Temperature Spectra Research Articles

A novel thermotolerant l-rhamnose isomerase variant for biocatalytic conversion of d-allulose to d-allose

A novel l-rhamnose isomerase was identified and cloned from an extreme-temperature aquatic habitat metagenome. The deduced amino acid sequence homology suggested the possible source of this metagenomic sequence to be Chloroflexus islandicus. The gene expression was performed in a heterologous host, Escherichia coli, and the recombinant protein l-rhamnose isomerase (L-RIM) was extracted and purified. The catalytic function of L-RIM was characterized for d-allulose to d-allose bioconversion. d-Allose is a sweet, rare sugar molecule with anti-tumour, anti-hypertensive, cryoprotective, and antioxidative properties. The characterization experiments showed L-RIM to be a Co++- or Mn++-dependent metalloenzyme. L-RIM was remarkably active (~ 80%) in a broad spectrum of pH (6.0 to 9.0) and temperature (70 to 80 °C) ranges. Optimal L-RIM activity with d-allulose as the substrate occurred at pH 7.0 and 75 °C. The enzyme was found to be excessively heat stable, displaying a half-life of about 12 days and 5 days at 65 °C and 70 °C, respectively. L-RIM catalysis conducted at slightly acidic pH of 6.0 and 70 °C achieved biosynthesis of about 30 g L−1 from 100 g L−1d-allulose in 3 h.Key points• The present study explored an extreme temperature metagenome to identify a novel gene that encodes a thermostable l-rhamnose isomerase (L-RIM)• L-RIMexhibits substantial (80% or more) activity in a broad spectrum of pH (6.0 to 9.0) and temperature (70 to 80 °C) ranges• L-RIMis excessively heat stable, displaying a half-life of about 12 days and 5 days at 65 °C and 70 °C, respectively

Quantitative analysis of interfacial microstructural degradation in MCrAlY-coated superalloys during varied service conditions

MCrAlY coatings are extensively utilized to shield turbine blades from oxidative and erosive circumstances. Nonetheless, the interdiffusion between the coating and the superalloy substrate results in the genesis of topologically close-packed (TCP) particles, thereby reducing the longevity of components exposed to high temperatures during operation. In this investigation, the progression of the microstructure in MCrAlY-coated Ni-based single crystal superalloys was meticulously quantified using digital image analysis, considering a spectrum of pre-exposure temperatures, durations, and stress levels. Through digital image analysis, we observed a variation in the length of TCP particles, ranging from 3.25 to 9.8 μm, under different conditions. Interestingly, the width of TCP particles remained consistent at 0.6 μm under all tested conditions. An innovative microstructure forecasting model was formulated, encompassing standardized parameters to evaluate the impact of pre-exposure conditions. This model demonstrated a robust correlation with experimental results. This study contributes to the refinement of our understanding of interfacial microstructure deterioration caused by operational conditions and aids in the progression of coating-substrate chemical compatibility.

State of health (SOH) assessment for LIBs based on characteristic electrochemical impedance

With the continuous development of high energy density cell technology, the electric transport industry proposed higher requirements for the accuracy and real-time performance of automotive lithium batteries (LIBs) health status assessment. A state of health (SOH) assessment is suggested based on electrochemical impedance at characteristic frequency in the study. The distribution of relaxation time is used to deconstruct the internal electrochemical reaction of the battery and the impedance value of electrochemical process is quantified as a feature to avoid the effect of state of charge (SOC) in the evaluation. The imaginary part of impedance feature is applied to characterize the internal temperature of the lithium battery based on the analysis of impedance differences. Thus, the correlation between the state of health and impedance characteristics is examined to obtain the SOH assessment model of lithium battery in the full SOC and wide temperature spectrum. In conclusion, the assessment demonstrates an exceptional goodness-of-fit, with an accuracy of 0.99 in evaluating the SOH of LIBs and internal temperature, thereby indicating the robustness and reliability of the approach.

