Temperate Environments Research Articles

Perennial grass and herb options to extend summer–autumn forage in a drought‐prone temperate environment

AbstractBackgroundThe ability to finish livestock on pasture over the summer–autumn period could improve the profitability of red meat enterprises in drought‐prone temperate regions. In south‐eastern Australia, traditional perennial options are limited by poor warm‐season performance (phalaris, Phalaris aquatica L.) and widespread environmental constraints (lucerne, Medicago sativa L.). We aimed to identify perennial species suitable for summer–autumn finishing.MethodsWe tested pure swards of summer‐active perennial grasses and herbs (20 cultivars across 14 species) in replicated small‐plot experiments at two sites on the Southern Tablelands of New South Wales, Australia. We assessed early persistence, productivity and warm‐season nutritive characteristics over 2–3 years.ResultsLucerne and chicory (Cichorium intybus L.) persisted well through drought and produced herbage of high quantity and quality through summer–autumn. Digit grass (Digitaria eriantha Steud.) was highly persistent and productive but nutritive values were generally poor. Cocksfoot (Dactylis glomerata L.), tall fescue (Festuca arundinacea Schreb.), perennial ryegrass (Lolium perenne L.), prairie grass (Bromus willdenowii Kunth.) and plantain (Plantago lanceolata L.) were productive but less persistent through drought, while nutritive values were sometimes inadequate.ConclusionsChicory is a good alternative to lucerne, given its excellent summer–autumn performance, ability to survive droughts and superior acid soil tolerance. If appropriate management resolves issues with persistence and nutritive value, several of the other species could also be used to close the warm‐season feed gap in drought‐prone temperate environments.

Load-Velocity Profiles Before and After Heated Resistance Exercise.

Sweet, DK, Qiao, J, Rosbrook, P, and Pryor, JL. Load-velocity profiles before and after heated resistance exercise. J Strength Cond Res 38(6): 1019-1024, 2024-This study examined neuromuscular performance using load-velocity (L-V) profiles in men and women before and after resistance exercise (RE) in hot (HOT; 40° C) and temperate (TEMP; 21° C) environments. Sixteen (f = 8, m = 8) resistance-trained individuals completed a single 70-minute whole-body high-volume load (6 exercises, 4 sets of 10 repetitions) RE bout in HOT and TEMP. Before and after RE, rectal temperature (TRE), muscle temperature of the vastus lateralis (TVL) and triceps brachii (TTB), and an L-V profile for the deadlift and bench press were recorded. Thermoregulatory and L-V data were analyzed using separate 2-way repeated measures analysis of variances (ANOVAs; condition [hot, temperate] and time [pre, post]) with significance level set at p ≤ 0.05. Deadlift peak velocity was reduced at 60% 1 repetition maximum (1RM) after RE in HOT but not TEMP. Peak velocity of 40% 1RM bench press was lower in TEMP vs. HOT pre-RE (p < 0.01). Peak velocity was decreased at all loads in the deadlift L-V profile after RE, regardless of condition. Despite elevated TRE (TEMP; 37.58 ± 0.35, HOT; 38.20 ± 0.39° C), TVL (TEMP; 35.24 ± 0.62, HOT; 37.92 ± 0.55° C), and TTB (TEMP; 35.05 ± 0.78, HOT; 38.00 ± 0.16° C) after RE in HOT vs. TEMP (p < 0.01), RE in HOT did not broadly affect L-V profiles. This indicates heated resistance exercise can be performed with high-volume load and high ambient temperature with minimal performance impairment.

A chemical weathering and paleoclimatic reconstruction of the early Cambrian environment of the Wyoming Craton from the Wind River Canyon, WY paleosol on the Great Unconformity

