Technical Demands Research Articles

ANALYSIS OF HIGH-SPEEDRUNNING AND SPRINT RUNNING IN ELITE FEMALE FOOTBALL COMPETITIONS

Introduction: Although football is recognized as the fastest growing sport globally, scientific literature on female footballis still limited. Available published data in understanding the physical demands of female football players have described the necessity of a high level of physical conditioning during matches with average heart rates of 84-86% maximum heart rate and an average of 9.1-11.9 km in total distance covered in the running. The most commonly utilized physical performance measures reported are high-speed running (19km/h-23km/h) and sprinting (>23 km/h). A better understanding of football's physical, technical and tactical demands has resulted from investigations of both training and matches by wearing a global positioning system unit.Objective: This study aimed to assess the running speed and the proportions of different types of running during official competitions in elite female football players.Material and Method: A total of 22 female players (16 seniors and six juniors) that are part of a Romanian First League female football team -Politehnica Timisoara, have been monitored for running speed and covered distance in 6 official matches, which represent a quarter of the championship period. The assessment period was ten weeks (August-October 2021). The monitored parameters (total distance, distance/minute, low speed running, high speed running, sprint running, and maximum speed) wereobtained using K-Sport GPS with a high sampling rate of 50 Hz.Results: During the six analyzed matches the following average values were found: total covered distance -7906.33 ± 1176.68 m, distance/minute -90.83 ± 3.72 m/min, low-speed running distance-7598.50 ± 1102.16 m, high-speed running distance -308 ± 101.31 m, sprint running distance -69.50 ± 28.54 m, and maximum speed -25.13 ± 0.84 km/h.Conclusion: Regarding the monitored parameters (total distance, average speed, proportion of different speed running, maximum speed), we observed a constancy between different matches. During female football matches, high-speed running and sprinting covered 4.77% of the total distance. Based on this data, a future training objective would be the enhancement of this percentage in order to optimize the key moments of the matches.

Position-specific physical and technical demands during the 2019 COPA América Football tournament.

Despite a substantial body of literature on the physical and technical performance characteristics of football players in Asian and European tournaments, research on South American football players is scarce. The purpose of the study was to examine the physical and technical characteristics of football players according to specific playing positions at the 2019 COPA América tournament. A total of 180 match observations from 13 games were monitored using the InStat tracking system. Players were grouped into the following five playing positions: central defenders (n = 45), wide defenders (n = 46), central midfielders (n = 50), wide midfielders (n = 17), and forwards (n = 22). Descriptive statistics (means ± standard deviations) and the one-way analysis of variance were used to analyse the data. Findings showed that the total distance covered by central midfielders (10 553 ± 763 m) was significantly (p < 0.05) higher than that of central defenders (9226 ± 720 m; effect size (d) = 1.79), wide defenders (9929 ± 633 m; d = 0.89) and forwards (9383 ± 820 m; d = 1.45). Wide midfielders (214 ± 170 m), wide defenders (152 ± 199 m) and forwards (138 ± 94 m) covered greater distances sprinting than central defenders (67 ± 42 m; d = 1.19) and central midfielders (91 ± 66 m; d = 0.95). Concerning technical variables, central midfielders played significantly more passes compared to players in other playing positions (p < 0.05). In relation to crossing, wide defenders completed significantly more crosses than players in other positions (p < 0.05). These findings have direct implications for tailoring tactics so players can meet the physical and technical demands of the game.

Minimally invasive approach: is this the future of aortic surgery?

Median sternotomy incision has shown to be a safe and efficacious approach in patients who require thoracic aortic interventions and still represents the gold-standard access. Nevertheless, over the last decade, less invasive techniques have gained wider clinical application in cardiac surgery becoming the first-choice approach to treat heart valve diseases, in experienced centers. The popularization of less invasive techniques coupled with an increased patient demand for less invasive therapies has motivated aortic surgeons to apply minimally invasive approaches to more challenging procedures, such as aortic root replacement and arch repair. However, technical demands and the paucity of available clinical data have still limited the widespread adoption of minimally invasive thoracic aortic interventions. This review aimed to assess and comment on the surgical techniques and the current evidence on mini thoracic aortic surgery.