Wood-Derived Graphite: A Sustainable and Cost-Effective Material for the Wide Range of Industrial Applications

The study explored the graphitization of wood through two distinct methods: a high-temperature approach at 2400 °C and a low-temperature technique at 1400 °C using a catalyst. The graphitization properties were assessed by conducting thermal experiments at various temperatures (1100 °C, 1400 °C, 1800 °C, 2000 °C, and 2400 °C), both with and without a catalyst. The development of graphite lattices was quantitatively analyzed using an array of techniques: X-ray diffractometer (XRD), Raman spectroscopy, high-resolution transmission electron microscopy (HR-TEM), and Fourier transform infrared spectroscopy (FTIR). The XRD analysis highlighted temperature-dependent changes in lattice parameters (d002, La, and Lc), while Raman spectroscopy tracked alterations in the D to G peak ratio (D/G) with temperature. An increase in temperature is correlated with a rise in the number of graphene layers and the degree of graphitization. Notably, the process of graphite lattice formation varied across the experimental temperature spectrum. The use of a catalyst resulted in a reduced d002 spacing, signifying an enhanced degree of graphitization. Moreover, the catalyst promoted a consistent and smooth graphitization process throughout the heating stages. In contrast, graphitization without a catalyst occurred at higher temperatures, specifically between 1800 °C and 2000 °C, with the d002 value stabilizing around 0.338 nm. The catalyst proved instrumental in transforming the initial structure into well-ordered graphite at lower temperatures. This investigation underscores the potential and benefits of employing a catalyst to generate high-quality graphite from wood at reduced temperatures, paving the way for sustainable and economically viable applications of this material.

Soft Robotic Textiles for Adaptive Personal Thermal Management.

Thermal protective textiles are crucial for safeguarding individuals, particularly firefighters and steelworkers, against extreme heat, and for preventing burn injuries. However, traditional firefighting gear suffers from statically fixed thermal insulation properties, potentially resulting in overheating and discomfort in moderate conditions, and insufficient protection in extreme fire events. Herein, an innovative soft robotic textile is developed for dynamically adaptive thermal management, providing superior personal protection and thermal comfort across a spectrum of environmental temperatures. This unique textile features a thermoplastic polyurethane (TPU)-sealed actuation system, embedded with a low boiling point fluid for reversible phase transition, resembling an endoskeleton that triggers an expansion within the textile matrix for enhanced air gap and thermal insulation. The thermal resistance improves automatically from 0.23 to 0.48Km2W-1 by self-actuating under intense heat, exceeding conventional textiles by maintaining over 10°C cooler temperatures. Additionally, the knitted substrate incorporated into the soft actuators can substantially mitigate convective heat transfer, as evidenced by the thermal resistance tests and the temperature mapping derived from numerical simulations. Moreover, it boasts significantly increased moisture permeability. The thermoadaptation and breathability of this durable all-fabric system signify considerable progress in the development of protective clothing with high comfort for dynamic and extreme temperature conditions.

Characterizing the Palm Pathogenic Thielaviopsis Species from Florida.

Thielaviopsis paradoxa sensu lato is a soilborne fungal pathogen that causes Thielaviopsis trunk rot and heart rot in palms. The loss of structural integrity resulting from trunk rot can cause the palm trunk to collapse suddenly and poses a serious threat to life and property. Even though rudimentary knowledge about the Thielaviopsis infection process in palms is available, nothing is known about the T. paradoxa species complex in the US. The aim of this study was to characterize T. paradoxa s. lat. isolates collected from diseased palms grown in Florida. Multi-locus phylogeny using three genes, ITS, β-tubulin, and tef1-α, revealed that the isolates separate into two distinct clades with high bootstrap support. The majority of the isolates clustered with the species T. ethacetica, while two isolates formed a separate clade, distinct from T. musarum, and might represent an undescribed Thielaviopsis species. One representative isolate from each clade, when grown on three distinct media and at four different temperatures, showed differences in gross colony morphology, as well as growth rates. The T. ethacetica isolate TP5448 and the Thielaviopsis sp. isolate PLM300 grew better at opposite ends of the temperature spectrum tested in this study, i.e., 35 °C and 10 °C, respectively. In pathogenicity assays on whole plants, the T. ethacetica isolate proved to be more aggressive than Thielaviopsis sp. isolate PLM300, as it produced larger lesions when inoculated on wounded leaflets. An unequal distribution was observed for the mating-type locus of T. ethacetica, as 12 isolates carried the MAT1-1-1 allele, while the status for four isolates remained undefined. Variation in mycelial growth in response to different fungicides was also observed between the two clades. These results demonstrate the existence of two Thielaviopsis clades that can infect palms in Florida and underscore the need for targeted sampling to help uncover the diversity of Thielaviopsis species across palm-growing regions in the US.