The weathering of continental rocks during the Precambrian-Cambrian transition contributed to the carving of the Great Unconformity and coincided with increasing biodiversity during the early Cambrian world. We use an early Cambrian paleosol exposed in the Wind River Canyon of Wyoming where it is buried by the overlying mid-Cambrian Flathead Sandstone to assess the paleo-weathering environment and constrain paleoclimate conditions on the Wyoming Craton in early Cambrian. The ∼ 3 m thick paleo-weathering profile is characterized by complete plagioclase weathering and moderate alkali feldspar weathering with maximum CIA (Chemical Index of Alteration) and PIA (Plagioclase Index of Alteration) reaching 69 and 98, respectively. Higher K concentrations toward the upper part of the profile associated with the authigenic precipitation of sericite clay minerals suggest that modest post-burial K-metasomatism may have overprinted the weathering profile. Our results suggest that the saprolite was developed under a mean annual paleoprecipitation and paleotemperature comparable to modern temperate environments. Geochemical characteristics of this paleosol formed during the final chapter of the Precambrian suggest intensive chemical weathering in the presence of elevated atmospheric CO2 concentration, forming a saprolite profile similar to other, previously described Cambrian and Precambrian-aged paleosols from the North American Craton.

Seagrass mapping across the coast of Tanzania

Seagrasses are marine flowering plants found in both temperate and tropical coastal environments, that offer vital ecosystem services and adaptations. However, due to their proximity to the shore, they are heavily impacted by human activities and climate change, endangering seagrasses and their services. To manage seagrass meadows effectively and sustainably, detailed monitoring and data on distribution and extent are essential. Unfortunately, past under-prioritization of reliable baseline data impedes understanding of ecosystem dynamics and rapid response to declines. This study employs satellite imagery, coupled with ground truthing, to comprehensively report seagrass coverage along Tanzania's mainland coast and Zanzibar islands. By utilizing machine-learning technologies, the study achieves 84.2% accuracy, and found that Tanzania has 2817 km2 of seagrass coverage, which is five times higher than previously reported. In addition, the results also provide coverage of additional benthic habitats, such as corals, rocks/rubble, and sand. This study provides the first comprehensive overview of seagrass coverage in Tanzania and will function as a valuable tool and baseline for understanding and managing these habitats. The findings suggest that seagrasses in Tanzania are still relatively abundant, but due to a number of threats, including pollution, coastal development, and climate change, these habitats require improvement in management strategies including regular monitoring to understand their status and trends in their area coverage. The study highlights the significance of remote sensing and machine learning in mapping and monitoring coastal habitats across extensive geographical areas. We aspire for the presented results to be utilized among stakeholders and researchers to develop more effective conservation measures for seagrasses in Tanzania and the Western Indian Ocean.

Performance of various interpretations of clinical scoring systems for diagnosis of respiratory disease in dairy calves in a temperate climate using Bayesian latent class analysis

Bovine respiratory disease (BRD) presents a challenge to farmers all over the globe, not only because it can have significant impacts on welfare and productivity, but also because diagnosis can prove challenging. Several clinical scoring systems have been developed to aid farmers in making consistent early diagnosis, 2 examples being the Wisconsin (WCS) and the California (CALIF) systems. Neither of these systems were developed in or for use in a temperate environment. As environment may lead to changes in BRD presentation, the weightings and cutoffs designed for one environmental presentation of BRD may not be appropriate when used in a temperate climate. Additionally, the interpretation of the scores recorded varies between studies; this may also influence conclusions. Hence, the objective of this work was to investigate the sensitivity (Se) and specificity (Sp) of these tests in a temperate climate and investigate the influence of varying the interpretation on the performance of the WCS. In this prospective study, 98 commercial spring-calving dairy farms were recruited (40 randomly, 58 targeted) and visited. Thoracic ultrasound and WCS were performed on 20 randomly sampled calves between 4 and 6 wk of age on each farm. On a subset of 32 farms, the CALIF score was also undertaken. The data were then used in a hierarchical Bayesian latent class model to estimate the Se and Sp of 5 different interpretations of the Wisconsin clinical score and 1 interpretation of the California clinical score. In total, 1,936 calves were examined. The Se of the Wisconsin score varied from 0.336 to 0.577 depending on the interpretation used, and the Sp varied from 0.943 to 0.977. The Se of the California score was 0.563 (95% Bayesian credible interval [BCI]: 0.452, 0.681) and the Sp was 0.919 (95% BCI: 0.899, 0.937). In conclusion, the performances of the clinical scores in a temperate environment were similar to previously published work from more extreme climates; however, the performance varied widely depending on the score interpretation. Authors should justify their use of a particular clinical score interpretation to improve clarity in publications.

Trade-offs between winter survival and reproduction in female insects.