Aptamer-engineered gold nanorod driven an absorbance enhanced strategy for sensitive biomacromolecule profiling

Gold nanorods (AuNRs)-based plasmonic biosensor offers new opportunity for quantification of biomacromolecules due to their high designability and low technical demands. However, existing methods for the optical detection of biomacromolecule require the targets to induce the aggregation or etching of AuNRs. This limits the range of targets that can be detected, because molecules at extremely low concentration are difficult to arouse aggregation or etching of AuNRs. Thus, it is still challenge to design a scheme for the biomacromolecules at extremely low concentration which can't arouse aggregation or etching of AuNRs based on their plasmonic property. This study proposes a universal absorbance enhanced strategy for biomacromolecule detection with aptamers engineered AuNRs. The biosensor assay (Apts/AuNRs) is designed through assembly of two aptamers on AuNRs to specified recognize the target biomacromolecules, forming closed-loop conformation based on the proximity-dependent ligation, producing absorbance enhancement in the plasmonic peak of AuNRs. It is interesting that the absorbance enhancement increases gradually with increasing protein concentration within a certain range, whereas no aggregation or etching of AuNRs was observed compared with the typical AuNRs based LSPR sensor. Taking advantage of the excellent near infrared light absorption of AuNRs, Apts/AuNRs could be utilized to detect red protein such as cytochrome C, which exhibited better performance than AuNPs based plasmonic sensor. On this basis, the selectivity detection of cytochrome C with the detection of limit down to picomole level was demonstrated. By changing the type of aptamers on AuNRs, the sensitive and credible method was also utilized for the analysis of telomerase activity in nerve cell lysate. Telomerase activity in 4 × 104 neuroblastoma cell was determined to be about 3.575 U/L, which was close to the result of ELISA kit. Good recovery was achieved using standard samples recovery. This study broadens the scope of AuNRs based plasmonic ‬‬‬‬‬‬property ‬‬‬‬and offer a simple, sensitive and selective strategy for biomacromolecules detection in complexed biofluid.

Linear Endobronchial Ultrasound in the Era of Personalized Lung Cancer Diagnostics-A Technical Review.

Major advances in molecular profiling for available targeted treatments and immunotherapy for lung cancer have significantly increased the complexity of tissue-based diagnostics. Endobronchial ultrasound-guided transbronchial needle aspirations (EBUS-TBNA) are commonly performed for diagnostic biopsies and lymph node staging. EBUS-TBNA has increasingly become one of the main sources of tumor cells for molecular analyses. As a result, there is a growing need for high quality EBUS-TBNA samples with adequate cellularity. This has increased the technical demands of the procedure and has created additional challenges, many of which are not addressed in the current EBUS guidelines. This review provides an overview of current evidence on the technical aspects of EBUS-TBNA in light of comprehensive sample processing for personalized lung cancer management. These include sonographic lymph node characterization, optimal needle choice, suction biopsy technique, and the role of rapid on-site evaluation. Attention to these technical details will be important to maximize the throughput of EBUS-TBNA biopsies for molecular testing.

From Compliance to Improvement: How School Leaders Make Sense of Institutional and Technical Demands When Implementing a Continuous Improvement Process

Purpose: A recurring frustration in educational research is the tendency for school leaders to implement reforms in ways that prioritize compliance over more substantive improvements to practice. Drawing on new institutional theory and sensemaking theory, this article explores the different ways leaders respond to continuous improvement (CI) reforms and why they frequently privilege external compliance over the perceived needs of their schools. Methods: This study used interviews, observations, and artifacts to analyze how six leaders across two midwestern school districts led the implementation of a CI method. Data analysis involved an iterative process of identifying emergent themes, refining themes based on existing research, and evaluating their usefulness in explaining differences within and across school leaders, in order to understand the different ways leaders responded to CI and what factors caused them to prioritize compliance over substantive improvement. Findings: Findings illuminate six different responses to CI that vary across three dimensions: whether leaders prioritize bridging or buffering, the form or the function of reform, and concerns for external legitimacy or internal improvement. Leaders’ professional identities, their beliefs about the usefulness of CI, and their perception of district regulation contributed to whether they implemented CI in a way that prioritized concerns for legitimacy over improvement. Implications: These findings trace the shallow reach of recent reform efforts to the ways leaders make sense of the complex institutional and technical demands of their role, offer an integrative typology of leaders’ different approaches to implementation, and identify factors that support more productive responses to district reform.

