Temperate Grassland Research Articles

Response of soil fungal communities and their co-occurrence patterns to grazing exclusion in different grassland types.

Overgrazing and climate change are the main causes of grassland degradation, and grazing exclusion is one of the most common measures for restoring degraded grasslands worldwide. Soil fungi can respond rapidly to environmental stresses, but the response of different grassland types to grazing control has not been uniformly determined. Three grassland types (temperate desert, temperate steppe grassland, and mountain meadow) that were closed for grazing exclusion for 9 years were used to study the effects of grazing exclusion on soil nutrients as well as fungal community structure in the three grassland types. The results showed that (1) in the 0-5 cm soil layer, grazing exclusion significantly affected the soil water content of the three grassland types (P < 0.05), and the pH, total phosphorous (TP), and nitrogen-to-phosphorous ratio (N/P) changed significantly in all three grassland types (P < 0.05). Significant changes in soil nutrients in the 5-10 cm soil layer after grazing exclusion occurred in the mountain meadow grasslands (P < 0.05), but not in the temperate desert and temperate steppe grasslands. (2) For the different grassland types, Archaeorhizomycetes was most abundant in the montane meadows, and Dothideomycetes was most abundant in the temperate desert grasslands and was significantly more abundant than in the remaining two grassland types (P < 0.05). Grazing exclusion led to insignificant changes in the dominant soil fungal phyla and α diversity, but significant changes in the β diversity of soil fungi (P < 0.05). (3) Grazing exclusion areas have higher mean clustering coefficients and modularity classes than grazing areas. In particular, the highest modularity class is found in temperate steppe grassland grazing exclusion areas. (4) We also found that pH is the main driving factor affecting soil fungal community structure, that plant coverage is a key environmental factor affecting soil community composition, and that grazing exclusion indirectly affects soil fungal communities by affecting soil nutrients. The above results suggest that grazing exclusion may regulate microbial ecological processes by changing the soil fungal β diversity in the three grassland types. Grazing exclusion is not conducive to the recovery of soil nutrients in areas with mountain grassland but improves the stability of soil fungi in temperate steppe grassland. Therefore, the type of degraded grassland should be considered when formulating suitable restoration programmes when grazing exclusion measures are implemented. The results of this study provide new insights into the response of soil fungal communities to grazing exclusion, providing a theoretical basis for the management of degraded grassland restoration.

Anthropogenic shrub encroachment has accelerated the degradation of desert steppe soil over the past four decades

Anthropogenic and natural shrub encroachment have similar ecological consequences on native grassland ecosystems. In fact, there is an accelerating trend toward anthropogenic shrub encroachment, as opposed to the century-long process of natural shrub encroachment. However, the soil quality during the transition of anthropogenic shrub encroachment into grasslands remains insufficiently understood. Here, we used a soil quality assessment method that utilized three datasets and two scoring methods to evaluate changes in soil quality during the anthropogenic transition from temperate desert grassland to shrubland. Our findings demonstrated that the soil quality index decreased with increasing shrub cover, from 0.49 in the desert grassland to 0.31 in the shrubland. Our final results revealed a gradual and significant decline of 36.73 % in soil quality during the transition from desert grassland to shrubland. Reduced soil moisture levels, nutrient availability, and microbial activity characterized this decline. Nearly four decades of anthropogenic shrub encroachment have exacerbated soil drought conditions while leading to a decrease in perennial herbaceous plants and an increase in bare ground cover; these factors can explain the observed decline in soil quality. These findings emphasize the importance of considering soil moisture availability and potential thresholds when implementing revegetation strategies in arid and semiarid regions.

Strengths of fertilizer and litter effects on seedling recruitment and growth of grassland species differ depending on functional groups and seed size.