In temperate environments most species of insects enter an arrested state of development, known as diapause, that enables them to survive the adverse environmental conditions associated with winter. Although diapause is restricted to a single life stage within species of insects, there are examples of insects that overwinter in the egg, larval, pupal and adult stages. Here we offer a targeted, non-systematic literature review examining how overwintering impacts subsequent reproduction in female insects. Several factors, including the lifestage at which insects overwinter, the type of energy investment strategy females use for breeding, elements of the winter environment, and contributions from male insects can influence trade-offs that female insects face between overwintering survival and post-diapause reproduction. Additionally, climate change and elements of the urban environment, including light pollution and higher temperatures in cities, can exacerbate or ameliorate trade-offs faced by reproducing female insects. Better understanding trade-offs between overwintering survival and reproduction in insects not only enhances our understanding of the underlying physiological mechanisms and ecological processes governing diapause and reproduction, but also provide opportunities to better manage insect pests and/or support beneficial insects.

Effects of dandelion reproduction

This paper mainly explored the seed propagation efficiency of dandelions in different environments and the impact of dandelions on specific environments as a species that easily spreads seeds. It also calculated the seed wind propagation distance as a solution. We incorporated global climate model factors into the first model to explain the factors influencing dandelion dispersal and the ideal range for dandelion seed dispersal in subtropical and temperate environments. Secondly, we used factor analysis to explore whether dandelion is an invasive plant. Expert scoring was used to evaluate whether a species is an invasive plant. In addition, we included two common invasive plants, alfalfa, and American sedge, in the same model and measured them with the same confidence level to conclude. As a result of the above studies, we found that dandelion spreads differently in tropical, subtropical, and temperate zones and is not a serious invasive species. Finally, based on the above conclusions, a suggestion for reasonable cultivation of dandelion was proposed.

Trace gas oxidation sustains energy needs of a thermophilic archaeon at suboptimal temperatures

Diverse aerobic bacteria use atmospheric hydrogen (H2) and carbon monoxide (CO) as energy sources to support growth and survival. Such trace gas oxidation is recognised as a globally significant process that serves as the main sink in the biogeochemical H2 cycle and sustains microbial biodiversity in oligotrophic ecosystems. However, it is unclear whether archaea can also use atmospheric H2. Here we show that a thermoacidophilic archaeon, Acidianus brierleyi (Thermoproteota), constitutively consumes H2 and CO to sub-atmospheric levels. Oxidation occurs across a wide range of temperatures (10 to 70 °C) and enhances ATP production during starvation-induced persistence under temperate conditions. The genome of A. brierleyi encodes a canonical CO dehydrogenase and four distinct [NiFe]-hydrogenases, which are differentially produced in response to electron donor and acceptor availability. Another archaeon, Metallosphaera sedula, can also oxidize atmospheric H2. Our results suggest that trace gas oxidation is a common trait of Sulfolobales archaea and may play a role in their survival and niche expansion, including during dispersal through temperate environments.

Tidal evolution of Earth-like planets in the habitable zone of low-mass stars

Earth-like planets in the habitable zone of low-mass stars undergo strong tidal effects that modify their spin states. These planets are expected to host dense atmospheres that can also play an important role in the spin evolution. On one hand, gravitational tides tend to synchronise the rotation with the orbital mean motion, but on the other hand, thermal atmospheric tides push the rotation away and may lead to asynchronous equilibria. Here, we investigate the complete tidal evolution of Earth-like planets by taking into account the effect of obliquity and eccentric orbits. We adopted an Andrade rheology for the gravitational tides and benchmarked the unknown parameters with the present rotation of Venus. We then applied our model to Earth-like planets, and we show that asynchronous rotation can be expected for planets orbiting stars with masses between 0.4 and 0.9 $M_ and semi-major axes between 0.2 and 0.7 au. Interestingly, we find that Earth-like planets in the habitable zone of stars with masses $ 0.8$ $M_ may end up with an equilibrium rotation of 24 hours. We additionally find that these planets can also develop high obliquities, which may help sustain temperate environments.