Co-grafting of polyethyleneimine on facilely synthesized mesoporous P(DVB-D) for highly effective CO2 adsorption and enhanced oxidation resistance

Solid amine adsorbents are showing promise as effective adsorbents for the capture of CO2 from various gas mixtures, including biogas and flue exhaust gas. In this study, we introduced a co-grafting strategy using 1,4-butanediol diglycidyl ether (BDDE) on the surface of P(DVB-D). This approach aimed to exploit the reaction involving the opening of epoxy groups with amine groups, forming covalent bonds between polyethyleneimine (PEI) and mesoporous organic polymers, along with the introduction of hydroxy groups to enhance adsorbent long-term stability and antioxidation ability. After an extensive assessment, P(DVB-D)@B-41.2 %PEI exhibited a maximum CO2 capture capacity of 4.53 mmol/g at 50 °C and 50 % RH. Notably, its remarkable CO2 adsorption performance persisted across a diverse temperature spectrum (30–90 °C) and an extensive concentration span (5–30 % vol.). In contrast to conventional adsorbents prepared solely through impregnation methods, P(DVB-D)@B-41.2 %PEI exhibited impressive long-term stability throughout 50 successive cycles and excellent resistance to oxidative degradation. These findings underscore the considerable potential of P(DVB-D)@B-41.2 %PEI in the realm of carbon capture and sequestration (CCS) technology.

The low-cycle fatigue behavior and an entropy-based life prediction model for Nickel-based single crystal superalloy across an extensive temperature range

Nickel-based single-crystal superalloys (Ni-SX) are prominently utilized in the fabrication of turbine blade materials for advanced aero-engines, owing to their commendable material characteristics. This paper delves into the low cycle fatigue (LCF) characteristics of a second-generation Nickel-based single-crystal superalloy across a temperature spectrum ranging from room temperature to 1000 °C. During this procedure, there is an escalation in the trend towards deformation homogenization, leading to a transition in fracture mode from shear fracture to normal fracture. A novel approach is introduced in the form of an entropy-based fatigue life prediction model, grounded in the correlation between cumulative plastic strain and entropy increase rate. Through a comparative examination with experimental data, the model demonstrates proficiency in precisely forecasting the fatigue life of Ni-SX under diverse strain amplitudes and temperature conditions.

Customizing thermometry: Optimizing the operating temperature range of phase transition-based ratiometric luminescence thermometers

Phase transition-based thermometers, which are widely known for their remarkable sensitivity to temperature changes, exhibit a narrow temperature range owing to the stoichiometry of the host material. This limits the applicability of optical sensors utilizing structural phase transitions. In this study, we introduce a co-doping method for modulating the properties of phase transition-based luminescent thermometers to influence the phase transition temperature. We demonstrate that by adjusting the ionic radius of the dopant and its concentration, the transition temperature can be finely tuned across a broad temperature spectrum. The proposed technique enables the customization of luminescent thermometers with enhanced sensitivities and practical temperature ranges tailored to specific user requirements. This study represents a crucial advancement towards the development of personalized luminescence thermometers.

In Situ Observations at the Air‐Sea Interface by Expendable Air‐Deployed Drifters Under Hurricane Michael (2018)

AbstractAn array of surface drifters deployed ahead of Hurricane Michael measured the surface temperature, pressure, directional wind and wave spectra, and surface currents one day before it made landfall as a Category 5 Hurricane. The drifters, 25–50 km apart, spanned two counter‐rotating ocean eddies as Hurricane Michael rapidly intensified. The drifters measured the shift of wave energy between frequency bands in each quadrant of the storm, the response of upper ocean currents, and the resulting cold wake following Michael's passage. Wave energy was greatest in the front quadrants and rapidly decreased in the left‐rear quadrant, where wind and wave energy were misaligned, and components of the wave field were aligned with currents. Hurricane Michael's wave field agreed with previous studies of nondirectional wave spectra across multiple tropical cyclones but had some unique characteristics. The analysis demonstrates how co‐located surface wind and wave observations can complement existing airborne and satellite observations.