Measurement of trace bisphenol A in drinking water with combination of immunochromatographic detection technology and SERS method

Sensitive and selective detection of target analyte is very important in many fields such as commodity inspection and quality monitoring. In this work, based on the principle of competitive immunoassay, surface-enhanced Raman spectroscopy (SERS) was used to establish a rapid and highly sensitive method for the detection of trace amounts of bisphenol A in water. Here, Raman molecule 5,5-dithiobis-2-nitrobenzoic acid and anti-BPA antibody were conjugated with Au (core)@Ag (shell) nanoparticle to serve as SERS nanoprobe. After the SERS nanoprobe is combined with the substance to be tested, it uses the siphon effect to pass through the test line and the charging line on the test strip. And the Raman test was performed on the T line with a Raman spectrometer. The detection limitation was 0.1 pg/mL. Compared with the reported gas chromatography, liquid chromatography, fluorescence analysis, and other detection methods, SERS ICA does not demand complicated sample preparation procedures, and has the advantages of simple detection methods, quick results, High sensitivity, good specificity, and low technical demands for laboratory environment and testers. In addition, Raman spectrometers have gradually developed to be portable, making it easier to meet the needs of on-site rapid and highly sensitive detection, and will show broad prospects for applications in the fields of biomedical diagnosis and food safety monitoring.

Ten Years of Universal Testing: How the Rapid Diagnostic Test Became a Game Changer for Malaria Case Management and Improved Disease Reporting.

ABSTRACT.In 2010, the World Health Organization changed its guidance on malaria case management, recommending parasitological confirmation of all suspected cases before treatment with an antimalarial. This recommendation was in large part as a result of the availability of quality assured malaria rapid diagnostic tests (RDTs) that made it possible for malaria diagnosis to be performed by laboratory staff in all health facilities irrespective of the facility’s place in the tiered health system. Community health workers and other non-laboratory health workers who traditionally did not perform malaria testing due to the technical and logistic demands of smear microscopy were now able to test for malaria. The use of RDTs has led to substantial increases in testing rates, improved quality of case management, as well as more accurate reporting of malaria cases. Although current RDTs have limitations, they remain one of the most important tools in contemporary malaria control. Further improvements to existing products, such as increased sensitivity for non-falciparum tests, diversification of Plasmodium falciparum antigen targets, along with strengthened health system support for current RDTs will further enhance their utility in malaria control and prevention.

Aortic arch reconstruction in the Norwood procedure using a curved polytetrafluorethylene patch.

The aortic arch enlargement in the Norwood procedure is classically carried out using a curved homograft patch on the inner curvature of the neoaortic arch. The study investigates the outcome of a newly used artificial patch from a vascular prosthesis as an alternative to a homograft patch. Since April 2007, we used curved polytetrafluorethylene (PTFE) patches cut out of a prosthesis as an alternative to homograft patches for the aortic arch reconstruction. The decision for either patch material was made due to anatomic reasons, preferring PTFE patches in larger aortas. In this study, 224 Norwood patients, operated between April 2007 and April 2018, were analysed. A total of 104 patients received a PTFE patch (group PTFE), and 120 patients got a pulmonary homograft patch (group homograft). A single-centre retrospective analysis was carried out concerning postoperative course and long-term follow-up regarding aortic arch interventions and reoperations and comparing the 2 material groups. There were no material associated operative or postoperative complications. In-hospital mortality was 13% in group PTFE. Six children died late during follow-up (6%). One aortic isthmus dilatation (1%) was carried out 12 months after the Norwood procedure in this group, no arch reoperation was necessary during the complete follow-up. The curved PTFE patch showed good qualities in operative technical demands and excellent long-term results. In selected cases of hypoplastic left heart syndrome, it can be well used as alternative to the pulmonary homograft.

The Influence of Time Winning and Time Losing on Position-Specific Match Physical Demands in the Top One Spanish Soccer League.