Early Pliocene Ostracoda from the Jemmys Point Formation, Gippsland Basin, southeastern Australia: nearshore and offshore origins of biodiversity

Early Pliocene ostracod faunas of the Jemmys Point Formation, onshore Gippsland Basin, yield a rich and well-preserved marine ostracod fauna of mixed shallow marine and deep marine origins. The ostracod faunas evidence a marine continental shelf palaeoenvironment that, during the deposition of one stratigraphic interval, was influenced by a strong, persistent upwelling current. This upwelling current allowed the migration of deep-sea Ostracoda (Philoneptunus sp.) onto the continental shelf. The deeper marine aspect of this early Pliocene fauna, and of modern ostracod faunas from the Bass Strait region, evidence the adaptation of deep shelf taxonomic clades to shallow cool temperate shelf environments and highlights one unusual evolutionary mechanism that has contributed to modern Bass Strait shallow marine biodiversity. Four species are newly described: Neonesidea chapminuta sp. nov., Tasmanocypris salaputia sp. nov., Oculocytheropteron jemmyensis sp. nov., and Philoneptunus plutonis sp. nov. Abbey P. McDonald* [a.mcdonald@deakin.edu.au], School of Life and Environmental Sciences, Deakin University, Burwood, Victoria 3125, Australia; Elizabeth A. Weldon [l.weldon@deakin.edu.au], School of Life and Environmental Sciences and Deakin Marine Research and Innovation Centre, Deakin University, Burwood, Victoria 3125, Australia; Mark T. Warne [mark.warne@deakin.edu.au], School of Life and Environmental Sciences and Deakin Marine Research and Innovation Centre, Deakin University, Burwood, Victoria 3125, Australia, and Museums Victoria, GPO Box 666, Melbourne, Victoria 3001, Australia.

Ambient heat exposure and kidney function in patients with chronic kidney disease: a post-hoc analysis of the DAPA-CKD trial

Higher temperatures are associated with higher rates of hospital admissions for nephrolithiasis and acute kidney injury. Occupational heat stress is also a risk factor for kidney dysfunction in resource-poor settings. It is unclear whether ambient heat exposure is associated with loss of kidney function in patients with established chronic kidney disease. We assessed the association between heat index and change in estimated glomerular filtration rate (eGFR) in participants from the DAPA-CKD trial in a post-hoc analysis. DAPA-CKD was a randomised controlled trial of oral dapagliflozin 10 mg once daily or placebo that enrolled participants aged 18 years or older, with or without type 2 diabetes, with a urinary albumin-to-creatinine ratio of 200-5000 mg/g, and an eGFR of 25-75 mL/min per 1·73 m2. In this post-hoc analysis, we explored the association between time-varying daily centre-level heat index (ERA5 dataset) and individual-level change in eGFR in trial participants using linear mixed effect models and case-time series. The DAPA-CKD trial is registered with ClinicalTrials.gov, NCT03036150. Climate and eGFR data were available for 4017 (93·3%) of 4304 participants in 21 countries (mean age: 61·9 years; mean eGFR: 43·3 mL per 1·73 m2; median 28 months follow-up). Across centres, a heat index of more than 30°C occurred on a median of 0·6% of days. In adjusted linear mixed effect models, within each 120-day window, each 30 days' heat index of more than 30°C was associated with a -0·6% (95% CI -0·9% to -0·3%) change in eGFR. Similar estimates were obtained using case-time series. Additional analyses over longer time-windows showed associations consistent with haemodynamic or seasonal variability, or both, but overall estimates corresponded to an additional 3·7 mL per 1·73 m2 (95% CI 0·1 to 7·0) loss of eGFR per year in a patient with an eGFR of 45 mL per 1·73 m2 located in a very hot versus a temperate environment. Higher ambient heat exposure is associated with more rapid eGFR decline in those with established chronic kidney disease. Efforts to mitigate heat exposure should be tested as part of strategies to attenuate chronic kidney disease progression. None.