Agricultural grasslands play an important role in conserving the biodiversity of the European cultural landscape. Both, litter cover and soil nutrient availability, change with grassland management, but it is not well-studied how seedling recruitment and growth of multiple grassland species are influenced by their single or combined effects. Therefore, we studied the effects of nitrogen fertilization (100 kg N per year and ha) and litter cover (250 gdw per m2) on seedling recruitment and growth of 75 temperate grassland species (16 graminoid species, 51 forb species, 8 legume species) in a full factorial microcosm experiment. Overall, fertilizer reduced seedling emergence, while litter cover increased it even when combined with fertilization. Fertilization increased seedling height and biomass, and the combination of fertilizer and litter resulted in even stronger responses. Litter cover alone did not influence seedling biomass or seedling height. While the overall direction of treatment effects was similar across functional groups, their strengths were mostly weaker in graminoids than in non-legume forbs and legumes. Positive litter effects on seedling emergence were stronger in large-seeded species. Positive fertilization effects on seedling growth were stronger in small-seeded species, while their seedling biomass was negatively affected by litter cover. In summary, our results show for multiple grassland species that the combination of litter cover and fertilization modulates their single effects. The varying sensitivity of how grassland species representing different functional groups and seed sizes respond with their seedling emergence and growth to litter cover and nitrogen fertilization indicates that the consequences of land-use change on grassland diversity and composition already start to manifest in the earliest stages of the plant life cycle.

How does soil water retention change over time? A three‐year field study under several production systems

AbstractAgricultural practices and meteorological conditions affect soil structure and soil hydraulic properties. However, their temporal evolution is rarely studied, and even less in the field. Thus, their dynamics are rarely taken into account in models, often leading to inconsistent results and poor decision making. In this study, the temporal evolution of water retention properties and soil structure was monitored over a 3‐year period under several contrasting production systems. Soil Water Retention Curves (SWRCs) obtained directly in the field (with soil water content and potential sensors) were compared with theoretical SWRCs predicted by pedotransfer functions (PTFs) and laboratory SWRCs measured on undisturbed samples. Bulk densities were measured every 2 months. Results indicate a high degree of variability in SWRCs over time and between production systems. The results suggest that variations in the soil water retention behaviour can be induced by crop differentiation, weed control, crop residue management, compaction during harvest, or the introduction of temporary grassland. Contrasting climatic conditions between 2021 (water excess), 2022 (severe drought) and 2023 (intermediate) provided a unique opportunity to study the resilience of the crop systems to extreme climatic conditions. Different soil drying dynamics were observed and some agricultural practices were identified as influencing the soil water retention behaviour for at least 2 years. Comparison of SWRCs showed that the theoretical curves obtained from PTFs are not a good representation of the field SWRCs, especially for less conventional agricultural practices. The laboratory curves are closer with similar trends. However, these SWRCs are not optimal for investigating the temporal evolution of water retention properties. This research also shows that agricultural practices and crops can be levers for contributing to greater food resilience against future climatic conditions. Therefore, to assess the relevance of production systems for tomorrow's needs, studies should focus on the impact of multi‐cropping systems on water retention dynamics in the field.

Multi‐species for multifunctional grassland: Evaluation of minor and novel forage species for temperate humid grasslands

Multi‐species for multifunctional grassland: Evaluation of minor and novel forage species for temperate humid grasslands

Simulation of the nutritional requirements and energy balance of adult cows in a northern temperate grassland.

The forage-livestock balance is an important component of natural grassland management, and realizing a balance between the nutrient energy demand of domestic animals and the energy supply of grasslands is the core challenge in forage-livestock management. This study was performed at the Xieertala Ranch in Hulunbuir City, Inner Mongolia. Using the GRAZPLAN and GrazFeed models, we examined the forage-livestock energy balance during different grazing periods and physiological stages of livestock growth under natural grazing conditions. Data on pasture conditions, climatic factors, supplemental feeding, and livestock characteristics, were used to analyze the metabolizable energy (ME), metabolizable energy for maintenance (MEm), and total metabolizable energy intake (MEItotal) of grazing livestock. The results showed that the energy balance between forage and animals differed for adult cows at different physiological stages. In the early lactation period, although the MEItotal was greater than MEm, it did not meet the requirement for ME. MEItotal was greater than ME during mid-lactation, but there was still an energy imbalance in the early and late lactation periods. In the late lactation period, MEItotal could meet ME requirements from April-September. Adult gestational lactating cows with or without calves were unable to meet their ME requirement, especially in the dry period, even though MEItotal was greater than MEm. Adult cows at different physiological stages exhibited differences in daily forage intake and rumen microbial crude protein (MCP) metabolism, and the forage intake by nonpregnant cows decreased as follows: early lactation > mid-lactation > late lactation, pregnant cows' lactation > dry period. For the degradation, digestion and synthesis of rumen MCP, early-lactation cows were similar to those in the mid-lactation group, but both were higher than those in the late-lactation group, while pregnant cows had greater degradation, digestion, and synthesis of MCP in the lactation period relative to the dry period. For lactating cows, especially those with calves, grazing energy requirements, methane emission metabolism and heat production were highest in August, with increased energy expenditure in winter. Overall, grazing energy, methane emissions and heat production by dry cows were low. In the context of global climate change and grassland degradation, managers must adopt different strategies according to the physiological stages of livestock to ensure a forage-livestock balance and the sustainable utilization and development of grasslands.