The aim of the present study was to analyze the influence of time winning and time losing on position-specific match physical demands with and without ball possession in the top Spanish professional soccer league. All matches played in the First Spanish soccer league over four consecutive seasons (from 2015/16 to 2018/19) were recorded using an optical tracking system (i.e., ChyronHego), and the data were analyzed via Mediacoach®. Total distance (TD), and TD > 21 km·h−1 covered with and without ball possession were analyzed using a Linear Mixed Model, taking into account the contextual variables time winning and losing. Results showed that TD and TD > 21 km·h−1 covered by central midfielders (0.01 and 0.005 m/min, respectively), wide midfielders (0.02 and 0.01 m/min, respectively), and forwards (0.03 and 0.02 m/min, respectively) significantly increased while winning (p < 0.05). By contrast, TD and TD > 21 km·h−1 covered by central defenders (0.01 and 0.008 m/min, respectively) and wide defenders (0.06 and 0.008 m/min, respectively) significantly increased while losing (p < 0.05). In addition, for each minute that teams were winning, total distance with ball possession (TDWP) decreased, while, for each minute that teams were losing, TDWP increased. Instead, TDWP > 21 km·h−1 obtained opposite results. Total distance without ball possession increased when teams were winning, and decreased when teams were losing. Therefore, the evolution of scoreline significantly influences tactical–technical and physical demands on soccer matches.

Upgrading Big Brother: Local Strategic Adaptation in China\u2019s Security Industry

What are the circumstances under which latecomer firms can develop indigenous innovative capacity and compete globally? China’s construction of a vast domestic security apparatus has contributed to it becoming the world’s largest supplier and consumer of video surveillance products and services. It has also produced several globally competitive companies, including those engaged in digital surveillance. Although security equipment producers initially emerged in Guangdong province, China’s leading technology companies are concentrated in Zhejiang province. This comparative study is motivated by the puzzle of why Guangdong, which has a larger security equipment industry with more local investment and earlier introduction of foreign technology, has lagged behind Zhejiang in technological upgrading. We trace this provincial variation to the policy choices of local state bureaucracies and the business strategies of local enterprises. While macro-level variables such as market demand and establishing national industry standards are important for industrial development, this study demonstrates the critical role of local technocrats and entrepreneurs in facilitating technological innovation in a rapidly evolving surveillance regime. Our analysis also reveals how latecomers to a technology-intensive industry were able to adapt their products strategically to meet the technical demands of a major segment of the domestic market, China’s public security sector.

PROFESSIONAL LANGUAGE COMPETENCE OF TECHNICAL UNIVERSITY STUDENT: CONCEPT, STRUCTURE, DEVELOPMENTAL SPECIFICS

The use of competence-based approach in higher technical education demands a certain degree of precision from teachers and researchers when building a structure of a competence. This paper explores foreign language training in a technical university. It discusses the specifics of a concept of competence in the context of a technical university taking into account its qualimetric nature, the ability to measure the results of its development. Also, the concept, structure and developmental specifics of a complex, professional language competence relevant for technical universities are presented. Based on the literature review and the use of method of group expert assessment, the structure of professional language competence for a technical university student is identified. It consists of three its most significant components: communicative, cognitive and domain-specific. With that, the domain-specific component implies two individual educational trajectories which correspond to the needs of future graduates. The presented structure lays the foundation of a pedagogical model and technology developed by the author and can be used for other pedagogical solutions and experiments.

A Commentary on “Can Neuroscience Provide a New Foundation for the Rorschach Variables?” (Jimura et al., 2021)

A Commentary on “Can Neuroscience Provide a New Foundation for the Rorschach Variables?” (Jimura et al., 2021)

Steps to build a DIY low-cost fixed-wing drone for biodiversity conservation.

Despite the proved usefulness of drones in biodiversity studies, acquisition costs and difficulties in operating, maintaining and repairing these systems constrain their integration in conservation projects, particularly for low-income countries. Here we present the steps necessary to build a low-cost fixed-wing drone for environmental applications in large areas, along with instructions to increase the reliability of the system and testing its performance. Inspired by DIY (Do It Yourself) and open source models, this work prioritizes simplicity and accounts for cost-benefit for the researcher. The DIY fixed-wing drone developed has electric propulsion, can perform pre-programmed flight, can carry up to 500 g payload capacity with 65 minutes flight duration and flies at a maximum distance of 20 km. It is equipped with a RGB (Red, Green and Blue) sensor capable of obtaining 2.8 cm per pixel Ground Sample Distance (GSD) resolution at a constant altitude of 100 m above ground level (AGL). The total cost was $995 which is substantially less than the average value of similar commercial drones used in biodiversity studies. We performed 12 flight tests in auto mode using the developed model in protected areas in Brazil, obtaining RGB images that allowed us to identify deforestation spots smaller than 5 m2 and medium-sized animals. Building DIY drones requires some technical knowledge and demands more time than buying a commercial ready-to-fly system, but as proved here, it can be less expensive, which is often crucial in conservation projects.