Soil water percolation and nutrient fluxes as a function of topographical, seasonal and soil texture variation in Central Amazonia, Brazil

AbstractUnderstanding soil water dynamics and transport of nutrients is challenging in tropical rainforests due to the uniqueness of several properties related to soils, vegetation and seasonality that make relating patterns found in temperate environments to tropical sites difficult. We address the need for better edaphic characterization in tropical environments by investigating soil water percolation rates and chemistry across topographic, soil texture and seasonal gradients in a mature tropical rainforest in Central Amazonia, Brazil. We utilized a passive wick flux meter (e.g., drainage lysimeter) to directly measure real‐time percolation fluxes at 60‐cm depth, and to sample a suite of chemical species across plateau, slope and valley topographic positions. We found percolation flux volume and chemical exports generally increase with decreasing elevation and clay content, which was lowest in the valley. Daily percolation flux was observed to be 2.39 ± 0.44 in plateau, 3.01 ± 0.50 in slope and 6.16 ± 0.83 mm in valley. Most solutes were present in small amounts of &lt;1 mg L−1, such as PO₄3−, Fe2+/Fe3+ and Mn2+; however, NO3− concentrations were &gt;20 mg L−1, even exceeding 100 mg L−1 in the valley. Based on additional isotopic analysis, we speculate high NO3− concentrations are partially an artefact of root decomposition following installation of the flux meters. The empirical relationships we show among percolation volume and nutrient exports under varying topographies and soil textures can improve Earth System Model performance by better constraining ecohydrological relationships to nutrient fluxes, which can in‐turn better illuminate the important factors that govern their behaviour.

Comparative analysis of wheat and barley yield performance across temperate environments

ContextA comparative analysis of grain yield performance of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) under different environments constitutes a way to identify restrictions in yield formation between both crop species. However, most of the previous studies on these field crops have been carried out in Mediterranean environments. ObjectiveThis study aims to compare the grain yield performance of wheat and barley across a wide range of temperate environmental conditions. MethodsWheat and barley yield databases from the national network of cultivar evaluation of Argentina were assembled. The database included 191 wheat cultivars (with early-, mid- and late-flowering length cycle), 140 barley cultivars, and 44 combinations of sites and years of the Argentine wheat and barley belt region. Average heading date variation among environments and crops was no more than 10 days. Crop grain yield comparisons were carried out using a regression analysis, benchmarking within each site-year the grain yield of each cultivar against the average grain yield of all crop cultivars (i.e., environmental index). ResultsThere were no differences in grain yield between wheat and barley for all data, but the behavior between crop species changed with the length of the wheat cycle (i.e., early-, mid-, or late-flowering cultivars). Barley portrayed a greater grain yield than early-flowering wheat under low-yielding environments (< 6060 kg ha−1), but this advantage vanished as the yield environment improved. Mid-flowering wheat showed a similar grain yield to barley across temperate environments. Lastly, late-flowering wheat outperformed barley mainly in high-yielding environments (> 6138 kg ha−1). Grain number m−2 was the main numerical component that explained variability in grain yield. The relative contribution of the yield numerical components differed between species, having barley lower grain number m−2 but a greater grain weight than wheat. ConclusionsThe comparison of the grain yield performance between species represents a strategy to adjust the rotation system, being a critical factor in considering the variability in the crop growth cycle.

Assessment of model projections of climate‐change induced extreme storms on the south‐east coast of Australia

AbstractGeneral circulation models (GCMs) and their downscaled regional‐scale equivalents have been important tools for climate‐change studies. However, there has been limited assessment of the performance of GCMs and downscaled models in simulating extreme storms in temperate coastal environments. This study assesses the model characterization of extreme storms on the heavily populated coast of south‐east Australia. Twenty‐year average recurrence interval (ARI) storm intensities derived from generalized extreme value (GEV) distributions based on observed and large‐scale atmospheric model data are compared. Changes in extreme storms from past climate to a high‐emission future scenario are also investigated. Simulations of storm minimum surface pressures compared favourably with measured data. Both the GCMs and downscaled models reproduced the observed decrease with increasing latitude along the coast in the 20 year ARI of minimum surface pressure. Both indicated that the minimum storm surface pressure should change negligibly in a high‐emission future. Although the models underestimated the maximum daily precipitation significantly, models are improving significantly with CMIP epoch and downscaling. In the high‐emission future scenario, the GCMs and NARCliM projected the 20 year ARI maximum daily precipitation would increase in the order of 25%. GCMs and the corresponding downscaled products presently do not represent the extreme value distributions of historical wind speed data well, overestimating at smaller values of ARI and significantly underestimating in larger values of ARI. Significant changes in the magnitude of the 20 year ARI maximum daily‐average onshore wind speed are not projected for the high‐emission future.

