Aptamer-engineered gold nanorod driven an absorbance enhanced strategy for sensitive biomacromolecule profiling

Abstract

Gold nanorods (AuNRs)-based plasmonic biosensor offers new opportunity for quantification of biomacromolecules due to their high designability and low technical demands. However, existing methods for the optical detection of biomacromolecule require the targets to induce the aggregation or etching of AuNRs. This limits the range of targets that can be detected, because molecules at extremely low concentration are difficult to arouse aggregation or etching of AuNRs. Thus, it is still challenge to design a scheme for the biomacromolecules at extremely low concentration which can't arouse aggregation or etching of AuNRs based on their plasmonic property. This study proposes a universal absorbance enhanced strategy for biomacromolecule detection with aptamers engineered AuNRs. The biosensor assay (Apts/AuNRs) is designed through assembly of two aptamers on AuNRs to specified recognize the target biomacromolecules, forming closed-loop conformation based on the proximity-dependent ligation, producing absorbance enhancement in the plasmonic peak of AuNRs. It is interesting that the absorbance enhancement increases gradually with increasing protein concentration within a certain range, whereas no aggregation or etching of AuNRs was observed compared with the typical AuNRs based LSPR sensor. Taking advantage of the excellent near infrared light absorption of AuNRs, Apts/AuNRs could be utilized to detect red protein such as cytochrome C, which exhibited better performance than AuNPs based plasmonic sensor. On this basis, the selectivity detection of cytochrome C with the detection of limit down to picomole level was demonstrated. By changing the type of aptamers on AuNRs, the sensitive and credible method was also utilized for the analysis of telomerase activity in nerve cell lysate. Telomerase activity in 4 × 104 neuroblastoma cell was determined to be about 3.575 U/L, which was close to the result of ELISA kit. Good recovery was achieved using standard samples recovery. This study broadens the scope of AuNRs based plasmonic ‬‬‬‬‬‬property ‬‬‬‬and offer a simple, sensitive and selective strategy for biomacromolecules detection in complexed biofluid.