Green manure is a biological fertilizer source with complete nutrients, which provides reference for the selection of green manure resistant to low temperature germination. Six green manure crops including Brassica napus L., Orychophragmus violaceus (L.) O. E. Schulz, Medicago sativa L., Lolium perenne L., Melilotus officinalis (Linn.) Pall. and Vicia villosaRoth were selected as experimental materials to study the effects of low temperature stress on germination and seedling growth of different green manure through low temperature germination experiments. The results showed that there were significant differences in cold tolerance of the seeds of 6 medium green manure crops during germination. The germination potential, germination rate, germination index, vitality index, seedling height, root length, seedling fresh weight, soluble protein content and chlorophyll content of Brassica napus L. and Vicia villosaRoth seeds were all better than those of other green manure crops, which provided theoretical basis for the actual production of green manure.