Ultrasensitive and Multiplexed Protein Imaging with Cleavable Fluorescent Tyramide and Antibody Stripping.

Multiplexed single-cell analysis of proteins in their native cellular contexts holds great promise to reveal the composition, interaction and function of the distinct cell types in complex biological systems. However, the existing multiplexed protein imaging technologies are limited by their detection sensitivity or technical demands. To address these issues, here, we develop an ultrasensitive and multiplexed in situ protein profiling approach by reiterative staining with off-the-shelf antibodies and cleavable fluorescent tyramide (CFT). In each cycle of this approach, the protein targets are recognized by antibodies labeled with horseradish peroxidase, which catalyze the covalent deposition of CFT on or close to the protein targets. After imaging, the fluorophores are chemically cleaved, and the antibodies are stripped. Through continuous cycles of staining, imaging, fluorophore cleavage and antibody stripping, a large number of proteins can be quantified in individual cells in situ. Applying this method, we analyzed 20 different proteins in each of ~67,000 cells in a human formalin-fixed paraffin-embedded (FFPE) tonsil tissue. Based on their unique protein expression profiles and microenvironment, these individual cells are partitioned into different cell clusters. We also explored the cell–cell interactions in the tissue by examining which specific cell clusters are selectively associating or avoiding each other.

A windows technique for sustentaculum tali screw fixation using the sinus tarsi approach for calcaneal fractures: a cadaveric study.

The minimally invasive approach for displaced intra-articular calcaneal fractures is significantly reducing postoperative wound complications. One minimally invasive method, the sinus tarsi approach (STA) has been increasingly widely used. STA is, however, challenging due to its technical demands and the risk of injury to the sural nerve (SN). The purpose of this study was to identify the SN and its branches including their anatomical relationship to the STA as well as to describe an anatomical windows technique for STA including determination of the safe angle for screw insertion into the sustentaculum tali fragment. Thirty-two adult cadaveric legs were disarticulated at the knee and unpaired. STA was performed on each specimen. The anatomy and distribution of the sural nerve and its branches were identified in relation to the incision. Three surgical windows were identified and selected. Kirshner wires were inserted in pairs via each of the windows towards the center of the sustentaculum tali. The safe angle for wire insertion in relation to the SN or its branches was then measured as well as the appropriate intraoperative drilling angle. The plantar branch presented in the distal window in none of the samples, while the dorsal branches presented in 37.5% and the main SN presented in only 6.25%. In the middle window, the dorsal branch presented most often (43.75%) followed by the plantar branch (25.00%) and the SN (21.88%). In the proximal window, the SN presented in 100% of the samples, while the dorsal branch presented in none and the plantar branch presented in about 15.63% of the specimens. All three windows had their own acceptable average angle for screw insertion towards the sustentaculum tali. The distal window is the safest for surgical approach and for calcaneal surgery screw fixation in terms of avoiding sural nerve injury. In addition, that window provides a wide working angle for screw fixation.

Unfolded p53 as a Marker of Oxidative Stress in Mild Cognitive Impairment, Alzheimer's and Parkinson's Disease.

There are several candidate biomarkers for AD and PD which differ in sensitivity, specificity, cost-effectiveness, invasiveness, logistical and technical demands. This study is aimed to test whether plasma concentration of unfolded p53 may help to discriminate among the neurodegenerative processes occurring in Mild Cognitive Impairment, Alzheimer's disease and Parkinson's disease. An electrochemical immunosensor was used to measure unfolded p53 in plasma samples of 20 Mild Cognitive Impairment (13 males/7 females; mean age 74.95±5.31), 20 Alzheimer's (11 males/9 females; mean age: 77.25±7.79), 15 Parkinson's disease patients (12 males/3 females; mean age: 68.60 ± 7.36) and its respective age/sex/studies-matched controls. We observed a significantly higher concentration of unfolded p53 in the plasma of patients of each of the three pathologies with respect to their control groups (p=0.000). Furthermore, the plasma concentration of unfolded p53 was significantly higher in Alzheimer's disease patients in comparison with Mild Cognitive Impairment patients (p=0.000) and Parkinson's disease patients (p=0.006). No significant difference between Mild Cognitive Impairment and Parkinson's disease patients was observed (p=0.524). Our results suggest that unfolded p53 concentration in the plasma may be a useful biomarker for an undergoing neuropathological process that may be common, albeit with different intensity, to different diseases.