Zoonotic diseases in a ‘Pandemicene’: How can geomorphology contribute to the improved characterisation of dynamic ‘pathogenic landscapes’?

AbstractA zoonosis is an infectious disease that can be transmitted to humans from animals, potentially leading to an epidemic or pandemic. Increasing research into the environmental drivers of zoonoses in a so‐called ‘Pandemicene’ has focused on changes to land use, land cover and climate. But what about the associated changes to earth surface processes, landforms and landscapes that may impact on zoonoses and other infectious diseases with environmental reservoirs (e.g. sapronoses)? This is a crucial question, especially in the context of the Anthropocene, a proposed time interval during which humans have directly and indirectly impacted geomorphological systems and associated abiotic–biotic interactions at local through global scales. Biogeomorphology (and especially zoogeomorphology) potentially has many insights to contribute but is largely divorced from a rapidly expanding body of infectious disease research. By outlining geomorphological principles and providing examples from across dryland, arctic, tropical and temperate environments, we examine the extent to which water, sediment, soil and other organic matter that is directly or indirectly influenced by non‐human animal activity may critically influence the emergence and transmission of infectious diseases. In an era of rapid environmental and socioeconomic change, we contend that geomorphological concepts, data and techniques can play a key role in improved characterisation of dynamic ‘pathogenic landscapes’. We identify several challenges and opportunities that may provide particular foci for enhanced geomorphological contributions to this growing threat to societal resilience, including: (1) working in transdisciplinary ‘One Health’ contexts to test hypotheses about geomorphological controls on pathogen emergence and spread; (2) collaborating with archaeologists to examine past disease dynamics and identify potential future pathogen sources; (3) enhancing existing global maps of infection risk/disease emergence at local to regional scales; and (4) contributing to the robust evidence base needed for policies to monitor and manage infectious disease risk.

Masticatory habits of the adult Neanderthal individual BD 1 from La Chaise-de-Vouthon (France).

The analysis of dental wear provides a useful approach for dietary and cultural habit reconstructions of past human populations. The analysis of macrowear patterns can also be used to better understand the individual chewing behavior and to investigate the biomechanical responses during different biting scenarios. The aim of this study is to evaluate the diet and chewing performance of the adult Neanderthal Bourgeois-Delaunay 1 (BD 1) and to investigate the relationship between wear and cementum deposition under mechanical demands. The macrowear pattern of BD 1 was analyzed using the occlusal fingerprint analysis method. We propose a new method for the bilateral measurement of the cementum volume along both buccal and lingual sides of the molar root. BD 1's anterior dentition is more affected by wear compared to the posterior one. The macrowear pattern suggest a normal chewing behavior and a mixed-diet coming from temperate environments. The teeth on the left side of the mandible display greater levels of wear, as well as the buccal side of the molar crowns. The cementum analysis shows higher buccal volume along the molar roots. BD1 could have been preferably chewing on the left side of the mandible. The exploitation of various food resources suggested by the macrowear analysis is compatible with the environmental reconstructions. Finally, the greater wear on the buccal side of the molar occlusal surface and the greater volume of cementum in that side of the molar roots offers a preliminary understanding about the potential correlation between dental wear and cementum deposition.

Population genomic analyses reveal that salinity and geographic isolation drive diversification in a free-living protist

Protists make up the vast diversity of eukaryotic life and play a critical role in biogeochemical cycling and in food webs. Because of their small size, cryptic life cycles, and large population sizes, our understanding of speciation in these organisms is very limited. We performed population genomic analyses on 153 strains isolated from eight populations of the recently radiated dinoflagellate genus Apocalathium, to explore the drivers and mechanisms of speciation processes. Species of this genus inhabit both freshwater and saline habitats, lakes and seas, and are found in cold temperate environments across the world. RAD sequencing analyses revealed that the populations were overall highly differentiated, but morphological similarity was not congruent with genetic similarity. While geographic isolation was to some extent coupled to genetic distance, this pattern was not consistent. Instead, we found evidence that the environment, specifically salinity, is a major factor in driving ecological speciation in Apocalathium. While saline populations were unique in loci coupled to genes involved in osmoregulation, freshwater populations appear to lack these. Our study highlights that adaptation to freshwater through loss of osmoregulatory genes may be an important speciation mechanism in free-living aquatic protists.

