Spinal implants are commonly used in the treatment of spinal disorders or injuries. However, the biomechanical analyses of them are rarely investigated in terms of both biomechanical and clinical perspectives. Therefore, the main purpose of this study is to investigate the effects of rod diameter on the biomechanical behavior of spinal implants and to make a comparison among them. For this purpose, three spinal implants composed of pedicle screws, setscrews and rods, which were manufactured from Ti6Al4V, with diameters of 5.5 mm, 6 mm and 6.35 mm were used and a bilateral vertebrectomy model was applied to spinal systems. Then, the obtained spinal systems were tested under static tension-compression and fatigue (dynamic compression) conditions. Also, failure analyses were performed to investigate the fatigue behavior of spinal implants. After static tension-compression and fatigue tests, it was found that the yield loads, stiffness values, load carrying capacities and fatigue performances of spinal implants enhanced with increasing spinal rod diameter. In comparison to spinal implants with 5.5 mm rods, the fatigue limits of implants showed 13% and 33% improvements in spinal implants having 6 mm and 6.35 mm rods, respectively. The highest static and fatigue test results were obtained from spinal implants having 6.35 mm rods among the tested implants. Also, it was observed that the increasing yield load and stiffness values caused an increase in the fatigue limits of spinal implants.