Effects of reduced tillage and prolonged cover cropping in maize on soil quality and yield

Maize (Zea mays L.) is the most common arable crop on sandy soils in the Netherlands, and it is often grown for several consecutive years after temporary grassland. For the maize-growing years, reduced tillage intensity and prolonged growing period of cover crops could limit declines in soil organic matter (SOM) and soil biota while maintaining soil structure and maize yield. We measured soil parameters and maize yield in an eight-years field experiment on sandy Gleyic Podzol. Treatments included four cropping systems differing in tillage intensity but with conventional sowing dates (conventional inversion tillage (CT), non-inversion tillage (NIT), strip tillage (ST) and no-till (NT)), and two cropping systems (CT-prolonged and ST-prolonged) with prolonged growing period of a winter rye-winter pea cover crop combined with a late sown short-season maize (18 weeks growth). After eight years, we found no evidence for differences in SOM or total carbon content between the treatments. However, in the reduced-tillage treatments, SOM content was higher in the top (0–15 cm) than in the bottom (15–30 cm) of the plough layer, while in CT and CT-prolonged, this stratification was inverted. We observed differences in earthworm abundance, biomass and functional group distribution. In comparison to CT (100 %), the earthworm abundance was 153 % in NIT, 235 % in ST and 206 % in NT whereas earthworm biomass was 269 % in NIT, 325 % in ST and 475 % in NT. Epigeic earthworms were more affected by tillage than endogeic earthworms. Compared to CT, earthworm biomass was higher in CT-prolonged. The initially low earthworm population recovered during the course of the experiment independent of tillage intensity.Higher soil penetration resistance was observed in the reduced tillage systems compared to CT, independent of prolonging the cover crop. Despite the increased penetration resistance, the maize yields in NIT and ST were similar to CT. The maize yields were lower in NT, CT-prolonged and ST-prolonged compared to CT, but no differences were observed between CT-prolonged and ST-prolonged. The biomass of the harvested prolonged cover crop closed the yield gap in relation to CT in four out of six years in which cover crop biomass in CT-prolonged and ST-prolonged was quantified. Based on our results, we recommend strip tillage for continuous maize-cropping systems on Dutch sandy soils. In the case of severe compacting of compaction prone soils, we recommend conventional tillage with a prolonged cover crop and short-season maize variety.

Effects of intercropping on the herbage production of a binary grass-legume mixture (Hedysarum coronarium L. and Lolium multiflorum Lam.) under artificial shade in Mediterranean rainfed conditions

Growing perennial legumes in the understory layer in agroforestry systems is a strategy to improve the sustainability of agricultural systems, i.e., by increasing land productivity, fostering carbon sequestration and nutrient cycling and reducing reliance on inorganic nitrogen fertilizers for tree crops. In many parts of Central Italy, sulla, a biennial autochthonous legume, is widely adopted in forage cropping systems for its productivity and nutritive value and it is often intercropped with ryegrass for a better utilization as grazed temporary grassland. To evaluate the agronomic performance of these perennial species grown under different levels of light reduction, an artificial shade plot trial was established in the coastal plain of Pisa, Central Italy, with the aim to simulate the effect of tree presence with different levels of shade intensification in rainfed conditions. The experiment layout complies with a 2-way completely randomized design with four replicates. The two factors tested were the forage species (namely sulla, ryegrass and their intercropping) and the level of shade (no shade, moderate shade − 30% light reduction-, and intense shade − 50% light reduction-). Shading significantly reduced the above ground biomass (AGB) production, especially for the intense shading (on average about − 20% with respect to the full sun). The mixture resulted as the most productive crop, yielding on average about + 30 and + 40% AGB in comparison to sulla and ryegrass pure stands, respectively. The findings about the effect of the shading on AGB production showed that mixture was a suitable sward, especially under moderate shading conditions. Further studies are needed with the purpose to investigate the productive performance of these swards in real agroforestry conditions.

