The wind-resistant performance of cable-stayed bridges under construction deteriorates sharply as the cantilever length increases, and the arrangement of the temporary pier is an effective measure to improve aerodynamic stability. To investigate the effect of the temporary pier on buffeting response of a long-span three-tower cable-stayed bridge under construction, the nonlinear buffeting analysis and wind tunnel test of aeroelastic model methods are adopted in this paper. The influence of the type and arrangement positions of temporary piers on mean wind response and the nonlinear buffeting response of double-cantilever construction are studied, and the arrangement of the temporary pier is optimized based on the buffeting response. The results show that the reduction rates of the RMS of the torsional moment at the tower bottom and the lateral buffeting displacement at the girder end with the rigid pier are 35.6% and 59.2% compared to those without the temporary pier. Further, the buffeting response will decrease as the distance between the rigid pier and the tower increases in the state. On the contrary, in the double-cantilever construction state before the constraint of temporary piers, the buffeting response will become significant as the distance increases. Therefore, it is necessary to consider the buffeting response under two construction states when optimizing the arrangement position of the temporary pier.