Annual recurrence of prokaryotic climax communities in shallow waters of the North Mediterranean.

In temperate coastal environments, wide fluctuations of biotic and abiotic factors drive microbiome dynamics. To link recurrent ecological patterns with planktonic microbial communities, we analysed a monthly-sampled 3-year time series of 16S rRNA amplicon sequencing data, alongside environmental variables, collected at two stations in the northern Adriatic Sea. Time series multivariate analyses allowed us to identify three stable, mature communities (climaxes), whose recurrence was mainly driven by changes in photoperiod and temperature. Mixotrophs (e.g., Ca. Nitrosopumilus, SUP05 clade, and Marine Group II) thrived under oligotrophic, low-light conditions, whereas copiotrophs (e.g., NS4 and NS5 clades) bloomed at higher temperatures and substrate availability. The early spring climax was characterised by a more diverse set of amplicon sequence variants, including copiotrophs associated with phytoplankton-derived organic matter degradation, and photo-auto/heterotrophic organisms (e.g., Synechococcus sp., Roseobacter clade), whose rhythmicity was linked to photoperiod lengthening. Through the identification of recurrent climax assemblages, we begin to delineate a typology of ecosystem based on microbiome composition and functionality, allowing for the intercomparison of microbial assemblages among different biomes, a still underachieved goal in the omics era.

Diversity of Colletotrichum causing anthracnose on Psidium guajava in varied Brazilian physiographic regions

AbstractAnthracnose, caused by several Colletotrichum species, is a major restricting factor for guava production, but no comprehensive study of the causal agent has been conducted from the plant host centre of diversity. This work characterizes isolates from guava, mainly from the fruit, but also leaves and flowers, representing most Brazilian physiographies according to the partial sequences of the rDNA‐ITS, ApMAT, TUB2, HIS3 and GAPDH gene regions. In addition, the pathogenicity and aggressiveness to fruits of two widely planted guava varieties (SLG and RM) are described. Guava‐derived Colletotrichum isolates were found in five complexes: gloeosporioides, acutatum, boninense, gigasporum and orchidearum. The gloeosporioides complex was the most prevalent (81%), followed by the acutatum complex (14%). A total of 16 Colletotrichum species were naturally associated with guava anthracnose: C. aeschynomenes, C. asianum, C. chrysophilum, C. fructicola, C. gigasporum, C. gloeosporioides, C. karsti, C. melonis, C. musae, C. nymphaeae, C. paranaense, C. siamense, C. sojae, C. syzygicola, C. theobromicola and C. tropicale. Apart from C. nymphaeae and C. gloeosporioides, all the remaining 14 taxa are reported for the first time in P. guajava. The most aggressive species belonged to the C. gloeosporioides complex. C. siamense was the most prevalent, especially in warmer regions, followed by C. chrysophilum, mostly in temperate environments. The most aggressive species were C. siamense, C. chrysophilum, C. fructicola and C. tropicale. Fruits of the variety SLG were consistently more resistant to anthracnose than the fruits of RM.

Atmospheric formaldehyde production on early Mars leading to a potential formation of bio-important molecules

Formaldehyde (H2CO) is a critical precursor for the abiotic formation of biomolecules, including amino acids and sugars, which are the building blocks of proteins and RNA. Geomorphological and geochemical evidence on Mars indicates a temperate environment compatible with the existence of surface liquid water during its early history at 3.8–3.6 billion years ago (Ga), which was maintained by the warming effect of reducing gases, such as H2. However, it remains uncertain whether such a temperate and weakly reducing surface environment on early Mars was suitable for producing H2CO. In this study, we investigated the atmospheric production of H2CO on early Mars using a 1-D photochemical model assuming a thick CO2-dominated atmosphere with H2 and CO. Our results show that a continuous supply of atmospheric H2CO can be used to form various organic compounds, including amino acids and sugars. This could be a possible origin for the organic matter observed on the Martian surface. Given the previously reported conversion rate from H2CO into ribose, the calculated H2CO deposition flux suggests a continuous supply of bio-important sugars on early Mars, particularly during the Noachian and early Hesperian periods.