Reducing N fertilization in the framework of the European Farm to Fork strategy under global change: Impacts on yields, N2O emissions and N leaching of temperate grasslands in the Alpine region

ContextThe reduction of N fertilization in agriculture as part of the Farm to Fork (F2F) strategy plays a central role in the integrated nutrient management action plan of the European Commission. However, the implications of this strategy for mitigating N losses and possible side-effects on grassland yields under global change are largely unknow. ObjectiveWe examined how a 20% reduction in N fertilization according to the F2F strategy is likely to impact yields, N2O emissions and N leaching of four intensively managed temperate grasslands in the Alpine region, two of them located in Switzerland, the other two in Germany. MethodsFollowing automatic data-driven calibration supported by inverse modeling and a cross-validation step, the process-based model DayCent was used for conducting the analysis. Global change scenarios under the representative concentration pathways (RCPs) 4.5 and 8.5 and a baseline scenario (current climate) were created for the time frame 2041–2060 with the help of the stochastic weather generator LARS-WG. Results and conclusionsOur results indicated that, under current conditions of climate and CO2 levels (400 ppm), a 20% decrease in N fertilization would lead to a 5% drop in yields, but also in a 15% decline in N2O emissions and a 21% decline in N leaching (largely as NO3−). Under global change conditions (i.e., climate change and higher atmospheric CO2 levels), we found that increased yields, mainly induced by higher CO2 levels, are likely to compensate for yield losses resulting from the reduction in N fertilization. In addition, we found that the effectiveness of the F2F strategy to mitigate N losses is likely to be preserved under global change, still with stronger effect on N leaching. The F2F-induced decline in N losses was stronger when the latter were expressed per unit of harvested dry matter, i.e., up to 17% for N2O and up to 42% for N leaching. Although significant, these abatements in N losses are still below the 50% reduction level envisaged by the F2F strategy. Actions related to other axes of the strategy (e.g., sustainable food consumption) will be necessary to further reduce N fertilization and, therefore, to reach this ambitious goal. SignificanceOur results highlight the usefulness of models in accounting for interacting effects of global change and mitigation practices on multiple ecosystem services of grasslands. They allow quantification of the impact of new policies.

Nature-based solutions for effective flood mitigation: potential design criteria

Few studies attempt to measure changes to discharge hydrographs during floods resulting from nature-based Solutions (NbS) for risk mitigation. The Q-NFM project in the UK has sought to measure and compare such changes for a wide range of NbS pilots applied to managed grasslands and woodlands. Also measured were underlying shifts in key hydrological processes leading to flood hydrograph changes of enhanced evaporation, hillslope-, channel- and floodplain-storage, and infiltration. How well particular NbS pilots changed these processes to reduce flood hydrographs was found to depend on the attributes of the NbS features and scheme. This learning is presented for the first time to highlight, with supporting evidence, seven potential criteria to help practitioners of flood risk management to improve existing and future designs of NbS for more effective flood mitigation within temperate grassland and woodland environments.

Distinct variations of soil infiltrability of contrasting root types in a temperate mosaic-pattern grassland in northern China

