessment Program (in German: UGK – Uberprufung der Grundkompetenzen) was initiated to investigate the skills of students, starting with three of four domains: mathematics, language of teaching and first foreign language in grades 2, 6 and 9. UGK uses a computer-based test and a sample size of 25.000 students per year. A huge challenge for computer-based educational assessment is the research data management process. Data from several different systems and tools existing in different formats has to be merged to obtain data products researchers can utilize. The long term preservation has to be adapted as well. In this paper, we describe our current processes and data sources as well as our ideas for enhancing the data management.

Blockchain Governance and The Role of Trust Service Providers: The TrustedChain® Network

Although the blockchain is widely acknowledged as one of the most disruptive technologies emerged in the last decades, many implementation hurdles at the technical, regulatory and governance level still prevent a widespread adoption of services based on open networks. This research discusses the role Trust Service Providers may play in permissioned blockchains, providing a reliable ecosystem in which services can be safely developed and preserved in the long run. As case study, the paper outlines the main features of TrustedChain®, the first blockchain network of European Trust Service Providers specifically designed for highly sensitive sectors, with cutting-edge applications for public administration, e-government, banking, e-health and industry. Emphasis is thus placed on systemic trust, law compliance, adequate technical performance, confidentiality of transactions and long term preservation of data as essential conditions for blockchain networks to thrive and accomplish complex tasks in an effective and reliable way.

Randomised controlled trial comparing the intra-articular hyaluronic acid versus intra-articular steroid in osteoarthritis of knee

&lt;p class="abstract"&gt;&lt;strong&gt;Background:&lt;/strong&gt; Osteoarthritis of knee is a chronic degenerative joint disease which leads to pain, swelling and stiffness. Intra-articular hyaluronic acid or steroids are being used for its treatment. The purpose of the present study was to assess the effect of intra articular hyaluronic acid in comparison to steroids for knee osteoarthritis.&lt;/p&gt;&lt;p class="abstract"&gt;&lt;strong&gt;Methods:&lt;/strong&gt; A prospective randomized study including 70 patients (121 knees) diagnosed with osteoarthritis of knee, were randomly divided into two groups: 35 patients (58 knees) were given intra-articular hyaluronic acid and 35 patients (63 knees) were given intra-articular methylprednisolone. The outcome was evaluated with knee society score (KSS) and visual analog scale (VAS) before the injection and 1, 3 and 6 months after the injection.&lt;strong&gt;&lt;/strong&gt;&lt;/p&gt;&lt;p class="abstract"&gt;&lt;strong&gt;Results:&lt;/strong&gt; The mean KSS pain and function scores increased while VAS decreased in both the groups. All the scores showed improvement at 6 months follow up. In both the groups, the scores at 6 months were significantly better than the pre injection values, and the scores of hyaluronic acid group were significantly better compared to steroid group.&lt;/p&gt;&lt;strong&gt;Conclusions:&lt;/strong&gt; Intraarticular hyaluronic acid seems to be better for long term pain relief and preservation of function in comparison to intraarticular steroids in osteoarthritis of knee.

Collections Support Services (CSS) - 25 Years of Improving Access and Care to our Nation’s Collections

Originally formed in the early 1980s as the Move Crew to move museum collections to the newly opened state of the art Smithsonian Institution’s Museum Support Center, Collections Support Services has evolved into a team of highly skilled museum professionals recognized as trusted experts, innovators, project managers, and problem solvers in all aspects of collections stewardship. We have packed, moved, and stored MILLIONS of objects across Smithsonian museums including the National Museum of Natural History and several of our art museums; the Freer Sackler Gallery, Hirshhorn Museum and Sculpture Garden, and National Museum of African Art. Our vast experience with Natural History collections has been with objects ranging from microscopic invertebrates, fragile bird eggs, 40’ war canoes, whale skulls, giant squids and EVERYTHING in between! Many of these collections came from overcrowded and dusty attics, basements, and warehouses prone to flooding, pest infestation, and poor climate control. We have spent the last 25 years moving these collections into our climate controlled storage pods furnished with new metal cabinets designed for long term preservation. Some of the unique packing and transportation methods we will highlight in this presentation are “airbags” that encompass fragile bird skeletons and uniform shipping containers made of ethafoam planks and old wooden drawers. In addition, we have designed and constructed aluminum pallets for oversized collections, specialized elephant skull pallets, and plaster jackets for paleo fossil specimens. These storage solutions have greatly improved access to collections by allowing researchers to study specimens with minimal handling necessary. This presentation will specifically demonstrate these and other dramatic improvements that we have made as well as highlight innovative solutions we developed to safely transport, store, and provide better access to our Natural History collections for future generations.

Zombie Journals: Designing a Technological Infrastructure for a Precarious Graduate Student Journal

This paper discusses the technical infrastructure and production workflow for the University of Lethbridge Meeting of the Minds Graduate Journal. This infrastructure was designed for an editorial board that anticipated high annual turnover: very easy to understand and train for, able to accommodate differing levels of interest and commitment from year to year, and be “publish and forget”: the long term preservation and discoverability of articles published by the journal had to be ensured regardless of future generations of students’s willingness to support the journal.

Effects of Sodium Bicarbonate on milk preservation

The present investigation was carried out to assess the effect of sodium bicarbonate (NaHCO3) on the keeping quality of raw cow milk. Milk samples were collected from Bangladesh Agricultural University (BAU) Dairy Farm and initial quality were evaluated using physical and chemical tests at Dairy Technology Laboratory of the Department of Dairy Science, BAU. Then, collected samples were preserved at room temperature (28- 30°C) with 0.0 (control), 0.3, 0.4 and 0.5 per cent NaCHO3. The quality of milk samples were measured at every two hours interval up to 6 hours, one hour interval upto 16 hours and thereafter every half an hour interval until spoilage. Initially, colour, flavour and texture of all milk samples were normal (100%), but with progressive storage time colour, flavour and texture of all samples deteriorated gradually, which was more rapid for control samples than that of the NaHCO3 treated samples. Acidity per cent of all samples increased gradually during storage period and the differences in acidity of milk samples in different treatments were significant (p&lt;0.01). Control samples spoiled after 11¼th hours but that of 0.3, 0.4 and 0.5 percent NaHCO3 treated samples spoiled after 16¾th, 17¾th and 18¾th hours,, respectively. It may be concluded that NaHCO3 is the effective chemical neutralizing the acids produced by acid producing bacteria and can be used as a short term preservation of milk under rural condition of Bangladesh where scientific cooling or pasteurization facilities are not available.Res. Agric., Livest. Fish.5(1): 75-85, April 2018