Sex differences in body temperature and neural power spectra in response to repeated restraint stress

Repeated stress is associated with an increased risk of developing psychiatric illnesses such as post-traumatic stress disorder (PTSD), which is more common in women, yet the neurobiology behind this sex difference is unknown. Habituation to repeated stress is impaired in PTSD, and recent preclinical studies have shown that female rats do not habituate as fully as male rats to repeated stress, which leads to impairments in cognition and sleep. Further research should examine sex differences after repeated stress in other relevant measures, such as body temperature and neural activity. In this study, we analyzed core body temperature and EEG power spectra in adult male and female rats during restraint, as well as during sleep transitions following stress. We found that core body temperature of male rats habituated to repeated restraint more fully than female rats. Additionally, we found that females had a higher average beta band power than males on both days of restraint, indicating higher levels of arousal. Lastly, we observed that females had lower delta band power than males during sleep transitions on Day 1 of restraint, however, females demonstrated higher delta band power than males by Day 5 of restraint. This suggests that it may take females longer to initiate sleep recovery compared with males. These findings indicate that there are differences in the physiological and neural processes of males and females after repeated stress. Understanding the way that the stress response is regulated in both sexes can provide insight into individualized treatment for stress-related disorders.

Two-Dimensional Spin-Crossover Molecular Solid Solutions with Tunable Transition Temperatures across 90 K.

Spin-crossover (SCO) materials exhibit remarkable potential as bistable switches in molecular devices. However, the spin transition temperatures (Tc) of known compounds are unable to cover the entire ambient temperature spectrum, largely limiting their practical utility. This study reports an exemplary two-dimensional SCO solid solution system, [FeIII(H0.5LCl)2-2x(H0.5LF)2x]·H2O (H0.5LX = 5-X-2-hydroxybenzylidene-hydrazinecarbothioamide, X = F or Cl, x = 0 to 1), in which the adjacent layers are adhered via hydrogen bonding. Notably, the Tc of this system can be fine-tuned across 90 K (227-316 K) in a linear manner by modulating the fraction x of the LF ligand. Elevating x results in strengthened hydrogen bonding between adjacent layers, which leads to enhanced intermolecular interactions between adjacent SCO molecules. Single-crystal diffraction analysis and periodic density functional theory calculations revealed that such a special kind of alteration in interlayer interactions strengthens the FeIIIN2O2S2 ligand field and corresponding SCO energy barrier, consequently resulting in increased Tc. This work provides a new pathway for tuning the Tc of SCO materials through delicate manipulation of molecular interactions, which could expand the application of bistable molecular solids to a much wider temperature regime.

A novel thermophilic strain of Bacillus subtilis with antimicrobial activity and its potential application in solid-state fermentation of soybean meal.

Soybean meal (SBM) is the most important source of plant protein in animal feeds, containing around 41%-48% crude protein. Nevertheless, 70%-80% of these proteins is allergenic antigens that can have adverse implications for the gastrointestinal well-being of animals, especially to young animals. Microbial fermentation is one of the most cost-effective strategies used to reduce allergenic antigens from plant sources. In this study, we report the isolation and characterization of a novel probiotic Bacillus subtilis "L5" strain from lake mud. L5 demonstrated remarkable temperature tolerance across a broad temperature spectrum, thriving at 25°C, 37°C, and 50°C. In addition, antimicrobial assay revealed that L5 exhibits strong antimicrobial activity against Escherichia coli, effectively reducing or eliminating the growth of Gram-negative bacteria in SBM when fermented with L5. When applied to SBM fermentation, L5 efficiently reduced SBM antinutritional factors such as glycinin, β-conglycinin, trypsin inhibitor, phytic acid, neutral detergent fiber, and acid detergent fiber, which in turn results in an increase in crude protein content and the free amino acid concentration. Our findings on the probiotic and fermentation capabilities of L5 suggest that this novel bacterium has dual functions that make it a strong candidate for improving the nutrient values of feed via its role in fermentation.IMPORTANCESoybean meal (SBM), containing 41%-48% crude protein, is the most important source of plant protein in animal feeds. Unfortunately, 70%-80% of the proteins in SBM is allergenic antigens including trypsin inhibition, β-conglycinin, and conglycinin, which negatively affect intestine health and function. Microbial solid-state fermentation methods have been applied to animal feeds for decades, to eliminate antinutritional factors. Here, a novel potential probiotic Bacillus subtilis "L5" strain with high enzymatic activity and antimicrobial activity will be a great help to improve the quality and reproducibility of SBM fermentation.