Soil infiltration capacity is an important factor affecting soil moisture, and is of great significance to plant growth and groundwater recharge in arid and semiarid areas. Patchiness of grasslands widely exist and have a crucial effect on hydrological processes in water-limited regions. This study investigated soil infiltrability under natural grasslands with patchy mosaic patterns based on a series of in-situ tests. Two dominated natural restoration vegetation communities (Bothriochloa ischaemum (B. ischaemum) with fibrous root systems and Artemisia gmelinii (A. gmelinii) with tap root systems) and abandoned land (as control) were selected in the loess hilly and gully region of the Loess Plateau, China. The results showed that natural restoration grasslands significantly improved soil infiltrability in both plant and bare patches, and the emergence of patchy mosaic patterns can result in infiltration heterogeneity to redistribute water on a hillslope scale. Compared to B. ischaemum, A. gmelinii are more likely to facilitate the formation of preferential flow, contributing to the increased soil infiltration rates. The initial, steady and average infiltration rates (IIR, SIR, and AIR, respectively) and soil infiltration capacity index (SIC) were all negatively correlated with both root length density (RLD) and root surface area density (RSAD) of fine roots (diameter < 2 mm) but positively correlated with those of coarse roots (diameter ≥ 2 mm). The main factors influencing the infiltration capacity were the mean weight diameter in the 10−20 cm layer and the RSAD of the fine roots in the 0−20 cm layer, which can explain 98.6 %, 81.7 %, 63.9 %, and 91.3 % of the total variance of IIR, SIR, AIR, and SIC, respectively. This work highlights the important roles of patchy mosaic patterns in affecting soil water redistribution and the stabilization of ecological systems, providing a scientific basis for vegetation selection in the process of vegetation restoration in water-limited regions.

Defoliation modifies the impact of drought on the transfer of recent plant-assimilated carbon to soil and arbuscular mycorrhizal fungi

Abstract Aims The allocation of recent plant photosynthates to soil via arbuscular mycorrhizal (AM) fungi is a critical process driving multiple ecosystem functions in grasslands. Yet, our understanding of how defoliation modifies below-ground allocation of recent plant photosynthate and its response to drought, which is becoming more intense and frequent, remains unresolved. Methods Here we undertook a 13C pulse-labelling experiment in a mesotrophic temperate grassland to evaluate in situ how defoliation intensity modifies the transfer of recently assimilated 13C from plant shoots to roots, extraradical AM fungal hyphae, soil, and 13C-CO2 efflux (soil respiration) in response to simulated drought. Results We found that, individually, both defoliation and drought reduced initial plant 13C uptake, but when defoliation and drought were combined, we detected a significant reduction in below-ground 13C allocation to soil. Furthermore, while defoliation stimulated 13C transfer to plant roots and soil, high intensity defoliation amplified 13C-CO2 efflux relative to the amount of 13C taken up by plants. Drought stimulated 13C transfer to fungal hyphae relative to initial plant uptake. High intensity defoliation, however, suppressed both 13C enrichment of extraradical AM fungal hyphae and 13C transfer to fungal hyphae relative to initial uptake. Conclusions Our findings suggest that defoliation can reduce the transfer of recent photosynthate below-ground under simulated drought and provide new insights into how defoliation may influence grassland C allocation dynamics and cycling between plants and AM fungi in grasslands facing drought.

Divergent changes in particulate and mineral-associated organic carbon under natural revegetation along a soil texture gradient in temperate grasslands of China

The natural revegetation of grasslands can stimulate belowground carbon inputs and facilitate the storage of soil organic carbon (SOC), which is conducive to the restoration of grassland ecosystems. Previous studies have investigated the dynamics of organic carbon (OC) in bulk soil, but paid little attention to OC fractions within aggregates. This study explored the response of OC changes in functionally distinct fractions, namely particulate organic matter (POM) and mineral-associated organic matter (MAOM), to natural restoration through systematic measurements of two grassland vegetation species at three study sites along a soil texture gradient in the Loess Plateau. In naturally restored grasslands, the SOC and OC contents of POM (POM_C) and MAOM (MAOM_C) within bulk soil and aggregates were significantly (p < 0.05) higher relative to bare land, with more effective improvement of POM_C (1.01−6.55 times) than MAOM_C (0.88−4.26 times). Compared to cover type (6.01 %−26.04 %), soil texture explained the variance of OC fractions to a greater extent (56.39 %−88.16 %). Soil texture had a larger contribution to MAOM_C in bulk soil with standardized path coefficients for total effect of 0.78 than POM_C, which had a coefficient of 0.67 (p < 0.05). Moreover, the growth rate of the MAOM_C pool exhibited a gradual decline (p < 0.001), which subsequently approached saturation level despite a persistent increase in total SOC in both bulk soil and aggregates. During soil carbon sequestration, aggregate fractions with relatively smaller particle size reached the MAOM_C saturation more easily than fractions with larger size. The findings of this study highlight the important role of soil texture in determining the saturation level of the MAOM_C pool at the regional scale. In the development of soil carbon sequestration strategies, more attention should be paid to the divergent behaviors of different SOM fractions and their distinct responses to natural restoration at larger spatiotemporal scales.