Physical, Physiological, and Technical Demands in Ultimate Frisbee Small-Sided Games: Influence of Pitch Size.

Small-sided games (SSGs) are common drills used in various team sports, but the exercise intensity in ultimate Frisbee SSG has not yet been investigated. To clarify the physical, physiological, and technical demands of ultimate Frisbee SSG, we investigated the influence of pitch size on exercise intensity during SSG. Nine male college ultimate Frisbee players played (3 vs. 3) SSG on small (SSGS: 30 × 15 m) and large (SSGL: 40 × 20 m) pitches; both SSGs comprised of four 4 min periods, interspersed by 5 min of passive recovery. Players’ mean heart rate (170 ± 8 and 171 ± 7 bpm), peak heart rate (184 ± 7 and 184 ± 5 bpm), and blood lactate concentration (11.3 ± 4.7 and 11.8 ± 4.6 mmol/L) were similar in SSGS and SSGL, respectively. The total distance covered (1984 ± 166 m and 1702 ± 80 m) and the distance covered during quick (860 ± 112 m and 696 ± 69 m) and high-intensity running (439 ± 95 and 255 ± 44) in SSGL were significantly longer than those in SSGS (p < 0.05). Conversely, the number of accelerations (45 ± 3 and 41 ± 3) and decelerations (44 ± 3 and 40 ± 4), catching errors (2 ± 1 and 1 ± 1), and turnovers (8 ± 2 and 6 ± 2) in SSGS were significantly greater than those in SSGL (p < 0.05). This study suggests that ultimate Frisbee SSG provides high-intensity training, which stimulates the glycolytic pathway. Furthermore, manipulating SSG pitch size effectively modulates the physical demands of SSG.

Professional academy soccer players’ perceived experiences of loan environments

ABSTRACT The current investigation examined professional academy soccer players' perceived experiences of loan environments, compared to their previous academy experiences. Fifteen professional soccer players completed season-long loans at various European soccer clubs. Individual semi-structured interviews were conducted following the loans to determine the physical, technical, tactical, psychological, and psychosocial differences between their loan, and existing academy environments. Inductive content analysis was conducted on the interviews to build a system of categories explaining the holistic experiences of player’s loan experiences. Key themes that emerged were greater perceived physical demands on loan, but similar or lower technical demands. Players were exposed to a wider range of playing styles on loan. Some players struggled with the reduced feedback and communication surrounding their performances on loan compared to the academy, and had difficulty interacting with peers. The current investigation highlights areas for consideration to ensure a smooth transition between academy and loan environments.

CORRELATES OF BODY COMPOSITION AND MOTOR PERFORMANCE VARIABLES OF UNIVERSITY OF ILORIN MALE HANDBALL PLAYERS

AbstractMotor performance is crucial for high-level performance in handball but the individual players' body composition determines the attainment of the enormous physical, psychological, technical and tactical demands required for team success. The study examined the correlates of body composition and motor performance variables of university of Ilorin male handball players. The descriptive correlational research design was adopted. All the 14 University of Ilorin male handball players were purposively selected for the study. Standardised instruments: non-elastic tape rule, weight scale, height scale, cones, whistle, measuring tape and stopwatch were used for data collection. Prior to data collection the weight scale and stopwatch were recalibrated while a pilot study was conducted to ascertain the reliability of the instrument. A Spearman rho of 0.89 was obtained as a confirmation of the instruments reliability. The data was analyzed using percentages, means, standard deviation and Pearson Product Moment Correlation at 0.05 alpha level. The study found moderate to high correlation of body composition with speed, agility and muscular endurance of University of Ilorin handball players. It was recommended that periodic assessment of body composition of the University of Ilorin male handball team should be included as part of the training requirement in other to maintain normal fat mass and fat-free mass for high-level performance.Key words: Fat mass, Whistle, Weight scale, Body Composition