Extending the glass formation of alkali tellurites

The physical properties of low alkali content tellurite glasses of composition xM2O.(1−x)TeO2 (0≤x≤0·35) have been previously collected in this laboratory. The twin-roller rapid quenching technique (cooling rate>105°C/s), used within a nitrogen environment, successfully and significantly extended the glass-forming range in the lithium and sodium tellurite systems. The upper range for modification has been extended from x=0·35 to 0·80 and up to x=0·625 for the lithium and sodium systems, respectively. In the K, Rb and Cs tellurites, the glass forming ranges were not increased. Glass transition temperature (Tg), crystallisation temperature (Tx), density, molar volume, packing fraction, ionic conductivity and Raman spectra were obtained for these glasses. These results showed structural changes as a function of composition and carbonate retention in the glasses as the amount of alkali modifier increased above x=0·5.

Evidence of strong wave turbulence and of Bolgiano temperature spectra in katabatic winds on steep slopes

The katabatic winds on steep slopes investigated in the present study reveal a novel spectral behavior, observed in the outer part of the jet. At low wavenumbers, the one-dimensional (1D) velocity spectra show evidence of a kx−1 range for the three components of the velocity vector: Eu(kx),Ev(kx),Ew(kx)∝kx−1 [as well as for the 1D temperature spectrum Eθ(kx)∝kx−1]. This suggests the existence of strong wave turbulence. A necessary condition for strong wave turbulence to be manifest is that the flow direction wavenumber, kx, extends to much lower values than the slope normal one, kz. This is satisfied in the present field experiment where wave energy is injected at wavenumber kx=kN=(Na sin α)/uj¯, while kz∼1/Δz, with Na the ambient stratification, α the slope angle, uj¯ the maximum wind velocity, and Δz the shear layer thickness of the jet. In the inertial range, the velocity spectra exhibit a power law kx−5/3 over two decades, whereas the temperature-buoyancy spectra show evidence of a −7/5 slope in the buoyancy sub-range, followed by a −5/3 slope. The change in spectral slopes occurs at the Bolgiano scale LB that is close to the Dougherty–Ozmidov scale LOZ. The high Reynolds number based on the Taylor micro-scale, Reλ∼103, allows clear identification of the spectral laws.

Direct measurement of the inertial-convective subrange above ocean surface waves

The −5/3 power law over the inertial subrange of the turbulence kinetic energy spectrum is one of the most well-known concepts in fluid physics. Obukhov and Corrsin extended the original hypothesis to a passive tracer, leading to the concept of an inertial-convective subrange. These postulates have been empirically validated in the atmospheric turbulence over land but have not been comprehensively studied in the marine air flow. During a recent oceanic campaign, the platform FLIP was deployed with an array of sensors to measure the perturbation wind velocity, temperature, and water vapor. Using these data, a previous study found that Kolmogorov's hypothesized −5/3 was not universally valid over the ocean. Here, we continue that work to analyze the spectrum of temperature and water vapor to empirically evaluate the theoretical extension by Obukhov-Corrsin. For temperature, the observed spectra were too noisy for thorough analysis; our conjecture for the source of noise and its implications for near-surface observations of atmospheric turbulence are discussed. For water vapor, we found strong agreement with the previous analysis of the kinetic energy spectrum. These findings corroborate (1) the theoretical notion of the scalar energy dissipation subrange driven by the inertial motions in the marine boundary layer and (2) evidence for non-Kolmogorov turbulence in the high Reynolds flow immediately above ocean waves. Our analysis shows a strong relationship with distance from the wavy surface; using linear extrapolation, we find that divergence from −5/3 persists in the lowest 25 m of the atmosphere.

Impact of preparation methods on the performance of Cu/Ni/Zr catalysts for methanol decomposition

Utilizing waste heat from engine exhausts to decompose methanol into a hydrogen (H2) and carbon monoxide (CO) mixture, subsequently reintroduced into the engine, offers a significant potential to enhance engine efficiency and reduce emissions. The efficacy of the catalyst is crucial, as it directly influences the composition of the decomposition gases, thereby impacting energy conservation and emissions reduction. This study investigates the impact of various preparation methods for the self-developed Cu/Ni/Zr catalyst for methanol hydrogenation decomposition. These techniques include the co-precipitation method, co-impregnation method, and citrate complexation method, evaluated within a temperature spectrum of 220 °C–320 °C. Employing analytical methods such as x-ray Photoelectron Spectroscopy (XPS), x-ray Diffraction (XRD), Thermogravimetric Analysis-Differential Scanning Calorimetry (TGA-DSC), Brunauer–Emmett–Teller (BET), Temperature-Programmed Reduction (TPR), and Scanning Electron Microscopy (SEM) analysis, the study elucidates the mechanism of methanol decomposition catalyzed by Cu/Ni/Zr. The findings indicate that the catalyst’s activity, in terms of decomposition rate and hydrogen content, ranks in descending order from the co-impregnation method, followed by the citrate complexation method, to the co-precipitation method.