Extreme drought with seasonal timing consistently promotes CH4 uptake through inconsistent pathways in a temperate grassland, China

Extreme drought with seasonal timing consistently promotes CH4 uptake through inconsistent pathways in a temperate grassland, China

Variation in and Regulation of Carbon Use Efficiency of Grassland Ecosystem in Northern China

Ecosystem carbon use efficiency (CUE) is a key parameter in the carbon cycling of terrestrial ecosystems. The magnitude of CUE reflects the ecosystem’s potential for CO2 sequestration. China’s grasslands play an important role in the carbon cycle. Here, we aimed to investigate the comparation of CUE and its environmental regulation among different grassland in Northern China based on eddy covariance carbon fluxes measurements of 31 grassland sites. The results showed that the average CUE of grassland in Northern China was 0.05 ± 0.22, with a range from −0.42 to 0.66. It was demonstrated that there were significant differences in CUE among different grassland types, and CUE values were ranked by type as follows: alpine grassland &gt; temperate meadow steppe &gt; temperate typical steppe &gt; temperate desert steppe, driven by a combination of climatic, soil, and biological factors, with net ecosystem productivity (NEP) having the greatest impact on them. Except for meadow steppes, moisture had a greater impact on grassland CUE in Northern China than temperature. While temperate desert grassland CUE decreased with increasing soil water content (SWC), the CUE of other grassland types increased with higher precipitation and SWC. These findings will advance our ability to predict future grassland ecosystem carbon cycle scenarios.

The Seasonal Response of N2O Emissions to Increasing Precipitation and Nitrogen Deposition and Its Driving Factors in Temperate Semi-Arid Grassland

The accurate assessment of the rise in nitrous oxide (N2O) under global changes in grasslands has been hindered because of inadequate annual observations. To measure the seasonal response of N2O emissions to increased water and nitrogen (N) deposition, one year round N2O emissions were investigated by chamber weekly in the growing season and every two weeks in the non-growing season in semi-arid temperate grasslands northern China. The results showed the temperate semi-arid grassland to be a source of N2O with greater variability and contribution during the non-growing season. The individual effects of water or N addition increased N2O emissions during the growing season, while the effects of water or N addition depended on the N application rates during the non-growing season. Soil properties, particularly soil temperature and water-filled pore space (WFPS), played key roles in regulating N2O emissions. Structural equation modeling revealed that these factors explained 71% and 35% of the variation in N2O fluxes during the growing and non-growing season, respectively. This study suggested that without observations during the non-growing season it is possible to misestimate the annual N2O emissions and the risk of N2O emissions increasing under global change. This would provide insights for future management strategies for mitigating greenhouse gas emissions.

Environmental drivers of tree species richness in the southernmost portion of the Paranaense forests

BackgroundThe Rio de la Plata grassland region is dominated by temperate grasslands, with the scarce natural forests, influenced floristically by adjacent biogeographical provinces. Uruguay represents the southern limit for many tree species of the Paranaense Province, several of which inhabit the hillside forests. With many species shifting poleward due to climate change, we do not yet know how current environmental factors, particularly climatic ones, are linked to the tree diversity of this flora nowadays. The aim of this study is to understand the geographic pattern of tree richness in the hillside forests of Uruguay, evaluating the water–energy and the environmental heterogeneity hypotheses. The distribution of the hillside forest trees was obtained by compiling and updating the herbaria database and distribution maps of woody plants of Uruguay. The presence/absence of each species, and then the species richness, were georeferenced over a grid that covers Uruguay with 302 cells (660 km2). Over the same grid were compiled environmental variables associated with climate and environmental heterogeneity. The relationship between richness and environmental variables was studied by applying general linear models (GLM). As a strong autocorrelation was detected, a residuals auto-covariate term was incorporated into the GLM, to take into account the species richness spatial structure.ResultsThe tree flora of the hillside forest was composed mainly by Paranaense species that show a latitudinal gradient, with two high richness cores, in the east and northeast of Uruguay. The final model including the environmental variables and the spatial term explained 84% of the variability of tree richness. Species richness showed a positive relationship with precipitation, forest cover, potential evapotranspiration and productivity, while a negative effect of temperature variation was found. The spatial component was the primary predictor, accounting for a 30% of spatial pattern of tree richness.ConclusionsThis study accounts for a large proportion of the environmental and spatial variations of the tree richness pattern of the Paranense flora in its southernmost portion. It brings support to both water–energy and environmental heterogeneity hypotheses, emphasizing the role of climate and its variation and the habitat availability on the hillside forest diversity.

