In this study, data collected from 2000 to 2019 on vapor pressure deficit (VPD) and its relationship with micrometeorological variables (fire occurrences, aerosol concentration, temperature, and carbon flux) were analyzed in six locations situated in different Brazilian ecosystems: Rio Branco, AC; Manaus, AM; Alta Floresta, MT (within the Amazon Rainforest); Baia das Pedras, MT (Pantanal); Fazenda Miranda, MT (Cerrado); and Petrolina, PE (northeastern semiarid region). Temporal series analysis of VPD was conducted by determining the principal component of singular spectrum analysis (SSA) for this variable in all locations. It was observed that the main component of SSA for VPD is sensitive to local land-use changes, while no evidence of large-scale influences related to global climate change was observed. A strong coupling between VPD values and local maximum temperature with monthly fire occurrence and logarithmic aerosol concentration profiles was also observed. The results of the study are discussed in the context of the ecosystems’ carbon sequestration capacity. The combined results of the study indicate a scenario in which local land-use changes can compromise the capacity of Brazilian ecosystems to absorb carbon.