CFD Analysis on HVAC System Functionality in an Amphitheater

Heating, Ventilation and Air conditioning (HVAC) is an important part of residential structures such as single-family homes, apartment buildings, hotels and medium to large industrial and office buildings such as skyscrapers and hospitals, on ships, submarines and in marine environments.This paper investigates the characteristics of the performance HVAC system at “KUET auditorium” regarded as amphitheater for various internal and external conditions. A 2D model of the longitudinal section of the auditorium was drawn by using ANSYS-Fluent software in Design Modeler, for simulating the various functionality of the HVAC system for both summer & winter seasons. In this research, both the indoor & external conditions of KUET auditorium were considered, having the entire spectacle of hall is occupied by people. The main aim of this study was to observe the air temperature, air velocity and relative humidity for each case. The results are represented comparatively as graphs and spectra for various mass flow rates and indoor-outdoor temperatures. It was observed that the HVAC system provided sufficient human comfort conditions in terms of air flow and relative humidty recommended by (American Society of Heating, Refrigerating and Air Conditioing Engineers) AHRAE in the amphitheater for both the summer and winter season.
 Journal of Engineering Science 14(2), 2023, 01-14

Growth and Characterization of Yb: CALYGLO Crystal for Ultrashort Pulse Laser Applications

Yb:CALYGLO crystals with a dopant concentration of 5 at.% were successfully grown using the Czochralski method. The crystal samples were extensively studied to analyze their structure, room temperature and low temperature spectra, and laser properties. The highest absorption cross-section at 977 nm was calculated to be 1.83 × 10−20 cm2 for σ polarization and 5.32 × 10−20 cm2 for π polarization. Similarly, the emission cross-section was determined to be 1.38 × 10−20 cm2 at 980 nm for σ polarization and 2.28 × 10−20 cm2 at 981 nm for π polarization, with a full width at half maximum (FWHM) of 50.3 nm and 89.5 nm, respectively. The fluorescence lifetime of the 5 at.% Yb:CALYGLO crystal at 2F5/2 was measured to be 1.10 ms. Additionally, gain cross-sections were calculated for different β values. In the continuous laser experiment, the crystal demonstrated a laser output of 20.15 W at 1057 nm, with a slope efficiency of 53.3%. These experimental findings indicate that the lattice of Y3+ in the crystal is partially replaced by Lu3+ and Gd3+, resulting in a broader spectrum. Consequently, this crystal shows promising potential as a gain medium for ultrashort pulse laser crystals.

Mapping a Lower Limit on the Mass Fraction of the Cold Neutral Medium Using Fourier-transformed H i 21 cm Emission Line Spectra: Application to the DRAO Deep Field from DHIGLS and the HI4PI Survey

We develop a new method for spatially mapping a lower limit on the mass fraction of the cold neutral medium by analyzing the amplitude structure of Tˆb(kv) , the Fourier transform of T b (v), the spectrum of the brightness temperature of the H i 21 cm line emission with respect to the radial velocity v. This advances a broader effort exploiting 21 cm emission line data alone (without absorption line data, τ) to extract integrated properties of the multiphase structure of the H i gas and to map each phase separately. Using toy models, we illustrate the origin of interference patterns seen in Tˆb(kv) . Building on this, a lower limit on the cold gas mass fraction is obtained from the amplitude of Tˆb at high k v . Tested on a numerical simulation of thermally bi-stable turbulence, the lower limit from this method has a strong linear correlation with the “true” cold gas mass fraction from the simulation for a relatively low cold gas mass fraction. At a higher mass fraction, our lower limit is lower than the “true” value, because of a combination of interference and opacity effects. Comparison with absorption surveys shows a similar behavior, with a departure from linear correlation at N H I ≳ 3–5 × 1020 cm−2. Application to the DRAO Deep Field from DHIGLS reveals a complex network of cold filaments in the Spider, an important structural property of the thermal condensation of the H i gas. Application to the HI4PI survey in the velocity range −90 < v < 90 km s−1 produces a full sky map of a lower limit on the mass fraction of the cold neutral medium at 16.′2 resolution. Our new method has the ability to extract a lower limit on the cold gas mass fraction for massive amounts of emission line data alone with low computing time and memory, pointing the way to new approaches suitable for the new generation of radio interferometers.