Effects of abiotic restoration through rock addition on invertebrate functional diversity in native temperate grasslands

Invertebrates account for over 90% of all described species and provide crucial ecosystem services. Land clearing, including the removal of abiotic habitat features, threatens invertebrate biodiversity, making environmental restoration crucial for conservation. However, little is known about the effects of abiotic restoration compared to the restoration of biotic features. To determine if rock addition increased invertebrate biodiversity, we introduced 120 t of rocks to 10 sites across five grassland reserves in the Australian Capital Territory in a before‐after‐control‐impact design. We sampled invertebrates using pitfall traps before treatment in 2016 and after rock addition in 2019 (during record‐breaking drought). We recorded 29,164 individual invertebrates from 19 orders and undertook a functional trait‐based analysis of rock effects on the invertebrate community. No effects of rock addition were observed on the probability of occurrence, richness, diversity, community similarity, or abundance of any taxonomic or functional group. The abundance of snare‐building spiders and large ants was higher in the control plots at the end of the experiment. These abundance responses in 2019 did not differ from the control or treatment plots in 2016. Thus, some combination of drought and rock addition appears to have neutralized a positive temporal change that was evident without rocks. Despite a regionwide, replicated restoration effort and an extensive functional analysis of invertebrate communities, very little positive response to rock addition was recorded. Rock addition did not have a negative effect on grassland invertebrate biodiversity and may still be beneficial to the long‐term conservation and restoration of vertebrate and invertebrate communities.

A Contribution to the Flora of Devicolam, Kottayam District, Kerala

This paper deals with the floristic account of Devicolam in Kottayam District, Kerala. The area covered lies between 9° 57'-10° 21' N and 76° 39'-77° 17' E and the altitude varies from 457-2675 m. Five seasonal botanical tours were conducted during 1963-65 and altogether 478 taxa were collected. In addition, 60 species collected by the earlier botanists and which were not collected during the above mentioned tours are also included in this list. Thus the total number of tans enumerated in this paper is 538 and they are spread over 125 families. Different types of vegetation are met with in Devicolam and they can broadly be classified as follows: Tropical dry deciduous, Tropical moist deciduous, Tropical wet evergreen, Montane wet temperate and Montane wet grassland. The occurrence of Argostemma rostratum Wall. in this area constitutes a new record for Peninsular India.

Restoration of open ecosystems in the face of climate change

Open ecosystems occur all around the world in various forms including temperate and tropical grasslands, savannas, shrublands, heathlands, among others. They are home to unique biodiversity, provide key ecosystem services and sustain traditional livelihoods of nearly two billion people. In the face of ongoing climate change, practitioners aiming to restore open ecosystems need the support of the scientific community more than ever. The aim of this Special Issue (SI) is to provide an attention-grabbing collection of high-quality publications addressing the growing challenges of open ecosystems restoration. The SI contains 14 papers that fill various, often interdisciplinary knowledge gaps. Three papers deal with the challenges of identifying the right target states, including the genetic composition of constituting plant species, for restoration under changing environmental conditions and competing stakeholder interests. Five papers advance our understanding on the appropriate timing and methodological toolkit to actively ignite re-assembly of the target plant communities, while two papers focus on situations where spontaneous processes can still also be relied on. The interaction of open ecosystems health and recovery with higher trophic levels, particularly grazers, is also discussed in three papers. Finally, a review paper systematically identifies further knowledge gaps, such as the role of soil microbes in grassland recovery and makes clear guidelines how to fill them. Due to the variety of topics and the rigorous content, this SI provides strong support for open ecosystems restoration policy and practice under the UN Decade on Ecosystem Restoration and beyond.