PDF HTML阅读 XML下载 导出引用 引用提醒 中草药淫羊藿和菟丝子对日本鳗鲡卵巢发育的影响 DOI: 作者: 作者单位: 1. 集美大学水产学院, 福建 厦门 361021;2. 鳗鲡现代产业技术教育部工程研究中心, 福建 厦门 361021;3. 厦门大学海洋与地球学院, 福建 厦门 361102;4. 厦门出入境检验检疫局检验检疫技术中心, 福建 厦门 361026 作者简介: 赖晓健(1984-),男,博士,硕士生导师,主要从事鱼类生殖生理学研究.E-mail:laixj@jmu.edu.cn 通讯作者: 中图分类号: S965 基金项目: 国家自然科学基金项目(41806193);厦门市科技局项目(3502Z20133015);鳗鲡现代产业技术教育部工程研究中心开放基金项目(RE201604,RE201503). Chinese herbs Epimedium brevicornum Maxim. and Cuscuta chinensis Lam. induced ovary development in Japanese eel Anguilla japonica Author: Affiliation: 1. Fisheries College, Jimei University, Xiamen 361021, China;2. Engineering Research Center of the Modern Industry Technology for Eel, Ministry of Education, Xiamen 361021, China;3. College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China;4. Technical Center of Xiamen Entry-Exit Inspection and Quarantine Bureau, Xiamen 361026, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:在水温(21±1)℃下,给体重(592.5±52.5)g的日本鳗鲡()雌亲鱼投喂每千克体重含0.034 g淫羊藿和0.034 g菟丝子浸膏的饲料90 d,对照组投喂人工配合饲料,研究淫羊藿和菟丝子对卵巢发育的影响。结果显示,饲料中添加淫羊藿和菟丝子的雌鱼卵巢大部分卵母细胞属第Ⅱ时相,性腺指数和肝体比均显著高于对照组(<0.05);卵母细胞油滴明显增多,部分卵母细胞胞质已充满油滴,核仁变小增多;血清雌二醇(E2)和11-酮基睾酮(11-KT)水平显著升高(cyp19a1仅微量表达,肝脏未检测到的表达;实验组肌肉总脂肪酸、饱和脂肪酸(SFA)、单不饱和脂肪酸(MUFA)和高度不饱和脂肪酸(HUFA)含量均显著高于对照组(<0.05)。本研究表明,在亲鱼培育饲料中添加中草药淫羊藿和菟丝子促进了日本鳗鲡卵母细胞油滴和肝脏卵黄蛋白原增加,提高了肌肉高不饱和脂肪酸的积累,为卵黄生成和卵母细胞进一步发育做好更充分的准备。 Abstract:In the present study, female Japanese eels () with an average body weight of (592.5±52.5) g were fed with a commercial diet supplemented with two Chinese herbs Lam at a water temperature of (21±1)℃ for 90 d. Then, the structure of ovarian development; changes in serum 11-KT and E2 levels; mRNA expression levels of liver vitellogenin (), and ovarian aromatase (tissue section staining, immunoenzymatic assays, semi-quantitative PCR, and gas chromatography, respectively. The results showed that the gonadosomatic indices (GSIs) and the hepatosomatic indices (HISs) in the Japanese eels fed with diets containing Lam. were significantly higher than those in the control group (<0.05); the treated group also showed ovaries filled with advanced perinucleolar follicles and increased numbers of oil droplets, and significantly higher serum 11-KT and E2 levels ( mRNA was elevated in the liver of the treated group; however, mRNA levels were extremely low in the ovaries of both the control group and the treated group, and the expression of mRNA was not detected in the livers of the two groups. Total fatty acid levels, including saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and highly unsaturated fatty acids (HUFA), in muscle were significantly higher in the Japanese eels fed with diets containing Lam than in the control group fish (<0.05); particularly, the levels of arachidonic acid (AA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) were higher in the treated group. These findings indicated that the two Chinese herbs, Lam., induced early ovary development by increasing oil droplets of previtellogenic oocytes and promoted vitellogenin accumulation in the liver of parental female Japanese eels. Moreover, Lam. accelerated absorption of fatty acids, particularly HUFA, from diets, and subsequently their accumulation in the muscle, to prepare for vitellogenesis and further development of oocytes. 参考文献 相似文献 引证文献